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a case of tetraplegia after proteus mirabilis infection. 201728855782
lactococcus lactis as a safe and inexpensive source of bioactive silver composites.this research develops a safe, inexpensive, and more accessible source for synthesis of silver nanoparticles. the bioactive silver composites synthesized by lactococcus lactis 56 ky484989 (lclb56-agcs) were characterized by various physico-chemical techniques and investigated for their antimicrobial activity and cytotoxicity. the average amount of nanoparticles was 0.363 ± 0.09 mg from 50 ml of culture medium. the synthesis efficiency varied from 71 to 85%. synthesized silver nanoparticles with ...201728842740
postoperative nasal septal abscess following use of 2-octylcyanoacrylate and polydioxanone plate in open septorhinoplasty: a case series.the employment of 2-octylcyanoacrylate adhesive (dermabond) to assist graft placement in open septorhinoplasty has been increasingly used to stabilize cartilage grafts. literature regarding this application has been mixed, with some showing the possibility of increased rates of inflammation, if not infection. we present an original comparative case series involving postoperative septal abscess formation adjacent to caudal strut grafting where both dermabond and an absorbable polydioxanone (pds) ...201728831833
evaluation of biofilm-induced urinary infection stone formation in a novel laboratory model system.infection stones comprise approximately 15% of all urinary tract stones and are induced by infection with urease-positive pathogens. the bacteria within the stone matrix present significant treatment impediments compared to metabolic kidney stones. while much is known about how urinary composition regulates metabolic stone formation, there is a general lack of knowledge around which urinary factors regulate the rate of infection stone formation. unfortunately, more in-depth research into infecti ...201728827106
gc-ms analysis and gastroprotective evaluations of crude extracts, isolated saponins, and essential oil from polygonum hydropiper l.peptic ulceration is among the most prevalent gastrointestinal disorders characterized by pepsin and gastric acid mediated mucosal damage, as result of imbalance between defensive and offensive processes. the main objective of the current study was to investigate the antiulcer potentials of polygonum hydropiper crude methanolic ectract (ph.cr) in aspirin induced ulcerogenesis using pylorus ligated rat model. in-vitro urease and proteus mirabilis inhibitory potentials were evaluated using standar ...201728824906
chemical composition of mentha pulegium and rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities.the aim of the study was the determination of the chemical composition of mentha pulegium l. and rosmarinus officinalis l. essential oils and the evaluation of their antileishmanial, antibacterial and antioxidant activities. essential oils (eos) were isolated using steam distillation and the chemical composition was determined using gc-ms analysis. the antibacterial activity was tested against ten pathogenic strains using the diffusion method, the minimum inhibitory concentration (mic) and the m ...201728821401
[to the state of the fetoplacental complex in women with the threat of abortion].the goal is a comprehensive study of the morphofunctional state of the mother-placenta-fetus system in placental insufficiency and infection. 250 pregnant and puerperas for the period 2008-2014 were examined. detection of bacterial flora was carried out in 35 pregnant women aged 20.4±0.8 years with the threat of abortion from an early gestation period and a prolonged persistent infection of the genitals, bacterial vaginosis. methods are applied: culture, ion chromatography, gas-liquid chromatogr ...201728820412
prevalence study of plasmid-mediated ampc β-lactamases in enterobacteriaceae lacking inducible ampc from saudi hospitals.enterobacteriaceae encoding plasmid-mediated ampc (pampc) β-lactamases confer resistance to the third generation cephalosporins. pampc association with extended spectrum β-lactamases (esbls), plasmid-mediated quinolone resistance (pmqr) and aminoglycoside modifying enzymes (ames) is well documented. there are limited data regarding the epidemiology and clinical significance of pampc in saudi arabia. this study aimed to determine the prevalence of pampc and its coexistence with esbls, pmqr and am ...201728820112
fungal bezoar: a rare cause of ureteral obstruction.a 52-year-old male, with diabetes mellitus and alcoholic liver disease, presented to the emergency room for right flank pain of 3 days' duration, associated with dysuria. physical examination revealed right flank tenderness with fever and hypotension; laboratory findings showed acute kidney injury and large blood and leucocytes in the urine. a ct abdomen and pelvis showed hydronephrosis of the right collecting system of a horseshoe kidney with air and hyperdense debris in the renal pelvis. patie ...201728804662
evolving beta-lactamase epidemiology in enterobacteriaceae from italian nationwide surveillance, october 2013: kpc-carbapenemase spreading among outpatients.extended-spectrum beta-lactamases (esbls), ampc-type beta-lactamases (acbls) and carbapenemases are among the most important resistance mechanisms in enterobacteriaceae. this study investigated the presence of these resistance mechanisms in consecutive non-replicate isolates of escherichia coli (n = 2,352), klebsiella pneumoniae (n = 697), and proteus mirabilis (n = 275) from an italian nationwide cross-sectional survey carried out in october 2013. overall, 15.3% of isolates were non-susceptible ...201728797330
camp receptor protein regulates mouse colonization, motility, fimbria-mediated adhesion, and stress tolerance in uropathogenic proteus mirabilis.cyclic amp receptor protein (crp) is a major transcriptional regulator in bacteria. this study demonstrated that crp affects numerous virulence-related phenotypes, including colonization of mice, motility, fimbria-mediated adhesion, and glucose stress tolerance in uropathogenic proteus mirabilis. diabetic mice were more susceptible to kidney colonization by wild-type strain than nondiabetic mice, in which the crp mutant exhibited increased kidney colonization. loss of crp or addition of 10% gluc ...201728779108
antimicrobial activity of endophytic bacterial populations isolated from medical plants of iran.endophytic actinobacteria colonize inside the plant tissues without causing damages to the host plant. since these microorganisms colonize in the different parts of plants and can stop plant disease, they have been applied as biological agents for controlling human diseases. the aim of this study was molecular identification and measuring the antimicrobial activity of endophytic actinomycetes isolated from medicinal plants of iran.201728775818
bacterial species and mycotoxin contamination associated with locust bean, melon and their fermented products in south-western nigeria.the microbiological safety of spontaneously fermented foods is not always guaranteed due to the undefined fermenting microbial consortium and processing materials. in this study, two commonly consumed traditional condiments (iru and ogiri) and their respective raw seeds (locust bean and melon) purchased from markets in south-western nigeria were assessed for bacterial diversity and mycotoxin contamination using 16s rrna gene sequencing and liquid chromatography tandem mass spectrometry (lc-ms/ms ...201728759798
a novel type 1/2 hybrid incc plasmid carrying fifteen antimicrobial resistance genes recovered from proteus mirabilis in china.incc plasmids are of great concern as vehicles of broad-spectrum cephalosporins and carbapenems resistance genes blacmy and blandm. the aim of this study was to sequence and characterize a multidrug resistance (mdr) incc plasmid (ppm14c18) recovered from proteus mirabilis. ppm14c18 was identified in a cmy-2-producing p. mirabilis isolate from chicken in china in 2014, and could be transferred to escherichia coli conferring an mdr phenotype. whole genome sequencing confirmed ppm14c18 was a novel ...201728757095
antibiofilm potential of synthetic 2-amino-5-chlorobenzophenone schiff bases and its confirmation through fluorescence microscopy.antibacterial/antibiofilm potential of microwave-assisted synthetic thirty-three 2-amino-5-chloro benzophenone schiff bases have been carried out against four bacterial strains i.e. klebsiella pneumoniae, proteus mirabilis, staphylococcus aureus and streptococcus mutans. among them compounds 5, 6, 8, 9, 14, 16, 22, 24, 26, and 30-32 showed antibiofilm activities against isolates at less than 100 μg/ml concentrations. these compounds showed enhanced antibiofilm activity against s. aureus as compa ...201728755874
colorimetric sensing of toxic metal and antibacterial studies by using bioextract synthesized silver nanoparticles.here, we report the simple and cost effective colorimetric technique for the determination of toxic metals (hg(2+)) in aqueous sample by using bioextract silver nanoparticles (agnps). the indigenous agnps were synthesised by green and ecologically friendly style using extract of fig (ficus carica) leaf. the synthesized agnps were confirmed by uv-vis spectroscopy, ft-ir spectroscopy, and scanning electron microscopy methods. the synthesis of agnps was observed by its colour changing from light ye ...201728748516
assessment of facultative anaerobes from the root canals of deciduous molars: an in vivo study.background. the current research aimed to assess the prevalence of facultative anaerobes isolated from the root canals of deciduous molars. methods. the present research enrolled 60 children in the 6‒9-year age group based on clinical and radiographic findings. under aseptic conditions, access cavities were prepared followed by collection of samples from infected root canals with the help of sterile paper points. the samples thus obtained were subjected for microbial assay. results. it was found ...201728748050
antimicrobial property of lemongrass (cymbopogon citratus) oil against pathogenic bacteria isolated from pet turtles.the usage of essential oils as antimicrobial agents is gaining attention. besides, pet turtles were known to harbor a range of pathogenic bacteria while the turtle keeping is a growing trend worldwide.the current study examined the antimicrobial activity of lemon grass oil (lgo) against seven species of gram negative bacteria namely; aeromonas hydrophila, a. caviae, citrobacter freundii, salmonella enterica, edwardsiella tarda, pseudomonas aeruginosa, and proteus mirabilis isolated from three po ...201728747972
chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans.the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ...201728725151
a shape-shifting redox foldase contributes to proteus mirabilis copper resistance.copper resistance is a key virulence trait of the uropathogen proteus mirabilis. here we show that p. mirabilis scsc (pmscsc) contributes to this defence mechanism by enabling swarming in the presence of copper. we also demonstrate that pmscsc is a thioredoxin-like disulfide isomerase but, unlike other characterized proteins in this family, it is trimeric. pmscsc trimerization and its active site cysteine are required for wild-type swarming activity in the presence of copper. moreover, pmscsc ex ...201728722010
imipenem-resistant gram-negative bacterial isolates carried by persons upon medical examination in korea.carbapenem-resistant gram-negative bacteria (cr-gnb) have emerged and disseminated worldwide, become a great concern worldwide including korea. the prevalence of fecal carriage of imipenem-resistant gram-negative bacteria (ir-gnb) in persons in korea was investigated. stool samples were collected from 300 persons upon medical examination. samples were screened for ir-gnb by using macconkey agar with 2 μl/ml imipenem. species were identified by 16s rrna gene sequence analysis, and antimicrobial s ...201728721477
accelerated detection of extended-spectrum β-lactamases in clinical isolates of enterobacteriaceae.a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum β-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esblproducers by the standard clinical laboratory standards ...200628700883
propensity-matched analysis of the impact of extended-spectrum β-lactamase production on adults with community-onset escherichia coli, klebsiella species, and proteus mirabilis bacteremia.the presence of extended-spectrum β-lactamase (esbl) in escherichia coli, klebsiella species, and proteus mirabilis (ekp) is of great microbiological and clinical importance. the study dealing with the direct impact of esbl producers on the outcome of patients with community-onset bacteremia is lacking.201728698042
clinical benefits of antimicrobial de-escalation in adults with community-onset monomicrobial escherichia coli, klebsiella species and proteus mirabilis bacteremia.the clinical benefits of an antimicrobial de-escalation strategy were compared with those of a no-switch strategy in bacteremic patients. adults with community-onset monomicrobial escherichia coli, klebsiella species and proteus mirabilis bacteremia treated empirically using broad-spectrum beta-lactams, including third-generation cephalosporins (gcs), fourth-gc or carbapenems, were treated definitively with first- or second-gcs (de-escalation group), the same regimens as empirical antibiotics (n ...201728694235
antibiofilm potential of 16-oxo-cleroda-3, 13(14) e-diene-15 oic acid and its five new γ-amino γ-lactone derivatives against methicillin resistant staphylococcus aureus and streptococcus mutans.the clerodane diterpenoids 16-oxo-cleroda-3, 13(14) e-diene-15 oic acid (1) and kolavenic acid (2) isolated from polyalthia longifolia var. pendula (linn.) were previously reported for their antimicrobial activity. thus present study was designed to investigate the biofilm inhibiting potential of these diterpenoids (1-2) and five new lactone derivatives (3-7) of 1 against methicillin resistant staphylococcus aureus (mrsa), streptococcus mutans, klebsiella pneumoniae and proteus mirabilis. compou ...201728692914
septic arthritis of the pubis symphysis: clinical and therapeutic features.septic arthritis of the pubis symphysis is rare and difficult to diagnose. the objective of our study was to describe the biological, clinical, radiological and therapeutic aspects of this disease. this is a retrospective study of 4 cases of septic arthritis of the pubic symphysis collected in the department of rheumatology and orthopaedics in sousse in tunisia over a period of 16 years (2000-2016). our population consists of 3 women and one men. the mean age was 47 years (18-83). clinical signs ...201728690730
expression, purification, and characterization of a membrane-bound d-amino acid dehydrogenase from proteus mirabilis jn458.to characterize a novel membrane-bound d -amino acid dehydrogenase from proteus mirabilis jn458 (pmdad).201728676939
[proteus mirabilis septic arthritis].acute septic arthritis is rare. it is associated with poor prognosis in terms of mortality and morbidity. we report the case of a 61-year old patient with spontaneous proteus mirabilis septic arthritis. he suffered from complicated diabetes associated with positive blood cultures and synovial fluid cultures. patient's evolution was favorable thanks to early diagnosis and initiation of adequate antibiotic therapy. proteus mirabilis septic arthritis is rare. on that basis we conducted a literature ...201728674590
the distribution of carbapenem- and colistin-resistance in gram-negative bacteria from the tamil nadu region in india.the occurrence of carbapenem- and colistin-resistance among gram-negative bacteria is increasing worldwide. the aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in tamil nadu, india.201728671537
prophylactic use of intraoperative vancomycin powder and postoperative infection: an analysis of microbiological patterns in 1200 consecutive surgical cases.objective wound infections following spinal surgery for deformity place a high toll on patients, providers, and the health care system. the prophylactic application of intraoperative vancomycin powder has been shown to lower the infection risk after thoracolumbar decompression and fusion for deformity correction. the purpose of this study was to assess the microbiological patterns of postoperative surgical site infections (ssis) after prophylactic use of vancomycin powder in adult patients under ...201728665245
monitoring in vitro antibacterial efficacy of 26 indian spices against multidrug resistant urinary tract infecting bacteria.to screen methanolic extracts of 26 commonly used indian spices against nine species of uropathogenic bacteria (enterococcus faecalis, staphylococcus aureus, acinetobacter baumannii, citrobacter freundii, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa), isolated from clinical samples of a tertiary care hospital for antibacterial activity.201428664089
bacterial genus is a risk factor for major amputation in patients with diabetic foot.to evaluate whether bacterial genus is a risk factor for major amputation in patients with diabetic foot and infected ulcer.201728658333
the antimicrobial spectrum of xeroform(®).xeroform(®) is a petrolatum-based fine mesh gauze containing 3% bismuth tribromophenate. bismuth, similar to other metals, has antimicrobial properties. xeroform(®) has been used for decades in burn and plastic surgery as a donor site dressing and as a covering for wounds or partial thickness burns. despite this, the antimicrobial spectrum of xeroform(®) remains largely unknown. we examined the in-vitro efficacy of xeroform(®) against common burn pathogens using zone-of-inhibition methodology in ...201728641915
oral immunisation with taishan pinus massoniana pollen polysaccharide adjuvant with recombinant lactococcus lactis-expressing proteus mirabilis ompa confers optimal protection in mice.proteus mirabilis poses a critical burden on the breeding industry, but no efficient vaccine is available for animals.201728629671
spectrum and antibiotic resistance of uropathogens between 2004 and 2015 in a tertiary care hospital in hungary.surveillance of the bacterial spectrum and antibiotic-resistance patterns of locally occurring uropathogens is essential to serve as a basis for empirical treatment of urinary tract infections (utis), as antibiotic-resistance rates may vary geographically with significant differences between countries and regions, and with time.201728621642
genome-wide transposon mutagenesis of proteus mirabilis: essential genes, fitness factors for catheter-associated urinary tract infection, and the impact of polymicrobial infection on fitness requirements.the gram-negative bacterium proteus mirabilis is a leading cause of catheter-associated urinary tract infections (cautis), which are often polymicrobial. numerous prior studies have uncovered virulence factors for p. mirabilis pathogenicity in a murine model of ascending uti, but little is known concerning pathogenesis during cauti or polymicrobial infection. in this study, we utilized five pools of 10,000 transposon mutants each and transposon insertion-site sequencing (tn-seq) to identify the ...201728614382
genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples.proteus mirabilis is an opportunistic pathogen, commonly associated with complicated urinary tract infections (utis). utis caused by multidrug-resistant proteus mirabilis have increased worldwide. multidrug-resistance of gram-negative enteric bacteria is usually associated with class 1 integrons.201728612707
identification and characterization of new members of the sxt/r391 family of integrative and conjugative elements (ices) in proteus mirabilis.integrative and conjugative elements (ices) are self-transmissible chromosomal mobile elements that play significant roles in the dissemination of antimicrobial resistance genes. identification of the structures and functions of ices, particularly those in pathogens, improves understanding of the dissemination of antimicrobial resistance. this study identified new members of the sulfamethoxazole-trimethoprim (sxt)/r391 family of ices that could confer multi-drug resistance in the opportunistic p ...201728602701
in vitro antibacterial activity of poly (amidoamine)-g7 dendrimer.nano-scale dendrimers are synthetic macromolecules that frequently used in medical and health field. traditional anibiotics are induce bacterial resistence so there is an urgent need for novel antibacterial drug invention. in the present study seventh generation poly (amidoamine) (pamam-g7) dendrimer was synthesized and its antibacterial activities were evaluated against representative gram- negative and gram-positive bacteria.201728583153
persistence of blacmy-2-producing proteus mirabilis in two gull colonies at a 1-year interval in southern france. 201728572037
effect of the 2014 clinical and laboratory standards institute urine-specific breakpoints on cefazolin susceptibility rates at a community teaching hospital.enterobacteriaceae, which include escherichia coli, klebsiella pneumoniae, and proteus mirabilis, are identified as the infectious etiology in the majority of urinary tract infections (utis) in community hospitals across the united states. the minimum inhibitory concentration (mic) is a useful tool when choosing an appropriate antibacterial agent. recent changes to the 2014 clinical and laboratory standards institute (clsi) guidelines included reporting a urine-specific cefazolin breakpoint for ...201728558707
dissemination of bla oxa-370 gene among several enterobacteriaceae species in brazil.oxa-370 is a recently described oxa-48 variant that has only been described in a few enterobacter spp. and klebsiella pneumoniae isolates. the purpose of this study is to assess the prevalence of oxa-370-producing isolates in carbapenem-nonsusceptible enterobacteriaceae recovered from 28 hospitals from brazil. real-time pcr was used to determine the presence of bla ndm-1, bla kpc-2, bla vim-type, bla ges-type, bla oxa-48-like, and bla imp-type genes. a total of 4,451 enterobacteriaceae were scre ...201728555403
collaborative research on puerperal infections in bangladesh.bangladesh is considered as a high-risk country for emerging infectious diseases because of its high population density, poverty, and unhygienic conditions. although control efforts have primarily been focused on major infectious diseases such as diarrheal diseases, tuberculosis, malaria, and hiv infection, the prevalence and impact of many local or minor infectious diseases are still unclarified in this country. in this review, we present our recent experience and outcomes of collaborative rese ...201728552890
influence of different wild-garlic (allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties.as there are no previous studies of the european wild-garlic (allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts.201728543032
development and characterization of a stable adhesive bond between a poly(dimethylsiloxane) catheter material and a bacterial biofilm resistant acrylate polymer coating.catheter associated urinary tract infections are the most common health related infections worldwide, contributing significantly to patient morbidity and mortality and increased health care costs. to reduce the incidence of these infections, new materials that resist bacterial biofilm formation are needed. a composite catheter material, consisting of bulk poly(dimethylsiloxane) (pdms) coated with a novel bacterial biofilm resistant polyacrylate [ethylene glycol dicyclopentenyl ether acrylate (eg ...201728535686
environmental monitoring and assessment of antibacterial metabolite producing actinobacteria screened from marine sediments in south coastal regions of karnataka, india.assessment of the therapeutic potential of secondary metabolite producing microorganisms from the marine coastal areas imparts scope and application in the field of environmental monitoring. the present study aims to screen metabolites with antibacterial potential from actionbacteria associated with marine sediments collected from south coastal regions of karnataka, india. the actinobacteria were isolated and characterized from marine sediments by standard protocol. the metabolites were extracte ...201728534309
the link between proteus mirabilis, environmental factors and autoantibodies in rheumatoid arthritis.rheumatoid arthritis (ra) is a relatively common and potentially disabling immune-mediated inflammatory systemic disease, predominantly affecting women and characterised by multiple small joint arthritis. extensive data supports the roles of genetic, environmental and microbial factors in the triggering and development of this disease. proteus mirabilis is considered as the main microbial culprit in the causation of ra. the evidence for the role of these microbes in ra and their links with commo ...201728516867
development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens.to develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (rpa) assays for detection of common bacterial urinary tract infection (uti) pathogens.201728510991
phenotypic detection and antibiogram of β-lactamase-producing proteus species in a tertiary care hospital, india.proteus species cause a variety of community- and hospital-acquired illnesses. synthesis of β-lactamases is the predominant mechanism for resistance to β-lactam antibiotics. among the β-lactamases, extended spectrum β-lactamases (esbls) and ampc β-lactamases are the most common.201728503342
antimicrobial resistance patterns in women with positive urine culture: does menopausal status make a significant difference?aim. urinary tract infection (uti) is considered one of the most common bacterial infections in women. the aim of this study was to investigate the types of uropathogens present, as well as the degree of antimicrobial drug resistance seen among premenopausal (n = 2748) and postmenopausal (n = 1705) women with uncomplicated uti. methods. urinary samples (n = 4453) collected from women with uti were analyzed in terms of uropathogens present. these were considered as positive if bacterial growth wa ...201728497048
characterisation of a thiamine diphosphate-dependent alpha-keto acid decarboxylase from proteus mirabilis jn458.alpha-keto acid decarboxylases can convert keto acids to their corresponding aldehydes, which are often volatile aroma compounds. the gene encoding α-keto acid decarboxylase in proteus mirabilis jn458 was cloned, and the enzyme overexpressed in escherichia coli bl21 (de3), purified in high yield, and characterised. the molecular weight is 62.291kda by maldi-tof ms, and optimum activity at ph 6.0 and 40-50°c. the enzyme is a typical decarboxylase, dependent on thiamine diphosphate and mg(2+) as c ...201728490063
synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against gram-positive and gram-negative bacteria.reduced graphene oxide (rgo) is a promising antibacterial material, the efficacy of which can be further enhanced by the addition of silver nanoparticles (nag). in this study, the mechanisms of antibacterial activity of rgo-nag nanocomposite against several important human pathogenic multi-drug resistant bacteria, namely gram-positive coccal staphylococcus aureus and gram-negative rod-shaped escherichia coli and proteus mirabilis are investigated. at the same concentration (100 µg/ml), rgo-nag n ...201728484209
fourier transform infrared spectroscopy as a tool in analysis of proteus mirabilis endotoxins.fourier transform infrared spectroscopy (ft-ir) was used to scan whole bacterial cells as well as lipopolysaccharides (lpss, endotoxins) isolated from them. proteus mirabilis cells, with chemically defined lpss, served as a model for the atr ft-ir method. the paper focuses on three steps of infrared spectroscopy: (1) sample preparation, (2) ir scanning, and (3) multivariate analysis of ir data (principal component analysis, pca).201728478562
assessment of urinary pharmacokinetics and pharmacodynamics of orbifloxacin in healthy dogs with ex vivo modelling.the aim of this study was to investigate the urinary pharmacokinetics (pk) of orbifloxacin (obfx) administered at 5 mg kg-1 in six healthy dogs. a further aim was to use an ex vivo model to evaluate the urinary pk and pharmacodynamics (pd) of obfx to determine its urinary bactericidal titre (ubt), which represents the maximal dilution of urine allowing bactericidal activity.201728470147
the impact of production of extended-spectrum β-lactamases on the 28-day mortality rate of patients with proteus mirabilis bacteremia in korea.the incidence of proteus mirabilis antimicrobial resistance, especially that mediated by extended-spectrum β-lactamases (esbls), has increased. we investigated the impact of esbl production on the mortality of patients with p. mirabilis bacteremia in korea.201728468622
[effects of vacuum sealing drainage technique in acute and chronic suppurative tenosynovitis of hand].objective: to investigate the effects of vacuum sealing drainage technique in acute and chronic suppurative tenosynovitis of hand. methods: a total of 9 cases acute and chronic suppurative tenosynovitis patients from january 2013 to april 2015 in puai hospital affiliated to tongji medical college, huazhong university of science and technology were retrospectively reviewed. there were 6 males and 3 females, aging from 27 to 65 years, the average age was 55 years. there were 3 cases of index finge ...201728464581
genome sequence of a providencia stuartii strain isolated from luciliasericata salivary glands.we present here the draft genome sequence of a providencia stuartii strain, derived from the salivary glands of larval lucilia sericata, a common blow fly important to forensic, medical, and veterinary science. the genome sequence will help dissect coinfections involving p. stuartii and proteus mirabilis, as well as blow fly-bacteria interactions.201728450516
recurrence of deep surgical site infection in cerebral palsy after spinal fusion is rare.retrospective review of prospective registry.201728449964
short ftsz filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis.ftsz, the bacterial homologue of eukaryotic tubulin, plays a central role in cell division in nearly all bacteria and many archaea. it forms filaments under the cytoplasmic membrane at the division site where, together with other proteins it recruits, it drives peptidoglycan synthesis and constricts the cell. despite extensive study, the arrangement of ftsz filaments and their role in division continue to be debated. here, we apply electron cryotomography to image the native structure of intact ...201728438890
multidrug resistance salmonella genomic island 1 in a morganella morganii subsp. morganii human clinical isolate from france.salmonella genomic island 1 (sgi1) is a multidrug resistance integrative mobilizable element that harbors a great diversity of antimicrobial resistance gene clusters described in numerous salmonella enterica serovars and also in proteus mirabilis. a serious threat to public health was revealed in the recent description in p. mirabilis of a sgi1-derivative multidrug resistance island named pgi1 (proteus genomic island 1) carrying extended-spectrum-β-lactamase (esbl) and metallo-β-lactamase resist ...201728435889
superficial swab versus deep-tissue biopsy for the microbiological diagnosis of local infection in advanced-stage pressure ulcers of spinal-cord-injured patients: a prospective study.to assess the predictive value of superficial ulcer swab culture to make a microbiological diagnosis of deep wound infections in spinal cord injury (sci) patients with advanced-stage pressure ulcers.201728433727
formulation of carbapenems loaded gold nanoparticles to combat multi-antibiotic bacterial resistance: in vitro antibacterial study.despite the fact that carbapenems (powerful β-lactams antibiotics) were able to fight serious infectious diseases, nowadays the spread of carbapenems-resistant bacteria is considered the main challenge in antibacterial therapy. in this study, we focused on evaluating the surface conjugation of carbapenems (imipenem and meropenem) with gold nanoparticles as a delivering strategy to specifically and safely maximize their therapeutic efficacy while destroying the developing resistance of the pathog ...201728411141
bacterial epidemiology and antimicrobial resistance profiles of urinary specimens of the elderly.although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles.201728408060
antibacterial effects of lactobacillus isolates of curd and human milk origin against food-borne and human pathogens.this study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific pcr and 16s rrna-based sequencing. these strains belonged to five species, lactobacillus casei, l. delbrueckii, l. fermentum, l. plantarum, and l. pentosus. antibacterial activities of cell-free supernatants (cfss) of all the lactobacillus isolates were estim ...201728401466
in vitro activity of fosfomycin trometamol and other oral antibiotics against multi-drug-resistant uropathogens.clinical midstream and urinary catheter isolates (n=106) of extended-spectrum β-lactamase (esbl)-positive escherichia coli, carbapenemase-producing klebsiella pneumoniae, proteus mirabilis and meticillin-resistant staphylococcus saprophyticus were tested against fosfomycin using the agar dilution method, the broth microdilution method and the gradient test described by the clinical and laboratory standards institute. nitrofurantoin, co-trimoxazole, amoxicillin/clavulanic acid, cefuroxime, levofl ...201728390962
complete genome sequence of proteus mirabilis phage ppm_01 isolated from raw sewage.phage ppm_01 was previously isolated from a raw sewage treatment facility located in batu maung, penang, malaysia, and it was highly lytic against proteus mirabilis, which causes urinary tract infections in humans. in this paper, we characterize the biology and complete genome sequence of the phage.201728384626
antimicrobial susceptibility monitoring of bacterial pathogens isolated from urinary tract infections in dogs and cats across europe: compath results.compath is a pan-european antimicrobial surveillance program collecting bacterial pathogens from dogs and cats not recently exposed to antimicrobials. we present minimum inhibitory concentration data obtained using clinical and laboratory standards institute methodology for 616 urinary tract infection (uti) isolates collected between 2008 and 2010. in both dogs and cats, the most common pathogen was escherichia coli (59.8% and 46.7%, respectively). antimicrobial activity against e. coli in dogs ...201728384093
[community acquired urinary tract infections in older adults].background community acquired urinary tract infections (cauti) in adults are common ailments. older adults are prone to infectious diseases, diagnosis can be difficult, their etiologic and antimicrobial resistance are poorly known. objectives to evaluate the incidence discriminated by sex, symptoms, and to determine pathogens and their antimicrobial resistance rate. methods a retrospective, descriptive and comparative study. we analyze uricultures among 2013 in the hospital nacional de clinicas ...201728379126
[expression of proteus mirabilis polyphosphate kinase and preparation of its polyclonal antibodies].to express and purify polyphosphate kinase (ppk) from proteus mirabilis and prepare the polyclonal antibody against ppk.201728377345
antimicrobial activity and chemical composition of "kpètè-kpètè": a starter of benin traditional beer tchoukoutou.the aim of this study was to investigate the antibacterial effect of the crude starter "kpètè-kpètè" and lactic acid bacteria used during the production of "tchoukoutou." to achieve this, a total of 11 lactic acid bacteria and 40 starter samples were collected from four communes. the samples were tested on 29 gram + and - strains by disk diffusion method. the minimum inhibitory and bactericidal concentrations of starter and lactic acid bacteria were determined by conventional methods. organic ac ...201728367445
n-heterocyclic choline analogues based on 1,2,3,4-tetrahydro(iso)quinoline scaffold with anticancer and anti-infective dual action.pharmacological effects of biologically active "small molecules" can be improved by their targeted modification, which affects drug delivery and interaction with tumor cells and microorganisms. we aimed to evaluate anticancer and antimicrobial activity of lipid-like choline derivatives modified via simultaneous introduction of tetrahydro(iso)quinoline based pharmacophore system at nitrogen atom and long chain alkyl substituent at oxygen atom.201728364698
first report in africa of two clinical isolates of proteus mirabilis carrying salmonella genomic island (sgi1) variants, sgi1-pmabb and sgi1-w.two proteus mirabilis strains, designated pmtan59 and pmkaf126, were isolated from two different egyptian cities in 2014 and 2015, respectively. pmtan59 was isolated from a sputum swab from a pneumonia patient in tanta university teaching hospital. pmkaf126 was isolated from a patient with a diabetic foot infection in a hospital in the city of kafr el-sheikh. the two isolates were identified with bacterial small ribosomal rna (16s rrna) gene amplification and sequencing and tested for antimicrob ...201728359833
bioactivity of variant molecular weight chitosan against drug-resistant bacteria isolated from human wounds.chitosan available from crab shells is usually of high molecular weight which may result in reduced efficiency for its antibacterial activity. one of the techniques for improving chitosan antibacterial efficiency is reducing its molecular weight. the irradiation of chitosan by gamma radiations is considered to be one of the most effective and widely used methods for improving its antibacterial activity. chitosan obtained from crab shells was irradiated with gamma radiations at different doses, a ...201728358617
antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago.twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ...201628352539
veb-1 extended-spectrum β-lactamase-producing multidrug-resistant proteus mirabilis sepsis outbreak in a neonatal intensive care unit in india: clinical and diagnostic implications.extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae, multidrug-resistant (mdr) pathogens, are increasingly implicated in nosocomial outbreaksworldwide, particularly in neonatal intensive care units (nicus). proteus mirabilis is an uncommon nosocomial pathogen causing sepsis in neonates.201628348778
screening, production, optimization and characterization of β-glucosidase using microbes from shellfish waste.an extracellular β-glucosidase was isolated from proteus mirabilis vit117 found to be growing on prawn shells. the enzyme production was found to be enhanced (14.58 u/ml) when the culture was maintained at ph 9 and provided with sorbitol as carbon source, yeast extract as nitrogen source and incubated at 37 °c for approximately 72 h. statistical methods like plackett-burman and rsm were also applied here to study the effects of different combinations of growth parameters for the bacteria, where ...201628330285
isolation and characterization of plant and human pathogenic bacteria from green pepper (capsicum annum l.) in riyadh, saudi arabia.forty-three bacterial isolates in five genera were recovered from naturally infected green pepper fruits (38 samples) showing dark brown, irregular-shaped splotches. the pathogenicity test was performed on healthy green pepper fruits and red colonies were from inoculated fruits showing the same symptoms and the infected area developed into soft rot. their identification was based on phenotypic characters and sequence of the gene fragment coding 16s rrna. of 43 isolates, 10 showing splotches on g ...201428324470
characterization of the genetic environment of the blaveb-4 gene, associated with a transposable region in a proteus mirabilis clinical isolate.proteus mirabilis is the second most common cause of urinary tract infections and is also an important cause of nosocomial infections. tem-type and ctx-m-type extended-spectrum β-lactamases (esbls) are the most widely distributed in this bacterial species, but minor esbls such as the veb-type have also been identified. the aim of this study was to analyze the genetic environment of the blaveb-4 gene found in a p. mirabilis clinical isolate recovered in spain. p. mirabilis n2231 showed resistance ...201728304213
the effect of urinary catheters on microbial biofilms and catheter associated urinary tract infections.the aims of this study were to determine relationship between biofilm producer microorganisms attached to urinary catheters (ucs) and urinary catheter-associated urinary tract infections (cautis), to determine the rate of cauti development and the relationship between cauti and catheterization period in catheterized patients.201728299769
in vitro activity of imipenem against carbapenemase-positive enterobacteriaceae: smart global surveillance program 2008-2014.the study for monitoring antimicrobial resistance trends (smart) global surveillance program collected 103,960 isolates of enterobacteriaceae from 2008 to 2014. from this isolate collection all ertapenem-non-susceptible isolates (mic, ≥1 μg/ml; n = 3,428) and 9,371 isolates of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, and proteus mirabilis with an ertapenem-susceptible extended-spectrum β-lactamase (esbl)-positive phenotype were assessed for the presence of common carbapenemas ...201728298454
proteolysis of truncated hemolysin a yields a stable dimerization interface.wild-type and variant forms of hpma265 (truncated hemolysin a) from proteus mirabilis reveal a right-handed, parallel β-helix capped and flanked by segments of antiparallel β-strands. the low-salt crystal structures form a dimeric structure via the implementation of on-edge main-chain hydrogen bonds donated by residues 243-263 of adjacent monomers. surprisingly, in the high-salt structures of two variants, y134a and q125a-y134a, a new dimeric interface is formed via main-chain hydrogen bonds don ...201728291749
fosfomycin and comparator activity against select enterobacteriaceae, pseudomonas, and enterococcus urinary tract infection isolates from the united states in 2012.fosfomycin is a broad-spectrum cell wall active agent that inhibits the mura enzyme involved in peptidoglycan synthesis and is fda-approved for treatment of uncomplicated urinary tract infections (utis) caused by escherichia coli and enterococcus faecalis in women. data regarding the susceptibility of recent uti isolates to fosfomycin are limited.201728285420
prevalence of extended-spectrum beta-lactamases-producing microorganisms in patients admitted at krrh, southwestern uganda.the emergence of extended-spectrum beta-lactamase- (esbl-) producing pathogenic bacteria at kabale regional referral hospital (krrh), located in southwestern uganda, is of great concern: a phenomenon that worries clinicians and other healthcare workers due to the serious threat they pose to patients. this current study aimed at determining the phenotypic detection of esbl-producing strains of e. coli, klebsiella sp., and proteus sp. isolated from clinical specimens and their prevalence in patien ...201728270849
antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli isolated from chinese patients with urinary tract infections between 2010 and 2014.the objective of this study was to investigate the distribution and susceptibility of aerobic and facultative gram-negative bacilli isolated from chinese patients with utis collected within 48 h (community acquired, ca) or after 48 h (hospital acquired, ha) of hospital admission.201728264656
in vitro activity of ceftaroline and comparator agents against gram-positive and gram-negative clinical isolates from cancer patients.bacterial infections are common in cancer patients. ceftaroline (cft) is a broad-spectrum cephalosporin with activity against most gram-positive organisms (gpos) and many gram-negative organisms. in this study, the in vitro activity of cft was compared with vancomycin (van), daptomycin (dap), linezolid (lzd), trimethoprim/sulphamethoxazole (sxt) and tigecycline (tig) against bacteria (predominantly blood culture isolates) isolated from cancer patients in 2014 and 2015. cft was active against met ...201728257904
very high prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae in bacteriemic patients hospitalized in teaching hospitals in bamako, mali.the worldwide dissemination of extended-spectrum beta-lactamase producing enterobacteriaceae, (esbl-e) and their subset producing carbapenemases (cpe), is alarming. limited data on the prevalence of such strains in infections from patients from sub-saharan africa are currently available. we determined, here, the prevalence of esbl-e/cpe in bacteriemic patients in two teaching hospitals from bamako (mali), which are at the top of the health care pyramid in the country. during one year, all entero ...201728245252
necrotizing suppurative nephritis in a japanese black feedlot steer due to proteus mirabilis infection.a japanese black feedlot steer suddenly died after exhibiting astasia and cramping of the extremities. necropsy of the animal revealed that the right kidney was enlarged and pale with severe nephrolithiasis. the urinary bladder displayed mucosal hemorrhage. upon bacteriological investigation, proteus mirabilis was isolated from the liver, spleen, right kidney, lungs and urine. histopathological examination revealed necrotizing suppurative nephritis with the presence of numerous gram-negative bac ...201728239050
chromosomal location of the fosa3 and blactx-m genes in proteus mirabilis and clonal spread of escherichia coli st117 carrying fosa3-positive inchi2/st3 or f2:a-:b- plasmids in a chicken farm.the aim of this study was to investigate the spread and location of the fosa3 gene among enterobacteriaceae from diseased broiler chickens. twenty-nine escherichia coli and seven proteus mirabilis isolates recovered from one chicken farm were screened for the presence of plasmid-mediated fosfomycin resistance genes by pcr. the clonal relatedness of fosa3-positive isolates, the transferability and location of fosa3, and the genetic context of the fosa3 gene were determined. seven p. mirabilis iso ...201728238801
determination of antibacterial and technological properties of vaginal lactobacilli for their potential application in dairy products.functional foods could differently affect human health in relation to the gender. recent studies have highlighted the anti-candida and anti-chlamydia activities of some lactobacillus strains isolated from the vagina of healthy women. considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. in this context, the aim of this work was to study some f ...201728223974
biofilm formation and immunomodulatory activity of proteus mirabilis clinically isolated strains.urinary tract infections (utis) and catheter-associated utis (cautis) are the principal hospital-acquired infections. proteus mirabilis is characterized by several virulence factors able to promote adhesion and biofilm formation and ameliorate the colonization of urinary tract and the formation of crystalline biofilms on the abiotic surface of the urinary catheters. since, to date, the role of p. mirabilis in the etiopathogenesis of different types of urinary tract infections is not well establi ...201728212280
potassium iodide potentiates broad-spectrum antimicrobial photodynamic inactivation using photofrin.it is known that noncationic porphyrins such as photofrin (pf) are effective in mediating antimicrobial photodynamic inactivation (apdi) of gram-positive bacteria or fungi. however, the apdi activity of pf against gram-negative bacteria is accepted to be extremely low. here we report that the nontoxic inorganic salt potassium iodide (ki) at a concentration of 100 mm when added to microbial cells (10(8)/ml) + pf (10 μm hematoporphyrin equivalent) + 415 nm light (10 j/cm(2)) can eradicate (>6 log ...201728207234
inhibition of urease enzyme production and some other virulence factors expression in proteus mirabilis by n-acetyl cysteine and dipropyl disulphide.proteus mirabilis is one of the important pathogens that colonize the urinary tract and catheters resulting in various complications, such as blockage of the catheters and the formation of infective stones.201728190143
antibacterial activities of manuka and honeydew honey-based membranes against bacteria that cause wound infections in animals.in this study, membranes composed of honey (manuka or honeydew) and pectin were developed, and the iso 22196 method was used to evaluate their antibacterial activities against multidrug-resistant bacteria (i.e., staphylococcus pseudointermedius, escherichia coli, proteus mirabilis, pseudomonas aeruginosa) that cause wound infection in animals. the results demonstrated that both manuka and honeydew honey-based membranes had strong antibacterial activities against the strain of methicillin-resista ...201728174146
molecular epidemiology of carbapenemase-producing enterobacteriaceae in a primary care hospital in japan, 2010-2013.recently, carbapenemase-producing enterobacteriaceae (cpe) have been spreading worldwide and have become a threat in healthcare systems. we investigated the isolation frequency and molecular epidemiological characteristics of cpe isolated from clinical samples collected at a primary care hospital over the four years of 2010-2013 in japan. cpe were detected in 17 (0.34%) of 4875 isolates by the broth microdilution method, sodium mercaptoacetate inhibition test, and modified hodge test using merop ...201728161293
detection of sgi1/pgi1 elements and resistance to extended-spectrum cephalosporins in proteae of animal origin in france.proteae, and especially proteus mirabilis, are often the cause of urinary tract infections (utis) in humans. they were reported as carriers of extended-spectrum β-lactamase (esbl) genes, and recently of carbapenemases, mostly carried by the salmonella genomic island 1 (sgi1) and proteus genomic island 1 (pgi1). proteae have also lately become an increasing cause of utis in companion animals, but antimicrobial susceptibility data in animals are still scarce. here, we report the characterization o ...201728154560
antimicrobial activity study of triclosan-loaded wbpu on proteus mirabilis in vitro.to evaluate the antimicrobial activity study of triclosan-loaded waterborne polyurethanes (wbpu) on proteus mirabilis in vitro.201728150092
potency and selectivity indices of acetone leaf extracts of nine selected south african trees against six opportunistic enterobacteriaceae isolates from commercial chicken eggs.the rise in antimicrobial resistance in a plethora of nosocomial and opportunistic bacterial pathogens often isolated from commercial eggs, poses a serious public health concern. the existence of these contaminants may also serve as a drawback in the current efforts of improving the well-being of immunocompromised patients. the aim of this study was to determine the efficacy of plant extracts that had good activity on escherichia coli in previous word on pathogens isolated from eggs for possible ...201728148263
antimicrobial activity, ame resistance, and a-site binding studies of anthraquinone-neomycin conjugates.the antibacterial effects of aminoglycosides are based on their association with the a-site of bacterial rrna and interference with the translational process in the bacterial cell, causing cell death. the clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12s rrna and its deafness-associated mutations, c1494u and a1555g. we report a rapid assay that allows screening of aminoglycoside compounds to ...201728103015
dissemination of blaoxa-58 in proteus mirabilis isolates from germany.characterization of proteus mirabilis isolates harbouring blaoxa-58 with emphasis on the genetic environment of this resistance determinant.201728093482
hatchery workers' igg antibody profiles to airborne bacteria.occupational exposure to high concentrations of airborne bacteria in poultry production is related to an increased risk of respiratory disorders. however, etiology and in particular microorganisms' potential role in pathogenesis still needs to be elucidated. thus, detection of specific antibodies against occupational microbial antigens may lead to identification of potentially harmful species. for the purpose of igg titer determination, indirect immunofluorescence on various bacterial isolates f ...201728089178
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