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emended description of the genus polynucleobacter and the species polynucleobacter necessarius and proposal of two subspecies, p. necessarius subsp. necessarius subsp. nov. and p. necessarius subsp. asymbioticus subsp. nov.heckmann and schmidt described the genus polynucleobacter for bacterial endosymbionts of freshwater ciliates affiliated with the genus euplotes, and the species polynucleobacter necessarius for obligate endosymbionts living in the cytoplasm of euplotes aediculatus. pure cultures of the type strain could not be established due to the obligate nature of the symbiotic relationship between the endosymbionts and their hosts. therefore, polynucleobacter necessarius is one of a few bacterial species wi ...200919567561
reclassification of four polynucleobacter necessarius strains as representatives of polynucleobacter asymbioticus comb. nov., polynucleobacter duraquae sp. nov., polynucleobacter yangtzensis sp. nov. and polynucleobacter sinensis sp. nov., and emended description of polynucleobacter necessarius.genome comparisons based on average nucleotide identity (ani) values of four strains currently classified as polynucleobacter necessarius subsp. asymbioticus resulted in ani values of 75.7-78.4 %, suggesting that each of those strains represents a separate species. the species p. necessarius was proposed by heckmann and schmidt in 1987 to accommodate obligate endosymbionts of ciliates affiliated with the genus euplotes. the required revision of this species is, however, hampered by the fact, tha ...201627064460
microdiversification of a pelagic polynucleobacter species is mainly driven by acquisition of genomic islands from a partially interspecific gene pool.microdiversification of a planktonic freshwater bacterium was studied by comparing 37 polynucleobacter asymbioticus strains obtained from three geographically separated sites in the austrian alps. genome comparison of nine strains revealed a core genome of 1.8 mb, representing 81% of the average genome size. seventy-five percent of the remaining flexible genome is clustered in genomic islands (gis). twenty-four genomic positions could be identified where gis are potentially located. these positi ...201727836842
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