Publications

molecular diagnosis of human adenoviruses d and e by a phylogeny-based classification method using a partial hexon sequence.	human adenoviruses (hadvs) are the major causes of a variety of acute illnesses. virus isolation and neutralization tests are usually done to identify the causative virus, but these tests are labor-intensive and time-consuming, and standardized antisera are in limited supply. this study investigated a rapid and reliable method of virus identification based on pcr and phylogenetic analysis. the phylogenetic tree constructed by neighbor joining on the basis of the newly determined partial hexon se ...	2004	15071007
enriching the viral-host interactomes with interactions mediated by sh3 domains.	protein-protein interactions play an essential role in the regulation of most cellular processes. the process of viral infection is no exception and many viral pathogenic strategies involve targeting and perturbing host-protein interactions. the characterization of the host protein subnetworks disturbed by invading viruses is a major goal of viral research and may contribute to reveal fundamental biological mechanisms and to identify new therapeutic strategies. to assist in this approach, we hav ...	2010	19882298
isolation of a novel monkey adenovirus reveals a new phylogenetic clade in the evolutionary history of simian adenoviruses.	adenoviruses of primates include human (hadv) and simian (sadv) isolates classified into 8 species (human adenovirus a to g, and simian adenovirus a). in this study, a novel adenovirus was isolated from a colony of cynomolgus macaques (macaca fascicularis) and subcultured in vero cells. its complete genome was purified and a region encompassing the hexon gene, the protease gene, the dna binding protein (dbp) and the 100 kda protein was amplified by pcr and sequenced by primer walking. sequence a ...	2011	21414228
novel adenoviruses in wild primates: high genetic diversity and evidence of zoonotic transmissions.	adenoviruses (advs) broadly infect vertebrate hosts including a variety of nonhuman primates (nhps). in the present study we identified advs in nhps living in their natural habitat, and, through the combination of phylogenetic analyses with information on the habitat and epidemiological settings, we detected possible horizontal transmission events between nhps and humans. wild nhps were analysed with a pan-primate adv-pcr using a degenerate nested primer set that targets the highly conserved ade ...	2011	21835802
using the e4orf6-based e3 ubiquitin ligase as a tool to analyze the evolution of adenoviruses.	e4orf6 proteins from all human adenoviruses form cullin-based ubiquitin ligase complexes that, in association with e1b55k, target cellular proteins for degradation. while most are assembled with cul5, a few utilize cul2. bc-box motifs enable all these e4orf6 proteins to assemble ligase complexes with elongins b and c. we also identified a cul2-box motif used for cul2 selection in all cul2-based complexes. with this information, we set out to determine if other adenoviruses also possess the abili ...	2016	27252531
selection pressure in the human adenovirus fiber knob drives cell specificity in epidemic keratoconjunctivitis.	human adenoviruses (hadvs) contain seven species (hadv-a to -g), each associated with specific disease conditions. among these, hadv-d includes those viruses associated with epidemic keratoconjunctivitis (ekc), a severe ocular surface infection. the reasons for corneal tropism for some but not all hadv-ds are not known. the fiber protein is a major capsid protein; its c-terminal "knob" mediates binding with host cell receptors to facilitate subsequent viral entry. in a comprehensive phylogenetic ...	2016	27512073
in silico structure analysis and epitope prediction of e3 cr1-beta protein of human adenovirus e for vaccine design.	human adenoviruses are divided into 7 species of human adenovirus a to g based on dna genome homology. the human adenovirus e (hadvs-e) genome is a linear, double-stranded dna containing 38 protein-coding genes. wild-type adenoviruses type e, are linked to a number of slight illnesses. the most important part of hadvs-e is e3 cr1-beta protein which controls the host immune response and viral attachment.	2016	28043417