Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| cloning of inulin fructotransferase (dfa iii-producing) gene from arthrobacter sp. l68-1. | a gene of inulin fructotransferase (dfa iii-producing) [ec 2.4.1.93] from arthrobacter sp. l68-1 was cloned and the nucleotide was sequenced. the gene encoded a signal peptide (32 amino acid residues) for a secretion, and the mature enzyme protein was estimated to be consisted with 410 amino acid residues. the molecular mass of the native enzyme was calculated as 43.7kda by the sequence data. the deduced amino acid sequence of the enzyme had 79.0% homology with that of the arthrobacter globiform ... | 2012 | 23499085 |
| the paax-type repressor meqr2 of arthrobacter sp. strain rue61a, involved in the regulation of quinaldine catabolism, binds to its own promoter and to catabolic promoters and specifically responds to anthraniloyl coenzyme a. | the genes coding for quinaldine catabolism in arthrobacter sp. strain rue61a are clustered on the linear plasmid pal1 in two upper pathway operons (meqabc and meqdef) coding for quinaldine conversion to anthranilate and a lower pathway operon encoding anthranilate degradation via coenzyme a (coa) thioester intermediates. the meqr2 gene, located immediately downstream of the catabolic genes, codes for a paax-type transcriptional repressor. meqr2, purified as recombinant fusion protein, forms a di ... | 2012 | 23275246 |
| production of cyclic adenosine monophosphate by arthrobacter sp. a302 using fed-batch fermentation with ph-shift control. | the production of cyclic adenosine monophosphate (camp) by arthrobacter sp. a302 was studied in a 5 l stirred tank fermentor under a range of ph values (6.5-8.0) and glucose feeding rates. in batch fermentation under a controlled ph, the optimum ph for cell growth was 7.5 with dry cell density (x) of 11.43 g l, and the optimum ph for camp accumulation was 7.0 with camp concentration of 7.41 g l. in order to achieve the high x and camp yield simultaneously, a ph-shift control strategy was propose ... | 2011 | 22806787 |
| study on the flocculability of the arthrobacter sp., an actinomycete resuscitated from the vbnc state. | a bioflocculant with high flocculating activity, lc13-sf, produced by strain lc13(t) which was in a viable but nonculturable (vbnc) state, and which was woken up by rpf (resuscitation promoting factor), was systematically investigated with regard to its fermentation conditions and flocculating activity. the key parameters influencing the bioflocculant lc13-sf were investigated through measuring the optical density at 660 (od(660)) of the fermentation liquid and the optical density at 550 (od(550 ... | 2011 | 22806783 |
| chemotaxis to atrazine and detection of a xenobiotic catabolic plasmid in arthrobacter sp. dns10. | a plasmid named pdns10 was detected from an atrazine-degrading strain arthrobacter sp. dns10 which has been isolated previously in our laboratory. | 2011 | 22351258 |
| [effect of cellulose-decomposing strain on microbial community of cow manure compost]. | taking the cow dung and straw as composting raw materials, effect of cellulose-decomposing strain on microbial community of cow manure compost was investigated with the traditional culture method and pcr-dgge technique. the results showed that the microbiological inocula showed a more rapid rate of temperature elevation at the start of composting and prolonged the time of high-temperature process and increased the number of microbial. the dgge map of cellulose-decomposing strain compost was diff ... | 2011 | 22279926 |
| the three-dimensional structure of nylon hydrolase and the mechanism of nylon-6 hydrolysis. | we performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (nylc) from agromyces sp. at 2.0 å-resolution. this enzyme is a member of the n-terminal nucleophile (n-tn) hydrolase superfamily that is responsible for the degradation of the nylon-6 industry byproduct. we observed four identical heterodimers (27kda+9kda), which resulted from the autoprocessing of the precursor protein (36kda) and which constitute the doughnut-shaped quaternary structure. the catalytic resi ... | 2011 | 22187439 |
| impact of pgpr inoculation on growth and antioxidant status of wheat under saline conditions. | two plant growth-promoting rhizobacterial (pgpr) strains, bacillus subtilis su47 and arthrobacter sp. su18, were found to tolerate 8% nacl. wheat co-inoculated with these two pgpr strains, and grown under different salinity regimes (2-6 ds m(-1) ), showed an increase in dry biomass, total soluble sugars and proline content. wheat sodium content was reduced under co-inoculated conditions but not after single inoculation with either strain or in the control. the activity of antioxidant enzymes in ... | 2011 | 22136617 |
| an isofenphos-methyl hydrolase (imh) capable of hydrolyzing the p-o-z moiety of organophosphorus pesticides containing an aryl or heterocyclic group. | organophosphorus pesticide (op) hydrolases play key roles in the degradation and decontamination of agricultural and household ops and in the detoxification of chemical warfare agents. in this study, an isofenphos-methyl hydrolase gene (imh) was cloned from the isocarbophos-degrading strain of arthrobacter sp. scl-2 using the polymerase chain reaction method. isofenphos-methyl hydrolase (imh) showed 98% sequence identity with the isofenphos hydrolase from arthrobacter sp. strain b-5. imh was hig ... | 2011 | 22120622 |
| Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy. | Halohydrin dehalogenases are attractive biocatalysts in producing a series of important chiral building blocks. Recombinant expression of halohydrin dehalogenase from Arthrobacter sp. AD2 (HheA) in Escherichia coli using T7 promoter-based pGEF(+) system revealed much lower expression level than that of the well-studied halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC). In this study, we changed the codon usage in the 5'-end of hheA gene to improve the expression yield of HheA. Ou ... | 2011 | 22112566 |
| construction of escherichia coli-arthrobacter-rhodococcus shuttle vectors based on a cryptic plasmid from arthrobacter rhombi and investigation of their application for functional screening. | a cryptic plasmid from arthrobacter rhombi prh1, designated as pprh, was sequenced and characterized. it was 5000 bp in length with a g+c content of 66 mol%. the plasmid pprh was predicted to encode six putative open reading frames (orfs), in which orf2 and orf3 formed the minimal replicon of plasmid pprh and shared 55-61% and 60-69% homology, respectively, with the repa and repb proteins of reported rhodococcal plasmids. sequence analysis revealed a typical cole2-type ori located 45 bp upstream ... | 2011 | 22098420 |
| production of xylanase from arthrobacter sp. mtcc 6915 using saw dust as substrate under solid state fermentation. | saw dust was used as substrate for xylanase production from arthrobacter sp. mtcc 6915. the study of period of incubation, temperature, ph, carbon, and nitrogen sources for xylanase production was optimized. xylanase production was found to be optimum at an incubation period of 96 hrs (117.0 u/ml), temperature 30°c (105.0 u/ml), and ph 9.0 (102.9 u/ml). the results showed that the xylanase production was found to be higher in the presence of carboxymethylcellulose (176.4 u/ml) and dextrose (126. ... | 2011 | 22013512 |
| a novel transaminase, (r)-amine:pyruvate aminotransferase, from arthrobacter sp. knk168 (ferm bp-5228): purification, characterization, and gene cloning. | a novel (r)-amine transaminase, which catalyzed (r)-enantioselective transamination of chiral amine, was purified to homogeneity from arthrobacter sp. knk168 (ferm bp-5228). the molecular mass of the enzyme was estimated to be 148 kda by gel filtration and 37 kda by sodium dodecyl sulfate polyacrylamide gel electrophoresis, suggesting a homotetrameric structure. the enzyme catalyzed transamination between amines and pyruvate stereo-specifically. the reaction on 1-methylbenzylamine was (r)-enanti ... | 2011 | 22002066 |
| pathway for degradation of 2-chloro-4-nitrophenol in arthrobacter sp. sjcon. | degradation of 2-chloro-4-nitrophenol (2c4np) was studied by arthrobacter sp. sjcon, isolated from the soil of a pesticide contaminated site. this strain utilized 2c4np as sole source of carbon and energy and degraded 2c4np with stoichiometric release of nitrite and chloride ions. a metabolite was detected during the study of 2c4np degradation and identified as chlorohydroquinone (chq) by thin layer chromatography (tlc), high performance liquid chromatography (hplc), and gas chromatography-mass ... | 2011 | 21960016 |
| complete detoxification of tris(1,3-dichloro-2-propyl) phosphate by mixed two bacteria, sphingobium sp. strain tcm1 and arthrobacter sp. strain py1. | tris(1,3-dichloro-2-propyl) phosphate (tdcpp), a flame retardant, is regarded as a potentially toxic and persistent environmental contaminant. we previously isolated a tdcpp-degrading bacterium, sphingobium sp. strain tcm1, which, however, produced a toxic metabolite: 1,3-dichloro-2-propanol (1,3-dcp). this study was undertaken to develop a technique for complete tdcpp detoxification using strain tcm1 with a 1,3-dcp-degrading bacterium, arthrobacter sp. strain py1. for efficient detoxification, ... | 2011 | 21956155 |
| [study on 2-methylpyridine initial catabolic pathways in arthrobacter sp. km-2mp strain]. | 2011 | 21861369 | |
| [High throughput screening atrazine chlorohydrolase mutants with enhanced activity through Haematococcus pluvialis expression system]. | Developing a high-throughput screening method is of great importance for directed evolution of atrazine chlorohydrolase. A mutagenesis library of atzA from Pseudomonas sp. ADP and Arthrobacter sp. AD1 was constructed using error-prone PCR and DNA shuffling. Candidate mutants were screened through Haematococcus pluvialis expression system, using atrazine as selection pressure. Sequence analysis showed that mutations in the obtained 12 mutants with enhanced activity were all point-substitutions an ... | 2011 | 21847998 |
| a multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion. | this study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (fragaria ananassa) fruit. a total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-pcr banding pattern and biochemical features. phylogenetic analysis of the 16s rrna gene sequences of 45 representatives showed that the isolates belonged to t ... | 2011 | 21837472 |
| phylogenetic perspectives of nitrogen-fixing actinobacteria. | it was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus frankia. recently, the number of nitrogen fixation (nifh) genes identified in other non-frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. during the last decade, mycobacterium flavum, corynebacterium autotrophicum and a fluorescent arthr ... | 2011 | 21779790 |
| biodegradation kinetics of 4-fluorocinnamic acid by a consortium of arthrobacter and ralstonia strains. | arthrobacter sp. strain g1 is able to grow on 4-fluorocinnamic acid (4-fca) as sole carbon source. the organism converts 4-fca into 4-fluorobenzoic acid (4-fba) and utilizes the two-carbon side-chain for growth with some formation of 4-fluoroacetophenone as a dead-end side product. we also have isolated ralstonia sp. strain h1, an organism that degrades 4-fba. a consortium of strains g1 and h1 degraded 4-fca with monod kinetics during growth in batch and continuous cultures. specific growth rate ... | 2011 | 21728015 |
| screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey. | several microorganisms were isolated from soil/sediment samples of antarctic peninsula. the enrichment technique using (rs)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. we also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (rs)-1-(phenyl)ethanol as substrates. among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze th ... | 2011 | 21673897 |
| enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from arthrobacter sp. | in human serum, as for phospholipids not containing choline, phosphatidylethanolamine (pe) exists approximately 5% in a whole phospholipid. pe is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis and cell signaling. however, it could not measure pe with other phospholipids due to a lack of choline in them. | 2011 | 21549106 |
| molecular characterization of cold-inducible beta-galactosidase from arthrobacter sp. on14 isolated from antarctica. | a psychrotrophic bacterium, arthrobacter sp. on14, isolated from antarctica, was shown to exhibit a high beta-galactosidase activity at a low temperature. a genomic library of on14 was constructed and screened for beta-galactosidase genes on functional plates containing 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (x-gal) as the substrate. two different beta-galactosidase genes, named as gala, galb, were found in on14. computational analyses of the genes revealed that the encoded protein ... | 2011 | 21464592 |
| 2,4-d metabolism: pathway of degradation of chlorocatechols by arthrobacter sp. | 1969 | 21428341 | |
| genomic heterogeneity within conservedmetabolic pathways of arthrobacter species - a bioinformatic approach. | a comparative genomic analysis of three species of the soil bacterium arthrobacter was undertaken with specific emphasis on genes involved in important and core energy metabolism pathways like glycolysis and amino acid metabolism. during the course of this study, it was revealed that codon bias of a particular species, namely arthrobacter aurescens tc1, is significantly lower than that of the other two species a. chlorophenolicus a6 and arthrobacter sp. fb24. the codon bias was also found to be ... | 2011 | 21423891 |
| [isolation, identification and soil remediation of atrazine-degrading strain t3 ab1]. | to provide new atrazine-degrading strains for atrazine-polluted soil, we isolated the high-efficiency degradation bacterium from contaminated soil, identified with taxonomy, and studied the degrading characteristics and remediation capability of the strain in black soil. | 2010 | 21365918 |
| discovery and characterization of d-phenylserine deaminase from arthrobacter sp. tks1. | we discovered a d-phenylserine deaminase that catalyzed the pyridoxal 5'-phosphate (plp)-dependent deamination reaction from d-threo-phenylserine to phenylpyruvate in newly isolated arthrobacter sp. tks1. the enzyme was partially purified, and its n-terminal amino acid sequence was analyzed. based on the sequence information, the gene encoding the enzyme was identified and expressed in escherichia coli. the expressed protein was purified to homogeneity and characterized. the enzyme consisted of ... | 2010 | 21190106 |
| the arthrobacter arilaitensis re117 genome sequence reveals its genetic adaptation to the surface of cheese. | arthrobacter arilaitensis is one of the major bacterial species found at the surface of cheeses, especially in smear-ripened cheeses, where it contributes to the typical colour, flavour and texture properties of the final product. the a. arilaitensis re117 genome is composed of a 3,859,257 bp chromosome and two plasmids of 50,407 and 8,528 bp. the chromosome shares large regions of synteny with the chromosomes of three environmental arthrobacter strains for which genome sequences are available: ... | 2010 | 21124797 |
| degradative potential of stenotrophomonas strain hpc383 having genes homologous to dmp operon. | a strain, stenotrophomonas hpc383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through hplc and gc analysis. culture hpc383 could degrade (%) various compounds (1 mm) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% c ... | 2010 | 21123060 |
| lactulose biosynthesis by β-galactosidase from a newly isolated arthrobacter sp. | lactulose, a ketose disaccharide, is used in both pharmaceutical and food industries. this study was undertaken to screen and isolate potent β-galactosidase-producing bacteria and to evaluate their enzymatic production of lactulose. soil samples from fruit gardens were collected. one isolate designated las was identified whose cell extract could convert lactose and fructose into lactulose. the 16s rdna gene analysis of las revealed its phylogenetic relatedness to arthrobacter sp. the β-galactosi ... | 2010 | 21104424 |
| complete biodegradation of 4-fluorocinnamic acid by a consortium comprising arthrobacter sp. strain g1 and ralstonia sp. strain h1. | a consortium of the newly isolated bacterial strains arthrobacter sp. strain g1 and ralstonia sp. strain h1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. strain g1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some formation of 4-fluoroacetophenone as a dead-end side product. in the presence of strain h1, complete mineralization of 4-fluorocinnamic acid and release of fluoride were obtained. degradation of 4-fl ... | 2010 | 21097599 |
| root exudates modify bacterial diversity of phenanthrene degraders in pah-polluted soil but not phenanthrene degradation rates. | to determine whether the diversity of phenanthrene-degrading bacteria in an aged polycyclic aromatic hydrocarbon (pah) contaminated soil is affected by the addition of plant root exudates, dna stable isotope probing (sip) was used. microcosms of soil with and without addition of ryegrass exudates and with ¹³c-labelled phenanthrene (phe) were monitored over 12 days. phe degradation was slightly delayed in the presence of added exudate after 4 days of incubation. after 12 days, 68% of added phe di ... | 2010 | 21087382 |
| optimization of electroporation conditions for arthrobacter with plasmid part2. | a prerequisite for genetic studies of arthrobacter is a high efficiency transformation system that allows for dna transfer, transposon mutagenesis, and expression of specific genes. in this study, we develop a detailed electroporation method through a systematic examination of the factors involved in the entire electroporation process. key features of this procedure, including the addition of penicillin to cells during the early log phase of growth and the presence of 0.5m sorbitol in the electr ... | 2010 | 21078345 |
| identification of chitinases is-chia and is-chib from isoptericola jiangsuensis clg and their characterization. | a 274-bp conserved fragment of chia (chia-cf) was amplified from the genomic dna of isoptericola jiangsuensis clg (dsm 21863, cctcc ab208287) using the specific pcr primers. based on chia-cf sequences, a 5233-bp dna fragment was obtained by self-formed adaptor pcr. dna sequencing analysis revealed there were two contiguous open reading frames coding for the precursors of is-chia [871 amino acids (aa)] and is-chib (561 aa) in the 5233-bp dna fragment. the is-chia and is-chib exhibited 58% and 62% ... | 2010 | 20922373 |
| the catalytic efficiency of trehalose-6-phosphate synthase is effected by the n-loop at low temperatures. | the enzyme otsa (trehalose-6-phosphate synthase) is ubiquitous in both prokaryotic and eukaryotic organisms, where it plays a critical role in stress resistance and glucose metabolism. here, we cloned the otsa gene from arthrobacter sp. cjts, and expressed and then purified the recombinant proteins. enzyme activity analysis indicated that the high catalytic efficiency of otsa from arthrobacter sp. cjts resulted from the high affinity of the enzyme for uridine 5'-diphosphoglucose (udp-glc) at low ... | 2010 | 20838774 |
| phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal. | the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ... | 2010 | 20833430 |
| isolation and characterization of a pseudomonas sp. strain iitr01 capable of degrading α-endosulfan and endosulfan sulfate. | to isolate bacteria capable of degrading endosulfan (es) and the more toxic es sulfate and to characterize their metabolites. | 2010 | 20825519 |
| isolation and characterization of an arthrobacter sp. strain hb-5 that transforms atrazine. | a bacterial strain (hb-5) capable of utilizing atrazine as sole carbon and nitrogen source for growth was isolated from an industrial wastewater sample by enrichment culture. the isolate was identified as arthrobacter sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. the strain exhibited faster atrazine degradation rates in atrazine-containing mineral media than the well-characterized atrazine-degrading bacteria pseudomonas sp. adp. ... | 2011 | 20686824 |
| air-cathode microbial fuel cell array: a device for identifying and characterizing electrochemically active microbes. | microbial fuel cells (mfcs) have generated excitement in environmental and bioenergy communities due to their potential for coupling wastewater treatment with energy generation and powering diverse devices. the pursuit of strategies such as improving microbial cultivation practices and optimizing mfc devices has increased power generating capacities of mfcs. however, surprisingly few microbial species with electrochemical activity in mfcs have been identified because current devices do not suppo ... | 2011 | 20655725 |
| study on the removal of indoor vocs using biotechnology. | with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ... | 2010 | 20598440 |
| high-density polyethylene (hdpe)-degrading potential bacteria from marine ecosystem of gulf of mannar, india. | assessment of high-density polyethylene (hdpe)-degrading bacteria isolated from plastic waste dumpsites of gulf of mannar. | 2010 | 20586938 |
| influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments. | because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ... | 2010 | 20553043 |
| weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution. | several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ... | 2010 | 20525872 |
| homology modeling and docking study of xanthine oxidase of arthrobacter sp. xl26. | hyperuricemia is a condition of defective purine metabolism characterized with elevated serum uric acid (ua) level that further leads to gout and gouty nephrolithiasis disorders. gout is a world wide distributed rheumatic disease comprises 1% of the total population and still is in increasing state. one of the factors contributing to overproduction of ua is the hydroxylation of xanthine catalyzed by xanthine oxidase (xo). in the present study, 3d modeling of arthrobacter sp. xl26 (xodb) protein ... | 2010 | 20403378 |
| comparative study of properties of immobilized lipase onto glutaraldehyde-activated amino-silica gel via different methods. | the enzyme-aggregate coating method was performed to immobilize arthrobacter sp. lipase in order to achieve better catalytic properties comparable to the conventional covalent attachment and covalent attachment plus cross-linking. the glutaraldehyde-activated amino-silica gel which was synthesized by sol-gel technique was used as the support, and the catalytic characteristics of the lipase preparations were tested in the asymmetric acylation of 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-o ... | 2010 | 20399626 |
| cg2091 encodes a polyphosphate/atp-dependent glucokinase of corynebacterium glutamicum. | the corynebacterium glutamicum gene cg2091 is encoding a polyphosphate (polyp)/atp-dependent glucokinase (ppgk). previous work demonstrated the association of ppgk to polyp granules. the deduced amino acid sequence of ppgk shows 45% sequence identity to polyp/atp glucomannokinase of arthrobacter sp. strain km and 50% sequence identity to polyp glucokinase of mycobacterium tuberculosis h37rv. ppgk from c. glutamicum was purified from recombinant escherichia coli. polyp was highly preferred over a ... | 2010 | 20379711 |
| arthrobacter sp. strain ku001 isolated from a thai soil degrades atrazine in the presence of inorganic nitrogen sources. | an atrazine degrading bacterium, strain ku001, was obtained from a sugarcane field at the cane and sugar research and development center at the kasetsart university, kamphaeng saen campus, thailand. strain ku001 had a rod-to-coccus morphological cycle during growth. biolog carbon source analysis indicated that the isolated bacterium was arthrobacter histidinolovorans. sequence analysis of pcr product indicated the 16s rrna gene in strain ku001 was 99% identical to the same region in arthrobacter ... | 2010 | 20372034 |
| strains of internal biofilm in aerobic granular membrane bioreactors. | this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ... | 2010 | 20306189 |
| the biomass yielding process of xenobiotic degradation. | yields of activated sludge and an arthrobacter sp. biomass on organic xenobiotic 2,4-dichlorophenoxyacetic acid (2,4-d) and on the intermediates of selected 2,4-d metabolism pathways were measured. activated sludge yield on 2,4-d was lower by approximately 24-45% compared to the combined yields produced separately by the lower intermediates. for activated sludge, cell synthesis only consumed 33% of the electrons generated from 2,4-d oxidation, while the other 67% were used for energy. the high e ... | 2010 | 20153175 |
| influence of internal biofilm growth on residual permeability loss in aerobic granular membrane bioreactors. | membrane fouling results in flux decline or transmembrane pressure drop increase during membrane bioreactor (mbr) operation. physical and chemical cleanings are essential to keep an mbr operating at an appropriate membrane flux. considerable residual membrane permeability loss that cannot be removed by conventional cleaning requires membrane replacement. this study demonstrates that an internal biofilm can develop inside a hollow-fiber membrane and can probably account for up to 58.9 and 81.3% o ... | 2010 | 20102183 |
| growth of arthrobacter sp. strain jbh1 on nitroglycerin as the sole source of carbon and nitrogen. | arthrobacter sp. strain jbh1 was isolated from nitroglycerin-contaminated soil by selective enrichment. detection of transient intermediates and simultaneous adaptation studies with potential intermediates indicated that the degradation pathway involves the conversion of nitroglycerin to glycerol via 1,2-dinitroglycerin and 1-mononitroglycerin, with concomitant release of nitrite. glycerol then serves as the source of carbon and energy. | 2010 | 20061454 |
| heavy metal resistance in arthrobacter ramosus strain g2 isolated from mercuric salt-contaminated soil. | present study describes isolation of a multiple metal-resistant arthrobacter ramosus strain from mercuric salt-contaminated soil. the isolate was found to resist and bioaccumulate several metals, such as cadmium, cobalt, zinc, chromium and mercury. maximum tolerated concentrations for above metals were found to be 37, 525, 348, 1530 and 369 microm, respectively. the isolate could also reduce and detoxify redox-active metals like chromium and mercury, indicating that it has great potential in bio ... | 2010 | 20060643 |
| isolation of an isocarbophos-degrading strain of arthrobacter sp. scl-2 and identification of the degradation pathway. | isocarbophos is a widely used organophosphorus insecticide that has caused environmental pollution in many areas. however, degradation of isocarbophos by pure cultures has not been extensively studied, and the degradation pathway has not been determined. in this paper, a highly effective isocarbophos-degrading strain, scl-2, was isolated from isocarbophos-polluted soil. strain scl-2 was preliminarily identified as arthrobacter sp. based on its morphological, physiological, and biochemical proper ... | 2009 | 19996699 |
| complete degradation of di-n-octyl phthalate by biochemical cooperation between gordonia sp. strain jdc-2 and arthrobacter sp. strain jdc-32 isolated from activated sludge. | two bacterial strains were isolated from activated sludge using mixtures of phthalic acid esters (paes) as the sole source of carbon and energy. one of the isolates was identified as gordonia sp. strain jdc-2 and the other as arthrobacter sp. strain jdc-32, mainly through 16s rrna gene sequence analysis. gordonia sp. strain jdc-2 rapidly degraded di-n-octyl phthalate (dop) into phthalic acid (pa), which accumulated in the culture medium. arthrobacter sp. strain jdc-32 degraded pa but not dop. th ... | 2010 | 19959291 |
| [monosaccharide composition of exopolymer complex of bacteria-destructors of protective coatings]. | monosaccharide composition of exopolymer complex (epc) ofbacteria-destructors of protective coatings pseudomonas sp. t/, pseudomonas sp. 109, arthrobacter sp. 102 has been studied. it is established that monosaccharide composition of exopolymer complex of bacteria is different and depends on the bacteria growth model. it is mainly represented by penthoses and hexoses. glucose, galactose, arabinose, which are determined in epc of biofilm and plankton of all studied bacteria, are dominating monosa ... | 2009 | 19938612 |
| [isolation and identification of four dbp-degrading strains and molecular cloning of the degradation genes]. | four di-butyl-phthalate(dbp)-degrading bacterial strains, jdc-1, jdc-8, jdc-9 and jdc-12, were isolated from soil. the strains were gram positive. the 16s rrna sequence analysis revealed that the four strains had similarities of 99% with arthrobacter sp.. according to the morphologic, physiobiochemical characteristics and the analysis of their 16s rrna, all the four strains were identified as arthrobacter sp.. a 900 bp dna fragment was obtained from the four strains by pcr amplified and clone. w ... | 2009 | 19927831 |
| x-ray crystallographic analysis of the 6-aminohexanoate cyclic dimer hydrolase: catalytic mechanism and evolution of an enzyme responsible for nylon-6 byproduct degradation. | we performed x-ray crystallographic analyses of the 6-aminohexanoate cyclic dimer (acd) hydrolase (nyla) from arthrobacter sp., an enzyme responsible for the degradation of the nylon-6 industry byproduct. the fold adopted by the 472-amino acid polypeptide generated a compact mixed alpha/beta fold, typically found in the amidase signature superfamily; this fold was especially similar to the fold of glutamyl-trna(gln) amidotransferase subunit a (z score, 49.4) and malonamidase e2 (z score, 44.8). ... | 2010 | 19889645 |
| analysis of two gene clusters involved in the degradation of 4-fluorophenol by arthrobacter sp. strain if1. | arthrobacter sp. strain if1 is able to grow on 4-fluorophenol (4-fp) as a sole source of carbon and energy. to clone the 4-fp degradation genes, dna libraries were constructed and screened with a probe obtained by pcr using primers designed on the basis of conserved regions of aromatic two-component monooxygenases. sequencing of positive clones yielded two gene clusters, each harboring a gene encoding a monooxygenase with high sequence similarity to the oxygenase component of 4-nitrophenol and 4 ... | 2009 | 19837837 |
| soil bacterial communities in constructed wetlands treated with swine wastewater using pcr-dgge technique. | marsh-pond-marsh (mpm) constructed wetlands were designed for the treatment of swine wastewater. the goal of this study was to characterize bacterial communities in these wetlands and determine the nutrient removal from influent to effluent. surface soil samples were collected and analyzed by culture-dependent and culture-independent techniques. the results showed that the bacterial colony forming units (cfu) and the average concentrations of total nitrogen, nh(4)(+), total phosphorous (tp) and ... | 2010 | 19822421 |
| a new beta-galactosidase with a low temperature optimum isolated from the antarctic arthrobacter sp. 20b: gene cloning, purification and characterization. | a psychrotrophic bacterium producing a cold-adapted beta-galactosidase upon growth at low temperatures was classified as arthrobacter sp. 20b. a genomic dna library of strain 20b introduced into escherichia coli top10f' and screening on x-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside)-containing agar plates led to the isolation of beta-galactosidase gene. the beta-galactosidase gene (bgas) encoding a protein of 1,053 amino acids, with a calculated molecular mass of 113,695 kda. analys ... | 2009 | 19771412 |
| high-level chromate resistance in arthrobacter sp. strain fb24 requires previously uncharacterized accessory genes. | the genome of arthrobacter sp. strain fb24 contains a chromate resistance determinant (crd), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. the crd includes chra, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino and carboxy termini of chrb, a putative regulatory protein. there are also three novel genes that have not been previously associated with chromate resistance in other bacteria; they encode a ... | 2009 | 19758450 |
| fluorinated phenylcyclopropylamines. part 6: effects of electron withdrawing or donating aryl substituents on the inhibition of tyramine oxidase from arthrobacter sp. by diastereomeric 2-aryl-2-fluoro-cyclopropylamines. | diastereomeric arylcyclopropylamines substituted with fluorine in the 2-position and with electron donating or electron withdrawing groups at the aromatic ring were evaluated as inhibitors of microbial tyramine oxidase. the trans-isomers were consistently more potent inhibitors of the enzyme than the cis-isomers. electron donating substituents increased the potency of tyramine oxidase inhibition, while electron withdrawing substituents decreased the activity. the results obtained are discussed i ... | 2008 | 19727324 |
| genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition. | in this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for plant growth promoting (pgp) traits at higher salt (nacl) concentrations (2, 4, 6, and 8%). the results revealed that 24 rhizobacterial isolates were tolerant at 8% nacl. although all the 24 salt tolerable isolates produced indole-3-acetic acid (iaa), while 10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. however, only three isol ... | 2009 | 19701667 |
| atrazine and terbuthylazine mineralization by an arthrobacter sp. isolated from a sugarcane-cultivated soil in kenya. | a tropical soil from a kenyan sugarcane-cultivated field showed a very high capability to mineralize (14)c-ring-labeled atrazine. in laboratory experiments this soil mineralized about 90% of the applied atrazine within 98 d. the atrazine-degrading microbial community was enriched in liquid cultures containing atrazine as the sole n source and 100 mgl(-1) glucose as additional c source. from the enrichment culture a bacterial strain was isolated and identified by comparative sequence analysis of ... | 2009 | 19674769 |
| effects of bacterial treatment at early stages of atlantic cod (gadus morhua l.) on larval survival and development. | to assess the effects of bacterial treatment at the earliest stages of cod rearing on the microbial load, larval development and performance, testing three bacterial strains (carnobacterium divergens v41, arthrobacter sp. and enterococcus sp.) in vivo that were previously shown to have inhibitory potential towards fish pathogens in vitro. | 2010 | 19664064 |
| monitoring enzyme catalysis in the multimeric state: direct observation of arthrobacter 4-hydroxybenzoyl-coenzyme a thioesterase catalytic complexes using time-resolved electrospray ionization mass spectrometry. | the ability to examine real-time reaction kinetics for multimeric enzymes in their native state may offer unique insights into understanding the catalytic mechanism and its interplay with three-dimensional structure. in this study, we have used a time-resolved electrospray mass spectrometry approach to probe the kinetic mechanism of 4-hydroxybenzoyl-coenzyme a (4-hba-coa) thioesterase from arthrobacter sp. strain su in the millisecond time domain. intact tetrameric complexes of 4-hba-coa thioest ... | 2009 | 19635449 |
| a new cold-adapted beta-d-galactosidase from the antarctic arthrobacter sp. 32c - gene cloning, overexpression, purification and properties. | the development of a new cold-active beta-d-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. | 2009 | 19631003 |
| isolation and characterization of arthrobacter sp. hy2 capable of degrading a high concentration of p-nitrophenol. | a soil bacterium strain, capable of using p-nitrophenol (pnp) as its sole source of carbon and energy, was isolated by enrichment on minimal salt medium (msm). on the basis of a phylogenetic analysis of 16s rrna gene sequences the bacterium is a species of arthrobacter, closely related to arthrobacter ureafaciens dsm 20126. this strain has an unusually high substrate tolerance for pnp degradation in msm. greatest degradation of pnp was observed at 30 degrees c and under slightly alkaline ph (ph ... | 2009 | 19540107 |
| optimization of physical parameters for lipase production from arthrobacter sp. bgcc#490. | the physical parameters for the production of thermostable, alkaline lipase from arthrobacter sp. bgcc# 490 were optimized using response surface methodology (rsm), employing face centered central composite design (fcccd). the design was employed by selecting ph, temperature and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by rsm. a second-order quadratic model and response surface method showed that the optimum conditions for lipa ... | 2009 | 19517996 |
| batch and continuous biosorption of cu(2+) by immobilized biomass of arthrobacter sp. | the ability of free and polysulphone immobilized biomass of arthrobacter sp. to remove cu(2+) ions from aqueous solution was studied in batch and continuous systems. the langmuir and freundlich isotherm models were applied to the data. the langmuir isotherm model was found to fit the sorption data indicating that sorption was monolayer and uptake capacity (q(o)) was 175.87 and 158.7mg/g for free and immobilized biomass respectively at ph 5.0 and 30 degrees c temperature, which was also confirmed ... | 2009 | 19487070 |
| microbial responses to mustard gas dumped in the baltic sea. | microbiological studies were carried out on chemical weapon dump sites in the baltic sea. the effect of mustard gas hydrolysis products (mghps) on marine microbiota and the ability of microorganisms to degrade mghps were studied. many stations at the dump sites demonstrated reduced microbial diversity, and increased growth of species able to use mustard gas hydrolysis products as sole source of carbon. significant amounts of mghp-degrading bacteria were revealed in the near-bottom water. the mgh ... | 2009 | 19481794 |
| microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes. | in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ... | 2009 | 19477544 |
| [effect of a novel fibrinolytic enzyme fa-i on thrombosis and thrombolysis]. | to study the effect of fa-i, a novel fibrinolytic enzyme isolated from the metabolite of arthrobacter sp. dr-536, on thrombosis and thrombolysis in vivo. | 2009 | 19462909 |
| [effect of elevation of 1-hydroxy-2-naphthoic acid concentration on the lag phase of arthrobacter sp. k3: kinetic analysis]. | 2009 | 19449734 | |
| membrane bioreactor treatment of a simulated metalworking fluid wastewater containing ethylenediaminetetraacetic acid and dicyclohexylamine. | membrane bioreactors (mbrs) have been installed at automotive plants to treat metalworking fluid (mwf) wastewaters, which are known to contain toxic and/or recalcitrant organic compounds. a laboratory study was conducted to evaluate treatment of a simulated wastewater prepared from a semisynthetic mwf, which contains two such compounds, dicyclohexylamine (dcha) and ethylenediaminetetraacetic acid (edta). primary findings were as follows: during stable operating periods, almost all chemical oxyge ... | 2009 | 19445324 |
| improvement of catalytic properties of lipase from arthrobacter sp. by encapsulation in hydrophobic sol-gel materials. | in this work, lipase from arthrobacter sp. was immobilized by sol-gel encapsulation to improve its catalytic properties. various silanizing agents including vinyl-trimethoxy silane, octyl-trimethoxy silane, gamma-(methacryloxypropyl)-trimethoxy silane (maptms) and tetraethoxysilane (teos) were chosen as the precursors. among them, maptms was for the first time utilized to encapsulate lipases, and the prepared enzyme by copolymerization of maptms and teos exhibited the highest activity in both th ... | 2009 | 19428242 |
| high yield synthesis of 12-aminolauric acid by "enzymatic transcrystallization" of omega-laurolactam using omega-laurolactam hydrolase from acidovorax sp. t31. | the genes encoding omega-laurolactam hydrolases from cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, and sphingomonas sp. u238 were cloned and sequenced. nucleotide and amino acid sequence analysis of the four genes indicated that the primary structures of these omega-laurolactam hydrolases are significantly similar to the 6-aminohexanoate-cyclic-dimer hydrolase (ec 3.5.2.12). these genes were expressed in escherichia coli, and the omega-laurolactam hydrolysing activity of the reco ... | 2009 | 19420719 |
| effect of immobilization of a bacterial consortium on diuron dissipation and community dynamics. | this work intended to study the relationship between diuron herbicide dissipation and the population dynamics of co-cultivated delftia acidovorans wdl34 (wdl34) and arthrobacter sp. n4 (n4) for different cell formulations: free cells or immobilization in ca-alginate beads of one or both strains. gfp-tagged wdl34 and n4 gram staining allowed analyzing the cell growth and distribution of each strain in both beads and culture medium in the course of the time. compared to the free cell co-culture of ... | 2009 | 19386487 |
| high rate of n2 fixation by east siberian cryophilic soil bacteria as determined by measuring acetylene reduction in nitrogen-poor medium solidified with gellan gum. | for evaluating n(2) fixation of diazotrophic bacteria, nitrogen-poor liquid media supplemented with at least 0.5% sugar and 0.2% agar are widely used for acetylene reduction assays. in such a soft gel medium, however, many n(2)-fixing soil bacteria generally show only trace acetylene reduction activity. here, we report that use of a n(2) fixation medium solidified with gellan gum instead of agar promoted growth of some gellan-preferring soil bacteria. in a soft gel medium solidified with 0.3% ge ... | 2009 | 19286791 |
| industrial production of difructose anhydride iii (dfa iii) from crude inulin extracted from chicory roots using arthrobacter sp. h65-7 fructosyltransferase. | a practical, economical, and industrial process for the enzymatic production of difructose anhydride iii (dfa iii) was investigated for crude inulin prepared from chicory roots using arthrobacter sp. h65-7 fructosyltransferase. a comparable level of dfa iii production to that from commercial inulin was obtained using crude inulin, suggesting the feasibility of this production process. | 2009 | 19269589 |
| global proteomic analysis of the chromate response in arthrobacter sp. strain fb24. | a global proteomic evaluation of the response of arthrobacter sp. strain fb24 to 5 and 20 mm cr(vi) was conducted using both two-dimensional gel electrophoresis (2-dge) and liquid chromatography coupled with tandem mass spectrometry (lc/lc-ms/ms). the changes in protein expression found with 2-dge indicate alterations in central metabolism and amino acid synthesis. proteome coverage increased from 22% with 2-dge to 71% with lc/lc-ms/ms. the proteins exhibiting the highest levels of expression un ... | 2009 | 19231868 |
| purification and characterization of arsenite oxidase from arthrobacter sp. | the chemolithoautotroph, arthrobacter sp.15b oxidizes arsenite to arsenate using a membrane bound arsenite oxidase. the enzyme arsenite oxidase is purified to its homogeneity and identified using maldi-tof ms analysis. upon further characterization, it was observed that the enzyme is a heterodimer showing native molecular mass as approximately 100 kda and appeared as two subunits of approximately 85 kda lsu and 14 kda ssu on sds-page. the v(max) and k(m) values of the enzyme was found to be 2.45 ... | 2009 | 19214757 |
| arthrobacter phenanthrenivorans sp. nov., to accommodate the phenanthrene-degrading bacterium arthrobacter sp. strain sphe3. | a novel phenanthrene-degrading bacterium, designated strain sphe3(t), was isolated from a creosote-contaminated soil in greece. cells were non-motile, gram-positive, aerobic, and rod- to coccus-shaped. the strain was isolated on the basis of formation of a clear zone on agar plates sprayed with phenanthrene. optimal growth occurred at 30 degrees c. the g+c content of the dna was 65.7 mol%. the polar lipid pattern of strain sphe3(t) consisted of phosphatidylethanolamine, phosphatidylglycerol and ... | 2009 | 19196765 |
| preferential hydrolysis of aberrant intermediates by the type ii thioesterase in escherichia coli nonribosomal enterobactin synthesis: substrate specificities and mutagenic studies on the active-site residues. | the type ii thioesterase enth is a hotdog fold protein required for optimal nonribosomal biosynthesis of enterobactin in escherichia coli. its proposed proofreading activity in the biosynthesis is confirmed by its efficient restoration of enterobactin synthesis blocked in vitro by analogs of the cognate precursor 2,3-dihydroxybenzoate. steady-state kinetic studies show that enth recognizes the phosphopantetheine group and the pattern of hydroxylation in the aryl moiety of its thioester substrate ... | 2009 | 19193103 |
| biodegradation of mixed phenolic compounds under high salt conditions and salinity fluctuations by arthrobacter sp. w1. | high salt concentration and salinity fluctuations in wastewater challenge the efficiency of microbial strains used for cleanup of pollutants. in this study, it was investigated that the new isolated arthrobacter sp. w1 degraded mixed phenolic compounds under complex salt conditions. the results showed that arthrobacter sp. w1 was able to utilize various phenolic compounds as carbon source under high salt conditions. it can degrade phenol and p-cresol mixture at 10% nacl, although rates of degrad ... | 2009 | 19156367 |
| isolation and characterization of atrazine-degrading arthrobacter sp. ad26 and use of this strain in bioremediation of contaminated soil. | a bacterial strain (ad26) capable of utilizing atrazine as a sole nitrogen source for growth was isolated from an industrial wastewater sample by enrichment culture. the 16s rrna gene sequencing identified ad26 as an arthrobacter sp. pcr assays indicated that ad26 contained atrazine-degrading genes trzn and atzbc. the trzn gene of ad26 only differs from the trzn of arthrobacter aurescens tc1 by one base (a-->t at 907) and one amino acid (met-->leu at 303). the specific activity of trzn of ad26 i ... | 2008 | 19143347 |
| isolation and characterization of mesotrione-degrading bacillus sp. from soil. | dissipation kinetics of mesotrione, a new triketone herbicide, sprayed on soil from limagne (puy-de-dôme, france) showed that the soil microflora were able to biotransform it. bacteria from this soil were cultured in mineral salt solution supplemented with mesotrione as sole source of carbon for the isolation of mesotrione-degrading bacteria. the bacterial community structure of the enrichment cultures was analyzed by temporal temperature gradient gel electrophoresis (ttge). the ttge fingerprint ... | 2009 | 19121884 |
| arthrobacter sp. lipase immobilization for preparation of enantiopure masked beta-amino alcohols. | recent reports on immobilization of lipase from arthrobacter sp. (abl, mtcc 5125; iiim isolate) on insoluble polymers have shown altered properties including stability and enantioselectivity. present work demonstrates a facile method for the preparation of enantiopure beta-amino alcohols by modulation of abl enzyme properties via immobilization on insoluble as well as soluble supports using entrapment/covalent binding techniques. efficacies of immobilized abl on insoluble supports prepared from ... | 2009 | 19081255 |
| metabolic cooperation of gordonia sp. strain mtcc 4818 and arthrobacter sp. strain wy in the utilization of butyl benzyl phthalate: effect of a novel co-culture in the degradation of a mixture of phthalates. | degradation of butyl benzyl phthalate (bbp) by a co-culture of gordonia sp. strain mtcc 4818 and arthrobacter sp. strain wy was investigated. in the degradation of bbp by the co-culture, the limitations of the individual species in metabolizing bbp were overcome, leading to the development of a consortium capable of complete utilization of this ester. in the degradation of bbp by the co-culture, the presence of multiple esterases was demonstrated in both species by activity staining of non-denat ... | 2008 | 18957587 |
| a novel aryl acylamidase from nocardia farcinica hydrolyses polyamide. | an alkali stable polyamidase was isolated from a new strain of nocardia farcinica. the enzyme consists of four subunits with a total molecular weight of 190 kda. the polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hydrolyzed different soluble amides to the corresponding acid. treatment of polyamide 6 with this amidase led to an increased hydrophilicity based on rising height and tensiometry mea ... | 2009 | 18942140 |
| mineralization of p-nitrophenol by a new isolate arthrobacter sp. y1. | arthrobacter sp. y1, capable of metabolizing p-nitrophenol (pnp) as the sole carbon, nitrogen and energy source was isolated from activated sludge. the bacterium could tolerate concentrations of pnp up to 600 mg l(- 1), and degradation of pnp was achieved within 120 h of incubation. pnp and its metabolites were analyzed by high performance liquid chromatography (hplc). the metabolite formed indicated that the organism followed the 4-nitrocathechol (4-nc) pathway for metabolism of this compound. ... | 2008 | 18941993 |
| plasmid as a measure of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid. | the purpose of this research was to pursuit the quantification of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid (2,4-d) by detecting and quantifying a prominent 2,4-d degradation encoding plasmid. batch reactor acclimation, de-acclimation, and re-acclimation tests were conducted during which periods the courses of 2,4-d dissipation and plasmid evolution were quantitatively measured. pure cultures of bacterial strains were detected to give rise to a plasmid approximately the s ... | 2009 | 18930390 |
| enzymatic characterization of an amine oxidase from arthrobacter sp. used to measure phosphatidylethanolamine. | ethanolamine oxidase was screened with the aim of using it to establish a novel enzymatic phosphatidylethanolamine assay. ethanolamine oxidase activity was detected in the crude extract of arthrobacter sp., and the enzyme was purified more than 15-fold in three steps with a 54% yield. sds-page revealed the presence of only one band, which migrated, with an apparent molecular mass of 70 kda. biochemical characterization of the enzyme showed phenylethylamine to be the preferred substrate, with the ... | 2008 | 18838796 |
| application of 16s rdna-dgge and plate culture to characterization of bacterial communities associated with the sawfly, acantholyda erythrocephala (hymenoptera, pamphiliidae). | culture-based analysis was employed in parallel with pcr amplification of 16s rdna, coupled with denaturing gradient gel electrophoresis (dgge), to profile bacterial species associated with different developmental stages of the pine false webworm (pfw), acantholyda erythrocephala, a sawfly pest responsible for incidents of severe defoliation in commercially important tree plantations in north america. culture-based analysis revealed that pseudomonas spp. along with bacillus sphaericus and arthro ... | 2008 | 18769850 |
| genome sequence of the fish pathogen renibacterium salmoninarum suggests reductive evolution away from an environmental arthrobacter ancestor. | renibacterium salmoninarum is the causative agent of bacterial kidney disease and a significant threat to healthy and sustainable production of salmonid fish worldwide. this pathogen is difficult to culture in vitro, genetic manipulation is challenging, and current therapies and preventative strategies are only marginally effective in preventing disease. the complete genome of r. salmoninarum atcc 33209 was sequenced and shown to be a 3,155,250-bp circular chromosome that is predicted to contain ... | 2008 | 18723615 |
| the screening, characterization, and use of omega-laurolactam hydrolase: a new enzymatic synthesis of 12-aminolauric acid. | several omega-laurolactam degrading microorganisms were isolated from soil samples. these strains were capable of growing in a medium containing omega-laurolactam as sole source of carbon and nitrogen. among them, five strains (t7, t31, u124, u224, and u238) were identified as cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, rhodococcus sp. u224, and sphingomonas sp. u238, respectively. the omega-laurolactam hydrolyzing enzyme from rhodococcus sp. u224 was purified to homogeneity, a ... | 2008 | 18685217 |
| toxicity of homologous series of organic solvents for the gram-positive bacteria arthrobacter and nocardia sp. and the gram-negative bacteria acinetobacter and pseudomonas sp. | the toxicity of homologous series of organic solvents has been investigated for the gram-positive bacteria, arthrobacter sp. and nocardia sp., and the gram-negative bacteria, acinetobacter sp. and pseudomonas sp. the hydrophobicity of the solvent, expressed by its logp(octanol), proves to be a good measure for the toxicity of solvents in a two-phase system. the transition from toxic to nontoxic solvents occurs between logp(octanol) 3 and 5 and depends on the homologous series. no correlation has ... | 1993 | 18613108 |
| multi-compound electrophoretic assays for tyramine oxidase with a uv area detector imaging multiple windows on a looped capillary. | active pixel sensor uv area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (emma) assay for substrate specificity of tyramine oxidase (arthrobacter sp.). use of the uv area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. continuo ... | 2008 | 18589431 |
| selection and identification of bacterial strains with methyl-tert-butyl ether, ethyl-tert-butyl ether, and tert-amyl methyl ether degrading capacities. | nine bacterial strains isolated from two hydrocarbon-contaminated soils were selected because of their capacity for growth in culture media amended with 200 mg/l of one of the following gasoline oxygenates: methyl-tert-butyl ether (mtbe), ethyl-tert-butyl ether (etbe), and tert-amyl methyl ether (tame). these strains were identified by amplification of their 16s rrna gene, using fdl and rd1 primers, and were tested for their capacity to grow and biotransform these oxygenates in both mineral and ... | 2008 | 18522454 |
| [restriction endonuclease asi256i recognizing the nucleotide sequence 5'-gatc-3']. | a strain producing a restriction endonuclease was isolated from soil samples and identified as the arthrobacter sp. strain ck256. the enzyme produced by this strain was termed asi2561. the isolation procedure for this enzyme was described, and the optimal conditions for its function were determined. it was shown that the restriction endonuclease asi256i is a true isoschizomer of mboi, it has a temperature optimum of 6 degrees c, and can be used in molecular-biological and genetic-engineering stu ... | 2008 | 18491594 |