Publications

phylogeny of microorganisms populating a thick, subaerial, predominantly lithotrophic biofilm at an extreme acid mine drainage site.	an unusually thick ( approximately 1 cm) slime developed on a slump of finely disseminated pyrite ore within an extreme acid mine drainage site at iron mountain, near redding, calif. the slime was studied over the period of 1 year. the subaerial form of the slime distinguished it from more typical submerged streamers. phylogenetic analysis of 16s rrna genes revealed a diversity of sequences that were mostly novel. nearest relatives to the majority of sequences came from iron-oxidizing acidophile ...	2000	10966399
recovery of novel bacterial diversity from a forested wetland impacted by reject coal.	sulphide mineral mining together with improperly contained sulphur-rich coal represents a significant environmental problem caused by leaching of toxic material. the savannah river site's d-area harbours a 22-year-old exposed reject coal pile (rcp) from which acidic, metal rich, saline runoff has impacted an adjacent forested wetland. in order to assess the bacterial community composition of this region, composite sediment samples were collected at three points along a contamination gradient (hi ...	2002	12460285
microbial ecology of an extreme acidic environment, the tinto river.	the tinto river (huelva, southwestern spain) is an extreme environment with a rather constant acidic ph along the entire river and a high concentration of heavy metals. the extreme conditions of the tinto ecosystem are generated by the metabolic activity of chemolithotrophic microorganisms thriving in the rich complex sulfides of the iberian pyrite belt. molecular ecology techniques were used to analyze the diversity of this microbial community. the community's composition was studied by denatur ...	2003	12902280
microbial indicator groups in acidic mining lakes.	the salt composition of 14 acidic lakes was analysed, and their microbial communities were compared by their 16s rdna-based single-strand conformation polymorphism (sscp) fingerprints. the lakes were grouped into three chemically distinct types, and operational taxonomic units (otus) were identified in the 16s rdna sscp fingerprints. discriminant analysis between these groups using a multivariate approach showed that not a single organism, but rather a set of seven otus (ferromicrobium acidophil ...	2005	15643943
comparison of ferric iron generation by different species of acidophilic bacteria immobilized in packed-bed reactors.	flooded packed-bed bioreactors, prepared by immobilizing four different species of acidophilic iron-oxidizing bacteria on porous glass beads, were compared for their ferric iron-generating capacities when operated in batch and continuous flow modes over a period of up to 9 months, using a ferrous iron-rich synthetic liquor and acid mine drainage (amd) water. the bacteria used were strains of acidithiobacillus ferrooxidans, leptospirillum ferrooxidans, a ferrimicrobium-like isolate (tstr) and a n ...	2008	17983721
dissimilatory ferrous iron oxidation at a low ph: a novel trait identified in the bacterial subclass rubrobacteridae.	a novel iron-oxidizing acidophilic actinobacterium was isolated from spoil material at an abandoned copper mine. phylogenetic analysis placed the isolate within the rubrobacteridae subclass of the actinobacteria. its optimum temperature and ph for growth are 30-35 degrees c and ph 3.0, respectively. although it could catalyze the dissimilatory oxidation of ferrous iron, growth yields declined progressively in media containing ferrous iron concentrations >100 microm. the isolate, pa33, did not gr ...	2008	18803673
ferrimicrobium acidiphilum gen. nov., sp. nov. and ferrithrix thermotolerans gen. nov., sp. nov.: heterotrophic, iron-oxidizing, extremely acidophilic actinobacteria.	two novel extremely acidophilic, iron-oxidizing actinobacteria were isolated, one from a mine site in north wales, uk (isolate t23(t)), and the other from a geothermal site in yellowstone national park, wyoming, usa (y005(t)). these new actinobacteria belong to the subclass acidimicrobidae, and in contrast to the only other classified member of the subclass (acidimicrobium ferrooxidans), both isolates were obligate heterotrophs. the mine site isolate was mesophilic and grew as small rods, while ...	2009	19406797