Publications

reclassification of roseobacter gallaeciensis ruiz-ponte et al. 1998 as phaeobacter gallaeciensis gen. nov., comb. nov., description of phaeobacter inhibens sp. nov., reclassification of ruegeria algicola (lafay et al. 1995) uchino et al. 1999 as marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera roseobacter, ruegeria and leisingera.	a heterotrophic, antibiotic-producing bacterium, strain t5(t), was isolated from the german wadden sea, located in the southern region of the north sea. sequence analysis of the 16s rrna gene of this strain demonstrated close affiliation with roseobacter gallaeciensis bs107(t) (99 % similarity), but the results of genotypic (dna-dna hybridization and dna g + c content) and phenotypic characterization revealed that strain t5(t) represents a novel species. the novel organism is strictly aerobic, g ...	2006	16738106
production of antibacterial compounds and biofilm formation by roseobacter species are influenced by culture conditions.	bacterial communities associated with marine algae are often dominated by members of the roseobacter clade, and in the present study, we describe roseobacter phenotypes that may provide this group of bacteria with selective advantages when colonizing this niche. nine of 14 members of the roseobacter clade, of which half were isolated from cultures of the dinoflagellate pfiesteria piscicida, produced antibacterial compounds. many non-roseobacter marine bacteria were inhibited by sterile filtered ...	2007	17098910
bacteria of the roseobacter clade show potential for secondary metabolite production.	members of the roseobacter clade are abundant and widespread in marine habitats and have very diverse metabolisms. production of acylated homoserine lactones (ahl) and secondary metabolites, e.g., antibiotics has been described sporadically. this prompted us to screen 22 strains of this group for production of signaling molecules, antagonistic activity against bacteria of different phylogenetic groups, and the presence of genes encoding for nonribosomal peptide synthetases (nrps) and polyketide ...	2007	17351813
phaeobacter daeponensis sp. nov., isolated from a tidal flat of the yellow sea in korea.	a gram-negative, motile and egg-shaped bacterium, strain tf-218(t), was isolated from a tidal flat at daepo beach (yellow sea), korea, and subjected to a polyphasic taxonomic analysis. strain tf-218(t) grew optimally at ph 7.0-8.0 and 37 degrees c in the presence of 2 % (w/v) nacl. it contained q-10 as the predominant ubiquinone and c(18 : 1)omega7c and 11-methyl c(18 : 1)omega7c as the major fatty acids. the major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanola ...	2007	17392219
low densities of epiphytic bacteria from the marine alga ulva australis inhibit settlement of fouling organisms.	bacteria that produce inhibitory compounds on the surface of marine algae are thought to contribute to the defense of the host plant against colonization of fouling organisms. however, the number of bacterial cells necessary to defend against fouling on the plant surface is not known. pseudoalteromonas tunicata and phaeobacter sp. strain 2.10 (formerly roseobacter gallaeciensis) are marine bacteria often found in association with the alga ulva australis and produce a range of extracellular inhib ...	2007	17965210
genetic dissection of tropodithietic acid biosynthesis by marine roseobacters.	the symbiotic association between the roseobacter silicibacter sp. strain tm1040 and the dinoflagellate pfiesteria piscicida involves bacterial chemotaxis to dinoflagellate-produced dimethylsulfoniopropionate (dmsp), dmsp demethylation, and ultimately a biofilm on the surface of the host. biofilm formation is coincident with the production of an antibiotic and a yellow-brown pigment. in this report, we demonstrate that the antibiotic is a sulfur-containing compound, tropodithietic acid (tda). us ...	2008	18192410
phaeobacter arcticus sp. nov., a psychrophilic bacterium isolated from the arctic.	a gram-negative, psychrophilic, motile, rod-shaped bacterium, designated strain 20188(t), was isolated from marine samples collected from the arctic (7 degrees 00' 24'' n 16 degrees 59' 37'' w), and was identified taxonomically by means of a polyphasic study. on the basis of 16s rrna gene sequence similarity, strain 20188(t) was closely related to members of the genera phaeobacter. 16s rrna gene sequence similarities between strain 20188(t) and the type strains of phaeobacter inhibens, phaeobact ...	2008	18523182
pelagicola litoralis gen. nov., sp. nov., isolated from coastal water in korea.	a gram-negative, strictly aerobic, non-motile, club-shaped bacterial strain, designated cl-es2(t), was isolated from coastal water from the east coast of korea. phylogenetic analysis of 16s rrna gene sequences revealed that strain cl-es2(t) was related to the genera phaeobacter (95.0-96.6% similarity to the type strains), leisingera (96.1%) and marinovum (95.6%) in the family rhodobacteraceae. however, strain cl-es2(t) did not form a robust clade with any species of the roseobacter clade, instea ...	2008	18768612
phaeobacter and ruegeria species of the roseobacter clade colonize separate niches in a danish turbot (scophthalmus maximus)-rearing farm and antagonize vibrio anguillarum under different growth conditions.	members of the roseobacter clade colonize a spanish turbot larval unit, and one isolate (phaeobacter strain 27-4) is capable of disease suppression in in vivo challenge trials. here, we demonstrate that roseobacters with antagonistic activity against vibrio anguillarum also colonize a danish turbot larval farm that relies on a very different water source (the danish fiord limfjorden as opposed to the galician atlantic ocean). phylogenetic analyses based on 16s rrna and gyrase b gene sequences re ...	2008	18952864
leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of leisingera methylohalidivorans schaefer et al. 2002, phaeobacter daeponensis yoon et al. 2007 and phaeobacter inhibens martens et al. 2006.	strain lmg 24366(t) was isolated from a marine electroactive biofilm grown on a stainless steel cathode (genova, italy) and was investigated by using a polyphasic taxonomic approach. this study demonstrated that strain lmg 24366(t) represents a novel species within the genus leisingera, which shared 98.9 % 16s rrna gene similarity with its nearest phylogenetic neighbour, leisingera methylohalidivorans. strain lmg 24366(t) grew on betaine (1 mm) as a sole carbon source, whereas no growth was obse ...	2008	19060059
real-time pcr detection and quantification of fish probiotic phaeobacter strain 27-4 and fish pathogenic vibrio in microalgae, rotifer, artemia and first feeding turbot (psetta maxima) larvae.	to develop a sybr green quantitative real-time pcr protocol enabling detection and quantification of a fish probiotic and two turbot pathogenic vibrio spp. in microcosms.	2009	19187159
phaeobacter caeruleus sp. nov., a blue-coloured, colony-forming bacterium isolated from a marine electroactive biofilm.	three isolates (lmg 24369(t), lmg 24370 and r-26156) obtained from a marine electroactive biofilm that was grown on a cathodically polarized electrode were investigated by using a polyphasic taxonomic approach. whole-cell fatty acid methyl ester and 16s rrna gene sequence analyses indicated that the isolates were members of the genus phaeobacter, class alphaproteobacteria. genotypic and phenotypic analyses demonstrated that the three isolates represent a novel species of the genus phaeobacter, f ...	2009	19406821
seohaeicola saemankumensis gen. nov., sp. nov., isolated from a tidal flat.	a gram-negative, non-motile and rod-, oval- or coccoid-shaped bacterial strain, sd-15(t), was isolated from a tidal flat of the yellow sea, korea. the novel strain, which was phylogenetically closely related to the genera phaeobacter, leisingera and marinovum, was studied using a polyphasic taxonomic approach. strain sd-15(t) grew optimally at ph 7.0-8.0 and 30 degrees c in the presence of 2 % (w/v) nacl. it contained q-10 as the predominant ubiquinone and c(18 : 1)omega7c and 11-methyl c(18 : 1 ...	2009	19625441
growth phase-dependent global protein and metabolite profiles of phaeobacter gallaeciensis strain dsm 17395, a member of the marine roseobacter-clade.	the marine heterotrophic roseobacter phaeobacter gallaeciensis dsm 17395 was grown with glucose in defined mineral medium. relative abundance changes of global protein (2-d dige) and metabolite (gc-ms) profiles were determined across five different time points of growth. in total, 215 proteins were identified and 147 metabolites detected (101 structurally identified), among which 60 proteins and 87 metabolites displayed changed abundances upon entry into stationary growth phase. glucose breakdow ...	2009	19639587
leisingera nanhaiensis sp. nov., isolated from marine sediment.	an aerobic, gram-staining-negative, motile, rod-shaped bacterium, strain nh52f(t), was isolated from a sandy sediment sample taken from the south china sea. on m2 agar medium (a complex medium), colonies were beige in colour. the isolate showed highest 16s rrna gene sequence similarities to members of the genera leisingera (96.7 % similarity), phaeobacter (95.4-96.0 %) and marinovum (94.1 %). phylogenetic analysis based on 16s rrna gene sequences indicated that strain nh52f(t) formed a distinct ...	2010	19651744
inhibitory activity of phaeobacter strains against aquaculture pathogenic bacteria.	a total of 523 bacterial strains were isolated during a 4-year period from mollusc hatcheries (flat oyster and clams) in galicia (nw spain). all of the strains were tested for their antibacterial activity against three larval pathogens (vibrio anguillarum usc-72, v. neptunius pp-145.98, and vibrio sp. pp-203). of the isolates, 52 inhibited at least one of the target strains, and 11 inhibited all of them. the main source of active strains was oyster larvae, followed by water, tank surfaces, spat, ...	2009	19784930
metabolic fluxes in the central carbon metabolism of dinoroseobacter shibae and phaeobacter gallaeciensis, two members of the marine roseobacter clade.	in the present work the central carbon metabolism of dinoroseobacter shibae and phaeobacter gallaeciensis was studied at the level of metabolic fluxes. these two strains belong to the marine roseobacter clade, a dominant bacterial group in various marine habitats, and represent surface-associated, biofilm-forming growth (p. gallaeciensis) and symbiotic growth with eukaryotic algae (d. shibae). based on information from recently sequenced genomes, a rich repertoire of pathways has been identified ...	2009	19788729
genetic tools for the investigation of roseobacter clade bacteria.	the roseobacter clade represents one of the most abundant, metabolically versatile and ecologically important bacterial groups found in marine habitats. a detailed molecular investigation of the regulatory and metabolic networks of these organisms is currently limited for many strains by missing suitable genetic tools.	2009	20021642
identification and biosynthesis of tropone derivatives and sulfur volatiles produced by bacteria of the marine roseobacter clade.	bacteria of the roseobacter clade are abundant marine bacteria and are important contributors to the global sulfur cycle. the volatiles produced by two of its members, phaeobacter gallaeciensis and oceanibulbus indolifex, were analyzed to investigate whether the released compounds are derived from sulfur metabolism, and which biosynthetic pathways are involved in their formation. both bacteria emitted different sulfides and thioesters, including new natural compounds such as s-methyl phenylethan ...	2010	20024154
pathways and substrate specificity of dmsp catabolism in marine bacteria of the roseobacter clade.	the volatiles released by phaeobacter gallaeciensis, oceanibulbus indolifex and dinoroseobacter shibae have been investigated by gc-ms, and several mesh-derived sulfur volatiles have been identified. an important sulfur source in the oceans is the algal metabolite dimethylsulfoniopropionate (dmsp). labelled [2h6]dmsp was fed to the bacteria to investigate the production of volatiles from this compound through the lysis pathway to [2h6]dimethylsulfide or the demethylation pathway to [2h3]-3-(meth ...	2010	20043308
diversity of sulfur-oxidizing bacteria in greenwater system of coastal aquaculture.	reduced sulfur compounds produced by the metabolism are the one of the major problems in aquaculture. in the present study, herbivorous fishes have been cultured as biomanipulators for secretions of slime, which enhanced the production of greenwater containing beneficial bacteria. the genes encoding soxb which is largely unique to sulfur-oxidizing bacteria (sob) due to its hydrolytic function has been targeted for examining the diversity of sob in the green water system of coastal aquaculture. n ...	2010	20069462
microfabrication of patterns of adherent marine bacterium phaeobacter inhibens using soft lithography and scanning probe lithography.	two lithographic approaches have been explored for the microfabrication of cellular patterns based on the attachment of marine bacterium phaeobacter inhibens strain t5. strain t5 produces a new antibiotic that makes this bacterium potentially interesting for the pharmaceutical market and as a probiotic organism in aquacultures and in controlling biofouling. the microcontact printing (microcp) method is based on the micropatterning of self-assembled monolayers (sams) terminated with adhesive end ...	2010	20397716
ability of pseudoalteromonas tunicata to colonize natural biofilms and its effect on microbial community structure.	we investigated the effectiveness of surface colonization by the epiphytic marine bacterium pseudoalteromonas tunicata firstly on a complex biofilm community on glass slides, and secondly, on the epiphytic community of ulva australis. the effectiveness of p. tunicata was compared with the performance of phaeobacter sp. 2.10, also a marine epiphytic isolate in the u. australis colonization experiments. pseudoalteromonas tunicata cells were able to colonize the glass slide community at densities f ...	2010	20579099
litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach.	a gram-reaction-negative, strictly aerobic, non-motile bacterium, designated strain g4(t), was isolated from a sandy beach of taean in south korea. cells were ovoid rods and were catalase- and oxidase-positive. growth of strain g4(t) was determined at 15-35 °c (optimum 25-30 °c) and ph 6-8 (optimum ph 6.5-7.5). strain g4(t) contained q-10 as the predominant isoprenoid quinone and c(18 : 1)ω7c (59.0 %), c(18 : 1)ω7c 11-methyl (11.3 %) and c(12 : 1) 3-oh (9.8 %) as the major fatty acids. the major ...	2010	20639225
primorskyibacter sedentarius gen. nov., sp. nov., a novel member of the class alphaproteobacteria from the shallow sediments from the sea of japan.	two gram-negative, aerobic, non-pigmented, non-motile rod-shaped bacteria kmm 9015 and kmm 9018 t were isolated from a shallow sediment sample collected from the sea of japan. an analysis of the nearly complete 16s rrna gene sequences showed that the isolates are very close phylogenetically (99.9% sequence similarity) to each other and their close relatives were marinovum algicola (95.8 and 95.9%, respectively) and members of the genera leisingera (95.7-95.1 and 95.8-95.2%), phaeobacter (95.0-94 ...	2010	20675440
origin and evolution of a novel dnaa-like plasmid replication type in rhodobacterales.	large extrachromosomal elements are widespread among alphaproteobacteria, but it is unclear how up to a dozen low-copy plasmids can stably coexist within the same cell. we systematically analyzed the distribution of different replicons in about 40 completely sequenced genomes of the roseobacter clade (rhodobacterales) and surprisingly identified a novel plasmid replicon type. the conserved replication module comprises the characteristic partitioning operon (parab) and a hitherto unknown replicas ...	2010	21097494
effects of three heavy metals on the bacteria growth kinetics: a bivariate model for toxicological assessment.	the effects of three heavy metals (co, ni and cd) on the growth kinetics of five bacterial strains with different characteristics (pseudomonas sp., phaeobacter sp. strain 27-4, listonella anguillarum, carnobacterium piscicola and leuconostoc mesenteroides subsp. lysis) were studied in a batch system. a bivariate model, function of time and dose, is proposed to describe simultaneously all the kinetic profiles obtained by incubating a microorganism at increasing concentrations of individual metals ...	2011	21360150
the jekyll-and-hyde chemistry of phaeobacter gallaeciensis.	emiliania huxleyi, an environmentally important marine microalga, has a bloom-and-bust lifestyle in which massive algal blooms appear and fade. phaeobacter gallaeciensis belongs to the roseobacter clade of a-proteobacteria, the populations of which wax and wane with that of e. huxleyi. roseobacter are thought to promote algal growth by biosynthesizing and secreting antibiotics and growth stimulants (auxins). here we show that p. gallaeciensis switches its secreted small molecule metabolism to th ...	2011	21430694
genome announcement: draft genome of phaeobacter gallaeciensis ang1; a dominant member of the accessory nidamental gland of euprymna scolopes.	phaeobacter gallaeciensis strain ang1 represents the dominant member of the bacterial consortium within the reproductive accessory nidamental gland (ang) of the squid euprymna scolopes. we present a 4.59mb assembly of its genome, which may provide clues as to how it benefits its host.	2011	21551313
tropicibacter multivorans sp. nov., an aerobic alphaproteobacterium isolated from surface seawater.	strain md5t, an aerobic marine alphaproteobacteria, was isolated from mediterranean seawater at the malvarrosa beach of valencia, spain. the strain was characterized following a polyphasic study and situated within the roseobacter clade in the family rhodobacteraceae. the phylogenetic analysis based on 16s rrna gene sequences showed that strain md5t is related to tropicibacter naphthalenivorans co2t, phaeobacter inhibens t5t, p. gallaeciensis bs107t and p. daeponensis tf-218t, with 96.9 %, 96.2 ...	2011	21602360
biological versus technical variability in 2-d dige experiments with environmental bacteria.	two-dimensional difference gel electrophoresis (2-d dige) allows for reliable quantification of global protein abundance changes. the threshold of significance for protein abundance changes depends on the experimental variation (biological and technical). this study estimates biological, technical and total variation inherent to 2-d dige analysis of environmental bacteria, using the model organisms "aromatoleum aromaticum" ebn1 and phaeobacter gallaeciensis dsm 17395. of both bacteria the solubl ...	2011	21751370
roseobacticides: small molecule modulators of an algal-bacterial symbiosis.	marine bacteria and microalgae engage in dynamic symbioses mediated by small molecules. a recent study of phaeobacter gallaeciensis, a member of the large roseobacter clade of α-proteobacteria, and emiliania huxleyi, a prominent member of the microphytoplankton found in large algal blooms, revealed that an algal senescence signal produced by e. huxleyi elicits the production of novel algaecides, the roseobacticides, from the bacterial symbiont. in this report, the generality of these findings ar ...	2011	21928816
Tropodithietic acid production in Phaeobacter gallaeciensis is regulated by N-acyl homoserine lactone-mediated quorum sensing.	The production of N-acyl homoserine lactones (AHLs) is widely distributed within the marine Roseobacter clade, and it was proposed that AHL-mediated quorum sensing (QS) is one of the most common cell-to-cell communication mechanisms in roseobacters. The traits regulated by AHL-mediated QS are yet not known for members of the Roseobacter clade, but production of the antibiotic tropodithietic acid (TDA) was supposed to be controlled by AHL-mediated QS in Phaeobacter spp. We describe here for the f ...	2011	21949069
marine bacteria from danish coastal waters show antifouling activity against the marine fouling bacterium pseudoalteromonas sp. strain s91 and zoospores of the green alga ulva australis independent of bacteriocidal activity.	the aims of this study were to determine if marine bacteria from danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. we further assess if antibacterial effect is a good proxy for antifouling activity. we isolated 110 bacteria with anti-vibrio activity from different sample types and locations during a 1-year sampling from danish coastal waters. the strains were identified as pseudoalteromonas, phaeobacter, and vibrionac ...	2011	22003011
Genomes and Virulence Factors of Novel Bacterial Pathogens Causing Bleaching Disease in the Marine Red Alga Delisea pulchra.	Nautella sp. R11, a member of the marine Roseobacter clade, causes a bleaching disease in the temperate-marine red macroalga, Delisea pulchra. To begin to elucidate the molecular mechanisms underpinning the ability of Nautella sp. R11 to colonize, invade and induce bleaching of D. pulchra, we sequenced and analyzed its genome. The genome encodes several factors such as adhesion mechanisms, systems for the transport of algal metabolites, enzymes that confer resistance to oxidative stress, cytolys ...	2011	22162749