Publications

culture-independent exploration of the teat apex microbiota of dairy cows reveals a wide bacterial species diversity.	due to their close proximity to the mammary gland tissue, the bacterial communities lining the teat apex of the udders from lactating cows influence udder health. denaturing gradient gel electrophoresis of the amplified v3 variable region of the 16s rrna gene was used as a culture-independent method to reveal the bacterial composition of 48 samples originating from the teat apices of twelve friesian-holstein dairy cows suffering from clinical mastitis in one quarter. the microbiota belonged to f ...	2011	22266158
regulation of the malic enzyme gene male by the transcriptional regulator malr in corynebacterium glutamicum.	corynebacterium glutamicum is a gram-positive nonpathogenic bacterium that is used for the biotechnological production of amino acids. here, we investigated the transcriptional control of the male gene encoding malic enzyme (male) in c. glutamicum atcc13032, which is known to involve the nitrogen regulator amtr. gel shift experiments using purified regulators rama and ramb revealed binding of these regulators to the male promoter. in dna-affinity purification experiments a hitherto uncharacteriz ...	2012	22261175
[effect of gamma-glutamyl kinase gene knock-out on metabolism in l-arginine-producing strain corynebacterium crenatum 8-193].	in order to optimize precursor supply for l-arginine biosynthesis, we constructed a corynebacterium crenatum 8-193 mutant with gamma-glutamyl kinase gene (prob) in-frame deletion. the effects of prob knock-out on physiological characteristics of the mutant were investigated.	2011	22260045
genome sequence of corynebacterium glutamicum atcc 14067, which provides insight into amino acid biosynthesis in coryneform bacteria.	we report the genome sequence of corynebacterium glutamicum atcc 14067 (once named brevibacterium flavum), which is useful for taxonomy research and further molecular breeding in amino acid production. preliminary comparison with those of the reported coryneform strains revealed some notable differences that might be related to the difficulties in molecular manipulation.	2012	22247536
genome sequence of corynebacterium casei ucma 3821, isolated from a smear-ripened cheese.	corynebacterium casei is one of the most prevalent species present on the surfaces of smear-ripened cheeses, where it contributes to the production of the desired organoleptic properties. here, we report the draft genome sequence of corynebacterium casei ucma 3821 to provide insights into its physiology.	2012	22247534
isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, bombyx mori, reared on different forage.	abstract the silkworm, bombyx mori l. (lepidoptera: bombycidae), an oligophagous insect that mainly feeds on mulberry leaves, is susceptible to entomopathogen infection when reared with tricuspid cudrania leaves. a total of 56 dominant bacterial strains, classified into 12 phylotypes based on bacteriological properties and analysis of 16s rrna genes, were isolated from the intestine of the fourth and fifth instar silkworm larvae. ten and seven phylotypes exist in the intestine of the silkworm ...	2011	22243438
corynebacterium diphtheriae 67-72p hemagglutinin, characterized as the protein dip0733, contributes to invasion and induction of apoptosis in hep-2 cells.	although corynebacterium diphtheriae has been classically described as an exclusively extracellular pathogen, there is growing evidence that it may be internalized by epithelial cells. the aim of the present report was to investigate the nature and involvement of the surface-exposed non-fimbrial 67-72 kda proteins (67-72p), previously characterized as adhesin/hemagglutinin, in c. diphtheriae internalization by hep-2 cells. transmission electron microscopy and bacterial internalization inhibition ...	2012	22239957
engineering corynebacterium glutamicum for the production of pyruvate.	a corynebacterium glutamicum strain with inactivated pyruvate dehydrogenase complex and a deletion of the gene encoding the pyruvate:quinone oxidoreductase produces about 19 mm l: -valine, 28 mm l: -alanine and about 55 mm pyruvate from 150 mm glucose. based on this double mutant c. glutamicum △acee △pqo, we engineered c. glutamicum for efficient production of pyruvate from glucose by additional deletion of the ldha gene encoding nad(+)-dependent l: -lactate dehydrogenase (ldha) and introduction ...	2012	22228312
Backbone and side chain (1)H, (13)C and (15)N resonance assignments of the catalytic domain of diphtheria toxin.	Diphtheria is a serious upper respiratory tract disease caused by the diphtheria toxin (DT) secreted from the bacteria Corynebacterium diphtheriae. Vaccination is the best way to protect against this infectious disease. Diphtheria vaccines are prepared by isolating, purifying and chemically deactivating DT. Although toxoids have been used for decades in immunization, there is still little understanding at the molecular level of the process of toxoid preparation, and how chemical treatment enhanc ...	2011	22205447
Enzymatic synthesis of S-adenosylhomocysteine: immobilization of recombinant S-adenosylhomocysteine hydrolase from Corynebacterium glutamicum (ATCC 13032).	Recombinant S-adenosylhomocysteine hydrolase from Corynebacterium glutamicum (CgSAHase) was covalently bound to Eupergit® C. The maximum yield of bound protein was 91% and the catalytic efficiency was 96.9%. When the kinetic results for the immobilized enzyme were compared with those for the soluble enzyme, no decrease in the catalytic efficiency of the former was detected. Both soluble and immobilized enzymes showed similar optimum pH and temperature ranges. The reuse of immobilized CgSAHase ca ...	2011	22202964
Solution NMR structures reveal a distinct architecture and provide first structures for protein domain family PF04536.	The protein family (Pfam) PF04536 is a broadly conserved domain family of unknown function (DUF477), with more than 1,350 members in prokaryotic and eukaryotic proteins. High-quality NMR structures of the N-terminal domain comprising residues 41-180 of the 684-residue protein CG2496 from Corynebacterium glutamicum and the N-terminal domain comprising residues 35-182 of the 435-residue protein PG0361 from Porphyromonas gingivalis both exhibit an a/ß fold comprised of a four-stranded ß-sheet, thre ...	2011	22198206
tuberculosis-like pneumonias by the aerobic actinomycetes rhodococcus, tsukamurella and gordonia.	the order actinomycetales includes phylogenetically diverse but morphologically similar aerobic and anaerobic organisms, exhibiting filamentous branching structures which fragment into rods or coccoid forms. lung pathogens of the order comprise mycobacterium, nocardia, corynebacterium, actinomyces, kytococcus, rothia, williamsia, as well as gordonia, tsukamurella and rhodococcus. particularly, members of the last three genera are uncommon aerobic agents of lung cavitations and tuberculosis(tb)-l ...	2011	22192786
mechanism of increased respiration in an h(+)-atpase-defective mutant of corynebacterium glutamicum.	we previously reported that a spontaneous h(+)-atpase-defective mutant of corynebacterium glutamicum, f172-8, derived from c. glutamicum atcc 14067, showed enhanced glucose consumption and respiration rates. to investigate the genome-based mechanism of enhanced respiration rate in such c. glutamicum mutants, a-1, an h(+)-atpase-defective mutant derived from c. glutamicum atcc 13032, which harbors the same point mutation as f172-8, was used in this study. a-1 showed similar fermentation profiles ...	2011	22188772
[blood culture results in specialistic unit for pulmonary illness and tuberculosis in bystra in 2008-2010].	the study presents the results of analysis of blood culture examination carried out in specialistyczny zespół chorób płuc i gruźlicy in bystra in 2008-2010. contamination incidence and number of blood samples taken from individual patients make it possible to assess if the procedure for taking material for microbiological examination is carried out. the analysis of positive test results allows for the evaluation of epidemiological condition of the hospital and it is also largely dependent on the ...	2011	22184946
[The use of MLVA for Corynebacterium diphtheriae genotyping--preliminary studies].	The complete genome sequence of strain NCTC 13129 C. diphtheriae were investigated in order to identify tandem repeats (VNTR). From 75 VNTR loci identified in the genome 14 were selected. Primers were designed and PCR conditions were optimized for amplification of the selected VNTR markers. Preliminary studies of usefulness of selected VNTR markers were conducted using a group of 28 C. diphtheriae strains. From 14 markers 8 were regarded as potentially useful. The diversity of individual markers ...	2011	22184915
[influence of substances produced by lipophilic corynebacterium cdc g1 zmf 3p13 on the microorganisms inhabiting human skin].	lipophilic species of corynebacterium inhabiting skin as residents produces substances that can regulate the composition of natural flora. research that was carried out concerned an influence of the substances produced by corynebacterium cdc g1 zmf 3p13 on the set of 22 bacterial strains (staphylococcus spp., corynebacterium spp., propionibacterium spp.) mutually existing on the skin and the set of 6 candida spp. isolated from patients. it was found out that the strain gives off into environment ...	2011	22184896
arabitol metabolism of corynebacterium glutamicum and its regulation by atlr.	expression profiling of corynebacterium glutamicum in comparison to a derivative deficient of the transcriptional regulator atlr (previously known as sugr or mtlr) revealed eight genes showing more than fourfold higher mrna levels in the mutant. four of these are located in direct vicinity of the atlr gene, i.e., xylb, rbtt, mtld, and sixa, annotated as encoding xylulokinase, ribitol transporter, mannitol 2-dehydrogenase, and phosphohistidine phosphatase, respectively. transcriptional analysis i ...	2011	22178972
Negative transcriptional control of biotin metabolism genes by the TetR-type regulator BioQ in biotin-auxotrophic Corynebacterium glutamicum ATCC 13032.	Genomic context analysis in actinobacteria revealed that biotin biosynthesis and transport (bio) genes are co-localized in several genomes with a gene encoding a transcription regulator of the TetR protein family, now named BioQ. Comparative analysis of the upstream regions of bio genes identified the common 13-bp palindromic motif TGAAC-N3-GTTAC as candidate BioQ-binding site. To verify the role of BioQ in controlling the transcription of bio genes, a deletion in the bioQ coding region (cg2309) ...	2011	22178235
Corynebacterium pyruviciproducens promotes the production of ovalbumin specific antibody via stimulating dendritic cell differentiation and up-regulating Th2 biased immune response.	Corynebacterium pyruviciproducens (C. pyruviciproducens), a newly discovered Corynebacterium, is gram-positive, non-flagellate, non-spore-forming lipophilic rod. No known pathogenic components of Corynebacteria have been found in this new bacterium, such as diphtheria toxin and tuberculostearic acid. In the present study, referring to Propionibacterium acnes (P. acnes), a well-known bacterial adjuvant, the stimulation of dendritic cells by C. pyruviciproducens was analyzed through detecting the ...	2011	22178104
genetic and biochemical characterization of corynebacterium glutamicum atp phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine.	atp phosphoribosyltransferase (atp-prt) catalyzes the condensation of atp and prpp at the first step of histidine biosynthesis and is regulated by a feedback inhibition from product histidine. here, we report the genetic and biochemical characterization of such an enzyme, hisg(cg), from corynebacterium glutamicum, including site-directed mutagenesis of the histidine-binding site for the first time. gene disruption and complementation experiments showed that hisg(cg) is essential for histidine bi ...	2011	22172596
erratum to: corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products.		2011	22159741
Biotin protein ligase from Corynebacterium glutamicum: role for growth and L: -lysine production.	Corynebacterium glutamicum is a biotin auxotrophic Gram-positive bacterium that is used for large-scale production of amino acids, especially of L: -glutamate and L: -lysine. It is known that biotin limitation triggers L: -glutamate production and that L: -lysine production can be increased by enhancing the activity of pyruvate carboxylase, one of two biotin-dependent proteins of C. glutamicum. The gene cg0814 (accession number YP_225000) has been annotated to code for putative biotin protein li ...	2011	22159614
Bacteremia following dental implant surgery: Preliminary results.	Objectives: The aims of this study were to investigate the incidence of bacteremia, bacteriology and antibiotic susceptibility against to causative bacteria associated with dental implant installation. Study Design: 30 generally healthy patients were enrolled in this study. Blood samples were collected at baseline and at 30 minutes after dental implant installation and 24 hours after dental implant surgery. Blood samples were cultured in a BACTEC system. The isolated bacteria were identified us ...	2012	22157668
Corynebacterium striatum bacteremia associated with central venous catheter infection.	Corynebacterium striatum (C striatum) has been considered a contaminant of blood culture in past decades. Here we report the case of a patient with acute deterioration of chronic renal failure. She received hemodialysis and died from C striatum bacteremia. By using a randomly amplified polymorphic DNA (RAPD) method, we found that an association existed between C striatum from the bloodstream and that from the central venous catheter. We suggest that C striatum could be a pathogen of bloodstream ...	2011	22154992
Purification, crystallization and preliminary X-ray diffraction studies of the arsenic repressor ArsR from Corynebacterium glutamicum.	ArsR is a member of the SmtB/ArsR family of metalloregulatory proteins that regulate prokaryotic arsenic-resistance operons. Here, the crystallization and preliminary X-ray diffraction studies of a cysteine-free derivative of ArsR from Corynebacterium glutamicum (CgArsR-C15/16/55S) are reported. CgArsR-C15/16/55S was expressed, purified, crystallized and X-ray diffraction data were collected to 1.86 Å resolution. The protein crystallized in a tetragonal space group (P4), with unit-cell parameter ...	2011	22139180
improvement of the redox balance increases l-valine production by corynebacterium glutamicum under oxygen deprivation conditions.	production of l-valine under oxygen deprivation conditions by corynebacterium glutamicum lacking the lactate dehydrogenase gene ldha and overexpressing the l-valine biosynthesis genes ilvbncde was repressed. this was attributed to imbalanced cofactor production and consumption in the overall l-valine synthesis pathway: two moles of nadh was generated and two moles of nadph was consumed per mole of l-valine produced from one mole of glucose. in order to solve this cofactor imbalance, the coenzyme ...	2011	22138982
bio-based production of chemicals, materials and fuels -corynebacterium glutamicum as versatile cell factory.	since their discovery almost 60 years ago, corynebacterium glutamicum and related subspecies are writing a remarkable success story in industrial biotechnology. today, these gram-positive soil bacteria, traditionally well-known as excellent producers of l-amino acids are becoming flexible, efficient production platforms for various chemicals, materials and fuels. this development is intensively driven by systems metabolic engineering concepts integrating systems biology and synthetic biology int ...	2011	22138494
Phenotypic characterization of Corynebacterium glutamicum using elementary modes towards synthesis of amino acids.	Elementary flux mode (EFM) analysis is a powerful tool to represent the metabolic network structure and can be further utilized for flux analysis. The method enables characterization and quantification of feasible phenotypes in microbes. EFM analysis was employed to characterize the phenotype of Corynebacterium glutamicum to yield various amino acids. The metabolic network of C. glutamicum yielded 62 elementary modes by incorporating the accumulation of amino acids namely, lysine, alanine, valin ...	2011	22132055
activity of daptomycin and three comparator agents against non-diphtheriae corynebacterium isolates of clinical interest.		2011	22129591
Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production.	The first biosynthetic system for lactate (LA)-based polyesters was previously created in recombinant Escherichia coli (Taguchi et al. 2008). Here, we have begun efforts to upgrade the prototype polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C. glutamicum to generate monomer substrates, lactyl-CoA (LA-CoA), and 3-hydroxybutyryl-CoA (3HB-CoA), for t ...	2011	22127753
Synthesis, characterization and antibacterial studies on some mixed ligand thorium complexes with N- and O-donor ligands.	Mixed ligand Th(IV) complexes of the type [M(Q)(L)(NO3)2] x 2H2O have been synthesized using 8-hydroxyquinoline (HQ) as a primary ligand and N- and/or O- donor amino acids (HL) such as L-threonine, L-tryptophan and L-isoleucine as secondary ligands. The metal complexes have been characterized on the basis of elemental analysis, electrical conductance, room temperature magnetic susceptibility measurements, spectral and thermal studies. The electrical conductance studies of the complexes in DMF in ...	2011	22125953
Corynebacterium ulcerans diphtheria: an emerging zoonosis in Brazil and worldwide.	The article is a literature review on the emergence of human infections caused by Corynebacterium ulcerans in many countries including Brazil. Articles in Medline/PubMed and SciELO databases published between 1926 and 2011 were reviewed, as well as articles and reports of the Brazilian Ministry of Health. It is presented a fast, cost-effective and easy to perform screening test for the presumptive diagnosis of C. ulcerans and C. diphtheriae infections in most Brazilian public and private laborat ...	2011	22124745
complete genome sequence of corynebacterium pseudotuberculosis strain cip 52.97, isolated from a horse in kenya.	in this work, we report the whole-genome sequence of corynebacterium pseudotuberculosis bv. equi strain cip 52.97 (collection institut pasteur), isolated in 1952 from a case of ulcerative lymphangitis in a kenyan horse, which has evidently caused significant losses to agribusiness. therefore, obtaining this genome will allow the detection of important targets for postgenomic studies, with the aim of minimizing problems caused by this microorganism.	2011	22123771
Human septicemia caused by Corynebacterium bovis: a case report and review of the literature.	We report a rare case of Corynebacterium bovis septicemia about a 65 year old man who received prolonged antibiotic treatment with colistin, imipenem and fosfomycin. We discuss in this work the circumstances of emergence of C. bovis infection through a literature review.	2011	22123577
biochemical disclosure of the mycolate outer membrane of corynebacterium glutamicum.	corynebacterineae is a specific sub-order of gram-positive bacteria that includes mycobacterium tuberculosis and corynebacterium glutamicum. their cell wall is composed of a heteropolymer of peptidoglycan (pg) linked to arabinogalactan (ag), which in turn, is covalently associated to an atypical outer membrane hereafter called mycomembrane (m). this latter structure has been visualized by cryoelectron microscopy of vitreous sections but its biochemical composition is still poorly defined, thereb ...	2011	22123248
Biofilm formation and microbial community analysis of the simulated river bioreactor for contaminated source water remediation.	BACKGROUND, AIM, AND SCOPE: The start-up pattern of biofilm remediation system affects the biofilm characteristics and operating performances. The objective of this study was to evaluate the performances of the contaminated source water remediation systems with different start-up patterns in view of the pollutants removal performances and microbial community succession. METHODS: The operating performances of four ...	2011	22120124
production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant corynebacterium glutamicum.	although cellulosic materials of plant origin are the most abundant utilizable biomass resource, the amino acid-producing organism corynebacterium glutamicum can not utilize these materials. here we report the engineering of a c. glutamicum strain expressing functional minicellulosomes containing chimeric endoglucanase e bound to minicbpa from clostridium cellulovorans that can hydrolyze cellulosic materials. the chimeric endoglucanase e consists of the endoglucanase e catalytic backbone of clos ...	2011	22112952
[corynebacterium pseudodiphtheriticum causing severe pneumonia in secondary immunoglobulin deficiency].	corynebacterium pseudodiphtheriticum is of increasing importance because of the rising number of immunocompromised patients. pneumonia, but also endocarditis, urinary tract infections or keratitis can be caused by this bacteria in case of immunosuppression. taking corynebacterium pseudodiphtheriticum into consideration as causitive agent provides for a fast onset of targeted antibiotic therapy.	2011	22109586
efflux permease cgacr3-1 of corynebacterium glutamicum is an arsenite-specific antiporter.	resistance to arsenite (as(iii)) by cells is generally accomplished by arsenite efflux permeases from acr3 or arsb unrelated families. we analyzed the function of three acr3 proteins from corynebacterium glutamicum, cgacr3-1, cgacr3-2, and cgacr3-3. cgacr3-1 conferred the highest level of as(iii) resistance and accumulation in vivo. cgacr3-1 was also the most active when everted membranes vesicles from escherichia coli or c. glutamicum mutants were assayed for efflux with different energy source ...	2012	22102279
Site-Directed Mutagenesis Studies on the L: -Arginine-Binding Sites of Feedback Inhibition in N-Acetyl-L: -glutamate Kinase (NAGK) from Corynebacterium glutamicum.	Arginine biosynthesis in Corynebacterium glutamicum proceeds via a pathway that is controlled by arginine through feedback inhibition of NAGK, the enzyme that converts N-acetyl-L: -glutamate (NAG) to N-acety-L: -glutamy-L: -phosphate. In this study, the gene argB encoding NAGK from C. glutamicum ATCC 13032 was site-directed, and the L: -arginine-binding sites of feedback inhibition in Cglu_NAGK are described. The N-helix and C-terminal residues were first deleted, and the results indicated that ...	2011	22101454
Construction of a novel sacB-based system for marker-free gene deletion in Corynebacterium glutamicum.	Bacillus subtilis sacB gene with its 463bp upstream region including its native promoter has been used for marker-free gene deletion in Corynebacterium glutamicum, but the role of this upstream region is not clear. In this study, it was demonstrated that the upstream region of sacB failed to efficiently promote its expression in C. glutamicum, and the native promoter of sacB is weak in C. glutamicum. The expression level of sacB under its native promoter in C. glutamicum is not high enough for c ...	2012	22100974
antimicrobial drug resistance in corynebacterium diphtheriae mitis.		2011	22099107
Corynebacterium glutamicum whcB, a stationary phase-specific regulatory gene.	The function of whcB, one of the four whiB homologues of Corynebacterium glutamicum, was assessed. Cells carrying the P(180) -whcB clone, and thus overexpressing the whcB gene, showed retarded growth, probably due to increased sensitivity to oxidants, whereas cells lacking whcB (?whcB) did not. However, growth retardation was not observed in cells with additionally whcE deleted. Furthermore, the ?whcE phenotype, characterized by slow growth and sensitivity to oxidants, was reversed in cells carr ...	2011	22098456
Bacteriology of hidradenitis suppurativa exacerbations. Deep tissue cultures obtained during carbon dioxide laser treatment.	Background: Previous studies have shown different bacteria in hidradenitis suppurativa (HS) lesions, but the literature regarding bacteria in acute exacerbation of HS is sparse. Objectives: The aim of the study was to determine the type of bacteria isolated from HS lesions during exacerbation of the disease. Methods: HS patients with acute nodules or abscesses were examined and treated by carbon dioxide laser vaporisation. Bacterial samples for aerobic and anaerobic cultures were taken from t ...	2011	22098253
corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products.	corynebacterium glutamicum, the industrial microbe traditionally used for the production of amino acids, proved its value for the fermentative production of diverse products through genetic/metabolic engineering. a successful demonstration of the heterologous expression of arabinose and xylose utilization genes made them interesting biocatalysts for pentose fermentation, which are the main components in lignocellulosic hydrolysates. its ability to withstand substantial amount of general growth i ...	2011	22094976
phenol degradation activity and reusability of corynebacterium glutamicum coated with nh(2)-functionalized silica-encapsulated fe(3)o(4) nanoparticles.	in this study, a novel method to immobilize and separate corynebacterium glutamicum for phenol degradation was developed using fe(3)o(4) nanoparticles (nps). the fe(3)o(4) nps were encapsulated with silica and functionalized with nh(2) groups to enhance their capacity to adsorb on the cell surface. the results showed that the nh(2)-functionalized silica-encapsulated fe(3)o(4) nps strongly adsorbed on the cell surface of c. glutamicum during 32d culture without any interruptions of their normal c ...	2012	22093979
Haptoglobin and fibrinogen concentrations and leukocyte counts in the clinical investigation of caseous lymphadenitis in sheep.	Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CLA), a disease that affects small ruminants and is responsible for economic losses, including condemnation of carcasses and damaged hides.	2011	22092668
Characterization and Comparison of Invasive Corynebacterium diphtheriae Isolates from France and Poland.	Corynebacterium diphtheriae, the agent of diphtheria, is rarely responsible for bacteremia. However, high numbers of bacteremia have been reported in countries with extensive immunization coverage. Here, we used molecular and phenotypic tools to characterize and compare 42 invasive isolates collected in France (including New Caledonia) and Poland over a 23-year period.	2012	22090411
Antibiotic susceptibility of Corynebacterium minutissimum isolated from lesions of Turkish patients with erythrasma.		2011	22082845
Corynebacterium epidermidicanis sp. nov., isolated from a dog's skin.	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the skin of a dog, designated strain 410T, was subjected to comprehensive taxonomic characterization. Comparison of the 16S rRNA gene sequence showed highest similarities to Corynebacterium diphtheriae, Corynebacterium pseudotuberculosis, and Corynebacterium ulcerans (96.1-96.7%). The quinone system consisted predominantly of MK-8(H2) and MK-9(H2). The polar lipid profile of strain 410T contained the major compounds d ...	2011	22081710
Urosepsis caused by Globicatella sanguinis and Corynebacterium riegelii in an adult: case report and literature review.	We report an extremely rare case of urosepsis caused by Globicatella sanguinis and Corynebacterium riegelii coinfection in a 94-year-old Japanese man with nephrolithiasis. Prompt identification of this coinfection is important so that effective antimicrobial coverage can be initiated.	2011	22080192
the effect of including lactobacillus reuteri kub-ac5 during post-hatch feeding on the growth and ileum microbiota of broiler chickens.	the probiotic strain lactobacillus reuteri kub-ac5, which was originally isolated from chicken intestine, was fed to newborn broiler chicks for the first week post-hatch. the growth and ileum microbiota of the chickens were carefully monitored for 6 wk. the inclusion of 5 log cfu/g of feed statistically increased the bw gain in the first week compared with that of the control group, but this effect did not continue thereafter. significant effects on host feed consumption and the feed-to-growth c ...	2011	22080014
evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations.	the aim of this study was to evaluate the specificity of the most widely used tuberculosis (tb) diagnostic tests, single intradermal tuberculin (sit) and single comparative intradermal tuberculin (scit) tests and interferon-gamma (ifn-γ) assay in 937 animals from eight tb-free caprine flocks under different epidemiological situations. maximum specificity was found using scit test (99.4-100% depending on the interpretation criteria) while sit test and ifn-γ assay showed a slightly lower overall s ...	2011	22079129
identification of non-diphtheriae corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.	we evaluated the bruker biotyper matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry for identification of 92 clinical isolates of corynebacterium species in comparison to identification using rpob or 16s rrna gene sequencing. eighty isolates (87%) yielded a score of ≥1.700, and all of these were correctly identified to the species level with the exception of corynebacterium aurimucosum being misidentified as the closely related corynebacterium minutissimum.	2012	22075579
[Drug sensitivity of causative agents in ocular infection of external adnexa and anterior segments--multicenter study of causative agents and drug sensitivity of ocular infection by the Japanese Association for Ocular Infection part II].	To report the drug sensitivity of causative agents produced by ocular infection of external adnexa and anterior segments investigated by the nationwide survey conducted by Japanese Association for Ocular Infection between September, 2007 and August, 2008.	2011	22073598
[determination of causative agents in ocular infection of external adnexa and anterior segments--multicenter study of causative agents and drug sensitivity of ocular infection by the japanese association for ocular infection part i].	to determine the causative agents of ocular infection of external adnexa and anterior segments due to selected criteria.	2011	22073597
high sero-prevalence of caseous lymphadenitis identified in slaughterhouse samples as a consequence of deficiencies in sheep farm management in the state of minas gerais, brazil.	abstract:	2011	22067701
Complete genome sequence of Corynebacterium variabile DSM 44702 isolated from the surface of smear-ripened cheeses and insights into cheese ripening and flavor generation.	Corynebacterium variabile is part of the complex microflora on the surface of smear-ripened cheeses and contributes to the development of flavor and textural properties during cheese ripening. Still little is known about the metabolic processes and microbial interactions during the production of smear-ripened cheeses. Therefore, the gene repertoire contributing to the lifestyle of the cheese isolate C. variabile DSM 44702 was deduced from the complete genome sequence to get a better understandin ...	2011	22053731
conformational changes of the betaine transporter betp from corynebacterium glutamicum studied by pulse epr spectroscopy.	the betaine transporter betp from corynebacterium glutamicum is activated by hyperosmotic stress critically depending on the presence and integrity of its sensory c-terminal domain. the conformational properties of the trimeric betp reconstituted in liposomes in the inactive state and during osmotic activation were investigated by site-directed spin labeling and electron paramagnetic resonance (epr) spectroscopy. comparison of intra- and intermolecular inter spin distance distributions obtained ...	2011	22051018
Physiology and global gene expression of a Corynebacterium glutamicum ?F(1)F(O)-ATP synthase mutant devoid of oxidative phosphorylation.	A mutant of Corynebacterium glutamicum ATCC 13032 with a deletion of the atpBEFHAGDC genes encoding F(1)F(O)-ATP synthase was characterized. Whereas no growth was observed with acetate as sole carbon source, the ?F(1)F(O) mutant reached 47% of the growth rate and 65% of the biomass of the wild type during shake-flask cultivation in glucose minimal medium. Initially, the mutant strain showed a strongly increased glucose uptake rate accompanied by a high oxygen consumption rate and pyruvate secret ...	2012	22050934
multiple pyogranulomas in a katahdin ewe.		2011	22043082
Inflammation regulates microRNA expression in cooperation with p53 and nitric oxide.	microRNA (miRNA) are small non-coding RNA targeting mRNAs leading to their instability and diminished translation. Altered expression of miRNA is associated with cancer. Inflammation and nitric oxide modulates the development of lymphomas in p53 knockout mice and there exists a negative feedback loop between p53 and NOS2. Using a genetic strategy, we tested the hypothesis that inflammation-induced oxidative and nitrosative stress modulates miRNA expression in mouse model deficient in either p53 ...	2011	22042537
human diphtheria infection from a cat bite.		2011	22041960
corynebacterium equi in cattle and pigs.	summary the cervical lymph nodes of pigs, the retropharyngeal and submandibular lymph nodes of cattle and faecal samples from both animal species were examined for the presence of corynebacterium equi. the organism was recovered from 19 (35 per cent) of 54 porcine cervical lymph nodes and from 0 of 54 bovine retropharyngeal and submandibular lymph nodes. fifteen (50 per cent) of 30 bovine faecal and 11 (35 per cent) of 31 porcine faecal samples yielded c. equi.	1980	22039855
whole-genome sequence of corynebacterium pseudotuberculosis pat10 strain isolated from sheep in patagonia, argentina.	in this work, we report the complete genome sequence of a corynebacterium pseudotuberculosis pat10 isolate, collected from a lung abscess in an argentine sheep in patagonia, whose pathogen also required an investigation of its pathogenesis. thus, the analysis of the genome sequence offers a means to better understanding of the molecular and genetic basis of virulence of this bacterium.	2011	22038974
Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms.	Arsenate reductases (ArsCs) evolved independently as a defence mechanism against toxic arsenate. In the genome of Corynebacterium glutamicum, there are two arsenic resistance operons (ars1 and ars2) and four potential genes coding for arsenate reductases (Cg_ArsC1, Cg_ArsC2, Cg_ArsC1' and Cg_ArsC4). Using knockout mutants, in vitro reconstitution of redox pathways, arsenic measurements and enzyme kinetics, we show that a single organism has two different classes of arsenate reductases. Cg_ArsC1 ...	2011	22032722
Diphtheria-like Illness due to Corynebacterium Ulcerans infection.		2011	22028605
role of bundle helices in a regulatory crosstalk in the trimeric betaine transporter betp.	the na(+)-coupled betaine symporter betp regulates transport activity in response to hyperosmotic stress only in its trimeric state, suggesting a regulatory crosstalk between individual protomers. betp shares the overall fold of two inverted structurally related five-transmembrane (tm) helix repeats with the sequence-unrelated na(+)-coupled symporters leut, vsglt, and mhp1, which are neither trimeric nor regulated in transport activity. conformational changes characteristic for this transporter ...	2011	22024596
Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum.	Corynebacterium glutamicum, an established industrial amino acid producer, has been genetically modified for efficient succinate production from the renewable carbon source glucose under fully aerobic conditions in minimal medium. The initial deletion of the succinate dehydrogenase genes (sdhCAB) led to an accumulation of 4.7 g l(-1) (40 mM) succinate as well as high amounts of acetate (125 mM) as by-product. By deleting genes for all known acetate-producing pathways (pta-ackA, pqo and cat) ace ...	2012	22018023
[Cloning, expression and characterization of N-acetylornithine aminotransferase from Corynebacterium crenatum and its effects on L-arginine fermentation].	N-Acetylornithine aminotransferase (EC 2.6.1.11, ACOAT) catalyzes the conversion of N-acetylglutamic semialdehyde to N-acetylornithine, the forth step involved in the L-arginine biosynthetic pathways. We studied the enzyme properties to set up reliable theoretical basis for the arginine fermentation optimization. ACOAT encoding gene argD was cloned from an industrial L-arginine producer Corynebacterium crenatum SYPA 5-5. Analysis of argD sequences revealed that only one ORF existed, which coded ...	2011	22016985
Etiology and epidemiological analysis of glaucoma-filtering bleb infections in a tertiary eye care hospital in South India.	To evaluate the microbial etiology and associated risk factors among patients with blebitis following trabeculectomy.	2011	22011488
Heterologous and homologous expression of the arginine biosynthetic argC~H cluster from Corynebacterium crenatum for improvement of L: -arginine production.	The genes involved in L: -arginine biosynthesis in Corynebacterium crenatum are organized as the argCJBDFRGH cluster like in Corynebacterium glutamicum. However, the argC~H cluster of the C. crenatum SYPA 5-5, which is an industrialized L: -arginine producer, had a lethal mutation occurring in the ArgR repressor encoding gene. The argC~H cluster with an inactive argR was overexpressed in E. coli and C. crenatum. In the recombinant E. coli JM109 enzyme activities were increased, and more L: -argi ...	2011	22009057
current knowledge on isobutanol production with escherichia coli, bacillus subtilis and corynebacterium glutamicum.	due to steadily rising crude oil prices great efforts have been made to develop designer bugs for the fermentative production of higher alcohols, such as 2-methyl-1-butanol, 3-methyl-1-butanol and 2-methyl-1-propanol (isobutanol), which all possess quality characteristics comparable to traditional oil based fuels. the common metabolic engineering approach uses the last two steps of the ehrlich pathway, catalyzed by 2-ketoacid decarboxylase and an alcohol dehydrogenase converting the branched cha ...	2011	22008938
Impact of a new glucose utilization pathway in amino acid-producing Corynebacterium glutamicum.	Corynebacterium glutamicum imports and phosphorylates glucose, fructose and sucrose by the phosphoenolpyruvate-dependent phosphotransferase carbohydrate uptake system (PTS). Recently, we have discovered how glucose can be utilized by C. glutamicum in a PTS-independent manner. PTS-independent glucose uptake is mediated by one of two inositol permeases (IolT1 or IolT2) and the second function of PTS, substrate phosphorylation, is catalyzed by one of two glucokinases (Glk or PpgK). PTS-deficient C. ...	2011	22008639
growth independent rhamnolipid production from glucose using the non-pathogenic pseudomonas putida kt2440.	abstract:	2011	21999513
genome sequence of corynebacterium glutamicum s9114, a strain for industrial production of glutamate.	here we report the genome sequence of corynebacterium glutamicum s9114, an industrial producer widely used in production of glutamate in china. preliminary comparison with the sequences of the corynebacterium glutamicum strains atcc 13032 and r revealed some notable mutagenesis that might be related to the high yield of glutamate.	2011	21994927
locating an extracellular k+-dependent interaction site that modulates betaine-binding of the na+-coupled betaine symporter betp.	betp, a trimeric na(+)-coupled betaine symporter, senses hyperosmotic stress via its cytoplasmic c-terminal domain and regulates transport activity in dependence of the cytoplasmic k(+)-concentration. this transport regulation of betp depends on a sophisticated interaction network. using single-molecule force spectroscopy we structurally localize and quantify these interactions changing on k(+)-dependent transport activation and substrate-binding. k(+) significantly strengthened all interactions ...	2011	21987793
deletion of the aconitase gene in corynebacterium glutamicum causes strong selection pressure for secondary mutations inactivating citrate synthase.	the aconitase gene acn of corynebacterium glutamicum is regulated by four transcriptional regulators, indicating that the synthesis of this enzyme is carefully controlled. to understand the causes for this elaborate regulation, the properties of the δacn-1 deletion mutant were analyzed in detail. the mutant was glutamate auxotrophic in glucose minimal medium, showed a strong growth defect, and secreted large amounts of acetate. none of these phenotypes could be complemented by plasmid-encoded ac ...	2011	21984793
The prevalence of interdigital erythrasma in southern region of Turkey.	Background Erythrasma is a skin infection which is caused by Corynebacterium minutissimum. Interdigital erythrasma is the most common form. Objective The aim of this study was to detect the frequency and risk factors of interdigital erythrasma in patients with clinically suspected tinea pedis. Methods This study was conducted between June and December 2010 and included 122 patients who had interdigital foot lesions. All patients were examined using a Wood's lamp. The smears were stained using ...	2011	21977942
structural view of the regulatory subunit of aspartate kinase from mycobacterium tuberculosis.	the aspartate kinase (ak) from mycobacterium tuberculosis (mtb) catalyzes the biosynthesis of aspartate family amino acids, including lysine, threonine, isoleucine and methionine. we determined the crystal structures of the regulatory subunit of aspartate kinase from mtb alone (referred to as mtbakβ) and in complex with threonine (referred to as mtbakβ-thr) at resolutions of 2.6 å and 2.0 å, respectively. mtbakβ is composed of two perpendicular non-equivalent act domains [aspartate kinase, chori ...	2011	21976064
genomic library screening for viruses from the human dental plaque revealed pathogen-specific lytic phage sequences.	bacterial pathogenesis presents an astounding arsenal of virulence factors that allow them to conquer many different niches throughout the course of infection. principally fascinating is the fact that some bacterial species are able to induce different diseases by expression of different combinations of virulence factors. nevertheless, studies aiming at screening for the presence of bacteriophages in humans have been limited. such screening procedures would eventually lead to identification of p ...	2012	21969025
indigenous soil bacteria with the combined potential for hydrocarbon consumption and heavy metal resistance.	introduction: transconjugant bacteria with combined potential for hydrocarbon utilization and heavy metal resistance were suggested by earlier investigators for bioremediation of soils co-contaminated with hydrocarbons and heavy metals. the purpose of this study was to offer evidence that such microorganisms are already part of the indigenous soil microflora. methods: microorganisms in pristine and oily soils wer ...	2011	21948132
rapid identification of bovine mastitis pathogens by high-resolution melt analysis of 16s rdna sequences.	accurate identification of mastitis pathogens is often compromised when using conventional culture-based methods. here, we report a novel, rapid assay tested for speciation of bacterial mastitis pathogens using high-resolution melt analysis (hrma) of 16s rdna sequences. real-time pcr amplification of 16s rrna gene fragment, spanning the variable region v5 and v6 was performed with a resulting amplicon of 290bp. first, a library was generated of melt curves of 9 common pathogens that are implicat ...	2011	21944716
the effect of recurrent episodes of clinical mastitis caused by gram-positive and gram-negative bacteria and other organisms on mortality and culling in holstein dairy cows.	the objective of this study was to estimate the effects of recurrent episodes of different types of clinical mastitis (cm) caused by gram-positive (streptococcus spp., staphylococcus aureus, staphylococcus spp.) and gram-negative (escherichia coli, klebsiella, citrobacter, enterobacter, pseudomonas) bacteria, and other organisms (arcanobacterium pyogenes, mycoplasma, corynebacterium bovis, yeast, miscellaneous) on the probability of mortality and culling in holstein dairy cows. data from 30,233 ...	2011	21943738
[pitted keratolysis--how to treat?].	pitted keratolysis is a common and problematic skin condition affecting the feet caused by the keratolytic activity of corynebacterium, micrococcus, dermatophilus. skin lesions are characterized by crater-like pits of the epidermis accompanied by a burning sensation, itching, pain and malodor. the purpose of the present paper was to characterise the disease's clinical features and possibilities for the therapy. due to its interdisciplinary nature, not only dermatologists ought to be familiar wit ...	2011	21936353
[A frequently overlooked bacteria in clinical microbiology laboratories: arcanobacterium haemolyticum].	Arcanobacterium haemolyticum, previously known as Corynebacterium haemolyticum, is a facultative anaerobic, gram-positive bacillus with negative catalase and positive CAMP inhibition test results. It may be the causative agent of about 0.5-3% of acute bacterial pharyngitis especially in children and young adults. Since growth of A.haemolyticum is usually inhibited by flora members and since it slowly develops hemolysis in sheep blood agar and its colony morphology resembles beta-hemolytic strept ...	2011	21935787
molecular characterization of prpr, the transcriptional activator of propionate catabolism in corynebacterium glutamicum.	the 2-methylcitrate cycle is used to metabolize propionate in corynebacterium glutamicum. the regulator, prpr (cg0800), of the prpdbc2 operon was identified and characterized. the regulator has no similarities to the up to now known prpr regulators from other organisms. growth of a δprpr mutant revealed severe growth deficits and a prolonged lag phase if propionate was present in the medium. transcriptome analyses demonstrated the inability of the δprpr strain to induce the prpdbc2 genes in the ...	2011	21933687
Structure of a highly NADP+-specific isocitrate dehydrogenase.	Isocitrate dehydrogenase catalyzes the first oxidative and decarboxylation steps in the citric acid cycle. It also lies at a crucial bifurcation point between CO2-generating steps in the cycle and carbon-conserving steps in the glyoxylate bypass. Hence, the enzyme is a focus of regulation. The bacterial enzyme is typically dependent on the coenzyme nicotinamide adenine dinucleotide phosphate. The monomeric enzyme from Corynebacterium glutamicum is highly specific towards this coenzyme and the su ...	2011	21931217
Prevalence of Corynebacterium ulcerans in dogs residing in Osaka, Japan.	Diphtheria-like human illness caused by Corynebacterium ulcerans is an emerging threat in developed countries, with incidence sometimes higher than that of diphtheria caused by Corynebacterium diphtheriae. Companion animals are considered a potential source of human infections. In order to determine the prevalence of C. ulcerans among dogs, we performed a screening for the bacterium in 583 dogs in the custody of the Osaka Prefectural government. Forty-four dogs (7.5%) were positive for the bacte ...	2011	21921111
Characterization of Corynebacterium diphtheriae isolates from infected skin lesions in the Northern Territory of Australia.	Corynebacterium diphtheriae is commonly isolated from cutaneous skin lesions in the Northern Territory of Australia. We prospectively assessed 32 recent isolates from infected skin lesions, in addition to reviewing 192 isolates collected over 5 years for toxin status. No isolates carried the toxin gene. Toxigenic C. diphtheriae is now a rare occurrence in the Northern Territory.	2011	21918025
[Genotyping and molecular marking of bacteria and viruses in epidemiological surveillance of actual infections].	Determination of genetic and molecular features of pathogens circulating in Russia, in the northwest of the country and in St. Petersburg to resolve the problems of spread of diseases caused by these pathogens.	2011	21913388
Ethambutol-mediated cell wall modification in recombinant Corynebacterium glutamicum increases the biotransformation rates of cyclohexanone derivatives.	The effects of structural modification of cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells, expressing the chnB gene encoding cyclohexanone monooxygenase of Acinetobacter calcoaceticus NCIMB 9871, were investigated. Baeyer-Villiger oxygenation of 2-(2'-acetoxyethyl) cyclohexanone (MW 170 Da) into R-7-(2'-acetoxyethyl)-2-oxepanone was used as a model reaction. The whole-cell biotransformation followed Michaelis-Menten kinetics. The V (max) and K (S) va ...	2011	21909677
bacterial classification using genomic fingerprints obtained by virtual hybridization.	in silico genomic fingerprints were produced by virtual hybridization of 191 fully sequenced bacterial genomes using a set of 15,264 13-mer probes specially designed to produce universal whole genome fingerprints. a novel approach for constructing phylogenetic trees, based on comparative analysis of genomic fingerprints, was developed. the resultant bacterial phylogenetic tree had strong similarities to those produced from the alignment of conserved sequences. notably, the trees derived from the ...	2011	21906634
a disarticulating spinal lesion. bacterial abscess.		2011	21905296
The glgB-encoded glycogen branching enzyme is essential for glycogen accumulation in Corynebacterium glutamicum.	Corynebacterium glutamicum transiently accumulates glycogen as carbon capacitor during the early exponential growth phase in media containing carbohydrates. In some bacteria glycogen is synthesized by the consecutive action of ADP-glucose pyrophosphorylase (GlgC), glycogen synthase (GlgA) and glycogen branching enzyme (GlgB). GlgC and GlgA of C. glutamicum have been shown to be necessary for glycogen accumulation in this organism. However, although cg1381 has been annotated as the putative C. gl ...	2011	21903753
Improving protein secretion of a transglutaminase-secreting Corynebacterium glutamicum recombinant strain on the basis of (13)C metabolic flux analysis.	Corynebacterium glutamicum is known as a host species for amino acid production. This microorganism was recently noticed as a host that produces secreted proteins. In this study, we performed (13)C metabolic flux analysis ((13)C-MFA) on a recombinant C. glutamicum strain that secretes a heterologous transglutaminase (TGase) to improve TGase secretion. For the (13)C-MFA of a TGase-secreting C. glutamicum strain in batch cultivation, a (13)C-labeling experiment and measurement of mass isotopomer d ...	2011	21903468
site-directed mutagenesis and feedback-resistant n-acetyl-l: -glutamate kinase (nagk) increase corynebacterium crenatum l: -arginine production.	n-acetyl-l: -glutamate kinase (ec 2.7.2.8) is first committed in the specific l: -arginine pathway of corynebacterium sp. a limited increase of l: -arginine production for the argb overexpression in the engineering c. creantum sypa-ccb strain indicated that l: -arginine feedback inhibition plays an influence on the l: -arginine production. in this study, we have performed site-directed mutagenesis of the key enzyme (nagk) and the three mutations (e19r, h26e and h268d) exhibited the increase of i ...	2011	21901472
[monoclonal antibodies labeled with colloidal gold for immunochromatographic express analysis of diphtheria toxin].	one-step rapid immunochromatographic method for detection of diphtheria toxin in different water samples (phosphate buffer, milk, human nasopharyngeal swab) with the conjugate of monoclonal antibodies labeled with colloidal gold was developed. the limit of visible detection of the diphtheria toxin is 10 ng/ml and 15 min time analysis. the use of silver sensitivity enhancement and scanning equipment decreased the detection limit to 1.25 ng/ml.	2011	21899052
photoletter to the editor: trichomycosis (trichobacteriosis) axillaris.	trichomycosis axillaris is a common tropical disease usually affecting the hair shafts of the axillae, characterized by nodular concretions along the hair shafts caused by corynebacterium tenuis. we describe a 38-year-old patient with trichomycosis axillaris. treatment, which included shaving of affected hair, followed by topical 3% erythromycin cream and clotrimazole powder was fully effective.	2011	21894255
Overexpression and purification of the recombinant diphtheria toxin variant CRM197 in Escherichia coli.	The expression of the recombinant diphtheria toxin mutant CRM197 in bacteria other than Corynebacterium diphtheriae has proven to be difficult. Here we propose a new and alternative procedure for the production of full-length CRM197 in Escherichia coli. The present study relates specifically to the expression of an artificial sequence and to a method for the isolation and purification of the corresponding protein. In particular, a synthetic gene coding for CRM197, bearing a short histidine tag a ...	2011	21889551