Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antimicrobial resistance in bacterial poultry pathogens: a review. | antimicrobial resistance (amr) is a global health threat, and antimicrobial usage and amr in animal production is one of its contributing sources. poultry is one of the most widespread types of meat consumed worldwide. poultry flocks are often raised under intensive conditions using large amounts of antimicrobials to prevent and to treat disease, as well as for growth promotion. antimicrobial resistant poultry pathogens may result in treatment failure, leading to economic losses, but also be a s ... | 2017 | 28848739 |
| genetic analysis of salmonella enterica serovar gallinarum biovar pullorum based on characterization and evolution of crispr sequence. | salmonella enterica serovar gallinarum biovar pullorum (s. pullorum) is the cause of pullorum disease, characterized by white diarrhea, which leads to high mortality in poultry. in this study, we aimed to assess the genetic diversity of 655 s. pullorum strains from 1962 to 2015 in china, europe, and south america. a sequence typing scheme based on clustered regularly interspaced short palindromic repeats (crispr) was used to reveal the genetic relationships among these strains in this study. ove ... | 2017 | 28619172 |
| development of a multiplex qpcr in real time for quantification and differential diagnosis of salmonella gallinarum and salmonella pullorum. | currently there are 2659 salmonella serovars. the host-specific biovars salmonella pullorum and salmonella gallinarum cause systemic infections in food-producing and wild birds. fast diagnosis is crucial to control the dissemination in avian environments. the present work describes the development of a multiplex qpcr in real time using a low-cost dna dye (sybr green) to identify and quantify these biovars. primers were chosen based on genomic regions of difference (rod) and optimized to control ... | 2017 | 28589774 |
| o-polysaccharide is important for salmonella pullorum survival in egg albumen, and virulence and colonization in chicken embryos. | the pathogen salmonella pullorum is the causative agent of persistent systemic infection of poultry, leading to economic losses in developing countries due to morbidity, mortality and reduction in egg production. these infections may result in vertical transmission to eggs or progeny. limited information is available regarding the mechanisms involved in the survival of salmonella pullorum in egg albumen and developing chicken embryos. hence, we investigated the role of o-polysaccharide in the co ... | 2017 | 28470101 |
| rapid electrochemical quantification of salmonella pullorum and salmonella gallinarum based on glucose oxidase and antibody-modified silica nanoparticles. | in this article, a facile and sensitive electrochemical method for quantification of salmonella pullorum and salmonella gallinarum (s. pullorum and s. gallinarum) was established by monitoring glucose consumption with a personal glucose meter (pgm). antibody-functionalized magnetic nanoparticles (igg-mnps) were used to capture and enrich s. pullorum and s. gallinarum, and igg-mnps-s. pullorum and igg-mnps-s. gallinarum complexes were magnetically separated from a sample using a permanent magnet. ... | 2017 | 28429065 |
| safety, protective immunity, and diva capability of a rough mutant salmonella pullorum vaccine candidate in broilers. | salmonella enterica subsp. enterica serovar gallinarum biovar pullorum (salmonella pullorum) is highly adapted to chickens causing an acute systemic disease that results in high mortality. vaccination represents one approach for promoting animal health, food safety and reducing environmental persistence in salmonella control. an important consideration is that salmonella vaccination in poultry should not interfere with the salmonellosis monitoring program. this is the basis of the diva (differen ... | 2017 | 28424675 |
| an efficient multiplex pcr-based assay as a novel tool for accurate inter-serovar discrimination of salmonella enteritidis, s. pullorum/gallinarum and s. dublin. | salmonella enterica serovars enteritidis, pullorum/gallinarum, and dublin are infectious pathogens causing serious problems for pig, chicken, and cattle production, respectively. traditional serotyping for salmonella is costly and labor-intensive. here, we established a rapid multiplex pcr method to simultaneously identify three prevalent salmonella serovars enteritidis, pullorum/gallinarum, and dublin individually for the first time. the multiplex pcr-based assay focuses on three genes tcps, ly ... | 2017 | 28360901 |
| identification of the crucial genes in the elimination and survival process of salmonella enterica ser. pullorum in the chicken spleen. | salmonella enterica ser. pullorum is one of the most easily re-infecting pathogens in poultry production because of its mechanism of escaping from immune elimination. we used the transcriptome method to investigate the variation in gene expression in chicken spleen resulting from the interaction between hosts and s. pullorum in the survival process. the expression of various genes related to the maturation and activation of b cells was activated before s. pullorum was eliminated, which might hel ... | 2017 | 28176342 |
| phenotypic and genotypic characterization of locally isolated salmonella strains used in preparation of salmonella antigens in egypt. | this work was conducted to study the phenotypic and genotypic characterization of locally isolated salmonella strains (salmonella pullorum, salmonella enteritidis, and salmonella typhimurium) from poultry used in the preparation of salmonella antigens in egypt. | 2016 | 28096617 |
| identification by pcr signature-tagged mutagenesis of attenuated salmonella pullorum mutants and corresponding genes in a chicken embryo model. | a key feature of the fowl-specific pathogen salmonella pullorum is its vertical transmission to progeny via the egg. in this study, pcr signature-tagged mutagenesis identified nine genes of a strain of s. pullorum that contributed to survival in the chicken embryo during incubation. the genes were involved in invasion, cell division, metabolism and bacterial defence. the competition index in vivo and in vitro together with a virulence evaluation for chicken embryos of all nine mutant strains con ... | 2017 | 28089946 |
| inductive expression of the nod1 signalling pathway in chickens infected with salmonella pullorum. | 1. the aim of this study was to describe the role of nucleotide-binding oligomerization domain-containing protein 1 (nod1) receptor signalling in chicken. 2. tissue-specific expression analysis of nod1, receptor-interacting serine-threonine kinase 2 (ripk2), nuclear factor kappa b (nf-κb) and mitogen-activated protein kinase 11 (mapk11 or p38) by quantitative real-time pcr (qrt-pcr) revealed their wide distribution in various organs and tissues. 3. salmonella pullorum infection activated nod1 re ... | 2017 | 28084794 |
| one-step pcr detection of salmonella pullorum/gallinarum using a novel target: the flagellar biosynthesis gene flhb. | salmonella enterica serovar pullorum/gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. traditional salmonella serotyping is an expensive and time-consuming process. in this study, we developed a rapid one-step polymerase chain reaction (pcr) method to identify s. pullorum/gallinarum. the pcr-based assay focuses on flhb, which shows a deficient region only in s. pullorum/gallinarum, compared with that of other serovars. the specificity and se ... | 2016 | 27920764 |
| salmonella enterica serovar gallinarum: addressing fundamental questions in bacteriology sixty years on from the 9r vaccine. | sixty years on from smith's seminal work on fowl typhoid vaccines, there is renewed interest in experimental avian salmonellosis and in particular the use of salmonella enterica serovar gallinarum as a tool to understand key features of bacterial evolution and host adaptation. in this short review we outline some of the recent advances in avian salmonellosis research that have coupled both the power of whole genome analysis and new tools to understand the host response to existing experimental i ... | 2017 | 27791403 |
| expression patterns of nlrc5 and key genes in the stat1 pathway following infection with salmonella pullorum. | nlrc5, a protein belonging to the nod-like receptor protein family (nlrs), is highly expressed in immune tissues and cells. nlrc5 plays an important role in the immune response of humans, where its regulatory mechanism has been elucidated. however, the function and regulation of nlrc5 in chickens remains unclear. in this study, temporal expression characteristics of nlrc5 and associated genes in the stat1 pathway in chickens following infection with salmonella pullorum were investigated using qu ... | 2017 | 27771450 |
| descriptive survey and salmonella surveillance of pastured poultry layer farms in california. | while pasture-raised poultry comprises a small portion of the commercial poultry industry in north america, these alternative rearing systems have become increasingly popular. as such, it is critical to improve our understanding of husbandry practices and prevalence of zoonotic and epizoonotic diseases in these systems. this research reviews the results of a survey sent to 82 commercial pastured poultry farms in california. while the survey response was low (13.4%), it was enhanced by detailed i ... | 2017 | 27744297 |
| influence of salmonella enterica serovar pullorum pathogenicity island 2 on type iii secretion system effector gene expression in chicken macrophage hd11 cells. | salmonella pathogenicity island 2 (spi2) can encode type iii secretion system 2 (t3ss2) which plays an important role in systemic disease development through delivering different effector proteins into host cells. here, the influence of salmonella pullorum pathogenicity island 2 on t3ss2 effector gene expression was studied using qrt-pcr in chicken macrophage hd11 cells. our results showed that all the detected genes (including pseudogenes sifb, ssph2 and stec) can express in hd11 cells of s. pu ... | 2017 | 27735192 |
| rapid and sensitive detection of mycoplasma synoviae by an insulated isothermal polymerase chain reaction-based assay on a field-deployable device. | mycoplasma synoviae (ms), causing respiratory diseases, arthritis, and eggshell apex abnormalities in avian species, is an important pathogen in the poultry industry. implementation of a biosecurity plan is important in ms infection management. working on a field-deployable pockit™ device, an insulated isothermal polymerase chain reaction (iipcr) assay has a potential for timely ms detection on the farm. the ms iipcr assay had limit of detection 95% of about 9 genome equivalents by testing seria ... | 2017 | 27389062 |
| molecular identification of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum by a duplex pcr assay. | salmonella enterica subsp. enterica serovar gallinarum biovar gallinarum (s gallinarum) and biovar pullorum (s pullorum) are 2 poultry pathogens that cause major economic losses to the poultry industry worldwide. control of both diseases mainly relies on the adoption of biosecurity programs, and success is dependent on accurate and fast detection. based on this concept, we developed a duplex pcr assay, targeting 2 chromosomal sequences, which allowed us to precisely identify and differentiate s ... | 2016 | 27216724 |
| construction and characterization of a cigr deletion mutant of salmonella enterica serovar pullorum. | salmonella enterica serovar pullorum (s. pullorum) is the causative agent of pullorum disease (pd) and results in severe economic losses to the poultry industry. as a salmonella type iii secretion system 2 (t3ss2) effector and predicted membrane protein, cigr is encoded by the cigr gene within salmonella pathogenicity island 3 (spi3). in order to research the influence of the cigr gene on s. pullorum, a cigr mutant of s. pullorum s06004 was constructed by the lambda red recombination system, and ... | 2016 | 27163262 |
| evaluation of protective efficacy of a novel inactivated salmonella pullorum ghost vaccine against virulent challenge in chickens. | salmonella gallinarum biovar pullorum is the causative agent of pullorum disease in poultry, an acute systemic disease that results in a high mortality rate in young chickens. vaccines have been considered in many developing countries where levels of infection are high and eradication is not a realistic option. an attenuated strain combined with protein e-mediated cell lysis was used to generate a safety enhanced salmonella pullorum ghost vaccine. immune responses and protection induced by ghost ... | 2016 | 27090623 |
| serovars and antimicrobial resistance of salmonella spp. isolated from turkey and broiler carcasses in southern brazil between 2004 and 2006. | salmonella spp. causes diseases in fowls, when species-specific serovars (salmonella pullorum and s.gallinarum) are present in flocks, and public health problems, when non-typhoid serovars are isolated, as well as possible bacterial resistance induced by the preventive and therapeutic use of antimicrobials in animal production. this study describes the serovars and bacterial resistance of 280 salmonella spp. strains isolated from turkey and broiler carcasses in southern brazil between 2004 and 2 ... | 2016 | 27007562 |
| myd88 polymorphisms and association with susceptibility to salmonella pullorum. | myeloid differentiation primary response gene 88 (myd88), a universal adapter protein, plays an important role in activating the nuclear factor-κb (nf-κb) and regulating the expression of proinflammatory genes like tumor necrosis factor (tnf) and interleukin-1 (il-1), which were highly involved in salmonella pullorum infection. to detect the relationship between polymorphisms of the myd88 gene and salmonella pullorum disease, we screened the coding region (cds) of the myd88 gene by dna pool cons ... | 2015 | 26881204 |
| a critical role of bacterioferritin in salmonella pullorum-induced ifn-β expression in df-1 cells. | salmonella enterica serovar pullorum (s. pullorum) causes pullorum disease in poultry and results in great economic losses to the poultry industry. although an eradication program has been successfully performed in some countries, it remains a major threat to countries with poor poultry disease surveillance. currently there are no effective control measures for pullorum disease except eradication. in particular, the pathogenesis of s. pullorum infection is still largely unknown. here we identifi ... | 2016 | 26870001 |
| complete genome and methylome sequences of two salmonella enterica spp. | salmonella enterica is responsible for major foodborne outbreaks worldwide. it can cause gastroenteritis characterized by diarrhea, vomiting, and fever. salmonella infections raise public health concerns along with consequential economic impacts. in this report, we announce the first complete genome sequences of salmonella enterica subsp. enterica serovar choleraeuis (s. choleraeuis) atcc 10708 and salmonella enterica subsp. enterica serovar pullorum (s. pullorum) atcc 9120, isolated from patien ... | 2016 | 26798102 |
| [construction and characterization of salmonella pathogenicity island 2 (spi-2) deletion mutant of salmonella pullorum s06004]. | to research the pathogenicity of salmonella pathogenicity island 2 (spi-2) deletion mutant of salmonella pullorum and preliminary explore the feasibility of developing safe attenuated salmonella pullorum candidate vaccine strain. | 2015 | 26762028 |
| retrospective investigation of cataract management in avian species in a zoologic collection. | a review of avian cataracts at the wildlife conservation society's bronx zoo between 1992 and 2011 was conducted. ninety cataracts in 54 birds from 42 species were identified. cataracts were found primarily during examination for ocular abnormalities (29/54, 53.7%) or opportunistically (13/54, 24.1%) and were most commonly diagnosed as mature (22/90, 24.4%). systemic medical conditions diagnosed in these birds included west nile virus (4/54, 7.4%), head trauma (3/54, 5.6%), plumbism and salmonel ... | 2015 | 26667543 |
| a nonenzymatic optical immunoassay strategy for detection of salmonella infection based on blue silica nanoparticles. | a novel nonenzymatic optical immunoassay strategy was for the first time designed and utilized for sensitive detection of antibody to salmonella pullorum and salmonella gallinarum (s. pullorum and s. gallinarum) in serum. the optical immunoassay strategy was based on blue silica nanoparticles (blue-sinps) and magnetic beads (mb). to construct such an optical immunoassay system, the blue-sinps were first synthesized by inverse microemulsion method, characterized by sem, zeta potential and ftir. t ... | 2015 | 26526916 |
| evaluation of risedronate as an antibiofilm agent. | escherichia coli cra null mutants have been reported in the literature to be impaired in biofilm formation. to develop e. coli biofilm-inhibiting agents for prevention and control of adherent behaviour, analogues of a natural cra ligand, fructose-1,6-bisphosphate, were identified based on two-dimensional similarity to the natural ligand. of the analogues identified, those belonging to the bisphosphonate class of drug molecules were selected for study, as these are approved for clinical use in hu ... | 2016 | 26497196 |
| construction of recombinant pichia pastoris carrying a constitutive avbd9 gene and analysis of its activity. | avian beta-defensin 9 (avbd9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, avbd9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. using the preferred codon of pichia pastoris, the mature avbd9 peptide was designed and synthesized, based on th ... | 2015 | 26370794 |
| eric-pcr genotyping of field isolates of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum. | salmonella gallinarum (sg) and salmonella pullorum (sp) have been classified as biovars belonging to salmonella enterica subsp. enterica serovar gallinarum. genetic diversity among isolates of the same biovar can be detected by dna fingerprinting techniques which are useful in epidemiological investigations. in this study, we applied the pcr amplification of enterobacterial repetitive intergenic consensus sequences (eric-pcr) to analyse 45 strains of sg and sp, most of which were isolated from d ... | 2015 | 26365161 |
| immunogenicity and protective efficacy of salmonella enterica serovar pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate. | salmonella enterica serovar pullorum (s. pullorum) causes pullorum disease (pd), a severe systemic disease of poultry and results in considerable economic losses in developing countries. in order to develop a safe and immunogenic vaccine, the immunogenicity and protective efficacy of s06004δspi2, a salmonella pathogenicity island 2 (spi2) deleted mutant of s. pullorum was evaluated in 2-day old chickens. | 2015 | 26205518 |
| [identification of rpoe gene associated with biofilm formation of salmonella pullorum]. | in order to identify sigma factor associated with biofilm formation of salmonella pullorum, we determined the gene expression in the wild type strain and biological characteristics of deletion mutants. | 2015 | 25958695 |
| evaluation of the salmonella enterica serovar pullorum pathogenicity island 2 mutant as a candidate live attenuated oral vaccine. | salmonella enterica serovar pullorum (s. pullorum) is a highly adapted pathogen that causes pullorum disease (pd), an important systemic disease of poultry that causes severe economic losses in developing countries. in the interests of developing a safe and immunogenic oral vaccine, the efficacy of a salmonella pathogenicity island 2 (spi2)-deleted mutant of s. pullorum (s06004δspi2) was evaluated in chickens. s06004δspi2 was severely less virulent than the parental wild-type strain s06004 as de ... | 2015 | 25924763 |
| [prokaryotic expression and application of spic protein of salmonella pullorum]. | to express the spic protein of salmonella pullorum and establish an indirect elisa method with spic protein as antigen. | 2015 | 25652851 |
| identification and characterization of regions of difference between the salmonella gallinarum biovar gallinarum and the salmonella gallinarum biovar pullorum genomes. | salmonella gallinarum is the causative agent of fowl typhoid, a severe septicaemic disease that affects birds of all ages, whereas s. pullorum causes pullorum disease, a systemic disorder affecting primarily young birds. a proportion of birds with pullorum disease become carriers and are thereby able to transmit s. pullorum vertically. although these two pathogens cause distinct diseases, they are otherwise phenotypically and genetically similar. therefore, the small variations that lead to the ... | 2015 | 25497350 |
| identification of isolated salmonella enterica serotype gallinarum biotype pullorum and gallinarum by pcr-rflp. | salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. among salmonella spp., salmonella gallinarum and salmonella. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity. | 2014 | 25485068 |
| effect of dietary supplementation of bacteriophage on performance, egg quality and caecal bacterial populations in laying hens. | 1. bacteriophages (bp) have gained increasing attention as a treatment of bacterial infection for animals. however, the data pertaining to dietary application of bp for laying hens have been limited. 2. this study aimed to investigate the effect of dietary bp on laying performance, egg quality and caecal bacterial populations in laying hens. 3. the dietary bp used in this experiment was a mixture of individual bp targeting salmonella gallinarum, salmonella pullorum, salmonella typhimurium, salmo ... | 2015 | 25471539 |
| cloning, expression, and purification of a new antibacterial substance gene from larvae of musca domestica (diptera: muscidae). | musca domestica l. (diptera: muscidae), the housefly, exhibits unique immune defenses and can produce antibacterial substances upon stimulation with bacteria. on the basis of the cdna library constructed using the suppression subtractive hybridization method, a 1188-bp antibacterial substance gene, which we named as566, was amplified by rapid amplification of cdna ends from m. domestica larva stimulated with salmonella pullorum (enterobacteriaceae: salmonella). in this study, the full-length as5 ... | 2014 | 25434038 |
| immunosensor based on electrodeposition of gold-nanoparticles and ionic liquid composite for detection of salmonella pullorum. | in order to increase the reproducibility and stability of electrochemical immunosensor, which is a key issue for its application and popularization, an accurate and stable immunosensor for rapid detection of salmonella pullorum (s. pullorum) was proposed in this study. the immunosensor was fabricated by modifying screen-printed carbon electrode (spce) with electrodeposited gold nanoparticles (aunps), hrp-labeled anti-s. pullorum and ionic liquids (ils) (aunp/hrp/il). aunps are electrodeposited o ... | 2014 | 25193438 |
| cloning, expression, and purification of a new antimicrobial peptide gene from musca domestica larva. | musca domestica (diptera: muscidae), the housefly, exhibits unique immune defences and can produce antimicrobial peptides upon stimulation with bacteria. based on the cdna library constructed using the suppression subtractive hybridization (ssh) method, a 198-bp antimicrobial peptide gene, which we named mdap-2, was amplified by rapid amplification of cdna ends (race) from m. domestica larvae stimulated with salmonella pullorum (enterobacteriaceae: salmonella). in the present study, the full-len ... | 2014 | 25020259 |
| draft genome sequence of salmonella enterica subsp. enterica serovar gallinarum biovar pullorum strain fcav198, a brazilian chicken pathogen. | salmonella enterica subsp. enterica serovar gallinarum biovar pullorum is a bird-restricted pathogen which causes pullorum disease. the strain fcav198 was isolated from a pool of chicken ovaries in brazil, and its genome may be helpful for studies involving molecular mechanisms related to pathogenesis and other related applications. | 2014 | 24558231 |
| virulence determinants of salmonella gallinarum biovar pullorum identified by pcr signature-tagged mutagenesis and the spic mutant as a candidate live attenuated vaccine. | salmonella gallinarum biovar pullorum (s. gallinarum biovar pullorum) is the causative agent of pullorum disease (pd) in chickens which results in considerable economic losses to the poultry industries in developing countries. pcr-signature tagged mutagenesis was used to identify virulence determinants of s. gallinarum biovar pullorum and novel attenuated live vaccine candidates for use against this disease. a library of 1800 signature-tagged s. gallinarum biovar pullorum mutants was constructed ... | 2014 | 24355532 |
| development of a novel hexa-plex pcr method for identification and serotyping of salmonella species. | salmonella is one of the most important foodborne pathogens, which causes a huge economic burden worldwide. to detect salmonella rapidly is very meaningful in preventing salmonellosis and decreasing economic losses. currently, isolation of salmonella is confirmed by biochemical and serobased serotyping methods, which are time consuming, labor intensive, and complicated. to solve this problem, a hexa-plex polymerase chain reaction (pcr) method was developed using comparative genomics analysis and ... | 2014 | 24066904 |
| molecular differentiation between salmonella enterica subsp enterica serovar pullorum and salmonella enterica subsp enterica serovar gallinarum. | s. pullorum (sp) and s. gallinarum (sg) are very similar. they are the agents of pullorum disease and fowl typhoid, respectively, and the two diseases are responsible for economic losses in poultry production. although sp and sg are difficult to be differentiated in routine laboratory procedures, the ability to metabolize ornithine is a biochemical test that may be used to achieve this aim. while sp is able to decarboxylate this amino acid, sg is not. however, the isolation of strains showing at ... | 2009 | 24031341 |
| serosurvey for selected infectious agents in two sympatric species of cormorants (phalacrocorax atriceps and phalacrocorax magellanicus) from coastal patagonia, argentina. | we conducted a serologic survey for selected infectious agents on two sympatric cormorants, the imperial cormorant (phalacrocorax atriceps) and the rock shag (phalacrocorax magellanicus). blood was collected from 267 imperial cormorants and 106 rock shags at 17 colonies along the patagonia atlantic shore during nine breeding seasons (1994, 1999-2001-2005-2008-2010). antibodies to four pathogens were common to both species and frequently observed: avian paramyxovirus type 1 (56% of imperial cormo ... | 2013 | 23778597 |
| identification of salmonella enterica serovar pullorum antigenic determinants expressed in vivo. | salmonella enterica serovar pullorum affecting poultry causes pullorum disease and results in severe economic loss in the poultry industry. currently, it remains a major threat in countries with poor poultry surveillance and no efficient control measures. as s. pullorum could induce strong humoral immune responses, we applied an immunoscreening technique, the in vivo-induced antigen technology (iviat), to identify immunogenic bacterial proteins expressed or upregulated during s. pullorum infecti ... | 2013 | 23774596 |
| comparison of 3 culture methods and pcr assays for salmonella gallinarum and salmonella pullorum detection in poultry feed. | to detect salmonella gallinarum or salmonella pullorum in artificially contaminated poultry feed, 9 culture combinations were compared, including 3 preenrichment/enrichment methods (tryptic soy broth plus ferrous sulfate/tetrathionate hajna, tryptic soy broth plus ferrous sulfate/selenite cystine broth, and salmosyst) in combination with 3 selective agars (xylose lysine desoxicholate agar added with tergitol 4, ef-18, and önöz), respectively. additionally, a single pcr technique was applied comb ... | 2013 | 23687146 |
| antimicrobial resistance, presence of integrons and biofilm formation of salmonella pullorum isolates from eastern china (1962-2010). | three hundred and thirty-seven isolates of salmonella pullorum from eastern china between 1962 and 2010 were characterized for antimicrobial susceptibility (disk diffusion method), the presence of integrons (polymerase chain reaction followed by sequencing) and the ability to form biofilms (semi-quantitative adherence assay). two hundred and fifty-eight isolates (76.6%) exhibited multiple drug resistance (mdr; resistant to at least three different classes of antimicrobials), and the level of dru ... | 2013 | 23656571 |
| polymerase chain reaction assay based on rata gene allows differentiation between salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum. | salmonella pullorum and salmonella gallinarum are classified as biovars of salmonella enterica subsp. enterica serovar gallinarum. these salmonellae are the causative agents of pullorum disease and fowl typhoid, respectively, and are widely distributed throughout the world. although many developed countries have eradicated these diseases from commercial poultry, they are still the cause of significant economic loss in developing countries. when serovar gallinarum is isolated, it is difficult to ... | 2013 | 23512922 |
| serologic, parasitic, and bacteriologic assessment of captive cracids (aves: galliformes: cracidae) in brazil. | captive cracids (aves: galliformes: cracidae), including endangered species, were studied (n = 130) for the assessment of health status, including aburria jacutinga (black-fronted piping-guan, n = 42), crax blumenbachii (red-knobbed curassow, n = 54), craxfasciolata (bare-faced curassow, n = 28), and penelope obscura (dusky-legged guan, n = 6). the exposure to mycoplasma gallisepticum (mg), mycoplasma synoviae (ms), salmonella pullorum (sp), salmonella gallinarum (sg), avian paramyxovirus-1 (apm ... | 2013 | 23505700 |
| [construction of a temperature-sensitive and replication-defective t-vector and its application for gene knockout in salmonella pullorum]. | knockout is an important method for gene function study, while vector is the core of gene knockout. in order to obtain an effective vector for rapid construction of mutant and essentiality identification of the corresponding gene, we constructed a recombinant plasmid named pldm-t based on the temperature-sensitive and replication- defective plasmid pldm1 by inserting an xcm i adapter into the ecor i and pst i sites of pidm1. digesting with xcm i, pidm-t can be prepared as a linear t-vector for p ... | 2012 | 23289313 |
| a comparative study of culture methods and pcr assay for salmonella detection in poultry drinking water. | the present work compared 2 culture methods and pcr assays for motile and nonmotile salmonella detection using artificially contaminated poultry drinking water. the specificity was 1 for all methods studied. the accuracy and sensitivity were 1 for all motile strains, whereas these parameters were between 0 and 0.7 for nonmotile salmonella strains. the positive predictive value and negative predictive value were 1 for all motile salmonella strains in the 3 methods used. nonmotile salmonella strai ... | 2013 | 23243252 |
| health assessment of captive tinamids (aves, tinamiformes) in brazil. | ninety-five (95) captive tinamids (aves, tinamiformes) of species crypturellus obsoletus (brown tinamou), crypturellus parvirostris (small-billed tinamou), crypturellus tataupa (tataupa tinamou), crypturellus undulatus (undulated tinamou), rhynchotus rufescens (red-winged tinamou), and tinamus solitarius (solitary tinamou) were evaluated for diseases of mandatory control in the brazilian poultry health program (pnsa). antibodies were detected by serum agglutination test (sat) in 4 birds for myco ... | 2012 | 23082518 |
| the long view: salmonella--the last forty years. | as a part of the 40th anniversary celebrations of avian pathology we review the last four decades of salmonella research which has led to major progress in our understanding of the bacteriology and infection biology of the organism through the huge advances in molecular biology and immunology that have accompanied technical advances in biology generally. in many countries combinations of improvements in management, sometimes under legislative pressure and supported by a number of basic biologica ... | 2012 | 23025669 |
| protective effects of zinc-bearing clinoptilolite on broilers challenged with salmonella pullorum. | this study was conducted to evaluate the effects of zinc-bearing clinoptilolite (zncp) on broilers challenged with salmonella pullorum for a 21-d feeding trial. a total of 240 one-day-old chickens was allocated into 4 treatment groups: 1) nonchallenged control (con) fed basal diet; 2) s. pullorum-challenged control (scc) fed basal diet; 3) salmonella challenge fed basal diet plus antibiotic (ant); 4) salmonella challenge fed basal diet plus 0.2% zncp (zncp). compared with con, salmonella challen ... | 2012 | 22802176 |
| differential identification of salmonella enterica serovar gallinarum biovars gallinarum and pullorum and the biovar gallinarum live vaccine strain 9r. | salmonella enterica serovar gallinarum biovar gallinarum causes fowl typhoid in chickens and has been of economic importance to the chicken industry in many countries. the biovar gallinarum live vaccine strain 9r (sg 9r) has been used to control fowl typhoid in many areas where the disease is endemic. therefore, a definitive diagnosis of this disease may require differentiation of wild-type field isolates of biovar gallinarum from the live vaccine strain sg 9r. here, we report the development of ... | 2012 | 22728122 |
| early immune dynamics following infection with salmonella enterica serovars enteritidis, infantis, pullorum and gallinarum: cytokine and chemokine gene expression profile and cellular changes of chicken cecal tonsils. | salmonella enterica subspecies enterica infection remains a serious problem in a wide range of animals and in man. poultry-derived food is the main source of human infection with the non-host-adapted serovars while fowl typhoid and pullorum disease are important diseases of poultry. we have assessed cecal colonization and immune responses of newly hatched and older chickens to salmonella serotypes enteritidis, infantis, gallinarum and pullorum. s. enteritidis and s. infantis colonized the ceca m ... | 2012 | 22512820 |
| immune dynamics following infection of avian macrophages and epithelial cells with typhoidal and non-typhoidal salmonella enterica serovars; bacterial invasion and persistence, nitric oxide and oxygen production, differential host gene expression, nf-κb signalling and cell cytotoxicity. | poultry-derived food is a common source of infection of human with the non-host-adapted salmonellae while fowl typhoid and pullorum disease are serious diseases in poultry. development of novel immune-based control strategies against salmonella infection necessitates a better understanding of the host-pathogen interactions at the cellular level. intestinal epithelial cells are the first line of defence against enteric infections and the role of macrophages is crucial in salmonella infection and ... | 2012 | 22475571 |
| influence of truncation of avian β-defensin-4 on biological activity and peptide-membrane interaction. | defensins are important components in host defense systems. the therapeutic use of β-defensins is limited by their innate toxicity and high cost due to the size and complex disulfide pairing. in this study, we used linear avian β- defensin-4 (rl38) without disulfide bonds as model peptide to derive two peptides by the truncation. gl23 is the c-terminal truncated sequence of rl38, and gli23 is the derivative of gl23 by the replacement of cysteines with isoleucines. results showed that these pepti ... | 2012 | 22316306 |
| complete genome sequence of salmonella enterica serovar pullorum rks5078. | salmonella enterica serovar pullorum is a chicken-adapted pathogen, causing pullorum disease. its strict host adaptation has been suspected to result in gene decay. to validate this hypothesis and identify the decayed genes, we sequenced the complete genome of s. pullorum rks5078. we found 263 pseudogenes in this strain and conducted functional analyses of the decayed genes. | 2012 | 22247537 |
| effects of hydrolysed saccharomyces cerevisiae yeast and yeast cell wall components on live performance, intestinal histo-morphology and humoral immune response of broilers. | 1. the effects of enzymatically hydrolysed whole saccharomyces cerevisiae yeast (hy) and the pellets of yeast cell wall (ycw) on production traits, the microbiology and histo-morphology of the small intestine, and humoral immune responses against newcastle disease virus (ndv), of ross 308 broilers were investigated. 2. the control group received a maize-soyabean meal based basal diet for 42 days. in the treated groups the basal diet was supplemented with 1 g/kg of hy and ycw. there were 8 repl ... | 2011 | 22221235 |
| Differential gene expression of antimicrobial peptides ß defensins in the gastrointestinal tract of Salmonella serovar Pullorum infected broiler chickens. | Salmonella enterica serovar Pullorum causes substantial mortality in chicks as well as results in persistent infection and vertical transmission in layer birds. An effective innate immune response in the early stages of infection could reduce bacterial colonization and mortality in chicks and persistency of infection in later stages. ß Defensins (AvBDs) are now considered as one of the key components of innate immunity in avian species. In the present study, we quantified the mRNA expression lev ... | 2011 | 22170093 |
| isolation and characterization of bacteriophages of salmonella enterica serovar pullorum. | in this study, 2 bacteriophages of salmonella pullorum were isolated using an enrichment protocol and the double agar layer method. they were named pspu-95 and pspu-4-116, respectively, against clinical isolates of salmonella pullorum spu-95 and spu-116. the host ranges of the 2 bacteriophages were determined by performing spot tests with 20 bacteria strains. both bacteriophages had wide host ranges. bacteriophage pspu-95 had a lytic effect on 17 of the 20 isolates (85%), and pspu-4-116 produced ... | 2011 | 21934022 |
| lactobacillus reuteri atcc 55730 and l22 display probiotic potential in vitro and protect against salmonella-induced pullorum disease in a chick model of infection. | lactobacillus reuteri atcc 55730 (l. reuteri atcc 55730) and l. reuteri l22 were studied for their probiotic potential. these two strains were able to produce an antimicrobial substance, termed reuterin, the maximum production of reuterin by these two strains was detected in the late logarithmic growth phase (16h in mrs and 20h in lb broths). these two strains could significantly reduce the growth of salmonella pullorum atcc 9120 in mrs broth, l. reuteri atcc 55730 with a reduction of 48.2±4.15% ... | 2011 | 21764090 |
| population dynamics of salmonella enterica serotypes in commercial egg and poultry production. | fresh and processed poultry have been frequently implicated in cases of human salmonellosis. furthermore, increased consumption of meat and poultry has increased the potential for exposure to salmonella. while advances have been made in reducing the prevalence and frequency of salmonella contamination in processed poultry, there is mounting pressure on commercial growers to prevent and/or eliminate these human pathogens in preharvest production facilities. several factors contribute to salmonell ... | 2011 | 21571882 |
| assessment of yeast cell wall as replacements for antibiotic growth promoters in broiler diets: effects on performance, intestinal histo-morphology and humoral immune responses. | the study compared the effects of an antibiotic growth promoter (agp), yeast (saccharomyces cerevisiae) and yeast cell wall (ycw) on performance, microbiology and histo-morphology of the small intestine and humoral immune responses in ross 308 broilers. the treatments (eight replicates/treatment, n = 12/replicate) were negative control (nc, without agp), positive control (pc, supplemented with bacitracin methylene disalicylate, 400 mg/kg), y and ycw (supplemented with yeast and ycw, respectively ... | 2011 | 21535233 |
| pullorum disease and fowl typhoid--new thoughts on old diseases: a review. | fowl typhoid and pullorum disease are two distinct septicaemic diseases largely specific to avian species and caused by salmonella gallinarum and salmonella pullorum, respectively. they were first described more than one century ago. since their discovery, many efforts have been made to control and prevent their occurrence in commercial farming of birds. however, they remain a serious economic problem to livestock in countries where measures of control are not efficient or in those where the cli ... | 2011 | 21331943 |
| [cloning and identification of ipaj gene in salmonella pullorum]. | cloning of ipaj gene from salmonella pullorum c79-13, and identification of expressed ipaj protein as an immunogen of the pathogen. | 2010 | 21268902 |
| differential identification of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum based on polymorphic regions of glgc and spec genes. | salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum cause fowl typhoid and pullorum disease in avian species, respectively, and have been of considerable economic importance to the poultry industry in parts of the world. the definitive diagnosis of these diseases can be made only by isolation and identification of the causative agent. however, rapid identification of biovars gallinarum and pullorum is not easily feasible due to their common antigenic structure ... | 2010 | 21111918 |
| pullorum disease of turkeys. | 1945 | 21028715 | |
| note on salmonella in poultry products. | 1946 | 21024769 | |
| studies in pullorum disease; the possible relationship of non-specific reactions to the sensitizing influence of ingested proteins. | 1946 | 21020413 | |
| sulfamerazine as a prophylactic in pullorum disease in poults. | 1946 | 21014215 | |
| pullorum disease; historical sketch. | 1946 | 21009454 | |
| studies in pullorum disease; transmission of infection to healthy birds by contact. | 1945 | 21008177 | |
| studies in pullorum disease; the effect of sulfamerazine on artificially and naturally infected chicks. | 1946 | 21000922 | |
| studies in pullorum disease; bacteriological examination of tissues from fowls exposed to infection with salmonella pullorum. | 1946 | 21000921 | |
| studies on pullorum disease using various rapid antigens. | 1946 | 20998646 | |
| an outbreak of gastroenteritis presumably caused by salmonella pullorum. | 1946 | 20996928 | |
| a survey of avian salmonellosis in great britain. | 1946 | 20990781 | |
| sulfamerazine in the treatment of pullorum disease. | 1946 | 20988123 | |
| difference and variation of the sef14 operon gene clusters in salmonella pullorum. | sef14 fimbriae are only found in some strains of serogroup-d salmonella such as s. enteritidis, suggesting that sef14 fimbriae may affect serovar-specific virulence traits. in this study, we found that prevalence of sefa, sefd and sefr genes in s. dublin and s. enteritidis was 100%. in 18 isolates of s. pullorum, the prevalence of sefa gene was 100%, while the prevalence of sefd and sefr genes was 38.9% (7/18), and 11 strains isolated after 1980s did not contain any gene sefd or sefr. interestin ... | 2010 | 20806242 |
| monocytes-macrophages phagocytosis as a potential marker for disease resistance in generation 1 of dwarf chickens. | monocytes-macrophages play an indispensable role in the immune system. the current study investigated the effect of selection for monocytes-macrophages phagocytosis on disease resistance in generation 1 (g1) of dwarf chickens. five hundred dwarf chickens of generation 0 (g0) were divided into high and low phagocytic index (pi) groups (hpig and lpig, respectively) based on their pi of monocytes-macrophages at 290 d of age. then, 2 x 2 mating combinations were conducted. sixty g0 chickens from ano ... | 2010 | 20709989 |
| testing for salmonella spp. in released parrots, wild parrots, and domestic fowl in lowland peru. | wild animal populations face threats from pathogens from both intentionally released captive animals and domestic animals that accompany human settlements. from december 2004 through august 2005, we studied free living macaws and parrots in the tambopata national reserve in the peruvian amazon and semicaptive domestic fowl in human settlements adjacent to the reserve. in 1992-1993, large macaws (aras spp.) that were serologically positive for salmonella pullorum were released into this reserve, ... | 2010 | 20688677 |
| isolation of salmonella enterica serovar enteritidis in blue-fronted amazon parrot (amazona aestiva). | avian salmonellosis is a disease caused by bacteria of the genus salmonella that can cause three distinct diseases in birds: pullorum diseases, fowl typhoid, and paratyphoid infection. various wildlife species are susceptible to infections by salmonella, regardless of whether they live in captivity or freely in the wild. the present study verified the presence of salmonella enterica serovar enteritidis in three captive specimens of amazona aestiva. the study involved a total of 103 birds undergo ... | 2010 | 20408416 |
| report of a relatively severe and protracted diarrhea presumedly due to salmonella pullorum from the ingestion of incompletely cooked eggs. | 1947 | 20340619 | |
| field studies of sulfamerazine in the control of pullorum and some other diseases of domestic birds. | 1947 | 20295942 | |
| non-specific pullorum agglutination reactions; post mortem studies on fowl exhibiting non-specific reactions over an extended period. | 1947 | 20295900 | |
| studies in pullorum disease; disinfection of incubators with propylene and triethylene glycol. | 1947 | 20295898 | |
| cultivation of the virus of infectious bronchitis of chickens in embryonated chicken eggs. | 1947 | 20295891 | |
| studies in pullorum disease; antigenic differences in strains of salmonella pullorum. | 1947 | 20295890 | |
| the isolation of s. typhimurium from fowls which gave a positive agglutination test with s. pullorum antigen. | 1947 | 20292282 | |
| selection of suitable antigenic strains of s. pullorum by single colony isolation. | 1947 | 20290825 | |
| form variation in salmonella pullorum and its relation to x strains. | 1946 | 20289307 | |
| studies in pullorum disease; trial modifications of tube and stained whole blood antigens. | 1947 | 20283507 | |
| non-specific pullorum agglutination reactions; preliminary observations on fowl exhibiting non-specific reactions over an extended period. | 1946 | 20283504 | |
| studies in pullorum disease; infection in the male and experiments on transmission to the female. | 1946 | 20283503 | |
| streptomycin in the treatment of pullorum in baby chicks. | 1947 | 20279633 | |
| pullorum disease; diagnosis and immunization. | 1946 | 20275970 | |
| studies in pullorum disease; proteus antisera with special reference to the differentiation of regular and variant forms of salmonella pullorum. | 1947 | 20273462 |