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association of anurans with pathogenic fungi.in a study of 450 amazonian anurans, we isolated yeasts and yeast-like fungi from 54 animals (bufo granulosus, b. marinus, dendrophrynyscus sp., hyla geographica, h. lanciformes, ololygon rubra, adenomera hylaedactyla, eleutherodactylus fenestratus, leptodactylus fuscus, l. ocellatus, l. pentadactylus). the internal organs of these animals did not show any macroscopic anomaly nor histopathology. we recovered 105 fungal isolates from the anuran liver, lung, kidney, spleen, heart and gonad. the is ...19854069190
unusual intra-individual karyotypical variation and evidence of cryptic species in amazonian populations of pristimantis (anura, terrarana).we report a cytogenetic analysis of three pristimantis fenestratus frog populations of the amazon region in brazil. the specimens were sampled in borba and manaus, amazonas state, and in rio branco, acre state. they were analyzed using giemsa, silver staining, c-banding and fish with rdna probes. the karyotypes of the three populations revealed 2n = 34 chromosomes, but they differed in the number and position of ag-nors and in the heterochromatin pattern as well. the nor was located on the pairs ...200919765094
mitogenome assembly from genomic multiplex libraries: comparison of strategies and novel mitogenomes for five species of frogs.next-generation sequencing continues to revolutionize biodiversity studies by generating unprecedented amounts of dna sequence data for comparative genomic analysis. however, these data are produced as millions or billions of short reads of variable quality that cannot be directly applied in comparative analyses, creating a demand for methods to facilitate assembly. we optimized an in silico strategy to efficiently reconstruct high-quality mitochondrial genomes directly from genomic reads. we te ...201626607054
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