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simultaneous identification of dna and rna viruses present in pig faeces using process-controlled deep sequencing.animal faeces comprise a community of many different microorganisms including bacteria and viruses. only scarce information is available about the diversity of viruses present in the faeces of pigs. here we describe a protocol, which was optimized for the purification of the total fraction of viral particles from pig faeces. the genomes of the purified dna and rna viruses were simultaneously amplified by pcr and subjected to deep sequencing followed by bioinformatic analyses. the efficiency of t ...201222514648
discovery of a novel circular single-stranded dna virus from porcine faeces.a large number of novel single-stranded dna (ssdna) viruses have been characterised from various environmental sources in the last 5 years. the bulk of these have been from faecal sources, and faecal sampling is an ideal non-invasive pathogen sampling method. we characterised a novel ssdna from a porcine faecal sample from cass basin of the south island of new zealand. the novel viral genome has two large open reading frames (orfs), which are bidirectionally transcribed and separated by intergen ...201322972681
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