Publications

[localization of associative cyanobacteria on the roots of epiphytic orchids].	this work is the first study of the localization of phototrophic microorganisms in the rhizoplane and velamen of epiphytic orchids, namely on the aerial and substrate roots of acampe papillosa and dendrobium moschatum and on the aerial roots of the phalaenopsis amabilis and dendrobium phalaenopsis. the composition of the bacterial community on the plant roots depended on the conditions of plant growth. under conditions simulating climate of moist tropical forests, the aerial roots proved to be p ...	2003	12698799
[associative cyanobacteria isolated from the roots of epiphytic orchids].	associative cyanobacteria were isolated from the rhizoplane and velamen of the aerial roots of the epiphytic orchids acampe papillosa, phalaenopsis amabilis, and dendrobium moschatum and from the substrate roots of acampe papillosa and dendrobium moschatum. cyanobacteria were isolated on complete and nitrogen-free variants of bg-11 medium. on all media and in all samples, cyanobacteria of the genus nostoc predominated. nostoc, anabaena, and calothrix were isolated from the surface of the a. papi ...	2003	12698800
pacdpk1, a gene encoding calcium-dependent protein kinase from orchid, phalaenopsis amabilis, is induced by cold, wounding, and pathogen challenge.	signaling pathways, specifically calcium and calcium-dependent protein kinase (cdpk), have been implicated in the regulation of stress and developmental signals in plants. here, we reported the isolation and characterization of an orchid, phalaenopsis amabilis, cdpk gene, pacdpk1, by using the rapid amplification of cdna ends (race)-pcr technique. the full length cdna of 2,310 bp contained an open reading frame for pacdpk1 consisting of 593 amino acid residues. sequence alignment indicated that ...	2007	17593367
characterization of the early response of the orchid, phalaenopsis amabilis, to erwinia chrysanthemi infection using expression profiling.	erwinia chrysanthemi is a devastating bacterial pathogen in phalaenopsis amabilis and causes soft-rotting disease by secretion of cell wall-degrading enzymes. however, the molecular mechanisms underlying the interaction of p. amabilis with e. chrysanthemi remain elusive. in this study, early molecular events of the plant in response to the pathogen attack were investigated. the alteration in reactive oxygen species accumulation and peroxidase activity occurred at the site of infection. subsequen ...	2012	22268629
screening a cdna library for protein-protein interactions directly in planta.	screening cdna libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. however, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. we used bimolecular fluorescence complementation technology to screen a plant cdna library against a bait protein directly in plants. as proof of concept, we used the n-terminal fragment of yellow fluorescent protein- or nvenus-ta ...	2012	22623495