Publications

taxon k, a complex within the burkholderia cepacia complex, comprises at least two novel species, burkholderia contaminans sp. nov. and burkholderia lata sp. nov.	the aim of the present study was to re-examine the taxonomic position and structure of taxon k (also known as group k) within the burkholderia cepacia complex (bcc). for this purpose, a representative set of strains was examined by a traditional polyphasic taxonomic approach, by multilocus sequence typing (mlst) analysis and by analysis of available whole-genome sequences. analysis of the reca gene sequence revealed three different lineages, designated reca-i, reca-ii and reca-iii. dna-dna hybri ...	2009	19126732
production of the antifungal compound pyrrolnitrin is quorum sensing-regulated in members of the burkholderia cepacia complex.	members of the genus burkholderia are known for their ability to suppress soil-borne fungal pathogens by the production of various antibiotic compounds. in this study we investigated the role of n-acylhomoserine lactone (ahl)-dependent quorum sensing (qs) in the expression of antifungal traits. using a quorum quenching approach, that is, by heterologous expression of the bacillus sp. aiia lactonase, we show that expression of antifungal activities is ahl-dependent in the large majority of the in ...	2009	19220396
reca gene sequence and multilocus sequence typing for species-level resolution of burkholderia cepacia complex isolates.	to identify, by means of reca sequencing and multilocus sequence typing (mlst), burkholderia cepacia complex (bcc) isolates of environmental and clinical origin, which failed to be identified by reca rflp and species-specific pcr.	2009	19732325
ligand binding induces an ammonia channel in 2-amino-2-desoxyisochorismate (adic) synthase phze.	phze utilizes chorismate and glutamine to synthesize 2-amino-2-desoxyisochorismate (adic) in the first step of phenazine biosynthesis. the phze monomer contains both a chorismate-converting menaquinone, siderophore, tryptophan biosynthesis (mst) and a type 1 glutamine amidotransferase (gatase1) domain connected by a 45-residue linker. we present here the crystal structure of phze from burkholderia lata 383 in a ligand-free open and ligand-bound closed conformation at 2.9 and 2.1 å resolution, re ...	2011	21454481
trapped intermediates in crystals of the fmn-dependent oxidase phzg provide insight into the final steps of phenazine biosynthesis.	phenazines are redox-active secondary metabolites that many bacteria produce and secrete into the environment. they are broad-specificity antibiotics, but also act as virulence and survival factors in infectious diseases. phenazines are derived from chorismic acid, but important details of their biosynthesis are still unclear. for example, three two-electron oxidations seem to be necessary in the final steps of the pathway, while only one oxidase, the fmn-dependent phzg, is conserved in the phen ...	2013	23897464
the effect of cationic microbicide exposure against burkholderia cepacia complex (bcc); the use of burkholderia lata strain 383 as a model bacterium.	the extensive use of microbicides in a wide range of applications has been questioned with regard to their role in the development of bacterial resistance to antimicrobials. this study aims to measure the phenotypic and genotypic changes in burkholderia lata strain 383 exposed to chlorhexidine gluconate (chg) and benzalkonium chloride (bzc), two commonly used cationic microbicides.	2013	23910391