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dynamics of microbial community during ensiling direct-cut alfalfa with and without lab inoculant and sugar.to gain deeper insights into the clostridial community dynamics and chemical transformations during the ensiling of alfalfa.201728370869
[weissella confusa bacteremia: an underestimated opportunistic pathogen]. 201728325577
draft genome sequence of weissella confusa mbf8-1, a glucansucrase- and bacteriocin-producing strain isolated from a homemade soy product.we report here the draft genome sequence of weissella confusa mbf8-1, an isolate from a homemade fermented soybean product that produces sucrases and exhibits antibacterial (bacteriocin) activity. the draft genome of w. confusa mbf8-1 comprises a 2.2-mbp chromosome and a 17.8-kbp bacteriocin-encoding plasmid. two putative glucansucrase genes were also identified.201728126938
natural lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant.natural lactic acid bacteria (lab) populations in tropical grasses and their fermentation characteristics on silage prepared with cellulase enzyme and lab inoculants were studied. a commercial inoculant lactobacillus plantarum chikuso 1 (ch), a local selected strain lactobacillus casei th14 (th14), and 2 cellulases, acremonium cellulase (ac) and maicelase (mc; meiji seika pharma co. ltd., tokyo, japan), were used as additives to silage preparation with fresh and wilted (6 h) guinea grass and nap ...201627743669
physico-chemical characterization of galactan exopolysaccharide produced by weissella confusa kr780676.physico-chemical properties of a galactan exopolysaccharide (eps) produced by weissella confusa kr780676 isolated from an idli batter were studied. in our previous paper, we had reported the isolation and characterization of a linear galactan (containing α-(1→6)-linked galactose units) isolated from an acidic fermented food. in this manuscript, we are reporting the physico-chemical properties viz., colour evaluation (l* 81.79, a* 3.15, b* 15.04), flow properties (compressibility index 38.46%), s ...201627645923
intestinal lactobacillus community structure and its correlation with diet of southern chinese elderly subjects.this study aimed to investigate the relationship between the intestinal lactobacillus species and diet of elderly subjects in a longevity area in southern china. healthy elderly subjects ranging from 80 to 99 years old were respectively selected from the regions of bama and nanning, guangxi, china. the nested polymerase chain reaction and denaturing gradient gel electrophoresis (pcr-dgge) technology was used to analyze the intestinal lactobacillus community structure. results showed that weissel ...201627572508
molecular cloning of kman coding for mannanase from klebsiella oxytoca kub-cw2-3 and its hybrid mannanase characters.gene encoding for β-mannanase (e.c 3.2.1.78) from klebsiella oxytoca kub-cw2-3 was cloned and expressed by an e. coli system resulting in 400 times higher mannanase activities than the wild type. a 3314bp dna fragment obtained revealed an open reading frame of 1164bp, namely kman-2, which encoded for 387 amino acids with an estimated molecular weight of 43.2kda. it belonged to the glycosyl hydrolase family 26 (gh26) exhibited low similarity of 50-71% to β-mannanase produced by other microbial so ...201627233126
probiotic properties of lactic acid bacteria isolated from human milk.the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties.201627159339
community dynamics and metabolite target analysis of spontaneous, backslopped barley sourdough fermentations under laboratory and bakery conditions.barley flour is not commonly used for baking because of its negative effects on bread dough rheology and loaf volume. however, barley sourdoughs are promising ingredients to produce improved barley-based breads. spontaneous barley sourdough fermentations were performed through backslopping (every 24h, 10days) under laboratory (fermentors, controlled temperature of 30°c, high dough yield of 400) and bakery conditions (open vessels, ambient temperature of 17-22°c, low dough yield of 200), making u ...201627088869
optimization of isomaltooligosaccharide size distribution by acceptor reaction of weissella confusa dextransucrase and characterization of novel α-(1→2)-branched isomaltooligosaccharides.long-chain isomaltooligosaccharides (imos) are promising prebiotics. imos were produced by a weissella confusa dextransucrase via maltose acceptor reaction. the inputs of substrates (i.e., sucrose and maltose, 0.15-1 m) and dextransucrase (1-10 u/g sucrose) were used to control imo yield and profile. according to response surface modeling, 1 m sucrose and 0.5 m maltose were optimal for the synthesis of longer imos, whereas the dextransucrase dosage showed no significant effect. in addition to th ...201627050481
identification and sequence analysis of pwcmbf8-1, a bacteriocin-encoding plasmid from the lactic acid bacterium weissella confusa.members of the gram-positive lactic acid bacteria (lab) are well-known for their beneficial properties as starter cultures and probiotics. many lab species produce ribosomally synthesized proteinaceous antibiotics (bacteriocins). weissella confusa mbf8-1 is a strain isolated from a fermented soybean product that not only produces useful exopolysaccharides but also exhibits bacteriocin activity, which we call weissellicin mbf. here, we show that bacteriocin production by w. confusa mbf8-1 is spec ...201626976853
antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens.the addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives.201526957978
microbial shifts associated with necrotic enteritis.an outbreak of necrotic enteritis (ne) is a complex process requiring one or a number of predisposing factors rather than just the presence of pathogenic clostridium perfringens. examples are dietary influences, such as high levels of non-starch polysaccharides and fishmeal, and factors that evoke epithelial cell damage, such as fusarium mycotoxins in feed and eimeria infections. recent studies have shown that different predisposing factors induce similar shifts in the intestinal microbiota comp ...201626950294
structure modeling and functional analysis of recombinant dextransucrase from weissella confusa cab3 expressed in lactococcus lactis.the dextransucrase gene from weissella confusa cab3, having an open reading frame of 4.2 kb coding for 1,402 amino acids, was amplified, cloned, and expressed in lactococcus lactis. the recombinant dextransucrase, wccab3-rdsr was expressed as extracellular enzyme in m17 medium with a specific activity of 1.5 u/mg which after purification by peg-400 fractionation gave 6.1 u/mg resulting in 4-fold purification. wccab3-rdsr was expressed as soluble and homogeneous protein of molecular mass, approxi ...201626861959
ethanol production by selected intestinal microorganisms and lactic acid bacteria growing under different nutritional conditions.to gain some specific insight into the roles microorganisms might play in non-alcoholic fatty liver disease (nafld), some intestinal and lactic acid bacteria and one yeast (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, enterococcus fecalis, escherichia coli, lactobacillus acidophilus, lactobacillus fermentum, lactobacillus plantarum, weissella confusa, saccharomyces cerevisiae) were characterized by high performance liquid chromatography for production of ethanol whe ...201626858714
characterization of a novel galactan produced by weissella confusa kr780676 from an acidic fermented food.an exopolysaccharide (eps) producing strain pufstm055 isolated from idli batter (an indian traditional cereal-legume based fermented food) was identified as weissella confusa kr780676. the strain was shown to produce 17.2g/l (dry weight) of eps in 2% sucrose supplemented mrs broth and the eps was characterized. hptlc analysis confirmed the presence of galactose monomers, indicating the homopolysaccharide nature of eps. fourier-transform infrared spectroscopy and nuclear magnetic resonance analys ...201626836614
quantification of biofilm exopolysaccharides using an in situ assay with periodic acid-schiff reagent.a novel approach to the quantification of extracellular polysaccharides in miniaturized biofilms presenting a wide variety of extracellular matrices was developed. the assay used the periodic acid-schiff reagent and was first calibrated on dextran and alginate solutions. then it was implemented on 24-h and 48-h biofilms from three strains known to produce different exopolymeric substances (pseudomonas aeruginosa, bacillus licheniformis, weissella confusa). the assay allowed quantification of the ...201626800864
lactic acid bacteria in dried vegetables and spices.spices and dried vegetable seasonings are potential sources of bacterial contamination for foods. however, little is known about lactic acid bacteria (lab) in spices and dried vegetables, even though certain lab may cause food spoilage. in this study, we enumerated lab in 104 spices and dried vegetables products aimed for the food manufacturing industry. the products were obtained from a spice wholesaler operating in finland, and were sampled during a one-year period. we picked isolates (n = 343 ...201626678137
rye bran as fermentation matrix boosts in situ dextran production by weissella confusa compared to wheat bran.the consumption of fiber-rich foods such as cereal bran is highly recommended due to its beneficial health effects. pre-fermentation of bran with lactic acid bacteria can be used to improve the otherwise impaired flavor and textural qualities of bran-rich products. these positive effects are attributed to enzymatic modification of bran components and the production of functional metabolites like organic acids and exopolysaccharides such as dextrans. the aim of this study was to investigate dextr ...201626649737
screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil.lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ...201626608755
identification of weissella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.although some weissella species play beneficial roles in food fermentation and in probiotic products, others such as weissella confusa are emerging gram-positive pathogens in immunocompromised hosts. weissella species are difficult to identify by conventional biochemical methods and commercial automated systems and are easily misidentified as lactobacillus and leuconostoc species. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly being us ...201526594208
purification and characterization of bacteriocin produced by weissella confusa a3 of dairy origin.a dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. therefore, antimicrobial peptides (amps) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. the present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. the bacteriocin was isolated from weissella confusa a3 that was isolated fr ...201526474074
exopolysaccharides from co-cultures of weissella confusa 11gu-1 and propionibacterium freudenreichii js15 act synergistically on wheat dough and bread texture.the storage of bread is limited by both physical (staling) and microbial (mainly fungal) spoilage. exopolysaccharides (eps) produced by lactic acid bacteria (lab) and organic acids from propionibacteria (pab) have been used to enhance texture and extend shelf-life of bakery products. in this study the functionality of eps of weissella confusa a3/2-1 (dextran), w. confusa f3/2-2 (dextran and levan), w. confusa 11gu-1 (dextran and ropy capsular polysaccharide) was evaluated in wheat bread. two str ...201526256717
lactose- and cellobiose-derived branched trisaccharides and a sucrose-containing trisaccharide produced by acceptor reactions of weissella confusa dextransucrase.dextran-producing weissella have received significant attention. however, except for maltose, the acceptor reactions of weissella dextransucrases with different sugars have not been investigated. the action of recombinant weissella confusa vtt e-90392 dextransucrase was tested with several potential acceptors, particularly, analogs lactose and cellobiose. the major acceptor products of both disaccharides were identified as branched trisaccharides, with a glucosyl residue α-(1 → 2)-linked to the ...201626212965
distinctive intestinal lactobacillus communities in 6-month-old infants from rural malawi and southwestern finland.our aim was to compare the composition and diversity of lactobacillus microbiota in infants living in malawi and southwestern finland.201526049782
the impact of fermentation with exopolysaccharide producing lactic acid bacteria on rheological, chemical and sensory properties of pureed carrots (daucus carota l.).fermentation with lactic acid bacteria (lab) offers a natural means to modify technological and nutritional properties of foods and food ingredients. this study explored the impact of fermentation with different exopolysaccharide (eps) producing lab on rheological, chemical and sensory properties of puréed carrots in water, as a vegetable model, with the focus on texture formation. the screening of 37 lab strains for starter selection revealed 16 lactobacillus, leuconostoc and weissella strains ...201526001525
reply to "confused by weissella confusa bacteremia". 201525976900
confused by weissella confusa bacteremia. 201525976899
epicocconone, a sensitive and specific fluorescent dye for in situ quantification of extracellular proteins within bacterial biofilms.biofilms are ecosystems of closely associated bacteria encapsulated in an extracellular matrix mainly composed of polysaccharides and proteins. a novel approach was developed for in situ quantification of extracellular proteins (epns) in various bacterial biofilms using epicocconone, a natural, fluorescent compound that binds amine residues of proteins. six commercial proteins were tested for their reaction with epicocconone, and bovine serum albumin (bsa) was selected for assay optimization. th ...201525913004
construction of a shuttle vector based on the small cryptic plasmid pjy33 from weissella cibaria 33.a cryptic plasmid, pjy33, from weissella cibaria 33 was characterized. pjy33 was 2365 bp in size with a gc content of 41.27% and contained two putative open reading frames (orfs). orf1 encoded a putative hypothetical protein of 134 amino acids. orf2 was 849 bp in size, and its putative translation product exhibited 87% identity with a replication initiation factor from a plasmid from w. cibaria klc140. a weissella-escherichia coli shuttle vector, pjy33e (6.5 kb, em(r)), was constructed by ligati ...201525882072
in vitro modulation of tumor necrosis factor α production in thp-1 cells by lactic acid bacteria isolated from healthy human infants.the human microbiota is a source of probiotics capable of modulating the host immune system. in this study, we collected fecal samples from 100 healthy infants and isolated lactic acid bacteria which were screened for immune modulating effects on tumor necrosis factor α (tnf-α) production. cell-free culture supernatants from 26 isolates were able to decrease tnf-α production in vitro and three of the isolates were selected as candidate probiotics (msmc39-1, msmc39-3, msmc57-1). these isolates we ...201525759008
photo quiz: confusing bacteremia in a crohn's disease patient. weissella confusa. 201525700525
genomics of weissella cibaria with an examination of its metabolic traits.weissella is a genus of lactic acid bacteria (lab) consisting of species formerly included in the leuconostoc paramesenteroides group. similar to other lab, they are commonly found in fermented foods but have also been isolated from environmental and human samples. currently there are 20 recognized species. herein, three weissella cibaria genomes were sequenced using illumia mi-seq and roche 454 technologies. annotation was performed using the prokka and jgi img pipelines. a thorough analysis of ...201525678547
cloning and characterization of a weissella confusa dextransucrase and its application in high fibre baking.wheat bran offers health benefits as a baking ingredient, but is detrimental to bread textural quality. dextran production by microbial fermentation improves sourdough bread volume and freshness, but extensive acid production during fermentation may negate this effect. enzymatic production of dextran in wheat bran was tested to determine if dextran-containing bran could be used in baking without disrupting bread texture. the weissella confusa vtt e-90392 dextransucrase gene was sequenced and his ...201525603169
pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage from northwestern argentina.the diversity of lactic acid bacteria (lab) associated with chicha, a traditional maize-based fermented alcoholic beverage from northwestern argentina, was analyzed using culture-dependent and culture-independent approaches. samples corresponding to 10 production steps were obtained from two local producers at maimará (chicha m) and tumbaya (chicha t). whereas by culture-dependent approach a few number of species (lactobacillus plantarum and weissella viridescens in chicha m, and enterococcus fa ...201525584777
iranian wheat flours from rural and industrial mills: exploitation of the chemical and technology features, and selection of autochthonous sourdough starters for making breads.this study aimed at describing the main chemical and technology features of eight iranian wheat flours collected from industrial and artisanal mills. their suitability for bread making was investigated using autochthonous sourdough starters. chemical analyses showed high concentration of fibers and ash, and technology aptitude for making breads. as shown through 2-de analyses, gliadin and glutenin subunits were abundant and varied among the flours. according to the back slopping procedure, type ...201525583343
characterization of exopolysaccharide and ropy capsular polysaccharide formation by weissella.with their broad functional properties, lactic acid bacteria derived high molar mass exopolysaccharides (eps) and oligosaccharides are of great interest for food, medical and pharmaceutical industry. eps formation by 123 strains of weissella cibaria and weissella confusa, was evaluated. dextran formation from sucrose was observed for all tested strains while 18 strains produced fructan in addition to dextran. six isolates synthesized a highly ropy polymer from glucose associated with the formati ...201525475311
cloning and heterologous expression of the ftfcnc-2(1) gene from weissella confusa mbfcnc-2(1) as an extracellular active fructansucrase in bacillus subtilis.fructan-exopolysaccharides (fructan-eps) (inulin and levan) and their oligosaccharides (fructooligosaccharides, fos) have drawn considerable interest in the food and pharmaceutical industries. eps-producing lactic acid bacteria have been reported to produce β-fructans (inulin and levan), as well as α-glucans, by the function of sucrase enzymes, i.e., fructansucrase and glucansucrase. a fructansucrase ftfcnc-2(1) gene from weissella confusa strain mbfcnc-2(1) was previously cloned in escherichia ...201525454699
unpasteurised commercial boza as a source of microbial diversity.boza is a cereal-based fermented beverage widely consumed in many countries of the balkans. the aim of this study was to investigate the microbiota of three bulgarian boza samples through a combination of culture-dependent and -independent methods with the long-term objective of formulating a multi-strain starter culture specifically destined for the manufacture of new cereal-based drinks. the isolation campaign for lactic acid bacteria (lab) allowed the identification of lactobacillus parabuchn ...201525437059
a confusing case - weissella confusa prosthetic joint infection: a case report and review of the literature.the authors describe the first case of weissella confusa infection of a prosthetic joint. identification of the pathogen required 16s ribosomal rna sequencing of isolates obtained on two separate occasions during the assessment of an elderly woman with a painful, swollen knee following total knee arthroplasty. a review of reported human infections due to w confusa are summarized, and risk factors and pitfalls in the application of empirical antimicrobial therapy pending definitive microbiologica ...201425285116
selection of functional lactic acid bacteria as starter cultures for the fermentation of korean leek (allium tuberosum rottler ex sprengel.).the purpose of this research was to find safe and suitable starter cultures for the fermentation of korean leek (allium tuberosum rottler), also known as garlic chives or oriental garlic. this traditional herb has several functional properties and a strong flavour; its leaves are used as food material. eighteen strains of lactic acid bacteria (lab) were isolated from well-fermented leek kimchi. controlled fermentation of the leek leaves was conducted with 2 strains (weissella confusa lk4 and lac ...201425279760
modeling and investigation of submerged fermentation process to produce extracellular polysaccharide using lactobacillus confusus.the main objective of the present study is to investigate and optimize the submerged fermentation (smf) process parameters such as addition of coconut water, nacl dose, incubation time and temperature on the production of extracellular polysaccharide (eps) and biomass production using lactobacillus confuses. response surface methodology (rsm) coupled with four factors three level box-behnken design (bbd) was employed to model the smf process. rsm analysis indicated good correspondence between ex ...201425263862
effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content.the effects of storage period and aerobic deterioration on the bacterial community were examined in italian ryegrass (ir), guinea grass (gg), and whole-crop maize (wm) silages. direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives; live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-butanediol, acetic acid, and lactic acid were the dominant fermentation products in the ir, gg, and w ...201325049913
cytotoxicity comparison of quercetin and its metabolites from in vitro fermentation of several gut bacteria.part of quercetin is coerced into the colon after ingestion and interacts with the gut microbiota. the interaction between quercetin and gut microbiota will influence human health. the cytotoxicity of quercetin and its metabolites from human gut bacteria in vitro fermentation was investigated in this study. the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) (mtt) and agar diffusion disc methods were individually applied in vitro to examine their inhibitory effect on three cultured ...201425015032
weissella confusa: problems with identification of an opportunistic pathogen that has been found in fermented foods and proposed as a probiotic.weissella confusa is found in fermented foods and has been suggested as a probiotic, but also causes sepsis and other serious infections in humans and animals. the incidence of human infections is underestimated partly due to confusion with viridans streptococci and partly due to difficulty making a definitive identification, even if the organism is recognized to belong to another genus, owing to the inability of commercial organism systems to identify it. we report our experiences identifying w ...201424971076
exopolysaccharides from lactic acid bacteria: structural analysis, molecular weight effect on immunomodulation.exopolysaccharides (eps) obtained from the culture medium of lactobacillus confusus tistr 1498 were investigated to determine their molecular characteristics and the effect of molecular weight (mw) on immunomodulatory activity. the eps mainly consisted of carbohydrates (81.9±2.4%) with only one type of monosaccharide, d-glucose, which was mostly connected by α-(1→6) glycosidic linkages. the eps itself was unable to stimulate raw264.7 cells to produce pro-inflammatory mediator nitric oxide (no) a ...201424820155
isolation and identification of quercetin degrading bacteria from human fecal microbes.quercetin has a wide range of biological properties. the gut microflora can often modulate its biological activity and their potential health effects. there still is a lack of information about gut bacteria involving in this process. the strains of gut microbes from human feces that can transform quercetin were isolated and identified by in vitro fermentation. the results showed that escherichia coli, stretococcus lutetiensis, lactobacillus acidophilus, weissella confusa, enterococcus gilvus, cl ...201424594786
microbiology of pozol, a mexican fermented maize dough.mexican fermented maize dough, pozol, including traditional banana leaf-wrapped samples and material in plastic bags, was purchased. all samples were ph 4.7 to 5.7 approx. 12 h after preparation, ph declining to 3.6 to 3.9 after 6 to 9 days storage at ambient temperature. these latter samples had dry matter contents of 31% to 48% (w/w), 0.35% to 0.75% titratable acidity as lactic acid and lactic acid bacteria as predominant microbial flora at about 10(8) c.f.u./ml. the lactic acid bacteria inclu ...199524414915
weissella confusa septicemia in a foal.weissella confusa is a gram-positive bacterium that has been identified in environmental and food samples from around the world. rare cases of bacteremia in immunocompromised people have been reported. a 2-day-old foal was presented for weakness and suspected sepsis. blood culture yielded pure growth of a gram-positive coccobacillus, which was identified as w. confusa through sequencing of the 16s ribosomal dna. although the foal initially responded to antimicrobial therapy with ceftiofur and me ...201424327736
weissella confusa cab3 dextransucrase: properties and in vitro synthesis of dextran and glucooligosaccharides.food-derived weissella spp. have gained attention during recent years as efficient dextran producers. weissella confusa cab3 dextransucrase (wccab3-dsr) was isolated applying peg fractionation and used for in vitro synthesis of dextran and glucooligosaccharides. wccab3-dsr had a molar mass of 178 kda and was activated by co(2+) and ca(2+) ions. glycerol and tween 80 enhanced enzyme stability, and its half-life at 30°c increased from 10h to 74 h and 59 h, respectively. the (1)h and (13)c nmr spec ...201424299811
challenges in analysis of high-molar mass dextrans: comparison of hpsec, asflfff and dosy nmr spectroscopy.dilute solutions of various dextran standards, a high-molar mass (hmm) commercial dextran from leuconostoc spp., and hmm dextrans isolated from weissella confusa and leuconostoc citreum were analyzed with high-performance size-exclusion chromatography (hpsec), asymmetric flow field-flow fractionation (asflfff), and diffusion-ordered nmr spectroscopy (dosy). hpsec analyses were performed in aqueous and dimethyl sulfoxide (dmso) solutions, while only aqueous solutions were utilized in asflfff and ...201424274497
weissella confusa bacteremia in an immune-competent patient with underlying intramural hematomas of the aorta. 201324205498
identification of lactic acid bacteria associated with traditional cachaça fermentations.during the production of traditional cachaça (alembic´s cachaça), contamination of the fermented must is one of the factors leading to economic losses in the beverage manufacturing industry. the diversity of bacterial populations and the role of these microorganisms during the cachaça production process are still poorly understood in brazil. in our work, the fermentation process was followed in two distilleries located in the state of minas gerais. the objective of this work was to identify the ...201024031520
antibacterial activities of multi drug resistant myroides odoratimimus bacteria isolated from adult flesh flies (diptera: sarcophagidae) are independent of metallo beta-lactamase gene.sarcophagidae) are well known cause of myiasis and their gut bacteria have never been studied for antimicrobial activity against bacteria. antimicrobial studies of myroides spp. are restricted to nosocomial strains. a gram-negative bacterium, myroides sp., was isolated from the gut of adult flesh flies (sarcophaga sp.) and submitted to evaluation of nutritional parameters using biolog gn, 16s rrna gene sequencing, susceptibility to various antimicrobials by disc diffusion method and detection of ...200824031236
stress response of some lactic acid bacteria isolated from romanian artisan dairy products.understanding the mechanisms of stress response and adaptation to stress in the case of lactic acid bacteria (lab), especially in the case of strains with functional properties, is very important when such strains are potential candidates for starter cultures or probiotics. in this context, our study shows the response of some lab [four exopolysaccharide (eps)-producing strains and one strain with potential probiotic effect] to the stresses induced by low and high incubation temperatures, acidit ...201423933743
evidence for xylooligosaccharide utilization in weissella strains isolated from indian fermented foods and vegetables.six strains isolated from fermented food were identified as weissella species by 16s rdna sequencing, clustering with the species pair w. confusa/w. cibaria. the strains were analysed for growth on glucose, xylose and xylooligosaccharides (xos). all strains were xylose positive using the api chl 50 test. growth on xos was observed for strains 85, 92, 145 and av1, firstly by optical density measurements in microtitre plates and secondly in batch cultures also confirming concomitant decrease in ph ...201323738850
characterisation of the spoilage bacterial microbiota in oyster gills during storage at different temperatures.the spoilage bacterial community in oyster gill was investigated during storage at 4, 10 and 20 °c. aerobic plate counts and ph values were determined. total bacterial dna was extracted from oyster gill and bulk cells of plate count media. the major bacterial species during fresh or different temperatures storage were determined by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge).201323696433
taxonomic and molecular characterization of lactic acid bacteria and yeasts in nunu, a ghanaian fermented milk product.produced from raw unpasteurized milk, nunu is a spontaneously fermented yoghurt-like product made in ghana and other parts of west africa. despite the importance of nunu in the diet of many africans, there is currently only limited information available on the microorganisms associated with nunu processing. with the aim of obtaining a deeper understanding of the process and as a first step towards developing starter cultures with desired technological properties for nunu production, a microbiolo ...201223541194
comparison of bacterial community changes in fermenting kimchi at two different temperatures using a denaturing gradient gel electrophoresis analysis.a polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) technique followed by sequencing of the 16s rdna fragments eluted from the bands of interest on denaturing gradient gels was used to monitor changes in the bacterial microflora of two commercial kimchi, salted cabbage, and ingredient mix samples during 30 days of fermentation at 4°c and 10°c. leuconostoc (lc.) was the dominant lactic acid bacteria (lab) over lactobacillus (lb.) species at 4°c. weissella confusa was de ...201323314371
analysis of bacterial diversity during the fermentation of inyu, a high-temperature fermented soy sauce, using nested pcr-denaturing gradient gel electrophoresis and the plate count method.the diversity of bacteria associated with the fermentation of inyu, also known as black soy sauce, was studied through the nested pcr-denaturing gradient gel electrophoresis (dgge) of samples collected from the fermentation stages of the inyu production process. the dgge profiles targeted the bacterial 16s rdna and revealed the presence of citrobacter farmeri, enterobacter cloacae, enterobacter hormaechei, enterococcus faecium, klebsiella pneumoniae, pantoea agglomerans, salmonella enterica, ser ...201323200659
structural analysis of linear mixed-linkage glucooligosaccharides by tandem mass spectrometry.dextrans and glucooligosaccharides (glos) are produced by lactic acid bacteria (lab) during sourdough fermentation. the dextrans can act as hydrocolloids in sourdough bread, while the glos may have antistaling and prebiotic properties, depending on their structure. development of high-throughput methods for screening the structural properties of dextrans and glos produced by different lab in varying fermentation conditions is therefore of interest. in this study we explored the possibility of us ...201323194554
broad-spectrum antifungal-producing lactic acid bacteria and their application in fruit models.a large-scale screen of some 7,000 presumptive lactic acid bacteria (lab), isolated from animal, human, or plant origin, identified 1,149 isolates with inhibitory activity against the food-spoilage mould penicillium expansum. in excess of 500 lab isolates were subsequently identified to produce a broad spectrum of activity against p. expansum, penicillium digitatum, penicillium notatum, penicillium roqueforti, rhizopus stolonifer, fusarium culmorum, aspergillus fumigatus and rhodotorula mucilagi ...201323160868
antifungal activity of leuconostoc citreum and weissella confusa in rice cakes.the antifungal activity of organic acids greatly improves the shelf life of bread and bakery products. however, little is known about the effect of lactic acid fermentation on fungal contamination in rice cakes. here, we show that lactic acid fermentation in rice dough can greatly retard the growth of three fungal species when present in rice cakes, namely cladosporium sp. ys1, neurospora sp. ys3, and penicillium crustosum ys2. the antifungal activity of the lactic acid bacteria against these fu ...201223124754
proteomic analysis of proteins of weissella confusa 31 affected by bile salts.weissella confusa 31, an isolate from human feces, possesses desirable properties as a probiotic strain, including bile salt resistance. w. confusa 31 is not inhibited by bile salts up to 0.3% concentration. proteins affected by bile salts (0.05%) were examined by 2-d gel electrophoresis. our proteomic analyses revealed that the intensities of 29 spots were changed, where 17 increased (including 2 spots observed only under the bile salts stress conditions) and 12 decreased. proteins were identif ...201223075796
characterization of a novel dextransucrase from weissella confusa isolated from sourdough.weissella confusa and weissella cibaria isolated from wheat sourdoughs produce, from sucrose, linear dextrans due to a single soluble dextransucrase. in this study, the first complete gene sequence encoding dextransucrase from a w. confusa strain (lbae c39-2) along with the one from a w. cibaria strain (lbae k39) were reported. corresponding gene cloning was achieved using specific primers designed on the basis of the draft genome sequence of these species. deduced amino acid sequence of w. conf ...201323053097
optimization of electrotransformation conditions for leuconostoc mesenteroides subsp. mesenteroides atcc8293.to establish an efficient genetic transformation protocol for leuconostoc species, methods for competent-cell preparation and electroporation conditions were optimized.201222897881
optimization of exopolysaccharide overproduction by lactobacillus confusus in solid state fermentation under high salinity stress.it is believed that high concentrations of sodium chloride (nacl) suppress the biosynthesis of exopolysaccharide (eps) in lactic acid bacteria (lab). nevertheless, overproduction of epss due to high salinity stress in solid state fermentation performed on an agar surface was demonstrated in this study using a response surface methodology via a central composite design (ccd). under optimized conditions with nacl 4.97% and sucrose 136.5 g/l at 40.79 h of incubation, the eps yield was 259% (86.36 g ...201222738958
complex microbiota of a chinese "fen" liquor fermentation starter (fen-daqu), revealed by culture-dependent and culture-independent methods.daqu is a traditional fermentation starter that is used for chinese liquor production. although partly mechanized, its manufacturing process has remained traditional. we investigated the microbial diversity of fen-daqu, a starter for light-flavour liquor, using combined culture-dependent and culture-independent approaches (pcr-dgge). a total of 190 microbial strains, comprising 109 bacteria and 81 yeasts and moulds, were isolated and identified on the basis of the sequences of their 16s rdna (ba ...201222608236
genotypic characterization and safety assessment of lactic acid bacteria from indigenous african fermented food products.indigenous fermented food products play an essential role in the diet of millions of africans. lactic acid bacteria (lab) are among the predominant microbial species in african indigenous fermented food products and are used for different applications in the food and biotechnology industries. numerous studies have described antimicrobial susceptibility profiles of lab from different parts of the world. however, there is limited information on antimicrobial resistance profiles of lab from africa. ...201222594449
monitoring of the microbial communities involved in the soy sauce manufacturing process by pcr-denaturing gradient gel electrophoresis.soy sauce is a traditional seasoning produced through the fermentation of soybeans and wheat using microbes. in this study, the microbial communities involved in the soy sauce manufacturing process were analyzed by pcr-denaturing gradient gel electrophoresis (pcr-dgge). the bacterial dgge profile indicated that the bacterial microbes in the koji were weissella cibaria (weissella confusa, weissella kimchii, weissella salipiscis, lactobacillus fermentum, lactobacillus plantarum, lactobacillus iner ...201222475947
genome sequence of weissella confusa lbae c39-2, isolated from a wheat sourdough.weissella confusa is a rod-shaped heterofermentative lactic acid bacterium from the family of leuconostocaceae. here we report the draft genome sequence of the strain w. confusa lbae c39-2 isolated from a traditional french wheat sourdough.201222374947
biodiversity of yeasts, lactic acid bacteria and acetic acid bacteria in the fermentation of "shanxi aged vinegar", a traditional chinese vinegar.shanxi aged vinegar is a famous traditional chinese vinegar made from several kinds of cereal by spontaneous solid-state fermentation techniques. in order to get a comprehensive understanding of culturable microorganism's diversity present in its fermentation, the indigenous microorganisms including 47 yeast isolates, 28 lactic acid bacteria isolates and 58 acetic acid bacteria isolates were recovered in different fermenting time and characterized based on a combination of phenotypic and genotyp ...201222265314
Probiotic properties of Weissella strains isolated from human faeces.Three Weissellaconfusa and five Weissellacibaria strains were previously isolated from human faeces and their potential as probiotics was examined in this work. Resistance to low pHs (pH 2.0 and 3.0) and 0.3% bile salt were examined. Enzyme activities, susceptibilities to heat treatment and various antibiotics, and adhesion capacities to Caco-2 cells were also examined. All Weissella strains were killed when exposed to pH 2.0 for 2 h but survived at pH 3.0 with different survival ratios. W. conf ...201122200451
construction of theta-type shuttle vector for leuconostoc and other lactic acid bacteria using pcb42 isolated from kimchi.the pcb42 plasmid from leuconostoc citreum cb2567, a strain isolated from kimchi, was characterized, and a shuttle vector for escherichia coli and lactic acid bacteria (lab) was constructed. the pcb42 plasmid has a circular structure of 4312bp, a low g+c content, and no single-stranded dna intermediates during replication, which indicates that pcb42 replicates via the theta-type replication mechanism. in silico analysis of this plasmid revealed 6 open reading frames: 1 transposase gene, 1 dna-bi ...201222133745
16s rrna-based identification of a glucan-hyperproducing weissella confusa.a gram-positive, nonmotile, irregular, short, rod-shaped new strain of weissella confusa bacterium was isolated from fermented cabbage. the isolate was physiologically and biochemically characterised. the 16s rdna was amplified by polymerase chain reaction (pcr). the isolate was identified as weissella confusa (genbank accession number: gu138518.1) based on nucleotide homology and phylogenetic analysis. the isolate produces glucansucrase when grown in sucrose-supplemented culture medium which ca ...201121603260
weissella confusa: a rare cause of vancomycin resistant gram-positive bacteraemia.we describe a case of bacteraemia caused by weissella confusa in a 48 year old male who was operated for adenocarcinoma of gastro-esophageal junction and maintained on total parenteral nutrition. blood cultures were positive for vancomycin resistant streptococci like organism which was identified as weissella confusa by 16srdna sequencing.201121596906
weissella confusa bacteremia in a liver transplant patient with hepatic artery thrombosis.n.p. harlan, r.r. kempker, s.m. parekh, e.m. burd, d.t. kuhar. weissella confusa bacteremia in a liver transplant patient with hepatic artery thrombosis transpl infect dis 2011: 13: 290-293. all rights reserved abstract: a 54-year-old man with a history of nonalcoholic steatohepatitis and hepatocellular carcinoma presented 2 months after an orthotopic liver transplant with fever and abdominal pain. two weeks earlier, he had an hepatic artery thrombosis and a biliary stricture, for which an hepat ...201021504525
evaluation of the functional potential of weissella and lactobacillus isolates obtained from nigerian traditional fermented foods and cow's intestine.the characterisation of 24 lactic acid bacteria (lab) isolates from nigerian traditional fermented dairy foods, including some cow's intestine isolates, was conducted in order to select isolates for potential use as probiotics. lab isolates were identified by partial sequencing the 16s rrna gene as belonging to the species lactobacillus paracasei, lactobacillus brevis and mainly weissella confusa. at the end of a characterisation process, 2 l. paracasei and 2 w. confusa isolates were selected, a ...201121482440
novel pcr-based identification of weissella confusa using an aflp-derived marker.an extensive use of weissella (w.) confusa is currently being made for the production of a variety of fermented foods and beverages although some strains of this species have emerged as opportunistic pathogens for humans and animals. nevertheless, no rapid methods are available for the reliable identification of w. confusa. we developed a novel pcr using aflp (amplified fragment length polymorphism)-derived primers for the rapid and unequivocal identification of w. confusa. fluorescent aflp of 3 ...201121296447
natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype, 16s ribosomal rna and reca gene analysis.one hundred and fifty-six strains isolated from corn (zea mays l.), forage paddy rice (oryza sativa l.), sorghum (sorghum bicolor l.) and alfalfa (medicago sativa l.) silages prepared on dairy farms were screened, of which 110 isolates were considered to be lactic acid bacteria (lab) according to their gram-positive and catalase-negative characteristics and, mainly, the lactic acid metabolic products. these isolates were divided into eight groups (a-h) based on the following properties: morpholo ...201121282025
analysis of bacterial communities of traditional fermented west african cereal foods using culture independent methods.in this study, the microbial composition of kunu-zaki and ogi, two popular foods in nigeria produced after natural, uncontrolled fermentation of cereals, was assessed by culture-independent molecular profiling methods. in particular, pcr-denaturing gradient gel electrophoresis and construction of 16s rrna gene clone libraries revealed the presence of diverse bacterial communities. dna sequencing of the highly variable v3 region of the 16s rrna genes obtained from pcr-dgge fingerprints identified ...201121272950
structural analysis of enzyme-resistant isomaltooligosaccharides reveals the elongation of α-(1→3)-linked branches in weissella confusa dextran.weissella confusa vtt e-90392 is an efficient producer of a dextran that is mainly composed of α-(1→6)-linked d-glucosyl units and very few α-(1→3) branch linkages. a mixture of the chaetomium erraticum endodextranase and the aspergillus niger α-glucosidase was used to hydrolyze w. confusa dextran to glucose and a set of enzyme-resistant isomaltooligosaccharides. two of the oligosaccharides (tetra- and hexasaccharide) were isolated in pure form and their structures elucidated. the tetrasaccharid ...201121207960
weissella confusa: an unexpected cause of vancomycin-resistant gram-positive bacteremia in immunocompromised hosts.h. salimnia, g.j. alangaden, r. bharadwaj, t.m. painter, p.h. chandrasekar, m.r. fairfax. weissella confusa: an unexpected cause of vancomycin-resistant gram-positive bacteremia in immunocompromised hosts. transpl infect dis 2011: 13: 294-298. all rights reserved abstract: we report the first case of weissella confusa bacteremia in an allogeneic hematopoietic stem cell transplant patient. after engraftment and discharge, the patient returned with fever and graft failure and was started on an emp ...201021156010
bacteraemia caused by weissella confusa at a university hospital in taiwan, 1997-2007.clin microbiol infect abstract: human infections caused by weissella confusa are rarely reported. ten patients with bacteraemia caused by w. confusa who were treated at a tertiary-care hospital in taiwan during 1997-2007 were studied. all isolates were initially misidentified as various lactobacillus and leuconostoc species by two commercial automated identification methods, and were confirmed to be w. confusa by 16s rrna sequencing analysis. mics of these isolates for ten antimicrobial agents w ...201021040157
characterization of dextran-producing weissella strains isolated from sourdoughs and evidence of constitutive dextransucrase expression.the study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. in this study, several dextran-producing strains, weissella cibaria and weissella confusa, isolated from sourdough, were characterized according to carbohydrate fermentation, repetitive element-pcr fingerprinting using (gtg)(5) primers and glucans ...201020722740
solid state fermentation for extracellular polysaccharide production by lactobacillus confusus with coconut water and sugar cane juice as renewable wastes.extracellular polysaccharide (eps) production by lactobacillus confusus in liquid and solid state fermentation was carried out using coconut water and sugarcane juice as renewable wastes. high concentrations of eps of 62 (sugarcane juice) and 18 g/l of coconut water were produced in solid state fermentation when nitrogen sources were reduced 5-fold from the original medium.201020139601
genotypic diversity of lactic acid bacteria isolated from african traditional alkaline-fermented foods.to identify and compare lactic acid bacteria (lab) isolated from alkaline fermentations of cassava (manihot esculenta crantz) leaves, roselle (hibiscus sabdariffa) and african locust bean (parkia biglobosa) seeds for production of, respectively, ntoba mbodi, bikalga and soumbala.201019895650
screening of lactic acid bacteria from indonesia reveals glucansucrase and fructansucrase genes in two different weissella confusa strains from soya.homopolysaccharide (glucan and fructan) synthesis from sucrose by sucrase enzymes in lactic acid bacteria (lab) has been well studied in the genera leuconostoc, streptococcus and lactobacillus. this study aimed to identify and characterize genes encoding glucansucrase/glucosyltransferase (gtf) and fructansucrases/fructosyltransferase (ftf) enzymes from genomic dna of 'rare' indonesian exopolysaccharide-producing lab. from a total of 63 exopolysaccharide-producing lab isolates obtained from foods ...200919758326
in situ production and analysis of weissella confusa dextran in wheat sourdough.several lactic acid bacteria belonging to the genera leuconostoc, lactobacillus, and weissella have been introduced to wheat sourdough baking for in situ production of exopolysaccharides. this is considered a novel method for improving the shelf-life, volume and nutritional value of bread without additives. however, in situ production of exopolysaccharides during sourdough fermentation is challenged by simultaneous acidification due to metabolic activities of the bacteria, which may significantl ...200919747607
lactobacillus and bifidobacterium diversity in horse feces, revealed by pcr-dgge.lactobacillus equi, lactobacillus hayakitensis, lactobacillus johnsonii, and weissella confusa/cibaria were the dominant species in 12 south african horses. the bifidobacterium-group was detected in the feces of only one of the 12 horses. sequencing of the nested-pcr amplicon identified the bifidobacterium-group as parascardovia denticolens. cell numbers of l. equi, l. hayakitensis, and w. confusa/cibaria were consistent in all samples. p. denticolens, bifidodobacterium pseudolongum, and a phylo ...200919730939
polyphasic taxonomic studies of lactic acid bacteria associated with tunisian fermented meat based on the heterogeneity of the 16s-23s rrna gene intergenic spacer region.the objective of this work was to investigate the structure and diversity of lactic acid bacteria (lab) communities in traditionally fermented meat collected from different areas of tunisia. a polyphasic study, which involves phenotypic tests and ribosomal dna-based techniques, was used to identify gram-positive and catalase-negative isolates. pcr amplification of the 16s-23s rdna isr of 102 isolates and other reference lab strains gave (1) one type of rrn operon (m-isr) for lactococci, (2) two ...200919669730
the microbiota of lafun, an african traditional cassava food product.lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ...200919493582
antifungal activity of strains of lactic acid bacteria isolated from a semolina ecosystem against penicillium roqueforti, aspergillus niger and endomyces fibuliger contaminating bakery products.thirty samples of italian durum wheat semolina and whole durum wheat semolina, generally used for the production of southern italy's traditional breads, were subjected to microbiological analysis in order to explore their lactic acid bacteria (lab) diversity and to find strains with antifungal activity. a total of 125 presumptive lab isolates (gram-positive and catalase-negative) were characterized by repetitive extragenic palindromic-pcr (rep-pcr) and sequence analysis of the 16s rrna gene, lea ...200919243908
use of autochthonous starters to ferment red and yellow peppers (capsicum annum l.) to be stored at room temperature.strains of lactobacillus curvatus, leuconostoc mesenteroides, lactobacillus plantarum and weissella confusa were identified from raw red and yellow peppers (ryps) by partial 16s rrna gene sequence and subjected to typing by random amplified polymorphic dna-polymerase chain reaction (rapd-pcr) analysis. l. plantarum pe21, l. curvatus pe4 and w. confusa pe36 were selected based on the kinetics of growth and acidification, and used as the autochthonous mixed starter for the fermentation of ryps. a ...200919217182
taxonomic structure and monitoring of the dominant population of lactic acid bacteria during wheat flour sourdough type i propagation using lactobacillus sanfranciscensis starters.the structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type i propagation by using singly nine strains of lactobacillus sanfranciscensis. under back-slopping propagation with wheat flour type 0 f114, cell numbers of presumptive lactic acid bacteria varied slightly between and within starters. as determined by randomly amplified polymorphic dna-pcr and restriction endonuclease analysis-pulsed-field gel electrophoresis analyses, only ...200919088320
microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge.the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ...200818838186
use of lactobacillus strains to start cassava fermentations for gari production.two lactobacillus strains, lactobacillus plantarum bfe 6710 and lactobacillus fermentum bfe 6620, were used to start cassava fermentations in a pilot study under field production conditions in kenya, to determine their potential to establish themselves as predominant lactobacilli during the fermentation. predominant strains from three fermentations were isolated throughout the 48 h fermentation period. the use of these strains in high numbers clearly resulted in 1 to 2 log higher lactic acid bac ...200818834642
nmr spectroscopic analysis of exopolysaccharides produced by leuconostoc citreum and weissella confusa.dextrans are the main exopolysaccharides produced by leuconostoc species. other dextran-producing lactic acid bacteria include streptococci, lactobacilli, and weissella species. commercial production and structural analysis has focused mainly on dextrans from leuconostoc species, particularly on leuconostoc mesenteroides strains. in this study, we used nmr spectroscopy techniques to analyze the structures of dextrans produced by leuconostoc citreum e497 and weissella confusa e392. the dextrans w ...200818452899
a differential medium for lactic acid-producing bacteria in a mixed culture.modified deman-rogosa sharpe agar containing bromophenol blue (mmrs-bpb) was tested as a medium for counting and differentiation of each lactic acid-producing bacterium (lab), especially in a mixed culture.200818444977
conversion of isoflavone glucosides to aglycones in soymilk by fermentation with lactic acid bacteria.four lactic acid bacteria (lab), lactobacillus paraplantarum km, enterococcus durans kh, streptococcus salivarius hm and weissella confusa jy, were isolated from humans and tested for their capabilities of converting isoflavone glucosides to aglycones in soymilk. changes in growth, ph, and titratable acidity (ta) were investigated during fermentation at 37 degrees c for 12 h. after 6 to 9 h of fermentation, each population of 4 lab reached 10(8) to 10(9) cfu/ml. the initial ph of 6.3 +/- 0.1 dec ...200717995840
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