Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| survey on mycoplasma hyopneumoniae gilt acclimation practices in europe. | gilts are considered to play a key role in mycoplasma hyopneumoniae (m. hyopneumoniae) transmission and control. an effective gilt acclimation program should ideally reduce m. hyopneumoniae shedding at first farrowing, decreasing pre-weaning colonization prevalence and potential respiratory problems in fatteners. however, information on gilt acclimation practices is scarce in europe. the aim of this study was to identify current acclimation strategies for m. hyopneumoniae in europe using a quest ... | 2017 | 28852569 |
| etiology of acute respiratory disease in fattening pigs in finland. | the objective of our study was to clinically and etiologically investigate acute outbreaks of respiratory disease in finland. our study also aimed to evaluate the clinical use of various methods in diagnosing respiratory infections under field conditions and to describe the antimicrobial resistance profile of the main bacterial pathogen(s) found during the study. | 2017 | 28852568 |
| efficacy of one dose vaccination against experimental infection with two mycoplasma hyopneumoniae strains. | mycoplasma hyopneumoniae (m. hyopneumoniae) is the primary agent of enzootic pneumonia in pigs. pigs are often infected with different m. hyopneumoniae strains. this study assessed the efficacy of vaccination against experimental infection with two genetically different m. hyopneumoniae strains in weaned piglets. at 33 days of age (d0), 45 m. hyopneumoniae-free piglets were randomly assigned to three different groups: 1) negative control group (ncg; n = 5): not vaccinated, not infected, 2) posit ... | 2017 | 28851359 |
| update on mycoplasma hyopneumoniae infections in pigs: knowledge gaps for improved disease control. | mycoplasma hyopneumoniae (m. hyopneumoniae) is the primary pathogen of enzootic pneumonia, a chronic respiratory disease in pigs. infections occur worldwide and cause major economic losses to the pig industry. the present paper reviews the current knowledge on m. hyopneumoniae infections, with emphasis on identification and analysis of knowledge gaps for optimizing control of the disease. close contact between infected and susceptible pigs is the main route of m. hyopneumoniae transmission. mana ... | 2017 | 28834294 |
| mycoplasma hyopneumoniae detection in nylon-flocked and rayon-bud swabs. | the objective of this study was to compare the absorption and m. hyopneumoniae detection by real-time pcr using nylon-flocked and rayon-bud swabs. results of this study showed that absorption and detection of m. hyopneumoniae were significantly higher in nylon-flocked swabs with ct differences only ranging from 0.5 to 1.7. | 2017 | 28807761 |
| determinants for swine mycoplasmal pneumonia reproduction under experimental conditions: a systematic review and recursive partitioning analysis. | one of the main mycoplasma hyopneumoniae (m. hyopneumoniae) swine experimental model objectives is to reproduce mycoplasmal pneumonia (mp). unfortunately, experimental validated protocols to maximize the chance to successfully achieve lung lesions induced by m. hyopneumoniae are not available at the moment. thus, the objective of this work was to identify those factors that might have a major influence on the effective development of mp, measured as macroscopic lung lesions, under experimental c ... | 2017 | 28742802 |
| detection and phylogenetic analysis of mycoplasma hyopneumoniae from tibetan pigs in western china. | enzootic pneumonia (ep), often caused by mycoplasma hyopneumoniae, occurs in tibetan pigs between october and december in western china. the aim of this study was to determine the prevalence of m. hyopneumoniae in tibetan pig herds and also the prevalence of infection. m. hyopneumoniae was detected using enzyme-linked immunosorbent assay, polymerase chain reaction (pcr), and 16s rrna sequencing. twenty-nine inflammatory gross-lesions were observed in 155 lungs of slaughtered pigs. invasion of fo ... | 2017 | 28741040 |
| microbiome overview in swine lungs. | mycoplasma hyopneumoniae is the etiologic agent of swine enzootic pneumonia. however other mycoplasma species and secondary bacteria are found as inhabitants of the swine respiratory tract, which can be also related to disease. in the present study we have performed a total dna metagenomic analysis from the lungs of pigs kept in a field condition, with suggestive signals of enzootic pneumonia and without any infection signals to evaluate the bacteria variability of the lungs microbiota. librarie ... | 2017 | 28719637 |
| when is the best time to vaccinate piglets against mycoplasma hyopneumoniae. | 2017 | 28667141 | |
| detection of mycoplasma hyopneumoniae in naturally infected gilts over time. | mycoplasma hyopneumoniae causes a chronic respiratory infection in pigs and its transmission occurs mainly by direct contact and by vertical transmission (sow-to-piglet). the objective of this study was to assess the detection dynamics and persistence of m. hyopneumoniae natural infection in replacement gilts. forty-four twenty-day-old gilts were selected from a m. hyopneumoniae positive farm and followed up to one day prior to their first weaning. laryngeal swabs were collected every 30days, st ... | 2017 | 28619147 |
| comparison of sample types and diagnostic methods for in vivo detection of mycoplasma hyopneumoniae during early stages of infection. | detection of mycoplasma hyopneumoniae in live pigs during the early stages of infection is critical for timely implementation of control measures, but is technically challenging. this study compared the sensitivity of various sample types and diagnostic methods for detection of m. hyopneumoniae during the first 28days after experimental exposure. twenty-one 8-week old pigs were intra-tracheally inoculated on day 0 with m. hyopneumoniae strain 232. two age matched pigs were mock inoculated and ma ... | 2017 | 28619131 |
| occurrence of mycoplasma hyorhinis infections in fattening pigs and association with clinical signs and pathological lesions of enzootic pneumonia. | respiratory disorders in fattening pigs are of major concern worldwide. particularly enzootic pneumonia remains a problem for the pig industry. this chronic respiratory disease is primarily caused by mycoplasma hyopneumoniae (m. hyopneumoniae). however, more recently it was hypothesised that m. hyorhinis can also cause similar lung lesions. to investigate the relevance of m. hyorhinis as a cause of pneumonia in fattening pigs 10 farms in switzerland (considered free of enzootic pneumonia) and 20 ... | 2017 | 28619130 |
| mycoplasma hyopneumoniae vaccination at or shortly before weaning under field conditions: a randomised efficacy trial. | this study assessed the efficacy of two different mycoplasma hyopneumoniae vaccination programmes in relation to the time of weaning. eight hundred and twenty-eight piglets were randomly divided into three groups: group v1 was vaccinated three days before weaning, group v2 at weaning (21 days of age) and group nv was left non-vaccinated. vaccinations were performed using ingelvac mycoflex. after the nursery period, 306 pigs were allocated to fattening unit (f1) and 501 pigs to a second unit (f2) ... | 2017 | 28601840 |
| metabolic modeling of energy balances in mycoplasma hyopneumoniae shows that pyruvate addition increases growth rate. | mycoplasma hyopneumoniae is cultured on large-scale to produce antigen for inactivated whole-cell vaccines against respiratory disease in pigs. however, the fastidious nutrient requirements of this minimal bacterium and the low growth rate make it challenging to reach sufficient biomass yield for antigen production. in this study, we sequenced the genome of m. hyopneumoniae strain 11 and constructed a high quality constraint-based genome-scale metabolic model of 284 chemical reactions and 298 me ... | 2017 | 28600895 |
| comparison of gene expression of toll-like receptors and cytokines between piau and commercial line (landrace×large white crossbred) pigs vaccinated against pasteurella multocida type d. | we aimed to compare toll-like receptors (tlr) and cytokines expression in local piau breed and a commercial line (landrace×large white crossbred) pigs in response to vaccination against pasteurella multocida type d. seronegative gilts for pasteurella multocida type d and mycoplasma hyopneumoniae were used, from which peripheral blood mononuclear cells (pbmc) were collected in four time points (t0, t1, t2 and t3; before and after each vaccination dose). for bronchoalveolar lavage fluid cells (bal ... | 2017 | 28554143 |
| antimicrobial susceptibility monitoring of mycoplasma hyopneumoniae and mycoplasma bovis isolated in europe. | mycoplasma hyopneumoniae in pigs and mycoplasma bovis in cattle are major pathogens affecting livestock across europe and are the focus of the mycopath pan-european antimicrobial susceptibility monitoring programme. fifty m. hyopneumoniae isolates from belgium, spain and the united kingdom (uk), and 156 m. bovis isolates from france, hungary, spain and the uk that met specific criteria were tested for antimicrobial susceptibility in a central laboratory by using a microbroth dilution method. spe ... | 2017 | 28532800 |
| the use of oral fluids to monitor key pathogens in porcine respiratory disease complex. | the usefulness of oral fluid (of) sampling for surveillance of infections in pig populations is already accepted but its value as a tool to support investigations of porcine respiratory disease complex (prdc) has been less well studied. this study set out to describe detection patterns of porcine reproductive and respiratory syndrome virus (prrsv), porcine circovirus type 2 (pcv2), swine influenza virus type a (siv) and mycoplasma hyopneumoniae (m. hyo) among farms showing differing severity of ... | 2017 | 28405463 |
| provision of straw by a foraging tower -effect on tail biting in weaners and fattening pigs. | straw is one of the most effective rooting materials to reduce tail biting in pigs. a so-called foraging-tower (ft) provides only small quantities of straw compatible with liquid manure systems. the focus of the present study was on the effect of providing straw by ft in order to prevent tail biting in tail docked pigs. four consecutive batches of 160 pigs, randomly divided into a straw (sg) and a control group (cg) were followed up from weaning to slaughter. | 2017 | 28405460 |
| field efficacy of porcilis® pcv m hyo versus a licensed commercially available vaccine and placebo in the prevention of prdc in pigs on a french farm: a randomized controlled trial. | a controlled, randomised, and blinded trial performed on a conventional french farrow-to-finish farm compared the efficacy of a one-shot bivalent ready to use vaccine, porcilis® pcv m. hyo (group pcvm), to that of two commercial vaccines (ingelvac® circoflex® + ingelvac® mhyo, group icim), and to a placebo (group ctl), in preventing the health and economic impacts of porcine respiratory disease complex (prdc). | 2017 | 28405459 |
| comparison of the antimicrobial consumption in weaning pigs in danish sow herds with different vaccine purchase patterns during 2013. | there is growing concern about development of antimicrobial resistance due to use of antimicrobials (ams) in livestock production. identifying efficient alternatives, including vaccination, is a priority. the objective of this study was to compare the herd-level amount of ams prescribed for weaner pigs, between danish sow herds using varying combinations of vaccines against porcine circovirus type 2 (pcv2), mycoplasma hyopneumoniae (myc) and lawsonia intracellularis (law). it was hypothesised th ... | 2016 | 28405449 |
| modelling the within-herd transmission of mycoplasma hyopneumoniae in closed pig herds. | a discrete time, stochastic, compartmental model simulating the spread of mycoplasma hyopneumoniae within a batch of industrially raised pigs was developed to understand infection dynamics and to assess the impact of a range of husbandry practices. a 'disease severity' index was calculated based on the ratio between the cumulative numbers of acutely and chronically diseased and infectious pigs per day in each age category, divided by the length of time that pigs spent in this age category. this ... | 2016 | 28405436 |
| influence of spray dried porcine plasma in starter diets associated with a conventional vaccination program on wean to finish performance. | conventional vaccination programs using a single injection of a combined vaccine against porcine circovirus type 2 (pcv2) and mycoplasma hyopneumoniae (mhyo) can promote a strong immune response that reduces feed intake for 24 to 48 h post injection. often such vaccines are given around the time of weaning during a critical stress period in which feed intake is already compromised. spray dried porcine plasma (sdpp) is a protein source used in starter diets that increases post-weaning feed intake ... | 2016 | 28405430 |
| field efficacy study of a novel ready-to-use vaccine against mycoplasma hyopneumoniae and porcine circovirus type 2 in a greek farm. | the primary objective of this study was to assess the efficacy, under field conditions, of a novel ready-to use mycoplasma hyopneumoniae (m hyo) and porcine circovirus type 2 (pcv2) combination vaccine given to piglets as one vaccination (1-shot) at 3 weeks of age. the study was carried out according to a controlled, randomised, and blinded design in a greek pig herd with clinical m. hyo and subclinical pcv2 infection. moreover, based on serology at the time of vaccination, the average pcv2 titr ... | 2015 | 28405421 |
| a randomized controlled study on the efficacy of a novel combination vaccine against enzootic pneumonia (mycoplasma hyopneumoniae) and porcine circovirus type 2 (pcv2) in the presence of strong maternally derived pcv2 immunity in pigs. | mycoplasma hyopneumoniae (m. hyo) and porcine circovirus type 2 (pcv2) are major pathogens that cause significant health problems in swine worldwide. maternal derived immunity (mdi) has been suggested as a significant immediate defence factor for newborn piglets and may interfere with piglet's vaccination-induced immunity. the study aimed to assess the efficacy of a novel combination vaccine (consisting of pcv2 subunits and inactivated m. hyo strain j), against pcv2 and m. hyo natural infection ... | 2017 | 28388953 |
| potential use of local and systemic humoral immune response parameters to forecast mycoplasma hyopneumoniae associated lung lesions. | immunopathological events are key for the development of enzootic pneumonia (ep), which is macroscopically observed as cranioventral pulmonary consolidation (cvpc). this study aimed to investigate the putative association between the humoral immune response against mycoplasma hyopneumoniae (m. hyopneumoniae) and prevalence and extension of cvpc in 1) experimentally infected pigs, 2) slaughtered pigs and 3) sequentially necropsied pigs in a longitudinal study. cvpc was scored by means of the euro ... | 2017 | 28380065 |
| serum acute phase response induced by different vaccination protocols against circovirus type 2 and mycoplasma hyopneumoniae in piglets. | the purpose of this study was to compare the acute phase reaction (apr) induced by different vaccination protocols used against porcine circovirus (pcv) type-2 and mycoplasma hyopneumoniae (m.hyo), studying two acute phase proteins (apps) and changes in rectal temperature (rt). in addition, the possible influence of the time of vaccination and breed were analysed. in the first experiment, 40 commercial crossbred piglets were vaccinated, on the day of weaning, with flexcombo® (group a, n=20) or p ... | 2017 | 28324811 |
| pro-apoptotic effect of a mycoplasma hyopneumoniae putative type i signal peptidase on pk(15) swine cells. | mycoplasma hyopneumoniae is an economically significant swine pathogen that causes porcine enzootic pneumonia (pep). important processes for swine infection by m. hyopneumoniae depend on cell surface proteins, many of which are secreted by secretion pathways not completely elucidated so far. a putative type i signal peptidase (spase i), a possible component of a putative sec-dependent pathway, was annotated as a product of the sips gene in the pathogenic m. hyopneumoniae 7448 genome. this m. hyo ... | 2017 | 28284605 |
| a novel chimeric protein composed of recombinant mycoplasma hyopneumoniae antigens as a vaccine candidate evaluated in mice. | enzootic pneumonia (ep) is caused by the mycoplasma hyopneumoniae pathogenic bacteria, and it represents a significant respiratory disease that is responsible for major economic losses within the pig industry throughout the world. the bacterins that are currently commercially available have been proven to offer only partial protection against m. hyopneumoniae, and the development of more efficient vaccines is required. several recombinant antigens have been evaluated via different immunization s ... | 2017 | 28284602 |
| assessment of the adjuvant activity of mesoporous silica nanoparticles in recombinant mycoplasma hyopneumoniae antigen vaccines. | the adjuvant potential of two mesoporous silica nanoparticles (msns), sba-15 and sba-16, was assessed in combination with a recombinant hsp70 surface polypeptide domain from mycoplasma hyopneumoniae, the etiological agent of porcine enzootic pneumonia (pep). the recombinant antigen (hsp70212-600), previously shown as immunogenic in formulation with classic adjuvants, was used to immunize balb/c mice in combination with sba-15 or sba-16 msns, and the effects obtained with these formulations were ... | 2017 | 28194450 |
| no clear effect of initiating vaccination against common endemic infections on the amounts of prescribed antimicrobials for danish weaner and finishing pigs during 2007-2013. | it is often stated that vaccines may help reduce antimicrobial use in swine production. however, limited evidence is available outside clinical trials. we studied the change in amounts of antimicrobials prescribed for weaners and finishers in herds following initiation of vaccination against five common endemic infections: mycoplasma hyopneumoniae, actinobacillus pleuropneumoniae, porcine circovirus type ii, porcine reproductive and respiratory syndrome virus, and lawsonia intracellularis. compa ... | 2016 | 28138438 |
| impact of diversity of mycoplasma hyopneumoniae strains on lung lesions in slaughter pigs. | the importance of diversity of mycoplasma hyopneumoniae (m. hyopneumoniae) strains is not yet fully known. this study investigated the genetic diversity of m. hyopneumoniae strains in ten pig herds, and assessed associations between the presence of different strains of m. hyopneumoniae and lung lesions at slaughter. within each herd, three batches of slaughter pigs were investigated. at slaughter, from each batch, 20 post mortem bronchoalveolar lavage fluid samples were collected for multiple lo ... | 2017 | 28095890 |
| effects of mycoplasma hyopneumoniae on porcine nasal cavity dendritic cells. | mycoplasma hyopneumoniae (mhp) is the primary etiological agent responsible for swine enzootic pneumonia (ep), a disease that cause tremendous economic losses all over the swine industry. dendritic cells (dcs), the most effective antigen-presenting cells, are widely distributed beneath respiratory epithelium. dcs uptake and present antigens to t cells, to initiate protective immune responses or generate immune-mediated pathology in different infections. in this study, we investigated the changes ... | 2017 | 28061998 |
| repetitive elements in mycoplasma hyopneumoniae transcriptional regulation. | transcriptional regulation, a multiple-step process, is still poorly understood in the important pig pathogen mycoplasma hyopneumoniae. basic motifs like promoters and terminators have already been described, but no other cis-regulatory elements have been found. dna repeat sequences have been shown to be an interesting potential source of cis-regulatory elements. in this work, a genome-wide search for tandem and palindromic repetitive elements was performed in the intergenic regions of all codin ... | 2016 | 28005945 |
| secretomes of mycoplasma hyopneumoniae and mycoplasma flocculare reveal differences associated to pathogenesis. | mycoplasma hyopneumoniae and mycoplasma flocculare cohabit the porcine respiratory tract. however, m. hyopneumoniae causes the porcine enzootic pneumonia, while m. flocculare is a commensal bacterium. comparative analyses demonstrated high similarity between these species, which includes the sharing of all predicted virulence factors. nevertheless, studies related to soluble secretomes of mycoplasmas were little known, although they are important for bacterial-host interactions. the aim of this ... | 2017 | 28003119 |
| evolution and function of the mycoplasma hyopneumoniae peroxiredoxin, a 2-cys-like enzyme with a single cys residue. | the minimal genome of the mollicute mycoplasma hyopneumoniae, the etiological agent of porcine enzootic pneumonia, encodes a limited repertoire of antioxidant enzymes that include a single and atypical peroxiredoxin (mhprx), whose evolution and function were studied here. mhprx has only one catalytic cysteine, in contrast with some of its possible ancestors (2-cys peroxiredoxins), which have two. although it is more similar to 2-cys orthologs, mhprx can still function with a single peroxidatic c ... | 2017 | 27858147 |
| mycoplasma non-coding rna: identification of small rnas and targets. | bacterial non-coding rnas act by base-pairing as regulatory elements in crucial biological processes. we performed the identification of trans-encoded small rnas (srna) from the genomes of mycoplama hyopneumoniae, mycoplasma flocculare and mycoplasma hyorhinis, which are mycoplasma species that have been identified in the porcine respiratory system. | 2016 | 27801290 |
| selective medium for culture of mycoplasma hyopneumoniae. | the fastidious porcine respiratory pathogen mycoplasma hyopneumoniae has proven difficult to culture since it was first isolated in 1965. a reliable solid medium has been particularly challenging. moreover, clinical and pathological samples often contain the fast-growing m. hyorhinis which contaminates and overgrows m. hyopneumoniae in primary culture. the aim of this study was to optimise the culture medium for recovery of m. hyopneumoniae and to devise a medium for selection of m. hyopneumonia ... | 2016 | 27771062 |
| low temperature and dust favour in vitro survival of mycoplasma hyopneumoniae: time to revisit indirect transmission in pig housing. | porcine enzootic pneumonia (ep) caused by mycoplasma hyopneumoniae adversely affects pig welfare and is associated with major economic losses in the pig industry worldwide. transmission is predominantly by direct contact, but the role of indirect transmission remains poorly understood. this study examined survival of six m. hyopneumoniae isolates dried onto five different surfaces encountered in pig units and exposed to temperatures of 4, 25 and 37°c for up to 12 days. survival of the organisms ... | 2017 | 27759918 |
| serological patterns of actinobacillus pleuropneumoniae, mycoplasma hyopneumoniae, pasteurella multocida and streptococcus suis in pig herds affected by pleuritis. | respiratory illness is traditionally regarded as the disease of the growing pig, and has historically mainly been associated to bacterial infections with focus on mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae. these bacteria still are of great importance, but continuously increasing herd sizes have complicated the scenario and the influence of secondary invaders may have been increased. the aim of this study was to evaluate the presence of a. pleuropneumoniae and m. hyopneumoniae, ... | 2016 | 27716292 |
| bordetella bronchiseptica antigen enhances the production of mycoplasma hyopneumoniae antigen-specific immunoglobulin g in mice. | we previously demonstrated that bordetella bronchiseptica (b. bronchiseptica) antigen (ag) showed high immunostimulatory effects on mouse bone marrow cells (bms) while mycoplasma hyopneumoniae (m. hyopneumoniae) ag showed low. the focus of this study was if b. bronchiseptica ag may enhance m. hyopneumoniae ag-specific immune response, and also the host immune system can recognize both ags. mtt assay revealed that each or both ags did not significantly change the cell metabolic activity of bms. f ... | 2016 | 27659847 |
| in vitro antimicrobial activities of animal-used quinoxaline 1,4-di-n-oxides against mycobacteria, mycoplasma and fungi. | the quinoxaline 1,4-di-n-oxides (qdnos) were known as potent antibacterial agents. for the purpose of evaluating the bioactivity of existing animal-used qdnos drugs against representative pathogenic microorganism, the representative drugs of quinoxalines including cyadox, mequindox, quinocetone and their metabolites were submitted to the in vitro evaluation for antituberculosis, antimycoplasma, antifungal and antiviral activities. | 2016 | 27600955 |
| a survey of porcine respiratory disease complex (prdc) associated pathogens among commercial pig farms of korea via oral fluid method. | oral fluid analysis for monitoring herd is an interest in commercial pig farms in korea. the aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (prdc) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. the samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (rt-pcr) or standard pcr depending on microorganisms. the pathogens ... | 2016 | 27586468 |
| cross reactivity among the swine mycoplasmas as identified by protein microarray. | mycoplasmas are cell wall-less bacteria that infect a variety of animals in a species-specific manner. in swine, mycoplasma hyopneumoniae is the most virulent and presents the most disease and economic problems to the swine industry. serological assays are commonly used to assess colonization and disease, but antigenic cross-reactivity between m. hyopneumoniae and other mycoplasma species, most notably mycoplasma hyorhinis, mycoplasma hyosynoviae and mycoplasma flocculare, is a concern. the exte ... | 2016 | 27527784 |
| factors associated with herd-level prrsv infection and age-time to seroconversion in farrow-to-finish herds. | factors associated with porcine reproductive and respiratory syndrome virus (prrsv) infection were investigated in 109 herds. serums from four batches of pigs (4, 10, 16 and 22 weeks, 15 pigs/batch) were tested by elisa for prrsv antibodies. infection by mycoplasma hyopneumoniae (mhp), actinobacillus pleuropneumoniae, h1n1 and h1n2 swine influenza a viruses (swiav) and pcv2 were detected by specific serological or pcr tests. data related to herd characteristics, biosecurity, management housing a ... | 2016 | 27527759 |
| mycoplasma hyopneumoniae does not affect the interferon-related anti-viral response but predisposes the pig to a higher level of inflammation following swine influenza virus infection. | in pigs, influenza a viruses and mycoplasma hyopneumoniae (mhp) are major contributors to the porcine respiratory disease complex. pre-infection with mhp was previously shown experimentally to exacerbate the clinical outcomes of h1n1 infection during the first week after virus inoculation. in order to better understand the interactions between these pathogens, we aimed to assess very early responses (at 5, 24 and 48 h) after h1n1 infection in pigs pre-infected or not with mhp. clinical signs and ... | 2016 | 27498789 |
| mycoplasma hyorhinis is a potential pathogen of porcine respiratory disease complex that aggravates pneumonia caused by porcine reproductive and respiratory syndrome virus. | the porcine respiratory disease complex (prdc) caused by numerous bacterial and viral agents has a great impact on pig industry worldwide. although mycoplasma hyorhinis (mhr) has been frequently isolated from lung lesions from pigs with prdc, the pathological importance of mhr may have been underestimated. in this study, 383 serum samples obtained from seven herds with a history of prdc were tested for specific antibodies to mhr, mycoplasma hyopneumoniae (mhp), and porcine reproductive and respi ... | 2016 | 27436444 |
| retrospective analysis of bacterial and viral co-infections in pneumocystis spp. positive lung samples of austrian pigs with pneumonia. | aim of this study was the retrospective investigation of viral (porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), torque teno sus virus type 1 and 2 (ttsuv1, ttsuv2)) and bacterial (bordetella bronchiseptica (b. b.), mycoplasma hyopneumoniae (m. h.), and pasteurella multocida (p. m.)) co-infections in 110 pneumocystis spp. positive lung samples of austrian pigs with pneumonia. fifty-one % were positive for pcv2, 7% for prrsv, 22% for ttsuv1, 48% for t ... | 2016 | 27428002 |
| cyp1a1 mediates the suppression of major inflammatory cytokines in pulmonary alveolar macrophage (pam) cell lines caused by mycoplasma hyponeumoniae. | mycoplasmal pneumonia is a lung infection disease caused by mycoplasma hyopneumoniae in swine. we previously reported that cytochrome p450 1a1 (cyp1a1) expression was significantly downregulated in pigs infected with m. hyopneumoniae compared to the healthy controls. in this study, pulmonary alveolar macrophage (pam) cell lines with cyp1a1 overexpression or sirna-mediated cyp1a1 silencing were used to explore the biological function and regulatory mechanism of cyp1a1 gene expression changed on t ... | 2016 | 27377584 |
| genetic variability and limited clonality of mycoplasma hyorhinis in pig herds. | mycoplasma hyorhinis is a common inhabitant of the upper respiratory tract and tonsils of pigs. its role as a possible pathogen remains controversial. in order to gain more insight into the epidemiology and population structure of m. hyorhinis we genetically characterized 60 isolates by multi locus sequence typing (mlst). the m. hyorhinis strains originated from swiss and german pig herds with knowledge on the clinical background. the mlst scheme of tocqueville et al. (j. clin. microbiol. 2014) ... | 2016 | 27374901 |
| mycoplasma hyopneumoniae and mycoplasma flocculare differential domains from orthologous surface proteins induce distinct cellular immune responses in mice. | mycoplasma hyopneumoniae and mycoplasma flocculare are two genetically close species found in the swine respiratory tract. despite their similarities, while m. hyopneumoniae is the causative agent of porcine enzootic pneumonia, m. flocculare is a commensal bacterium. genomic and transcriptional comparative analyses so far failed to explain the difference in pathogenicity between these two species. we then hypothesized that such difference might be, at least in part, explained by amino acid seque ... | 2016 | 27283856 |
| porcine respiratory disease complex: interaction of vaccination and porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, and mycoplasma hyopneumoniae. | porcine respiratory disease is a multifactorial and complex disease caused by a combination of infectious pathogens, environmental stressors, differences in production systems, and various management practices; hence the name porcine respiratory disease complex (prdc) is used. porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), and mycoplasma hyopneumoniae are considered to be the most important pathogens that cause prdc. although interactions among the ... | 2016 | 27256017 |
| in vitro protective efficacy of lithium chloride against mycoplasma hyopneumoniae infection. | mycoplasma hyopneumoniae (m. hyopneumoniae) infection affects the swine industry. lithium chloride (licl), is a drug used to treat bipolar disorder and has also shown activity against bacterial and viral infections. herein, we evaluated the antibacterial activity of licl on pk-15 cells infected with m. hyopneumoniae. incubation of licl (40mm) with cells for 24h, did not significantly affect the cell viability. the qrt-pcr showed ~80% reduction in m. hyopneumoniae genome when licl added post-infe ... | 2016 | 27234543 |
| insights on the virulence of swine respiratory tract mycoplasmas through genome-scale metabolic modeling. | the respiratory tract of swine is colonized by several bacteria among which are three mycoplasma species: mycoplasma flocculare, mycoplasma hyopneumoniae and mycoplasma hyorhinis. while colonization by m. flocculare is virtually asymptomatic, m. hyopneumoniae is the causative agent of enzootic pneumonia and m. hyorhinis is present in cases of pneumonia, polyserositis and arthritis. the genomic resemblance among these three mycoplasma species combined with their different levels of pathogenicity ... | 2016 | 27178561 |
| a replicating plasmid-based vector for gfp expression in mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae (m. hyopneumoniae) causes porcine enzootic pneumonia (pep) that significantly affects the pig industry worldwide. despite the availability of the whole genome sequence, studies on the pathogenesis of this organism have been limited due to the lack of a genetic manipulation system. therefore, the aim of the current study was to generate a general gfp reporter vector based on a replicating plasmid. here, we describe the feasibility of gfp reporter expression in m. hyopneum ... | 2016 | 27173288 |
| induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes. | the purpose of this study was to assess the effect of three different inoculation routes into mycoplasmal pneumonia (mp) in pigs challenged with mycoplasma hyopneumoniae (m. hyopneumoniae). thirty six-week-old m. hyopneumoniae seronegative piglets were randomly assigned to four groups: three challenged groups with experimentally inoculated pigs by either the endotracheal (et; n = 8), intranasal (in; n = 8) or aerosol (ae; n = 8) routes and one uninfected group (control; n = 6). blood samples wer ... | 2016 | 27160189 |
| dynamics of mycoplasma hyopneumoniae seroconversion and infection in pigs in the three main production systems. | in this study, we investigated the dynamics of mycoplasma hyopneumoniae infections in 66 pig farms, with different production systems (one-, two-, and three-site systems), and considered different risk factors. serological assay was used to detect serum antibodies against m. hyopneumoniae and real time polymerase chain reaction (rt-pcr) was performed to detect m. hyopneumoniae dna in tracheobronchial swabs. results demonstrated that m. hyopneumoniae infection status was predominantly influenced ... | 2016 | 27142053 |
| comparison of 2 commercial single-dose mycoplasma hyopneumoniae vaccines and porcine reproductive and respiratory syndrome virus (prrsv) vaccines on pigs dually infected with m. hyopneumoniae and prrsv. | the objective of this study was to compare the efficacy of 2 different commercial mycoplasma hyopneumoniae vaccines and porcine reproductive and respiratory syndrome virus (prrsv) vaccines in regard to growth performance, microbiological and immunological analyses, and pathological observation from wean to finish (175 d of age). pigs were administered m. hyopneumoniae and prrsv vaccines at 7 and 21 d of age, respectively, or both at 21 d old and then challenged with both m. hyopneumoniae and prr ... | 2016 | 27127338 |
| development of mycoplasma hyopneumoniae recombinant vaccines. | mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (ep), a disease that affects swine production worldwide. vaccination is the most cost-effective strategy for the control and prevention of the disease. research using genome-based approach has the potential to elucidate the biology and pathogenesis of m. hyopneumoniae and contribute to the development of more effective vaccines. here, we describe the protocol for developing m. hyopneumoniae recombinant vaccines using r ... | 2016 | 27076288 |
| development of a blocking elisa for detection of mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein p65. | mycoplasma hyopneumoniae causes porcine enzootic pneumonia, an economically important disease of swine. a more sensitive and reliable method for detection of serum antibodies is needed for epidemiological investigations and to evaluate the effect of immunization. we expressed the m. hyopneumoniae protein p65 in escherichia coli and produced a monoclonal antibody (mab) that bound specifically to recombinant p65. using this mab, a blocking enzyme linked immunosorbent assay (elisa) was developed. t ... | 2016 | 27075114 |
| the effects of mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro pk-15 cells. | porcine circovirus type 2 (pcv2) is the causative agent of postweaning multisystemic wasting syndrome (pmws). mycoplasma hyopneumoniae (mhp) is a very well-known co-factor that potentially enhances pcv2 replication and thus the development of pmws. however, co-infection with mhp and pcv2 in vivo under different conditions can produce divergent clinical signs and lesions. in this study, pcv2 replication could be enhanced by subsequent co-inoculation with mhp (pcv2+mhp) in a time and dose dependen ... | 2016 | 27033909 |
| efficacy of mycoplasma hyopneumoniae vaccination before and at weaning against experimental challenge infection in pigs. | commercial bacterins are widely used at weaning to control mycoplasma hyopneumoniae infections in pigs. however, it is not known whether the efficacy of vaccinating against m. hyopneumoniae can be influenced by the weaning process when vaccination is applied at the day of weaning. the present study assessed the efficacy of a single m. hyopneumoniae vaccination (ingelvac mycoflex®) three days before weaning (v1) or at weaning (v2) against experimental challenge infection. four weeks after vaccina ... | 2016 | 27025924 |
| mycoplasma hyopneumoniae genetic variability within a swine operation. | the objective of our study was to characterize the mycoplasma hyopneumoniae genetic diversity within a swine operation comingling weaned pigs. bronchial swabs and tracheal aspirates were collected from 3 nursery-to-finish farms. during the finishing production stages, samples were obtained from mortalities and from live coughing pigs in rooms where mortality was not observed. a total of 105 samples were examined by a m. hyopneumoniae real-time polymerase chain reaction and subjected to genetic t ... | 2016 | 26965239 |
| relationship between expression of interleukin-5 and interleukin-13 by epithelial cells and bronchiolar changes in pigs infected with mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae (mh) is a bacterium that specifically infects the surface of bronchi and bronchioles of pigs without invading the host cells, and it is considered to be the primary agent of porcine enzootic pneumonia (pen). the present study investigates the morphological and immunohistological changes induced in bronchiolar epithelium by mh infection. lungs from 20 pigs with naturally occurring mh pneumonia were compared with those from 10 uninfected controls. bronchiolar epithelial he ... | 2017 | 26922858 |
| post-translational processing targets functionally diverse proteins in mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae is a genome-reduced, cell wall-less, bacterial pathogen with a predicted coding capacity of less than 700 proteins and is one of the smallest self-replicating pathogens. the cell surface of m. hyopneumoniae is extensively modified by processing events that target the p97 and p102 adhesin families. here, we present analyses of the proteome of m. hyopneumoniae-type strain j using protein-centric approaches (one- and two-dimensional gelc-ms/ms) that enabled us to focus on g ... | 2016 | 26865024 |
| a model to investigate the optimal seeder-to-naïve ratio for successful natural mycoplasma hyopneumoniae gilt exposure prior to entering the breeding herd. | due to the significance of mycoplasma hyopneumoniae as a swine respiratory pathogen, acclimation measures are taken into consideration when obtaining replacement gilts from negative sources to be introduced to endemically infected herds. the aim of this study was to evaluate the optimum seeder-to-naïve gilt ratio in a 4-week period for successful natural exposure to m. hyopneumoniae. sixty gilts were divided in two groups, 21 2-week old seeder gilts were inoculated with m. hyopneumoniae, and 39 ... | 2016 | 26854344 |
| increased fucosyl glycoconjugate by mycoplasma hyopneumoniae enhances adherences of pasteurella multocida type a in the ciliated epithelial cells of the respiratory tract. | the objective of this study was to elucidate the pathogenic mechanisms of how mycoplasma hyopneumoniae enhances secondary pasteurella multocida type a infection which leads to porcine enzootic pneumonia in infected pigs. sixteen pigs were experimentally infected with m. hyopneumoniae and then euthanized at 7, 14, 21 and 28 days post inoculation. in situ hybridization for m. hyopneumoniae dna and ulex europaeus agglutinin-i (uea-i) lectin histochemistry for fucosyl glycoconjugate, was performed i ... | 2016 | 26841756 |
| mycoplasma hyopneumoniae management in gilts. | 2016 | 26823313 | |
| assessment of mycoplasma hyopneumoniae-induced pneumonia using different lung lesion scoring systems: a comparative review. | mycoplasma hyopneumoniae is the primary aetiological agent of swine enzootic pneumonia (ep) and one of the major contributors to the porcine respiratory disease complex (prdc). gross lung lesions in pigs affected by ep consist of cranioventral pulmonary consolidation (cvpc), usually distributed bilaterally in the apical, intermediate, accessory and cranial parts of the diaphragmatic lobes. several lung scoring methods are currently in place for the evaluation of cvpc. the aims of this study were ... | 2017 | 26774274 |
| evaluating the risk of pathogen transmission from wild animals to domestic pigs in australia. | wild animals contribute to endemic infection in livestock as well as the introduction, reintroduction and maintenance of pathogens. the source of introduction of endemic diseases to a piggery is often unknown and the extent of wildlife contribution to such local spread is largely unexplored. the aim of the current study was to quantitatively assess the probability of domestic pigs being exposed to different pathogens from wild animals commonly found around commercial piggeries in australia. spec ... | 2016 | 26711303 |
| a new single-dose bivalent vaccine of porcine circovirus type 2 and mycoplasma hyopneumoniae elicits protective immunity and improves growth performance under field conditions. | the efficacy of the new single-dose bivalent vaccine of porcine circovirus type 2 (pcv2) and mycoplasma hyopneumoniae was evaluated under field conditions for registration as recommended by the republic of korea's animal, plant & fisheries quarantine & inspection agency. three farms were selected based on their history of co-infection with pcv2 and m. hyopneumoniae. on each farm, a total of 80 3-week-old pigs were randomly allocated to one of two treatment groups: (i) vaccinated (n=40) and (ii) ... | 2016 | 26711046 |
| molecular characterisation of mycoplasma hyorhinis isolated from pigs using pulsed-field gel electrophoresis and 16s rrna sequencing. | economic loss in pig breeding is common due to respiratory disorders, and mycoplasma hyopneumoniae and mycoplasma hyorhinis, namely, are the most common infectious agents. the aim of this study is to recover these mollicutes and detect their genotypic variations by pulsed-field gel electrophoresis (pfge) and sequencing the 16 s rrna gene. one hundred and twenty-six swabs from tonsil and nasal mucus of pigs with respiratory disorders were analysed. a total of 78 lungs were sampled, as well as two ... | 2015 | 26688737 |
| multiplex pcr and microarray for detection of swine respiratory pathogens. | porcine respiratory disease complex (prdc) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. no simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with prdc. furthermore, the detection of most of the bacterial pathogens implicated in prdc currently requires time-consuming culture-based methods that can take several days to obtain results. in this study, ... | 2015 | 26662640 |
| association between pneumocystis spp. and co-infections with bordetella bronchiseptica, mycoplasma hyopneumoniae and pasteurella multocida in austrian pigs with pneumonia. | in this retrospective study, 218 pig lung tissue samples were analyzed to examine a possible association between pneumocystis spp. using in situ hybridization, bordetella bronchiseptica (b.b.) using immunohistochemistry (ihc), mycoplasma hyopneumoniae (m.h.) by quantitative pcr, and pasteurella multocida (p.m.; ihc). compared to the bacterial agents (b.b., 5%; m.h., 30%; p.m., 23%), pneumocystis occurred with a higher prevalence (51%). co-infections with two or three pathogens were present in 28 ... | 2016 | 26654847 |
| efficacy of a new bivalent vaccine of porcine circovirus type 2 and mycoplasma hyopneumoniae (fostera™ pcv mh) under experimental conditions. | the objective of this study was to evaluate the efficacy of a new bivalent vaccine (fostera™ pcv mh, zoetis) of porcine circovirus type 2 (pcv2) and mycoplasma hyopneumoniae in growing pigs under experimental conditions. a total of 80 pigs were randomly divided into 8 groups (10 pigs per group). the pigs were administered the bivalent vaccine intramuscularly as a 2.0 ml dose at 21 days of age based on the manufacturer's instructions. three weeks after vaccination, the pigs were inoculated with e ... | 2016 | 26626212 |
| mlva typing of mycoplasma hyopneumoniae bacterins and field strains. | because of the lack of information about both the genetic characteristics of mycoplasma hyopneumoniae commercial vaccines and their relationship with field strains, the authors attempted to identify genetic subtypes of some m hyopneumoniae bacterins, and to compare them with m. hyopneumoniae field strains. six commercial m hyopneumoniae bacterins and 28 bronchoalveolar lavages from pigs at slaughter from three herds were analysed by multiple-locus variable number tandem repeat analysis (mlva) on ... | 2015 | 26495127 |
| apoptosis induced by lipid-associated membrane proteins from mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the mapk pathway. | lipid-associated membrane proteins (lamps) are important in the pathogenicity of the mycoplasma genus of bacteria. we investigated whether mycoplasma hyopneumoniae lamps have pathogenic potential by inducing apoptosis in a st. jude porcine lung epithelial cell line (sjpl). lamps from a pathogenic strain of m. hyopneumoniae (strain 232) were used in the research. our investigation made use of diamidino-phenylindole (dapi) and acridine orange/ethidium bromide (ao/eb) staining, terminal dexynucleot ... | 2015 | 26436384 |
| efficacy of combined vaccination against mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus in dually infected pigs. | porcine respiratory disease complex (prdc) is one of the main causes of economic losses for swine producers. this complex is due to a combination of different pathogens and their interactions. two major pathogens involved in prdc are mycoplasma hyopneumoniae (mhp) and porcine reproductive and respiratory syndrome virus (prrsv). the objectives of this study were (i) to develop an experimental model of dual mhp/prrsv infection in spf pigs with european strains of mhp and prrsv and (ii) to assess a ... | 2015 | 26422712 |
| a serosurvey for selected pathogens in greek european wild boar. | serum samples, collected from 94 european wild boar (sus scrofa) during the hunting seasons 2006 -2010 from different regions of greece, were examined in order to estimate the role of these wildlife species as reservoir of pathogens important for livestock and/or public health. | 2015 | 26392908 |
| use of tylvalosin in the control of porcine enzootic pneumonia. | the purpose of this study was to investigate the efficacy of tylvalosin (aivlosin water soluble granules, eco animal health) in drinking water for control of mycoplasma hyopneumoniae (m hyo) on a farm with chronic enzootic pneumonia (ep) problems and high prevalence of mycoplasma-like lesions at slaughter. | 2015 | 26392896 |
| increased mycoplasma hyopneumoniae disease prevalence in domestic hybrids among free-living wild boar. | wildlife immune genes are subject to natural selection exerted by pathogens. in contrast, domestic immune genes are largely protected from pathogen selection by veterinary care. introgression of domestic alleles into the wild could lead to increased disease susceptibility, but observations are scarce due to low introgression rates, low disease prevalence and reduced survival of domestic hybrids. here we report the first observation of a deleterious effect of domestic introgression on disease pre ... | 2015 | 26391376 |
| immunogenic properties and mycoplasmal pneumonia of swine (mps) lung lesions in large white pigs selected for higher peripheral blood immune capacity. | immunogenic properties and mycoplasmal pneumonia of swine (mps) lung lesions were compared between the immunity-selected large white line and the non-selected large white line. the selected large white line showed a higher level of pulmonary mps lesions compared with the non-selected large white line. subsequent to vaccination, the percentage of natural killer cells and t cells (cd3(+) cd4(+) cd8(-) and cd3(+) cd4(-) cd8(+) t cells) were significantly increased in the non-selected line but remai ... | 2016 | 26388420 |
| sonicated protein fractions of mycoplasma hyopneumoniae induce inflammatory responses and differential gene expression in a murine alveolar macrophage cell line. | mycoplasma hyopneumoniae is known to cause porcine enzootic pneumonia (ep), an important disease in swine production. the objective of this study was to examine the effects of sonicated protein fractions of m. hyopneumoniae on inflammatory response and gene expression in the murine alveolar macrophage mh-s cell line. the effects of sonicated protein fractions and intact m. hyopneumoniae on the gene expression of cytokines and inos were assessed using rt-pcr. the annealing control primer (acp)-ba ... | 2015 | 26370797 |
| swine influenza virus and association with the porcine respiratory disease complex in pig farms in southern brazil. | despite the putative endemic status of swine influenza a virus (swiav) infections, data on the occurrence of swine influenza outbreaks are scarce in brazil. the aim of this study was to detect and subtype swiavs from six outbreaks of porcine respiratory disease complex (prdc) in southern brazil. nasal swabs were collected from 66 piglets with signs of respiratory disease in six herds. lung tissue samples were collected from six necropsied animals. virus detection was performed by pcr screening a ... | 2016 | 26302164 |
| immunogenic properties of landrace pigs selected for resistance to mycoplasma pneumonia of swine. | mycoplasma pneumonia of swine (mps) lung lesions and immunogenic properties were compared between a landrace line that was genetically selected for reduced incidence of pulmonary mps lesions, and a non-selected landrace line. the mps-selected landrace line showed significantly lower degrees of pulmonary mps lesions compared with the non-selected landrace line. when changes in immunity before and after vaccination were compared, the percentage of b cells in the peripheral blood of the mps-selecte ... | 2016 | 26260893 |
| disease risks associated with free-ranging wild boar in saskatchewan. | this study investigated the disease status of saskatchewan's feral wild boar population. whole carcasses, tissue samples, and/or serum from 81 hunter-killed boars from saskatchewan were submitted to the canadian wildlife health cooperative (cwhc) between 2009 and 2014. serological tests were negative for prrs, h1n1, and h3n2 swine influenza, pcv-2, and tge/prcv in 22/22 boars and for toxoplasma gondii and mycoplasma hyopneumoniae in 20/20 boars. of 20 boars whose sera were tested 20 were positiv ... | 0 | 26246630 |
| genetic association of the porcine c9 complement component with hemolytic complement activity. | the complement system is a part of the natural immune regulation mechanism against invading pathogens. complement activation from three different pathways (classical, lectin, and alternative) leads to the formation of c5-convertase, an enzyme for cleavage of c5 into c5a and c5b, followed by c6, c7, c8, and c9 in membrane attack complex. the c9 is the last complement component of the terminal lytic pathway, which plays an important role in lysis of the target cells depending on its self-polymeriz ... | 2015 | 26194222 |
| assessment of litter prevalence of mycoplasma hyopneumoniae in preweaned piglets utilizing an antemortem tracheobronchial mucus collection technique and a real-time polymerase chain reaction assay. | the swine industry currently lacks validated antemortem methods of detecting baseline herd prevalence of mycoplasma hyopneumoniae. the focus of our study was to evaluate alternative antemortem detection techniques and to determine baseline litter prevalence in preweaned pig populations utilizing the selected technique and a real-time polymerase chain reaction (qpcr) assay. preliminary data was analyzed on weaned piglets with evidence of respiratory disease (n = 32). five sample types (antemortem ... | 2015 | 26179099 |
| impact of particulate matter and ammonia on average daily weight gain, mortality and lung lesions in pigs. | the present study investigated the simultaneous influence of particulate matter (pm10) and ammonia (nh3) on performance, lung lesions and the presence of mycoplasma hyopneumoniae (m. hyopneumoniae) in finishing pigs. a pig herd experiencing clinical problems of m. hyopneumoniae infections was selected. in total, 1095 finishing pigs of two replicates in eight compartments each were investigated during the entire finishing period (fp). indoor pm10 and nh3 were measured at regular intervals during ... | 2015 | 26148844 |
| mycoplasma hyopneumoniae infections in peri-weaned and post-weaned pigs in belgium and the netherlands: prevalence and associations with climatic conditions. | mycoplasma hyopneumoniae (m. hyo) is an important pathogen in modern intensive pig farming in europe. the objectives of the present study were (1) to use the tracheobronchial swab (tbs) technique to obtain data on the distribution of m. hyo infections in recently weaned pigs in belgium and the netherlands, and (2) to look for associations between infection prevalence and specific climatic conditions. one hundred and seventy-six pig herds were randomly selected and 30 piglets sampled on each farm ... | 2015 | 25981930 |
| a rapid and sensitive loop-mediated isothermal amplification procedure (lamp) for mycoplasma hyopneumoniae detection based on the p36 gene. | the aim of this study was to establish a method for sensitive and rapid diagnosis of mycoplasma hyopneumoniae in clinical specimens. to this effect, we employed three sets of primers specifically designed for amplification of nucleic acids under isothermal conditions. after optimization of reaction conditions, m. hyopneumoniae could be successfully detected at 63°c in 45 min through use of the loop-mediated isothermal amplification (lamp) assay. a positive reaction was identified visually as whi ... | 2015 | 25966242 |
| characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and mycoplasma hyopneumoniae vaccines. | vaccines consisting of subunit or inactivated bacteria/virus and potent adjuvants are widely used to control and prevent infectious diseases. because inactivated and subunit antigens are often less antigenic than live microbes, a growing need exists for the development of new and improved vaccine adjuvants that can elicit rapid and long-lasting immunity. here we describe the development and characterization of a novel oil-in-water emulsion, ow-14. ow-14 contains low-cost plant-based emulsifiers ... | 2015 | 25936722 |
| intrinsic terminators in mycoplasma hyopneumoniae transcription. | mycoplasma hyopneumoniae, an important pathogen of swine, exhibits a low guanine and cytosine (gc) content genome. m. hyopneumoniae genome is organised in long transcriptional units and promoter sequences have been mapped upstream of all transcription units. these analysis provided insights into the gene organisation and transcription initiation at the genome scale. however, the presence of transcriptional terminator sequences in the m. hyopneumoniae genome is poorly understood. | 2015 | 25886614 |
| exposure of extensively farmed wild boars (sus scrofa scrofa) to selected pig pathogens in greece. | increased density and distribution of wild boar populations are likely to promote interactions and transmission of certain pathogens, not only among wild boar but also from wild boar to livestock or humans and vice versa. | 2015 | 25774434 |
| complete genome sequence of mycoplasma flocculare strain ms42t (atcc 27399t). | mycoplasma flocculare is a commensal or low-virulence pathogen of swine. the complete 778,866-bp genome sequence of m. flocculare strain ms42(t) has been determined, enabling further comparison to genomes of the closely related pathogen mycoplasma hyopneumoniae. the absence of the p97 and glpd genes may contribute to the attenuated virulence of m. flocculare. | 2015 | 25767245 |
| investigating the role of free-ranging wild boar (sus scrofa) in the re-emergence of enzootic pneumonia in domestic pig herds: a pathological, prevalence and risk-factor study. | enzootic pneumonia (ep) caused by mycoplasma hyopneumoniae has a significant economic impact on domestic pig production. a control program carried out from 1999 to 2003 successfully reduced disease occurrence in domestic pigs in switzerland, but recurrent outbreaks suggested a potential role of free-ranging wild boar (sus scrofa) as a source of re-infection. since little is known on the epidemiology of ep in wild boar populations, our aims were: (1) to estimate the prevalence of m. hyopneumoniae ... | 2015 | 25747151 |
| exposure of feral swine (sus scrofa) in the united states to selected pathogens. | feral swine (sus scrofa) are widely distributed in the united states. in 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the us to evaluate exposure to important swine endemic pathogens. antibodies against porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) were found in 2.5% and 25.3% of tested sera, respectively. positive serological reactions against mycoplasma hyopneumoniae and actinobacillus p ... | 0 | 25673913 |
| comparison of immune responses to intranasal and intrapulmonary vaccinations with the attenuated mycoplasma hyopneumoniae 168 strain in pigs. | the aim of this study was to evaluate the immune responses to intranasal and intrapulmonary vaccinations with the attenuated mycoplasma hyopneumoniae (mhp) 168 strain in the local respiratory tract in pigs. twenty-four pigs were randomly divided into 4 groups: an intranasal immunization group, an intrapulmonary immunization group, an intramuscular immunization group and a control group. the levels of local respiratory tract cellular and humoral immune responses were investigated. the levels of i ... | 2015 | 25649413 |
| erysipelothrix rhusiopathiae and mycoplasma hyopneumoniae: the sensitivities of enzyme-linked immunosorbent assays for detecting vaccinated sows of unknown disease status using serum and colostrum, and the correlation of the results for sow serum, colostrum, and piglet serum. | due to relatively high concentrations of immunoglobulins, colostrum has the potential to improve the sensitivity of diagnostic tests for diseases in pigs when compared with serum. it is possible that colostrum could improve the sensitivity of the antibody enzyme-linked immunosorbent assay (elisa) compared with serum. colostrum is also essential for piglets, providing protection against infections in the first few weeks and months of life. the sensitivity of 2 commercially available elisas, one f ... | 2015 | 25613041 |
| mhj_0461 is a multifunctional leucine aminopeptidase on the surface of mycoplasma hyopneumoniae. | aminopeptidases are part of the arsenal of virulence factors produced by bacterial pathogens that inactivate host immune peptides. mycoplasma hyopneumoniae is a genome-reduced pathogen of swine that lacks the genetic repertoire to synthesize amino acids and relies on the host for availability of amino acids for growth. m. hyopneumoniae recruits plasmin(ogen) onto its cell surface via the p97 and p102 adhesins and the glutamyl aminopeptidase mhj_0125. plasmin plays an important role in regulating ... | 2015 | 25589579 |
| evaluation of adjuvant effects of fucoidan for improving vaccine efficacy. | fucoidan is a sulfated polysaccharide derived from brown seaweed, including fucus vesiculosus. this compound is known to have immunostimulatory effects on various types of immune cells including macrophages and dendritic cells. a recent study described the application of fucoidan as a vaccine adjuvant. vaccination is regarded as the most efficient prophylactic method for preventing harmful or epidemic diseases. to increase vaccine efficacy, effective adjuvants are needed. in the present study, w ... | 2015 | 25549218 |