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molecular characterization of two distinct begomoviruses from papaya in china.six papaya samples showing downward leaf curling were collected in guangdong and guangxi provinces, china. the result of tas-elisa showed they were all infected by geminiviruses. comparison of partial dna-a sequences reveals that these virus isolates can be classified into two groups. group i includes isolates g2, g4, g5, g28 and g29 from guangxi province, while isolate gd2 from guangdong province belongs to group ii. the complete dna-a sequence of g2 and gd2 were characterized. sequence compari ...200415550769
[molecular characteristics of dna-a of tomato leaf curl guangdong virus isolate g2].virus isolate g2 was isolated from tomato plants showing leaf curl symptoms in guangdong. the complete nucleotide sequence of g2 dna-a was determined to be 2744 nucleotides encoding six potential orfs, with two (av1 and av2) in virus sense and four (ac1, ac2, ac3 and ac4) in complementary sense. g2 dna-a has a typical characteristics of begomovirus genome organization. blast results showed that g2 dna-a belonged to the begomovirus of geminiviridae. pairwise comparisons of g2 dna-a with those of ...200515847162
siegesbeckia yellow vein virus is a distinct begomovirus associated with a satellite dna molecule.leaf samples of siegesbeckia glabrescens showing yellow vein, enation, and stunting symptoms were collected in guangdong province, china. a specific 500-bp product was consistently detected in total dna extracts, amplified with universal primers specific for members of the genus begomovirus. comparison of partial dna sequences revealed that these virus isolates were identical, and therefore isolates gd13, gd24 and gd27 were selected for further sequence analysis. the complete nucleotide sequence ...200717187292
ageratum yellow vein china virus is a distinct begomovirus species associated with a dnabeta molecule.abstract ageratum conyzoides plants exhibiting yellow vein symptoms, collected near haikou, hainan province, china, contained begomoviral dna-a-like molecules. the complete sequences of the molecules from two samples, hn2 and hn2-19, were shown to consist of 2,768 and 2,748 nucelotides (nt), respectively. these sequences have more than 97% nucleotide sequence identity, but less than 86% identity with other reported begomovirus sequences. in line with the taxonomic convention for begomoviruses, h ...200718943280
molecular characterization and infectivity of papaya leaf curl china virus infecting tomato in china.papaya leaf curl china virus (palcucnv) was previously reported as a distinct begomovirus infecting papaya in southern china. based on molecular diagnostic survey, 13 palcucnv isolates were obtained from tomato plants showing leaf curl symptoms in henan and guangxi provinces of china. complete nucleotide sequences of 5 representative isolates (aj558116, aj558117, aj704604, fn256260, and fn297834) were determined to be 2738-2751 nucleotides, which share 91.7%-97.9% sequence identities with palcuc ...201020104645
comparison of transmission of papaya leaf curl china virus among four cryptic species of the whitefly bemisia tabaci complex.begomoviruses are transmitted by cryptic species of the whitefly bemisia tabaci complex, often in a species-specific manner. papaya leaf curl china virus (palcucnv) has been recorded to infect several crops including papaya, tomato and tobacco in china. to help assess the risks of spread of this virus, we compared the acquisition, retention and transmission of palcucnv among four species of whiteflies, middle east-asia minor 1 (meam1), mediterranean (med), asia 1 and asia ii 7. all four species ...201526486606
molecular identification of tobacco leaf curl disease in sichuan province of china.tobacco leaf curl disease (tlcd) is caused by begomoviruses in geminiviridae, and infected plants exhibit leaf thickening, downward leaf curling, vein swelling as well as stunting symptoms. it is one of the economically important diseases in tropical and subtropical tobacco-growing areas. seven monopartite begomoviruses have been identified causing tlcd in china.201626738931
vector development and vitellogenin determine the transovarial transmission of begomoviruses.the majority of plant viruses are transmitted by insect vectors between hosts, and transovarial transmission of viruses from vector parents to offspring has great significance to their epidemiology. begomoviruses are transmitted by the whitefly bemisia tabaci in a circulative manner and are maintained through a plant-insect-plant cycle. other routes of begomovirus transmission are not clearly known. here, we report that transovarial transmission from female whiteflies to offspring often happens ...201728607073
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