Publications

analysis of bacterial communities in soil by use of denaturing gradient gel electrophoresis and clone libraries, as influenced by different reverse primers.	to assess soil bacterial diversity, pcr systems consisting of several slightly different reverse primers together with forward primer f968-gc were used along with subsequent denaturing gradient gel electrophoresis (dgge) or clone library analyses. in this study, a set of 13 previously used and novel reverse primers was tested with the canonical forward primer as to the dgge fingerprints obtained from grassland soil. analysis of these dgge profiles by gelcompar showed that they all fell into two ...	2008	18310425
bacterial diversity in a mine water treatment plant.	we investigated the microbial community in a pilot plant for treatment of acid mine water by biological ferrous iron oxidation using clone library analysis and calculated statistical parameters for further characterization. the microbial community in the plant was conspicuously dominated by a group of betaproteobacteria affiliated with "ferribacter polymyxa".	2009	19047391
matrix composition and community structure analysis of a novel bacterial pyrite leaching community.	here we describe a novel bacterial community that is embedded in a matrix of carbohydrates and bio/geochemical products of pyrite (fes(2)) oxidation. this community grows in stalactite-like structures--snottites--on the ceiling of an abandoned pyrite mine at ph values of 2.2-2.6. the aqueous phase in the matrix contains 200 mm of sulfate and total iron concentrations of 60 mm. micro-x-ray diffraction analysis showed that jarosite [(k,na,h(3)o)fe(3)(so(4))(2)(oh)(6)] is the major mineral embedded ...	2009	19519871
seasonal and spatial variations in microbial community structure and diversity in the acid stream draining across an ongoing surface mining site.	this study examined the microbial community in an acidic stream draining across the yun-fu pyrite mine (guangdong, china), where extremely acidic mine water is a persistent feature due to the intensive surface mining activities. analysis of terminal restriction fragment length polymorphism (trflp) of 16s rrna gene sequences showed that microbial populations varied spatially and seasonally and correlated with geochemical and physical conditions. after the stream moves from underground to the surf ...	2009	19678846
population dynamics of iron-oxidizing communities in pilot plants for the treatment of acid mine waters.	the iron-oxidizing microbial community in two pilot plants for the treatment of acid mine water was monitored to investigate the influence of different process parameters such as ph, iron concentration, and retention time on the stability of the system to evaluate the applicability of this treatment technology on an industrial scale. the dynamics of the microbial populations were followed using t-rflp (terminal restriction fragment length polymorphism) over a period of several months. for a more ...	2009	19746704
visualizing acidophilic microorganisms in biofilm communities using acid stable fluorescence dyes.	bacteria in acidophilic biofilm communities, i.e. acid streamers and snottites, obtained from a subsurface mine in königstein were visualized by fluorescence microscopy using four new fluorescent dyes (dy-601xl, v07-04118, v07-04146, dy-613). the ph of the bulk solution in which these bacteria thrive was ph 2.6 to 2.9. the new fluorescent dyes were all able to clearly stain and microscopically visualize in-situ the bacteria within the biofilm community without changing ph or background ion conce ...	2010	20386966
application of a depositional facies model to an acid mine drainage site.	lower red eyes is an acid mine drainage site in pennsylvania where low-ph fe(ii) oxidation has created a large, terraced iron mound downstream of an anoxic, acidic, metal-rich spring. aqueous chemistry, mineral precipitates, microbial communities, and laboratory-based fe(ii) oxidation rates for this site were analyzed in the context of a depositional facies model. depositional facies were defined as pools, terraces, or microterracettes based on cm-scale sediment morphology, irrespective of the d ...	2010	21097582
biodiversity and geochemistry of an extremely acidic, low-temperature subterranean environment sustained by chemolithotrophy.	the geochemical dynamics and composition of microbial communities within a low-temperature (ôê+8.5-¦c), long-abandoned (>ôçâ90 years) underground pyrite mine (cae coch, located in north wales) were investigated. surface water percolating through fractures in the residual pyrite ore body that forms the roof of the mine becomes extremely acidic and iron-enriched due to microbially accelerated oxidative dissolution of the sulfide mineral. water droplets on the mine roof were found to host a very li ...	2011	21382147
the influence of biofilms on the migration of uranium in acid mine drainage (amd) waters.	the uranium mine in k├ânigstein (germany) is currently in the process of being flooded. huge mass of ferrovum myxofaciens dominated biofilms are growing in the acid mine drainage (amd) water as macroscopic streamers and as stalactite-like snottites hanging from the ceiling of the galleries. microsensor measurements were performed in the amd water as well as in the biofilms from the drainage channel on-site and in the laboratory. the analytical data of the amd water was used for the thermodynamic ...	2011	21665018
schwertmannite formation adjacent to bacterial cells in a mine water treatment plant and in pure cultures of ferrovum myxofaciens.	schwertmannite has previously been found in iron- and sulfate-rich mine waters at ph 2.8-4.5. in the present study, schwertmannite (fe(8)o(8)(oh)(6)so(4)) was shown to be the major mineral in a mine water treatment plant at ph 3, in which ferrous iron is mainly oxidized by bacteria belonging to the species ferrovum myxofaciens. strain ehs6, which is closely related to the type strain of fv. myxofaciens, was isolated from the pilot plant and characterized as an acidophilic, iron-oxidizing bacteri ...	2011	21838259
A modular continuous flow reactor system for the selective bio-oxidation of iron and precipitation of schwertmannite from mine-impacted waters.	A novel modular bioremediation system which facilitates the selective removal of soluble iron from extremely acidic (pH ~2) metal-rich wastewaters by ferrous iron oxidation and selective precipitation of the ferric iron produced is described. In the first of the three modules, rapid ferrous iron oxidation was mediated by the recently-characterized iron-oxidizing autotrophic acidophile, "Ferrovum myxofaciens", which grew as long "streamers" within the reactor. Over 90% of the iron present in infl ...	2011	22197072