Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| brassinosteroids act as a positive regulator for resistance against root knot nematode involving respiratory burst oxidase homolog-dependent activation of mapks in tomato. | interplay of hormones with reactive oxygen species (ros) fine-tunes the response of plants to stress, however, the crosstalk between brassinosteroids (brs) and ros in nematode resistance is unclear. in this study, we found that low br biosynthesis or lack of br receptor increased whilst exogenous br decreased the susceptibility of tomato plants to meloidogyne incognita. hormone quantification coupled with hormone mutant complementation experiments revealed that br did not induce the defense resp ... | 2017 | 28370079 |
| new nematotoxic indoloditerpenoid produced by gymnoascus reessii za-130. | chemical investigation of the fungal strain gymnoascus reessii za-130, which was previously isolated from the rhizosphere of tomato plants infected by the root-knot nematode meloidogyne incognita, led to the isolation and identification of a new indoloditerpenoid metabolite designated gymnoascole acetate. its structure was established by spectroscopic methods including 1d- and 2d-nmr and ms analyses. gymnoascole acetate demonstrated strong adverse effects on m. incognita second-stage juvenile (j ... | 2017 | 28343383 |
| histopathology combined with transcriptome analyses reveals the mechanism of resistance to meloidogyne incognita in cucumis metuliferus. | root-knot nematodes (meloidogyne spp.) cause serious threat to cucumber production. cucumis metuliferus, a relative of cucumber, is reported to be resistant to meloidogyne incognita, yet the underlying resistance mechanism remains unclear. in this study, the response of resistant c. metuliferus accession pi482443 following nematode infection was studied in comparison with susceptible c. sativus cv. jinlv no.3. roots of selected cucumis seedings were analysed using histological and biochemical te ... | 2017 | 28314173 |
| a glycine max homolog of non-race specific disease resistance 1 (ndr1) alters defense gene expression while functioning during a resistance response to different root pathogens in different genetic backgrounds. | a glycine max homolog of the arabidopsis thaliana non-race specific disease resistance 1 (ndr1) coiled-coil nucleotide binding leucine rich repeat (cc-nb-lrr) defense signaling gene (gm-ndr1-1) is expressed in root cells undergoing a defense response to the root pathogenic nematode, heterodera glycines. gm-ndr1-1 overexpression in the h. glycines-susceptible genotype g. max[williams 82/pi 518671] impairs parasitism. in contrast, gm-ndr1-1 rna interference (rnai) in the h. glycines-resistant geno ... | 2017 | 28273511 |
| genome sequence of delftia acidovorans hk171, a nematicidal bacterium isolated from tomato roots. | delftia acidovorans strain hk171, isolated from tomato roots, exhibited nematicidal activity against meloidogyne incognita here, we present the genome sequence of d. acidovorans strain hk171, which consists of one circular chromosome of 6,430,384 bp, with 66.9% g+c content. | 2017 | 28254991 |
| enhanced synthesis of antioxidant enzymes, defense proteins and leghemoglobin in rhizobium-free cowpea roots after challenging with meloydogine incognita. | the root knot nematodes (rkn), meloydogine spp., particularly meloidogyne incognita and meloidogyne javanica species, parasitize several plant species and are responsible for large annual yield losses all over the world. only a few available chemical nematicides are still authorized for rkn control owing to environmental and health reasons. thus, plant resistance is currently considered the method of choice for controlling rkn, and research performed on the molecular interactions between plants ... | 2014 | 28250394 |
| nematode neuropeptides as transgenic nematicides. | plant parasitic nematodes (ppns) seriously threaten global food security. conventionally an integrated approach to ppn management has relied heavily on carbamate, organophosphate and fumigant nematicides which are now being withdrawn over environmental health and safety concerns. this progressive withdrawal has left a significant shortcoming in our ability to manage these economically important parasites, and highlights the need for novel and robust control methods. nematodes can assimilate exog ... | 2017 | 28241060 |
| induction of sa-signaling pathway and ethylene biosynthesis in trichoderma harzianum-treated tomato plants after infection of the root-knot nematode meloidogyne incognita. | salicylic acid-signaling pathway and ethylene biosynthesis were induced in tomato treated with trichoderma harzianum when infected by root-knot nematodes and limited the infection by activation of sar and ethylene production. soil pre-treatment with trichoderma harzianum (th) strains item 908 (t908) and t908-5 decreased susceptibility of tomato to meloidogyne incognita, as assessed by restriction in nematode reproduction and development. the effect of t. harzianum treatments on plant defense was ... | 2017 | 28239746 |
| expression and evolutionary analyses of three acetylcholinesterase genes (mi-ace-1, mi-ace-2, mi-ace-3) in the root-knot nematode meloidogyne incognita. | the full cdna of mi-ace-3 encoding an acetylcholinesterase (ache) in meloidogyne incognita was cloned and characterized. mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. key residues essential to ache structure and function were conserved. the deduced mi-ace-3 protein sequence had 72% amino acid similarity with that of ditylenchus destructor dd-ache-3. phylogenetic analyses using 41 aches from 24 species showed that mi-ace-3 formed a cluster with 4 other nematode a ... | 2017 | 28238686 |
| transcriptome analysis of root-knot nematode (meloidogyne incognita)-infected tomato (solanum lycopersicum) roots reveals complex gene expression profiles and metabolic networks of both host and nematode during susceptible and resistance responses. | root knot nematodes (rkns, meloidogyne incognita) are economically important endoparasites having a wide-host range. we have taken a comprehensive transcriptomic approach to investigate the expression of both tomato and rkn genes in tomato roots at five infection time intervals from susceptible plants and two infection time intervals from resistant plants, grown under soil conditions. differentially expressed genes during susceptible (1827-tomato, 462-rkn) and resistance (25-tomato, 160-rkn) int ... | 2017 | 28220591 |
| three-dimensional ultrastructure of feeding tubes and interconnected endoplasmic reticulum in root-knot nematode-induced giant cells in rose balsam. | we investigated the three-dimensional ultrastructure of feeding tubes and the surrounding region in giant cells induced in rose balsam (impatiens balsamina l.) roots by the root-knot nematode meloidogyne incognita, using osmium maceration coupled with field emission scanning electron microscopy (fe-sem). in the roots of 35-day-old galled rose balsam plants, adult nematodes induced the formation of giant cells containing feeding tubes and numerous organelles, including tubular endoplasmic reticul ... | 2017 | 28204899 |
| genome-wide analysis of excretory/secretory proteins in root-knot nematode, meloidogyne incognita provides potential targets for parasite control. | the root-knot nematode, meloidogyne incognita causes significant damage to various economically important crops. infection is associated with secretion of effector proteins into host cytoplasm and interference with host innate immunity. to combat this infection, the identification and functional annotations of excretory/secretory (es) proteins serve as a key to produce durable control measures. the identification of es proteins through experimental methods are expensive and time consuming while ... | 2017 | 28187376 |
| sequential above- and belowground herbivory modifies plant responses depending on herbivore identity. | herbivore-induced changes in plant traits can cause indirect interactions between spatially and/or temporally separated herbivores that share the same host plant. feeding modes of the herbivores is one of the major factors that influence the outcome of such interactions. here, we tested whether the effects of transient aboveground herbivory for seven days by herbivores of different feeding guilds on tomato plants (solanum lycopersicum) alters their interaction with spatially as well as temporall ... | 2017 | 28178961 |
| phytoalexin phenalenone derivatives inactivate mosquito larvae and root-knot nematode as type-ii photosensitizer. | phytoalexins phenalenones (pns) are phytochemicals biosynthesized inside the plant in responsive to exterior threat. pns are excellent type-ii photosensitizers, which efficiently produce singlet oxygen upon light irradiation. based on the core functional structure of pns, novel pn derivatives were synthesized here and their singlet oxygen generating abilities and their phototoxicity were evaluated. at the presence of light, these pns have photoinduced toxicity towards aedes albopictus larvae and ... | 2017 | 28169356 |
| effects of soil textures on infectivity of root-knot nematodes on carrot. | this study was conducted to examine infectivity (penetration and gall and egg-mass formations) of the root-knot nematodes, meloidogyne incognita and m. hapla, on carrots grown in soil conditions of 5 different soil textures consisting of bed-soil (b) and sand (s) mixtures (b-s mixtures) at the ratios of 10:0, 7:3, 5:5, 3:7, and 0:10. for m. incognita, the nematode penetration rates in b-s of 0:10 (100% sand) were significantly higher than in the other b-s mixtures, more greatly at 2 and 5 days a ... | 2017 | 28167889 |
| microbial modulation of bacoside a biosynthetic pathway and systemic defense mechanism in bacopa monnieri under meloidogyne incognita stress. | plant-associated beneficial microbes have been explored to fulfill the imperative function for plant health. however, their impact on the host secondary metabolite production and nematode disease management remains elusive. our present work has shown that chitinolytic microbes viz., chitiniphilus sp. mtn22 and streptomyces sp. mtn14 singly as well as in combination modulated the biosynthetic pathway of bacoside a and systemic defense mechanism against meloidogyne incognita in bacopa monnieri. in ... | 2017 | 28157221 |
| acetic acid, 2-undecanone, and (e)-2-decenal ultrastructural malformations on meloidogyne incognita. | the use of natural compounds to control phytonematodes is significantly increasing, as most of the old synthetic pesticides have been banned due to their eco-hostile character. plant secondary metabolites are now evaluated as biologically active molecules against meloidogyne spp. but their target site in the nematode body is rarely specified. herein, we report on the ultrastructure modifications of the meloidogyne incognita j2 after treatment with nematicidal plant secondary metabolites, that is ... | 2016 | 28154431 |
| grafting and paladin pic-21 for nematode and weed management in vegetable production. | two years of field trials conducted in a meloidogyne incognita-infested field evaluated grafting and paladin pic-21 (dimethyl disulfide:chloropicrin [dmds:pic] 79:21) for root-knot nematode and weed control in tomato and melon. tomato rootstocks evaluated were; 'tx301', 'multifort', and 'aloha'. 'florida 47' was the scion and the nongrafted control. a double crop of melon was planted into existing beds following tomato harvest. melon rootstocks, c. metulifer and 'tetsukabuto', were evaluated wit ... | 2016 | 28154429 |
| gene expression analysis in musa acuminata during compatible interactions with meloidogyne incognita. | endoparasitic root-knot nematodes (rkns) (meloidogyne spp.) cause considerable losses in banana (musa spp.), with meloidogyne incognita a predominant species in cavendish sub-group bananas. this study investigates the root transcriptome in musa acuminata genotypes 4297-06 (aa) and cavendish grande naine (cav; aaa) during early compatible interactions with m. incognita methods: roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological ... | 2017 | 28130221 |
| single basal application of thiacloprid for the integrated management of meloidogyne incognita and bemisia tabaci in tomato crops. | tomato growers commonly face heavy nematode (meloidogyne incognita) and whitefly (b-biotype bemisia tabaci) infestations, and previous studies demonstrated that thiacloprid could be used to control m. incognita and b. tabaci in cucumber. however, the efficacy of a single basal application of thiacloprid to control both pests and its effect on yield in tomato remains unknown. in this study, the potential of thiacloprid application to the soil for the integrated control of m. incognita and b. taba ... | 2017 | 28120937 |
| exploiting cell cycle inhibitor genes of the krp family to control root-knot nematode induced feeding sites in plants. | cell cycle control in galls provoked by root-knot nematodes involves the activity of inhibitor genes like the arabidopsis ick/krp members. ectopic krp1, krp2 and krp4 expression resulted in decreased gall size by inhibiting mitotic activity, whereas krp6 induces mitosis in galls. herein, we investigate the role of krp3, krp5 and krp7 during gall development and compared their role with previously studied members of this class of cell cycle inhibitors. overexpression of krp3 and krp7 culminated i ... | 2017 | 28103637 |
| genetic and biochemical characterization of a gene operon for trans-aconitic acid, a novel nematicide from bacillus thuringiensis. | trans-aconitic acid (taa) is an isomer of cis-aconitic acid (caa), an intermediate of the tricarboxylic acid cycle that is synthesized by aconitase. although taa production has been detected in bacteria and plants for many years and is known to be a potent inhibitor of aconitase, its biosynthetic origins and the physiological relevance of its activity have remained unclear. we have serendipitously uncovered key information relevant to both of these questions. specifically, in a search for novel ... | 2017 | 28087696 |
| nematode root herbivory in tomato increases leaf defenses and reduces leaf miner oviposition and performance. | the outcome of plant-mediated interactions among herbivores from several feeding guilds has been studied intensively. however, our understanding on the effects of nematode root herbivory on leaf miner oviposition behavior and performance remain limited. in this study, we evaluated whether meloidogyne incognita root herbivory affects tuta absoluta oviposition preference on solanum lycopersicum plants and the development of the resulting offspring. to investigate the m. incognita-herbivory induced ... | 2017 | 28084560 |
| embryogenesis in the parasitic nematode heterodera glycines is independent of host-derived hatching stimulation. | many parasites regulate their development to synchronize their life cycle with a compatible host. the parasitic nematode heterodera glycines displays incomplete host-mediated hatching behavior wherein some h. glycines individuals hatch only in the presence of a host-derived cue while others hatch in water alone. furthermore, h. glycines shows variable hatching behavior based on oviposition location. the mechanisms regulating this hatching variability are unknown. in this study, we established a ... | 2017 | 28077087 |
| comparative efficacy of different approaches to managing meloidogyne incognita on green bean. | a greenhouse study was conducted to compare the relative efficacy of different approaches to managing meloidogyne incognita on green bean. these approaches included chemical (fumigant, non-fumigant, seed dressing, and seed dip), biological (the egg-parasitic fungus, paecilomyces lilacinus and the mycorrhizal fungus glomus sp.), physical (soil solarization), and cultural (chicken litter and urea) methods. accordingly, nine different control materials and application methods plus nematode-infected ... | 2017 | 28053585 |
| nematicidal activity of trans-2-hexenal against southern root-knot nematode (meloidogyne incognita) on tomato plants. | botanical nematicides have recently received increasing interest because of the high risks of some traditional nematicides to human health and the environment. this study evaluated the nematicidal activity of a plant volatile, trans-2-hexenal, against meloidogyne incognita. this compound exhibited higher activity in a fumigation experiment than in the aqueous phase in vitro. both in pot tests and in field trials, trans-2-hexenal showed significant efficacy against m. incognita while maintaining ... | 2017 | 28048941 |
| a whole-plant perspective reveals unexpected impacts of above- and belowground herbivores on plant growth and defense. | trade-offs between plant growth and defense are central to theoretical frameworks used to study the ecology and evolution of plant defense against herbivores. however, these frameworks, as well as the experiments designed to test them, rarely include belowground herbivores. we experimentally challenged seedlings of the tropical shrub solanum lycocarpum (solanaceae) with either aboveground foliar herbivores (spodoptera caterpillars) or belowground root herbivores (the nematode meloidogyne incogni ... | 2017 | 27935027 |
| transcriptome analysis of resistant and susceptible tobacco (nicotiana tabacum) in response to root-knot nematode meloidogyne incognita infection. | the root-knot nematode (rkn) meloidogyne incognita reproduces on the roots of tobacco (nicotiana tabacum), damaging crops, reducing crop yield, and causing economic losses annually. the development of resistant genotypes is an alternative strategy to effectively control these losses. however, the molecular mechanism responsible for host pathogenesis and defense responses in tobacco specifically against rkns remain poorly understood. here, root transcriptome analysis of resistant (yuyan12) and su ... | 2017 | 27914810 |
| mutations in acetylcholinesterase2 (ace2) increase the insensitivity of acetylcholinesterase to fosthiazate in the root-knot nematode meloidogyne incognita. | the root-knot nematode meloidogyne incognita causes severe damage to continuously cropping vegetables. the control of this nematode relies heavily on organophosphate nematicides in china. here, we described resistance to the organophosphate nematicide fosthiazate in a greenhouse-collected resistant population (rp) and a laboratory susceptible population (sp) of m. incognita. fosthiazate was 2.74-fold less toxic to nematodes from rp than that from sp. quantitative real-time pcr revealed that the ... | 2016 | 27897265 |
| resistance-breaking population of meloidogyne incognita utilizes plant peroxidase to scavenge reactive oxygen species, thereby promoting parasitism on tomato carrying mi-1 gene. | resistance conferred by the mi-1 gene from solanum peruvianum is effective and widely used for controlling root-knot nematodes (rkns, meloidogyne spp.). however, breakdown of resistance by rkns seriously threatens the durable application of the resistance resource. here, a resistance-breaking population of m. incognita was selected from an avirulent population by continuously inoculating on mi-1-carrying tomato. histological observations showed the resistance-breaking population would not induce ... | 2017 | 27836538 |
| shifting from priming of salicylic acid- to jasmonic acid-regulated defences by trichoderma protects tomato against the root knot nematode meloidogyne incognita. | beneficial root endophytes such as trichoderma spp. can reduce infections by parasitic nematodes through triggering host defences. little is currently known about the complex hormone signalling underlying the induction of resistance. in this study, we investigated whether trichoderma modulates the hormone signalling network in the host to induce resistance to nematodes. we investigated the role and the timing of the jasmonic acid (ja)- and salicylic acid (sa)-regulated defensive pathways in tric ... | 2017 | 27801946 |
| advancements in breeding, genetics, and genomics for resistance to three nematode species in soybean. | integration of genetic analysis, molecular biology, and genomic approaches drastically enhanced our understanding of genetic control of nematode resistance and provided effective breeding strategies in soybeans. three nematode species, including soybean cyst (scn, heterodera glycine), root-knot (rkn, meloidogyne incognita), and reniform (rn, rotylenchulus reniformis), are the most destructive pests and have spread to soybean growing areas worldwide. host plant resistance has played an important ... | 2016 | 27796432 |
| stage-wise identification and analysis of mirna from root-knot nematode meloidogyne incognita. | in this study, we investigated global changes in mirnas of meloidogyne incognita throughout its life cycle. small rna sequencing resulted in approximately 62, 38, 38, 35, and 39 mb reads in the egg, j2, j3, j4, and female stages, respectively. overall, we identified 2724 known and 383 novel mirnas (read count > 10) from all stages, of which 169 known and 13 novel mirna were common to all the five stages. among the stage-specific mirnas, mir-286 was highly expressed in eggs, mir-2401 in j2, mir-8 ... | 2016 | 27775666 |
| mitochondrial haplotype-based identification of root-knot nematodes (meloidogyne spp.) on cut foliage crops in florida. | florida accounts for more than 75% of the national cut foliage production. unfortunately, root-knot nematodes (rkn) (meloidogyne spp.) are a serious problem on these crops, rendering many farms unproductive. currently, information on the meloidogyne spp. occurring on most commonly cultivated cut foliage crops in florida, and tools for their rapid identification are lacking. the objectives of this study were to (i) identify specific rkn infecting common ornamental cut foliage crops in florida and ... | 2016 | 27765993 |
| nuclear receptor nhr-48 is required for pathogenicity of the second stage (j2) of the plant parasite meloidogyne incognita. | nuclear receptors (nrs) are a diverse class of transcription factors, which are involved in regulating a large number of physiological events in metazoans. however, the function of nrs is poorly understood in plant-parasitic nematodes. here, members of the nr1j+k group of nrs in nematodes, including the free-living and plant parasites, were examined and phylogenetically analyzed. we found that the number of members of the nr1j+k group in plant-parasitic nematodes was less than that in the free-l ... | 2016 | 27762328 |
| nematicidal key precursors for the biosynthesis of morphological regulatory arthrosporols in nematode-trapping fungus arthrobotrys oligospora. | arthrobotrys oligospora is the first recognized nematode-trapping fungus and by far the most abundant in the environment. our recent study revealed the polyketide synthase (pks) gene aol_s00215g283 in a. oligospora involved in the production of many secondary metabolites and the trap formation of the fungus. here we report that the disruption of two genes in the upstream flanking region of the gene aol_s00215g283, aol_s00215g281 and aol_s00215g282, which putatively encoded one amidohydrolase and ... | 2016 | 27723963 |
| comparative analysis of soybean genotype resistance to heterodera glycines and meloidogyne species via resistance gene analogs. | nematodes are important pests of soybean throughout the world and cause high yield losses. as a control strategy, the identification of resistance genes is an important aim of breeding studies. plants possess resistance genes (r), which are responsible for the recognition of pathogens and activation of the defense system. r genes and resistance gene analogs (rgas) possess conserved domains, from which nucleotide-binding site is the most common. using degenerate primers originating from these dom ... | 2016 | 27706689 |
| bioprospecting for secondary metabolites in the entomopathogenic bacterium photorhabdus luminescens subsp. sonorensis. | crude extracts of in vitro and in vivo cultures of two strains of photorhabdus l. sonorensis (enterobacteriaceae) were analyzed by tlc, hplc-uv and lc-ms. nine unique compounds with mass/charge ratios (m/z) ranging from 331.3 to 713.5 were found in ms analyses. bioactivity of extracts was assessed on a selection of plant pathogens/pests and non-target species. caborca strain extracts showed the highest activity against helicoverpa zea (lepidoptera: noctuidae) neonates at all concentrations teste ... | 2016 | 27702563 |
| the infection of cucumber (cucumis sativus l.) roots by meloidogyne incognita alters the expression of actin-depolymerizing factor (adf) genes, particularly in association with giant cell formation. | cucumber (cucumis sativus l.) is threatened by substantial yield losses due to the south root-knot nematode (meloidogyne incognita). however, understanding of the molecular mechanisms underlying the process of nematode infection is still limited. in this study, we found that m. incognita infection affected the structure of cells in cucumber roots and treatment of the cytoskeleton inhibitor (cytochalasin d) reduced root-knot nematode (rkn) parasitism. it is known that actin-depolymerizing factor ... | 2016 | 27695469 |
| identification of nematicidal constituents of notopterygium incisum rhizomes against bursaphelenchus xylophilus and meloidogyne incognita. | during a screening program for new agrochemicals from chinese medicinal herbs, the ethanol extract of notopterygium incisum rhizomes was found to possess strong nematicidal activity against the two species of nematodes, bursaphelenchus xylophilus and meloidogyne incognita. based on bioactivity-guided fractionation, the four constituents were isolated from the ethanol extract and identified as columbianetin, falcarindiol, falcarinol, and isoimperatorin. among the four isolated constituents, two a ... | 2016 | 27669203 |
| evaluation of in vitro and in vivo nematicidal potential of a multifunctional streptomycete, streptomyces hydrogenans strain dh16 against meloidogyne incognita. | the present work demonstrated the nematicidal potential of streptomyces hydrogenans strain dh16 (a strain with strong antagonism against fungal phytopathogens and insect pest) against meloidogyne incognita. the culture supernatant and solvent extract significantly inhibited egg hatching (almost 100%) along with j2 mortality of more than 95% after 96h. the nematicidal activity of 10-(2,2-dimethyl-cyclohexyl)-6,9-dihydroxy-4,9-dimethyl-dec-2-enoic acid methyl ester (sh2; a new antifungal compound) ... | 2016 | 27664743 |
| direct nematicidal effects of methyl jasmonate and acibenzolar-s-methyl against meloidogyne incognita. | the aim of this study was to examine the nematicidal properties of two defence inducers against the root-knot nematode meloidogyne incognita. a direct-contact bioassay was applied to evaluate the nematicidal effects of acibenzolar-s-methyl (asm) and methyl jasmonate (meja) on second-stage juveniles (j2). nematodes were incubated in different concentrations of these compounds, and the numbers of immobile nematodes were counted after 24 and 48 h post incubation. tap water was then added to verify ... | 2017 | 27658537 |
| versatile glycoside hydrolase family 18 chitinases for fungi ingestion and reproduction in the pinewood nematode bursaphelenchus xylophilus. | the glycoside hydrolase family 18 (gh18) of chitinases is a gene family widely expressed in archaes, prokaryotes and eukaryotes, and hydrolyzes the β-1,4-linkages in chitin. the pinewood nematode bursaphelenchus xylophilus is one of the organisms that produces gh18 chitinases. notably, b. xylophilus has a higher number of gh18 chitinases compared with the obligate plant-parasitic nematodes meloidogyne incognita and meloidogyne hapla. in this study, seven gh18 chitinases were identified and clone ... | 2016 | 27641827 |
| expression of a cystatin transgene in eggplant provides resistance to root-knot nematode, meloidogyne incognita. | root-knot nematodes (rkn) cause substantial yield decline in eggplant and sustainable management options to minimize crop damage due to nematodes are still limited. a number of genetic engineering strategies have been developed to disrupt the successful plant-nematode interactions. among them, delivery of proteinase inhibitors from the plant to perturb nematode development and reproduction is arguably the most effective strategy. in the present study, transgenic eggplant expressing a modified ri ... | 2016 | 27516765 |
| fine mapping and identification of candidate genes for a qtl affecting meloidogyne incognita reproduction in upland cotton. | the southern root-knot nematode (meloidogyne incognita; rkn) is one of the most important economic pests of upland cotton (gossypium hirsutum l.). host plant resistance, the ability of a plant to suppress nematode reproduction, is the most economical, practical, and environmentally sound method to provide protection against this subterranean pest. the resistant line auburn 623rnr and a number of elite breeding lines derived from it remain the most important source of root-knot nematode (rkn) res ... | 2016 | 27503539 |
| chip technologies for screening chemical and biological agents against plant-parasitic nematodes. | plant-parasitic nematodes cause substantial damage to agricultural crops worldwide. long-term management of these pests requires novel strategies to reduce infection of host plants. disruption of nematode chemotaxis to root systems has been proposed as a potential management approach, and novel assays are needed to test the chemotactic behavior of nematodes against a wide range of synthetic chemicals and root exudates. two microfluidic chips were developed that measure the attraction or repulsio ... | 2016 | 27452899 |
| the fungal endophyte chaetomium globosum negatively affects both above- and belowground herbivores in cotton. | mutualistic plant-endophyte symbioses can benefit plants by increasing host fitness through reductions in herbivory. the fungus, chaetomium globosum strain tamu 520, was previously isolated as an endophyte from cotton (gossypium hirsutum) and can be re-inoculated to systemically colonize cotton plants via seed treatment. we evaluated the potential impacts of the endophyte in cotton on plant parasitic nematodes belowground, along with piercing-sucking and chewing insects aboveground. endophytic c ... | 2016 | 27451418 |
| synthesis and biological evaluation of 3,5-dimethoxystilbene analogs. | in our continuing effort to discover natural product-based pest management agents, derivatives of 3,5-dimethoxystilbene were synthesized yielding 27 new and six known compounds. compounds 11 and 12 showed strong aedes aegypti larvicidal activity (lc50 45.31 and 49.93 μm, respectively). furthermore, 11 and 12 exhibited high effectiveness against larvae of pesticide-susceptible and pyrethroid-resistant strains of ae. aegypti; activity against the adult mosquitoes was low. compounds 6f, 6g, and 6i ... | 2016 | 27450661 |
| a novel meloidogyne incognita effector misp12 suppresses plant defense response at latter stages of nematode parasitism. | secreted effectors in plant root-knot nematodes (rkns, or meloidogyne spp.) play key roles in their parasite processes. currently identified effectors mainly focus on the early stage of the nematode parasitism. there are only a few reports describing effectors that function in the latter stage. in this study, we identified a potential rkn effector gene, misp12, that functioned during the latter stage of parasitism. misp12 was unique in the meloidogyne spp., and highly conserved in meloidogyne in ... | 2016 | 27446188 |
| trichinella spiralis: adaptation and parasitism. | publication of the genome from the clade i organism, trichinella spiralis, has provided us an avenue to address more holistic problems in parasitology; namely the processes of adaptation and the evolution of parasitism. parasitism among nematodes has evolved in multiple, independent events. deciphering processes that drive species diversity and adaptation are keys to understanding parasitism and advancing control strategies. studies have been put forth on morphological and physiological aspects ... | 2016 | 27425574 |
| reproduction of meloidogyne incognita race 3 on flue-cured tobacco homozygous for rk1 and/or rk2 resistance genes. | most commercial tobacco cultivars possess the rk1 resistance gene to races 1 and 3 of meloidogyne incognita and race 1 of meloidogyne arenaria, which has caused a shift in population prevalence in virginia tobacco fields toward other species and races. a number of cultivars now also possess the rk2 gene for root-knot resistance. experiments were conducted in 2013 to 2014 to examine whether possessing both rk1 and rk2 increases resistance to a variant of m. incognita race 3 compared to either gen ... | 2016 | 27418700 |
| efficacy of various application methods of fluensulfone for managing root-knot nematodes in vegetables. | fluensulfone is a new nematicide in the flouroalkenyl chemical group. a field experiment was conducted in 2012 and 2013 to evaluate the efficacy of various application methods of fluensulfone for control of meloidogyne spp. in cucumber (cucumis sativus). treatments of fluensulfone (3.0 kg a.i./ha) were applied either as preplant incorporation (ppi) or via different drip irrigation methods: drip without pulse irrigation (drip np), pulse irrigation 1 hr after treatment (drip +1p), and treatment at ... | 2016 | 27418698 |
| effect of plant resistance and bioact wg (purpureocillium lilacinum strain 251) on meloidogyne incognita in a tomato-cucumber rotation in a greenhouse. | the effectiveness of combining resistant tomato with bioact wg (purpureocillium lilacinum strain 251, pl251) against meloidogyne incognita was assessed in a tomato-cucumber rotation in a greenhouse over 2 years. additionally, the enzymatic activity of the fungus, the percentage of fungal egg and juvenile parasitism, cardinal temperatures and the effect of water potential on mycelial growth and the soil receptivity to pl251 were determined in vitro. | 2017 | 27414251 |
| untargeted metabolomics of tomato plants after root-knot nematode infestation. | after 2 months from the infestation of tomato plants with the root-knot nematode (rkn) meloidogyne incognita, we performed a gas chromatography-mass spectrometry untargeted fingerprint analysis for the identification of characteristic metabolites and biomarkers. principal component analysis, and orthogonal projections to latent structures discriminant analysis suggested dramatic local changes of the plant metabolome. in the case of tomato leaves, β-alanine, phenylalanine, and melibiose were indu ... | 2016 | 27389052 |
| comparative genomic and functional analyses: unearthing the diversity and specificity of nematicidal factors in pseudomonas putida strain 1a00316. | we isolated pseudomonas putida (p. putida) strain 1a00316 from antarctica. this bacterium has a high efficiency against meloidogyne incognita (m. incognita) in vitro and under greenhouse conditions. the complete genome of p. putida 1a00316 was sequenced using pacbio single molecule real-time (smrt) technology. a comparative genomic analysis of 16 pseudomonas strains revealed that although p. putida 1a00316 belonged to p. putida, it was phenotypically more similar to nematicidal pseudomonas fluor ... | 2016 | 27384076 |
| bacillus cereus strain s2 shows high nematicidal activity against meloidogyne incognita by producing sphingosine. | plant-parasitic nematodes cause serious crop losses worldwidely. this study intended to discover the antagonistic mechanism of bacillus cereus strain s2 against meloidogyne incognita. treatment with b. cereus strain s2 resulted in a mortality of 77.89% to caenorhabditis elegans (a model organism) and 90.96% to m. incognita. in pot experiment, control efficiency of b. cereus s2 culture or supernatants were 81.36% and 67.42% towards m. incognita, respectively. in field experiment, control efficien ... | 2016 | 27338781 |
| coupling of mic-3 overexpression with the chromosomes 11 and 14 root-knot nematode (rkn) (meloidogyne incognita) resistance qtls provides insights into the regulation of the rkn resistance response in upland cotton (gossypium hirsutum). | genetic analysis of mic-3 transgene with rkn resistance qtls provides insight into the resistance regulatory mechanism and provides a framework for testing additional hypotheses. resistance to root-knot nematode (rkn) (meloidogyne incognita) in upland cotton (gossypium hirsutum) is mediated by two major quantitative trait loci (qtl) located on chromosomes 11 and 14. the mic-3 (meloidogyne induced cotton3) protein accumulates specifically within the immature galls of rkn-resistant plants that pos ... | 2016 | 27314265 |
| synthesis, nematocidal activity and docking study of novel chiral 1-(3-chloropyridin-2-yl)-3-(trifluoromethyl)-1h-pyrazole-4-carboxamide derivatives. | a series of novel chiral fluorinated pyrazole carboxamides derivatives were designed and synthesized. all these title compounds were confirmed by nmr and ms. the primarily nematocidal activity results indicated that some of them exhibited good control efficacy against the tomato root-knot nematode disease caused by meloidogyne incognita. the docking results indicated that compound 5n interact with amino acid residue tyr 121, trp 279 of ache via hydrogen bond. | 2016 | 27301365 |
| influence of root-knot nematode infestation on antioxidant enzymes, chlorophyll content and growth in pogostemon cablin (blanco) benth. | plants adapt themselves to overcome adverse environmental conditions, and this involves a plethora of concurrent cellular activities. physiological experiments or metabolic profiling can quantify this response. among several diseases of pogostemon cablin (blanco) benth. (patchouli), root-knot nematode infection caused by meloidogyne incognita (kofoid and white) chitwood causes severe damage to the plant and hence, the oil production. in the present study, we identified m. incognita morphological ... | 2016 | 27295922 |
| biological control of meloidogyne incognita by aspergillus niger f22 producing oxalic acid. | restricted usage of chemical nematicides has led to development of environmentally safe alternatives. a culture filtrate of aspergillus niger f22 was highly active against meloidogyne incognita with marked mortality of second-stage juveniles (j2s) and inhibition of egg hatching. the nematicidal component was identified as oxalic acid by organic acid analysis and gas chromatography-mass spectroscopy (gc-ms). exposure to 2 mmol/l oxalic acid resulted in 100% juvenile mortality at 1 day after treat ... | 2016 | 27258452 |
| potent nematicidal activity of maleimide derivatives on meloidogyne incognita. | different maleimide derivatives were synthesized and assayed for their in vitro activity on the soil inhabiting, plant-parasitic nematode meloidogyne incognita, also known as root-knot nematode. the compounds maleimide, n-ethylmaleimide, n-isopropylmaleimide, and n-isobutylmaleimide showed the strongest nematicidal activity on the second stage juveniles of the root-knot nematode with ec50/72h values of 2.6 ± 1.3, 5.1 ± 3.4, 16.2 ± 5.4, and 19.0 ± 9.0 mg/l, respectively. we also determined the ne ... | 2016 | 27249054 |
| plant genetic background increasing the efficiency and durability of major resistance genes to root-knot nematodes can be resolved into a few resistance qtls. | with the banning of most chemical nematicides, the control of root-knot nematodes (rkns) in vegetable crops is now based essentially on the deployment of single, major resistance genes (r-genes). however, these genes are rare and their efficacy is threatened by the capacity of rkns to adapt. in pepper, several dominant r-genes are effective against rkns, and their efficacy and durability have been shown to be greater in a partially resistant genetic background. however, the genetic determinants ... | 2016 | 27242835 |
| [efficiency of trichoderma longibrachiatum t6 in the control of meloidogyne incognita and its rhizosphere colonization in cucumber]. | efficiency of different concentrations of trichoderma longibrachiatum t6 against meloidogyne incognita and its rhizosphere colonization in cucumber were determined in greenhouse experiments. the results of rhizosphere colonization experiments showed that the number of colonies in cucumber soil and root increased significantly ten weeks after inoculation with the second stage juveniles of m. incognita and different concentrations of t. longibrachiatum t6, and there was significant difference in d ... | 2016 | 27228616 |
| nematicidal activity of kojic acid produced by aspergillus oryzae against meloidogyne incognita. | the fungal strain eml-dml3pna1 isolated from leaf of white dogwood (cornus alba l.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. the active fungal strain was identified as aspergillus oryzae, which belongs to section flavi, based on the morphological characteristics and sequence analysis of the its rdna, calmodulin (cam), and β-tubulin (bena) genes. the strain reduced the ph value to 5.62 afte ... | 2016 | 27197670 |
| assessment of dapg-producing pseudomonas fluorescens for management of meloidogyne incognita and fusarium oxysporum on watermelon. | pseudomonas fluorescens isolates clinto 1r, wayne 1r, and wood 1r, which produce the antibiotic 2,4-diacetylphloroglucinol (dapg), can suppress soilborne diseases and promote plant growth. consequently, these beneficial bacterial isolates were tested on watermelon plants for suppression of meloidogyne incognita (root-knot nematode: rkn) and fusarium oxysporum f. sp. niveum (fon). in a greenhouse trial, wayne 1r root dip suppressed numbers of rkn eggs per gram root on 'charleston gray' watermelon ... | 2016 | 27168652 |
| resistance to southern root-knot nematode (meloidogyne incognita) in wild watermelon (citrullus lanatus var. citroides). | southern root-knot nematode (rkn, meloidogyne incognita) is a serious pest of cultivated watermelon (citrullus lanatus var. lanatus) in southern regions of the united states and no resistance is known to exist in commercial watermelon cultivars. wild watermelon relatives (citrullus lanatus var. citroides) have been shown in greenhouse studies to possess varying degrees of resistance to rkn species. experiments were conducted over 2 yr to assess resistance of southern rkn in c. lanatus var. citro ... | 2016 | 27168648 |
| optimization of in vitro techniques for distinguishing between live and dead second stage juveniles of heterodera glycines and meloidogyne incognita. | heterodera glycines (soybean cyst nematode, or scn) and meloidogyne incognita (root-knot nematode, or rkn) are two damaging plant-parasitic nematodes on important field crops. developing a quick method to distinguish between live and dead scn and rkn second stage juveniles (j2) is vital for high throughput screening of pesticides or biological compounds against scn and rkn. the in vitro assays were conducted in 96-well plates to determine the optimum chemical stimulus to distinguish between live ... | 2016 | 27144277 |
| poplar-root knot nematode interaction: a model for perennial woody species. | plant root-knot nematode (rkn) interaction studies are performed on several host plant models. though rkn interact with trees, no perennial woody model has been explored so far. here, we show that poplar (populus tremula × p. alba) grown in vitro is susceptible to meloidogyne incognita, allowing this nematode to penetrate, to induce feeding sites, and to successfully complete its life cycle. quantitative reverse transcription-polymerase chain reaction analysis was performed to study changes in p ... | 2016 | 27135257 |
| impact of direct and indirect application of rising furfural concentrations on viability, infectivity and reproduction of the root-knot nematode, meloidogyne incognita in pisum sativum. | the gradual withdraw of several broadly used nematicides from market has enhanced the need to develop sustainable and eco-friendly alternatives with nematicidal properties. furfural is one of the promising alternatives to fill this need. baseline information about the impact of furfural on egg hatch, penetration potential and ultrastructure of nematode is lacking. in this study, the reagent-grade (purity ≥ 99.0%) of furfural was applied against meloidogyne incognita. in vitro tests showed gradua ... | 2016 | 27133267 |
| effects of tomato root exudates on meloidogyne incognita. | plant root exudates affect root-knot nematodes egg hatch. chemicals in root exudates can attract nematodes to the roots or result in repellence, motility inhibition or even death. however, until recently little was known about the relationship between tomato root exudates chemicals and root-knot nematodes. in this study, root exudates were extracted from three tomato rootstocks with varying levels of nematode resistance: baliya (highly resistant, hr), rs2 (moderately resistant, mr) and l-402 (hi ... | 2016 | 27128659 |
| genomes of parasitic nematodes (meloidogyne hapla, meloidogyne incognita, ascaris suum and brugia malayi) have a reduced complement of small rna interference pathway genes: knockdown can reduce host infectivity of m. incognita. | the discovery of rna interference (rnai) as an endogenous mechanism of gene regulation in a range of eukaryotes has resulted in its extensive use as a tool for functional genomic studies. it is important to study the mechanisms which underlie this phenomenon in different organisms, and in particular to understand details of the effectors that modulate its effectiveness. the aim of this study was to identify and compare genomic sequences encoding genes involved in the rnai pathway of four parasit ... | 2016 | 27126863 |
| early development of the root-knot nematode meloidogyne incognita. | detailed descriptions of the early development of parasitic nematodes are seldom available. the embryonic development of the plant-parasitic nematode meloidogyne incognita was studied, focusing on the early events. | 2016 | 27122249 |
| a novel serine protease, sep1, from bacillus firmus ds-1 has nematicidal activity and degrades multiple intestinal-associated nematode proteins. | plant-parasitic nematodes (ppns) cause serious harm to agricultural production. bacillus firmus shows excellent control of ppns and has been produced as a commercial nematicide. however, its nematicidal factors and mechanisms are still unknown. in this study, we showed that b. firmus strain ds-1 has high toxicity against meloidogyne incognita and soybean cyst nematode. we sequenced the whole genome of ds-1 and identified multiple potential virulence factors. we then focused on a peptidase s8 sup ... | 2016 | 27118554 |
| ozonated water reduces susceptibility in tomato plants to meloidogyne incognita by the modulation of the antioxidant system. | few studies have been carried out on the effect of ozonated water (o3 wat) on the oxidative stress of root systems and, in particular, in combination with biotic stress. the aim of this study was to determine whether aqueous ozone is effective in the control of root-knot nematode (rkn) infection and to investigate the concomitant changes in the basal defence system. a tomato cultivar susceptible to meloidogyne incognita was treated with o3 wat as a soil drench. no negative effects were seen foll ... | 2017 | 27071373 |
| evidence for the involvement of nematocidal toxins of purpureocillium lilacinum 6029 cultured on karanja deoiled cake liquid medium. | in present study, in vitro nematocidal bioassays, ft-ir and hplc analysis were employed to demonstrate the involvement of toxins of purpureocillium lilacinum in killing root-knot nematodes (meloidogyne incognita). during growth study, maximum mycelial biomass (10.52 g/l) in de-oiled karanja cake medium was achieved on 8th day while complete mortality of nematodes was obtained by 6th day filtrate (fksm). maximum production of crude nematocidal toxin was recorded on 7th day suggesting that the tox ... | 2016 | 27038952 |
| exogenous rna interference exposes contrasting roles for sugar exudation in host-finding by plant pathogens. | plant parasitic nematodes (ppn) locate host plants by following concentration gradients of root exudate chemicals in the soil. we present a simple method for rna interference (rnai)-induced knockdown of genes in tomato seedling roots, facilitating the study of root exudate composition, and ppn responses. knockdown of sugar transporter genes, stp1 and stp2, in tomato seedlings triggered corresponding reductions of glucose and fructose, but not xylose, in collected root exudate. this corresponded ... | 2016 | 27033013 |
| sensitivity of meloidogyne incognita and rotylenchulus reniformis to fluopyram. | fluopyram is a succinate dehydrogenase inhibitor (sdhi) fungicide that is being evaluated as a seed treatment and in-furrow spray at planting on row crops for management of fungal diseases and its effect on plant-parasitic nematodes. currently, there are no data on nematode toxicity, nematode recovery, or effects on nematode infection for meloidogyne incognita or rotylenchulus reniformis after exposure to low concentrations of fluopyram. nematode toxicity and recovery experiments were conducted ... | 2015 | 26941460 |
| root-knot nematodes induce pattern-triggered immunity in arabidopsis thaliana roots. | root-knot nematodes (rkns; meloidogyne spp.) are plant parasites with a broad host range causing great losses worldwide. to parasitize their hosts, rkns establish feeding sites in roots known as giant cells. the majority of work studying plant-rkn interactions in susceptible hosts addresses establishment of the giant cells and there is limited information on the early defense responses. here we characterized early defense or pattern-triggered immunity (pti) against rkns in arabidopsis thaliana. ... | 2016 | 26892116 |
| analysis of root-knot nematode and fusarium wilt disease resistance in cotton (gossypium spp.) using chromosome substitution lines from two alien species. | chromosome substitution (cs) lines in plants are a powerful genetic resource for analyzing the contribution of chromosome segments to phenotypic variance. in this study, a series of interspecific cotton (gossypium spp.) cs lines were used to identify a new germplasm resource, and to validate chromosomal regions and favorable alleles associated with nematode or fungal disease resistance traits. the cs lines were developed in the g. hirsutum l. tm-1 background with chromosome or chromosome segment ... | 2016 | 26882892 |
| isolation and identification of nematode-antagonistic compounds from the fungus aspergillus candidus. | culture medium from an isolate of the fungus aspergillus candidus was extracted, fractionated and examined to discover compounds antagonistic to plant-parasitic nematodes that are important pathogens of agricultural crops. column, thin layer and preparative chromatographies and spectral and elemental analyses, were used to isolate and identify two major constituents of an active fraction (fraction f) obtained from the medium. compound 1 was identified as 2-hydroxypropane-1, 2, 3-tricarboxylic ac ... | 2016 | 26850440 |
| alcaligenes faecalis zd02, a novel nematicidal bacterium with an extracellular serine protease virulence factor. | root knot nematodes (rkns) are the world's most damaging plant-parasitic nematodes (ppns), and they can infect almost all crops. at present, harmful chemical nematicides are applied to control rkns. using microbial nematicides has been proposed as a better management strategy than chemical control. in this study, we describe a novel nematicidal bacterium named alcaligenes faecalis zd02. a. faecalis zd02 was isolated from caenorhabditis elegans cadavers and has nematostatic and nematicidal activi ... | 2016 | 26826227 |
| msp40 effector of root-knot nematode manipulates plant immunity to facilitate parasitism. | root-knot nematodes (rkns) are obligate biotrophic parasites that invade plant roots and engage in prolonged and intimate relationships with their hosts. nematode secretions, some of which have immunosuppressing activity, play essential roles in successful parasitism; however, their mechanisms of action remain largely unknown. here, we show that the rkn-specific gene mimsp40, cloned from meloidogyne incognita, is expressed exclusively in subventral oesophageal gland cells and is strongly upregul ... | 2016 | 26797310 |
| mi-flp-18 and mi-mpk-1 genes are potential targets for meloidogyne incognita control. | meloidogyne incognita is a major plant parasite that causes root-knot disease in numerous agricultural crops. this nematode has severely affected greenhouse crops in china. chemical insecticides are generally used to control this pest, but they have adverse environmental and human toxicity effects; hence, safe and effective strategies for controlling the root-knot nematode (rkn) are necessary. fmrfamide-like peptides (flps) have diverse physiological and biological effects on the locomotory, fee ... | 2016 | 26785173 |
| resistance of upland-rice lines to root-knot nematode, meloidogyne incognita. | despite the benefits of crop rotation, occurrence of nematodes is a common problem for almost all crops within the cerrado biome, especially for rice. the use of resistant cultivars is one of the main methods for control of nematodes. thus, the present study aimed to evaluate the reaction of 36 upland-rice lines, with desirable agronomic characteristics, according to their resistance to root-knot nematodes (meloidogyne incognita). the experimental design was entirely randomized with four replica ... | 2015 | 26782379 |
| identification and characterization of micrornas in the plant parasitic root-knot nematode meloidogyne incognita using deep sequencing. | the root-knot nematode meloidogyne incognita is among the most damaging plant-parasitic pests of several crops including cotton (gossypium hirsutum) and tomato (lycopersicon escultentum). recently, a genome has become available for m. incognita, which greatly facilitates investigation of the interactions between m. incognita and its plant hosts at the molecular level and enables formation of hypotheses concerning development at the cellular level. micrornas (mirnas) are a class of small rna mole ... | 2016 | 26743520 |
| development and reproductive potential of tyrophagus putrescentiae (acari: acaridae) on plant-parasitic nematodes and artificial diets. | this study investigated development, reproduction and life table parameters of the astigmatid mold mite tyrophagus putrescentiae (schrank) (acari: acaridae) feeding on egg-masses or adult females of the nematode meloidogyne incognita, egg-masses of the nematode rotylenchulus reniformis, ras cheese or yeast at 25 ± 1 °c, 70 ± 10 % rh in the dark. immature developmental times were shorter when the mite was fed females of m. incognita followed by yeast. different prey/diet types had no significant ... | 2016 | 26692383 |
| [eupolyphaga frass and its extracts protected tomato from meloidogyne incognita infestation]. | the control effects of eupolyphaga (eupolyphaga sinensis walker) frass and its extracts on meloidogyne incognita were studied through laboratory assays and pot experiments, and the organic volatile compounds and oligochitosan contents in the frass were analyzed. the results indicated that the nematode immobility and mortality was significantly increased with increasing the extract concentration and treatment time. compared with the control, egg hatching was significantly inhibited when the extra ... | 2015 | 26685616 |
| in vivo imaging of microtubule organization in dividing giant cell. | mitosis which is a major step during plant development can also be observed in physiopathological conditions. during the compatible interaction between the root-knot nematode meloidogyne incognita and its host arabidopsis, the pathogen induce through repeated divisions without complete cytokinesis the formation of hypertrophied and multinucleate feeding cells, named giant cells. due to the presence of hypertrophied plant cell material surrounding the giant cells, classical live cell imaging gave ... | 2016 | 26659960 |
| the complete genome sequence of alcaligenes faecalis zd02, a novel potential bionematocide. | root-knot nematodes (rkns) can infect almost all crops, and result in huge economic losses in agriculture. there is no effective and environmentally safe means available to control rkns. alcaligenes faecalis zd02 isolated from free living nematode caenorhabditis elegans cadavers shows toxicity against rkn meloidogyne incognita, that makes this strain to be a good bionematicide candidate for controlling of rkns. here, we firstly report the complete genome of a. faecalis zd02 and describe its feat ... | 2016 | 26656226 |
| metagenomic insights into communities, functions of endophytes, and their associates with infection by root-knot nematode, meloidogyne incognita, in tomato roots. | endophytes are known to play important roles in plant's health and productivity. in this study, we investigated the root microbiome of tomato in association with infection by root knot nematodes. our objectives were to observe the effects and response of the bacterial endophytes before nematode attacks and to reveal the functional attributes of microbes in plant health and nematode pathogenesis. community analysis of root-associated microbiomes in healthy and nematode-infected tomatoes indicated ... | 2015 | 26603211 |
| gotu kola (centella asiatica): nutritional properties and plausible health benefits. | centella asiatica l. (gotu kola) is a nutritionally important plant and a valued traditional medicine in south east asia. in this review, the chemical composition, nutritional values, and health benefits of c. asiatica have been discussed in detail to emphasize its usage as traditional food and medicine. c. asiatica is one of the most commonly used green leafy vegetables (glvs) in some countries including sri lanka due to its high amounts of medicinally important triterpenoids and beneficial car ... | 2015 | 26602573 |
| inheritance and identification of a major quantitative trait locus (qtl) that confers resistance to meloidogyne incognita and a novel qtl for plant height in sweet sorghum. | southern root-knot nematodes (meloidogyne incognita) are a pest on many economically important row crop and vegetable species and management relies on chemicals, plant resistance, and cultural practices such as crop rotation. little is known about the inheritance of resistance to m. incognita or the genomic regions associated with resistance in sorghum (sorghum bicolor). in this study, an f2 population (n = 130) was developed between the resistant sweet sorghum cultivar 'honey drip' and the susc ... | 2015 | 26574655 |
| host transcriptional profiling at early and later stages of the compatible interaction between phaseolus vulgaris and meloidogyne incognita. | the root-knot nematode (meloidogyne incognita) is one of most devastating pathogens that attack the common bean crop. although there is evidence that some cultivars have race-specific resistance against m. incognita, these resistance sources have not proved effective, and nematodes are able to circumvent the host's defense system. we constructed rna-seq based libraries and used a high-throughput sequencing platform to analyze the plant responses to m. incognita. assessments were performed at 4 a ... | 2016 | 26551451 |
| exposure to double-stranded rna mediated by tobacco rattle virus leads to transcription up-regulation of effector gene mi-vap-2 from meloidogyne incognita and promotion of pathogenicity in progeny. | meloidogyne spp. are economically important plant parasites and cause enormous damage to agriculture world-wide. these nematodes use secreted effectors which modify host cells, allowing them to obtain the nutrients required for growth and development. a better understanding of the roles of effectors in nematode parasitism is critical for understanding the mechanisms of nematode-host interactions. in this study, mi-vap-2 of meloidogyne incognita, a gene encoding a venom allergen-like protein, was ... | 2016 | 26545953 |
| in vitro nematicidal activity of aryl hydrazones and comparative gc-ms metabolomics analysis. | a series of aryl hydrazones were synthesized and in vitro assayed for their activity on the root-knot nematode meloidogyne incognita. the phenylhydrazones of thiophene-2-carboxyaldehyde 5, 3-methyl-2-thiophenecarboxyaldehyde, 6, and salicylaldehyde, 2, were the most potent with ec50/48h values of 16.6 ± 2.2, 23.2 ± 2.7, and 24.3 ± 1.4 mg/l, respectively. a gc-ms metabolomics analysis, after in vitro nematode treatment with hydrazone 6 at 100 mg/l for 12 h, revealed elevated levels of fatty acids ... | 2015 | 26528945 |
| pasteuria penetrans for control of meloidogyne incognita on tomato and cucumber, and m. arenaria on snapdragon. | meloidogyne incognita and meloidogyne arenaria are important parasitic nematodes of vegetable and ornamental crops. microplot and greenhouse experiments were conducted to test commercial formulations of the biocontrol agent pasteuria penetrans for control of m. incognita on tomato and cucumber and m. arenaria on snapdragon. three methods of application for p. penetrans were assessed including seed, transplant, and post-plant treatments. efficacy in controlling galling and reproduction of the two ... | 2015 | 26527842 |
| effect of emamectin benzoate on root-knot nematodes and tomato yield. | southern root-knot nematode (meloidogyne incognita) is an obligate, sedentary endoparasite of more than 3000 plant species, that causes heavy economic losses and limit the development of protected agriculture of china. as a biological pesticide, emamectin benzoate has effectively prevented lepidopteran pests; however, its efficacy to control m. incognita remains unknown. the purpose of the present study was to test soil application of emamectin benzoate for management of m. incognita in laborato ... | 2015 | 26509680 |
| sequence composition of bac clones and ssr markers mapped to upland cotton chromosomes 11 and 21 targeting resistance to soil-borne pathogens. | genetic and physical framework mapping in cotton (gossypium spp.) were used to discover putative gene sequences involved in resistance to common soil-borne pathogens. chromosome (chr) 11 and its homoeologous chr 21 of upland cotton (g. hirsutum) are foci for discovery of resistance (r) or pathogen-induced r (pr) genes underlying qtls involved in response to root-knot nematode (meloidogyne incognita), reniform nematode (rotylenchulus reniformis), fusarium wilt (fusarium oxysporum f.sp. vasinfectu ... | 2015 | 26483808 |
| tracking changes in life-history traits related to unnecessary virulence in a plant-parasitic nematode. | evaluating trade-offs in life-history traits of plant pathogens is essential to understand the evolution and epidemiology of diseases. in particular, virulence costs when the corresponding host resistance gene is lacking play a major role in the adaptive biology of pathogens and contribute to the maintenance of their genetic diversity. here, we investigated whether life-history traits directly linked to the establishment of plant-nematode interactions, that is, ability to locate and move toward ... | 2015 | 26380696 |
| nematicidal triterpenoids from lantana camara. | a new triterpene, lancamarolide (1), and seven known triterpenes, oleanonic acid (2), lantadene a (3), 11α-hydroxy-3-oxours-12-en-28-oic acid (4), betulinic acid (5), lantadene b (6), and lantaninilic acid (7) were isolated from the aerial parts of lantana camara in the course of bioassay-guided isolation, and their nematicidal activities against meloidogyne incognita, the root knot nematode, were carried out. oleanonic acid was found to be the most active compound and exhibited 80% mortality af ... | 2015 | 26363887 |