Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the role for the small cryptic plasmids as moldable vectors for genetic innovation in aeromonas salmonicida subsp. salmonicida. | in aeromonas salmonicida subsp. salmonicida, a bacterium that causes fish disease, there are two types of small plasmids (<15 kbp): plasmids without known function, called cryptic plasmids, and plasmids that bear beneficial genes for the bacterium. four among them are frequently detected in strains of a. salmonicida subsp. salmonicida: pasa1, pasa2, pasa3, and pasal1. the latter harbors a gene which codes for an effector of the type three secretion system, while the three others are cryptic. it ... | 2017 | 29326751 |
| genomic and phenotypic characterization of an atypical aeromonas salmonicida strain isolated from a lumpfish and producing unusual granular structures. | aeromonas salmonicida strains are roughly classified into two categories, typical and atypical strains. the latter mainly regroup isolates that present unusual phenotypes or hosts, comparatively to the typical strains that belong to the salmonicida subspecies. this study focuses on an uncharacterized atypical strain, m18076-11, isolated from lumpfish (cyclopterus lumpus) and not part of the four recognized aeromonas salmonicida subspecies. this isolate presents an unreported phenotype in the a. ... | 2018 | 29315617 |
| the complete and fully assembled genome sequence of aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other aeromonas species: investigation of the mobilome in environmental and pathogenic strains. | due to the predominant usage of short-read sequencing to date, most bacterial genome sequences reported in the last years remain at the draft level. this precludes certain types of analyses, such as the in-depth analysis of genome plasticity. | 2018 | 29304740 |
| epidemiology of danish aeromonas salmonicida subsp. salmonicida in fish farms using whole genome sequencing. | furunculosis, a serious infection caused by the bacterium aeromonas salmonicida subsp. salmonicida is common in sea-reared rainbow trout production in denmark. developing an effective control strategy requires knowledge of the epidemiology, as well as the genomic and virulent variability of the danish a. salmonicida subsp. salmonicida isolates. to obtain this, the genomes of 101 a. salmonicida subsp. salmonicida, including 99 danish isolates, one scottish strain and the type strain ncimb 1102, w ... | 2017 | 29259599 |
| vaccination of atlantic lumpfish (cyclopterus lumpus l.) at a low temperature leads to a low antibody response against aeromonas salmonicida. | we present a study on the effect of water temperature on immunization of atlantic lumpfish. in total, 360 fish were vaccinated with either 50 μl of an oil-based injection vaccine (vax), with aeromonas salmonicida and vibrio salmonicida antigens, or pbs. fish were vaccinated at three different water temperatures, 5°c, 10°c and 15°c, and sorted into six groups (n = 60). lumpfish were weighed every 3 weeks after vaccination, sampled at 3, 6, 9 and 18 weeks post-immunization (wpi) and evaluated by m ... | 2017 | 29226986 |
| in vivo selection of a multidrug-resistant aeromonas salmonicida during medicinal leech therapy. | we report the selection in a 15-year-old boy of a multidrug-resistant, extended-spectrum β-lactamase (esbl)-producing aeromonas salmonicida after medicinal leech therapy that required an antibiotic prophylaxis based on piperacillin/tazobactam and cotrimoxazole. whole genome sequencing of the strain indeed revealed 13 antibiotic resistance genes, including the esbl ctx-m-3 and the unusual β-lactamase sco-1. | 2018 | 29204282 |
| first isolation of vibrio tapetis and an atypical strain of aeromonas salmonicida from skin ulcerations in common dab (limanda limanda) in the north sea. | skin ulcerations rank amongst the most prevalent lesions affecting wild common dab (limanda limanda) with an increase in prevalence of up to 3.5% in the belgian part of the north sea. a complex aetiology of these ulcerations is suspected, and many questions remain on the exact factors contributing to these lesions. to construct the aetiological spectrum of skin ulcerations in flatfish, a one-day monitoring campaign was undertaken in the north sea. fifteen fish presented with one or more ulcerati ... | 2018 | 29148593 |
| interplay between daily rhythmic serum-mediated bacterial killing activity and immune defence factors in rainbow trout (oncorhynchus mykiss). | circadian rhythm is emerging as an important regulator of immune functions. however, there is a paucity of information on the influence of this biological phenomenon in the antimicrobial factors in teleost fish. this study investigated the dynamics and interplay of serum-mediated bacterial killing activity and immune defence factors throughout the light:dark (ld) cycle in rainbow trout (oncorhynchus mykiss). the juvenile fish came from two different emergence time fractions (i.e., late and early ... | 2018 | 29146445 |
| study of mesophilic aeromonas salmonicida a527 strain sheds light on the species' lifestyles and taxonomic dilemma. | the gram-negative bacterium aeromonas salmonicida contains five subspecies: salmonicida, smithia, achromogenes, masoucida and pectinolytica. pectinolytica is a mesophilic subspecies with the ability to thrive at a wide range of temperatures, including 37°c, while the four other subspecies are psychrophilic, restricted to lower temperatures. the psychrophilic subspecies are known to infect a wide range of fishes. however, there is no evidence of pathogenicity for the mesophilic subspecies pectino ... | 2017 | 29126137 |
| inducers of salmon innate immunity: an in vitro and in vivo approach. | maintaining fish health is one of the most important aims in aquaculture. prevention of fish diseases therefore is crucial and can be achieved by various different strategies, including most often a combination of different methods such as optimal feed and fish density, as well as strengthening the immune system. understanding the fish innate immune system and developing methods to activate it, in an effort to prevent infections in the first place, has been a goal in recent years. in this study ... | 2018 | 29108970 |
| identification of a moronecidin-like antimicrobial peptide in the venomous fish pterois volitans: functional and structural study of pteroicidin-α. | the present study characterizes for the first time an antimicrobial peptide in lionfish (pterois volitans), a venomous fish. using a peptidomic approach, we identified a mature piscidin in lionfish and called it pteroicidin-α. we detected an amidated form (pteroicidin-α- conh2) and a non-amidated form (pteroicidin-α-cooh), and then performed their functional and structural study. interestingly, the two peptides displayed different antibacterial and hemolytic activity levels. pteroicidin-α-conh2 ... | 2018 | 29108968 |
| characterization and functional assessment of the nlrc3-like molecule of the goldfish (carassius auratus l.). | the nlrc3-like (nlrc3l) molecule from the goldfish transcriptome database was identified and characterized. quantitative gene expression analysis revealed the highest mrna levels of nlrc3l were in the spleen and intestine, with lower mrna levels observed in muscle and liver. goldfish nlrc3l was differentially expressed in goldfish immune cell populations with highest mrna levels measured in pbls and macrophages. we generated a recombinant form of the molecule (rgfnlrc3l) and an anti-ct-nlrc3l ig ... | 2018 | 28988993 |
| draft genome sequence of the type strain aeromonas salmonicida subsp. salmonicida atcc 33658. | here, we report the draft genome sequence of the type strain aeromonas salmonicida subsp. salmonicida atcc 33658 isolated from salmo salar the size of the genome is 4,728,143 bp with a g+c content of 58.5%. the a. salmonicida subsp. salmonicida atcc 33658 genome lacks essential virulence genes that were likely lost during genomic rearrangements. | 2017 | 28982999 |
| microplastics as a vector for the transport of the bacterial fish pathogen species aeromonas salmonicida. | microplastics is widespread in the marine environment where it can cause numerous negative effects. it can provide space for the growth of organisms and serves as a vector for the long distance transfer of marine microorganisms. in this study, we examined the sea surface concentrations of microplastics in the north adriatic and characterized bacterial communities living on the microplastics. dna from microplastics particles was isolated by three different methods, followed by pcr amplification o ... | 2017 | 28889914 |
| vaccination against furunculosis in arctic char: efficacy of a commercial vaccine. | arctic char salvelinus alpinus are susceptible to furunculosis, a serious bacterial disease that affects the productivity of salmonid farms around the world. the objective of this laboratory study was to evaluate the efficacy of a commercially available vaccine against furunculosis in two strains of arctic char. labrador and nauyuk char were injected with aqua health furogen 2 or 0.9% nacl. at 87 and 108 d postvaccination, fish were challenged with aeromonas salmonicida by immersion. in both cha ... | 2002 | 28880796 |
| infections of edwardsiella tarda among brook trout in quebec. | acute bacterial septicemia is commonly diagnosed in brook trout salvelinus fontinalis of quebec, canada. the agents most commonly isolated include aeromonas salmonicida (furunculosis), aeromonas hydrophila (motile aeromonad septicemia), and pseudomonas species. septicemia in brook trout caused by the gram-negative bacterium edwardsiella tarda was diagnosed for the first time in the province of quebec from two different fish farms producing stock for fee fishing establishments. affected fish disp ... | 2000 | 28880779 |
| aldh7a1 is a protein that protects atlantic salmon against aeromonas salmonicida at the early stages of infection. | aldehyde dehydrogenases (aldhs) belong to a super-family of detoxifying proteins and perform a significant role in developing epithelial homeostasis, protecting cells from toxic aldehydes and drug resistance. however, the activity and function of these detoxifying proteins remain unknown, especially in fish. in our research, we aimed to study functions of aldehyde dehydrogenase 7a1 (aldh7a1) in atlantic salmon infected by aeromonas salmonicida. recombinant aldh7a1 (raldh7a1) was verified by sds- ... | 2017 | 28867386 |
| evaluation of antibody response to an adjuvanted hapten-protein vaccine as a potential inhibitor of sexual maturation for farmed atlantic salmon. | an experimental contraceptive vaccine was evaluated in atlantic salmon (salmo salar). a peptide derived from the beta subunit of luteinizing hormone (lh) was conjugated to two different carrier proteins, bovine serum albumin (bsa) and keyhole limpet hemocyanin (klh), and formulated with one of four immunostimulants in a water-in-oil emulsion. specific antibody responses to the peptide and each carrier protein were evaluated. while the antibody response to klh was stronger than the response to bs ... | 2017 | 28866277 |
| chromosome-mediated mcr-3 variants in aeromonas veronii from chicken meat. | two adjacent colistin resistance gene variants, termed mcr-3.2 and mcr-3-like, were identified in the chromosome of an aeromonas veronii isolated from retail chicken meat. the variants showed 95.20% and 84.19% nucleotide sequence identity, respectively, to mcr-3 from porcine escherichia coli functional cloning indicated that only mcr-3.2 conferred polymyxin resistance in both e. coli and aeromonas salmonicida the mcr-3.2-mcr-3-like segment was also observed in other aeromonas species, including ... | 2017 | 28848017 |
| characterization and diversity of phages infecting aeromonas salmonicida subsp. salmonicida. | phages infecting aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 a. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. this bacterial set als ... | 2017 | 28765570 |
| in vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens. | antibiotic resistance is a global issue that threatens public health. the excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. the aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major ... | 2017 | 28732514 |
| comparative pathogenicity of vibrio spp., photobacterium damselae ssp. damselae and five isolates of aeromonas salmonicida ssp. achromogenes in juvenile atlantic halibut (hippoglossus hippoglossus). | juvenile atlantic halibut (~100 mg, hippoglossus hippoglossus) were exposed to vibrio proteolyticus, a vibrio spp. isolate, photobacterium damselae ssp. damselae and five different isolates of aeromonas salmonicida ssp. achromogenes via an hour-long bath immersion to ascertain their variation in pathogenicity to this fish species. results were analysed using kaplan-meier survival analysis. analysis of the data from challenges using a. salmonicida ssp. achromogenes revealed three survival values ... | 2017 | 28731211 |
| alternatives to mineral oil adjuvants in vaccines against aeromonas salmonicida subsp. salmonicida in rainbow trout offer reductions in adverse effects. | in an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. using rainbow trout (oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated aeromonas salmonicida subspecies salmonicida were formulated with cpg oligodeoxynucleotides, the liposomal cationic adjuvant formulation 0 ... | 2017 | 28724973 |
| a historical review of the key bacterial and viral pathogens of scottish wild fish. | thousands of scottish wild fish were screened for pathogens by marine scotland science. a systematic review of published and unpublished data on six key pathogens (renibacterium salmoninarum, aeromonas salmonicida, ipnv, isav, sav and vhsv) found in scottish wild and farmed fish was undertaken. despite many reported cases in farmed fish, there was a limited number of positive samples from scottish wild fish, however, there was evidence for interactions between wild and farmed fish. a slightly el ... | 2017 | 28718925 |
| plasmid composition in aeromonas salmonicida subsp. salmonicida 01-b526 unravels unsuspected type three secretion system loss patterns. | aeromonas salmonicida subsp. salmonicida is a ubiquitous psychrophilic waterborne bacterium and a fish pathogen. the numerous mobile elements, especially insertion sequences (is), in its genome promote rearrangements that impact its phenotype. one of the main virulence factors of this bacterium, its type three secretion system (ttss), is affected by these rearrangements. in aeromonas salmonicida subsp. salmonicida most of the ttss genes are encoded in a single locus on a large plasmid called pas ... | 2017 | 28701230 |
| novel plasmid-mediated colistin resistance gene mcr-3 in escherichia coli. | the mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant gram-negative bacteria. to date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the genbank database. here, we characterized a third mobile colistin resistance gene, mcr-3 the gene coexisted ... | 2017 | 28655818 |
| analysis of adipose tissue immune gene expression after vaccination of rainbow trout with adjuvanted bacterins reveals an association with side effects. | most existing fish vaccines are presented in the form of oil-based emulsions delivered by intraperitoneal injection. whilst very effective they are frequently associated with inflammatory responses that can result in clinically significant side-effects often involving the adipose tissue that is in direct contact with the vaccine. to explore the potential of immune gene expression changes in the adipose tissue of fish to be markers of vaccination efficacy or development of side-effects we have st ... | 2017 | 28622574 |
| analysis of the genome and mobilome of a dissimilatory arsenate reducing aeromonas sp. o23a reveals multiple mechanisms for heavy metal resistance and metabolism. | aeromonas spp. are among the most ubiquitous microorganisms, as they have been isolated from different environmental niches including waters, soil, as well as wounds and digestive tracts of poikilothermic animals and humans. although much attention has been paid to the pathogenicity of aeromonads, the role of these bacteria in environmentally important processes, such as transformation of heavy metals, remains to be discovered. therefore, the aim of this study was a detailed genomic characteriza ... | 2017 | 28611742 |
| species distribution and prevalence of putative virulence factors in mesophilic aeromonas spp. isolated from fresh retail sushi. | aeromonas spp. are ubiquitous bacteria that have received increasing attention as human pathogens because of their widespread occurrence in food, especially seafood and vegetables. the aim of this work was to assess the species identity and phylogenetic relationship of 118 aeromonas strains isolated from fresh retail sushi from three producers, and to characterize the isolates with respect to genetic and phenotypic virulence factors. we also evaluate the potential hazard associated with their pr ... | 2017 | 28596762 |
| aeromonas salmonicida bacteremia associated with chronic well water consumption in a patient with diabetes. | aeromonas salmonicida is associated with superficial skin infections in fish. its virulence factors allow colonization of water including surface water such as salt water, beaches, and fresh water wells. moreover, it is possible for immunocompromised patients to develop invasive disease after chronic exposure to aeromonas spp. through contaminated water. while there are reports of aeromonas spp. bacteremia following water ingestion, there have been no reports of a. salmonicida bacteremia from wa ... | 2017 | 28584462 |
| characterization of the cultivable gut microflora in wild-caught mediterranean fish species. | microflora of the gastrointestinal tract plays important roles in food digestion, nutrient absorption and in host defense against ingested pathogens. several studies have focused on the microflora of farmed fishes, but the gut flora of wild fishes remains poorly characterized. the aim of this work was to provide an overview of the bacteria colonizing the gut of wild-caught fishes and to determine whether some bacterial species can be pathogenic. | 2017 | 28553195 |
| phenotype of aeromonas salmonicida sp. salmonicida cyclic adenosine 3',5'-monophosphate receptor protein (crp) mutants and its virulence in rainbow trout (oncorhynchus mykiss). | precise deletion of genes related to virulence can be used as a strategy to produce attenuated bacterial vaccines. here, we study the deletion of the cyclic-3',5'-adenosine monophosphate (camp) receptor protein (crp) in aeromonas salmonicida, the aetiologic agent of furunculosis in marine and freshwater fish. the crp protein is a conserved global regulator, controlling physiology processes, like sugar utilization. deletion of the crp gene has been utilized in live attenuated vaccines for mammals ... | 2017 | 28548689 |
| studies on the antibody response and side effects after intramuscular and intraperitoneal injection of atlantic lumpfish (cyclopterus lumpus l.) with different oil-based vaccines. | atlantic lumpfish (cyclopterus lumpus l.) is used as a biological delousing agent for sea lice (lepeophtheirus salmonis k.) infestations in norwegian aquaculture. here, we present a study on the antibody response and vaccine side effects after intramuscular and intraperitoneal injection of lumpfish with two vaccines. both vaccines contained bacterial antigens from atypical aeromonas salmonicida a-layer types v and vi, vibrio anguillarum serotype o1 and moritella viscosa sp., but one vaccine cont ... | 2017 | 28548686 |
| aeromonas salmonicida growth in response to atlantic salmon mucins differs between epithelial sites, is governed by sialylated and n-acetylhexosamine-containing o-glycans, and is affected by ca(2). | aeromonas salmonicida causes furunculosis in salmonids and is a threat to atlantic salmon aquaculture. the epithelial surfaces that the pathogen colonizes are covered by a mucus layer predominantly comprised of secreted mucins. by using mass spectrometry to identify mucin glycan structures with and without enzymatic removal of glycan residues, coupled to measurements of bacterial growth, we show here that the complex atlantic salmon intestinal mucin glycans enhance a. salmonicida growth, whereas ... | 2017 | 28533470 |
| in vitro effects of italian lavandula multifida l. leaf extracts on gilthead seabream (sparus aurata) leucocytes and saf-1 cells. | lavandula multifida is very appreciated by pharmaceutical and cosmetic industries. in italy is only found in calabria and sicily and, at present, urge its valorization due to its high extinction and genetic erosion risks. possible applications of l. multifida extracts as immunostimulant in fish aquaculture were assayed by using gilthead seabream (sparus aurata) as a marine fish model, due to its importance in fish aquaculture. the in vitro effects of both aqueous and ethanolic leaf extracts obta ... | 2017 | 28522420 |
| differential effects of exposure to parasites and bacteria on stress response in turbot scophthalmus maximus simultaneously stressed by low water depth. | the stress response of turbot scophthalmus maximus was evaluated in fish maintained 8 days under different water depths, normal (nwd, 30 cm depth, total water volume 40 l) or low (lwd, 5 cm depth, total water volume 10 l), in the additional presence of infection-infestation of two pathogens of this species. this was caused by intraperitoneal injection of sublethal doses of the bacterium aeromonas salmonicida subsp. salmonicida or the parasite philasterides dicentrarchi (ciliophora:scuticociliati ... | 2017 | 28516502 |
| the protective effect of humic-rich substances on atypical aeromonas salmonicida subsp. salmonicida infection in common carp (cyprinus carpio l.). | when challenged with atypical aeromonas salmonicida subsp. salmonicida, exposure of the common carp (cyprinus carpio l.) to different humic-rich compounds resulted in a significant reduction in infection rates. specifically, in fish exposed to (i) humic-rich water and sludge from a recirculating system, (ii) a synthetic humic acid, and (iii) a leonardite-derived humic-rich extract, infection rates were reduced to 14.9%, 17.0% and 18.8%, respectively, as compared to a 46.8% infection rate in the ... | 2017 | 28493490 |
| the biofilteration ability of oysters (crassostrea gigas) to reduce aeromonas salmonicida in salmon culture. | pathogen contamination in the environment is inevitable with the rapid development of intensive aquaculture. therefore, alternative ecofriendly biological strategies to control pathogenic bacteria are required. however, our aim was to investigate the ability of oysters (crassostrea gigas) to filter the important opportunistic pathogen, aeromonas salmonicida (strain c4), using a green fluorescent protein tag (gfp) in the atlantic salmon (salmo salar) farming wastewater. hence, a. salmonicida remo ... | 2017 | 28474104 |
| transcriptional changes in innate immunity genes in head kidneys from aeromonas salmonicida-challenged rainbow trout fed a mixture of polycyclic aromatic hydrocarbons. | we previously observed that exposure to a complex mixture of high molecular weight polycyclic aromatic hydrocarbons (pahs) increased sensitivity of rainbow trout (oncorhynchus mykiss) to subsequent challenge with aeromonas salmonicida, the causative agent of furunculosis. in this study, we evaluate potential mechanisms associated with disease susceptibility from combined environmental factors of dietary pah exposure and pathogen challenge. rainbow trout were fed a mixture of ten high molecular w ... | 2017 | 28407501 |
| nocardiosis in freshwater reared chinook salmon (oncorhynchus tshawytscha). | an investigation was conducted to identify the cause of mortalities in freshwater reared chinook salmon (oncorhynchus tshawytscha). mortalities occurred in juvenile salmon, at a salmon rearing facility in the south island of new zealand. the affected fish were from a pen inside the facility with no surrounding pens or other year classes affected. | 2017 | 28372487 |
| echinoderm immunology: bacterial clearance by the sea urchin strongylocentrotus purpuratus. | characteristics of bacterial clearance were investigated in the purple sea urchin, strongylocentrotus purpuratus (echinodermata: echinoidea). primary clearance kinetics were determined for three bacteria, a marine gram negative motile rod, a marine gram positive non-motile rod, and a gram negative freshwater fish pathogen, aeromonas salmonicida. clearance kinetics differed for each of the three bacteria. secondary clearance rates were not significantly different from primary clearance rates for ... | 1983 | 28368229 |
| antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago. | twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ... | 2016 | 28352539 |
| protection and antibody reactivity following vaccination of lumpfish (cyclopterus lumpus l.) against atypical aeromonas salmonicida. | atypical aeromonas salmonicida is frequently associated with disease and mortality in farmed lumpfish (cyclopterus lumpus l). challenge experiments using different modes of exposure identified both high and low pathogenic isolates. intraperitoneal vaccination induced production of high levels of specific antibodies particularly in fish given multiple injections. the immune sera contained antibodies cross reactive with both high and low pathogenic isolates. sds-page and lc/msms analyses showed th ... | 2017 | 28344167 |
| antimicrobial effect of the biotronic® top3 supplement and efficacy in protecting rainbow trout (oncorhynchus mykiss) from infection by aeromonas salmonicida subsp. salmonicida. | demand for more environmentally friendly practices have led to the adoption of several feed supplements by the fish farming industry. in the present study, we investigated a commercially available formula that includes a mixture of three compounds: organic acids, a phytochemical and biomin® permeabilizing complex. this mixture demonstrated antimicrobial properties in vitro and was able to inhibit growth of multiple species of aquatic bacterial pathogens, including aeromonas salmonicida. bacteria ... | 2017 | 28343146 |
| phylogeny and expression analysis of c-reactive protein (crp) and serum amyloid-p (sap) like genes reveal two distinct groups in fish. | the acute phase response (apr) is an early innate immune function that is initiated by inflammatory signals, leading to the release of acute phase proteins to the bloodstream to re-establish homeostasis following microbial infection. in this study we analysed the atlantic salmon (salmo salar) whole-genome database and identified five c-reactive protein (crp)/serum amyloid p component (sap) like molecules namely crp/sap-1a, crp/sap-1b, crp/sap-1c, crp/sap-2 and crp/sap-3. these crp/sap genes form ... | 2017 | 28336487 |
| effect of dietary β-glucan on growth, survival and regulation of immune processes in rainbow trout (oncorhynchus mykiss) infected by aeromonas salmonicida. | the present study evaluated the effects of dietary β-glucan (0, 0.05%, 0.1%, and 0.2%) on growth performance after 42 days of feeding. thereafter, rainbow trout (oncorhynchus mykiss) were infected with aeromonas salmonicida, and survival rates as well as the regulating processes of stress- and immune-related factors were analyzed. in general, higher dietary β-glucan levels obviously improved specific growth rate (sgr), weight gain (wg) and feed efficiency (fe) (p ≤ 0.05). survival rates in β-glu ... | 2017 | 28286256 |
| biodegradation of benzo[a]pyrene by bacterial consortium isolated from mangrove sediment. | benzo[a]pyrene is a high-molecular-weight polycyclic aromatic hydrocarbon highly recalcitrant in nature and thus harms the ecosystem and/or human health. therefore, its removal from the marine environment is crucial. this research focuses on benzo[a]pyrene degradation by using enriched bacterial isolates in consortium under saline conditions. bacterial isolates capable of using benzo[a]pyrene as sole source of carbon and energy were isolated from enriched mangrove sediment. these isolates were i ... | 2017 | 28281885 |
| involvement of luxs in aeromonas salmonicida metabolism, virulence and infection in atlantic salmon (salmo salar l). | quorum sensing is a bacterial density dependent communication system, which regarded to regulate co-operative behaviors of community and mediated by extracellular signal molecules named autoinducers (ai). among various signals, autoinducer-2 (ai-2) is believed to be the messengers inter species and produced by luxs. for aeromonas salmonicida (a. salmonicida), an opportunistic pathogen to many cold-water teleost, little information has been known about the function of ai-2 and luxs. therefore, ou ... | 2017 | 28279794 |
| draft genome sequence of pseudomonas fluorescens ml11a, an endogenous strain from brook charr with antagonistic properties against aeromonas salmonicida subsp. salmonicida. | pseudomonas fluorescens ml11a, isolated from brook charr, showed a strong in vitro inhibitory effect against aeromonas salmonicida subsp. salmonicida, a bacterial fish pathogen. its genome harbors gene clusters for siderophore and bacteriocin biosynthesis and shares 99% whole-genome identity with p. fluorescens a506, a biological control strain used in agriculture. | 2017 | 28254983 |
| proteomic analysis in kidneys of atlantic salmon infected with aeromonas salmonicida by itraq. | aeromonas salmonicida is a major etiologic agent which induces furunculosis and is globally harmful in salmonid and turbot cultures, especially in atlantic salmon (salmo salar) farming. in order to improve knowledge of its poorly understood pathogenesis, we utilized high-throughput proteomics to display differentially expressed proteins in the kidney of atlantic salmon challenged with high and low infection dose of a. salmonicida at 7 and 14 days. in quantitative proteomic assays, isobaric tags ... | 2017 | 28235584 |
| subunit vaccine candidates against aeromonas salmonicida in rainbow trout oncorhynchus mykiss. | aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis and a major fish health problem in salmonid aquaculture worldwide. injection vaccination with commercial mineral oil-adjuvanted bacterin vaccines has been partly successful in preventing the disease but in danish rainbow trout (oncorhynchus mykiss, walbaum) aquaculture furunculosis outbreaks still occur. in this study we tested the efficacy of experimental subunit vaccines against a. salmonicida infection in rainbo ... | 2017 | 28182704 |
| phylogenetic analysis of the fish pathogen aeromonas salmonicida underlines the dichotomy between european and canadian strains for the salmonicida subspecies. | 2017 | 28105757 | |
| characterization and mechanism of anti-aeromonas salmonicida activity of a marine probiotic strain, bacillus velezensis v4. | the bacterium aeromonas salmonicida is the causative agent of furunculosis, a systemic, ubiquitous disease of fish in the salmon family, characterized by high mortality and morbidity. probiotics are a promising approach for prevention of furunculosis in aquaculture. a bacterial strain with anti-a. salmonicida properties, bacillus velezensis v4, was isolated and the mechanisms underlying these properties were investigated. anti-a. salmonicida compounds present in cell-free supernatant of v4 were ... | 2017 | 28074223 |
| use of common carp (cyprinus carpio) and aeromonas salmonicida for detection of immunomodulatory effects of chemicals on fish. | to develop a test for assessing the immunomodulatory effects of chemical pollutants on fish, we evaluated the effects of dexamethasone on the natural host-pathogen interaction between common carp (cyprinus carpio) and aeromonas salmonicida. carp were exposed to 1mgl(-1) dexamethasone for the entire experimental period. one week after the exposure test started, the exposed fish, as well as unexposed fish, were bath-infected with a. salmonicida. one hundred percent mortality was observed in bacter ... | 2017 | 28063699 |
| evaluation of the spoilage potential of bacteria isolated from chilled chicken in vitro and in situ. | microorganisms play an important role in the spoilage of chilled chicken. in this study, a total of 53 isolates, belonging to 7 species of 3 genera, were isolated using a selective medium based on the capacity to spoil chicken juice. four isolates, namely aeromonas salmonicida 35, pseudomonas fluorescens h5, pseudomonas fragi h8 and serratia liquefaciens 17, were further characterized to assess their proteolytic activities in vitro using meat protein extracts and to evaluate their spoilage poten ... | 2017 | 28040161 |
| identification of the ferric-acinetobactin outer membrane receptor in aeromonas salmonicida subsp. salmonicida and structure-activity relationships of synthetic acinetobactin analogues. | aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in several fish species, produces acinetobactin and amonabactin as siderophores. in a previous study, we chemically characterized these siderophores and proposed a biosynthetic pathway based on genetic analysis. however, the internalization mechanisms of ferric-acinetobactin and ferric-amonabactin remain largely unknown. in the present study, we demonstrate that the outer membrane protein fstb is the ferric-acinetobact ... | 2017 | 27936588 |
| aeromonas salmonicida type iii secretion system-effectors-mediated immune suppression in rainbow trout (oncorhynchus mykiss). | aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen in aquaculture. together with other pathogens, it is characterized by the presence of a type 3 secretion system (t3ss). the t3ss is the main virulence mechanism of a. salmonicida. it is used by the bacterium to secrete and translocate several toxins and effector proteins into the host cell. some of these factors have a detrimental impact on the integrity of the cell cytoskeleton, likely contributin ... | 2017 | 27923746 |
| development of a sybr green i real-time pcr assay for specific identification of the fish pathogen aeromonas salmonicida subspecies salmonicida. | a sybr green i real-time polymerase chain reaction protocol for specific detection of the fish pathogen aeromonas salmonicida subsp. salmonicida was developed and validated for rapid diagnosis of typical furunculosis. the sequence of the aopo gene of a. salmonicida subsp. salmonicida, which encodes for a serine/threonine protein kinase linked to virulence, was chosen for primer design. the selected primers amplified a 119-bp internal fragment of the aopo gene. the specificity test proved that 10 ... | 2016 | 27838837 |
| multiple gene and transcript variants encoding trout c-polysaccharide binding proteins are differentially but strongly induced after infection with aeromonas salmonicida. | two 'trout c-polysaccharide-binding proteins,' tcbp1 and -2, with relevance to early inflammatory events have been discovered in the last century. the present study characterises the respective cdna sequences from rainbow trout (oncorhynchus mykiss), including multiple tcbp1 transcript variants. these variants are generated either by the use of alternative splice sites or the exclusion of exons. the longest mrna isoform, tcbp1-1, encodes a 245-aa protein with a large signal peptide and a complem ... | 2017 | 27836722 |
| preliminary study on the in vitro and in vivo effects of asparagopsis taxiformis bioactive phycoderivates on teleosts. | several compounds from marine organisms have been studied for their potential use in aquaculture. among the red algae, asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. depending on the seasonal period of sampling, ethanol extracts of a. taxiformis exhibited signi ... | 2016 | 27826246 |
| the mosaic architecture of aeromonas salmonicida subsp. salmonicida pasa4 plasmid and its consequences on antibiotic resistance. | aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in salmonids, is an issue especially because many isolates of this bacterium display antibiotic resistances, which limit treatments against the disease. recent results suggested the possible existence of alternative forms of pasa4, a large plasmid found in a. salmonicida subsp. salmonicida and bearing multiple antibiotic resistance genes. the present study reveals the existence of two newly detected pasa4 variants, pas ... | 2016 | 27812409 |
| st2 from rainbow trout quenches tlr signalling, localises at the nuclear membrane and allows the nuclear translocation of myd88. | the mammalian interleukin 1 receptor-like 1 receptor (il1rl1), commonly known as st2, is thought to downregulate tlr signalling by sequestering the signalling adapter myd88 (myeloid differentiation primary response protein 88). st2 sequences are known in several fish species, but none of them have functionally been examined. we characterised st2 from rainbow trout (oncorhynchus mykiss) and the structure of its encoding gene. the primary sequence of st2 is only weakly conserved from fish to human ... | 2017 | 27776995 |
| diversity of antibiotic-resistance genes in canadian isolates of aeromonas salmonicida subsp. salmonicida: dominance of psn254b and discovery of pasa8. | the bacterium aeromonas salmonicida subsp. salmonicida is a common pathogen in fish farms worldwide. since the antibiotic resistance of this bacterial species is on the increase, it is important to have a broader view on this issue. in the present study, we tested the presence of known plasmids conferring multi-drug resistance as well as antibiotic resistance genes by a pcr approach in 100 canadian a. salmonicida subsp. salmonicida isolates. our study highlighted the dominance of the conjugative ... | 2016 | 27752114 |
| the effects of increasing dietary levels of soy protein concentrate (spc) on the immune responses and disease resistance (furunculosis) of vaccinated and non-vaccinated atlantic salmon (salmo salar l.) parr. | juvenile salmon, with an initial weight of 9 g, were fed three experimental diets, formulated to replace 35 (spc35), 58 (spc58) and 80 (spc80) of high quality fishmeal (fm) with soy protein concentrate (spc) in quadruplicate tanks. higher dietary spc inclusion was combined with increased supplementation of methionine, lysine, threonine and phosphorus. the experiment was carried out for 177 days. on day 92 salmon in each tank were bulk weighed. post weighing eighty salmon from each tank were redi ... | 2016 | 27742588 |
| pathogen-dependent role of turbot (scophthalmus maximus) interferon-gamma. | interferon-gamma has been typically described as a pro-inflammatory cytokine playing an important role in the resolution of both viral and bacterial diseases. nevertheless, some anti-inflammatory functions are also attributed to this molecule. in this work we have characterized for the first time the turbot (scophthalmus maximus) interferon-gamma gene (ifng) and its expression pattern under basal conditions, after type i ifns administration, and viral and bacterial infection. the intramuscular i ... | 2016 | 27742586 |
| positive correlation between aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout oncorhynchus mykiss evaluated by a tail fin infection model. | rainbow trout, oncorhynchus mykiss (walbaum), are able to raise a protective immune response against aeromonas salmonicida subsp. salmonicida (as) following injection vaccination with commercial vaccines containing formalin-killed bacteria, but the protection is often suboptimal under danish mariculture conditions. we elucidated whether protection can be improved by increasing the concentration of antigen (formalin-killed bacteria) in the vaccine. rainbow trout juveniles were vaccinated by intra ... | 2017 | 27593619 |
| assessment of hemolytic activity, enzyme production and bacteriocin characterization of bacillus subtilis lr1 isolated from the gastrointestinal tract of fish. | in the present investigation, probiotic potential (antagonistic activity, enzyme production, hemolytic activity, biosafety, antibiotic sensitivity and bile tolerance level) of bacillus subtilis lr1 was evaluated. bacteriocin produced by the bacterial strain b. subtilis lr1 isolated from the gastrointestinal tract of labeo rohita was purified and characterized. the molecular weight of the purified bacteriocin was ~50 kda in 12 % native page and showed inhibitory activity against four fish pathoge ... | 2017 | 27590016 |
| metabolic profiling in kidneys of atlantic salmon infected with aeromonas salmonicida based on (1)h nmr. | aeromonas salmonicida, an important pathogenic bacterium which induces furunculosis, is globally causing increased risks in atlantic salmon (salmo salar) farming. although the kidney is the main target organ of a. salmonicida, the metabolic profiling of kidney in response to a. salmonicida in vivo remains unknown. here, we used (1)h nuclear magnetic resonance (nmr) to comprehensively analyze the metabolic changes in the kidney of atlantic salmon. through the noesypr1d spectrum combined with mult ... | 2016 | 27577538 |
| a comparison of the response of diploid and triploid atlantic salmon (salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with aeromonas salmonicida. | sterile triploid fish represent a solution to the problems associated with sexual maturation and escapees in aquaculture. however, as disease outbreaks continue to cause significant economic losses to the industry, it is essential that the response of triploids to disease and disease treatments be characterised. the aim of this study was to compare the response of triploid atlantic salmon to a commercial furunculosis vaccine with that of diploid fish, and to assess the vaccine efficacy in the tw ... | 2016 | 27569980 |
| probiotic potential of autochthonous bacteria isolated from the gastrointestinal tract of four freshwater teleosts. | in this study, a total of 121 bacterial strains were isolated from the gastrointestinal tract of four teleostean species, namely striped snakehead (channa striatus), striped dwarf catfish (mystus vittatus), orangefin labeo (labeo calbasu) and mrigal carp (cirrhinus mrigala), among which 8 isolates showed promising antibacterial activity against four potential fish pathogens, aeromonas hydrophila, aeromonas salmonicida, aeromonas sobria and pseudomonas fluorescens and were non-hemolytic. the isol ... | 2017 | 27557836 |
| structural evidence of intramolecular propeptide inhibition of the aspzincin metalloendopeptidase asap1. | the gram-negative bacterium aeromonas salmonicida is a fish pathogen for various fish species worldwide. aeromonas salmonicida subsp. achromogenes produces the extracellular, toxic zinc endopeptidase asap1. crystal structure analyses at 2.0 å resolution of two proteolytically inactive asap1 variants show the polypeptide folding of the protease domain and the propeptide domain. these first crystal structure analyses of a precursor of a deuterolysin-like aspzincin protease provide insights into pr ... | 2016 | 27528449 |
| the flgt protein is involved in aeromonas hydrophila polar flagella stability and not affects anchorage of lateral flagella. | aeromonas hydrophila sodium-driven polar flagellum has a complex stator-motor. consist of two sets of redundant and non-exchangeable proteins (poma/pomb and poma2/pomb2), which are homologs to other sodium-conducting polar flagellum stator motors; and also two essential proteins (motx and moty), that they interact with one of those two redundant pairs of proteins and form the t-ring. in this work, we described an essential protein for polar flagellum stability and rotation which is orthologs to ... | 2016 | 27507965 |
| pharmacokinetic model of florfenicol in turbot (scophthalmus maximus): establishment of optimal dosage and administration in medicated feed. | the pharmacokinetics of florfenicol (ff) in turbot (scophthalmus maximus) was studied after single intravenous (10 mg kg(-1) ) and oral (100 mg kg(-1) ) administration. the plasma concentration-time data of florfenicol were described by an open one-compartment model. the elimination half-life (t1/2 ) was estimated to be 21.0 h, and the total body clearance, cl, was determined as 0.028 l kg h(-1) . the apparent volume distribution (vd ) was calculated to be 0.86 l kg(-1) and the mean residence ti ... | 2017 | 27502011 |
| aeromonas salmonicida subsp. salmonicida strains isolated from chinese freshwater fish contain a novel genomic island and possible regional-specific mobile genetic elements profiles. | two strains of aeromonas salmonicida, yk and bg, were isolated from largemouth bronze gudgeon and northern whitefish in china, and identified as a. salmonicida subsp. salmonicida based on phylogenetic analysis of vapa and 16s rrna gene sequences. yk and bg originated from freshwater fish, one of which belonged to the cyprinid family, and the strains showed a difference in virulence. subsequently, we performed whole genome sequencing of the strains, and comparison of their genomic sequences to th ... | 2016 | 27493011 |
| alterations of the gut microbiome of largemouth bronze gudgeon (coreius guichenoti) suffering from furunculosis. | high-throughput sequencing was applied to compare the intestinal microbiota in largemouth bronze gudgeon either healthy or affected by furunculosis. proteobacteria, actinobacteria, tenericutes, firmicutes and bacteroidetes were detected as the predominant bacterial phyla in the gut of both diseased and healthy fish. the abundance of proteobacteria differed significantly between the two groups of fish, mainly due to the overwhelming prevalence of aeromonas in the diseased fish (81% ± 17%), while ... | 2016 | 27465687 |
| antimicrobial activities of bacteria associated with the brown alga padina pavonica. | macroalgae belonging to the genus padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. here we report antibacterial activities of epiphytic bacteria isolated from padina pavonica (peacocks tail) located on northern coast of tunisia. eighteen isolates were obtained in pure culture and tested ... | 2016 | 27462308 |
| immunohistochemical study of inducible nitric oxide synthase and tumour necrosis factor alpha response in turbot (scophthalmus maximus) experimentally infected with aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida represents one of the major threats in aquaculture, especially in salmonid fish and turbot farming. in order to fight bacterial infections, fish have an immune system composed by innate and specific cellular and humoral elements analogous to those present in mammals. however, innate immunity plays a primordial role against bacterial infections in teleost fish. among these non-specific mechanisms, the production of nitric oxide (no) by inducible nitric oxi ... | 2016 | 27431586 |
| evaluation of different conditions and culture media for the recovery of aeromonas spp. from water and shellfish samples. | to perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ada), starch ampicillin agar (saa), bile salts irgasan brilliant green modified (bibg-m)) for recovering aeromonas species from water and shellfish samples. | 2016 | 27333573 |
| infection routes of aeromonas salmonicida in rainbow trout monitored in vivo by real-time bioluminescence imaging. | recent development of imaging tools has facilitated studies of pathogen infections in vivo in real time. this trend can be exemplified by advances in bioluminescence imaging (bli), an approach that helps to visualize dissemination of pathogens within the same animal over several time points. here, we employ bacterial bli for examining routes of entry and spread of aeromonas salmonicida susbp. salmonicida in rainbow trout. a virulent danish a. salmonicida strain was tagged with pakgfplux1, a dual ... | 2017 | 27307098 |
| aeromonas salmonicida: updates on an old acquaintance. | aeromonas salmonicida is the oldest known infectious agent to be linked to fish disease and constitutes a major bacterial pathogen of fish, in particular of salmonids. this bacterium can be found almost worldwide in both marine and freshwater environments and has been divided into several sub-species. in this review, we present the most recent developments concerning our understanding of this pathogen, including how the characterization of new isolates from non-salmonid hosts suggests a more nua ... | 2016 | 27304870 |
| the effect of reducing agents on challenge of rainbow trout with aeromonas salmonicida. | 2017 | 27291986 | |
| expression and antimicrobial activity of c-type lysozyme in taimen (hucho taimen, pallas). | lysozymes are important defense proteins of the innate immune system and possess high antibacterial activities. in the present study, a full-length c-type lysozyme cdna (htlysc) was cloned and characterized from taimen (hucho taimen, pallas). the cdna contains an open reading frame (orf) of 432 bp encoding 143 amino acid (aa), with 97% identity to lysc of rainbow trout (oncorhynchus mykiss). the amino acid sequence possessed a lyz1 domain (16-140 aa) which contained two conserved residues (glu 5 ... | 2016 | 27267655 |
| detection and quantification of aeromonas salmonicida in fish tissue by real-time pcr. | furunculosis, a septicaemic infection caused by the bacterium aeromonas salmonicida subsp. salmonicida, currently causes problems in danish seawater rainbow trout production. detection has mainly been achieved by bacterial culture, but more rapid and sensitive methods are needed. a previously developed real-time pcr assay targeting the plasmid encoded aopp gene of a. salmonicida was, in parallel with culturing, used for the examination of five organs of 40 fish from danish freshwater and seawate ... | 2017 | 27193829 |
| toll-like receptors in maraena whitefish: evolutionary relationship among salmonid fishes and patterns of response to aeromonas salmonicida. | toll-like receptors (tlrs) interact directly with particular pathogenic structures and are thus highly important to innate immunity. the present manuscript characterises a suite of 14 tlrs in maraena whitefish (coregonus maraena), a salmonid species with increasing importance for aquaculture. whitefish tlrs were structurally and evolutionary analysed. the results revealed a close relationship with tlrs from salmonid fish species rainbow trout and atlantic salmon. profiling the baseline expressio ... | 2016 | 27131902 |
| cold-water vibriosis. the current status of knowledge. | the current review for the first time summarizes the findings of the 30 years of research on cold-water vibriosis (cwv). the diseased caused by aliivibrio salmonicida (earlier known as vibrio salmonicida) was for the first time described in 1986 and became one of the most important bacterial diseases in salmon aquaculture. the lack of appropriate vaccine hampered development of atlantic salmon aquaculture until the late 1980s when a novel vaccine allowed dramatic increase in the atlantic salmon ... | 2017 | 27072873 |
| cross talk between growth and immunity: coupling of the igf axis to conserved cytokine pathways in rainbow trout. | although disease and infection is associated with attenuated growth, the molecular pathways involved are poorly characterized. we postulated that the igf axis, a central governor of vertebrate growth, is repressed during infection to promote resource reallocation towards immunity. this hypothesis was tested in rainbow trout (oncorhynchus mykiss) challenged by aeromonas salmonicida (as), a gram-negative bacterial pathogen, or viral hemorrhagic septicemia virus (vhsv) at hatch, first feeding, and ... | 2016 | 27035654 |
| antibiotic resistance due to an unusual cole1-type replicon plasmid in aeromonas salmonicida. | aeromonas salmonicida subsp. salmonicida is a fish pathogen known to have a rich plasmidome. in the present study, we discovered an isolate of this bacterium bearing an additional unidentified small plasmid. after having sequenced the dna of that isolate by next-generation sequencing, it appeared that the new small plasmid is a cole1-type replicon plasmid, named here pasa7. this plasmid bears a functional chloramphenicol-acetyltransferase-encoding gene (cat-pasa7) previously unknown in a. salmon ... | 2016 | 27028891 |
| facile recoding of selenocysteine in nature. | selenocysteine (sec or u) is encoded by uga, a stop codon reassigned by a sec-specific elongation factor and a distinctive rna structure. to discover possible code variations in extant organisms we analyzed 6.4 trillion base pairs of metagenomic sequences and 24 903 microbial genomes for trna(sec) species. as expected, uga is the predominant sec codon in use. we also found trna(sec) species that recognize the stop codons uag and uaa, and ten sense codons. selenoprotein synthesis programmed by ua ... | 2016 | 26991476 |
| development of similar broth microdilution methods to determine the antimicrobial susceptibility of flavobacterium columnare and f. psychrophilum. | flavobacterium columnare and f. psychrophilum are major fish pathogens that cause diseases that may require antimicrobial therapy. choice of appropriate treatment is dependent upon determining the antimicrobial susceptibility of isolates. therefore we optimized methods for broth microdilution testing of f. columnare and f. psychrophilum to facilitate standardizing an antimicrobial susceptibility test. we developed adaptations to make reproducible broth inoculums and confirmed the proper incubati ... | 2016 | 26949840 |
| the aeromonas salmonicida lipopolysaccharide core from different subspecies: the unusual subsp. pectinolytica. | initial hydridization tests using aeromonas salmonicida typical and atypical strains showed the possibility of different lipopolysaccharide (lps) outer cores among these strains. by chemical structural analysis, lps-core sds-page gel migration, and functional and comparative genomics we demonstrated that typical a. salmonicida (subsp. salmonicida) strains and atypical subsp. masoucida and probably smithia strains showed the same lps outer core. a. salmonicida subsp. achromogenes strains show a s ... | 2016 | 26904002 |
| investigations on the role of the salmon louse, lepeophtheirus salmonis (caligidae), as a vector in the transmission of aeromonas salmonicida subsp. salmonicida. | a bacteria-parasite challenge model was used to study the role of sea lice, lepeophtheirus salmonis (copepoda), as a vector of aeromonas salmonicida subsp. salmonicida. three hypotheses were tested: (i) l. salmonis can acquire a. salmonicida subsp. salmonicida via water bath exposure; (ii) l. salmonis can acquire the bacteria via parasitizing infected atlantic salmon, salmo salar; and (iii) l. salmonis can transmit the bacteria to naïve atlantic salmon via parasitism. adult l. salmonis exposed t ... | 2016 | 26851068 |
| challenge models for atypical aeromonas salmonicida and vibrio anguillarum in farmed ballan wrasse (labrus bergylta) and preliminary testing of a trial vaccine against atypical aeromonas salmonicida. | 2016 | 26849912 | |
| characterization of gp21 and gp12: two potential probiotic bacteria isolated from the gastrointestinal tract of atlantic cod. | this study evaluated the probiotic potential of gp21 (pseudomonas sp.) and gp12 (psychrobacter sp.), two bacteria isolated from the intestinal tract of a cold-water fish, atlantic cod. the antagonistic activity of the two intestinal bacteria against two fish pathogens (vibrio anguillarum and aeromonas salmonicida subsp. salmonicida) was studied under different physical conditions. further, their resistance to physiological barriers and their ability to form biofilms were examined. in addition, a ... | 2010 | 26781122 |
| increasing genomic diversity and evidence of constrained lifestyle evolution due to insertion sequences in aeromonas salmonicida. | aeromonads make up a group of gram-negative bacteria that includes human and fish pathogens. the aeromonas salmonicida species has the peculiarity of including five known subspecies. however, few studies of the genomes of a. salmonicida subspecies have been reported to date. | 2016 | 26753691 |
| bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology. | bacteriophages have been proposed as an alternative to antibiotic usage and several studies on their application in aquaculture have been reported. this review highlights progress to date on phage therapies for the following fish and shellfish diseases and associated pathogens: hemorrhagic septicemia (aeromonas hydrophila) in loaches, furunculosis (aeromonas salmonicida) in trout and salmon, edwardsiellosis (edwardsiella tarda) in eel, columnaris disease (flavobacterium columnare) in catfish, ra ... | 2017 | 26713223 |
| effect of nanophyetus salmincola and bacterial co-infection on mortality of juvenile chinook salmon. | the freshwater trematode nanophyetus salmincola has been demonstrated to impair salmonid immune function and resistance to the marine pathogen vibrio anguillarum, potentially resulting in ocean mortality. we examined whether infection by the parasite n. salmincola similarly increases mortality of juvenile chinook salmon oncorhynchus tshawytscha when they are exposed to the freshwater pathogens flavobacterium columnare or aeromonas salmonicida, two bacteria that juvenile salmonids might encounter ... | 2015 | 26671546 |
| antigens of aeromonas salmonicida subsp. salmonicida specifically induced in vivo in oncorhynchus mykiss. | 2016 | 26661507 | |
| comparative evaluation of the antimicrobial activity of different antimicrobial peptides against a range of pathogenic bacteria. | the rapid emergence of resistance to classical antibiotics has increased the interest in novel antimicrobial compounds. antimicrobial peptides (amps) represent an attractive alternative to classical antibiotics and a number of different studies have reported antimicrobial activity data of various amps, but there is only limited comparative data available. the mode of action for many amps is largely unknown even though several models have suggested that the lipopolysaccharides (lps) play a crucia ... | 2015 | 26656394 |
| diversity and homogeneity among small plasmids of aeromonas salmonicida subsp. salmonicida linked with geographical origin. | furunculosis, which is caused by aeromonas salmonicida subsp. salmonicida, is a major salmonid disease in fish farms worldwide. several plasmids found in this bacterium confer phenotypes such drug resistance and virulence. small plasmids (pasa1, pasa2, pasa3, and pasal1) related to cole1- and cole2-type replicons are usually present in its normal plasmidome. in the present study, with the objective to investigate if these plasmids display particularities related to the origin of the isolates bea ... | 2015 | 26635745 |
| vaccination against furunculosis in arctic char: efficacy of a commercial vaccine. | arctic char salvelinus alpinus are susceptible to furunculosis, a serious bacterial disease that affects the productivity of salmonid farms around the world. the objective of this laboratory study was to evaluate the efficacy of a commercially available vaccine against furunculosis in two strains of arctic char. labrador and nauyuk char were injected with aqua health furogen 2 or 0.9% nacl. at 87 and 108 d postvaccination, fish were challenged with aeromonas salmonicida by immersion. in both cha ... | 2006 | 26599045 |