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bellilinea caldifistulae gen. nov., sp. nov. and longilinea arvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria of the phylum chloroflexi isolated from methanogenic propionate-degrading consortia.thermophilic (strain gomi-1(t)) and mesophilic (strain kome-1(t)) strains were isolated from two different cultures of propionate-degrading consortia obtained from thermophilic digester sludge and rice paddy soil, respectively. the two strains were non-spore-forming, non-motile and gram-negative. both strains were obligately anaerobic micro-organisms, showing multicellular filamentous morphotypes more than 100 mum in length. the cell width for strain gomi-1(t) was 0.2-0.4 mum and that of strain ...200717911301
cultivation of uncultured chloroflexi subphyla: significance and ecophysiology of formerly uncultured chloroflexi 'subphylum i' with natural and biotechnological relevance.cultivation-independent molecular surveys have shown members of the bacterial phylum chloroflexi to be ubiquitous in various natural and artificial ecosystems. among the subphylum-level taxa of the chloroflexi known to date, the formerly uncultured 'subphylum i' had well been recognized as a typical group that contains a number of environmental gene clones with no culture representatives. in order to reveal their ecophysiology, attempts were made over the past decade to domesticate them into lab ...200921566375
isolation and characterization of thermanaerothrix daxensis gen. nov., sp. nov., a thermophilic anaerobic bacterium pertaining to the phylum "chloroflexi", isolated from a deep hot aquifer in the aquitaine basin.a new strictly anaerobic thermophilic multicellular filamentous bacterium (0.2-0.3μm×>100μm), designated gns-1(t), was isolated from a deep hot aquifer in france. it was non-motile, and stained gram-negative. optimal growth was observed at 65°c, ph 7.0, and 2gl(-1) of nacl. strain gns-1(t) was chemoorganotrophic fermenting ribose, glucose, galactose, arabinose, fructose, mannose, maltose, sucrose, xylose, raffinose, pyruvate, and xylan. yeast extract was required for growth. the end products of ...201121621938
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