Publications

environmental impact of tributyltin-resistant marine bacteria in the indigenous microbial population of tributyltin-polluted surface sediments.	we compared the tbt-resistant ability of resting cells prepared from isolates that formed colonies on nutrient agar plates containing 100 µm tributyltin (tbt) chloride, such as photobacterium sp. tky1, halomonas sp. tky2, and photobacterium sp. ngy1, with those from taxonomically similar type strains. photobacterium sp. tky1 showed the highest ability among those three isolates. the number of surviving photobacterium sp. tky1 cells was hardly decreased after 1 h of exposure to 100 µm tbtcl, rega ...	2017	28659560
multiplex genome editing by natural transformation (mugent) for synthetic biology in vibrio natriegens.	vibrio natriegens has recently emerged as an alternative to escherichia coli for molecular biology and biotechnology, but low-efficiency genetic tools hamper its development. here, we uncover how to induce natural competence in v. natriegens and describe methods for multiplex genome editing by natural transformation (mugent). mugent promotes integration of multiple genome edits at high-efficiency on unprecedented time scales. also, this method allows for generating highly complex mutant populati ...	2017	28571309
vibrio natriegens as a fast-growing host for molecular biology.	a rapidly growing bacterial host would be desirable for a range of routine applications in molecular biology and biotechnology. the bacterium vibrio natriegens has the fastest growth rate of any known organism, with a reported doubling time of <10 min. we report the development of genetic tools and methods to engineer v. natriegens and demonstrate the advantages of using these engineered strains in common biotech processes.	2016	27571549
construction of a bioreporter by heterogeneously expressing a vibrio natriegens reca::luxcdabe fusion in escherichia coli, and genotoxicity assessments of petrochemical-contaminated groundwater in northern china.	here, we constructed an escherichia coli reca::luxcdabe bioreporter for genotoxicity assessments. the reca promoter was cloned from the marine bacterium vibrio natriegens. this bioreporter showed a dose-response relationship following induction by mitomycin c, and other pollutants or environmental samples could be calculated as mitomycin c equivalents, which provided a way to quantitatively compare the genotoxicities of different environmental samples. this bioreporter was used to evaluate the g ...	2016	27258332
[screening and identification of a bacterium capable of converting agar to neoagaro oligosaccharides].	to screen and identify a bacterium capable of converting agar to neoagaro oligosaccharides.	2015	26762025
sulfated phenolic acids from dasycladales siphonous green algae.	sulfated aromatic acids play a central role as mediators of chemical interactions and physiological processes in marine algae and seagrass. among others, dasycladus vermicularis (scopoli) krasser 1898 uses a sulfated hydroxylated coumarin derivative as storage metabolite for a protein cross linker that can be activated upon mechanical disruption of the alga. we introduce a comprehensive monitoring technique for sulfated metabolites based on fragmentation patterns in liquid chromatography/mass sp ...	2015	26188914
a novel integrin function in innate immunity from chinese mitten crab (eriocheir sinensis).	integrins belong to a superfamily of conserved α β heterodimeric cell surface receptors that have critical function in cell migration, differentiation, and survival. in this study, an integrin called esintegrin was identified from chinese mitten crab eriocheir sinensis. esintegrin cdna is 4415 bp long with a 2457 bp open reading frame that encodes an 818 amino acid protein. esintegrin contains a signal peptide, an integrin beta subunit (n-terminal portion of extracellular region) inb domain, an ...	2015	26004499
sulfation mediates activity of zosteric acid against biofilm formation.	zosteric acid (za), a metabolite from the marine sea grass zostera marina, has attracted much attention due to its attributed antifouling (af) activity. however, recent results on dynamic transformations of aromatic sulfates in marine phototrophic organisms suggest potential enzymatic desulfation of metabolites like za. the activity of za was thus re-investigated using biofilm assays and simultaneous analytical monitoring by liquid chromatography/mass spectrometry (lc/ms). comparison of za and i ...	2015	25915112
universally improving effect of mixed electron donors on the co₂ fixing efficiency of non-photosynthetic microbial communities from marine environments.	the universality of improved co₂ fixing upon the addition of mixed electron donors (meds) composed of na₂s, no₂(-), and s₂o₃(2-) to non-photosynthetic microbial communities (npmcs) obtained from 12 locations in four oceans of the world was validated. the co₂ fixing efficiencies of npmcs were universally enhanced by med compared with those obtained using h₂ alone as electron donor, with average increase of about 276%. an increase in microbial inoculation concentration could increase the net amoun ...	2014	25108727
function of two novel single-crd containing c-type lectins in innate immunity from eriocheir sinensis.	c-type lectin is one of the pattern-recognition proteins of the non-self-innate immune system in invertebrates. in this study, two novel c-type lectin cdnas (esctl1 and esctl2) of eriocheir sinensis were cloned and characterized. esctl1 has 169 amino acids, whereas esctl2 has 164 amino acids. these two lectins contain one carbohydrate-recognition domain. phylogenetic analysis showed that esctl1 and esctl2 were not clustered with other reported lectins from crabs. esctl1 and esctl2 were expressed ...	2014	24561128
genes similar to the vibrio parahaemolyticus virulence-related genes tdh, tlh, and vscc2 occur in other vibrionaceae species isolated from a pristine estuary.	detection of the human pathogen vibrio parahaemolyticus often relies on molecular biological analysis of species-specific virulence factor genes. these genes have been employed in determinations of v. parahaemolyticus population numbers and the prevalence of pathogenic v. parahaemolyticus strains. strains of the vibrionaceae species photobacterium damselae, vibrio diabolicus, vibrio harveyi, and vibrio natriegens, as well as strains similar to vibrio tubiashii, were isolated from a pristine salt ...	2014	24212573
an ldla domain-containing c-type lectin is involved in the innate immunity of eriocheir sinensis.	c-type lectins (ctls) have crucial functions in recognizing and eliminating pathogens in innate immunity. this study identified a novel low-density lipoprotein receptor class a (ldla) domain-containing ctl, designated as esctldcp, from the chinese mitten crab eriocheir sinensis. the esctldcp cdna is 1258 bp long, with a 975 bp open reading frame that encodes a 324-amino acid protein. esctldcp contains a signal peptide, an ldla, and a single c-type lectin-like domain. esctldcp was only expressed ...	2014	24140432
the mosaic accessory gene structures of the sxt/r391-like integrative and conjugative elements derived from vibrio spp. isolated from aquatic products and environment in the yangtze river estuary, china.	the emergence, resurgence and spread of human food-borne pathogenic vibrios are one of the major contributors to disease burden and mortality particularly in developing countries with disputable sanitary conditions. previous research on pathogenic vibrio cholerae and vibrio parahaemolitycus derived from clinical samples has proposed links between acquisition of virulence and multiple drug resistance traits and intercellular transmissibility of mobile genetic elements in the environment. to date, ...	2013	24074349
draft genome sequence of the fast-growing bacterium vibrio natriegens strain dsmz 759.	vibrio natriegens is a gram-negative bacterium known for its extremely short doubling time. here we present the annotated draft genome sequence of vibrio natriegens strain dsmz 759, with the aim of providing insights about its high growth rate.	2013	23969053
draft genome sequence of the fast-growing marine bacterium vibrio natriegens strain atcc 14048.	vibrio natriegens bacteria are gram-negative aquatic microorganisms that are found primarily in coastal seawater and sediments and are perhaps best known for their high growth rates (generation time of <10 min). in this study, we report the first sequenced genome of this species, that of the type strain vibrio natriegens atcc 14048, a salt marsh mud isolate from sapelo island, ga.	2013	23929482
Production and characterization of a novel monoclonal antibody against Vibrio parahaemolyticus F(0)F(1) ATP synthase's delta subunit and its application for rapid identification of the pathogen.	We raised monoclonal antibodies (MAbs) against Vibrio parahaemolyticus cell extracts. One of the MAbs, designated MAb-VP34, reacted in enzyme-linked immunosorbent assays (ELISAs) with 140 V. parahaemolyticus strains, regardless of serotype or origin. MAb-VP34 did not detectably react with 96 strains belonging to 27 other Vibrio species (except for Vibrio natriegens) or with 29 non-Vibrio species. These results show that MAb-VP34 is highly specific for V. parahaemolyticus. Western blotting and ma ...	2012	22056232
Vibrio jasicida sp. nov., a member of the Harveyi clade, from marine animals (packhorse lobster, abalone, and Atlantic salmon).	Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar) between 1994 and 2002. The bacteria are Gram-negative, rod shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow between 0.5-8.0% NaCl (optimum 3.0-6.0%) and at temperatures of 10-37 °C (optimum 25-30 °C). On the ba ...	2011	21984666
detection of quorum sensing signal molecules in the family vibrionaceae.	the aim of this study was to detect the production of three kinds of quorum sensing (qs) signal molecules, i.e. the n-acyl-homoserine lactone (ahl), the autoinducer-2 (ai-2) and the cholerae autoinducer-1-like (cai-1-like) molecules in 25 vibrionaceae strains.	2011	21395950
[genotypic analysis and plant growth-promoting ability of four plant growth-promoting bacteria from mangrove].	we identified four strains of plant growth-promoting bacteria (pgpb) and their plant growth-promoting ability.	2010	21141471
identification of vibrio natriegens uvra and uvrb genes and analysis of gene regulation using transcriptional reporter plasmids.	nucleotide excision repair (ner) rectifies a variety of chemically and structurally distinct dna lesions. the current model of ner is based upon the enteric bacterium escherichia coli and there is scarce information about how other bacterial species respond to, and correct, dna damage. here we report the isolation and functional analysis of the uvra and uvrb genes from vibrio natriegens, a naturally occurring marine bacterium. genetic studies were completed to assess the repair capabilities of v ...	2010	21046343
2-methoxy-2',4'-dichloro chalcone as an antimicrofoulant against marine bacterial biofilm.	marine paint mixed with 2-methoxy-2',4'-dichloro chalcone is able to considerably reduce the formation of biofilm by vibrio natriegens, a marine bacterium, on polycarbonate (pc), polymethylmethacrylate (pmma) and glass fiber reinforced plastic (gfrp). these polymers have been selected for the study, since they have wide marine applications. surfaces coated with dichloro chalcone containing marine paint had the lowest number of colony forming units (cfu) (1-5×10(6)), proteins (20-30 μg/cm2) and c ...	2010	20708908
antibacterial activity and qsar of chalcones against biofilm-producing bacteria isolated from marine waters.	biofouling in the marine environment is a major problem. in this study, three marine organisms, namely bacillus flexus (ld1), pseudomonas fluorescens (md3) and vibrio natriegens (md6), were isolated from biofilms formed on polymer and metal surfaces immersed in ocean water. phylogenetic analysis of these three organisms indicated that they were good model systems for studying marine biofouling. the in vitro antifouling activity of 47 synthesized chalcone derivatives was investigated by estimatin ...	2010	20544550
marine bacterioplankton can increase evaporation and gas transfer bymetabolizing insoluble surfactants from the air-seawater interface.	hydrophobic surfactants at the air-sea interface can retard evaporative and gaseous exchange between the atmosphere and the ocean.while numerous studies have examined the metabolic role of bacterioneuston at the air-sea interface, the interactions between hydrophobic surfactants and bacterioplankton are not well constrained. a novel experimental design was developed, using vibrio natriegens and (3)h-labelled hexadecanoic acid tracer, to determine how the bacterial metabolism of fatty acids affec ...	2009	19431239
the respiratory system of the marine bacterium beneckea natriegens. i. cytochrome composition.	(1) the cytochrome composition of beneckea natriegens grown under aerobic conditions has been examined. (2) cell-free extracts obtained by sonication were separated into particulate and supernatant fractions by centrifugation at 150,000 x g. (3) the particulate fraction contained cytochromes b562, b557, b or c554, c549.5, c547, and low concentrations of cytochromes a1 and a2. (subscripts refer to the wavelength optima of the b and c type cytochrome alpha-peaks in low temperature (77 degrees k) d ...	1974	19400035
specific atp and specific oxygen uptake rate in immobilized cell aggregates: experimental results and theoretical analysis using a structured model of immobilized cell growth.	in this work the quality and activity of immobilized beneckea natriegens have been measured using specific atp (satp) [mg (atp) g(-1) (dry biomass)] and specific oxygen uptake rate (sour) [mg o(2) g(-1) (dry biomass) h(-1)]. the cells were grown in a 3 l three phase air lift bioreactor (tpalb) and were immobilized on diatomaceous earth (silica) support particles; sterile conditions were employed during the experiment, with n-propanol as the sole carbon source. two sets of experiments were perfor ...	1997	18636576
effects of ultraviolet radiation on the gram-positive marine bacterium microbacterium maritypicum.	although extensive information is available on the effect ultraviolet (uv) radiation has on gram-negative marine bacteria, there is a scarcity of data concerning uv radiation and gram-positive marine bacteria. the focus of this paper is on microbacterium maritypicum, with the gram-negative vibrio natriegens being used as a standard of comparison. m. maritypicum exhibited growth over a nacl range of 0-1000 mm: , with optimum growth occurring between 0 and 400 mm: nacl. in contrast, v. natriegens ...	2007	17551790
production of poly-beta-hydroxybutyrate (phb) by vibrio spp. isolated from marine environment.	bacteria isolated from marine sediments were screened for their ability to accumulate polyhydroxyalkanoates. among the isolates, four vibrio spp. (strain m11, m14, m20 and m31) were studied in detail. all synthesized intracellular lipid inclusions during growth on diverse carbon sources including acetate, glycerol, succinate, glucose and sucrose. the inclusions were identified to be poly-beta-hydroxybutyrate (phb) using gas chromatography and nuclear magnetic resonance analysis. no other type of ...	2007	17416432
plasticity of the gene functions for dna replication in the t4-like phages.	we have completely sequenced and annotated the genomes of several relatives of the bacteriophage t4, including three coliphages (rb43, rb49 and rb69), three aeromonas salmonicida phages (44rr2.8t, 25 and 31) and one aeromonas hydrophila phage (aeh1). in addition, we have partially sequenced and annotated the t4-like genomes of coliphage rb16 (a close relative of rb43), a. salmonicida phage 65, acinetobacter johnsonii phage 133 and vibrio natriegens phage nt-1. each of these phage genomes exhibit ...	2006	16828113
pyridine nucleotide-linked reactions of pseudomonas natriegens.	eagon, r. g. (university of georgia, athens). pyridine nucleotide-linked reactions of pseudomonas natriegens. j. bacteriol. 84:819-821. 1962-the observation that pseudomonas natriegens utilizes the embden-meyerhof pathway and the hexose monophosphate-pentose cycle only very slightly, even though the necessary enzymes are present, was explained by the existence of a sluggish system for the oxidation of reduced triphosphopyridine nucleotide (tpnh). pyridine nucleotide transhydrogenase could not be ...	1962	16561966
fluorescently labeled virus probes show that natural virus populations can control the structure of marine microbial communities.	fluorescently stained viruses were used as probes to label, identify, and enumerate specific strains of bacteria and cyanobacteria in mixed microbial assemblages. several marine virus isolates were fluorescently stained with yoyo-1 or popo-1 (molecular probes, inc.) and added to seawater samples that contained natural microbial communities. cells to which the stained viruses adsorbed were easily distinguished from nonhost cells; typically, there was undetectable binding of stained viruses to nat ...	1995	16535146
major products of glucose dissimilation by pseudomonas natriegens.	eagon, r. g. (university of georgia, athens), and h. w. cho. major products of glucose dissimilation by pseudomonas natriegens. j. bacteriol. 89:1209-1211. 1965.-pseudomonas natriegens aerobically catabolized glucose to yield predominantly acetic acid, pyruvic acid, and co(2), whereas little or no lactic acid was formed. under anaerobic conditions, glucose in an enriched medium was fermented to yield acetic acid and lactic acid but no pyruvic acid or co(2). glucose in a basal salts medium was fe ...	1965	14292987
lactic dehydrogenases of pseudomonas natriegens.	walker, hazel (university of georgia, athens), and r. g. eagon. lactic dehydrogenases of pseudomonas natriegens. j. bacteriol. 88:25-30. 1964.-lactic dehydrogenases specific for d- and l-lactate were demonstrated in pseudomonas natriegens. the l-lactic dehydrogenase showed considerable heat stability, and 40% of the activity remained in extracts after heating at 60 c for 10 min. an essential thiol group for enzyme activity was noted. the results of these experiments were consistent with the view ...	1964	14197895
pseudomonas natriegens, a marine bacterium with a generation time of less than 10 minutes.	eagon, r. g. (university of georgia, athens). pseudomonas natriegens, a marine bacterium with a generation time of less than 10 minutes. j. bacteriol. 83:736-737. 1962.-pseudomonas natriegens, a marine microorganism, was demonstrated to have a generation time of 9.8 min. this is the shortest generation time reported to date. optimal growth occurred at 37 c in brain heart infusion broth supplemented with 1.5% sea salt.	1962	13888946
dissimilation of glucose and gluconic acid by pseudomonas natriegens.	eagon, r. g. (university of georgia, athens) and c. h. wang. dissimilation of glucose and gluconic acid by pseudomonas natriegens. j. bacteriol. 83:879-886. 1962-when glucose dissimilation of a marine pseudomonad, pseudomonas natriegens, was studied, enzymes of both the glycolytic pathway and of the hexose monophosphate pathway were detected in extracts of glucose-grown cells. enzymes of the entner-doudoroff pathway and phosphoketolase were not detected. data from radiorespirometric experiments ...	1962	13888944
some observations on the physiology of pseudomonas natriegens nov. spec.		1961	13733692
[biosynthesis and accumulation of poly(3-hydroxybutyrate) in vibrio natriegens].	accumulation of poly(3-hydroxybutyrate) [poly(3hb)] by v. natriegens was studied. results indicated that v. natriegens used glucose, gluconate, fructose and molasses as carbon sources for poly(3hb) synthesis. when molasses was used, up to 28.4% of poly(3hb) to cellular dry weight was accumulated. the accumulation of poly(3hb) followed, was not simultaneously to, the cell growth. analysis of the pha polymerase, beta-ketothiolase, and acetoacetyl-coa reductase showed that the poly(3hb) accumulatio ...	2002	12561210
estimation of biologically damaging uv levels in marine surface waters with dna and viral dosimeters.	we have surveyed the biologically harmful radiation penetrating the water column along a transect in the western gulf of mexico using dosimeters consisting of intact viruses or naked calf-thymus dna (ctdna). the indigenous marine bacteriophage pwh3a-p1, which lytically infects the heterotrophic bacterium vibrio natriegens (strain pwh3a), displayed decay rates for infectivity approaching 1.0 h(-1) in surface waters when deployed in a seawater-based dosimeter. the accumulation of pyrimidine dimers ...	2002	12403447
reca expression in response to solar uvr in the marine bacterium vibrio natriegens.	solar ultraviolet radiation may produce daily stress on marine and estuarine communities as cells are damaged and repair that damage. reduction in the earth's stratospheric ozone layer has increased awareness of the potential effects that ultraviolet radiation may have in the environment, including how marine bacteria respond to changes in solar radiation. we examined the use of the bacterial reca protein as an indicator of the potential of bacteria to repair dna damage caused by solar uv irradi ...	2001	12024236
rrna promoter activity in the fast-growing bacterium vibrio natriegens.	the bacterium vibrio natriegens can double with a generation time of less than 10 min (r. g. eagon, j. bacteriol. 83:736-737, 1962), a growth rate that requires an extremely high rate of protein synthesis. we show here that v. natriegens' high potential for protein synthesis results from an increase in ribosome numbers with increasing growth rate, as has been found for other bacteria. we show that v. natriegens contains a large number of rrna operons, and its rrna promoters are extremely strong. ...	2002	11844764
n2 fixation in marine heterotrophic bacteria: dynamics of environmental and molecular regulation.	molecular and immunological techniques were used to examine n2 fixation in a ubiquitous heterotrophic marine bacterium, the facultative anaerobic vibrio natriegens. when batch cultures were shifted from aerobic n-replete to anaerobic n-deplete conditions, transcriptional and post-translational regulation of n2 fixation was observed. levels of nifhdk mrna encoding the nitrogenase enzyme were highest at 140 min postshift and undetectable between 6 and 9 h later. immunologically determined levels o ...	1996	11607653
fourier transform-infrared spectroscopic methods for microbial ecology: analysis of bacteria, bacteria-polymer mixtures and biofilms.	fourier transform-infrared (ft-ir) spectroscopy has been used to rapidly and nondestructively analyze bacteria, bacteria-polymer mixtures, digester samples and microbial biofilms. diffuse reflectance ft-ir (drift) analysis of freeze-dried, powdered samples offered a means of obtaining structural information. the bacteria examined were divided into two groups. the first group was characterized by a dominant amide i band and the second group of organisms displayed an additional strong carbonyl ...	1985	11539050
structures of ribonuclease p rnas of vibrio core species.	the structures of an rna component of ribonuclease p (rnase p rna) were examined for vibrio parahaemolyticus, vibrio alginolyticus, vibrio carchariae, vibrio natriegens, vibrio campbellii, vibrio proteolyticus, vibrio pelagius and vibrio harveyi to clearly determine their genetic differences. the rnase p rnas ranged from 382 to 454 nucleotides (nt) in size, and were remarkably different from each other in the structure of two helices, p3 and p12. the p3 helices were comprised of tandem repeats o ...	2001	11430405
effects of bacteria on the growth of an amoeba infecting the gills of turbot.	we analysed the influence of various bacteria on the in vitro growth of trophozoites of a platyamoeba strain isolated from diseased gill tissues of cultured turbot. little or no growth was shown by amoebae cultured in the presence of (1) the turbot-pathogenic bacteria vibrio anguillarum, aeromonas salmonicida or streptococcus sp., (2) pasteurella piscicida or vibrio vulnificus (pathogenic for some fishes but not turbot), or (3) the non-pathogenic 'environmental' bacteria vibrio campbelli, vibrio ...	2001	11411647
grazing characteristics and growth efficiencies at two different temperatures for three nanoflagellates fed with vibrio bacteria at three different concentrations.	small inocula of one of the flagellates paraphysomonas imperforata, pteridomonas danica, and cafeteria roenbergensis were added to suspensions of the bacterium vibrio natriegens at each of three concentrations between 107 and 108 cells ml-1 and incubated at each of the temperatures 10 degrees c and 25 degrees c. samples were taken at intervals for counting the flagellates and bacteria to determine the timing of the maximum of flagellate numbers and the concentrations at that time. measurements o ...	2001	11391464
vibrio pelagius: differences of the type strain deposited at various culture collections.	a critical evaluation of published and own taxonomic and phylogenetic studies on vibrio pelagius showed substantial diversity of strains received as type strains from various culture collections. the comparison of data based upon 16s rrna sequence analyses, earlier genomic dna-dna similarity studies as well as physiological investigations and the original description indicate that vibrio pelagius strains cect 4202t and atcc 25916t really represent the originally described type species whereas st ...	2000	11108016
biofilm development in a membrane-aerated biofilm reactor: effect of flow velocity on performance.	the effect of liquid flow velocity on biofilm development in a membrane-aerated biofilm reactor was investigated both by mathematical modeling and by experiment, using vibrio natriegens as a test organism and acetate as carbon substrate. it was shown that velocity influenced mass transfer in the diffusion boundary layer, the biomass detachment rate from the biofilm, and the maximum biofilm thickness attained. values of the overall mass transfer coefficient of a tracer through the diffusion bound ...	2000	10620763
marine bacterial isolates display diverse responses to uv-b radiation.	the molecular and biological consequences of uv-b radiation were investigated by studying five species of marine bacteria and one enteric bacterium. laboratory cultures were exposed to an artificial uv-b source and subjected to various post-uv irradiation treatments. significant differences in survival subsequent to uv-b radiation were observed among the isolates, as measured by culturable counts. uv-b-induced dna photodamage was investigated by using a highly specific radioimmunoassay to measur ...	1999	10473381
the inorganic ion content of native aquatic bacteria.	in this study we have quantified the ionic content and volume of native aquatic, and two cultured bacteria, by x-ray microanalysis (xrma) in the transmission electron microscope (tem). the cellular concentrations of magnesium (means of 630 and 710 mm) were more than an order of a magnitude higher than the outside concentrations. the internal concentrations of sodium were on average 50-180 mm, and the [k+]/[na+] ratios were in the range of 0.1-0.5; lowest for apparently nonactive bacteria. magnes ...	1999	10420582
photoreactivation compensates for uv damage and restores infectivity to natural marine virus communities.	we investigated the potential for photoreactivation to restore infectivity to sunlight-damaged natural viral communities in offshore (chlorophyll a, < 0.1 microgram liter-1), coastal (chlorophyll a, ca. 0.2 microgram liter-1), and estuarine (chlorophyll a, ca. 1 to 5 micrograms liter-1) waters of the gulf of mexico. in 67% of samples, the light-dependent repair mechanisms of the bacterium vibrio natriegens restored infectivity to natural viral communities which could not be repaired by light-ind ...	1997	9172339
distribution of viral abundance in the reef environment of key largo, florida.	the distribution of viral and microbial abundance in the key largo, fla., reef environment was measured. viral abundance was measured by transmission electron microscope direct counts and plaque titer on specific bacterial hosts in water and sediment samples from florida bay (blackwater sound) and along a transect from key largo to the outer edge of the reef tract in key largo sanctuary. water column viral direct counts were highest in blackwater sound of florida bay (1.2 x 10(7) viruses per ml) ...	1993	8480998
antimicrobial activity of essential oil from schinus molle linn.	the essential oil from the fresh leaves of schinus molle isolated by hydrodistillation was tested for antibacterial activity using the hole plate diffusion method and for antifungal activity using the mycelium or single cell growth inhibition method. results obtained showed that the volatile oil exhibited significant activity against the following bacterial species: klebsiella pneumoniae, alcaligenes faecalis, pseudomonas aeruginosa, leuconostoc cremoris, enterobacter aerogenes, proteus vulgaris ...	1993	8055554
ticarcillin bioassay.	an accurate, plate diffusion bioassay for ticarcillin, utilizing the fast-replicating beneckea natriegens and 4% salt agar, is described in this report. zones of inhibition were well defined after 3 h, and the limit of sensitivity was around 5.0 mug/ml. the assay is simple to carry out, and duplicate assays can be performed on as little as 40 mul of serum.	1981	6979305
relationship between ion requirements for respiration and membrane transport in a marine bacterium.	intact cells of the marine bacterium alteromonas haloplanktis 214 oxidized nadh, added to the suspending medium, by a process which was stimulated by na+ or li+ but not k+. toluene-treated cells oxidized nadh at three times the rate of untreated cells by a mechanism activated by na+ but not by li+ or k+. in the latter reaction, k+ spared the requirement for na+. intact cells of a. haloplanktis oxidized ethanol by a mechanism stimulated by either na+ or li+. the uptake of alpha-aminoisobutyric ac ...	1984	6690427
amp metabolism in the marine bacterium beneckea natriegens.	the catabolism of amp by preparations from beneckea natriegens has been reexamined. in the absence of atp, cell-free extracts catabolized amp via adenosine to inosine. when atp was present, adenylate kinase converted amp to adp, lowering the rate of amp catabolism. particle-free supernatants (225,000 x g) metabolized amp alone slowly, but adenylate kinase was active when atp was added. washed particulate fractions contained amp nucleotidase activity which converted amp to adenosine; in the prese ...	1980	6249477
influence of cations on spheroplasts of marine bacteria functioning as osmometers.	penetration of substrates into marine bacteria as influenced by cations has been demonstrated by the effects of increased osmotic pressure in spheroplasts of these cells. spheroplasts of pseudomonas natriegens, stabilized with lactose, underwent a metabolic swelling in the presence of a substrate to which they had been induced. maximal and persistent swelling was achieved only by addition of catabolizable substrate and both na(+) and k(+). addition, along with substrate, of na(+) alone or k(+) a ...	1967	6035044
substrate binding by crude membranes and solubilized membrane extracts from pseudomonas natriegens.		1968	5305782
the case for the proposed increase in iron enrichment of flour and wheat products.		2015	5023246
pseudosarcoma of the skin. an electron microscopic study and comparison with the fine structure of spindle-cell variant of squamous carcinoma.		2016	4819223
the kidney in sickle cell disease ii: the kidney and sickling phenomenon.		2016	4805648
in vitro studies on the control of trophoblast outgrowth in the mouse.		1973	4729947
ly-4, a new locus determining a lymphocyte cell-surface alloantigen in mice.	a new locus is described that determines an alloantigen on the surface of lymphocytes. it differs from loci previously described in that the corresponding antibody, at least in most antisera, reacts almost exclusively with node lymphocytes, and weakly or not at all with thymuslymphocytes. positive strains include c57bl/6, c57bl/10, c57l, c57br/cd, and rf; negative strains include balb/c, c3h, and sjl. the symbol ly-4 is assigned, with the c57bl/6 allele being ly-4(b), and the balb/c allele ly-4( ...	2015	4515607
what is behind our masks?		2015	4495375
[transvaal pedagogic discussion group. the ward nurse as a pedagogue].		2015	4483653
[case of pie (pulmonary infiltrate with eosinophilia) syndrome caused by candida].		2015	4477881
factors affecting the pathways of glucose catabolism and the tricarboxylic acid cycle in pseudomonas natriegens.	less than 50% of theoretical oxygen uptake was observed when glucose was dissimilated by resting cells of pseudomonas natriegens. low oxygen uptakes were also observed when a variety of other substrates were dissimilated. when uniformly labeled glucose-(14)c was used as substrate, 56% of the label was shown to accumulate in these resting cells. this material consisted, in part, of a polysaccharide which, although it did not give typical glycogen reactions, yielded glucose after its hydrolysis. r ...	1967	4381634
virus infections of horses at newmarket, 1972 and 1973.		1974	4375339
the respiratory system of the marine bacterium beneckea natriegens. oxidation--reduction potentials of the cytochromes.		1974	4369352
a soluble co-binding c-type cytochrome from the marine bacterium beneckea natriegens.		1973	4352552
influence of na+ on synthesis of macromolecules by a marine bacterium.	resting cells of vibrio natriegens acquired the ability to take up (14)c-labeled mannitol in media containing na(+) and k(+). but, the cells took up a significant quantity of the label as well in the presence of 0.3 m k(+) and no na(+). the label was distributed throughout the cells in both systems. cells incubated in mannitol minimal culture medium proliferated and synthesized approximately nine times as much protein in the presence of na(+) and k(+) as those incubated in the presence of mannit ...	1971	4327612
effects of chloroquine pretreatment on lethal dose of morphine and on morphine metabolism in rats.		1969	4309711
[inhibition of the lipolytic activity of noradrenaline by acidosis].		1966	4228153
influence of ototoxic drugs on acetylcholine-induced depression of the cochlear n1 potential.		1970	4195594
[morphogenetic and biochemical criteria for the differentiation of the genera nocardia and mycobacterium].		1965	4183822
the respiratory system of the marine bacterium beneckea natriegens. ii. terminal branching of respiration to oxygen and resistance to inhibition by cyanide.		1974	4154106
beating duration of cultured rat heart cells as affected by drugs and other factors.		1974	4139725
isolation of bacteriophages of the marine bacterium beneckea natriegens from coastal salt marshes.	bacteriophages of the marine bacterium beneckea natriegens were isolated from coastal marshes where they were limited to brackish and marine waters. the phages were widely distributed and morphologically diverse in the marshes.	1974	4133830
[preparation and purification of anti-ferritin, anti-cytochrome b 5 hybrid antibodies].		2014	4122927
some acoustic evidence for vocal abuse in adult speakers with repaired cleft palate.	acoustic evidence for vocal abuse in adult speakers with cleft palate and hypernasality has not been reported. this study was undertaken to determine if there was acoustic evidence of inappropriate vocal fold adduction during stop consonant production in this population. the results indicated that spectrograms of speakers without cleft palate and severe hypernasality exhibited acoustic characteristics different from spectrograms of speakers without cleft palate and speakers with cleft palate and ...	2014	4010425
[conservative treatment of injuries of the liver].		2014	3911104
[a comparative study. the older person too is an individual].		2013	3850215
infusion of amino acids decreases the hyperglycaemic effect of per- and postoperative glucose infusion: an experimental study in the rabbit.	the effects on blood glucose concentration of 10% glucose with amino acid solution at low (18%) and high (25%) concentration of branched chain amino acids were studied, two days after laparotomy in fasted rabbits. during the whole study period, saline infusion was associated with normoglycaemia. among the other infusion combinations peroperative infusion of amino acid solution high in branched chain amino acids with 10% glucose was the least hyperglycaemic. an amino acid solution low in branched ...	1986	3751608
[the problem with the occurrence of so-called spontaneous ruptures of the rectum in horses. 2: forensic evaluation].	in the ampulla roof of the wave-like extending horse rectum the muscular coat is only weakly developed as a deltoid-shaped tunica muscularis area, thus forming a place of minor resistance. with regard to the degree of development of the muscular coat a rectal ampulla with a stable form and strong muscles can be distinguished from an ampulla with a labile form and weak muscles. the rupture of the intestinal wall in this region in the case of rupture-experiments as well as the frequent occurrence ...	1986	3738898
endotracheal resuscitation of preterm infants at birth.		2013	3688937
thymic involvement in acute disseminated histiocytosis x.		2013	3628861
thyroglobulin and 131i uptake of remaining tissue in patients with differentiated carcinoma after thyroidectomy.	in 158 thyroidectomized patients with well-differentiated non-metastatic thyroid cancer the results of serum thyroglobulin (tg) determination and 131i uptake values established immediately before radioiodine treatment (19 days after surgery) were compared. in 113 patients (72%) tg was above 6 ng ml-1 (lower limit of detection) and 131i uptake exceeded 2%. in 11 patients (7%) tg was undetectable and 131i uptake less than 2%. in these cases a radioiodine treatment was not performed; the thyroid ab ...	2012	3399222
the piriformis syndrome.		2012	3390766
oxidation of antipsoriatic 10-acyldithranol derivatives and dithranol to 1,8-dihydroxyanthraquinone.		2012	3369924
structure determination of cryptococcus neoformans serotype a-variant glucuronoxylomannan by 13c-n.m.r. spectroscopy.	a series of polysaccharides was derived by physical and chemical methods from an antigenic, o-acetyl-containing, glucuronoxylomannan (gxm), isolated from the growth medium of cryptococcus neoformans (cdc b2550) serotype a-variant having composition ratios of man:xyl:glca:oac = 10:4:3:6. 13c-n.m.r. spectra of derivatives provided new structural evidence for gxm. treatment of gxm with li in ethylenediamine gave a xylomannan (xm, with man:xyl = 5:2). smith degradation of xm gave a mannan (m). ultra ...	1988	3280130
[duodenal gastrinoma. description of a clinical case].		2011	3231322
rocky mountain spotted fever--united states, 1987.		2011	3131657
valproate-associated hepatotoxicity and its biochemical mechanisms.	intake of the anticonvulsant drug valproic acid, or its sodium salt, has been associated with occasional instances of severe and sometimes fatal hepatotoxicity. probably at least 80 cases have occurred worldwide. the syndrome affects perhaps 1 in 10,000 persons taking the drug, and usually develops in the early weeks or months of therapy. most instances have involved children, usually those receiving more than 1 anticonvulsant. multiple cases have occurred in 2 families. the typical presentation ...	2011	3131628
[enterostoma therapy--a pilot course in the essen education center of the german nursing association].		2011	3127632
biosynthesis and processing of platelet gpiib-iiia in human megakaryocytes.	platelet membrane glycoprotein iib-iiia forms a calcium-dependent heterodimer and constitutes the fibrinogen receptor on stimulated platelets. gpiib is a two-chain protein containing disulfide-linked alpha and beta subunits. gpiiia is a single chain protein. these proteins are synthesized in the bone marrow by megakaryocytes, but the study of their synthesis has been hampered by the difficulty in obtaining enriched population of megakaryocytes in large numbers. to examine the biosynthesis and pr ...	1987	3108266
dice: nonclinical causes of overtreatment.		2010	2916481
exclusion testing for huntington's disease in pregnancy with a closely linked dna marker.	55 couples where one partner was at 50% risk of huntington's disease (hd) were investigated with a dna probe closely linked to hd, with a view to exclusion testing in a future pregnancy. in 3 of 9 pregnancies so far, hd was excluded in the absence of recombination. in 3 the risk was raised to around 50%, and in 2 exclusion tests were uninformative. the remaining couple changed their minds about termination of the pregnancy and the test was therefore judged inappropriate.	2010	2884411
[biochemical aspect in cardiac ischemia--ischemic changes and reperfusion injury].		2010	2838873
[current empirical data on conditions in relation to death in the hospital].	this survey-study represents the psycho-social conditions of death in west-german hospitals as seen from different occupational groups. the test, developed for this task, has been applied in 70 hospitals of different federal states, medical-departments and supporters. it is shown that following factors have an essential influence on the attitudes of people to the needs of the dying patient: amount of medical engineering at ward, time of professional activity and status of the personnel. in sum, ...	2010	2798718
urinary kallikrein excretion during potassium chloride infusion in potassium-adapted rats: effect of amiloride.	1. urinary kallikrein excretion in the anaesthetized rat was measured during intravenous kcl infusion in control and in k+-adapted rats. 2. the infusion of 0.1 mol/l kcl at 3.0 ml/h for 60 min in control rats resulted in a significant increase in urinary kallikrein excretion, associated with diuresis, natriuresis and kaliuresis. 3. when rats were offered 0.1 mol/l kcl to drink ad libitum for 14 days (k+-adaptation), the basal excretion of kallikrein was higher than in the control rats. in k+-ada ...	1989	2758759
dopamine receptors in rabbit and rat eye: characterization and localization of da1 and da2 binding sites.	in vitro autoradiography is used to characterize and localize da1 and da2 receptors in rabbit and rat eyes. the bindings of 125i-sch 23982 (da1 antagonist) and of 125i-iodosulpride (da2 antagonist) to slide mounted tissue sections take place with characteristics expected of substances that recognize da1 and da2 receptors respectively. they are saturable, have high affinity and exhibit an appropriate pharmacology. the regional distribution of these receptors indicates that they are mainly present ...	1989	2707038
[therapy monitoring of regional chemotherapy by magnetic resonance spectroscopy].		2008	2474953