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identification of pban-like peptides in the brain-subesophageal ganglion complex of lepidoptera using western-blotting.an immunoblotting technique used to visualize pheromone-biosynthesis-activating-neuropeptide (pban)-like peptides in insect tissues is described. this technique involves a tricine-sds-page system and a chemiluminescent revelation of the antigens. using this technique, pban-like immunoreactive peptides were found in the brain-subesophageal ganglion complex of various lepidopteran species, including moths: heliothis zea, mamestra brassicae, spodoptera littoralis, s. latifascia and s. descoinsi (no ...19968882661
desymmetrization of 1,4-dien-3-ols and related compounds via ueno-stork radical cyclizations[formula: see text] desymmetrization of 1,4-dien-3-ols and related compounds via ueno-stork radical cyclizations is reported. the stereochemistry of the cyclization is controlled by the acetal center. excellent stereocontrol at c(4) and c(5) of the newly formed tetrahydrofuran rings is observed. use of a chiral auxiliary allows the preparation of enantiomerically pure material. the utility of this method has been demonstrated by achieving a short synthesis of (+)-eldanolide, the pheromone of the ...200010804554
biocontrol of the sugarcane borer eldana saccharina by expression of the bacillus thuringiensis cry1ac7 and serratia marcescens chia genes in sugarcane-associated bacteria.the cry1ac7 gene of bacillus thuringiensis strain 234, showing activity against the sugarcane borer eldana saccharina, was cloned under the control of the tac promoter. the fusion was introduced into the broad-host-range plasmid pkt240 and the integration vector pjff350 and without the tac promoter into the broad-host-range plasmids pml122 and pkmm0. these plasmids were introduced into a pseudomonas fluorescens strain isolated from the phylloplane of sugarcane and the endophytic bacterium herbas ...200010877771
natural enemies of lepidopterous borers on maize and elephant grass in the forest zone of cameroon.the importance, geographical and temporal distributions of parasitoids of lepidopterous borers on maize and elephant grass, pennisetum purpureum, were assessed during surveys in farmers' fields in six villages and two on-station trials in the forest zone of cameroon between 1995 and 1996. the borer species encountered were busseola fusca (fuller), sesamia calamistis hampson, eldana saccharina walker on both host plants, and mussidia nigrivenella ragonot on maize only. busseola fusca was the pred ...200111415475
distribution, relative importance and effect of lepidopterous borers on maize yields in the forest zone and mid-altitude of cameroon.geographic distribution, relative importance, and effect of lepidopterous stem and ear borers on maize, zea mays l., were studied in the humid forest and the mid-altitude zones of cameroon from 1994 to 1997. six villages were chosen in the forest zone and grouped into three blocks representing gradients in human population density. a single block with three villages was chosen in the mid-altitude. farmers' maize fields were surveyed during the vegetative growth phase for busseola fusca (fuller) ...200111777046
multi-trophic level interactions in a cassava-maize mixed cropping system in the humid tropics of west africa.multi-trophic level interactions in a mixed crop, involving cassava and maize, were studied in derived-savanna in benin, west africa. two trials were planted, one during the short rainy season two months before onset of the dry season and one during the long rainy season in spring. key pests under study on maize were the noctuid sesamia calamistis hampson and the pyralids eldana saccharina walker and mussidia nigrivenella ragonot, and on cassava, the exotic mealybug, phenacoccus manihoti matile- ...200415191627
construction of a bioinsecticidal strain of pseudomonas fluorescens active against the sugarcane borer, eldana saccharina.a cryia(c) gene was cloned from a native bacillus thuringiensis strain showing activity against the sugarcane borer, eldana saccharina. the sequence of the cloned gene was very similar to that of the b. thuringiensis subsp. kurstaki hd-73 cryia(c) gene. the gene was introduced into an isolate of pseudomonas fluorescens, capable of colonizing sugarcane, on two broad-host-range plasmids, pder405 and pkt240, having copy numbers of 13 and 28, respectively. by using the omegon-km vector, the cry gene ...199416349194
status of eldana saccharina (lepidoptera: pyralidae), its host plants and natural enemies in ethiopia.surveys for sugarcane stem borers were undertaken in ethiopia to determine the prevalence and distribution of these and their natural enemies in crops and indigenous host plants. eldana saccharina walker was not recovered from sugarcane, but was present in three indigenous wetland sedges, cyperus papyrus, c. fastigiatus and c. dives in the southern, central and northern part of the country. the latter indigenous host plant was present in waterways adjacent to sugarcane on the commercial sugar es ...200617092361
water stress augments silicon-mediated resistance of susceptible sugarcane cultivars to the stalk borer eldana saccharina (lepidoptera: pyralidae).silicon (si) can improve resistance of plants to insect attack and may also enhance tolerance of water stress. this study tested if si-mediated host plant resistance to insect attack was augmented by water stress. four sugarcane cultivars, two resistant (n21, n33) and two susceptible (n26, n11) to eldana saccharina walker were grown in a pot trial in si-deficient river sand, with (si+) and without (si-) calcium silicate. to induce water stress, irrigation to half the trial was reduced after 8.5 ...200717411480
the role of wild grasses in the management of lepidopterous stem-borers on maize in the humid tropics of western africa.sites in the humid forest of cameroon and the derived savanna of benin were selected to evaluate the effect of planting border rows of wild host plants on lepidopterous stem-borer infestations and on maize yield. grass species were chosen that in surveys and greenhouse trials were highly attractive to ovipositing female moths but with offspring mortality of close to 100%, thus acting as trap plants. in cameroon, elephant grass pennisetum purpureum moench significantly lowered infestations of bus ...200217598302
the effect of endophytic fusarium verticillioides on infestation of two maize varieties by lepidopterous stemborers and coleopteran grain feeders.abstract a series of experiments were conducted to test the effect of the presence of fusarium verticillioides in the maize plant on subsequent infestation by coleopteran and lepidopteran pests. the effect of percent internodes 1 to 5 infected with f. verticillioides, time after planting, and maize variety on attacks of stem and ears by lepidopterous and coleopteran pests was assessed in field experiments in early and late season 1998 and early season 1999 in benin republic. artificial inoculati ...200218943084
within-field damage and distribution patterns of the stalk borer, eldana saccharina (lepidoptera: pyralidae), in sugarcane and a comparison with nematode damage.the occurrence of eldana saccharina (lepidoptera: pyralidae) was monitored in grids represented by plots in 12 nematicide trials in south african sugarcane fields. the trials encompassed a total of eight plant cane crops and 22 ratoon crops and were situated within commercial cane fields. several measurements were made to characterize the damage caused by e. saccharina. these included the number of internodes per stalk, the percentage of internodes damaged and the percentage of stalks damaged. t ...201019860988
population genetics of eldana saccharina walker (lepidoptera: pyralidae) and the implications for management using biocontrol.the african sugarcane stalk borer, eldona saccharina walker (lepidoptera: pyralidae), is widely distributed throughout sub-saharan africa and is an important insect pest of maize and sugarcane. the insect shows significant variation in behaviour, host plant and natural enemy guild in different regions. several attempts to redistribute the natural enemies of e. saccharina from west africa to south africa were unsuccessful. the significant behavioural, host plant and natural enemy variations as we ...201021539263
Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene codes for 130-kDa toxin protein.Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene from Bacillus thuringiensis var. kurstaki borne on pKT230, shuttle vector, was generated. PCR amplification of Cry1Ac gene present in recombinant G. diazotrophicus yielded a 278-bp DNA product. The nitrogenase assay has revealed that the recombinant G. diazotrophicus in sugarcane stem produced similar levels of nitrogenase compared to wild-type G. diazotrophicus. The presence of 130-kDa protein in apoplastic fluid from sugarcane ...201121934327
in vitro generation of somaclonal variant plants of sugarcane for tolerance to fusarium sacchari.key message : a combination of in vitro culture and mutagenesis using ethyl methanesulfonate (ems) followed by culture filtrate-mediated selection produced variant sugarcane plants tolerant and resistant to fusarium sacchari. eldana saccharina is a destructive pest of the sugarcane crop in south africa. fusarium sacchari png40 (a fungal strain harmful to e. saccharina) has the potential to be an endophytic biological control agent of the stalk borer. however, the fungus causes fusarium stalk rot ...201323090725
constituents of wing gland and abdominal hair pencil secretions of male african sugarcane borer,eldana saccharina walker (lepidoptera: pyralidae).in addition totrans-3,7-dimethyl-6-octen-4-olide (eldanolide), vanillin, and 4-hydroxybenzaldehyde, identified by french workers in the wing gland and abdominal hair pencil secretions of the male african sugarcane borer,eldana saccharina, we have, in an earlier note, reported the presence of several other terpenoid, aromatic, and unbranched-chain compounds such as, (z)-3,7-dimethylocta-2,6-dienoic acid, 6,10,14-trimethyl-2-pentadecanol, 4-hydroxy-3-methoxybenzyl alcohol, 1-octadecane thiol, 16-h ...199324248574
evolved variation in cold tolerance among populations of eldana saccharina (lepidoptera: pyralidae) in south africa.among-population variation in chill-coma onset temperature (ctmin ) is thought to reflect natural selection for local microclimatic conditions. however, few studies have investigated the evolutionary importance of cold tolerance limits in natural populations. here, using a common-environment approach, we show pronounced variation in ctmin (± 4 °c) across the geographic range of a nonoverwintering crop pest, eldana saccharina. the outcomes of this study provide two notable results in the context ...201424773121
silicon reduces impact of plant nitrogen in promoting stalk borer (eldana saccharina) but not sugarcane thrips (fulmekiola serrata) infestations in sugarcane.the stalk borer eldana saccharina walker (lepidoptera: pyralidae) is a major limiting factor in south african sugarcane production, while yield is also reduced by sugarcane thrips fulmekiola serrata kobus (thysanoptera: thripidae). borer management options include appropriate nitrogen (n) and enhanced silicon (si) nutrition; the effect of n on sugarcane thrips is unknown. we tested the effects of these nutrients, in combination with resistant (n33) and susceptible (n27) sugarcane cultivars, on e ...201424999349
direct and indirect effects of development temperature on adult water balance traits of eldana saccharina (lepidoptera: pyralidae).for water balance physiology, prior thermal history may pre-condition individuals to be more sparing in their water consumption at a given temperature upon subsequent exposure, or alternatively, may relax constraints on water economy leading to more frivolous use of water at a later stage. here we test these two major alternative hypotheses on the adult life stage of eldana saccharina walker (lepidoptera: pyralidae) by exposing them to different rearing temperatures (acclimation treatments) duri ...201425008194
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