Publications

determination of the complete nucleotide sequences of rna1 and rna2 from greasy grouper (epinephelus tauvina) nervous necrosis virus, singapore strain.	the complete nucleotide sequences of rna1 and rna2 from greasy grouper (epinephelus tauvina) nervous necrosis virus (ggnnv), singapore strain, were determined. 5' race and rna ligation were used to obtain the complete nucleotide sequences of the 5' and 3' non-coding regions (ncrs). ggnnv rna1 was determined to be 3103 nt long, containing an orf of 982 aa, while ggnnv rna2 was determined to be 1433 nt long, containing an orf of 338 aa. both ggnnv rnas are longer than those of other published beta ...	2001	11172107
characterization of virus-like particles assembled in a recombinant baculovirus system expressing the capsid protein of a fish nodavirus.	betanodaviruses are causative agents of neurological disorders in several species of fish. we cloned and sequenced the rna2 segment of two grouper viruses isolated from epinephelus malabaricus (malabaricus grouper nervous necrosis virus, mgnnv) and epinephelus lanceolatus (dragon grouper nervous necrosis virus, dgnnv). the sequences of the two rnas were 99% identical and comparison with previously sequenced rna2 segments of fish nodaviruses striped jack nervous necrosis virus, atlantic halibut v ...	2001	11883005
membrane association of greasy grouper nervous necrosis virus protein a and characterization of its mitochondrial localization targeting signal.	localization of rna replication to intracellular membranes is a universal feature of positive-strand rna viruses. the betanodavirus greasy grouper (epinephelus tauvina) nervous necrosis virus (ggnnv) is a positive-rna virus with one of the smallest genomes among rna viruses replicating in fish cells. to understand the localization of ggnnv replication complexes, we generated polyclonal antisera against protein a, the ggnnv rna-dependent rna polymerase. protein a was detected at 5 h postinfection ...	2004	15163743
development and characterization of a new tropical marine fish cell line from grouper, epinephelus coioides susceptible to iridovirus and nodavirus.	the development and characterization of a new tropical marine fish cell line (gs), derived from the spleen of orange spotted grouper, epinephelus coioides is described. the gs cells grow well in leibovitz's l-15 medium supplemented with 10% foetal bovine serum, and have been subcultured more than 200 times. the optimal growth temperature was 27 degrees c. the gs cell culture consisted of mostly fibroblastic cells. the modal diploid chromosome number was 48. gs cell cultures showed advanced cytop ...	2006	16137774
betanodavirus b2 is an rna interference antagonist that facilitates intracellular viral rna accumulation.	betanodaviruses are small positive-sense bipartite rna viruses that infect a wide variety of fish species and are notorious for causing lethal outbreaks in juvenile fish hatcheries worldwide. the function of a small nonstructural protein, b2, encoded by the subgenomic rna3 of betanodaviruses, has remained obscure. greasy grouper nervous necrosis virus, a betanodavirus model, was used to develop a facile dna-based reverse genetics system that recapitulated the virus infection cycle, and we used t ...	2006	16352533
detection of betanodavirus in juvenile barramundi, lates calcarifer (bloch), by antigen capture elisa.	betanodavirus infection of fish has been responsible for mass mortalities in aquaculture hatcheries worldwide. betanodaviruses possess a bipartite single-stranded rna genome consisting of the 3.1 kb rna1 encoding an rna-dependent rna polymerase and the b2 protein, while the 1.4 kb rna2 encodes the viral nucleocapsid protein, alpha. a panel of six monoclonal antibodies against the alpha protein of greasy grouper nervous necrosis virus (ggnnv) was developed for use in diagnostics. all antibodies r ...	2006	16866926
rna-dependent rna polymerase from atlantic halibut nodavirus contains two signals for localization to the mitochondria.	nodaviruses encode an rna-dependent rna polymerase called protein a that is responsible for replication of the viral rna segments. the intracellular localization of protein a from a betanodavirus isolated from atlantic halibut (ahnv) was studied in infected fish cells and in transfected mammalian cells expressing myc-tagged wild type protein a and mutants. in infected cells protein a localized to cytoplasmic structures resembling mitochondria and in transfected mammalian cells the ahnv protein a ...	2007	17602779