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a naturally occurring recombinant dna-a of a typical bipartite begomovirus does not require the cognate dna-b to infect nicotiana benthamiana systemically.species of the genus begomovirus (family geminiviridae) found in the western hemisphere typically have a bipartite genome that consists of two 2.6 kb dna genomic components, dna-a and dna-b. we have identified and cloned genomic components of a new tomato-infecting begomovirus from brazil, for which the name tomato crinkle leaf yellows virus (tcrlyv) is proposed, and a dna-a variant of tomato chlorotic mottle virus (tocmv-[mg-bt1]). sequence analysis revealed that tcrlyv was most closely related ...200312604824
synergism and negative interference during co-infection of tomato and nicotiana benthamiana with two bipartite begomoviruses.in brazil, at least eight begomoviruses including tomato rugose mosaic virus (tormv) and tomato yellow spot virus (toysv) infect tomatoes. toysv symptoms in tomato and nicotiana benthamiana appear earlier and are more severe compared to those of tormv. we investigated the role of several factors in this differential adaptation. to analyze infection kinetics, a single leaf was inoculated and subsequently detached after different periods of time. viral dna accumulation was quantified in plants, vi ...200919282016
recombination and pseudorecombination driving the evolution of the begomoviruses tomato severe rugose virus (tosrv) and tomato rugose mosaic virus (tormv): two recombinant dna-a components sharing the same dna-b.begomoviruses are dicot-infecting, whitefly-transmitted viruses with a genome comprised of one or two molecules of circular, single-stranded dna. in brazil, tomato-infecting begomoviruses have emerged as serious pathogens since the introduction of a new biotype of the insect vector in the mid-1990's. tomato rugose mosaic virus (tormv) and tomato severe rugose virus (tosrv) are often found in tomato fields. the complete sequence of the dna-b components of tosrv and tormv show an identity of 98.2% ...201424708727
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