Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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zearalenone uptake and biotransformation in micropropagated triticum durum desf. plants: a xenobolomic approach. | a model was set up to elucidate the uptake, translocation and metabolic fate of zearalenone (zen) in durum wheat. after treatment with zen, roots and shoots were profiled with lc-hrms. a comprehensive description of in planta zen biotransformation and a biotechnological evaluation of the model were obtained. up to 200 µg zen were removed by each plantlet after 14 days. most zen and its masked forms were retained in roots, while minimal amounts were detected in leaves. sixty-two chromatographic p ... | 2018 | 29368509 |
identification and validation of a new source of low grain cadmium accumulation in durum wheat. | cadmium (cd) is a heavy metal that has no known biological function and is toxic for many living organisms. the maximum level of cd concentration allowed in the international market for wheat grain is 0.2 mg kg-1. because phenotyping for cd uptake is expensive and time consuming, molecular markers associated with genes conferring low cd uptake would expedite selection and lead to the development of durum cultivars with reduced cd concentrations. here, we identified single nucleotide polymorphism ... | 2018 | 29352079 |
nanocarrier-mediated foliar zinc fertilization influences expression of metal homeostasis related genes in flag leaves and enhances gluten content in durum wheat. | wheat is the staple food for most of the world's population; however, it is a poor source of zinc. foliar fertilization of zinc via zinc loaded chitosan nanocarriers (zn-cnp) post-anthesis has proved to be a promising approach for grain zinc enhancement in durum wheat as evidenced in our earlier study. however, the molecular mechanism of uptake of zinc via zn-cnp remains unclear. | 2018 | 29342185 |
genome wide identification of wheat and brachypodium type one protein phosphatases and functional characterization of durum wheat tdpp1a. | reversible phosphorylation is an essential mechanism regulating signal transduction during development and environmental stress responses. an important number of dephosphorylation events in the cell are catalyzed by type one protein phosphatases (pp1), which catalytic activity is driven by the binding of regulatory proteins that control their substrate specificity or subcellular localization. plants harbor several pp1 isoforms accounting for large functional redundancies. while animal pp1s were ... | 2018 | 29338035 |
data set of enzyme fingerprinting of dietary fibre components (arabinoxylan and β-glucan) in old and modern italian durum wheat genotypes. | the data presented are related to the research article entitled "comparison of the dietary fibre composition of old and modern durum wheat (triticum turgidum spp. durum) genotypes" (de santis et al., 2018) [1]. this article provides details of the structures of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern italian durum wheat genotypes grown in two seasons, determined by enzyme digestion followed by high-performance anion-exchang ... | 2018 | 29326969 |
introgression of the aegilops speltoides su1-ph1 suppressor into wheat. | meiotic pairing between homoeologous chromosomes in polyploid wheat is inhibited by the ph1 locus on the long arm of chromosome 5 in the b genome. aegilops speltoides (genomes ss), the closest relative of the progenitor of the wheat b genome, is polymorphic for genetic suppression of ph1. using this polymorphism, two major suppressor loci, su1-ph1 and su2-ph1, have been mapped in ae. speltoides. su1-ph1 is located in the distal, high-recombination region of the long arm of the ae. speltoides chr ... | 2017 | 29326749 |
evolution of the crop rhizosphere: impact of domestication on root exudates in tetraploid wheat (triticum turgidum l.). | domestication has induced major genetic changes in crop plants to satisfy human needs and as a consequence of adaptation to agroecosystems. this adaptation might have affected root exudate composition, which can influence the interactions in the rhizosphere. here, using two different soil types (sand, soil), we provide an original example of the impact of domestication and crop evolution on root exudate composition through metabolite profiling of root exudates for a panel of 10 wheat genotypes t ... | 2017 | 29326736 |
contrasting root and photosynthesis traits in a large-acreage canadian durum variety and its distant parent of algerian origin for assembling drought/heat tolerance attributes. | in canada, the world's top exporter of high-protein durum, varietal development over its nearly six-decade history has been driven by a quest for yield improvement without compromise on grain protein content and other quality aspects. pelissier, a landrace selection from algeria that was introduced into north america more than a century ago and the variety strongfield that was released in 2004 are notable. pelissier, known to elaborate more roots and considered as drought tolerant, has been cult ... | 2017 | 29312927 |
micronised bran-enriched fresh egg tagliatelle: significance of gums addition on pasta technological features. | the aim of the work was to produce fibre-enriched fresh pasta based on micronised wheat bran and durum wheat semolina with appropriate techno-functional properties. wheat semolina was replaced with fine particle size (50% below 75 µm) wheat bran - up to 11.54% (w/w). a box-behnken design with randomised response surface methodology was used to determine a suitable combination of carboxymethylcellulose, xanthan gum and locust bean gum to improve pasta attributes: minimum cooking loss, maximum val ... | 2018 | 29307227 |
participation of green organs to grain filling in triticum turgidum var durum grown under mediterranean conditions. | in wheat, flag leaf, stem, chaff and awns contribute to grain filling through photosynthesis and/or re-mobilization. environmental and genetic effects on the relative contribution of each organ were examined by analyzing the consequences of sink-source manipulations (shading and excision) and by comparing carbon isotope discrimination (δ) values in dry matter (at maturity) and sap (two weeks after anthesis) in six durum wheat genotypes grown in two contrasting seasons. the contribution of flag l ... | 2017 | 29295600 |
molecular and cytogenetic characterization of the 5ds-5bs chromosome translocation conditioning soft kernel texture in durum wheat. | the durum wheat ( ssp. (desf.) husn.) cultivar soft svevo with a soft kernel texture was developed through a -mediated homoeologous 5ds-5bs chromosomal translocation. the soft kernel trait ( locus) derived from chromosome 5d of the common wheat ( l.) cultivar chinese spring. soft svevo was used as the donor parent to create near-isogenic soft durum germplasm. the size of the translocation, its estimated breakpoint, and the amount of chromosome 5bs translocated, if any, remain unknown. four near- ... | 2017 | 29293810 |
genome-wide association and prediction of grain and semolina quality traits in durum wheat breeding populations. | grain yield and semolina quality traits are essential selection criteria in durum wheat breeding. however, high phenotypic screening costs limit selection to relatively few breeding lines in late generations. this selection paradigm confers relatively low selection efficiency due to the advancement of undesirable lines into expensive yield trials for grain yield and quality trait testing. marker-aided selection can enhance selection efficiency, especially for traits that are difficult or costly ... | 2017 | 29293807 |
speed breeding is a powerful tool to accelerate crop research and breeding. | the growing human population and a changing environment have raised significant concern for global food security, with the current improvement rate of several important crops inadequate to meet future demand 1 . this slow improvement rate is attributed partly to the long generation times of crop plants. here, we present a method called 'speed breeding', which greatly shortens generation time and accelerates breeding and research programmes. speed breeding can be used to achieve up to 6 generatio ... | 2018 | 29292376 |
durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to tdhsp101c expression in early developmental stages. | the predicted world population increase along with climate changes threatens sustainable agricultural supply in the coming decades. it is therefore vital to understand crops diversity associated to abiotic stress response. heat stress is considered one of the major constrains on crops productivity thus it is essential to develop new approaches for a precocious and rigorous evaluation of varietal diversity regarding heat tolerance. plant cell membrane thermostability (cms) is a widely used method ... | 2017 | 29284056 |
effect of gaseous ozone treatments on don, microbial contaminants and technological parameters of wheat and semolina. | deoxynivalenol (don) is an important mycotoxin produced by several species of fusarium. it occurs often in wheat grain and is frequently associated with significant levels of its modified form don-3-glucoside (don-3-glc). ozone (o3) is a powerful disinfectant and oxidant, classified as gras (generally recognised as safe), that reacts easily with specific compounds including the mycotoxins aflatoxins, ochratoxin a, trichothecenes and zearalenone. it degrades don in aqueous solution and can be eff ... | 2018 | 29279049 |
lutein esterification in wheat flour increases the carotenoid retention and is induced by storage temperatures. | the present study aimed to evaluate the effects of long-term storage on the carotenoid pigments present in whole-grain flours prepared from durum wheat and tritordeum. as expected, higher storage temperatures showed a catabolic effect, which was very marked for free carotenoid pigments. surprisingly, for both cereal genotypes, the thermal conditions favoured the synthesis of lutein esters, leading to an enhanced stability, slower degradation, and, subsequently, a greater carotenoid retention. th ... | 2017 | 29232927 |
fusarium head blight incidence and mycotoxin accumulation in three durum wheat cultivars in relation to sowing date and density. | durum wheat (triticum turgidum var. durum) is an important crop in europe, particularly in the mediterranean countries. fusarium head blight (fhb) is considered as one of the most damaging diseases, resulting in yield and quality reduction as well as contamination of grain with mycotoxins. three winter durum wheat cultivars originating from austria, slovakia, and poland were analyzed during 2012-2014 seasons for fhb incidence and fusarium mycotoxin accumulation in harvested grain. moreover, the ... | 2017 | 29209889 |
genetic impact of rht dwarfing genes on grain micronutrients concentration in wheat. | wheat is a major staple food crop providing about 20% of dietary energy and proteins, and food products made of whole grain wheat are a major source of micronutrients like zinc (zn), iron (fe), manganese (mn), magnesium (mg), vitamin b and e. wheat provides about 40% intake of essential micronutrients by humans in the developing countries relying on wheat based diets. varieties with genetically enhanced levels of grain micronutrient concentrations can provide a cost-effective and sustainable opt ... | 2017 | 29200604 |
hydrothermal grain pre-processing and ultra-fine milling for the production of durum wheat flour fractions with high nutritional value. | this work describes a process for producing durum wheat flour fractions with high potential nutritional value using grain pre-milling hydrothermal treatment and ultra-fine grinding (micronization), coupled with air classification. the difference of bioactive value of the flour fractions in relation to dietary fibre and phenolic compounds was monitored on four durum wheat cultivars by analysing total arabinoxylans, water extractable arabinoxylans and 5- n-alkylresorcinols. the extractability of t ... | 2017 | 29186998 |
development and characterization of couscous-like product using bulgur flour as by-product. | couscous is produced traditionally by agglomeration of triticum durum semolina with water. the aims of this study were: to produce couscous-like product by substitution of semolina with bulgur by-product (undersize bulgur); to find optimum quantity of bulgur flour and processing conditions. in order to determine the optimum processing parameters and recipes; 0, 25 and 50% of bulgur containing couscous-like samples were prepared. the color, yield, sensory properties, total phenol and flavonoid co ... | 2017 | 29184252 |
causal agents of fusarium head blight of durum wheat (triticum durum desf.) in central italy and their in vitro biosynthesis of secondary metabolites. | durum wheat samples harvested in central italy (umbria) were analyzed to: evaluate the occurrence of the fungal community in the grains, molecularly identify the fusarium spp. which are part of the fusarium head blight (fhb) complex and characterize the in vitro secondary metabolite profiles of a subset of fusarium strains. the fusarium genus was one of the main components of the durum wheat fungal community. the fhb complex was composed of eight species: fusarium avenaceum (61%), f. graminearum ... | 2018 | 29173624 |
genetics of na+ exclusion and salinity tolerance in afghani durum wheat landraces. | selecting for low concentration of na+ in the shoot provides one approach for tackling salinity stress that adversely affects crop production. novel alleles for na+ exclusion can be identified and then introduced into elite crop cultivars. | 2017 | 29157217 |
plant organ cultures as masked mycotoxin biofactories: deciphering the fate of zearalenone in micropropagated durum wheat roots and leaves. | "masked mycotoxins" senso strictu are conjugates of mycotoxins resulting from metabolic pathways activated by the interplay between pathogenic fungi and infected plants. zearalenone, an estrogenic mycotoxin produced by fusarium spp, was the first masked mycotoxin ever described in the literature, but its biotransformation has been studied to a lesser extent if compared to other compounds such as deoxynivalenol. we presented herein the first application of organ and tissue culture techniques to s ... | 2017 | 29145415 |
a novel protein tyrosine phosphatase like phytase from lactobacillus fermentum nkn51: cloning, characterization and application in mineral release for food technology applications. | a novel protein tyrosine phosphatase like phytase (ptplp), designated as phylf from probiotic bacterium lactobacillus fermentum nkn51 was identified, cloned, expressed and characterized. the recombinant phylf showed specific activity of 174.5 u/mg. phylf exhibited strict specificity towards phytate and optimum temperature at 60 °c, ph 5.0 and ionic strength of 100 mm. km and kcat of phylf for phytate were 0.773 mm and 84.31 s-1, respectively. phylf exhibited high resistance against oxidative ina ... | 2017 | 29145111 |
evaluation of antioxidative and diabetes-preventive properties of an ancient grain, kamut® khorasan wheat, in healthy volunteers. | recently, there was an increasing interest on the use of ancient grains because of their better health-related composition. the aim of this study was to evaluate in healthy human subjects the antioxidative and diabetes-preventive properties of ancient kamut® khorasan wheat compared to modern wheat. | 2017 | 29143934 |
structural disorder and induced folding within two cereal, aba stress and ripening (asr) proteins. | abscisic acid (aba), stress and ripening (asr) proteins are plant-specific proteins involved in plant response to multiple abiotic stresses. we previously isolated the asr genes and cdnas from durum wheat (ttasr1) and barley (hvasr1). here, we show that hvasr1 and ttasr1 are consistently predicted to be disordered and further confirm this experimentally. addition of glycerol, which mimics dehydration, triggers a gain of structure in both proteins. limited proteolysis showed that they are highly ... | 2017 | 29138428 |
comparison of the dietary fibre composition of old and modern durum wheat (triticum turgidum spp. durum) genotypes. | it has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats. we therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern italian durum wheats. no differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina. the study t ... | 2018 | 29120786 |
traits explaining durum wheat (triticum turgidum l. spp. durum) yield in dry chilean mediterranean environments. | yield under water stress (ys) is used as the main criterion in the selection of wheat varieties for dry mediterranean environments. it has been proposed that selection of genotypes using ys assisted by morphological and physiological traits associated with ys is more efficient in selecting high yielding genotypes for dry environments. a study was carried out at the antumapu experiment station of the university of chile, located in santiago, chile (33° 40's and 70° 38' w). the objective was to ev ... | 2017 | 29104578 |
identification and characterization of sr13, a tetraploid wheat gene that confers resistance to the ug99 stem rust race group. | the puccinia graminis f. sp. tritici (pgt) ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. the durum wheat (triticum turgidum ssp. durum) gene sr13 confers resistance to ug99 and other virulent races, and is more effective at high temperatures. using map-based cloning, we delimited a candidate region including two linked genes encoding coiled-coil nucleotide-binding leucine-rich repeat proteins designated c ... | 2017 | 29078294 |
b genome specific polymorphism in the tddrf1 gene is in relationship with grain yield. | a and b genome copies of drf1 gene in durum wheat were isolated and sequenced using gene variability. b genome specific polymorphism resulted, in a ril population, in relationship with grain yield mainly in drought condition. drought tolerance is one of the main components of yield potential and stability, and its improvement is a major challenge to breeders. transcription factors are considered among the best candidate genes for developing functional markers, since they are components of the si ... | 2018 | 29075873 |
td4in2: a drought-responsive durum wheat (triticum durum desf.) gene coding for a resistance like protein with serine/threonine protein kinase, nucleotide binding site and leucine rich domains. | wheat, the main food source for a third of world population, appears strongly under threat because of predicted increasing temperatures coupled to drought. plant complex molecular response to drought stress relies on the gene network controlling cell reactions to abiotic stress. in the natural environment, plants are subjected to the combination of abiotic and biotic stresses. also the response of plants to biotic stress, to cope with pathogens, involves the activation of a molecular network. in ... | 2017 | 29065389 |
correction: single marker and haplotype-based association analysis of semolina and pasta colour in elite durum wheat breeding lines using a high-density consensus map. | [this corrects the article doi: 10.1371/journal.pone.0170941.]. | 2017 | 29065160 |
milling overrides cultivar, leavening agent and baking mode on chemical and rheological traits and sensory perception of durum wheat breads. | smell and aroma are important determinants of consumer acceptance, so gaining deeper insight into bread smell and aroma perception is a research goal. sixteen combinations of four variables were investigated, to evaluate the contributions of bread chemical and rheological properties and volatile organic compounds (vocs) towards sensory acceptability of breads: genotypes (landrace vs. modern); types of flour (wholemeal vs. semolina); leavening agents (brewing yeast vs. sourdough starter); and bak ... | 2017 | 29051605 |
mineral composition of durum wheat grain and pasta under increasing atmospheric co2 concentrations. | the concentrations of 10 minerals were investigated in the grain of 12 durum wheat genotypes grown under free air co2 enrichment conditions, and in four of their derived pasta samples, using inductively coupled plasma mass spectrometry. compared to ambient co2 (400ppm; amb), under elevated co2 (570ppm; ele), the micro-element and macro-element contents showed strong and significant decreases in the grain: mn, -28.3%; fe, -26.7%; zn, -21.9%; mg, -22.7%; mo, -40.4%; k, -22.4%; and ca, -19.5%. thes ... | 2018 | 29037725 |
total phenolic and yellow pigment contents and antioxidant activities of durum wheat milling fractions. | the aim of this study was to investigate the distribution of total yellow pigments, total phenolic compounds, and their antioxidant activities in various durum wheat milling fractions. carotenoid composition of yellow pigment extract was also examined using uplc. the abts radical scavenging activity of the milling fractions decreased in the order of short bran/bran > feed flour > flour/semolina in both total phenolic and total yellow pigment extracts. yellow pigments extracts from bran, short br ... | 2017 | 29036891 |
on the interactions among zinc availability and responses to ozone stress in durum wheat seedlings. | seedlings of durum wheat [triticum turgidum subsp. durum (desf.) husn] were exposed to zinc nutrition and to ozone (o3) in a factorial combination: adequate (+zn treatment) or no zn (-zn) in the nutrient solution, followed by exposure to either ozone-free air (filtered air, fa) or to 150 nl l-1 ozone (o3) for 4 h. although omitting zn from the nutrient solution failed to impose a genuine zn deficiency, -zn*fa durum wheat seedlings showed a typical deficiency behaviour, i.e. zn mobilisation from ... | 2017 | 28994034 |
variations in yield and gluten proteins in durum wheat varieties under late-season foliar versus soil application of nitrogen fertilizer in a northern mediterranean environment. | with the increasing demand for high-quality foodstuffs and concern for environmental sustainability, late-season nitrogen (n) foliar fertilization of common wheat is now an important and widespread practice. this study investigated the effects of late-season foliar versus soil n fertilization on yield and protein content of four varieties of durum wheat, aureo, ariosto, biensur and liberdur, in a three-year field trial in northern italy. | 2017 | 28992373 |
tritordeum: a novel cereal for food processing with good acceptability and significant reduction in gluten immunogenic peptides in comparison with wheat. | tritordeum is a novel cereal obtained from the hybridization between durum wheat and a wild barley. this study evaluates acceptance, digestibility and immunotoxic properties of tritordeum, a novel cereal for food processing. nineteen healthy volunteers participated in a study with different diets to compare tritordeum bread with wheat and gluten-free breads. | 2017 | 28963718 |
genome-wide association study of septoria tritici blotch resistance in ethiopian durum wheat landraces. | septoria tritici blotch (stb) is a devastating fungal disease affecting durum and bread wheat cultivation worldwide. the identification, development, and employment of resistant wheat genetic material is the key to overcoming costs and limitations of fungicide treatments. the search for resistance sources in untapped genetic material may speed up the deployment of stb genetic resistance in the field. ethiopian durum wheat landraces represent a valuable source of such diversity. in this study, 31 ... | 2017 | 28959268 |
physico-chemical properties and sensory profile of durum wheat dittaino pdo (protected designation of origin) bread and quality of re-milled semolina used for its production. | to help future quality checks, we characterized the physico-chemical and sensory properties of dittaino bread, a sourdough-based durum wheat bread recently awarded with protected designation of origin mark, along with the quality features of re-milled semolina used for its production. semolina was checked for falling number (533-644s), protein content (12.0-12.3g/100gd.m.), gluten content (9.7-10.5g/100gd.m.), yellow index (18.0-21.0), water absorption (59.3-62.3g/100g), farinograph dough stabil ... | 2018 | 28958525 |
a mouse model of anaphylaxis and atopic dermatitis to salt-soluble wheat protein extract. | wheat allergy and other immune-mediated disorders triggered by wheat proteins are growing at an alarming rate for reasons not well understood. a mouse model to study hypersensitivity responses to salt-soluble wheat protein (sswp) extract is currently unavailable. here we tested the hypothesis that sswp extract from wheat will induce sensitization as well as allergic disease in mice. | 2017 | 28950276 |
a novel approach based on untargeted lipidomics reveals differences in the lipid pattern among durum and common wheat. | in the present work the possibility of using an untargeted metabolomic strategy to discriminate between common and durum wheat lipidome for an authenticity purpose was explored. a first study was conducted by analyzing 52 samples from two durum and common wheat varieties. afterwards, an extended and independent sample set (173 samples and five varieties) was used as a confirmatory study to verify the stability and consistency of the models obtained. putatively identified markers were evaluated a ... | 2018 | 28946342 |
real-time pcr for the detection of precise transgene copy number in wheat. | despite the unceasing advances in genetic transformation techniques, the success of common delivery methods still lies on the behavior of the integrated transgenes in the host genome. stability and expression of the introduced genes are influenced by several factors such as chromosomal location, transgene copy number and interaction with the host genotype. such factors are traditionally characterized by southern blot analysis, which can be time-consuming, laborious, and often unable to detect th ... | 2017 | 28913805 |
lc-hrms for characterizing durum wheat pasta production variability and consumer overall liking. | semolina pasta represents one of the most important dishes in italian cuisine worldwide. italy is the leader in its production and, recently, the worldwide diffusion of its production has begun to grow tremendously. the perceived quality of a food product, such as pasta, is a key feature that allows a company to increase and maintain the competitive advantage of a specific brand. the overall flavor perception of the consumer, therefore, has become as important as other key quality factors such a ... | 2017 | 28899452 |
polyphenols in raw and cooked cereals/pseudocereals/legume pasta and couscous. | pasta and couscous are popular foods manufactured (in their traditional form) from durum wheat semolina. in recent years, the consumers' quest for novel, functional, gluten-free, wholegrain foods has prompted the industry to manufacture new pasta and couscous products in which durum wheat has been partially or totally replaced by other vegetable flours. besides dietary fibre, these raw materials might be an interesting source of phytochemicals. in this work, 16 commercial samples of pasta and fo ... | 2017 | 28892013 |
effect of co-segregating markers on high-density genetic maps and prediction of map expansion using machine learning algorithms. | advances in sequencing and genotyping methods have enable cost-effective production of high throughput single nucleotide polymorphism (snp) markers, making them the choice for linkage mapping. as a result, many laboratories have developed high-throughput snp assays and built high-density genetic maps. however, the number of markers may, by orders of magnitude, exceed the resolution of recombination for a given population size so that only a minority of markers can accurately be ordered. another ... | 2017 | 28878789 |
salt stress reveals differential physiological, biochemical and molecular responses in t. monococcum and t. durum wheat genotypes. | salt stress responses implicate a complex mechanism and differ from plant species to another. in this study, we analyzed the physiological, biochemical and molecular responses to salt stress of the diploid wheat (t. monococcum) and compared to the tetraploid wheat (t. durum). our results showed that the diploid wheat cultivar (cv. turkey) is relatively tolerant to different salt stress conditions than the tetraploid wheat cultivar (cv. om rabia3). this tolerance was manifested by significant ger ... | 2017 | 28878491 |
rna sequencing and co-expressed long non-coding rna in modern and wild wheats. | there is an urgent need for the improvement of drought-tolerant bread and durum wheat. the huge and complex genome of bread wheat (bbaadd genome) stands as a vital obstruction for understanding the molecular mechanism underlying drought tolerance. however, tetraploid wheat (triticum turgidum ssp., bbaa genome) is an ancestor of modern bread wheat and offers an important model for studying the drought response due to its less complex genome. additionally, several wild relatives of tetraploid whea ... | 2017 | 28878329 |
arbuscular mycorrhizal symbiosis mitigates the negative effects of salinity on durum wheat. | arbuscular mycorrhizal (am) symbiosis is generally considered to be effective in ameliorating the plant tolerance to salt stress. unfortunately, the comprehension of the mechanisms implicated in salinity stress alleviation by am symbiosis is far from being complete. thus, an experiment was performed by growing durum wheat (triticum durum desf.) plants under salt-stress conditions to evaluate the influence of am symbiosis on both the plant growth and the regulation of a number of genes related to ... | 2017 | 28877207 |
the betaine profile of cereal flours unveils new and uncommon betaines. | we report the lc-esi-ms/ms determination of betaines in commercial flours of cereals and pseudocereals most utilized in human nutrition. results showed that glycine betaine, trigonelline, proline betaine, nε-trimethyllysine were metabolites common to all examined flours, whereas an uncommon betaine, valine betaine, and glutamine betaine were present only in flours of barley, rye, oat, durum wheat, winter wheat, triticum dicoccum and triticum monococcum. valine betaine and glutamine betaine, the ... | 2018 | 28873565 |
allelic variation at high-molecular weight and low-molecular weight glutenin subunit genes in moroccan bread wheat and durum wheat cultivars. | glutenin is a major protein fraction contributing to the functional properties of gluten and dough. the glutenin constitutes 30-40% of the protein in wheat flour and about half of that in gluten. it is essential to identify correct glutenin alleles and to improve wheat quality by selecting alleles that exert favorable effects. moroccan wheat cultivars are unique in west asia and north africa region, since many of them possess resistance to hessian fly, a pest, which is becoming important in othe ... | 2017 | 28868214 |
discovery of a novel stem rust resistance allele in durum wheat that exhibits differential reactions to ug99 isolates. | wheat stem rust, caused by puccinia graminis f. sp. tritici erikss. & e. henn, can incur yield losses on susceptible cultivars of durum wheat, triticum turgidum ssp. durum (desf.) husnot. though several durum cultivars possess the stem rust resistance gene sr13, additional genes in durum wheat effective against emerging virulent races have not been described. durum line 8155-b1 confers resistance against the p. graminis f. sp. tritici race ttkst, the variant race of the ug99 race group with addi ... | 2017 | 28855282 |
effect of long-term storage on the free and esterified carotenoids in durum wheat (triticum turgidum conv. durum) and tritordeum (×tritordeum ascherson et graebner) grains. | the effect of long-term storage on the carotenoid composition in durum wheat and tritordeum grains was studied. total carotenoid (mainly lutein) content decreased according to a temperature dependent first-order degradative kinetic model. the carotenoid retention was similar in both genotypes at the lower temperatures (71-73% at -32°c, 70% at 6°c and 55-56% at 20°c), whereas at the higher temperatures the pigment retention was higher in tritordeum (42% at 37°c; 10% at 50°c) than durum wheat (23% ... | 2017 | 28847425 |
mapping qtl for root and shoot morphological traits in a durum wheat × t. dicoccum segregating population at seedling stage. | a segregating population of 136 recombinant inbred lines derived from a cross between the durum wheat cv. "simeto" and the t. dicoccum accession "molise colli" was grown in soil and evaluated for a number of shoot and root morphological traits. a total of 17 quantitative trait loci (qtl) were identified for shoot dry weight, number of culms, and plant height and for root dry weight, volume, length, surface area, and number of forks and tips, on chromosomes 1b, 2a, 3a, 4b, 5b, 6a, 6b, and 7b. lod ... | 2017 | 28845431 |
new insights into the impacts of elevated co2, nitrogen, and temperature levels on the regulation of c and n metabolism in durum wheat using network analysis. | the use of correlation networks and hierarchical cluster analysis provides a framework to organize and study the coordination of parameters such as genes, metabolites, proteins and physiological parameters. we have analyzed 142 traits from primary c and n metabolism, including biochemical and gene expression analyses, in a range of 32 different growth conditions (various [co2] levels, temperatures, n supplies, growth stages and experimental methods). to test the integration of primary metabolism ... | 2018 | 28827159 |
durum wheat mirnas in response to nitrogen starvation at the grain filling stage. | durum wheat highly depends on nitrogen for seed development and yield, and the obtainment of varieties with a better nitrogen use efficiency is crucial to reduce production costs and environmental pollution. in this study, sequencing of two small rna libraries obtained from tissues of ciccio and svevo cultivars grown under nitrogen starvation conditions produced 84 novel, and 161 conserved mirnas. of these, 7 novel and 13 known mirnas were newly identified in this work. quantitative pcr analysis ... | 2017 | 28813501 |
genetic and molecular characterization of leaf rust resistance in two durum wheat landraces. | leaf rust, caused by puccinia triticina, is a constraint to durum wheat (triticum turgidum subsp. durum) production, and landraces are reported to be an important source of resistance. two portuguese landraces (aus26582 and aus26579) showed resistance against durum-specific p. triticina races and were crossed with a susceptible landrace (bansi) to develop recombinant inbred line (ril) populations. monogenic segregation for leaf rust resistance was observed among both ril populations. the underly ... | 2017 | 28812937 |
prediction of cp and starch concentrations in ruminal in situ studies and ruminal degradation of cereal grains using nirs. | ruminal in situ incubations are widely used to assess the nutritional value of feedstuffs for ruminants. in in situ methods, feed samples are ruminally incubated in indigestible bags over a predefined timespan and the disappearance of nutrients from the bags is recorded. to describe the degradation of specific nutrients, information on the concentration of feed samples and undegraded feed after in situ incubation ('bag residues') is needed. for cereal and pea grains, cp and starch (st) analyses ... | 2017 | 28770698 |
genetic diversity within a global panel of durum wheat (triticum durum) landraces and modern germplasm reveals the history of alleles exchange. | durum wheat is the 10th most important crop in the world, and its use traces back to the origin of agriculture. unfortunately, in the last century only part of the genetic diversity available for this species has been captured in modern varieties through breeding. here, the population structure and genetic diversity shared among elites and landraces collected from 32 countries was investigated. a total of 370 entries were genotyped with axiom 35k array to identify 8,173 segregating single nucleo ... | 2017 | 28769970 |
genome wide association study to identify the genetic base of smallholder farmer preferences of durum wheat traits. | smallholder agriculture involves millions of farmers worldwide. a methodical utilization of their traditional knowledge in modern breeding efforts may help the production of locally adapted varieties better addressing their needs. in this study, a combination of participatory approaches, genomics, and quantitative genetics is used to trace the genetic basis of smallholder farmer preferences of durum wheat traits. two smallholder communities evaluated 400 ethiopian wheat varieties, mostly landrac ... | 2017 | 28769945 |
differences in gluten protein composition between old and modern durum wheat genotypes in relation to 20th century breeding in italy. | the impact of breeding on grain yields of wheat varieties released during the 20th century has been extensively studied, whereas less information is available on the changes in gluten quality associated with effects on the amount and composition of glutenins and gliadins. in order to explore the effects of breeding during the 20th century on gluten quality of durum wheat for processing and health we have compared a set of old and modern italian genotypes grown under mediterranean conditions. the ... | 2017 | 28769550 |
variability in iron, zinc and phytic acid content in a worldwide collection of commercial durum wheat cultivars and the effect of reduced irrigation on these traits. | diets very rich in cereals have been associated with micronutrient malnutrition, and the biofortification of them, has been proposed as one of the best approaches to alleviate the problem. durum wheat is one of the main sources of calories and protein in many developing countries. in this study, 46 durum varieties grown under full and reduced irrigation, were analyzed for micronutrients and phytate content to determine the potential bioavailability of the micronutrients. the variation was 25.7-4 ... | 2017 | 28764025 |
inheritance and bulked segregant analysis of leaf rust and stem rust resistance in durum wheat genotypes. | leaf rust, caused by puccinia triticina (pt) and stem rust caused by puccinia graminis f. sp. tritici (pgt) are important diseases of durum wheat. this study determined the inheritance and genomic locations of leaf rust resistance (lr) genes to pt-race bbbqj and stem rust resistance (sr genes to pgt-race ttksk in durum accessions. eight leaf rust resistant genotypes were used to develop bi-parental populations. accessions, pi 192051 and pi 534304 were also resistant to pgt-race ttksk. the result ... | 2017 | 28745102 |
the quality of functional whole-meal durum wheat spaghetti as affected by inulin polymerization degree. | the use of inulin in pasta improves the nutritional value decreasing the glycemic index in the blood after pasta ingestion but it compromises the sensory characteristics in terms of elasticity, firmness, bulkiness and adhesiveness. thus, in this work, the impact of substituting whole-meal durum wheat flour with inulin on cooking quality, sensory and textural properties, digested starch and chemical composition of spaghetti was investigated. two types of inulin at two different concentrations (2% ... | 2017 | 28732922 |
evaluation of genetic diversity and host resistance to stem rust in usda nsgc durum wheat accessions. | the usda-ars national small grains collection (nsgc) maintains germplasm representing global diversity of small grains and their wild relatives. to evaluate the utility of the nsgc durum wheat ( l. ssp. ) accessions, we assessed genetic diversity and linkage disequilibrium (ld) patterns in a durum core subset containing 429 lines with spring growth habit originating from 64 countries worldwide. genetic diversity estimated using wheat single-nucleotide polymorphism (snp) markers showed considerab ... | 2017 | 28724063 |
predictive statistical modelling of cadmium content in durum wheat grain based on soil parameters. | regulatory limits on cadmium (cd) content in food products are tending to become stricter, especially in cereals, which are a major contributor to dietary intake of cd by humans. this is of particular importance for durum wheat, which accumulates more cd than bread wheat. the contamination of durum wheat grain by cd depends not only on the genotype but also to a large extent on soil cd availability. assessing the phytoavailability of cd for durum wheat is thus crucial, and appropriate methods ar ... | 2017 | 28712083 |
assessment of vegetation indices derived by uav imagery for durum wheat phenotyping under a water limited and heat stressed mediterranean environment. | there is growing interest for using spectral vegetation indices (svi) derived by unmanned aerial vehicle (uav) imagery as a fast and cost-efficient tool for plant phenotyping. the development of such tools is of paramount importance to continue progress through plant breeding, especially in the mediterranean basin, where climate change is expected to further increase yield uncertainty. in the present study, normalized difference vegetation index (ndvi), simple ratio (sr) and green normalized dif ... | 2017 | 28694819 |
cadmium concentration in grains of durum wheat (triticum turgidum l. subsp. durum). | heavy metal excess in soil represents a critical problem for crop productivity. among these pollutants, cadmium (cd) is one of the most dangerous in terms of food-chain contamination. two durum wheat near-isogenic lines (nils) and 12 commercial varieties (cultivars arcangelo, aureo, aziziah, cappelli, cirillo, creso, iride, maestrale, parsifal, russello, strongfield, and svevo) of durum wheat were exposed to a nontoxic level of cd to evaluate its concentration in grains, roots, and shoots, as we ... | 2017 | 28686843 |
genomic restructuring in f1 hordeum chilense × durum wheat hybrids and corresponding hexaploid tritordeum lines revealed by dna fingerprinting analyses. | 2017 | 28674217 | |
understanding the effects of genotype, growing year, and breeding on tunisian durum wheat allergenicity. 1. the baker's asthma case. | baker's asthma is a serious airway disease triggered by wheat protein cm3 α-amylase/trypsin inhibitor. the purpose of the present study was to investigate the impact of genotype and crop year on allergen cm3 α-amylase/trypsin inhibitor associated with baker's asthma. a historical series of tunisian durum wheat (100 accessions), derived from three crop years, was used to compare the amount of cm3 from landraces to advanced cultivars. cm3 protein quantification was assessed after an enzymatic clea ... | 2017 | 28644028 |
development of a high-density snp-based linkage map and detection of qtl for β-glucans, protein content, grain yield per spike and heading time in durum wheat. | high-density genetic linkage maps of crop species are particularly useful in detecting qualitative and quantitative trait loci for important agronomic traits and in improving the power of classical approaches to identify candidate genes. the aim of this study was to develop a high-density genetic linkage map in a durum wheat recombinant inbred lines population (ril) derived from two elite wheat cultivars and to identify, characterize and correlate quantitative trait loci (qtl) for β-glucan, prot ... | 2017 | 28635630 |
from seed to cooked pasta: influence of traditional and non-conventional transformation processes on total antioxidant capacity and phenolic acid content. | the aim of this work was to compare the traditional with a non-conventional (i.e. kernel micronisation) durum wheat milling process by monitoring the content of bound, conjugated and free phenolic acids (pas) and the level of the total antioxidant capacity (tac) occurring in the durum wheat pasta production chain, from seed to cooked pasta. the traditional transformation processes negatively influenced tac and pa content (40% and 89% decrease from seed to cooked pasta, respectively), mainly duri ... | 2018 | 28635340 |
understanding the effects of genotype, growing year, and breeding on tunisian durum wheat allergenicity. 2. the celiac disease case. | the aim of this study was to compare immunogenic and toxic gluten peptides related to celiac disease (cd). 100 accessions of genotypes selected during the 20th century in tunisia were in vitro digested and then analyzed by uplc/esi-ms technique using an isotopically labeled internal standard. the first manova confirmed a high variability in the content of immunogenic and toxic peptides reflecting high genetic diversity in the germplasm released during the past century in tunisia, consistently wi ... | 2017 | 28635270 |
characterization of aldehyde oxidase (ao) genes involved in the accumulation of carotenoid pigments in wheat grain. | aldehyde oxidase (ao) enzyme (ec 1.2.3.1) catalyzes the final steps of carotenoid catabolism and it is a key enzyme in the abscisic acid (aba) biosynthesis. ao isoforms are located in the cytosolic compartment of tissues in many plants, where induce the oxidation of aldehydes into carboxylic acid, and in addition, catalyze the hydroxylation of some heterocycles. the goal of the present study was to characterize the ao genes involved in the accumulation of carotenoid pigments in wheat grain, an i ... | 2017 | 28596779 |
snp-based association analysis for seedling traits in durum wheat (triticum turgidum l. durum (desf.)). | in the present study, 150 accessions of worldwide originated durum wheat germplasm (triticum turgidum spp. durum) were observed for major seedling traits and their growth. the accessions were evaluated for major seedling traits under controlled conditions of hydroponics at the 13th, 20th, 27th and 34th day-after germination. biomass traits were measured at the 34th day-after germination. correlation analysis was conducted among the seedling traits and three field traits at maturity, plant height ... | 2017 | 28588384 |
glutathione s-transferase related detoxification processes are correlated with receptor-mediated vacuolar sorting mechanisms. | triticum durum glutathione s-transferase z1 is specifically responsive to glyphosate. its expression influences the receptor-mediated vacuolar sorting mechanisms involved in tolerance mechanisms. a zeta subfamily glutathione s-transferase gene from triticum durum (cv cappelli) (tdgstz1) was characterized as part of a complex detoxification mechanism. the effect of different abiotic stresses on tdgstz1 revealed that the gene is unexpectedly responsive to glyphosate (gly) herbicide despite it shou ... | 2017 | 28577236 |
optimization of durum wheat bread enriched with bran. | an attempt is made in this study to balance the nutritional and sensory quality of bread. in particular, the formulation of a functional durum wheat bread enriched with bran at high concentration has been developed. organogel concentration and bran particle size have been used as process variables for bread optimization. bran concentration was increased at value as high as 15%, thus increasing the nutritional content, even though the sensory quality decreased. bread was scored barely acceptable ... | 2017 | 28572958 |
improved responses to elevated co2 in durum wheat at a low nitrate supply associated with the upregulation of photosynthetic genes and the activation of nitrate assimilation. | elevated co2 often leads to photosynthetic acclimation, and n availability may alter this response. we investigated whether the coordination of shoot-root n assimilation by elevated co2 may help to optimize the whole-plant n allocation and maximize photosynthesis in hydroponically-grown durum wheat at two no3- supplies in interaction with plant development. transcriptional and biochemical analyses were performed on flag leaves and roots. at anthesis, the improved photosynthetic acclimation respo ... | 2017 | 28554469 |
novel sources of stripe rust resistance identified by genome-wide association mapping in ethiopian durum wheat (triticum turgidum ssp. durum). | stripe rust of wheat, caused by puccinia striiformis f. sp. tritici (pst), is a global concern for wheat production, and has been increasingly destructive in ethiopia, as well as in the united states and in many other countries. as ethiopia has a long history of stripe rust epidemics, its native wheat germplasm harbors potentially valuable resistance loci. moreover, the ethiopian germplasm has been historically underutilized in breeding of modern wheat worldwide and thus the resistance alleles f ... | 2017 | 28553306 |
high-throughput snp genotyping of modern and wild emmer wheat for yield and root morphology using a combined association and linkage analysis. | durum wheat (triticum turgidum var. durum desf.) is a major world crop that is grown primarily in areas of the world that experience periodic drought, and therefore, breeding climate-resilient durum wheat is a priority. high-throughput single nucleotide polymorphism (snp) genotyping techniques have greatly increased the power of linkage and association mapping analyses for bread wheat, but as yet there is no durum wheat-specific platform available. in this study, we evaluate the new 384ht wheat ... | 2017 | 28550605 |
dissecting the old mediterranean durum wheat genetic architecture for phenology, biomass and yield formation by association mapping and qtl meta-analysis. | association mapping was used to identify genome regions affecting yield formation, crop phenology and crop biomass in a collection of 172 durum wheat landraces representative of the genetic diversity of ancient local durum varieties from the mediterranean basin. the collection was genotyped with 1,149 dart markers and phenotyped in spanish northern and southern locations during three years. a total of 245 significant marker trait associations (mtas) (p<0.01) were detected. some of these associat ... | 2017 | 28542488 |
transcriptome of wheat inflorescence development from spikelet initiation to floral patterning. | early reproductive development in cereals is crucial for final grain number per spike, and hence the yield potential of the crop. to date, however, no systematic analyses of gene expression profiles during this important process have been conducted for common wheat (triticum aestivum). here, we studied the transcriptome profiles at four stages of early wheat reproductive development, from spikelet initiation to floral organ differentiation. k-means clustering and stage-specific transcript identi ... | 0 | 28515146 |
the dead, hardened floral bracts of dispersal units of wild wheat function as storage for active hydrolases and in enhancing seedling vigor. | it is commonly assumed that the dead, hardened floral bracts of the dispersal unit of grasses have been evolved to protect seeds from predation and / or assist in fruit/caryopsis dispersal. while these structures have important agronomical and economical implications, their adaptive value has not been fully explored. we investigated the hypothesis that the maternally derived hardened floral bracts have been evolved not just as a means for caryopsis protection and dispersal, but also as storage f ... | 2017 | 28493968 |
differential representation of albumins and globulins during grain development in durum wheat and its possible functional consequences. | durum wheat (triticum turgidum ssp. durum (desf.) husn.) is an economically important crop used for the production of semolina, which is the basis of pasta and other food products. its grains provide proteins and starch for human consumption. grain development is a key process in wheat physiology; it is highly affected by a number of enzymes that control the metabolic processes governing accumulation of starch and storage proteins and ultimately grain weight. most of these enzymes are present in ... | 2017 | 28478307 |
abiotic conditions leading to fum gene expression and fumonisin accumulation by fusarium proliferatum strains grown on a wheat-based substrate. | fusarium proliferatum produces fumonisins b not only on maize but also on diverse crops including wheat. using a wheat-based medium, the effects of abiotic factors, temperature and water activity (aw), on growth, fumonisin biosynthesis, and expression of fum genes were compared for three f. proliferatum strains isolated from durum wheat in argentina. although all isolates showed similar profiles of growth, the fumonisin production profiles were slightly different. regarding fum gene transcriptio ... | 2017 | 28463723 |
phenotypic and molecular variation in drought tolerance of jordanian durum wheat (triticum durum desf.) landraces. | drought is considered one of the major constraints of plant growth and productivity worldwide. plants respond to drought through different mechanisms including physiological, biochemical, and gene expression modulation. studying these mechanisms will provide better understanding of drought response mechanisms and will help breeders in developing new cultivars. in this study, growth, biochemical, and molecular responses of four wheat (triticum durum desf.) landraces to drought stress (300 mm mann ... | 2017 | 28461720 |
epistatic determinism of durum wheat resistance to the wheat spindle streak mosaic virus. | the resistance of durum wheat to the wheat spindle streak mosaic virus (wssmv) is controlled by two main qtls on chromosomes 7a and 7b, with a huge epistatic effect. wheat spindle streak mosaic virus (wssmv) is a major disease of durum wheat in europe and north america. breeding wssmv-resistant cultivars is currently the only way to control the virus since no treatment is available. this paper reports studies of the inheritance of wssmv resistance using two related durum wheat populations obtain ... | 2017 | 28451771 |
transcriptomic response of durum wheat to nitrogen starvation. | nitrogen (n) is a key macronutrient representing a limiting factor for plant growth and development and affects productivity in wheat. in this study, durum wheat response to n chronic starvation during grain filling was investigated through a transcriptomic approach in roots, leaves/stems, flag leaf and spikes of cv. svevo. nitrogen stress negatively influenced plant height, tillering, flag leaf area, spike and seed traits, and total n content. rna-seq data revealed 4,626 differentially expresse ... | 2017 | 28446759 |
a rapid magnetic solid phase extraction method followed by liquid chromatography-tandem mass spectrometry analysis for the determination of mycotoxins in cereals. | mycotoxins can contaminate various food commodities, including cereals. moreover, mycotoxins of different classes can co-contaminate food, increasing human health risk. several analytical methods have been published in the literature dealing with mycotoxins determination in cereals. nevertheless, in the present work, the aim was to propose an easy and effective system for the extraction of six of the main mycotoxins from corn meal and durum wheat flour, i.e., the main four aflatoxins, ochratoxin ... | 0 | 28430148 |
occurrence of deoxynivalenol and deoxynivalenol-3-glucoside in durum wheat from argentina. | the occurrence of deoxynivalenol, 3- and 15-deoxynivalenol and deoxynivalenol-3-glucoside in 84 durum wheat samples, from the argentinean main growing area, was investigated during 2012/13 and 2013/14 using lc-ms/ms. deoxynivalenol was found in all samples at concentrations varying between <loq (50μg/kg) and 9480μg/kg. deoxynivalenol-3-glucoside was detected in 94% of the samples at concentrations ranging from <loq (50μg/kg) to 850μg/kg. moreover, the acetylated derivatives were also detected bu ... | 2017 | 28407973 |
effect of phytoliths for mitigating water stress in durum wheat. | the role of silicon (si) in alleviating biotic and abiotic stresses in crops is well evidenced by empirical studies; however, the mechanisms by which it works are still poorly known. the aim of this study is to determine whether or not phytolith composition and distribution in wheat are affected by drought and, if so, why. durum wheat was grown using hydroponics in the presence of polyethylene glycol (peg)-6000 to perform a water-stress simulation. we developed an original method for in situ ana ... | 2017 | 28394079 |
pentaploid wheat hybrids: applications, characterisation, and challenges. | interspecific hybridisation between hexaploid and tetraploid wheat species leads to the development of f1 pentaploid hybrids with unique chromosomal constitutions. pentaploid hybrids derived from bread wheat (triticum aestivum l.) and durum wheat (triticum turgidum spp. durum desf.) crosses can improve the genetic background of either parent by transferring traits of interest. the genetic variability derived from bread and durum wheat and transferred into pentaploid hybrids has the potential to ... | 2017 | 28367153 |
proteomic insight into the mitigation of wheat root drought stress by arbuscular mycorrhizae. | arbuscular mycorrhizal fungi (amf) are plant growth promoters that ameliorate plant-water relations and the nutrient uptake of wheat. in this work, two cultivars of triticum spp., a bread and a durum wheat, grown under drought stress and inoculated or not by amf, are evaluated through a shotgun proteomic approach. the amf association had beneficial effects as compared to non-mycorrhizal roots, in both bread and durum wheat. the beneficial symbiosis was confirmed by measuring morphological and ph ... | 2017 | 28366879 |
zinc complexed chitosan/tpp nanoparticles: a promising micronutrient nanocarrier suited for foliar application. | cultivation of cereals in zinc deficient soils leads to declined nutritional quality of grain. zinc deficiency in humans is a consequence of consumption of micronutrient deficient cereals as staple food. to achieve an increase in zinc density in grain, we evaluated zinc complexed chitosan nanoparticles (zn-cnp) as a potential 'nanocarrier' suited for foliar fertilization. zn-cnp were synthesized using tri-polyphosphate as a cross-linker. spherical zn-cnp (diameter 250-300nm) were positively char ... | 0 | 28363565 |
long noncoding mirna gene represses wheat β-diketone waxes. | the cuticle of terrestrial plants functions as a protective barrier against many biotic and abiotic stresses. in wheat and other triticeae, β-diketone waxes are major components of the epicuticular layer leading to the bluish-white glaucous trait in reproductive-age plants. glaucousness in durum wheat is controlled by a metabolic gene cluster at the wax1 (w1) locus and a dominant suppressor inhibitor of wax1 (iw1) on chromosome 2b. the wheat d subgenome from progenitor aegilops tauschii contains ... | 2017 | 28351975 |
identification of early represented gluten proteins during durum wheat grain development. | the time course of biosynthesis and accumulation of storage proteins in developing grains of durum wheat (triticum turgidum ssp. durum (desf.) husn.) pasta-quality reference cv. svevo was investigated at the protein level for the first time. seeds were harvested at key kernel developmental stages, namely, 3 (seed increase 3-fold in size), 5 (kernel development, water-ripe stage), 11 (kernel development, water-ripe stage), 16 (kernel full development, water-ripe stage), 21 (milk-ripe stage), and ... | 2017 | 28347138 |
quantitation of the immunodominant 33-mer peptide from α-gliadin in wheat flours by liquid chromatography tandem mass spectrometry. | coeliac disease (cd) is triggered by the ingestion of gluten proteins from wheat, rye, and barley. the 33-mer peptide from α2-gliadin has frequently been described as the most important cd-immunogenic sequence within gluten. however, from more than 890 published amino acid sequences of α-gliadins, only 19 sequences contain the 33-mer. in order to make a precise assessment of the importance of the 33-mer, it is necessary to elucidate which wheat species and cultivars contain the peptide and at wh ... | 2017 | 28327674 |
fcrav2, a gene with rogdi domain involved in fusarium head blight and crown rot on durum wheat caused by fusarium culmorum. | fusarium culmorum is a soil-borne fungal pathogen able to cause foot and root rot and fusarium head blight on small grain cereals, particularly on wheat and barley. it causes significant yield and quality loss and results in the contamination of kernels with type b trichothecene mycotoxins. knowledge on pathogenicity factors of this fungus is still limited. a transposon tagging approach based on the mimp1/impala double component system has allowed us to select a mutant altered in multiple metabo ... | 2017 | 28322011 |
identification and characterization of durum wheat micrornas in leaf and root tissues. | micrornas are a class of post-transcriptional regulators of plant developmental and physiological processes and responses to environmental stresses. here, we present the study regarding the annotation and characterization of mir genes conducted in durum wheat. we characterized the mirnaome of leaf and root tissues at tillering stage under two environmental conditions: irrigated with 100% (control) and 55% of evapotranspiration (early water stress). in total, 90 micrornas were identified, of whic ... | 2017 | 28321518 |
physical mapping of dna markers linked to stem rust resistance gene sr47 in durum wheat. | markers linked to stem rust resistance gene sr47 were physically mapped in three small aegilops speltoides chromosomal bins. five markers, including two pcr-based snp markers, were validated for marker-assisted selection. in durum wheat (triticum turgidum subsp. durum), the gene sr47 derived from aegilops speltoides conditions resistance to race ttksk (ug99) of the stem rust pathogen (puccinia graminis f. sp. tritici). sr47 is carried on small interstitial translocation chromosomes (ti2bl-2sl-2b ... | 2017 | 28286900 |