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diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in liangshui river of beijing, china.aerobic denitrification is a process reducing the nitrate into gaseous nitrogen forms in the presence of oxygen gas, which makes the nitrification and denitrification performed simultaneously. however, little was known on the diversity of the culturable aerobic denitrifying bacteria in the surface water system. in this study, 116 strains of aerobic denitrifying bacteria were isolated from the sediment, water and biofilm samples in liangshui river of beijing. these bacteria were classified into 1 ...201728855587
effects of humic acid on the interactions between zinc oxide nanoparticles and bacterial biofilms.the effects of humic acid (ha) on interactions between zno nanoparticles (zno nps) and pseudomonas putida kt2440 biofilms at different maturity stages were investigated. three stages of biofilm development were identified according to bacterial adenosine triphosphate (atp) activity associated with biofilm development process. in the initial biofilm stage 1, the atp content of bacteria was reduced by more than 90% when biofilms were exposed to zno nps. however, in the mature biofilm stages 2 and ...201728851497
growth phase-dependent expression profiles of three vital h-ns family proteins encoded on the chromosome of pseudomonas putida kt2440 and on the pcar1 plasmid.h-ns family proteins are nucleoid-associated proteins that form oligomers on dna and function as global regulators. they are found in both bacterial chromosomes and plasmids, and were suggested to be candidate effectors of the interaction between them. tura and turb are the predominantly expressed h-ns family proteins encoded on the chromosome of pseudomonas putida kt2440, while pmr is encoded on the carbazole-degradative incompatibility group p-7 plasmid pcar1. previous transcriptome analyses s ...201728851281
a bacterial multi-domain nad-independent d-lactate dehydrogenase utilizes fad and fe-s clusters as cofactors and quinone as electron acceptor for d-lactate oxidization.bacterial membrane-associated nad-independent d-lactate dehydrogenase (fe-s d-ildh) oxidizes d-lactate into pyruvate. sequence analysis of the enzyme reveals that it contains a fe-s oxidoreductase domain in addition to an fad-containing dehydrogenase domain, which differs from other typical d-ildhs. fe-s d-ildh from pseudomonas putida kt2440 was purified as a his-tagged protein and characterized in detail. this monomeric enzyme exhibited activities with l-lactate and several d-2-hydroxyacids. qu ...201728847921
electrochemical hydroxylation of c3-c12 n-alkanes by recombinant alkane hydroxylase (alkb) and rubredoxin-2 (alkg) from pseudomonas putida gpo1.an unprecedented method for the efficient conversion of c3-c12 linear alkanes to their corresponding primary alcohols mediated by the membrane-bound alkane hydroxylase (alkb) from pseudomonas putida gpo1 is demonstrated. the x-ray absorption spectroscopy (xas) studies support that electrons can be transferred from the reduced alkg (rubredoxin-2, the redox partner of alkb) to alkb in a two-phase manner. based on this observation, an approach for the electrocatalytic conversion from alkanes to alc ...201728827709
heterologous expression of phac(2) gene and poly-3-hydroxyalkanoate production by recombinant cupriavidus necator strains using canola oil as carbon source.many heterologous transformation studies have been carried out using the cupriavidus necator phb(-4) strain to investigate the expression characteristics of various polyhydroxyalkanoate (pha) synthase enzymes. in this study, we generated a recombinant c. necator phb(-4) strain by transforming a plasmid (pmrc03) harbouring the synthetic phac2 gene of pseudomonas putida ca-3. under conditions favourable for expression of the phac2 p.putca-3 gene, canola oil was used as carbon source for the synthe ...201728827158
crystal structures reveal metal-binding plasticity at the metallo-β-lactamase active site of pqqb from pseudomonas putida.pqqb is an enzyme involved in the biosynthesis of pyrroloquinoline quinone and a distal member of the metallo-β-lactamase (mbl) superfamily. pqqb lacks two residues in the conserved signature motif hxhxdh that makes up the key metal-chelating elements that can bind up to two metal ions at the active site of mbls and other members of its superfamily. here, we report crystal structures of pqqb bound to mn(2+), mg(2+), cu(2+), and zn(2+). these structures demonstrate that pqqb can still bind metal ...201728825148
oil biodegradation: interactions of artificial marine snow, clay particles, oil and corexit.during the deepwater horizon (dwh) oil spill, interactions between oil, clay particles and marine snow lead to the formation of aggregates. interactions between these components play an important, but yet not well understood, role in biodegradation of oil in the ocean water. the aim of this study is to explore the effect of these interactions on biodegradation of oil in the water. laboratory experiments were performed, analyzing respiration and n-alkane and btex biodegradation in multiple condit ...201728821355
pseudomonas wadenswilerensis sp. nov. and pseudomonas reidholzensis sp. nov., two novel species within the pseudomonas putida group isolated from forest soil.within the frame of a biotechnological screening, we isolated two pseudomonas strains from forest soil. 16s rrna gene sequence analysis indicated that strain ccos 864t shared 99.8 % similarity with pseudomonas donghuensis hyst, while strain ccos 865t shared 99.0 % similarity with pseudomonas putida dsm 291t and lower similarity with other p. putida group type strains. based on multilocus sequence analysis, the two strains were genotypically distinct from each other, each forming a separate clade ...201728820109
in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis niloticus.fish gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ...201728819794
photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of synechococcus elongatus cscb and pseudomonas putida cscab.one of the major challenges for the present and future generations is to find suitable substitutes for the fossil resources we rely on today. cyanobacterial carbohydrates have been discussed as an emerging renewable feedstock in industrial biotechnology for the production of fuels and chemicals, showing promising production rates when compared to crop-based feedstock. however, intrinsic capacities of cyanobacteria to produce biotechnological compounds are limited and yields are low.201728814973
oxygen mass transfer and shear stress effects on pseudomonas putida bcrc 14365 growth to improve bioreactor design and performance.in this work, the experimental evidence is presented for two basic issues including oxygen mass transfer and shear analysis on the microorganism containing medium on the most prominent sections of the bioreactor. computational fluid dynamics (cfd) methodology reproduces shear rate values for specific impeller designs using the commercial code (fluent 6.2). cfd calculates volumetric mass transfer coefficient based on the higbie's penetration theory. four types of impeller are used. the spherical ...201728803423
identification and elucidation of in vivo function of two alanine racemases from pseudomonas putida kt2440.the genome of pseudomonas putida kt2440 contains two open reading frames (orfs), pp_3722 and pp_5269, that encode proteins with a pyridoxal phosphate (plp) binding motif and a high similarity to alanine racemases. alanine racemases play a key role in the biosynthesis of d-alanine, a crucial amino acid in the peptidoglycan layer. for these orfs, we generated single and double mutants and found that inactivation of pp_5269 resulted in d-alanine auxotrophy, while inactivation of pp_3722 did not. fu ...201728799718
inactivation combined with cell lysis of pseudomonas putida using a low pressure carbon dioxide microbubble technology.inactivation processes can be classified into non-thermal inactivation methods such as ethylene oxide and γ-radiation, and thermal methods such as autoclaving. the ability of carbon dioxide enriched microbubbles to inactivate pseudomonas putida suspended in physiological saline, as a non-thermal sterilisation method, was investigated in this study with many operational advantages over both traditional thermal and non-thermal sterilisation methods.201728781404
production, biophysical characterization and crystallization of pseudomonas putida graa and its complexes with grat and the grata operator.the grata operon from pseudomonas putida encodes a toxin-antitoxin module with an unusually moderate toxin. here, the production, saxs analysis and crystallization of the antitoxin graa, the grata complex and the complex of graa with a 33 bp operator fragment are reported. graa forms a homodimer in solution and crystallizes in space group p21, with unit-cell parameters a = 66.9, b = 48.9, c = 62.7 å, β = 92.6°. the crystals are likely to contain two graa dimers in the asymmetric unit and diffrac ...201728777088
the repetitive detection of toluene with bioluminescence bioreporter pseudomonas putida tva8 encapsulated in silica hydrogel on an optical fiber.living cells of the lux-based bioluminescent bioreporter pseudomonas putida tva8 were encapsulated in a silica hydrogel attached to the distal wider end of a tapered quartz fiber. bioluminescence of immobilized cells was induced with toluene at high (26.5 mg/l) and low (5.3 mg/l) concentrations. initial bioluminescence maxima were achieved after >12 h. one week after immobilization, a biofilm-like layer of cells had formed on the surface of the silica gel. this resulted in shorter response times ...201628773598
chemoenzymatic synthesis of a novel borneol-based polyester.terpenes are a class of natural compounds that have recently moved into the focus as a bio-based resource for chemical production, owing to their abundance, their mostly cyclic structures, and the presence of olefin or single hydroxy groups. to apply this raw material in new industrial fields, a second hydroxy group is inserted into borneol by cytochrome p450cam (cyp101) enzymes in a whole-cell catalytic biotransformation with pseudomonas putida kt2440. next, a semi-continuous batch system was d ...201728772002
mass cytometry for detection of silver at the bacterial single cell level.background: mass cytometry (cytometry by time of flight, cytof) allows single-cell characterization on the basis of specific metal-based cell markers. in addition, other metals in the mass range such as silver can be detected per cell. bacteria are known to be sensible to silver and a protocol was developed to measure both the number of affected cells per population and the quantities of silver per cell. methods: for mass cytometry ruthenium red was used as a marker for all cells of a population ...201728769897
a novel strategy to immobilize bacteria on polymer particles for efficient adsorption and biodegradation of soluble organics.a novel strategy was used to immobilize bacterial cells on the surface of functional polymer particles for the efficient adsorption and biodegradation of organics in wastewater. first, the bacterial cells were aggregated using a vinyl-containing pre-polymer, and the obtained bacteria-pre-polymer complex was then used as a particle stabilizer to construct a stable pickering emulsion of functional cross-linking monomers and hydrophobic superparamagnetic iron oxide nanoparticles (the oil phase) in ...201728767111
evaluation of bioaugmentation and biostimulation on arsenic remediation in soil through biovolatilization.arsenic (as) removal through microbially driven biovolatilization can be explored as a potential method for as bioremediation. however, its effectiveness needs to be improved. biostimulation with organic matter amendment and bioaugmentation with the inoculation of genetic engineered bacteria could be potential strategies for as removal and site remediation. here, the experiments were conducted to evaluate the impacts of rice straw and biochar amendment, inoculation of genetic engineered pseudomo ...201728766144
combinatorial metabolic engineering of pseudomonas putida kt2440 for efficient mineralization of 1,2,3-trichloropropane.an industrial waste, 1,2,3-trichloropropane (tcp), is toxic and extremely recalcitrant to biodegradation. to date, no natural tcp degraders able to mineralize tcp aerobically have been isolated. in this work, we engineered a biosafety pseudomonas putida strain kt2440 for aerobic mineralization of tcp by implantation of a synthetic biodegradation pathway into the chromosome and further improved tcp mineralization using combinatorial engineering strategies. initially, a synthetic pathway composed ...201728765600
a versatile one-step crispr-cas9 based approach to plasmid-curing.plasmids are widely used and essential tools in molecular biology. however, plasmids often impose a metabolic burden and are only temporarily useful for genetic engineering, bio-sensing and characterization purposes. while numerous techniques for genetic manipulation exist, a universal tool enabling rapid removal of plasmids from bacterial cells is lacking.201728764701
molecular investigation on the binding of cd(ii) by the binary mixtures of montmorillonite with two bacterial species.bacteria-phyllosilicate complexes are commonly found in natural environments and are capable of immobilizing trace metals. however, the molecular binding mechanisms of heavy metals to these complex aggregates still remain poorly understood. this study investigated cd adsorption on gram-positive bacillus subtilis, gram-negative pseudomonas putida and their binary mixtures with montmorillonite using surface complexation model, cd k-edge extended x-ray absorption fine structure (exafs) spectroscopy ...201728754562
exacerbation of bronchiectasis by pseudomonas monteilii: a case report.pseudomonas spp are important opportunistic and nosocomial pathogens. one such species is pseudomonas monteilii (p. monteilii). it has been described as an environmental contaminant and potential pathogen. we identified this organism as the causative agent of an exacerbation of bronchiectasis and an environmental contaminant in our hospital on two separate occasions.201728738817
municipal wastewater treatment via co-immobilized microalgal-bacterial symbiosis: microorganism growth and nutrients removal.a symbiotic microalgal-bacterial system may be an optional technology for wastewater treatment. in this study, co-immobilized of a bacterium isolated from a municipal wastewater treatment plant (pseudomonas putida) and a microalgae chlorella vulgaris was used in the study of cell growth and nutrient removal during wastewater treatment under batch and continuous culture conditions. under batch culture conditions, co-immobilization treatment significantly increased the cell density of c. vulgaris ...201728738545
surface complexation modeling of cu(ii) sorption to montmorillonite-bacteria composites.surface complexation modeling, isothermal titration calorimetry, and batch adsorption were employed to characterize the adsorption of cu onto montmorillonite, pseudomonas putida x4, and their composites at mass ratios of 2:1, 6:1, and 12:1, respectively. different enthalpy values were found for cu adsorption to permanent (-6.43kj/mol) and variable charge sites (8.51kj/mol) on montmorillonite. the component additivity (ca) method was used to predict the adsorption of cu on the composites by combi ...201728738531
gene expression during btex biodegradation by a microbial consortium acclimatized to unleaded gasoline and a pseudomonas putida strain (hm346961) isolated from it.pseudomonas putida strain (hm346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. the consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (btex) isomers, and a similar capability was observed with the p. putida strain. proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon compounds. furthermore, the toluene di-oxygenase (tod) gene was up-regulated in p. putida strain wh ...201728735314
transcriptional modulation of transport and metabolism associated gene clusters leading to utilization of benzoate in preference to glucose in pseudomonas putida csv86.the effective elimination of xenobiotic pollutants from the environment can be achieved by efficient degradation by microorganisms even in the presence of sugars or organic acids. pseudomonas putida csv86 displays a unique property to utilize aromatic compounds prior to glucose. the draft genome and transcription analyses revealed that glucose uptake and benzoate transport and metabolism genes are clustered at glc and ben loci, respectively, as two distinct operons. when grown on glucose+benzoat ...201728733285
construction and optimization of a heterologous pathway for protocatechuate catabolism in escherichia coli enables bioconversion of model aromatic compounds.the production of biofuels from lignocellulose yields a substantial lignin byproduct stream that currently has few applications. biological conversion of lignin-derived compounds into chemicals and fuels has the potential to improve the economics of lignocellulose-derived biofuels, but few microbes are able both to catabolize lignin-derived aromatic compounds and generate valuable products. while escherichia coli has been engineered to produce a variety of fuels and chemicals, it is incapable of ...201728733280
foam adsorption as an ex situ capture step for surfactants produced by fermentation.in this report, a method for a simultaneous production and separation of a microbially synthesized rhamnolipid biosurfactant is presented. during the aerobic cultivation of flagella-free pseudomonas putida em383 in a 3.1l stirred tank reactor on glucose as a sole carbon source, rhamnolipids are produced and excreted into the fermentation liquid. here, a strategy for biosurfactant capture from rhamnolipid enriched fermentation foam using hydrophobic-hydrophobic interaction was investigated. five ...201728723386
biofilm development of an opportunistic model bacterium analysed at high spatiotemporal resolution in the framework of a precise flow cell.life of bacteria is governed by the physical dimensions of life in microscales, which is dominated by fast diffusion and flow at low reynolds numbers. microbial biofilms are structurally and functionally heterogeneous and their development is suggested to be interactively related to their microenvironments. in this study, we were guided by the challenging requirements of precise tools and engineered procedures to achieve reproducible experiments at high spatial and temporal resolutions. here, we ...201628721252
antimicrobial and antibiofilm efficacy of self-cleaning surfaces functionalized by tio2 photocatalytic nanoparticles against staphylococcus aureus and pseudomonas putida.a photocatalytic sol of tio2 nanoparticles has been used for creating self-cleaning antimicrobial flat and porous glass surfaces. the substrates were irradiated to study their photocatalytic properties and behavior in the presence of biofilm-forming bacteria. smooth glass surfaces and glass microfiber filters were covered with 1.98×10(-3)±1.5×10(-4)gcm(-2) and 8.55×10(-3)±3.0×10(-4)gcm(-2) densities, respectively. self-cleaning properties were analyzed using the methylene blue 365nm uv-a photode ...201728715739
evaluation of nutrients removal (no3-n, nh3-n and po4-p) with chlorella vulgaris, pseudomonas putida, bacillus cereus and a consortium of these microorganisms in the treatment of wastewater effluents.in this research removal of nh3-n, no3-n and po4-p nutrients from municipal wastewater was studied, using chlorella vulgaris, pseudomonas putida, bacillus cereus and an artificial consortium of them. the objective is to analyze the performance of these microorganisms and their consortium, which has not been previously studied for nutrient removal in municipal wastewater. a model wastewater was prepared simulating the physicochemical characteristics found at the wastewater plant in chapala, mexic ...201728708609
pseudomonas aestus sp. nov., a plant growth-promoting bacterium isolated from mangrove sediments.strain cmaa 1215(t), a gram-reaction-negative, aerobic, catalase positive, polarly flagellated, motile, rod-shaped (0.5-0.8 × 1.3-1.9 µm) bacterium, was isolated from mangrove sediments, cananéia island, brazil. analysis of the 16s rrna gene sequences showed that strain cmaa 1215(t) forms a distinct phyletic line within the pseudomonas putida subclade, being closely related to p. plecoglossicida atcc 700383(t), p. monteilii nbrc 103158(t), and p. taiwanensis bcrc 17751(t) of sequence similarity ...201728702688
dual infection by burkholderia cepaciaand pseudomonas putida in an infective endocarditis case.infective endocarditis is rarely caused by burkholderia cepacia. pseudomonas putidahas not been reported to cause infective endocarditis so far. this is the first case of infective endocarditis being reported, that is caused by pseudomonas putidaand burkholderia cepaciain an immunocompetent host with no predisposing factors. aortic valve replacement surgery was carried out and antibiotics were given, to which the patient responded well and recovered.201728689527
erratum to: enhancing the catalytic potential of nitrilase from pseudomonas putida for stereoselective nitrile hydrolysis. 201728688045
synthesis and enzymatic ketonization of the 5-(halo)-2-hydroxymuconates and 5-(halo)-2-hydroxy-2,4-pentadienoates.5-halo-2-hydroxymuconates and 5-halo-2-hydroxy-2,4-pentadienoates are stable dienols that are proposed intermediates in bacterial meta-fission pathways for the degradation of halogenated aromatic compounds. the presence of the halogen raises questions about how the bulk and/or electronegativity of these substrates would affect enzyme catalysis or whether some pathway enzymes have evolved to accommodate it. to address these questions, 5-halo-2-hydroxymuconates and 5-halo-2-hydroxy-2,4-pentadienoa ...201728684981
a paradigm for ch bond cleavage: structural and functional aspects of transition state stabilization by mandelate racemase.mandelate racemase (mr) from pseudomonas putida catalyzes the mg(2+)-dependent, 1,1-proton transfer reaction that racemizes (r)- and (s)-mandelate. mr shares a partial reaction (i.e., the metal ion-assisted, brønsted base-catalyzed proton abstraction of the α-proton of carboxylic acid substrates) and structural features ((β/α)7β-barrel and n-terminal α + β capping domains) with a vast group of homologous, yet functionally diverse, enzymes in the enolase superfamily. mechanistic and structural st ...201728683916
developing a genetically encoded, cross-species biosensor for detecting ammonium and regulating biosynthesis of cyanophycin.responding to nitrogen status is essential for all living organisms. bacteria have evolved various complex and exquisite regulatory systems to control nitrogen metabolism. however, natural nitrogen regulatory systems, owing to their complexity, often function only in their original hosts and do not respond properly when transferred to another species. by harnessing the lactococcus glnra system, we developed a genetically encoded, cross-species ammonium biosensor that displays a dynamic range up ...201728683543
the pseudomonas putida csra/rsma homologues negatively affect c-di-gmp pools and biofilm formation through the ggdef/eal response regulator cfcr.expression of cfcr, encoding the only ggdef/eal response regulator in pseudomonas putida, is transcriptionally regulated by rpos, anr and fleq, and the functionality of cfcr as a diguanylate cyclase requires the multisensor chase3/gaf hybrid histidine kinase named cfca. here an additional level of cfcr control, operating post-transcriptionally via the rna-binding proteins rsma, rsme and rsmi, is unraveled. specific binding of the three proteins to an rsm-binding motif (5'canggang3') encompassing ...201728677348
degradation and conversion of endosulfan by newly isolated pseudomonas mendocina zam1 strain.endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. therefore, the aim of this study was to isolate and identify a novel bacterial strain capable of degrading endosulfan in agriculture contaminated soils. a novel bacterial strain was isolated from the sugarcane field contaminated with endosulfan, and was named as zam1 strain. the zam1 bacterial strain was further identified as pseudomonas men ...201728667651
cleaning and disinfection of biofilms composed of listeria monocytogenes and background microbiota from meat processing surfaces.surfaces of food processing premises are exposed to regular cleaning and disinfection (c&d) regimes, using biocides that are highly effective against bacteria growing as planktonic cells. however, bacteria growing in surface associated communities (biofilms) are typically more tolerant towards c&d than their individual free cells counterparts, and survival of pathogens such as listeria monocytogenes may be affected by interspecies interactions within biofilms. in this study, pseudomonas and acin ...201728667108
a novel programmable lysozyme-based lysis system in pseudomonas putida for biopolymer production.cell lysis is crucial for the microbial production of industrial fatty acids, proteins, biofuels, and biopolymers. in this work, we developed a novel programmable lysis system based on the heterologous expression of lysozyme. the inducible lytic system was tested in two gram-negative bacterial strains, namely escherichia coli and pseudomonas putida kt2440. before induction, the lytic system did not significantly arrest essential physiological parameters in the recombinant e. coli (ecpi) and p. p ...201728663596
the novel pseudomonas putida plasmid p12969-2 harbors an in127-carrying multidrug-resistant region.this study aims to characterize a multidrug-resistant (mdr) plasmid p12969-2 coexistent with the previously reported one p12969-dim in clinical pseudomonas putida.201728660784
impact of the composition of the bacterial population and additional carbon source on the pathway and kinetics of degradation of endosulfan isomers.abiotic and bacterial degradation is presented for the two isomers α- and β- of the organochlorine pesticide endosulfan, denoted as es-1 and es-2, respectively. biodegradation studies were conducted with two indigenous species pseudomonas putida (p. putida) and rhodococcus sp. both es isomers rapidly hydrolyzed in water at ph ≥ 7 but the hydrolysis was inhibited in the presence of biomass. the pesticide partitioned onto the biomass making it unavailable for abiotic hydrolytic reaction. spontaneo ...201728657620
functional role of lanthanides in enzymatic activity and transcriptional regulation of pyrroloquinoline quinone-dependent alcohol dehydrogenases in pseudomonas putida kt2440.the oxidation of alcohols and aldehydes is crucial for detoxification and efficient catabolism of various volatile organic compounds (vocs). thus, many gram-negative bacteria have evolved periplasmic oxidation systems based on pyrroloquinoline quinone-dependent alcohol dehydrogenases (pqq-adhs) that are often functionally redundant. here we report the first description and characterization of a lanthanide-dependent pqq-adh (pedh) in a nonmethylotrophic bacterium based on the use of purified enzy ...201728655819
multiple pseudomonas species secrete exolysin-like toxins and provoke caspase-1-dependent macrophage death.pathogenic bacteria secrete protein toxins that provoke apoptosis or necrosis of eukaryotic cells. here, we developed a live-imaging method, based on incorporation of a dna-intercalating dye into membrane-damaged host cells, to study the kinetics of primary bone marrow-derived macrophages (bmdms) mortality induced by opportunistic pathogen pseudomonas aeruginosa expressing either type iii secretion system (t3ss) toxins or the pore-forming toxin, exolysin (exla). we found that exla promotes the a ...201728654176
microbial culturomics application for global health: noncontiguous finished genome sequence and description of pseudomonas massiliensis strain cb-1(t) sp. nov. in brazil.culturomics is a new postgenomics field that explores the microbial diversity of the human gut coupled with taxono-genomic strategy. culturomics, and the microbiome science more generally, are anticipated to transform global health diagnostics and inform the ways in which gut microbial diversity contributes to human health and disease, and by extension, to personalized medicine. using culturomics, we report in this study the description of strain cb1(t) ( = csur p1334 = dsm 29075), a new species ...201728650741
erratum to: anoxic metabolism and biochemical production in pseudomonas putida f1 driven by a bioelectrochemical system.[this corrects the article doi: 10.1186/s13068-016-0452-y.].201728649274
membrane alterations in pseudomonas putida f1 exposed to nanoscale zerovalent iron: effects of short-term and repetitive nzvi exposure.in this study, we report the effect of the commercial nanoscale zerovalent iron (nzvi) on environmental bacteria, emphasizing the importance of nzvi-bacterial membrane interaction on nzvi toxicity as well as the adaptability of bacteria to nzvi. exposure of pseudomonas putida f1 to 0.1, 1.0, and 5.0 g/l of nzvi caused the reduction in colony forming units (cfus) substantially for almost 3 orders of magnitude. however, a rebound in the cell number was observed after the prolonged exposure except ...201728648065
in vivo and in vitro depolymerizations of intracellular medium-chain-length poly-3-hydroxyalkanoates produced by pseudomonas putida bet001.in vivo and in vitro depolymerizations of intracellular medium-chain-length poly-3-hydroxyalkanoates (mcl-pha) in pseudomonas putida bet001 grown on lauric acid was studied. both processes were studied under optimum conditions for mcl-pha depolymerization viz. 0.2 m tris-hcl buffer, ph 9, ionic strength (i) = 0.2 m at 30°c. for in vitro depolymerization studies, cell-free system was obtained from lysing bacterial cells suspension by ultrasonication at optimum conditions (frequency 37 khz, 30% of ...201728635367
insights into the genomic plasticity of pseudomonas putida kf715, a strain with unique biphenyl-utilizing activity and genome instability properties.pseudomonas putida kf715 exhibits unique properties in both catabolic activity and genome plasticity. our previous studies revealed that the dna region containing biphenyl and salycilate metabolism gene clusters (termed the bph-sal element) was frequently deleted and transferred by conjugation to closely related p. putida strains. in this study, we first determined the complete nucleotide sequence of the kf715 genome. next, to determine the underlying cause of genome plasticity in kf715, we comp ...201728631340
structural characterization of pyoverdines produced by pseudomonas putida kt2440 and pseudomonas taiwanensis vlb120.the previously unknown sequences of several pyoverdines (pvd) produced by a biotechnologically-relevant bacterium, namely, pseudomonas taiwanensis vlb120, were characterized by high performance liquid chromatography (hplc)-high resolution mass spectrometry (hrms). the same structural characterization scheme was checked before by analysis of pseudomonas sp. putida kt2440 samples with known pvds. a new sample preparation strategy based on solid-phase extraction was developed, requiring significant ...201728631237
metabolic value chemoattractants are preferentially recognized at broad ligand range chemoreceptor of pseudomonas putida kt2440.bacteria have evolved a wide range of chemoreceptors with different ligand specificities. typically, chemoreceptors bind ligands with elevated specificity and ligands serve as growth substrates. however, there is a chemoreceptor family that has a broad ligand specificity including many compounds that are not of metabolic value. to advance the understanding of this family, we have used the pcay_pp (pp2643) chemoreceptor of pseudomonas putida kt2440 as a model. using isothermal titration calorimet ...201728620365
de novo biosynthesis of glutarate via α-keto acid carbon chain extension and decarboxylation pathway in escherichia coli.microbial based bioplastics are promising alternatives to petroleum based synthetic plastics due to their renewability and economic feasibility. glutarate is one of the most potential building blocks for bioplastics. the recent biosynthetic routes for glutarate were mostly based on the l-lysine degradation pathway from pseudomonas putida that required lysine either by feeding or lysine overproduction via genetic manipulations. herein, we established a novel glutarate biosynthetic pathway by inco ...201728618222
igm-mediated autoimmune responses to oxidative specific epitopes, but not nitrosylated adducts, are significantly decreased in pregnancy: association with bacterial translocation, perinatal and lifetime major depression and the tryptophan catabolite (trycat) pathway.immunoglubulin (ig)m responses directed to oxidative specific epitopes (oses) and nitric oxide (no)-adducts are significantly associated with major depression and physio-somatic symptoms. end of term serum igm responses to oses and no-adducts were assayed in pregnant women with (n = 24) and without prenatal depression (n = 25) as well as in 24 non-pregnant women. associations of igm/iga responses to gram-negative gut commensal bacteria (leaky gut index) and iga/igm responses to tryptophan catabo ...201728600633
benzoate transport in pseudomonas putida csv86.pseudomonas putida strain csv86 metabolizes variety of aromatic compounds as the sole carbon source. genome analysis revealed the presence of genes encoding putative transporters for benzoate, p-hydroxybenzoate, phenylacetate, p-hydroxyphenylacetate and vanillate. bioinformatic analysis revealed that benzoate transport and metabolism genes are clustered at the ben locus as benk-cata-bene-benf. protein topology prediction suggests that benk (aromatic acid-h+ symporter of major facilitator superfa ...201728591829
interspecies cross-talk between co-cultured pseudomonas putida and escherichia coli.pseudomonas putida and escherichia coli are ubiquitous microorganisms that can be isolated from soil rhizosphere, the surface of vegetables, fresh waters and wastewaters - environments in which they likely co-exist. despite this, the potential interactions between these microbes have not been studied in detail. to analyse these interactions, we carried out rna-seq transcriptomic analysis of these microbes as monocultures and as co-cultures. our results show that co-culture of these microbes sign ...201728585781
influence of endophytic root bacteria on the growth, cadmium tolerance and uptake of switchgrass (panicum virgatum l.).this study aimed to evaluate the effect of five cadmium-tolerant endophytic plant growth-promoting bacteria (pgpb) strains on the biomass and cadmium tolerance, and accumulation of the bioenergy plant switchgrass (panicum virgatum l.) under cadmium (cd)-contaminated conditions.201728581636
production of 5-aminovaleric acid in recombinant corynebacterium glutamicum strains from a miscanthus hydrolysate solution prepared by a newly developed miscanthus hydrolysis process.this study examined nine expired industrial corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-ava. c. glutamicum kctc 1857 exhibiting the highest lysine production was transformed with either original pseudomonas putida davba genes, encoding the 5-ava biosynthesis pathway, or c. glutamicum codon-optimized davba genes. c. glutamicum kctc 1857 expressing the original genes had superior cell viability and 5-ava production capability compared to th ...201728579174
bioprocess engineering to produce 9-(nonanoyloxy) nonanoic acid by a recombinant corynebacterium glutamicum-based biocatalyst.here, corynebacterium glutamicum atcc13032 expressing baeyer-villiger monooxygenase from pseudomonas putida kt2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. diverse parameters including cultivation and reaction temperatures, type of detergent, and ph were found to improve biotransformation efficiency. the optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant c. glutami ...201728567672
synthetic biology of polyhydroxyalkanoates (pha).microbial polyhydroxyalkanoates (pha) are a family of biodegradable and biocompatible polyesters which have been extensively studied using synthetic biology and metabolic engineering methods for improving production and for widening its diversity. synthetic biology has allowed pha to become composition controllable random copolymers, homopolymers, and block copolymers. recent developments showed that it is possible to establish a microbial platform for producing not only random copolymers with c ...201728567487
characterization and genome analysis of a nicotine and nicotinic acid-degrading strain pseudomonas putida jq581 isolated from marine.the presence of nicotine and nicotinic acid (na) in the marine environment has caused great harm to human health and the natural environment. therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. in this study, a nicotine and na-degrading bacterium-strain jq581-was isolated from sediment from the east china sea and identified as a member of pseudomonas putida based on morphology, physio-biochemical characteristics, and 16s rdna ...201728561771
first detection of vim-2 metallo-β-lactamase-producing pseudomonas putida in blattella germanica cockroaches in an algerian hospital. 201728559273
identification of two secondary acyltransferases of lipid a in pseudomonas putida kt2442.identify the secondary acyltransferases of lipid a in pseudomonas putida.201728557109
structural studies of the o-specific polysaccharide from detergent degrading bacteria pseudomonas putida tsh-18.an o-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of bacteria pseudomonas putida tsh-18, capable of degrading non-ionogenic technical detergents. the polysaccharide was found to contain a rarely occurring sugar derivative 4,6-dideoxy-4-[(r)-3-hydroxybutanoylamino]-d-galactose [d-fucp4n(rhb)]. sugar and methylation analyses, smith degradation, solvolysis with cf3co2h, and (1)h and (13)c nmr spectroscopy enabled elucidation of the following structure of t ...201728554122
preparation and characterization of monodisperse microcapsules with alginate and bentonite via external gelation technique encapsulating pseudomonas putida rs-198.this paper evaluated the external gelation technique for preparing microcapsules. the microcapsules were consisted of pseudomonas putida rs-198 (rs-198) core and sodium alginate (naalg)-bentonite (bent) shell. different emulsification rotation speeds and core/shell ratios were used to prepare the microcapsules of each formulation. the near-spherical microcapsules were monodisperse with a mean diameter of 25-100 μm and wrinkled surfaces. fourier transform infrared spectrophotometry (ftir) and the ...201728541818
mqsr/mqsa toxin/antitoxin system regulates persistence and biofilm formation in pseudomonas putida kt2440.bacterial toxin/antitoxin (ta) systems have received increasing attention due to their prevalence, diverse structures, and important physiological functions. in this study, we identified and characterized a type ii ta system in a soil bacterium pseudomonas putida kt2440. this ta system belongs to the mqsr/mqsa family. we found that pp_4205 (mqsr) greatly inhibits cell growth in p. putida kt2440 and escherichia coli, the antitoxin pp_4204 (mqsa) neutralizes the toxicity of the toxin mqsr, and the ...201728536573
continuous supply of glucose and glycerol enhances biotransformation of ricinoleic acid to (e)-11-(heptanoyloxy) undec-9-enoic acid in recombinant escherichia coli.this study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (e)-11-(heptanoyloxy) undec-9-enoic acid (11-houa), a key intermediate of brassylic acid, by recombinant escherichia coli overexpressing an alcohol dehydrogenase from micrococcus luteus and a baeyer-villiger monooxygenase from pseudomonas putida kt2440. feeding of glucose or glycerol facilitated both the preparation of high-density cell biocatalyst and supply ...201728536060
interaction of gram-positive and gram-negative bacteria with ceramic nanomaterials obtained by combustion synthesis – adsorption and cytotoxicity studies.this paper presents the interactions of gram-positive (staphylococcus aureus) and gram-negative (pseudomonas putida) bacteria with ceramic materials obtained by combustion synthesis. these studies were conducted based on an analysis of the adsorption of bacteria onto aggregates of ceramic materials in an aqueous suspension. the materials used in the studies were of a nanostructured nature and consisted mainly of carbides: silicon carbide (sic) in the form of nanofibers (nfs) and nanorods (nrs), ...201628517917
flen and fleq play a synergistic role in regulating lapa and bcs operons in pseudomonas putida kt2440.flen generally functions as an antagonist of fleq in regulating flagellar genes and biofilm matrix related genes in pseudomonas aeruginosa. here, we found that in pseudomonas putida kt2440, flen and fleq play a synergistic role in regulating two biofilm matrix coding operons, lapa and bcs. flen deletion decreased the transcription of lapa and increased the transcription of bcs operon, and the same trend was observed in fleq deletion mutant before. in vitro experiments showed that flen promoted t ...201728517238
new insights into the regulation of cell-surface signaling activity acquired from a mutagenesis screen of the pseudomonas putida iuty sigma/anti-sigma factor.cell-surface signaling (css) is a signal transfer system that allows gram-negative bacteria to detect environmental signals and generate a cytosolic response. these systems are composed of an outer membrane receptor that senses the inducing signal, an extracytoplasmic function sigma factor (σ(ecf)) that targets the cytosolic response by modifying gene expression and a cytoplasmic membrane anti-sigma factor that keeps the σ(ecf) in an inactive state in the absence of the signal and transduces its ...201728512454
encapsulated pseudomonas putida for phenol biodegradation: use of a structural membrane for construction of a well-organized confined particle.phenols are toxic byproducts from a wide range of industry sectors. if not treated, they form effluents that are very hazardous to the environment. this study presents the use of a pseudomonas putida f1 culture encapsulated within a confined environment particle as an efficient technique for phenol biodegradation. the innovative encapsulation technique method, named the "small bioreactor platform" (sbp) technology, enables the use of a microfiltration membrane constructed as a physical barrier f ...201728505532
new prodigiosin derivatives obtained by mutasynthesis in pseudomonas putida.the deeply red-colored natural compound prodigiosin is a representative of the prodiginine alkaloid family, which possesses bioactivities as antimicrobial, antitumor, and antimalarial agents. various bacteria including the opportunistic human pathogen serratia marcescens and different members of the streptomycetaceae and pseudoalteromonadaceae produce prodiginines. in addition, these microbes generally accumulate many structurally related alkaloids making efficient prodiginine synthesis and puri ...201728505410
controlling the regioselectivity of baeyer-villiger monooxygenases by mutation of active-site residues.baeyer-villiger monooxygenase (bvmo)-mediated regiodivergent conversions of asymmetric ketones can lead to the formation of "normal" or "abnormal" lactones. in a previous study, we were able to change the regioselectivity of a bvmo by mutation of the active-site residues to smaller amino acids, which thus created more space. in this study, we demonstrate that this method can also be used for other bvmo/substrate combinations. we investigated the regioselectivity of 2-oxo-δ(3) -4,5,5-trimethylcyc ...201728504873
medium-chain-length polyhydroxyalkanoates synthesis by pseudomonas putida kt2440 rela/spot mutant: bioprocess characterization and transcriptome analysis.pseudomonas putida kt2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-phas) production using various substrates. however, despite many studies conducted on p. putida kt2440 strain, the molecular mechanisms of leading to mcl-phas synthesis in reaction to environmental stimuli are still not clear. the rearrangement of the metabolism in response to environmental stress could be controlled by stringent response that modulates the transcription of many genes i ...201728497290
characterisation of a 3-hydroxypropionic acid-inducible system from pseudomonas putida for orthogonal gene expression control in escherichia coli and cupriavidus necator.3-hydroxypropionic acid (3-hp) is an important platform chemical used as a precursor for production of added-value compounds such as acrylic acid. metabolically engineered yeast, escherichia coli, cyanobacteria and other microorganisms have been developed for the biosynthesis of 3-hp. attempts to overproduce this compound in recombinant pseudomonas denitrificans revealed that 3-hp is consumed by this microorganism using the catabolic enzymes encoded by genes hpdh, hbdh and mmsa. 3-hp-inducible s ...201728496205
the comparison of five low cost liquid formulations to preserve two phosphate solubilizing bacteria from the genera pseudomonas and pantoea.phosphorus is one of the low bioavailable macroelements. use of microorganisms in biofertilizers could release phosphorus from insoluble compounds. pseudomonas putida p13 and pantoea agglomerans p5 are well recognized for application as phosphate solubilizing bioinoculants and are used as solid carrier based. liquid bioinoculants are preferred for economizing production process and longer shelf-life.201628491248
arbuscular mycorrhizal fungi and pseudomonas in reduce drought stress damage in flax (linum usitatissimum l.): a field study.drought stress, which is one of the most serious world environmental threats to crop production, might be compensated by some free living and symbiotic soil microorganisms. the physiological response of flax plants to inoculation with two species of arbuscular mycorrhizal (am) fungi (funneliformis mosseae or rhizophagus intraradices) and a phosphate solubilizing bacterium (pseudomonas putida p13; psb) was evaluated under different irrigation regimes (irrigation after 60, 120, and 180 mm of evapo ...201728488060
ros mediated high anti-bacterial efficacy of strain tolerant layered phase pure nano-calcium hydroxide.the present work provides the first ever report on extraordinarily high antibacterial efficacy of phase pure micro-layered calcium hydroxide nanoparticles (lchnps) even under dark condition. the lchnps synthesized especially in aqueous medium by a simple, inexpensive method show adequate mechanical properties along with the presence of a unique strain tolerant behaviour. the lchnps are characterized by ftir, raman spectroscopy, xrd, rietveld analysis, fe-sem, tem, tg-dta, surface area, particle ...201728477521
isotope fractionation associated with the simultaneous biodegradation of multiple nitrophenol isomers by pseudomonas putida b2.quantifying the extent of biodegradation of nitroaromatic compounds (nacs) in contaminated soils and sediments is challenging because of competing oxidative and reductive reaction pathways. we have previously shown that the stable isotope fractionation of nacs reveals the routes of degradation even if it is simultaneously caused by different bacteria. however, it is unclear whether compound-specific isotope analysis (csia) can be applied in situations where multiple pollutants are biodegraded by ...201728470308
nosocomial pseudomonas putida meningitis: a case report and literature review.we report a case of pseudomonas putida meningitis in a 26-year-old nepalese man who was admitted to hamad general hospital with epidermoid cyst for drainage. ommaya reservoir was placed into the cyst for drainage and externalventricular drainage (evd) was performed. after four days, the patient was transferred to the ward in stable condition. his weakness resolved partially and headache severity decreased. after three days, the patient developed fever and headache severity increased with deterio ...201728439390
presence of vim-positive pseudomonas species in chickens and their surrounding environment.metallo-β-lactamase gene blavim was identified on the chromosome of four pseudomonas sp. isolates from a chicken farm, including one pseudomonas aeruginosa isolate from a swallow (yanornis martini), one pseudomonas putida isolate from a fly, and two p. putida isolates from chickens. the four isolates shared two variants of blavim-carrying genomic contexts that resemble the corresponding regions of clinical metallo-β-lactamase-producing pseudomonas spp. our study suggests that the surveillance of ...201728438943
draft genome sequence of acinetobacter johnsonii c6, an environmental isolate engaging in interspecific metabolic interactions.acinetobacter johnsonii c6 originates from creosote-polluted groundwater and performs ecological and evolutionary interactions with pseudomonas putida in biofilms. the draft genome of a. johnsonii c6 is 3.7 mbp and was shaped by mobile genetic elements. it reveals genes facilitating the biodegradation of aromatic hydrocarbons and resistance to antimicrobials and metals.201728428290
engineering a promiscuous tautomerase into a more efficient aldolase for self-condensations of linear aliphatic aldehydes.the enzyme 4-oxalocrotonate tautomerase (4-ot) from pseudomonas putida mt-2 takes part in a catabolic pathway for aromatic hydrocarbons, where it catalyzes the conversion of 2hydroxyhexa-2,4-dienedioate into 2-oxohexa-3-enedioate. this tautomerase can also promiscuously catalyze carbon-carbon bond-forming reactions, including various types of aldol reactions, by using its amino-terminal proline as a key catalytic residue. here, we used systematic mutagenesis to identify two hotspots in 4-ot (met ...201728426139
suppression of enteric bacteria by bacteriophages: importance of phage polyvalence in the presence of soil bacteria.bacteriophages are widely recognized for their importance in microbial ecology and bacterial control. however, little is known about how phage polyvalence (i.e., broad host range) affects bacterial suppression and interspecies competition in environments harboring enteric pathogens and soil bacteria. here we compare the efficacy of polyvalent phage pef1 versus coliphage t4 in suppressing a model enteric bacterium (e. coli k-12) in mixtures with soil bacteria (pseudomonas putida f1 and bacillus s ...201728414441
regulation of solvent tolerance in pseudomonas putida s12 mediated by mobile elements.organic solvent-tolerant bacteria are outstanding and versatile hosts for the bio-based production of a broad range of generally toxic aromatic compounds. the energetically costly solvent tolerance mechanisms are subject to multiple levels of regulation, involving among other mobile genetic elements. the genome of the solvent-tolerant pseudomonas putida s12 contains many such mobile elements that play a major role in the regulation and adaptation to various stress conditions, including the regul ...201728401676
delignification and enhanced gas release from soil containing lignocellulose by treatment with bacterial lignin degraders.the aim of the study was to isolate bacterial lignin-degrading bacteria from municipal solid waste soil, and to investigate whether they could be used to delignify lignocellulose-containing soil, and enhance methane release.201728393443
production of polyhydroxyalkanoates (pha) using sludge from different wastewater treatment processes and the potential for medical and pharmaceutical applications.in this study, seven strains of bacteria with polyhydroxyalkanoates (pha)-producing ability (i.e., bacillus cereus, pseudomonas putida, bacillus pumilus, pseudomona-huttiensis, yersinia-frederiksenii, aeromonas ichthiosmia and sphingopyxis terrae) were isolated from various waste treatment plants in hong kong. simultaneous wastewater treatment and pha accumulation were successfully achieved in the bioreactors using isolated bacteria from different sludges. at the organic loading less than 13000 ...201728387154
bacteria can proliferate in thawed cryoprecipitate stored at room temperature for longer than 4 h.although key coagulation factor activities are maintained in thawed cryoprecipitate stored for up to 24 h at ambient temperature, several jurisdictions limit such storage to 4-6 h. here, we separately spiked thawed cryoprecipitate units with four bacterial strains: staphylococcus epidermidis, serratia liquefaciens, pseudomonas putida and pseudomonas aeruginosa. no strains grew in the first 4 h of storage, but by 24 h, three of four exhibited up to 1000-fold proliferation. pathogen inactivation t ...201728382701
seed vectored endophytic bacteria modulate development of rice seedlings.the aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens.201728375579
the ability of consortium wastewater protozoan and bacterial species to remove cod in the presence of nanomaterials under varying ph conditions.the aim of this study was to ascertain the survival limit and capability of commonly found wastewater protozoan (aspidisca sp, trachelophyllum sp and peranema sp) and bacterial (bacillus licheniformis, brevibacillus laterosporus and pseudomonas putida) species to remove cod while exposed to commercial nanomaterials under varying ph conditions. the experimental study was carried out in modified mixed liquor media adjusted to various ph levels (ph 2, 7 and 10) and a comparative study was performed ...201728368692
highly efficient simultaneous adsorption and biodegradation of a highly-concentrated anionic dye by a high-surface-area carbon-based biocomposite.mesoporous high-surface-area activated carbon (mhsa-ac), which has a honeycomb structure, was produced from coconut shells by simultaneous chemical and physical activation and used for the rapid adsorption of an anionic dye, namely acid orange 10 (ao10), from water. owing to its porosity and high brunauer-emmett-teller surface area (2283.91 m(2)g(-1)), mhsa-ac is a highly efficient adsorbent. it also has good biocompatibility and is a good immobilization carrier; the grooves on the mhsa-ac surfa ...201728365499
draft genome sequence of pseudomonas putida strain gm4fr, an endophytic bacterium isolated from festuca rubra l.pseudomonas putida gm4fr is an endophytic bacterium isolated from aerial plant tissues of festuca rubra l. functional annotation of the draft genome (7.1 mb) revealed 6,272 predicted protein-encoding genes. the genome provides insights into the biocontrol and plant growth-promoting potential of p. putida gm4fr.201728360162
deconvolution of gene expression noise into spatial dynamics of transcription factor-promoter interplay.gene expression noise is not only the mere consequence of stochasticity, but also a signal that reflects the upstream physical dynamics of the cognate molecular machinery. soil bacteria facing recalcitrant pollutants exploit noise of catabolic promoters to deploy beneficial phenotypes such as metabolic bet-hedging and/or division of biochemical labour. although the role of upstream promoter-regulator interplay in the origin of this noise is little understood, its specifications are probably ciph ...201728355056
use of response surface method for maximizing the production of arginine deiminase by pseudomonas putida.statistically designed experiments were used to optimize the production of arginine deiminase (adi) by pseudomonas putida kt2440 in batch culture. a plackett-burman design involving eleven factors showed that adi production was most influenced by the initial ph and the initial concentrations of glucose and yeast extract. a central composite experimental design showed that the optimal values of these factors were 8.0, 10 g/l and 12.5 g/l, respectively. the other components of the optimal culture ...201628352521
indole-induced activities of β-lactamase and efflux pump confer ampicillin resistance in pseudomonas putida kt2440.indole, which is widespread in microbial communities, has received attention because of its effects on bacterial physiology. pseudomonas putida and pseudomonas aeruginosa can acquire ampicillin (amp) resistance during growth on indole-amp agar. transcriptome, mutant, and inhibitor studies have suggested that amp resistance induced by indole can be attributed to increased gene expression of ttgab encoding two genes of rnd-type multidrug efflux operons and an ampc encoding β-lactamase. expression, ...201728352264
the n-acetylmuramic acid 6-phosphate phosphatase mupp completes the pseudomonas peptidoglycan recycling pathway leading to intrinsic fosfomycin resistance.bacterial cells are encased in and stabilized by a netlike peptidoglycan (pgn) cell wall that undergoes turnover during bacterial growth. pgn turnover fragments are frequently salvaged by the cells via a pathway referred to as pgn recycling. two different routes for the recycling of the cell wall sugar n-acetylmuramic acid (murnac) have been recognized in bacteria. in escherichia coli and related enterobacteria, as well as in most gram-positive bacteria, murnac is recovered via a catabolic route ...201728351914
metabolic engineering to expand the substrate spectrum of pseudomonas putida toward sucrose.sucrose is an important disaccharide used as a substrate in many industrial applications. it is a major component of molasses, a cheap by-product of the sugar industry. unfortunately, not all industrially relevant organisms, among them pseudomonas putida, are capable of metabolizing sucrose. we chose a metabolic engineering approach to circumvent this blockage and equip p. putida with the activities necessary to consume sucrose. therefore, we constructed a pair of broad-host range mini-transposo ...201728349670
the impact of succinate trace on pww0 and ortho-cleavage pathway transcription in pseudomonas putida mt-2 during toluene biodegradation.toluene is a pollutant catabolised through the interconnected pww0 (tol) and ortho-cleavage pathways of pseudomonas putida mt-2, while upon succinate and toluene mixtures introduction in batch cultures grown on m9 medium, succinate was previously reported as non-repressing. the effect of a 40 times lower succinate concentration, as compared to literature values, was explored through systematic real-time qpcr monitoring of transcriptional kinetics of the key tol pu, pm and ortho-cleavage pbenr, p ...201728347959
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