Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| developmental regulation of the intestinal fgf19 system in domestic pigs. | fibroblast growth factor-19 (fgf19) is an emerging endocrine factor involved in the regulation of bile acid homeostasis and energy metabolism in rodents and humans. in pigs, however, the fgf19 system remains largely unexplored. this study was designed to investigate the developmental regulation of the fgf19 system in domestic pigs. samples of intestinal sections, liver, and plasma were collected from 24 pigs (n = 6) at four developmental stages (birth, pre-weaning, post-weaning, and adulthood). ... | 2018 | 29446652 |
| hepatitis e virus infection of slaughtered healthy pigs in brazil. | hepatitis e virus (hev) is an emerging pathogen that can be transmitted through contaminated raw or undercooked meat derived from domestic pigs. hev infections have been documented among pig herds, pig products and environmental samples raising concern about the spread of the virus. hev genotypes 3 and 4 are considered zoonotic and have been linked to human cases. hev was detected in 51 of 335 bile samples (15.2%) from healthy pigs in minas gerais, brazil. phylogenetic analysis of partial sequen ... | 2018 | 29441690 |
| the zbed6-igf2 axis has a major effect on growth of skeletal muscle and internal organs in placental mammals. | a single nucleotide substitution in the third intron of insulin-like growth factor 2 (igf2) is associated with increased muscle mass and reduced subcutaneous fat in domestic pigs. this mutation disrupts the binding of the zbed6 transcription factor and leads to a threefold up-regulation ofigf2expression in pig skeletal muscle. here, we investigated the biological significance of zbed6-igf2interaction in the growth of placental mammals using two mouse models, zbed6 knock-out (zbed6-/-) andigf2kno ... | 2018 | 29440408 |
| accurate depth of radiofrequency-induced lesions in renal sympathetic denervation based on a fine histological sectioning approach in a porcine model. | ablation lesion depth caused by radiofrequency-based renal denervation (rdn) was limited to <4 mm in previous animal studies, suggesting that radiofrequency-rdn cannot ablate a substantial percentage of renal sympathetic nerves. we aimed to define the true lesion depth achieved with radiofrequency-rdn using a fine sectioning method and to investigate biophysical parameters that could predict lesion depth. | 2018 | 29440276 |
| the effect of three different surgical techniques for colon anastomosis on regional postoperative microperfusion: laser doppler flowmetry study in pigs. | the optimal surgical approach to reconnecting bowel ends safely after resection is of great importance. | 2018 | 29439318 |
| genomic sequence of a swine pasivirus type 1 strain identified in u.s. swine. | we report for the first time in the united states the identification of a swine pasivirus (spav) strain with a genomic sequence identity of less than 80% to other spavs reported in europe and china, using a next-generation sequencing (ngs) technique in sow tissues collected from an animal study conducted in 2001, suggesting virus circulation in domestic swine. | 2018 | 29439048 |
| identification of two novel foot-and-mouth disease virus cytotoxic t lymphocyte epitopes that can bind six sla-i proteins. | currently available vaccines from inactivated foot-and-mouth disease virus (fmdv) only protect animals by inducing neutralizing antibodies. a vaccine that contains cytotoxic t lymphocytes (ctl) epitopes to induce strong ctl responses might protect animals more effectively. herein, we used swine leukocyte antigen class i (slai) proteins derived from six different strains of domestic pigs to screen and identify shared fmdv ctl epitopes. four potential fmdv ctl epitopes (q01, q02, as3, and qa4) wer ... | 2018 | 29432828 |
| aujeszky's disease and hepatitis e viruses transmission between domestic pigs and wild boars in corsica: evaluating the importance of wild/domestic interactions and the efficacy of management measures. | wildlife species as reservoirs of infectious pathogens represent a serious constraint in the implementation of disease management strategies. in the mediterranean island of corsica, the dynamics of hepatitis e virus (hev) and aujeszky's disease virus (adv) are suspected to be influenced by interactions between wild and domestic pigs. to improve our understanding of these influences, we first compared the seroprevalences of both viruses in domestic pig populations from different locations with co ... | 2018 | 29417054 |
| voluntary locomotor activity promotes myogenic growth potential in domestic pigs. | self-determined physical activity is an essential behavioural need and can vary considerably between individuals of a given species. although locomotion is suggested as a prerequisite for adequate function of skeletal muscle, domestic pigs are usually reared under limited space allowance. the aim of our study was to investigate if a different voluntary locomotor activity leads to altered properties in the muscle structure, biochemistry and mrna expression of selected genes involved in myogenesis ... | 2018 | 29416067 |
| genetic variation analysis of pcv1 strains isolated from guangxi province of china in 2015. | porcine circovirus type 1 (pcv1) was discovered in 1974 as a contaminant of a porcine kidney (pk-15) cell line and was generally accepted to be nonpathogenic. but recently it was shown to cause lesions in experimentally infected pig fetuses. serological evidence and genetic studies suggested that pcv1 was widespread in domestic pigs. thus, the molecular epidemiology and genetic variation of pcv1 are still necessary to understand. | 2018 | 29415728 |
| suitability of the porcine aortic model for transcatheter aortic root repair. | to treat aortic valve disease and concomitant root disease with transcatheter techniques, 'composite graft' implants are required. our goal was to assess the suitability of the porcine aortic root for transcatheter root repair tests. | 2018 | 29415164 |
| observational analysis on inflammatory reaction to talc pleurodesis: small and large animal model series review. | talc pleurodesis has been associated with pleuropulmonary damage, particularly long-term damage due to its inert nature. the present model series review aimed to assess the safety of this procedure by examining inflammatory stimulus, biocompatibility and tissue reaction following talc pleurodesis. talc slurry was performed in rabbits: 200 mg/kg checked at postoperative day 14 (five models), 200 mg/kg checked at postoperative day 28 (five models), 40 mg/kg, checked at postoperative day 14 (five m ... | 2018 | 29403549 |
| the development of an interactive computer-based training program for timely and humane on-farm pig euthanasia. | with extensive knowledge and training in the prevention, management, and treatment of disease conditions in animals, veterinarians play a critical role in ensuring good welfare on swine farms by training caretakers on the importance of timely euthanasia. to assist veterinarians and other industry professionals in training new and seasoned caretakers, an interactive computer-based training program was created. it consists of three modules, each containing five case studies, which cover three dist ... | 2018 | 29400634 |
| efficacy and safety of sic-8000 (eleview®) for submucosal injection for endoscopic mucosal resection and endoscopic submucosal dissection in an in vivo porcine model. | submucosal injection is generally required for both endoscopic-mucosal resection (emr) and submucosal dissection (esd). sic-8000 (eleview™) is a new fda 510(k) cleared and ce marked liquid composition for submucosal injection, containing a biocompatible polymer as a cushioning agent. | 2017 | 29396133 |
| immunisation of pigs by dna prime and recombinant vaccinia virus boost to identify and rank african swine fever virus immunogenic and protective proteins. | african swine fever virus (asfv) causes an acute haemorrhagic fever in domestic pigs with high socio-economic impact. no vaccine is available limiting options for control. although live attenuated asfv can induce up to one hundred percent protection against lethal challenge, little is known of the antigens which induce this protective response. to identify additional asfv immunogenic and potentially protective antigens we cloned 47 viral genes in individual plasmids for gene vaccination and in r ... | 2018 | 29386289 |
| a serologic survey of pathogens in wild boar ( sus scrofa) in sweden. | the wild boar ( sus scrofa) population has increased markedly during the last three decades in sweden and in other parts of europe. this population growth may lead to increased contact between the wild boar and the domestic pig ( sus scrofa scrofa), increasing the risk of transmission of pathogens. the objective of our study was to estimate the seroprevalence of selective pathogens, known to be shared between wild boars and domestic pigs in europe, in three wild boar populations in sweden. in to ... | 2018 | 29377751 |
| viability and biocompatibility of an adhesive system for intrarenal embedding and endoscopic removal of small residual fragments in minimally-invasive stone treatment in an in vivo pig model. | to evaluate the viability and biocompatibility of a novel, patented bioadhesive system for intrarenal embedding and retrieval of residual fragments after endoscopic lithotripsy. complete stone clearance via active removal of residual fragments (rf) after intracorporeal laser lithotripsy may be time-consuming and fail in many cases. therefore, the novel adhesive has been developed and evaluated for the first time in an in vivo pig model in the present work. | 2018 | 29368229 |
| genetic diversity, breed composition and admixture of kenyan domestic pigs. | the genetic diversity of african pigs, whether domestic or wild has not been widely studied and there is very limited published information available. available data suggests that african domestic pigs originate from different domestication centers as opposed to international commercial breeds. we evaluated two domestic pig populations in western kenya, in order to characterize the genetic diversity, breed composition and admixture of the pigs in an area known to be endemic for african swine fev ... | 2018 | 29357367 |
| gastrointestinal protists and helminths of habituated agile mangabeys (cercocebus agilis) at bai hokou, central african republic. | infectious diseases including those caused by parasites can be a major threat to the conservation of endangered species. there is thus a great need for studies describing parasite infections of these species in the wild. here we present data on parasite diversity in an agile mangabey (cercocebus agilis) group in bai hokou, dzanga-sangha protected areas (dspa), central african republic. we coproscopically analyzed 140 mangabey fecal samples by concentration techniques (flotation and sedimentation ... | 2018 | 29350404 |
| intrarenal fat deposition does not interfere with the measurement of single-kidney perfusion in obese swine using multi-detector computed tomography. | altered vascular structure or function in several diseases may impair renal perfusion. multi-detector computed tomography (mdct) is a non-invasive tool to assess single-kidney perfusion and function based on dynamic changes in tissue attenuation during contrast media transit. however, changes in basal tissue attenuation might hamper these assessments, despite background subtraction. evaluation of iodine concentration using the dual-energy (dect) mdct mode allows excluding effects of basal values ... | 2018 | 29339048 |
| the retina of the collared peccary (pecari tajacu): structure and function. | to study retinal morphology and function in the collared peccary, an ungulate species distantly related to the domestic pig. | 2018 | 29336116 |
| cardiovascular function of modern pigs does not comply with allometric scaling laws. | growing concerns have been expressed regarding cardiovascular performance in modern farm pigs, which has been proposed as a critical factor contributing to the reduced adaptability of modern pigs to stress. here we tested the hypothesis that cardiac dimensions and pump function in modern heavy farm pigs are disproportionally low for their body weight, and investigated potential underlying mechanisms. the results from the present study indeed demonstrate disproportionally low values for stroke vo ... | 2018 | 29335617 |
| first-time detection of porcine reproductive and respiratory syndrome virus (prrsv) infection in uruguay. | within the last two decades, several high-impact viruses have emerged in the global swine population, including porcine reproductive and respiratory syndrome virus (prrsv). in uruguay, the more recent serological survey for prrsv and other notifiable diseases such as aujeszky's disease virus (adv) and classical swine fever virus (csfv) dated from year 2000. the main purpose of this study was to update our information on the infection status of prrsv, adv and csfv in uruguayan pig herds, in order ... | 2018 | 29330937 |
| an assessment of shedding with the oral rabies virus vaccine strain spbn gasgas in target and non-target species. | a safety requirement for live vaccines is investigating possible shedding in recipients since the presence of replication competent vaccine in secretions could result in direct and indirect horizontal transmission. this is especially relevant for oral rabies vaccine baits that are deliberately distributed into the environment. in the current study, survival of an oral rabies virus vaccine, spbn gasgas, was examined in excretions from different target and non-target species; red fox, raccoon dog, ... | 2018 | 29325820 |
| elevated proportions of deleterious genetic variation in domestic animals and plants. | a fraction of genetic variants segregating in any population are deleterious, which negatively impacts individual fitness. the domestication of animals and plants is associated with population bottlenecks and artificial selection, which are predicted to increase the proportion of deleterious variants. however, the extent to which this is a general feature of domestic species is unclear. here, we examine the effects of domestication on the prevalence of deleterious variation using pooled whole-ge ... | 2018 | 29325102 |
| faecal escherichia coli as biological indicator of spatial interaction between domestic pigs and wild boar (sus scrofa) in corsica. | on the mediterranean island of corsica, cohabitation between sympatric domestic pigs and eurasian wild boar (sus scrofa) is common and widespread and can facilitate the maintenance and dissemination of several pathogens detrimental for the pig industry or human health. in this study, we monitored a population of free-ranging domestic pigs reared in extensive conditions within a 800-ha property located in central corsica which was frequently visited by a sympatric population of wild boar between ... | 2018 | 29322645 |
| detection of β-lactamase encoding genes in feces, soil and water from a brazilian pig farm. | β-lactam antibiotics are widely used for the treatment of different types of infections worldwide and the resistance to these antibiotics has grown sharply, which is of great concern. resistance to β-lactams in gram-negative bacteria is mainly due to the production of β-lactamases, which are classified according to their functional activities. the aim of this study was to verify the presence of β-lactamases encoding genes in feces, soil, and water from a brazilian pig farm. different β-lactamase ... | 2018 | 29322334 |
| assessing the utility of metabarcoding for diet analyses of the omnivorous wild pig (sus scrofa). | wild pigs (sus scrofa) are an invasive species descended from both domestic swine and eurasian wild boar that was introduced to north america during the early 1500s. wild pigs have since become the most abundant free-ranging exotic ungulate in the united states. large and ever-increasing populations of wild pigs negatively impact agriculture, sport hunting, and native ecosystems with costs estimated to exceed $1.5 billion/year within the united states. wild pigs are recognized as generalist feed ... | 2018 | 29321862 |
| a prototype single-port device for pressurized intraperitoneal aerosol chemotherapy. technical feasibility and local drug distribution. | to evaluate the technical feasibility and homogeneity of drug distribution of pressurized intraperitoneal aerosol chemotherapy (pipac) based on a novel process of intraperitoneal drug application (multidirectional aerosolization). | 2017 | 29319734 |
| intraoperative cardiovascular response of natural orifice transluminal endoscopic surgery versus laparoscopy: a comparative animal study. | natural orifice transluminal endoscopic surgery (notes) emerged as a new alternative method in minimally invasive techniques. although a very small number of studies have compared the physiologic response in notes to laparoscopy, the results remain controversial. | 2018 | 29319013 |
| genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central china. | porcine circovirus type 2 (pcv2) is an economically important pathogen in domestic pigs and wild boars all around the world. to understand the molecular epidemiology of pcv2 strains circulating in central china and to provide a potential vaccine candidate strain, we analyzed the genetic variations of 46 pcv2 isolates circulating from 2009 to 2016 in henan province (24 detected in the field from 2009-2013 and 22 from 2013-2016) and evaluated the efficacy of an isolate as a vaccine candidate strai ... | 2018 | 29305646 |
| species specific antiviral activity of porcine interferon-α8 (ifnα8). | interferons (ifns) have been known as antiviral genes and they are classified by type 1, type 2, and type 3 ifn. the type 1 ifn consists of ifnα, ifnβ, ifnτ, and ifnω whereas the type 2 ifn consists of only ifnγ, which is a key cytokine driving t helper cell type 1 immunity. ifnλ belongs to the type 3 ifn, which is also known as il-28 and il-29 possessing antiviral activities. type 1 ifn is produced by viral infection whereas type 2 ifn is induced by mitogenic or antigenic t-cell stimuli. the if ... | 2017 | 29302255 |
| host contributes to longitudinal diversity of fecal microbiota in swine selected for lean growth. | in pigs, gut bacteria have been shown to play important roles in nutritional, physiological, and immunological processes in the host. however, the contribution of their metagenomes or part of them, which are normally reflected by fragments of 16s rrna-encoding genes, has yet to be fully investigated. | 2018 | 29301569 |
| serological and virological survey of hepatitis e virus (hev) in animal reservoirs from uruguay reveals elevated prevalences and a very close phylogenetic relationship between swine and human strains. | hepatitis e virus (hev) infection is an issue of public health concern in high-income and non-endemic countries. increasing evidence supports the hypothesis of a zoonotic route as the main mode of infection in this epidemiological setting, since the transmission of genotypes hev-3 and hev-4 from reservoirs to humans has been demonstrated. in america, studies have confirmed the circulation of hev in pig herds but the zoonotic role of wild boars has never been evaluated. uruguay has a high burden ... | 2018 | 29291999 |
| feasibility study of a novel thoraco-abdominal aortic hybrid device (spider-graft) in a translational pig model. | the hybrid spider-graft consists of a proximal descending aortic stent graft and a conventional six branched dacron graft for open abdominal aortic repair. technical feasibility with regard to avoiding thoracotomy and extracorporeal circulation (ecc) during thoraco-abdominal aortic hybrid repair and peri-procedural safety of this novel device are unknown. | 2018 | 29290476 |
| magnetic resonance elastography can monitor changes in medullary stiffness in response to treatment in the swine ischemic kidney. | low-energy shockwave (sw) therapy attenuates damage in the stenotic kidney (stk) caused by atherosclerotic renal artery stenosis (aras). we hypothesized that magnetic resonance elastography (mre) would detect attenuation of fibrosis following sw in unilateral aras kidneys. | 2017 | 29289980 |
| detection of hepatitis e virus genotypes 3 and 4 in pig farms in korea. | the zoonotic transmission of hepatitis e virus (hev) is mostly mediated by hev-3 and hev-4, and domestic pigs are an important reservoir for these genotypes. a survey of 14 pig farms in korea revealed hev rna in 30 of 148 (20.3%) fecal samples. hev-3a and hev-4c subtypes were identified from five (36%) and two (14%) pig farms, respectively. phylogenetic analysis indicated that the isolated hev strains were closely related with previously reported zoonotic strains in korea. this genetic analysis ... | 2017 | 29284214 |
| miniature pig model of human adolescent brain white matter development. | neuroscience research in brain development and disorders can benefit from an in vivo animal model that portrays normal white matter (wm) development trajectories and has a sufficiently large cerebrum for imaging with human mri scanners and protocols. | 2017 | 29277719 |
| mitochondrial targeted peptides preserve mitochondrial organization and decrease reversible myocardial changes in early swine metabolic syndrome. | the mechanisms responsible for cardiac damage in the early stages of metabolic syndrome (mets) remain unknown. mitochondria are intimately associated with cellular myofibrils, with the cytoskeleton functioning as a linkage coordinator, and closely associated to the calcium release sites of the sarcoplasmic reticulum (sr). objective: we hypothesized that early mets is characterized by mitochondria-related myocardial damage, associated with altered cytoskeletal-mitochondria-sr interaction. methods ... | 2017 | 29267873 |
| effects of autologous fat and ascs on swine hypertrophic burn scars: a multimodal quantitative analysis. | hypertrophic scar formation is unpredictable and poorly understood, afflicting both the pediatric and adult populations. treatment methods with conservative and invasive approaches have low rates of compliance and high rates of morbidity. the purpose of this study was to test a reproducible scar model and investigate a new technique of scar modification through the use of adipose- derived progenitor stromal cells (ascs). | 2017 | 29263956 |
| spontaneously arrested transmission of cysticercosis in a highly endemic village with a very low migration rate. | taenia soliumcysticercosis is difficult to eliminate without interventions or societal development. atahualpa is a rural ecuadorian village with documented low migration rate, where domestic pig raising is common and human cysticercosis is endemic. to assess neurocysticercosis (ncc) prevalence, 1,273 villagers aged ≥ 20 years underwent neuroimaging studies, which showed calcified lesions in 121 (9.5%) individuals, but no active disease. likewise, positive reactions, apparently nonspecific, were ... | 2017 | 29260655 |
| the silent spread of porcine bocavirus in croatian pigs: should we be concerned? | a survey was conducted to evaluate the presence and prevalence of porcine bocavirus (pbov) in croatian domestic pigs by means of pcr targeting the ns1 gene fragment of pbov. this study included testing of faecal samples collected from 10 small commercial farms and 11 small backyard holdings in croatia. the presence of pbov was confirmed by pcr in 24 out of 57 composite faecal samples from small commercial farms and in 12 out of 43 composite faecal samples from small backyard holdings. the pcr pr ... | 2017 | 29256289 |
| inferring the evolution of the major histocompatibility complex of wild pigs and peccaries using hybridisation dna capture-based sequencing. | the major histocompatibility complex (mhc) is a key genomic model region for understanding the evolution of gene families and the co-evolution between host and pathogen. to date, mhc studies have mostly focused on species from major vertebrate lineages. the evolution of mhc classical (ia) and non-classical (ib) genes in pigs has attracted interest because of their antigen presentation roles as part of the adaptive immune system. the pig family suidae comprises over 18 extant species (mostly wild ... | 2017 | 29256177 |
| modulation of the main porcine enteric neuropeptides by a single low-dose of lipopolysaccharide (lps) salmonella enteritidis. | the present research was conducted to investigate the influence of a low, single dose of lps, which does not result in any clinical symptoms of intoxication on the expression of selected neuropeptides within the intestines of the domestic pig. | 2017 | 29255488 |
| evaluation of protection induced by immunisation of domestic pigs with deletion mutant african swine fever virus beninδmgf by different doses and routes. | a live attenuated african swine fever virus (asfv) vaccine candidate, produced by deletion of several genes belonging to multi-gene families mgf360 and 505 from virulent benin 97/1 strain (beninδmgf), induces protection in pigs against parental virulent strain. in order to better define the safety and efficacy of this attenuated vaccine candidate and to understand protective mechanisms, we extended previous studies by intramuscular immunisation of pigs with the deletion mutant beninδmfg at diffe ... | 2018 | 29254837 |
| questionnaire-based assessment of wild boar/domestic pig interactions and implications for disease risk management in corsica. | wild boars and domestic pigs belong to the same species (sus scrofa). when sympatric populations of wild boars, feral pigs, and domestic pigs share the same environment, interactions between domestic and wild suids (idws) are suspected to facilitate the spread and maintenance of several pig pathogens which can impact on public health and pig production. however, information on the nature and factors facilitating those idws are rarely described in the literature. in order to understand the occurr ... | 2017 | 29250528 |
| prevalence and risk factors of japanese encephalitis virus (jev) in livestock and companion animal in high-risk areas in malaysia. | japanese encephalitis (je) is vector-borne zoonotic disease which causes encephalitis in humans and horses. clinical signs for japanese encephalitis virus (jev) infection are not clearly evident in the majority of affected animals. in malaysia, information on the prevalence of jev infection has not been established. thus, a cross-sectional study was conducted during two periods, december 2015 to january 2016 and march to august in 2016, to determine the prevalence and risk factors in jev infecti ... | 2017 | 29243139 |
| a nationwide serosurvey of hepatitis e virus antibodies in the general population of portugal. | evidence has shown that hepatitis e virus (hev) genotype 3 is autochthonous in industrialized countries due to zoonotic transmission through direct contact or consumption of raw or undercooked meat from domestic swine or wild boar. as there is lack of data on seroprevalence of hev in the general portuguese population, a wide survey was conducted as part of the hepecontrol project (60dt2), under eea grants funding. | 2017 | 29237007 |
| toxoplasma gondii seroprevalence in breeding pigs in estonia. | toxoplasma gondii is a widespread occurring parasite infecting warm-blooded animals, including pigs and humans. the aims of this study were to estimate the prevalence of anti-t. gondii antibodies and to evaluate risk factors for t. gondii seropositivity in breeding pigs raised in estonia. sera from 382 pigs were tested with a commercial direct agglutination test, using a cut-off titer of 40 for seropositivity, for the presence of anti-t. gondii immunoglobulin g antibodies. | 2017 | 29228997 |
| comparison of continuous compression with regular ventilations versus 30:2 compressions-ventilations strategy during mechanical cardiopulmonary resuscitation in a porcine model of cardiac arrest. | a compression-ventilation (c:v) ratio of 30:2 is recommended for adult cardiopulmonary resuscitation (cpr) by the current american heart association (aha) guidelines. however, continuous chest compression (ccc) is an alternative strategy for cpr that minimizes interruption especially when an advanced airway exists. in this study, we investigated the effects of 30:2 mechanical cpr when compared with ccc in combination with regular ventilation in a porcine model. | 2017 | 29221300 |
| hepatitis e seroprevalence and genotyping in a cohort of wild boars in southern germany and eastern alsace. | in the last few years it has been realized that the hepatitis e virus (hev) is endemic in most industrialized countries and that it is a zoonotic disease. potential reservoirs for hev have been identified to be wild boars and deers, but hev has also been found in domestic pigs and other animals. due to the probable spread of the virus via contaminated food or contact to infected animals, hev antibodies are present in more than 16% of the german adult population and rates are increasing with age. ... | 2017 | 29214558 |
| effects of intramuscularly administered enrofloxacin on the susceptibility of commensal intestinal escherichia coli in pigs (sus scrofa domestica). | in the european union, various fluoroquinolones are authorised for the treatment of food producing animals. each administration poses an increased risk of development and spread of antimicrobial resistance. the aim of this study was to investigate the impact of parenteral administration of enrofloxacin on the prevalence of enrofloxacin and ciprofloxacin susceptibilities in the commensal intestinal e. coli population. | 2017 | 29202759 |
| compression sonography for non-invasive measurement of lower leg compartment pressure in an animal model. | compression ultrasound is a non-invasive technique allowing for qualitative visualization and quantitative measurements of mechanical tissue properties. in acute compartment syndrome (acs), cadaver studies have proven that the intra-compartmental pressure (icp) measured by compression sonography correlates with the icp measured invasively. this study aimed to evaluate compression sonography for compartment pressure measurements in an animal model. | 2017 | 29195681 |
| behavioural lateralization in domestic pigs (sus scrofa)-variations between motor functions and individuals. | motor lateralization is hypothesized to depend on the complexity of the motor function, but it might at the same time reflect hemispheric dominance within an individual across motor functions. we investigated possible motor lateralization patterns in four motor functions of different complexity (snout use in a manipulative task, foot use in two-stepping tasks and tail curling) in the domestic pig, a tetrapod species relevant as farm animal but also as a model in human neuroscience. a significant ... | 2017 | 29191074 |
| serological evidence of hepatitis e virus infection in zoo animals and identification of a rodent-borne strain in a syrian brown bear. | hepatitis e virus (hev) is the causative agent of hepatitis e, an emerging infectious disease of humans. hev infections have also been described in various animal species. whereas domestic pigs and wild boars are well-known animal reservoirs for hev, the knowledge on natural hev infection in zoo animals is scarce so far. here, we analysed 244 sera from 66 mammal species derived from three zoos in germany using a commercial double antigen sandwich elisa. hev-specific antibodies were detected in 1 ... | 2017 | 29173594 |
| characterization of domestic pig spermatogenesis using spermatogonial stem cell markers in the early months of life. | in pigs, spermatogonial stem cells (sscs), which include gonocytes and undifferentiated spermatogonia, are classically defined as being present till up to 2 months of life. however, knowledge about this transition from gonocytes to undifferentiated spermatogonia in pigs is limited. in this study, we characterized pig sscs in neonatal (n = 6), one-month-old (1-mo-old, n = 6), two-month-old (2-mo-old, n = 6), and adult testes (n = 6) anatomically, histologically, and immunohistologically. anatomic ... | 2018 | 29156418 |
| outbreaks of neuroinvasive astrovirus associated with encephalomyelitis, weakness, and paralysis among weaned pigs, hungary. | a large, highly prolific swine farm in hungary had a 2-year history of neurologic disease among newly weaned (25- to 35-day-old) pigs, with clinical signs of posterior paraplegia and a high mortality rate. affected pigs that were necropsied had encephalomyelitis and neural necrosis. porcine astrovirus type 3 was identified by reverse transcription pcr and in situ hybridization in brain and spinal cord samples in 6 animals from this farm. among tissues tested by quantitative rt-pcr, the highest v ... | 2017 | 29148391 |
| gene expression analyses of the small intestine of pigs in the ex-evacuation zone of the fukushima daiichi nuclear power plant. | after the accident at the fukushima daiichi nuclear power plant, radioactive contaminants were released over a widespread area. monitoring the biological effects of radiation exposure in animals in the ex-evacuation zone should be continued to understand the health effects of radiation exposure in humans. the present study aimed to clarify the effects of radiation by investigating whether there is any alteration in the morphology and gene expressions of immune molecules in the intestine of pigs ... | 2017 | 29141628 |
| in vivo assessment of a novel biodegradable ureteral stent. | to perform an in vivo assessment of a newly developed biodegradable ureteral stent (bus) produced with natural-based polymers. | 2018 | 29128964 |
| preclinical animal models for temporomandibular joint tissue engineering. | there is a paucity of in vivo studies that investigate the safety and efficacy of temporomandibular joint (tmj) tissue regeneration approaches, in part due to the lack of established animal models. review of disease models for study of tmj is presented herein with an attempt to identify relevant preclinical animal models for tmj tissue engineering, with emphasis on the disc and condyle. although degenerative joint disease models have been mainly performed on mice, rats, and rabbits, preclinical ... | 2018 | 29121815 |
| metabolic syndrome alters expression of insulin signaling-related genes in swine mesenchymal stem cells. | metabolic syndrome (mets) is associated with insulin resistance (ir) and impaired glucose metabolism in muscle, fat, and other cells, and may induce inflammation and vascular remodeling. endogenous reparative systems, including adipose tissue-derived mesenchymal stem/stromal cells (msc), are responsible for repair of damaged tissue. msc have also been proposed as an exogenous therapeutic intervention in patients with cardiovascular and chronic kidney disease (ckd). the feasibility of using autol ... | 2018 | 29101070 |
| utilization of mri for cerebral white matter injury in a hypobaric swine model-validation of technique. | repetitive hypobaric exposure in humans induces subcortical white matter change, observable on magnetic resonance imaging (mri) and associated with cognitive impairment. similar findings occur in traumatic brain injury (tbi). we are developing a swine mri-driven model to understand the pathophysiology and to develop treatment interventions. | 2017 | 29087921 |
| tracing the breeding farm of domesticated pig using feature selection (sus scrofa). | increasing food safety demands in the animal product market have created a need for a system to trace the food distribution process, from the manufacturer to the retailer, and genetic traceability is an effective method to trace the origin of animal products. in this study, we successfully achieved the farm tracing of 6,018 multi-breed pigs, using single nucleotide polymorphism (snp) markers strictly selected through least absolute shrinkage and selection operator (lasso) feature selection. | 2017 | 29073733 |
| genome-wide analysis of structural variants reveals genetic differences in chinese pigs. | pigs have experienced long-term selections, resulting in dramatic phenotypic changes. structural variants (svs) are reported to exert extensive impacts on phenotypic changes. we built a high resolution and informative sv map based on high-depth sequencing data from 66 chinese domestic and wild pigs. we inferred the sv formation mechanisms in the pig genome and used svs as materials to perform a population-level analysis. we detected the selection signals on chromosome x for northern chinese dome ... | 2017 | 29065176 |
| production, performance, slaughter characteristics, and meat quality of ziwuling wild crossbred pigs. | wild boars, because of their large size and ability to survive adverse conditions, are usually used to cross with domestic breeds to improve the quality of domesticated pigs. this study aimed to investigate the growth performance, slaughter performance, and meat quality of ziwuling crossbred pigs. crossbred pigs in four groups (n = 8 per group, 4 boars and 4 sows, all aged 100 days), f1[wild × b (bamei)], f1 × b, f1 × y (yorkshire), and f1 × f1, were selected at a commercial pig farm. growth per ... | 2018 | 29064063 |
| reliability of transcardiopulmonary thermodilution cardiac output measurement in experimental aortic valve insufficiency. | monitoring cardiac output (co) is important to optimize hemodynamic function in critically ill patients. the prevalence of aortic valve insufficiency (ai) is rising in the aging population. however, reliability of co monitoring techniques in ai is unknown. the aim of this study was to investigate the impact of ai on accuracy, precision, and trending ability of transcardiopulmonary thermodilution-derived cotcptd in comparison with pulmonary artery catheter thermodilution copac. | 2017 | 29049339 |
| population admixture in chinese and european sus scrofa. | relationships between different populations were investigated using porcine 60 k data from 1,135 domestic pigs and wild boars across europe and china. the results indicate that most european breeds have been introgressed with chinese ancestry, but the extent of introgression varies considerably among breeds. moreover, the main source of this introgression is pigs from south china, closely related to bamaxiang and dongshan pigs. contributions from east and central chinese pig breeds are also dete ... | 2017 | 29030577 |
| simultaneous profiles of sulfonated androgens, sulfonated estrogens and sulfonated progestogens in postpubertal boars (sus scrofa domestica) measured by lc-ms/ms. | sulfonated steroids (s-st) have been usually regarded as inactive metabolites but are progressively considered as precursors for the intra-tissue formation of bioactive steroids. moreover, independent effects without preceding removal of the sulfate group have been observed. we use the porcine testicular-epididymal compartment as a model to investigate the still largely unknown s-st physiology as the boar exhibits an intriguingly broad s-st spectrum predominantly originating from the testis. the ... | 2017 | 29030154 |
| biocompatibility and osseointegration of titanium implants with a silver-doped polysiloxane coating: an in vivo pig model. | to test the antimicrobial properties, surface topography, reaction of surrounding tissue (biocompatibility), and osseointegration of ultrathin implant surfaces containing polysiloxane and nanoscaled silver particles. | 2017 | 29028852 |
| why is african swine fever still present in sardinia? | african swine fever (asf) is an infectious disease of swine that has been present in sardinia since 1978. soon after introduction of the disease, several control and eradication programmes were established with limited success. some researchers attributed the persistence of the disease in central and eastern areas to certain socio-economic factors, the existence of some local and traditional farming practices (i.e., unregistered free-ranging pigs known as brado animals) and the high density of w ... | 2017 | 29027378 |
| characterization of the african swine fever virus decapping enzyme during infection. | african swine fever virus (asfv) infection is characterized by a progressive decrease in cellular protein synthesis with a concomitant increase in viral protein synthesis, though the mechanism by which the virus achieves this is still unknown. decrease of cellular mrna is observed during asfv infection, suggesting that inhibition of cellular proteins is due to an active mrna degradation process. asfv carries a gene (ba71v d250r/malawi g5r) that encodes a decapping protein (asfv-dp) that has a nu ... | 2017 | 29021398 |
| a preclinical evaluation comparing the performance of a novel 19-g flexible needle to a commercially available 22-g ebus-tbna sampling needle. | needle samples may not provide sufficient diagnostic material for the assessment of mediastinal lymph nodes. | 2017 | 29020679 |
| diurnal rhythms in peripheral blood immune cell numbers of domestic pigs. | diurnal rhythms within the immune system are considered important for immune competence. until now, they were mostly studied in humans and rodents. however, as the domestic pig is regarded as suitable animal model and due to its importance in agriculture, this study aimed to characterize diurnal rhythmicity in porcine circulating leukocyte numbers. eighteen pigs were studied over periods of up to 50 h. cosinor analyses revealed diurnal rhythms in cell numbers of most investigated immune cell pop ... | 2018 | 29017838 |
| signatures of selection and interspecies introgression in the genome of chinese domestic pigs. | chinese domestic pigs have experienced strong artificial selection for thousands of years. however, the molecular mechanisms underlying the selection-causing phenotypic changes in chinese domestic pigs are still largely unknown. here we used whole-genome resequencing data of 54 pigs from 9 chinese diverse breeds and 16 wild boars from 7 localities across china to identify genes that show evidence of positive selection in the process of domestication. a total of 14 candidate domestication regions ... | 2017 | 29016799 |
| improved method for the establishment of an in vitro blood-brain barrier model based on porcine brain endothelial cells. | the aim of this protocol presents an optimized procedure for the purification and cultivation of pbecs and to establish in vitro blood-brain barrier (bbb) models based on pbecs in mono-culture (mc), mc with astrocyte-conditioned medium (acm), and non-contact co-culture (ncc) with astrocytes of porcine or rat origin. pbecs were isolated and cultured from fragments of capillaries from the brain cortices of domestic pigs 5-6 months old. these fragments were purified by careful removal of meninges, ... | 2017 | 28994773 |
| ex vivo treatment with inhaled n-acetylcysteine in porcine lung transplantation. | we have shown the beneficial effects of n-acetylcysteine (nac) on posttransplant lung function, when both donor and recipient were pretreated intravenously. however, systemic treatment of multiorgan donors may not be clinically relevant. thus, we hypothesized that ex vivo treatment of donors with nebulized nac would be adequate to prevent from ischemia-reperfusion injury after lung transplantation. | 2017 | 28985871 |
| molecular study of porcine circovirus type 2 in wild boars and domestic pigs in uruguay from 2010 to 2014: predominance of recombinant circulating strains. | porcine circovirus type 2 (pcv2) is a worldwide distributed pathogen and one of the most economically relevant swine infections. four genotypes have been recognized and it is well known that pcv2a, pcv2b and pcv2d have a global distribution. however, the information about recombinant strains circulation and their influence in driving pcv2 evolution is a poorly studied area. in uruguay, pcv2 associated symptoms began to be frequently observed in pigs from different farms since 2010. the main purp ... | 2017 | 28964894 |
| complete genome sequences and phylogenetic analysis of encephalomyocarditis virus strains isolated from pigs and rats origin. | in order to evaluate the genetic variability of encephalomyocarditis virus (emcv), the whole genomes of six emcv field isolates originating from pigs and rats origin in different regions of central china, were phylogenetically and comparatively analyzed. phylogenetic analysis of whole genome sequences, open reading frame (orf), capsid coding region (ccr) and vp3/vp1 using neighbor-joining analysis revealed that these isolates belonged to lineage 1. nucleotide sequences of six isolates showed mor ... | 2017 | 28964856 |
| detection of toxoplasma gondii in naturally infected domestic pigs in northern serbia. | insufficiently cooked pork is considered as an important source of human infection with toxoplasma gondii. the aim of our study was to investigate the presence of t. gondii in pigs intended for human consumption from northern serbia. blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. sera were examined using modified agglutination test (mat), and diaphragms from seropositive, as well as from some ma ... | 2017 | 28956155 |
| interventional magnetic resonance imaging guided carotid embolectomy using a novel resonant marker catheter: demonstration of preclinical feasibility. | to assess the visualization and efficacy of a wireless resonant circuit (wrc) catheter system for carotid artery occlusion and embolectomy under real-time mri guidance in vivo, and to compare mr imaging modality with x-ray for analysis of qualitative physiological measures of blood flow at baseline and after embolectomy. the wrc catheter system was constructed using a mr compatible peek fiber braided catheter (penumbra, inc, alameda, ca) with a single insulated longitudinal copper loop soldered ... | 2017 | 28948399 |
| hepatitis e virus and related viruses in wild, domestic and zoo animals: a review. | hepatitis e is a human disease mainly characterized by acute liver illness, which is caused by infection with the hepatitis e virus (hev). large hepatitis e outbreaks have been described in developing countries; however, the disease is also increasingly recognized in industrialized countries. mortality rates up to 25% have been described for pregnant women during outbreaks in developing countries. in addition, chronic disease courses could be observed in immunocompromised transplant patients. wh ... | 2018 | 28944602 |
| peri-conceptional under-nutrition alters transcriptomic profile in the endometrium during the peri-implantation period-the study in domestic pigs. | female under-nutrition during early pregnancy may affect the physiological pattern of the transcriptomic profile in the endometrium. we aimed to determine if restricted diet applied to females during peri-conceptional period, that is, from the onset of the oestrus until day nine of pregnancy, alters transcriptomic profile in the endometrium during the peri-implantation period. the restricted diet gilts were fed forage, in which the dose of proteins and energy had been reduced by 30% compared to ... | 2018 | 28944507 |
| single step genome-wide association studies based on genotyping by sequence data reveals novel loci for the litter traits of domestic pigs. | in this study, data genotyping by sequence (gbs) was used to perform single step gwas (ssgwas) to identify snps associated with the litter traits in domestic pigs and search for candidate genes in the region of significant snps. after quality control, 167,355 high-quality snps from 532 pigs were obtained. phenotypic traits on 2112 gilt litters from 532 pigs were recorded including total number born (tnb), number born alive (nba), and litter weight born alive (lwb). a single-step genomic blup app ... | 2017 | 28943389 |
| multiplex real-time polymerase chain reaction for the differential detection of porcine circovirus 2 and 3. | a multiplex quantitative real-time polymerase chain reaction (mqpcr) assay was developed for the rapid and differential detection of porcine circovirus 2 (pcv2) and pcv3. each of the capsid genes of pcv2 and pcv3 were amplified using specific primers and probe sets, while no other porcine pathogen genes were detected. limit of detection of the assay was below 50 copies of the target genes of pcv2 and pcv3, and was comparable to that of previously described methods the assay showed high repeatabi ... | 2017 | 28941615 |
| seroprevalence of anti-hepatitis e virus antibodies in domestic pigs in mexico. | hepatitis e virus (hev) infection is one of the most common causes of acute liver diseases in humans worldwide. in developing countries, hev is commonly associated with waterborne outbreaks. conversely, in industrialized countries, hev infection is often associated with travel to endemic regions or ingestion of contaminated animal products. limited information on both, human and animal hev infection in mexico is available. as a consequence, the distribution of the virus in the country is largely ... | 2017 | 28934965 |
| genome-wide snp data unveils the globalization of domesticated pigs. | pigs were domesticated independently in eastern and western eurasia early during the agricultural revolution, and have since been transported and traded across the globe. here, we present a worldwide survey on 60k genome-wide single nucleotide polymorphism (snp) data for 2093 pigs, including 1839 domestic pigs representing 122 local and commercial breeds, 215 wild boars, and 39 out-group suids, from asia, europe, america, oceania and africa. the aim of this study was to infer global patterns in ... | 2017 | 28934946 |
| genetic characterization of african swine fever virus isolates from soft ticks at the wildlife/domestic interface in mozambique and identification of a novel genotype. | african swine fever virus (asfv) is one of the most threatening infectious diseases of pigs. there are not sufficient data to indicate the importance of the sylvatic cycle in the spread and maintenance of the disease locally and potentially, globally. to assess the capacity to maintain asf in the environment, we investigated the presence of soft tickreservoirs of asfv in gorongosa national park (gnp) and its surrounding villages. a total of 1,658 soft ticks were recovered from warthog burrows an ... | 2017 | 28921895 |
| diagnostic specificity of the african swine fever virus antibody detection enzyme-linked immunosorbent assay in feral and domestic pigs in the united states. | african swine fever (asf) is a highly contagious haemorrhagic disease of pigs that has the potential to cause mortality nearing 100% in naïve animals. while an outbreak of asf in the united states' pig population (domestic and feral) has never been reported, an introduction of the disease has the potential to cause devastation to the pork industry and food security. during the recovery phase of an outbreak, an antibody detection diagnostic assay would be required to prove freedom of disease with ... | 2017 | 28921873 |
| vocal correlates of emotional reactivity within and across contexts in domestic pigs (sus scrofa). | vocalizations have long been recognized to encode information about an individual's emotional state and, as such, have contributed to the study of emotions in animals. however, the potential of vocalizations to also encode information about an individual's emotional reactivity has received much less attention. in this study, we aimed to test whether the vocalizations of domestic pigs contain correlates of emotional reactivity that are consistent between different contexts. we recorded vocalizati ... | 2017 | 28912089 |
| persistence of mycoplasma hyopneumoniae sequence types in spite of a control program for enzootic pneumonia in pigs. | enzootic pneumonia (ep) in pigs caused by mycoplasma (m.) hyopneumoniae has successfully been combatted in switzerland. a control program was fully implemented in 2004 which is based on total depopulation strategies of affected fattening farms as well as partial depopulation on breeding farms. thereby, the number of cases has dropped drastically from more than 200 in 2003 to two cases in 2013. currently monitoring is done based on clinical observation and subsequent diagnostic of coughing pigs. ... | 2017 | 28903876 |
| evolution and genome specialization of brucella suis biovar 2 iberian lineages. | swine brucellosis caused by b. suis biovar 2 is an emergent disease in domestic pigs in europe. the emergence of this pathogen has been linked to the increase of extensive pig farms and the high density of infected wild boars (sus scrofa). in portugal and spain, the majority of strains share specific molecular characteristics, which allowed establishing an iberian clonal lineage. however, several strains isolated from wild boars in the north-east region of spain are similar to strains isolated i ... | 2017 | 28899413 |
| evidence for a primate origin of zoonotic helicobacter suis colonizing domesticated pigs. | helicobacter suis is the second most prevalent helicobacter species in the stomach of humans suffering from gastric disease. this bacterium mainly inhabits the stomach of domesticated pigs, in which it causes gastric disease, but it appears to be absent in wild boars. interestingly, it also colonizes the stomach of asymptomatic rhesus and cynomolgus monkeys. the origin of modern human-, pig- or non-human primate-associated h. suis strains in these respective host populations was hitherto unknown ... | 2017 | 28885626 |
| reduced virulence of a pseudorabies virus isolate from wild boar origin in domestic pigs correlates with hampered visceral spread and age-dependent reduced neuroinvasive capacity. | morbidity and mortality associated with pseudorabies virus (prv) infection are dependent on the age of the pig and the virulence of the strain. prv strains circulating in wild boar are considered to be low virulent, but no mechanistic explanation for their reduced virulence is available. here infection of 2- and 15-week-old domestic pigs with the prv wild boar strain bel24043 did not induce clinical symptoms in 15-week-old pigs, but resulted in important neurological and respiratory disease in 2 ... | 2017 | 28873002 |
| immortalization and characterization of porcine macrophages that had been transduced with lentiviral vectors encoding the sv40 large t antigen and porcine telomerase reverse transcriptase. | the domestic pig is an important agricultural animal, and thus, infectious diseases that affect pigs can cause severe economic losses in the global swine industry. various porcine pathogens target macrophages, which are classical innate immune cells. although macrophages basically protect the host from pathogens, they also seem to contribute to infectious processes. therefore, cultured macrophages can be used to developin vitromodels for studying not only genes associated with porcine innate imm ... | 2017 | 28871285 |
| a novel culture method reveals unique neural stem/progenitors in mature porcine iris tissues that differentiate into neuronal and rod photoreceptor-like cells. | iris neural stem/progenitor cells from mature porcine eyes were investigated using a new protocol for tissue culture, which consists of dispase treatment and matrigel embedding. we used a number of culture conditions and found an intense differentiation of neuronal cells from both the iris pigmented epithelial (ipe) cells and the stroma tissue cells. rod photoreceptor-like cells were also observed but mostly in a later stage of culture. neuronal differentiation does not require any additives suc ... | 2017 | 28866055 |
| genomic characteristics ofbifidobacterium thermacidophilumpig isolates and wild boar isolates reveal the unique presence of a putative mobile genetic element withtetwfor pig farm isolates. | genomic analysis was performed on seven strains ofbifidobacterium thermacidophilum, asus-associatedbifidobacterium. three strains from the feces of domestic pigs (sus scrofa domesticus) and four strains from the rectal feces of free-range japanese wild boars (s. s. scrofa) were compared. the phylogenetic position of these isolates suggested by genomic analyses were not concordant with that suggested by 16s rrna sequence. there was biased distribution of genes for virulence, phage, metabolism of ... | 2017 | 28861055 |
| corrigendum. | sayler ka, bigelow t, koster lg, swenson s, bounds c, hernández f, wisely sm. development of a rapid, simple, and specific real-time pcr assay for detection of pseudorabies viral dna in domestic swine herds. j vet diagn invest 2017;29:522-528. (original doi:10.1177/1040638717706593). in the article titled "development of a rapid, simple, and specific real-time pcr assay for detection of pseudorabies viral dna in domestic swine herds" by katherine a. sayler et al., the acknowledgements section, s ... | 2017 | 28859602 |
| synthesis, radiofluorination, and preliminary evaluation of the potential 5-ht2areceptor agonists [18f]cimbi-92 and [18f]cimbi-150. | an agonist pet tracer is of key interest for the imaging of the 5-ht2areceptor, as exemplified by the previously reported success of [11c]cimbi-36. fluorine-18 holds several advantages over carbon-11, making it the radionuclide of choice for clinical purposes. in this respect, an18f-labelled agonist 5-ht2areceptor (5-ht2ar) tracer is highly sought after. herein, we report a 2-step, 1-pot labelling methodology of 2 tracer candidates. both ligands display high in vitro affinities for the 5-ht2ar. ... | 2017 | 28856700 |
| n(pro) his49 and e(rns) lys412 mutations in pig bovine viral diarrhea virus type 2 synergistically enhance the cellular antiviral response. | type i interferons are major components of the innate immune response of hosts, and accordingly, many viruses have evolved mechanisms to modulate the host response during infection. bovine viral diarrhea virus (bvdv) nonstructural protein n(pro) and structural protein e(rns) play important roles in inhibiting type i interferon. the aim of this study was to explore the epistatic effects of amino acid mutations in n(pro) and e(rns) in porcine st cells to characterize the immune response induced by ... | 2017 | 28852929 |
| investigations of pro- and anti-apoptotic factors affecting african swine fever virus replication and pathogenesis. | african swine fever virus (asfv) is a large dna virus that replicates predominantly in the cell cytoplasm and is the only member of the asfarviridae family. the virus causes an acute haemorrhagic fever, african swine fever (asf), in domestic pigs and wild boar resulting in the death of most infected animals. apoptosis is induced at an early stage during virus entry or uncoating. however, asfv encodes anti-apoptotic proteins which facilitate production of progeny virions. these anti-apoptotic pro ... | 2017 | 28841179 |