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an in vitro synthetic biosystem based on acetate for production of phloroglucinol.phloroglucinol is an important chemical, and the biosynthesis processes which can convert glucose to phloroglucinol have been established. however, due to approximate 80% of the glucose being transformed into undesirable by-products and biomass, this biosynthesis process only shows a low yield with the highest value of about 0.20 g/g. the industrial applications are usually hindered by the low current productivity and yield and also by the high costs. generally, several different aspects limit t ...201728789688
erratum for ho et al., "complete genome sequence of acinetobacter calcoaceticus ca16, a bacterium capable of degrading diesel and lignin". 201728774994
structure guided design of protein biosensors for phenolic pollutants.phenolic aromatic compounds are a major source of environmental pollution. currently there are no in situ methods for specifically and selectively detecting these pollutants. here, we exploit the nature's biosensory machinery by employing acinetobacter calcoaceticus ncib8250 protein, mopr, as a model system to develop biosensors for selective detection of a spectrum of these pollutants. the x-ray structure of the sensor domain of mopr was used as a scaffold for logic-based tunable biosensor desi ...201728723202
first report of cavitary pneumonia due to community-acquired acinetobacter pittii, study of virulence and overview of pathogenesis and treatment.acinetobacter pittii is a nosocomial pathogen rarely involved in community-acquired infections. we report for the first time that a. pittii can be responsible for cavitary community-acquired pneumonia and study its virulence, and discuss its pathogenesis and treatment options.201728683724
complete genome sequence of acinetobacter calcoaceticus ca16, a bacterium capable of degrading diesel and lignin.we report here the complete assembled genome sequence of acinetobacter calcoaceticus ca16, which is capable of utilizing diesel and lignin as a sole carbon source. ca16 contains a 4,110,074-bp chromosome and a 5,920-bp plasmid. the assembled sequences will help elucidate potential metabolic pathways and mechanisms responsible for ca16's hydrocarbon degradation ability.201728619790
characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation.bioremediation of polluted soils is a promising technique with low environmental impact, which uses soil organisms to degrade soil contaminants. in this study, 19 bacterial strains isolated from a diesel-contaminated soil were screened for diesel-degrading potential, biosurfactant production, and biofilm formation abilities, all desirable characteristics when selecting strains for re-inoculation into hydrocarbon-contaminated soils. diesel degradation rates were determined in vitro in minimal med ...201728598213
development and evaluation of a core genome multilocus typing scheme for whole-genome sequence-based typing of acinetobacter baumannii.we have employed whole genome sequencing to define and evaluate a core genome multilocus sequence typing (cgmlst) scheme for acinetobacter baumannii. to define a core genome we downloaded a total of 1,573 putative a. baumannii genomes from ncbi as well as representative isolates belonging to the eight previously described international a. baumannii clonal lineages. the core genome was then employed against a total of fifty-three carbapenem-resistant a. baumannii isolates that were previously typ ...201728594944
carbapenem-resistant acinetobacter baumannii contamination in an intensive care unit.acinetobacter baumannii is a major pathogen causing infections in intensive care units (icus). in this study, we aimed to evaluate the presence of a. baumannii in an icu environment and gloves from icu workers and to characterize the antimicrobial resistance of the isolates in comparison with those isolated from icu patients at the same hospital.201728562751
epidemiology and trends in the antibiotic susceptibilities of gram-negative bacilli isolated from patients with intra-abdominal infections in the asia-pacific region, 2010-2013.this study was conducted to investigate the epidemiology and antimicrobial susceptibility patterns of gram-negative bacilli (gnb) isolated from intra-abdominal infections (iais) in the asia-pacific region (apr) from 2010-2013. a total of 17 350 isolates were collected from 54 centres in 13 countries in the apr. the three most commonly isolated gnb were escherichia coli (46.1%), klebsiella pneumoniae (19.3%) and pseudomonas aeruginosa (9.8%). overall, the rates of extended-spectrum β-lactamase (e ...201728435019
complete genome sequence and methylome analysis of acinetobacter calcoaceticus 65.acinetobacter calcoaceticus 65 is the original source strain for the restriction enzyme acc65i. its complete sequence and full methylome were determined using single-molecule real-time (smrt) sequencing.201728336599
novel oxadiazole thioglycosides as potential anti-acinetobacter agents.the glycosylation of 1,3,4-oxadiazole-2-thiones has been performed with peracetylated β-pyranosyl bromide in the presence of potassium carbonate. deprotection of acetylated thioglycosides was necessary for increasing their antibacterial effects. the structures of nucleosides were confirmed by (1)h nmr, (13)c nmr and hrms. the anomeric protons of nucleosides c1-4 were assigned to the doublet, confirming the β-configuration. the synthesized compounds were tested for their antimicrobial activity ag ...201628243273
lettuce and fruits as a source of multidrug resistant acinetobacter spp.the role of ready-to-eat products as a reservoir of pathogenic species of acinetobacter remains unclear. the objective of the present study was to evaluate the presence of acinetobacter species in lettuces and fruits marketed in portugal, and their susceptibility to antimicrobials. acinetobacter spp. were isolated from 77.9% of the samples and these microorganisms were also found as endophytes (i.e. present within the plant tissue) in 12 of 20 samples of lettuces analysed. among 253 isolates tha ...201728213015
effects of meropenem exposure in persister cells of acinetobacter calcoaceticus-baumannii.to evaluate the influence of meropenem in the acinetobacter calcoaceticus-baumannii (acb) persister levels.201728111984
clinical and microbiological characteristics of bloodstream infections among patients with haematological malignancies with and without neutropenia at a medical centre in northern taiwan, 2008-2013.this study aimed to investigate the epidemiology and microbiology of bloodstream infections (bsis) in patients with haematological malignancies. clinical characteristics and microbiology of 2083 patients with haematological malignancy who were treated for bsi from 2008 to 2013 at a medical centre in taiwan were retrospectively reviewed. lymphoma (38.1%) was the most common, followed by acute myeloid leukaemia (30.9%). of the 2090 non-duplicate bsi isolates, 1310 (62.7%) were recovered from patie ...201728109554
draft genome sequence of jvap01t, the type strain of the novel species acinetobacter dijkshoorniae.here, we report the draft genome sequence of the type strain of acinetobacter dijkshoorniae, a novel human pathogen within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex. strain jvap01(t) has an estimated genome size of 3.9 mb, exhibits a 38.8% g+c content, and carries a plasmid with the blandm-1 carbapenemase gene.201728082506
clinical and pathophysiological overview of acinetobacter infections: a century of challenges.summaryacinetobacter is a complex genus, and historically, there has been confusion about the existence of multiple species. the species commonly cause nosocomial infections, predominantly aspiration pneumonia and catheter-associated bacteremia, but can also cause soft tissue and urinary tract infections. community-acquired infections by acinetobacter spp. are increasingly reported. transmission of acinetobacter and subsequent disease is facilitated by the organism's environmental tenacity, resi ...201727974412
engineering cyclohexanone monooxygenase for the production of methyl propanoate.a previous study showed that cyclohexanone monooxygenase from acinetobacter calcoaceticus (acchmo) catalyzes the baeyer-villiger oxidation of 2-butanone, yielding ethyl acetate and methyl propanoate as products. methyl propanoate is of industrial interest as a precursor of acrylic plastic. here, various residues near the substrate and nadp(+) binding sites in acchmo were subjected to saturation mutagenesis to enhance both the activity on 2-butanone and the regioselectivity toward methyl propanoa ...201727935281
evaluation of an ultraviolet c light-emitting device for disinfection of electronic devices.a tabletop-type ultraviolet c (uvc) light-emitting disinfecting device was evaluated for microbiologic effectiveness, safety, usability, and end-user satisfaction.201627742144
emergence of new delhi metallo-beta-lactamase 1 and other carbapenemase-producing acinetobacter calcoaceticus-baumannii complex among patients in hospitals in ha noi, viet nam.acinetobacter baumannii is an important cause of multidrug-resistant hospital acquired infections in the world. here, we investigate the presence of ndm-1 and other carbapenemases among carbapenem-resistant a. baumannii isolated between august 2010 and december 2014 from three large hospitals in hanoi, vietnam. we identified 23/582 isolates (4 %) (11 from hospital a, five from hospital b, and seven from hospital c) that were ndm-1 positive, and among them 18 carried additional carbapenemase gene ...201727714593
multi-drug-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex infection outbreak in dogs and cats in a veterinary hospital.members of the acinetobacter calcoaceticus-acinetobacter baumannii complex cause severe outbreaks in humans, and are increasingly reported in animals.201627709647
opportunistic infections in hiv-infected patients differ strongly in frequencies and spectra between patients with low cd4+ cell counts examined postmortem and compensated patients examined antemortem irrespective of the haart era.aids-related mortality has changed dramatically with the onset of highly active antiretroviral therapy (haart), which has even allowed compensated hiv-infected patients to withdraw from secondary therapy directed against opportunistic pathogens. however, in recently autopsied hiv-infected patients, we observed that associations with a broad spectrum of pathogens remain, although detailed analyses are lacking. therefore, we focused on the possible frequency and spectrum shifts in pathogens associ ...201627611681
pcr assay based on the gyrb gene for rapid identification of acinetobacter baumannii-calcoaceticus complex at specie level.the genus acinetobacter sp. comprises more than 50 species, and four are closely related and difficult to be distinguished by either phenotypic or genotypic methods: the acinetobacter calcoaceticus-baumannii complex (abc). the correct identification at species level is necessary mainly due to the epidemiological aspects.201627605498
the role of the genetic elements bla oxa and is aba 1 in the acinetobacter calcoaceticus-acinetobacter baumannii complex in carbapenem resistance in the hospital setting.members of the acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. oxacillinases are primarily responsible for resistance to carbapenem antibiotics. higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the isaba1 sequence, near bla oxa genes. the present study examined the occurrence of the genetic elements bla oxa and isaba1 and their relationship with susc ...201727598629
tigecycline-based versus sulbactam-based treatment for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex.the treatment options for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii (mdr acb) complex are limited, and the optimal treatment has not been established.201627496018
characteristics of invasive acinetobacter species isolates recovered in a pediatric academic center.acinetobacter species are associated with increasing mortality due to emerging drug-resistance. pediatric acinetobacter infections are largely undefined in developed countries and clinical laboratory identification methods do not reliably differentiate between members of the acinetobacter calcoaceticus-baumannii complex, leading to improper identification. therefore we aimed to determine risk factors for invasive acinetobacter infections within an academic, pediatric setting as well as defining ...201627449800
acinetobacter dijkshoorniae sp. nov., a member of the acinetobacter calcoaceticus-acinetobacter baumannii complex mainly recovered from clinical samples in different countries.the recent advances in bacterial species identification methods have led to the rapid taxonomic diversification of the genus acinetobacter. in the present study, phenotypic and molecular methods have been used to determine the taxonomic position of a group of 12 genotypically distinct strains belonging to the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex, initially described by gerner-smidt and tjernberg in 1993, that are closely related to acinetobacter pittii. strains chara ...201627432448
rapid species identification and epidemiological analysis of carbapenem-resistant acinetobacter spp. by a pcr-based open reading frame typing method.the spread of carbapenem-resistant acinetobacter spp. has become a global problem. in this study, 18 carbapenem-resistant acinetobacter calcoaceticus-baumannii (acb) complexes, identified using a conventional biochemical method at our hospital during 2004-2013, were studied for species identification and epidemiological analyses. species identification was performed using matrix-assisted laser desorption ionization-time-of-flight ms, a partial sequence analysis of rpob and a pcr-based orf typing ...201627412356
structural basis of selective aromatic pollutant sensing by the effector binding domain of mopr, an ntrc family transcriptional regulator.phenol and its derivatives are common pollutants that are present in industrial discharge and are major xenobiotics that lead to water pollution. to monitor as well as improve water quality, attempts have been made in the past to engineer bacterial in vivo biosensors. however, due to the paucity of structural information, there is insufficiency in gauging the factors that lead to high sensitivity and selectivity, thereby impeding development. here, we present the crystal structure of the sensor ...201627362503
incidence, management, and visual outcomes in pediatric endophthalmitis following cataract surgery by a single surgeon.to report the incidence of endophthalmitis following cataract surgery by a single surgeon in children and to describe its microbiological profile, management, and visual outcomes.201627343836
development and evaluation of species-specific pcr for detection of nine acinetobacter species.molecular methods have the potential to improve the speed and accuracy of acinetobacter species identification in clinical settings. the goal of this study is to develop species-specific pcr assays based on differences in the rna polymerase beta-subunit gene (rpob) to detect nine commonly isolated acinetobacter species including acinetobacter baumannii, acinetobacter calcoaceticus, acinetobacter pittii, acinetobacter nosocomialis, acinetobacter lwoffii, acinetobacter ursingii, acinetobacter bere ...201627312551
effect of plant growth-promoting bacteria on the growth and fructan production of agave americana l.the effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in agave americana was assessed. the bacterial strains aco-34a, aco-40, and aco-140, isolated from the a. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. the three bacterial strains were evaluated via plant inoculation assays, and azospirillum brasilense cd served as a control strain. phylogenetic analysis based on the 16s rrna gene showe ...201627268113
molecular epidemiology and clinical impact of acinetobacter calcoaceticus-baumannii complex in a belgian burn wound center.multidrug resistant acinetobacter baumannii and its closely related species a. pittii and a. nosocomialis, all members of the acinetobacter calcoaceticus-baumannii (acb) complex, are a major cause of hospital acquired infection. in the burn wound center of the queen astrid military hospital in brussels, 48 patients were colonized or infected with acb complex over a 52-month period. we report the molecular epidemiology of these organisms, their clinical impact and infection control measures taken ...201627223476
the effects of sodium hypochlorite against selected drinking water-isolated bacteria in planktonic and sessile states. 201627156214
isolation and characterization of fipronil degrading acinetobacter calcoaceticus and acinetobacter oleivorans from rhizospheric zone of zea mays.an enrichment culture technique was used for the isolation of bacteria capable of utilizing fipronil as a sole source of carbon and energy. based on morphological, biochemical characteristics and phylogenetic analysis of 16s rrna sequence, the bacterial strains were identified as acinetobacter calcoaceticus and acinetobacter oleivorans. biodegradation experiments were conducted in loamy sand soil samples fortified with fipronil (50 µg kg(-1)) and inoculated with acinetobacter sp. cells (45 × 10( ...201627084098
antibiotic resistance profiles and genetic similarities within a new generation of carbapenem-resistant acinetobacter calcoaceticus-a. baumannii complex resistotypes in bosnia and herzegovina.acinetobacter calcoaceticus-a. baumannii complex (acb complex) is a nosocomial pathogen. due to its high ability to develop antibiotic resistance, it has become a problematic challenge in the modern healthcare system. the molecular and genetic mechanisms of gaining multidrug resistance in acb complex are well known. this study focuses on providing an overview of the antibiotic resistance profiles, genetic similarities and resistotypes, and general characteristics of carbapenem-resistant acb comp ...201627082787
biodegradation of phenol by bacteria strain acinetobacter calcoaceticus pa isolated from phenolic wastewater.a phenol-degrading bacterium strain pa was successfully isolated from the effluent of petrochemical wastewater. based on its morphological, physiological and biochemical characteristics, the strain pa was characterized as a gram-negative, strictly aerobic, nonmotile and short rod-shaped bacterium that utilizes phenol as a sole carbon and energy source. 16s rdna sequence analysis revealed that this strain is affiliated to acinetobacter calcoaceticus in the group of gammaproteobacteria. the strain ...201627005648
blood stream infections caused by acinetobacter baumannii group in japan - epidemiological and clinical investigation.acinetobacter calcoaceticus-acinetobacter baumannii complex, especially a. baumannii, acinetobacter pittii and acinetobacter nosocomialis, constitutes an important group of nosocomial pathogens; however, epidemiological or clinical characteristics and prognosis is limited in japan. from 2009 to 2013, 47 blood stream infection cases resulting from a. baumannii group were reviewed at the national defense medical college, an 800-bed tertiary hospital. to determine the genospecies, further comparati ...201626993173
acinetobacter seifertii isolated from china: genomic sequence and molecular epidemiology analyses.clinical infections caused by acinetobacter spp. have increasing public health concerns because of their global occurrence and ability to acquire multidrug resistance. acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex encompasses a. calcoaceticus, a. baumannii, a. pittii (formerly genomic species 3), and a nosocomial (formerly genomic species 13tu), which are predominantly responsible for clinical pathogenesis in the acinetobacter genus. in our previous study, a putative novel sp ...201626945401
intensive care unit-acquired infections in a tertiary care hospital: an epidemiologic survey and influence on patient outcomes.nosocomial infections are common in intensive care units (icus), but the pattern of infections and the distribution of microorganisms vary. we studied the icu-acquired infections and their effect on patient outcomes in our icu.201626944004
first report of oxa-72-producing acinetobacter calcoaceticus in lebanon.emergence of carbapenem-resistant acinetobacter spp. has been increasingly reported worldwide. we report here the first detection of an acinetobacter calcoaceticus isolate from vegetables in lebanon carrying the bla oxa-72 gene. these findings show that the lebanese environment may constitute a potential reservoir for this antibiotic resistance gene.201626858838
endemicity of acinetobacter calcoaceticus-baumannii complex in an intensive care unit in malaysia.introduction. acinetobacter calcoaceticus-baumannii complex (acb complex) is a leading opportunistic pathogen in intensive care units (icus). effective control of spread requires understanding of its epidemiological relatedness. this study aims to determine the genetic relatedness and antibiotic susceptibilities of acb complex in an icu in malaysia. methodology. pulsed field gel electrophoresis (pfge), e-test, and disk diffusion were used for isolates characterization. results. during the study ...201526819759
characterization of carbapenem-resistant acinetobacter calcoaceticus-baumannii complex isolates from nosocomial bloodstream infections in southern iran.acinetobacter baumannii is an important opportunistic bacterial pathogen responsible for serious infections in hospitalized patients. from a total of 78 consecutive non-repetitive acinetobacter spp. isolates from patients with blood infections, 61 were carbapenem resistant, which were positive for blaoxa-51-like (96.7%), blaoxa-23-like (77 %), blaoxa-58-like (8.1%) and blaoxa-40-like genes (32.8%) by multiplex pcr. the isolates were identified as a. baumannii (n = 59) and acinetobacter nosocomia ...201626747061
enrichment of acinetobacter spp. from food samples.relatively little is known about the role of foods in the chain of transmission of acinetobacters and the occurrence of different acinetobacter spp. in foods. currently, there is no standard procedure to recover acinetobacters from food in order to gain insight into the food-related ecology and epidemiology of acinetobacters. this study aimed to assess whether enrichment in dijkshoorn enrichment medium followed by plating in chromagar™ acinetobacter medium is a useful method for the isolation of ...201626742623
utility of whole-genome sequencing in characterizing acinetobacter epidemiology and analyzing hospital outbreaks.acinetobacter baumannii frequently causes nosocomial infections and outbreaks. whole-genome sequencing (wgs) is a promising technique for strain typing and outbreak investigations. we compared the performance of conventional methods with wgs for strain typing clinical acinetobacter isolates and analyzing a carbapenem-resistant a. baumannii (crab) outbreak. we performed two band-based typing techniques (pulsed-field gel electrophoresis and repetitive extragenic palindromic-pcr), multilocus sequen ...201626699703
antimicrobial resistance profiles and oxacillinase genes in carbapenem-resistant acinetobacter baumannii isolated from hospitalized patients in santa catarina, brazil.carbapenems are the therapy of choice for treating severe infections caused by the acinetobacter calcoaceticus-acinetobacter baumannii complex. we aimed to assess the prevalence and antimicrobial susceptibility profiles of producers of distinct oxacillinases among nosocomial isolates of the a. calcoaceticus-a. baumannii complex in a 249-bed general hospital located in joinville, southern brazil.201526676494
biodegradation of ochratoxin a by bacterial strains isolated from vineyard soils.ochratoxin a (ota) is a mycotoxin with a main nephrotoxic activity contaminating several foodstuffs. in the present report, five soil samples collected from ota-contaminated vineyards were screened to isolate microorganisms able to biodegrade ota. when cultivated in ota-supplemented medium, ota was converted in otα by 225 bacterial isolates. to reveal clonal relationships between isolates, molecular typing by using an automated rep-pcr system was carried out, thus showing the presence of 27 diff ...201526633497
biofilm-associated proteins: news from acinetobacter.a giant protein called bap (biofilm-associated protein) plays a role in biofilm formation and adhesion to host cells in a. baumannii. most of the protein is made by arrays of 80-110 aa modules featuring immunoglobulin-like (ig-like) motifs.201526572057
effects of temperature and ph on the activities of catechol 2,3-dioxygenase obtained from crude oil contaminated soil in ilaje, ondo state, nigeria.enrichment technique was employed for the isolation of the crude oil degrading bacteria. the isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. specific activities of catechol-2,3-dioxygenase and effects of temperature and ph and their stabilities on the enzyme relative activities were observed. bacteria isolated from the soil sample include; bacillus cereus, b. amyloliquficiens, b. firmus, acinetobacter calcoaceticu ...201526464607
efficacy of lactoferricin b in controlling ready-to-eat vegetable spoilage caused by pseudomonas spp.the microbial content of plant tissues has been reported to cause the spoilage of ca. 30% of chlorine-disinfected fresh vegetables during cold storage. the aim of this work was to evaluate the efficacy of antimicrobial peptides in controlling microbial vegetable spoilage under cold storage conditions. a total of 48 bacterial isolates were collected from ready-to-eat (rte) vegetables and identified as belonging to acinetobacter calcoaceticus, aeromonas media, pseudomonas cichorii, pseudomonas flu ...201526453993
exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium.fungi and bacteria form various associations that are central to numerous environmental processes. in the so-called fungal highway, bacteria disperse along fungal mycelium. we developed a novel tool for the in situ isolation of bacteria moving along fungal hyphae as well as for the recovery of fungi potentially involved in dispersal, both of which are attracted towards a target culture medium. we present the validation and the results of the first in situ test. couples of fungi and bacteria were ...201526432804
species identification within acinetobacter calcoaceticus-baumannii complex using maldi-tof ms.acinetobacter baumannii, one of the more clinically relevant species in the acinetobacter genus is well known to be multi-drug resistant and associated with bacteremia, urinary tract infection, pneumonia, wound infection and meningitis. however, it cannot be differentiated from closely related species such as acinetobacter calcoaceticus, acinetobacter pittii and acinetobacter nosocomialis by most phenotypic tests and can only be differentiated by specific, time consuming genotypic tests with ver ...201526381662
bacteria present in comadia redtenbacheri larvae (lepidoptera: cossidae).the external and internal culturable bacterial community present in the larvae of comadia redtenbacheri hammerschmidt, an edible insect, was studied. characterization of the isolates determined the existence of 18 morphotypes and phylogenetic analysis of the 16s rrna gene revealed the existence of paenibacillus sp., bacillus safensis, pseudomonas sp., bacillus pseudomycoides, corynebacterium variabile, enterococcus sp., gordonia sp., acinetobacter calcoaceticus, arthrobacter sp., micrococcus sp. ...201526336239
synthesis and antibacterial evaluation of new thione substituted 1,2,4-triazole schiff bases as novel antimicrobial agents.the condensation reaction of 5-(4-aminophenyl)-4-phenyl-1,2,4-triazole-3-thione with salicylaldehyde, 4-hydroxybenzaldehyde, 5-chlorosalicylaldehyde, 5-bromosalicylaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde and 4-methoxybenzaldehyde in methanol results in series of new schiff bases. the structure of schiff bases were confirmed by (1)h nmr, (13)c nmr, ir and mass spectroscopy. the synthesized compounds were tested for their antimicrobial activity against bacterial (gr ...201526330857
phenotypic and molecular epidemiology of acinetobacter calcoaceticus baumannii complex strains spread at nemazee hospital of shiraz, iran.acinetobacter calcoaceticus baumannii (acb) complex are gram-negative opportunistic bacteria with low virulence properties. their resistance to antibiotics has become a matter of concern in hospital infections.201526322204
polypeptide requirement of multicomponent monooxygenase dsoabcdef for dimethyl sulfide oxidizing activity.in our previous study, the multicomponent monooxygenase dsoabcdef in acinetobacter sp. strain 20b was cloned based on its ability to oxidize dimethyl sulfide (dms) to dimethyl sulfoxide (dmso) in e. coli cells, which had high sequence similarity with phenol hydroxylase mopklmnop in acinetobacter calcoaceticus ncib8250, dmpklmnop in pseudomonas sp. cf600 and some other multicomponent monooxygenases. in this study, dsob, c, d, e, and f were found to be needed for dms-oxidizing activity in polypept ...199926300166
draft genome sequence of acinetobacter calcoaceticus strain gk1, a hydrocarbon-degrading plant growth-promoting rhizospheric bacterium.the 3.94-mb draft genome of acinetobacter calcoaceticus gk1, a hydrocarbonoclastic plant growth-promoting gram-negative rhizospheric bacterium, is presented here. isolated at the ford motor company site in genk, belgium, from poplar trees planted on a diesel-contaminated plume, gk1 is useful for enhancing hydrocarbon phytoremediation.201526272576
using vitek maldi-tof mass spectrometry to identify species belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex: a relevant alternative to molecular biology?acinetobacter baumannii belongs to the acinetobacter calcoaceticus-baumannii complex (acb) containing 2 other pathogenic species: acinetobacter pittii and acinetobacter nosocomialis. identification of these bacteria remains problematic despite the use of matrix-assisted laser ionization time-of-flight mass spectrometry (maldi-tof ms). here, we enriched the saramis™ database of the vitek ms® plus mass spectrometer to improve the identification of species of the acb complex. for each species, we i ...201526198329
an endophytic bacterium acinetobacter calcoaceticus sasm3-enhanced phytoremediation of nitrate-cadmium compound polluted soil by intercropping sedum alfredii with oilseed rape.intensive agricultural system with high input of fertilizer results in high agricultural output. however, excessive fertilization in intensive agricultural system has great potential to cause nitrate and heavy metal accumulation in soil, which is adverse to human health. the main objective of the present study was to observe the effects of intercropping and inoculation of endophytic bacterium acinetobacter calcoaceticus sasm3 on phytoremediation of combined contaminated soil in oilseed rape (bra ...201526146371
coaggregation between rhodococcus and acinetobacter strains isolated from the food industry.in this study, coaggregation interactions between rhodococcus and acinetobacter strains isolated from food-processing surfaces were characterized. rhodococcus sp. strain mf3727 formed intrageneric coaggregates with rhodococcus sp. strain mf3803 and intergeneric coaggregates with 2 strains of acinetobacter calcoaceticus (mf3293, mf3627). stronger coaggregation between a. calcoaceticus mf3727 and rhodococcus sp. mf3293 was observed after growth in batch culture at 30 °c than at 20 °c, after growth ...201526103135
[destruction of oil in the presence of cu2+ and surfactants of acinetobacter calcoaceticus imv b-7241, rhodococcus erythropolis imv ac-5017 and nocardia vaccinii imv b-7405].the effect of copper cations (0.01-1.0 mm) and surface-active agents (surfactants) of acinetobacter calcoaceticus imv b-7241, rhodococcus erythropolis imv alc-5017 and nocardia vaccinii imv b-7405 in the form of culture liquid on the destruction of oil in water (3.0-6.0 g/l) and soil (20 g/kg), including in the presence of cd2+ and pb2+ (0.01-0.5 mm), was investigated. it was shown that the degree of oil degradation in water and soil after 20 days in the presence of low concentrations of cu2+ (0 ...201526036026
long-term effectiveness of infection and antibiotic control programs on the transmission of carbapenem-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex in central taiwan.a carbapenem-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex (cra complex) infection is one of most the difficult infections to control worldwide. we evaluated the long-term effects of infection control interventions on the incidence densities of healthcare-associated infection (hai) and cra complex infection, and the rates of acinetobacter calcoaceticus-acinetobacter baumannii complex bacteremia (ab).201526028525
[antiadhesive properties of the surfactants of acinetobacter calcoaceticus imb b-7241, rhodococcus erythropolis imb ac-5017, and nocardia vaccinii imb b-7405].attachment of the cells of some bacteria, yeasts, and micromycetes to various surfaces (catheters, dentures, plastic, polyvinyl chloride, tiles, and steel) treated with the surfactants fromacinetobacter calcoace- ticus imb b-7241, rhodococcus erythropolis imb ac-5017, and nocardia vaccinii imb b-7405 was studied. adhesion of microorganisms to all the studied surfaces depended on the surfactant concentration and purity, kind of surface, and the test culture. treatment with the surfactants from n. ...201525941712
bacterial community structure in treated sewage sludge with mesophilic and thermophilic anaerobic digestion.stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. the aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (mad) and thermophilic anaerobic digestion (tad), on bacterial communities in sludge, including the presence of pathogenic microorganisms. bacterial community structure and phylogenetic diversity were analyzed in four sewage sludge samples from the czech republic. a ...201525921720
acinetobacter calcoaceticus from a fatal case of pneumonia harboring bla(ndm-1) on a widely distributed plasmid.we have recovered one bla(ndm-1)-harboring bacterial strain, designated as xm1570, from a sputum sample obtained from a fatal case of pneumonia in china.201525881070
molecular identification of phosphate-solubilizing native bacteria isolated from the rhizosphere of prosopis glandulosa in mexicali valley.one of the main limitations in intensive crop production in northwestern mexico is the dependence on the use of phosphate fertilizer. in this study, we isolated indigenous microorganisms with phosphate solubilization capacities from mesquite (prosopis glandulosa) present in the mexicali valley. in total, 4 bacteria were isolated from the rhizosphere of mesquite, including ica01, ica02ba, ica03bs, and ica04ma. the bacterial isolates were identified based on their phenotypic and 16s rrna gene sequ ...201525867428
isolation and identification of acinetobacter species with special reference to antibiotic resistance.acinetobacter is clinically important pathogen with widespread resistance to various antibiotics. we assessed the incidence of acinetobacter infection at a tertiary care hospital, analyze their resistance pattern and identify the production of extended spectrum β-lactamases (esbls) and metallo β-lactamases (mbls).201725810655
[overexpression, purification and characterization of phospholipase c from acinetobacter calcoaceticus].in this study, we constructed two recombinant escherichia coli strains to produce phospholipase c (plc) from acinetobacter calcoaceticus. the recombinant enzymes were purified to homogeneity and characterized. [methods] we cloned the plc encoding gene plc1, plc2 from genome dna of a. calcoaceticus atcc17902. the amplified fragments were inserted into pet28a(+ to obtain expression plasmids. e. coli bl21 (de3) harboring the above plasmids were cultivated and induced with isopropyl-beta-d-thiogalac ...201425803900
development of an observation platform for bacterial activity using polypyrrole films doped with bacteria.in our study, various bacteria, including gram-negative (pseudomonas aeruginosa, escherichia coli, acinetobacter calcoaceticus, serratia marcescens, shewanella oneidensis) and gram-positive (bacillus subtilis) bacteria, were straightforwardly immobilized into the conducting polymers (cps) by electrochemical deposition. the doping state of bacteria in the polymer films (polypyrrole and poly(3,4-ethylenedioxythiophene)) varied according to the polymerization conditions. the viability of bacteria i ...201525760406
sulbactam treatment for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex.multidrug-resistant (mdr) acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex has become an important cause of nosocomial pneumonia. sulbactam is a β-lactamase inhibitor with antimicrobial activity against mdr acb complex.201525746600
comparison between bacteremia caused by acinetobacter pittii and acinetobacter nosocomialis.patients with acinetobacter pittii and acinetobacter nosocomialis bacteremia have lower mortality rates than those with acinetobacter baumannii bacteremia. however, it is unknown whether these organisms differ in outcomes of bacteremic patients. we conducted this study to answer this question.201725735795
draft genome sequence of acinetobacter calcoaceticus strain p23, a plant growth-promoting bacterium of duckweed.acinetobacter calcoaceticus strain p23 is a plant growth-promoting bacterium, which was isolated from the surface of duckweed. we report here the draft genome sequence of strain p23. the genome data will serve as a valuable reference for understanding the molecular mechanism of plant growth promotion in aquatic plants.201525720680
acinetobacter seifertii sp. nov., a member of the acinetobacter calcoaceticus-acinetobacter baumannii complex isolated from human clinical specimens.this study aimed to define the taxonomic status of a phenetically distinct group of 16 strains that corresponds to acinetobacter genomic species 'close to 13tu', a provisional genomic species of the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex recognized by gerner-smidt and tjernberg in 1993. these strains have been isolated in different countries since the early 1990s and were mostly recovered from human clinical specimens. they were compared with 45 reference strains repre ...201525563912
antibiotic susceptibility and molecular epidemiology of acinetobacter calcoaceticus-baumannii complex strains isolated from a referral hospital in northern vietnam.acinetobacter calcoaceticus-baumannii complex is a common cause of hospital-acquired infections (hais) globally, remarkable for its high rate of antibiotic resistance, including to carbapenems. there are few data on the resistance of a. baumannii in vietnam, which are essential for developing evidence-based treatment guidelines for hais. antibiotic susceptibility testing was conducted by vitek(®)2, and pulsed-field gel electrophoresis (pfge) was performed on 66 clinical a. baumannii complex isol ...201425540720
a new double digestion ligation mediated suppression pcr method for simultaneous bacteria dna-typing and confirmation of species: an acinetobacter sp. model.we have designed a new ddlms pcr (double digestion ligation mediated suppression pcr) method based on restriction site polymorphism upstream from the specific target sequence for the simultaneous identification and differentiation of bacterial strains. the ddlms pcr combines a simple pcr used for species or genus identification and the lm pcr strategy for strain differentiation. the bacterial identification is confirmed in the form of the pcr product(s), while the length of the pcr product makes ...201425522278
substitutions of ser83leu in gyra and ser80leu in parc associated with quinolone resistance in acinetobacter pittii.to investigate the prevalence and the mechanism of quinolone-resistant acinetobacter pittii, 634 acinetobacter calcoaceticus-acinetobacter baumannii complex isolates were collected throughout zhejiang province. identification of isolates was conducted by matrix-assisted laser desorption ionization/time of flight mass spectrometry (maldi-tof ms), blaoxa-51-like gene, and partial rna polymerase β-subunit (rpob) amplification. twenty-seven isolates of a. pittii were identified. among the 634 isolat ...201525514581
epidemiological characteristics of blandm-1 in enterobacteriaceae and the acinetobacter calcoaceticus-acinetobacter baumannii complex in china from 2011 to 2012.the study aimed to investigate the prevalence and epidemiological characteristics of blandm-1 (encoding new delhi metallo-β-lactamase 1) in enterobacteriaceae and the acinetobacter calcoaceticus-acinetobacter baumannii complex (abc) in china from july 2011 to june 2012.201425469701
structural and physiochemical characterization of rhamnolipids produced by acinetobacter calcoaceticus, enterobacter asburiae and pseudomonas aeruginosa in single strain and mixed cultures.rhamnolipids are naturally occurring biosurfactants with a wide range of potential commercial applications. as naturally derived products they present an ecological alternative to synthetic surfactants. the majority of described rhamnolipid productions are single strain pseudomonas spp. cultivations. here we report rhamnolipids producing bacteria acinetobacter calcoaceticus, enterobacter asburiae and pseudomonas aeruginosa that were cultivated separately and as mixed populations. the ratio and c ...201525433178
detection of ndm-1 carbapenemase-producing acinetobacter calcoaceticus and acinetobacter junii in environmental samples from livestock farms. 201525349061
high prevalence of new delhi metallo-β-lactamase in acinetobacter calcoaceticus-a. baumannii complex at two tertiary care centres in north india. 201425297039
evaluation of clonality and carbapenem resistance mechanisms among acinetobacter baumannii-acinetobacter calcoaceticus complex and enterobacteriaceae isolates collected in european and mediterranean countries and detection of two novel β-lactamases, ges-22 and vim-35.we evaluated doripenem-resistant acinetobacter baumannii-acinetobacter calcoaceticus complex (acb; n = 411) and enterobacteriaceae (n = 92) isolates collected from patients from 14 european and mediterranean countries during 2009 to 2011 for the presence of carbapenemase-encoding genes and clonality. following susceptibility testing, carbapenem-resistant (doripenem mic, >2 μg/ml) isolates were screened for carbapenemases. new β-lactamase genes were expressed in a common background and susceptibi ...201425267671
influence of acb complex genospecies on clinical outcomes in a u.s. hospital with high rates of multidrug resistance.bacteria within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex commonly cause nosocomial infection and are often multidrug resistant. advances in genospecies typing allow for speciation within the acb complex; however, little is known about the effect of genospecies on patient outcomes.201525246361
growth characteristics and biofilm formation of various spoilage bacteria isolated from fresh produce.this study investigated the characteristics of spoilage bacteria isolated from fresh produce including growth at various temperatures, biofilm formation, cell hydrophobicity, and colony spreading. the number of spoilage bacteria present when stored at 35 °c was significantly greater than when stored at lower temperatures, and maximum population size was achieved after 10 h. however, bacillus pumilus, dickeya zeae, pectobacterium carotovorum subsp. carotovorum pcc21, and bacillus pumilus (rda-r) ...201425224561
determination of cypermethrin degradation potential of soil bacteria along with plant growth-promoting characteristics.the pyrethroid insecticide cypermethrin is in extensive use since 1980s for insect control. however, its toxicity toward aquatic animals and humans requires its complete removal from contaminated areas that can be done using indigenous microbes through bioremediation. in this study, three bacterial strains isolated from agricultural soil and identified as acinetobacter calcoaceticus mcm5, brevibacillus parabrevis fcm9, and sphingomonas sp. rcm6 were found highly efficient in degrading cypermethr ...201525194282
characterization and plasmid elimination of ndm-1-producing acinetobacter calcoaceticus from china.the presence of multidrug-resistant bacterial pathogens in the environment poses a serious threat to public health. the opportunistic acinetobacter spp. are among the most prevalent causes of nosocomial infections. here, we performed complete genome sequencing of the acinetobacter calcoaceticus strain xm1570, which was originally cultivated from the sputum of a patient diagnosed with pneumonia in xiamen in 2010. we identified carbapenem resistance associated gene bla(ndm-1) located on a 47.3-kb ...201425181293
insertions or deletions (indels) in the rrn 16s-23s rrna gene internal transcribed spacer region (its) compromise the typing and identification of strains within the acinetobacter calcoaceticus-baumannii (acb) complex and closely related members.to determine whether its sequences in the rrn operon are suitable for identifying individual acinetobacter acb complex members, we analysed length and sequence differences between multiple its copies within the genomes of individual strains. length differences in its reported previously between a. nosocomialis bcrc15417t (615 bp) and other strains (607 bp) can be explained by presence of an insertion (indel 13i/1) in the longer its variant. the same indel 13i/1 was also found in its sequences of ...201425141005
bacterial community analysis in chlorpyrifos enrichment cultures via dgge and use of bacterial consortium for cp biodegradation.the organophosphate pesticide chlorpyrifos (cp) has been used extensively since the 1960s for insect control. however, its toxic effects on mammals and persistence in environment necessitate its removal from contaminated sites, biodegradation studies of cp-degrading microbes are therefore of immense importance. samples from a pakistani agricultural soil with an extensive history of cp application were used to prepare enrichment cultures using cp as sole carbon source for bacterial community anal ...201425008559
[synthesis of surfactants by rhodococcus erythropolis imv ac-5017, acinetobacter calcoaceticus imv b-7241 and nocardia vaccinii imv b-7405 on industrial waste].the synthesis of surfactants by rhodococcus erythropolis imv ac-5017, acinetobacter calcoaceticus imv b-7241 and nocardia vaccinii imv b-7405 on industrial waste (food and oil-processing industry, production of biodiesel) was investigated. the possibility of replacing the expensive substrates (n-hexadecane and ethanol) by industrial waste (oil and fat industry, fried sunflower oil, glycerol, liquid paraffin) for the surfactant biosynthesis was established. the conditional concentration of surfac ...201625000725
rapid identification of acinetobacter baumannii, acinetobacter nosocomialis and acinetobacter pittii with a multiplex pcr assay.acinetobacter baumannii, acinetobacter nosocomialis and acinetobacter pittii are clinically relevant members of the acinetobacter calcoaceticus-a. baumannii (acb) complex and important nosocomial pathogens. these three species are genetically closely related and phenotypically similar; however, they differ in their epidemiology, antibiotic resistance and pathogenicity. in this study, we investigated the use of a multiplex pcr-based assay designed to detect internal fragments of the 16s-23s rrna ...201424965800
characterization of affinity-purified isoforms of acinetobacter calcoaceticus y1 glutathione transferases.glutathione transferases (gst) were purified from locally isolated bacteria, acinetobacter calcoaceticus y1, by glutathione-affinity chromatography and anion exchange, and their substrate specificities were investigated. sds-polyacrylamide gel electrophoresis revealed that the purified gst resolved into a single band with a molecular weight (mw) of 23 kda. 2-dimensional (2-d) gel electrophoresis showed the presence of two isoforms, gst1 (pi 4.5) and gst2 (pi 6.2) with identical mw. gst1 was reac ...201424892084
characterization and fungal inhibition activity of siderophore from wheat rhizosphere associated acinetobacter calcoaceticus strain hirfa32.acinetobacter calcoaceticus hirfa32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without feso4 at 28 °c and 24 h of incubation. hplc purified siderophore appeared as pale yellow crystals with molecular weight [m(+1)] m/z 347.18 estimated by lcms. the structure elucidated by (1)h nmr, (13)c nmr, hmqc, hmbc, noesy and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid w ...201424891739
risk factors and outcomes for patients with bloodstream infection due to acinetobacter baumannii-calcoaceticus complex.identifying patients at risk for bloodstream infection (bsi) due to acinetobacter baumannii-acinetobacter calcoaceticus complex (abc) and providing early appropriate therapy are critical for improving patient outcomes. a retrospective matched case-control study was conducted to investigate the risk factors for bsi due to abc in patients admitted to the detroit medical center (dmc) between january 2006 and april 2009. the cases were patients with bsi due to abc; the controls were patients not inf ...201424890594
maldi-tof ms and chemometric based identification of the acinetobacter calcoaceticus-acinetobacter baumannii complex species.maldi-tof ms is becoming the technique of choice for rapid bacterial identification at species level in routine diagnostics. however, some drawbacks concerning the identification of closely related species such as those belonging to the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex lead to high rates of misidentifications. in this work we successfully developed an approach that combines maldi-tof ms and chemometric tools to discriminate the six acb complex species (a. baumann ...201424877727
class 1 integrons and antibiotic resistance of clinical acinetobacter calcoaceticus-baumannii complex in poznań, poland.sixty-three clinical isolates of acinetobacter calcoaceticus-baumannii complex were analyzed for the presence of integrons and antimicrobial resistance. class 1 integrons were detected in 40 (63.5 %) isolates. none of them had class 2 or class 3 integrons. the majority of the integrons contained aacc1-orfa-orfb-aada1 gene cassette array. the presence of integrons was associated with the increased frequency of resistance to 12 of 15 antimicrobials tested, multi-drug resistance phenotype, and the ...201424740302
occurrence of plasmids in the aromatic degrading bacterioplankton of the baltic sea.plasmids are mobile genetic elements that provide their hosts with many beneficial traits including in some cases the ability to degrade different aromatic compounds. to fulfill the knowledge gap regarding catabolic plasmids of the baltic sea water, a total of 209 biodegrading bacterial strains were isolated and screened for the presence of these mobile genetic elements. we found that both large and small plasmids are common in the cultivable baltic sea bacterioplankton and are particularly prev ...201124710296
heterologous expression of pyrroloquinoline quinone (pqq) gene cluster confers mineral phosphate solubilization ability to herbaspirillum seropedicae z67.gluconic acid secretion mediated by the direct oxidation of glucose by pyrroloquinoline quinone (pqq)-dependent glucose dehydrogenase (gdh) is responsible for mineral phosphate solubilization in gram-negative bacteria. herbaspirillum seropedicae z67 (atcc 35892) genome encodes gdh apoprotein but lacks genes for the biosynthesis of its cofactor pqq. in this study, pqqe of erwinia herbicola (in plasmid pjnk1) and pqq gene clusters of pseudomonas fluorescens b16 (pok53) and acinetobacter calcoaceti ...201424682480
surface water isolates of hemolytic and non-hemolytic acinetobacter with multiple drug and heavy metal resistance ability.acinetobacter in surface waters are a major concern because of their rapid development of resistance to a wide range of antimicrobials and their ability to persist in these waters for a very long time. four surface water isolates of acinetobacter having both multidrug- and multimetal-resistant ability were isolated and identified through biochemical tests and 16s rdna sequencing. based on these analyses, two hemolytic isolates were affiliated with acinetobacter haemolyticus with an accession num ...201424642427
air-lift reactor system for the treatment of waste-gas-containing monochlorobenzene.an air-lift bioreactor (alr) system, applied for the treatment of waste-gas-containing monochlorobenzene (mcb) was seeded with pure culture of acinetobacter calcoaceticus, isolated from soil as a starter seed. it was found that mcb was biologically converted to chloride as chloride was mineralized in the alr. after the built up of the biomass in the alr, the reactor parameters which have major influence on the removal efficiency and elimination capacity were studied using response surface method ...201424617061
discrimination of the acinetobacter calcoaceticus-acinetobacter baumannii complex species by fourier transform infrared spectroscopy.the main goal of this work was to assess the ability of fourier transform infrared spectroscopy with attenuated total reflectance (ftir-atr) to discriminate between the species of the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex, i.e. a. baumannii, a. nosocomialis, a. pittii, a. calcoaceticus, genomic species "between 1 and 3" and genomic species "close to 13tu". a total of 122 clinical isolates of the acb complex previously identified by rpob sequencing were studied. ftir-a ...201424563251
improved homology model of cyclohexanone monooxygenase from acinetobacter calcoaceticus based on multiple templates.a new homology model of cyclohexanone monooxygenase (chmo) from acinetobacter calcoaceticus is derived based on multiple templates, and in particular the crystal structure of chmo from rhodococcus sp. the derived model was fully evaluated, showing that the quality of the new structure was improved over previous models. critically, the nicotinamide cofactor is included in the model for the first time. analysis of several molecular dynamics snapshots of intermediates in the enzymatic mechanism led ...201424530814
rapid identification of acinetobacter spp. by fluorescence in situ hybridization (fish) from colony and blood culture material.multi-drug-resistant strains of the acinetobacter baumannii complex cause nosocomial infections. rapid identification of acinetobacter spp. is desirable in order to facilitate therapeutic or hygiene decisions. we evaluated a newly designed dna probe that can be used under standard conditions in both a microwave oven and a slide chamber for the rapid identification of acinetobacter spp. by fluorescence in situ hybridization (fish). using fish, the new probe correctly identified 81/81 acinetobacte ...201124516735
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