Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cryptosporidium andersoni n. sp. (apicomplexa: cryptosporiidae) from cattle, bos taurus. | a new species of cryptosporidium is described from the feces of domestic cattle, bos taurus. oocysts are structurally similar to those of cryptosporidium muris described from mice but are larger than those of cryptosporidium parvum. oocysts of the new species are ellipsoidal, lack sporocysts, and measure 7.4 x 5.5 microm (range, 6.0-8.1 by 5.0-6.5 microm). the length to width ratio is 1.35 (range, 1.07-1.50). the colorless oocyst wall is < 1 microm thick, lacks a micropyle, and possesses a longi ... | 2000 | 10651302 |
| prevalence of giardia sp. cryptosporidium parvum and cryptosporidium andersoni (syn. c. muris) [correction of cryptosporidium parvum and cryptosporidium muris (c. andersoni)] in 109 dairy herds in five counties of southeastern new york. | a cross-sectional study was undertaken to determine the prevalence of giardia sp. (g. duodenalis group), cryptosporidium parvum and cryptosporidium andersoni (c. muris) [corrected] in dairy cattle in three different age groups, and to evaluate the association of age and season with prevalence. one hundred and nine dairy farms, from a total of 212 farms, in five counties of southeastern new york volunteered to participate. on these farms, 2943 fecal samples were collected from three defined age g ... | 2000 | 11027856 |
| prevalence of cryptosporidium, giardia and eimeria infections in post-weaned and adult cattle on three maryland farms. | the prevalence of cryptosporidium, giardia and eimeria, in healthy, asymptomatic, post-weaned and mature cattle was investigated on three maryland farms. one farm, a dairy research facility, had 150 multiparous holstein milking cows; 24 were examined and cryptosporidium andersoni was detected in three (12.5%) but neither giardia nor eimeria was detected. the second farm, a commercial dairy, had 57 multiparous holstein milking cows and an equal number of heifers. of 19 cows examined, c. parvum, g ... | 2000 | 11035228 |
| morphologic, host specificity, and genetic characterization of a european cryptosporidium andersoni isolate. | this study was undertaken in order to characterize a cryptosporidium muris-like parasite isolated from cattle in hungary and to compare this strain with other cryptosporidium species. to date, the large-type oocysts isolated from cattle were considered as c. muris described from several mammals. the size, form, and structure of the oocysts of the hungarian strain were identical with those described by others from cattle. an apparent difference between the morphometric data of c. muris-like paras ... | 2000 | 11191899 |
| cryptosporidium andersoni from a danish cattle herd: identification and preliminary characterisation. | in november 1997, cryptosporidium andersoni, for the first time, was isolated from a danish heifer. the isolate was characterised morphologically, molecularly, and furthermore inoculated into mice and one calf. data on the distribution of cryptosporidia in the herd of origin were obtained at two separate visits in december 1997 and april 1998. c. andersoni was detected in 27 (19.0%) of 142 cattle examined at the first visit, whereas c. parvum was found in six (4.2%). at the following visit 42 (2 ... | 2002 | 12072212 |
| successful in vitro cultivation of cryptosporidium andersoni: evidence for the existence of novel extracellular stages in the life cycle and implications for the classification of cryptosporidium. | the present study describes the complete development of all life cycle stages of cryptosporidium andersoni in the hct-8 cell line. the in vitro cultivation protocols were the same as those used for the successful growth of all life cycle stages of cryptosporidium parvum (int. j. parasitol. 31 (2001) 1048). under these culture conditions, c. andersoni grew and proliferated rapidly with the completion of the entire life cycle within 72h post-infection. the developmental stages of c. andersoni are ... | 2002 | 12464418 |
| characteristics of a novel type of bovine cryptosporidium andersoni. | we isolated oocysts that resemble cryptosporidium andersoni from cattle grazing on a farm in japan. the partial sequences of genes from the isolate were coincident with published sequences of genes of c. andersoni. since the isolate was able to infect scid mice, the isolate appears to be a novel type of c. andersoni. | 2003 | 12514063 |
| prevalence and infection pattern of naturally acquired giardiasis and cryptosporidiosis in range beef calves and their dams. | the prevalence and infection pattern of naturally acquired giardiasis and cryptosporidiosis in 20 ranch raised beef calves and their dams from birth to weaning was determined. rectal fecal samples were collected from calves at 3 days of age and weekly thereafter; cows' fecal samples were collected at the time of calving, 1 week later and four times during the summer grazing period. blood samples were collected from the calves at 3 days of age to determine igg(1) concentrations. giardia lamblia c ... | 2003 | 12781473 |
| genetic diversity of cryptosporidium spp. in cattle in michigan: implications for understanding the transmission dynamics. | epidemiological and molecular data on 248 bovine, 17 human, and 16 water samples of cryptosporidium spp. collected from the lower peninsula of michigan between 1997 and 2000 were analysed. cryptosporidium parvum bovine genotype and cryptosporidium andersoni were found in 56 and four cattle samples, respectively. a total of six c. parvum subgenotypes were found in 34 bovine samples, and five of the eight farms had two or three subgenotypes in cattle. six water samples from these farms had c. ande ... | 2003 | 12783304 |
| infection of human and bovine epithelial cells with cryptosporidium andersoni induces apoptosis and disrupts tight junctional zo-1: effects of epidermal growth factor. | the effects of cryptosporidium andersoni on human or bovine epithelia are poorly defined. epidermal growth factor inhibits colonisation of the gastrointestinal epithelium with bacteria and the enteric protozoan parasite giardia lamblia. this study characterised whether c. andersoni infects human or bovine epithelial cells in vitro, assessed its impact on apoptosis and tight junctional zonula-occludens-1, and determined whether these effects may be altered by epidermal growth factor. monolayers o ... | 2003 | 14527519 |
| prevalence and pathogenicity of cryptosporidium andersoni in one herd of beef cattle. | over a 35-week period from january to july 2002, a breed of hereford beef cattle (h) and their hybrids were monitored. five hundred and ninety-nine individual fecal samples from calves and 96 samples from their mothers were examined. first excretion of cryptosporidium andersoni oocysts in calves was found in the 9th week after the start of calving (a calf 63-day old). the prevalence of c. andersoni in calves ranged from 11.1 to 92.9% depending on age. the mean prevalence in their mothers was fou ... | 2003 | 14633200 |
| comparison of selected diagnostic methods for identification of cryptosporidium parvum and cryptosporidium andersoni in routine examination of faeces. | this study involved the comparison of suitability of different methods for routine diagnostics of cryptosporidium spp. two staining methods, one concentration-sedimentation method, seven concentration-floatation methods and one combined floatation-sedimentation method were compared. the methods were tested with two concentrations (1 x 10(5) and 1 x 10(6)/g) of c. parvum and c. andersoni. the methods were evaluated using light microscope, magnification 400x for concentration methods and 1000x for ... | 2003 | 14633212 |
| prevalence and age-related variation of cryptosporidium species and genotypes in dairy calves. | fifteen dairy farms in seven states on the east coast of the us were each visited on two consecutive years to determinate the prevalence of cryptosporidium species in pre-weaned (5 days to 2 months) and post-weaned calves (3-11 months), respectively. after each of 971 fecal specimens collected directly from each calf was sieved and subjected to density gradient centrifugation to remove debris and concentrate oocysts, specimens were examined by immunofluorescence microscopy, and polymerase chain ... | 2004 | 15177715 |
| comparison of digestive microflora between feedlot cattle with and without infection by cryptosporidium andersoni. | the influence of cryptosporidial abomasitis on digestive anaerobic microflora in feedlot cattle with spontaneous cryptosporidium andersoni colonization of abomasum was shown. significant differences were found after the cultivation of abomasal content. scopulariopsis brevicaulis was detected in damaged areas of infected abomasum. pcr analysis of ruminal fluid showed no differences between the two animal groups regarding qualitative composition of anaerobic and facultative anaerobic rumen microfl ... | 2004 | 15227789 |
| the detection of a novel type of cryptosporidium andersoni oocyst in cattle in japan. | cryptosporidium muris, found in rodents and cattle, has been recognized as a valid species. however, this organism from cattle was recently separated from c. muris infecting rodents based on molecular data and a transmission study. as a consequence, it has been proposed as a new species, c. andersoni. more recently, c. andersoni, which has infectivity to rodents, was detected in cattle in japan, where it has been designated as a novel type. however, isolates from cattle in japan have not been an ... | 2004 | 15232735 |
| failed attempt of cryptosporidium andersoni infection in lambs. | four lambs 4 months old were inoculated with cryptosporidium andersoni oocysts (bovine isolate) in a dose of 5 x 10(6) per animal followed later by 1 x 10(7) oocysts per animal. no animal shed cryptosporidium oocysts during 56 days post infection, no lamb showed clinical symptoms of cryptosporidiosis and no macroscopic changes were detected in the abomasum immediately after autopsy. histological examinations did not demonstrate the occurrence of cryptosporidium in the abomasum and other selected ... | 2004 | 15729951 |
| prevalence and characterisation of cryptosporidium species in cattle faeces and on beef carcases at slaughter. | cattle are known reservoirs and asymptomatic excretors of cryptosporidium, a protozoan parasite that causes severe and protracted diarrhoea in people. the incidence of cryptosporidium was investigated in 288 matched samples taken from beef carcases of 1 g samples of faeces retrieved immediately after de-legging, 25 cm2 samples of beef excised from the rump of uneviscerated carcases, and 25 cm2 samples of beef excised from the brisket area of eviscerated carcases. cryptosporidium species were det ... | 2005 | 15736696 |
| detection of cryptosporidium andersoni in a dairy cow with diarrhoea in the uk. | 2005 | 15816199 | |
| infectivity of a novel type of cryptosporidium andersoni to laboratory mice. | previously, we reported 'a novel type' of cryptosporidium andersoni detected from cattle in japan, and showed that the isolate was infective to mice. in the present study, we examined the patterns of oocyst shedding in both immunocompromised and immunocompetent mice, as well as pathological lesions in the infected mice. after oral inoculation with 1 x 10(6) oocysts, all five severe combined immunodeficiency (scid) mice began to shed endogenously produced oocysts on day 6 post-inoculation (p.i.). ... | 2005 | 15817217 |
| isolation of cryptosporidium andersoni kawatabi type in a slaughterhouse in the northern island of japan. | fecal samples were collected from 325 adult cattle and 108 pigs in a slaughterhouse in hokkaido, the northern island of japan. five adult cattle were found to be positive for oocysts of cryptopsoridium (1.5%). the oocysts were morphologically similar to those of cryptosporidium andersoni. the partial sequence of the 18s rrna gene of the isolate was 100% identical with that of the c. andersoni kawatabi strain. scid mice were infected after oral administration. based on the morphology of the oocys ... | 2005 | 15878239 |
| new genotypes and factors associated with cryptosporidium detection in mussels (mytilus spp.) along the california coast. | a 3 year study was conducted to evaluate mussels as bioindicators of faecal contamination in coastal ecosystems of california. haemolymph samples from 4680 mussels (mytilus spp.) were tested for cryptosporidium genotypes using pcr amplification and dna sequence analysis. our hypotheses were that mussels collected from sites near livestock runoff or human sewage outflow would be more likely to contain the faecal pathogen cryptosporidium than mussels collected distant to these sites, and that the ... | 2005 | 15993883 |
| prevalence of species and genotypes of cryptosporidium found in 1-2-year-old dairy cattle in the eastern united states. | the prevalence of cryptosporidium species in 1-2-year-old heifers was determined for 571 animals on 14 dairy farms in seven states on the east coast of the united states. a fecal specimen collected directly from each heifer was processed to concentrate oocysts that were then examined by polymerase chain reaction (pcr). for every pcr-positive specimen the 18s rrna gene of cryptosporidium was sequenced. cryptosporidium was identified by pcr from heifers on 13 of 14 farms. on all except four farms ... | 2006 | 16159697 |
| morphological and immunohistochemical features of cryptosporidium andersoni in cattle. | light and electron microscopic features and immunohistochemical features of cryptosporidium andersoni (c. andersoni) and host reaction in the mucosa were studied. although the affected cattle demonstrated no apparent clinical signs, a severe infection of c. andersoni was observed in the abomasum. c. andersoni were round in shape, measured 6-8 microm in size and were mainly observed to be freely located in the gastric pits, being attached in occasional cases to the surface of the abomasum epithel ... | 2006 | 16537941 |
| genetic analysis of cryptosporidium from 2414 humans with diarrhoea in england between 1985 and 2000. | the characterization of cryptosporidium using dna extracted from whole faecal samples collected from 2414 humans with diarrhoea in england between 1985 and 2000 where cryptosporidial oocysts were detected using conventional methods is described. characterization was achieved by pcr/rflp and dna sequencing of fragments of the cryptosporidium oocyst wall protein and the 18s rdna genes. cryptosporidium parvum was detected in 56.1 % of cases, cryptosporidium hominis in 41.7 % and a mixture of c. par ... | 2006 | 16687587 |
| sample prevalence and molecular characterisation of cryptosporidium andersoni within a dairy herd in the united kingdom. | bovine cryptosporidiosis is usually an acute diarrhoeal disease of young calves caused by cryptosporidium parvum. however, chronic infection with cryptosporidium andersoni has been associated with gastritis, reduced milk yield and poor weight gain in adult cattle. here we describe the first genetic confirmation and characterisation of c. andersoni from cattle in the united kingdom and its sample prevalence within a dairy herd. oocysts measured 7.5+/-0.4 microm x 5.5+/-0.4 microm (7.0-8.5 microm ... | 2006 | 16908101 |
| infectivity and pathogenicity of cryptosporidium andersoni to a novel host, southern multimammate mouse (mastomys coucha). | the infectivity and pathogenicity of cryptosporidium andersoni (bovine isolate) for neonatal and adult southern multimammate mice (mastomys coucha) was studied using transmission experiments. c. andersoni isolate used in this study was not infective for balb/c mice, but experimental infection proved susceptibility of neonatal and adult m. coucha to the infection. the prepatent period was 20-24 days, the patent period varied between 46 and 59 days. no signs of clinical illness or macroscopic find ... | 2007 | 16997473 |
| viability staining and animal infectivity of cryptosporidium andersoni oocysts after long-term storage. | preservation of biological properties of oocysts during the storage is critical for experimental work. stomach species of cryptosporidia are less resistant against external influences, and their infectivity decreases rapidly in comparison with intestinal cryptosporidia. cryptosporidium andersoni oocysts lost their infectivity for gerbil (meriones unguiculatus) after 7 months storage in deionised water (dw) or in 2.5% potassium dichromate solution (pds). evaluation of oocyst viability by flow cyt ... | 2007 | 17024363 |
| identification of novel cryptosporidium genotypes from avian hosts. | a total of 430 avian-derived fecal specimens were randomly collected from selected western australian commercial aviaries, poultry farms, hatcheries, wildlife parks, and the perth zoo and screened for the presence of cryptosporidium by pcr. of these, 27 cryptosporidium-positive isolates were detected, characterized, and compared with 11 avian-derived isolates from the czech republic at the 18s rrna and actin gene loci. sequence and phylogenetic analysis identified four genetically distinct genot ... | 2006 | 17028234 |
| giardiasis and cryptosporidiosis in ruminants. | although they differ considerably with respect to their biology, both giardia duodenalis and cryptosporidium parvum are common in ruminants, whereas cryptosporidium andersoni is not. g. duodenalis infections are acquired during the first few months of life, tend to be chronic, and may be a production-limiting disease of ruminants. c. parvum infections remain an important cause of diarrhea in neonatal ruminants. abomasal cryptosporidiosis, caused by c. andersoni, is an emerging disease of cattle ... | 2006 | 17071357 |
| pcr amplification and sequence analyses of its-1 rdna from cryptosporidium andersoni in dairy cattle. | the internal transcribed spacer 1 (its-1) of the ribosomal dna (rdna) from gd, hn, and ah strains of cryptosporidium andersoni was amplified and sequenced to assess whether the its-1 rdna could be used as genetic markers of c. andersoni from different geographic origins in china and to differentiate c. andersoni from other cryptosporidium species. the result showed that the its-1 sequences of gd, hn, and ah strains were basically identical, which were unequivocally different with the sequences o ... | 2007 | 17096139 |
| wide geographic distribution of cryptosporidium bovis and the deer-like genotype in bovines. | recent studies in the united states reported that approximately 85% of pre-weaned dairy calves were infected with zoonotic cryptosporidium parvum, whereas only 1-2% of post-weaned calves and 1-2-year-old heifers were infected with this species. cryptosporidium bovis and cryptosporidium deer-like genotype were much more prevalent in the post-weaned animals. it is not clear whether the same infection pattern also occurs in other geographic areas. in this study, to determine whether the same crypto ... | 2007 | 17097231 |
| prevalence of cryptosporidium species and genotypes in mature dairy cattle on farms in eastern united states compared with younger cattle from the same locations. | feces collected from 541 milking cows on two dairy farms each in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida were examined for the presence of cryptosporidium oocysts. oocysts were concentrated from 15 g of feces from each cow and dna was extracted. a two-step nested pcr protocol was used to amplify an 830 base pair fragment of the ssurrna gene. pcr-positive products were purified and sequenced. pcr-positive findings were obtained from cows in all seven state ... | 2007 | 17287086 |
| tracking host sources of cryptosporidium spp. in raw water for improved health risk assessment. | recent molecular evidence suggests that different species and/or genotypes of cryptosporidium display strong host specificity, altering our perceptions regarding the zoonotic potential of this parasite. molecular forensic profiling of the small-subunit rrna gene from oocysts enumerated on microscope slides by u.s. environmental protection agency method 1623 was used to identify the range and prevalence of cryptosporidium species and genotypes in the south nation watershed in ontario, canada. fou ... | 2007 | 17483276 |
| detection of a mixed infection of a novel cryptosporidium andersoni and its subgenotype in japanese cattle. | cryptosporidium oocysts were detected in the feces of cattle in saga, japan. isolates were morphologically large. we attempted to identify the species or genotypes of the isolates by analyzing the partial sequences of the 18s rrna and cryptosporidium oocyst wall protein (cowp) genes, and measuring the infectivity in mice. the isolates showed 100% homology with cryptosporidium andersoni in the cowp gene sequence and it could be transmitted to mice, but in the 18s rrna gene, there was an additiona ... | 2007 | 17825491 |
| prevalence and first genetic identification of cryptosporidium spp. in cattle in central viet nam. | we investigated the prevalence of cryptosporidium infection in relation to age and clinical status in cattle in the central region of viet nam. a total of 266 fecal samples from diarrheic and non-diarrheic cattle were examined by the modified ziehl-neelsen staining method. prevalence of cryptosporidium parvum type infections, those of the cryptosporidium andersoni type, and mixed infection of both types was 33.5% (89/266), 5.6% (15/266), and 3.4% (9/266), respectively. the infection rate of 44.3 ... | 2007 | 17964078 |
| [cloning, expression and analysis of the heat shock protein of cryptosporidium andersoni]. | to clone and express the partial encoding sequence of mr 70,000 heat shock protein of cryptosporidium andersoni (cahsp70) in escherichia coli and identify the recombinant protein. | 2007 | 18038769 |
| multilocus phylogenetic analysis of cryptosporidium andersoni (apicomplexa) isolated from a bactrian camel (camelus bactrianus) in china. | this is the first report of cryptosporidiosis in a bactrian camel (camelus bactrianus) in china. two cryptosporidium isolates derived from the same bactrian camel (3-year-old) in november 2005 and april 2006 were characterized using sequence and phylogenetic analysis of the small-subunit rrna (18s rrna), 70-kda heat shock protein (hsp70), actin and cryptosporidium oocyst wall protein (cowp) genes. the sequences of the 18s rrna and cowp were identical to all other cryptosporidium andersoni isolat ... | 2008 | 18165930 |
| prevalence and genotyping of cryptosporidium species from farm animals in mongolia. | the presence of cryptosporidium oocysts in 460 animals (439 cattle, 16 kids, and 5 sheep) of tuv-aimak mongolian district was investigated by ift. cryptosporidium oocysts were found in 116 (26.4%) cattle. out of the 116 ift positive samples, 47 were further purified by ims, investigated by pcr and 11 were found positive. the species and/or genotypes were determined by nested pcr-rflp and sequence analysis of a fragment of the ssu rrna gene. the results indicated the presence of cryptosporidium a ... | 2008 | 18259778 |
| infectivity, pathogenicity, and genetic characteristics of mammalian gastric cryptosporidium spp. in domestic ruminants. | farm ruminants were infected experimentally with four mammalian gastric cryptosporidium, namely cryptosporidium andersoni li03 originated from cattle and three isolates of cryptosporidium muris from brown rat (isolate rn66), bactrian camel (isolate cb03) and firstly characterized isolate from east african mole rat (isolate ts03). sequence characterizations of the small-subunit rrna gene showed that the li03 isolate was c. andersoni and the other three isolates belonged to c. muris, although the ... | 2008 | 18343038 |
| detection and species identification of cryptosporidium from taiwan feeding animals. | in this study, 107 fecal specimens were collected from 40 sampling sites in taiwan livestock and avian farms to test for cryptosporidium spp. oocysts. ten of 107 samples analyzed by enzyme-linked immunosorbent assay showed the presence of cryptosporidium spp., among which 6 samples were simultaneously confirmed by immunofluorescence assay and polymerase chain reaction. nucleic acid sequencing of the 18s rrna gene identified 3 clusters of cryptosporidium spp. three cryptosporidium parvum isolates ... | 2008 | 18372647 |
| [multiplex pcr for analysis of the plasmodium falciparum drug resistance molecular markers]. | to develop a multiplex pcr protocol for amplification of five plasmodium falciparum drug resistance related genes, thereby facilitate the rapid and high throughput analysis of the drug resistance molecular markers. | 2007 | 18441889 |
| contribution of treated wastewater to the contamination of recreational river areas with cryptosporidium spp. and giardia duodenalis. | samples of the influent and final effluent from 12 wastewater treatment plants from galicia (nw, spain) were analyzed for the presence of cryptosporidium spp. oocysts and giardia duodenalis cysts. all of the plants discharge effluent to a hydrographic basin in which there are numerous recreational areas and fluvial beaches. the samples (25-50 liters) were collected in spring, summer, autumn and winter of 2007. a total of 96 samples were analyzed using techniques included in the us environmental ... | 2008 | 18538816 |
| new species of cryptosporidium tyzzer, 1907 (apicomplexa) from amphibian host: morphology, biology and phylogeny. | cryptosporidium fragile sp. n. (apicomplexa) is described from black-spined toads, duttaphrynus melanostictus (schneider) (amphibia, anura, bufonidae) from the malay peninsula. the parasitized animals were directly imported from malaysia and harboured c. fragile at the time of arrival. oocysts were subspherical to elliptical with irregular contour in optical section, measuring 6.2 (5.5-7.0) x 5.5 (5.0-6.5) microm. oocyst wall was smooth and colourless in light microscopy. the endogenous developm ... | 2008 | 18666410 |
| genetical survey of novel type of cryptosporidium andersoni in cattle in japan. | previously, we reported that an isolate of novel type of cryptosporidium andersoni detected in cattle in japan contained type a (identical to c. andersoni reported previously) and type b (having a thymine nucleotide insertion unlike the type a) genotypes in the 18s rrna gene. here, we conducted an extensive investigation of cryptosporidium infections in adult cattle in japan from 2004 to 2007. consequently, cryptosporidium sp. were detected in 12 of the 205 cattle examined (5.9%), and partial se ... | 2008 | 18922640 |
| the first detection of cryptosporidium deer-like genotype in cattle in japan. | the general perception is that cattle are major reservoirs for cryptosporidium parvum infections in humans and that c. parvum is a major cause of diarrhea and production loss in cattle. adult cattle may play an important role as cryptic carrier of the infection. cryptosporidium spp. in asymptomatic adult dairy cattle from some farms around osaki area, miyagi prefecture, japan, was examined on a field visit during august, 2007, by polymerase chain reaction techniques for detection, genotyping, an ... | 2009 | 18989700 |
| prevalence and molecular characterization of bovine cryptosporidium in qazvin province, iran. | the aim of this study was to determine the prevalence, variability with host age, and the genotypes of species of cryptosporidium in cattle from 15 dairy farms in qazvin province, iran. fecal samples, collected from 272 cattle during may 2006 to december 2007, were characterized microscopically. oocysts from 51 positive samples were analyzed using pcr assay of 18s ssu rrna, restriction fragment length polymorphism (rflp) and sequencing. we identified 72.6% of the positive samples as cryptosporid ... | 2009 | 19091477 |
| prevalence of cryptosporidium andersoni: a molecular epidemiological survey among cattle in india. | cryptosporidiosis is an important and established cause of calfhood morbidity in bovines. the present communication reports the prevalence of cryptosporidium infection among juvenile and adult cattle (6-24 months old) in india based on examination of faecal samples collected from 350 animals across three different agro-climatic regions of the country and further confirmation by a two-step nested pcr assay targeting 18s ssu rrna gene. a total of 45 samples were positive for cryptosoridium species ... | 2009 | 19185428 |
| infectivity of gastric and intestinal cryptosporidium species in immunocompetent mongolian gerbils (meriones unguiculatus). | we exposed juvenile (7-day old) and adult (8-week old) mongolian gerbils (meriones unguiculatus) to cryptosporidium andersoni or c. muris, which infect the stomach, or to c. parvum, which infects the intestine. both age groups could be successfully infected with each species in primary mono-infection with juvenile animals demonstrating higher peak oocysts per gram of feces and longer patent periods than adults. concurrent exposure to mixed gastric and intestinal cryptosporidia resulted in succes ... | 2009 | 19394145 |
| prevalence and distribution of cryptosporidium spp. in dairy cattle in heilongjiang province, china. | few data are available on the molecular characterization of cryptosporidium spp. in cattle in china. in the present study, a total of 507 fecal specimens from six dairy farms in heilongjiang province were examined for cryptosporidium spp. by light microscopy of concentrates from the formalin-ethyl acetate sedimentation method (for less than 2-month-old calves) or sheather's floatation method (more than 3-month-old dairy cattle). twenty-seven post-weaned calves on five farms were positive for cry ... | 2009 | 19424720 |
| prevalence and associated management factors of cryptosporidium shedding in 50 swedish dairy herds. | cryptosporidium parvum is a protozoan parasite causing diarrhoea in young calves. this cross-sectional study was performed to estimate the prevalence of cryptosporidium infected herds in a sample of swedish dairy herds and to identify potential risk factors associated with shedding of oocysts. fifty dairy herds, selected by stratified random sampling, were included. the herds were visited once during the indoor seasons of 2005-2006 and 2006-2007. faecal samples were collected from 10 calves, 10 ... | 2009 | 19443061 |
| prevalence and molecular characterization of cryptosporidium spp. in dairy cattle in south bohemia, the czech republic. | a total of 995 samples from slaughtered dairy cattle (6 months and older) were collected during two consecutive years (from 2007 to 2008), stained by aniline-carbol-methyl violet and examined microscopically. dna was extracted from cryptosporidium positive samples and from 200 randomly selected microscopically negative samples. nested pcr was performed to amplify the partial ssu rrna and gp60 genes of cryptosporidium that were subsequently digested by sspi, vspi and mboii restriction enzymes to ... | 2009 | 19616383 |
| effect of select medium supplements on in vitro development of cryptosporidium andersoni in hct-8 cells. | we evaluated the effect of fetal calf serum (fcs), glucose, ascorbic acid, calcium pantothenate, folic acid, and insulin on the growth of cryptosporidium andersoni in human colon tumor (hct-8) cells. after being incubated for 48 h, the proliferation of parasites was determined by real-time polymerase chain reaction (pcr) assay, and the development of c. andersoni was observed by transmission electron microscopy (tem). ten percent fcs was the best concentration for c. andersoni culture. glucose, ... | 2009 | 19641939 |
| a multiplex polymerase chain reaction assay to simultaneously distinguish cryptosporidium species of veterinary and public health concern in cattle. | four species of cryptosporidium are routinely found in cattle: cryptosporidium parvum, cryptosporidium bovis, cryptosporidium ryanae, and cryptosporidium andersoni. it is important to determine the species of cryptosporidium in infected cattle because c. parvum is the only serious pathogen for humans as well as cattle. identification of cryptosporidium species and genotypes currently relies on molecular methods such as polymerase chain reaction (pcr) followed by restriction fragment length polym ... | 2009 | 19713046 |
| cervine genotype is the major cryptosporidium genotype in sheep in china. | to identify cryptosporidium species/genotypes in sheep in china and to elucidate the endemic transmission of cryptosporidiosis, a total of 1,701 fecal samples from five farms in four prefectures in henan province (central china) were examined. eighty-two cryptosporidium-positive samples were analyzed by polymerase chain reaction (pcr)-restriction fragment length polymorphism analysis of the small subunit (ssu) rrna gene and pcr analysis of the 60 kda glycoprotein (gp60) gene, and 41 were further ... | 2010 | 19904561 |
| molecular characterisation of cryptosporidium isolates from swedish dairy cattle in relation to age, diarrhoea and region. | cryptosporidium positive samples from 176 preweaned calves, young stock and cows of 48 herds were subjected to molecular characterisation of the 18s rrna gene to determine which species are present in swedish dairy cattle. in addition, samples characterised as cryptosporidium parvum were further analysed at the gp60 gene to investigate distribution and zoonotic potential of subtypes. the 18s rrna gene was successfully sequenced in 110 samples, with cryptosporidium bovis in 83, c. parvum in 15, c ... | 2010 | 20138705 |
| molecular characterization and assessment of zoonotic transmission of cryptosporidium from dairy cattle in west bengal, india. | few studies in the past have examined the genetic diversity and zoonotic potential of cryptosporidium in dairy cattle in india. to assess the importance of these animals as a source of human cryptosporidium infections, fecal samples from 180 calves, heifers and adults and 51 farm workers on two dairy farms in west bengal, india were genotyped by pcr-rflp analysis of the 18s rrna gene of cryptosporidium followed by dna sequencing of the pcr products. phylogenetic analysis was carried out on the d ... | 2010 | 20356678 |
| seasonal variation in the prevalence and molecular epidemiology of cryptosporidium infection in dairy cattle in the new york city watershed. | we conducted cross-sectional studies in the new york city watershed to ensure a valid estimate of the risk associated with cryptosporidium infection in dairy herds. our aims were to obtain species-specific estimates of the prevalence of cryptosporidium in dairy cattle and to investigate seasonal variations in prevalence. we validated our empirical estimates using a bayesian approach. samples were collected on 32 study farms, once in each of 3 different seasons using an age-stratified sampling de ... | 2010 | 20397026 |
| [modified method for purifying cryptosporidium oocysts]. | sheather's sucrose solution diluted with distilled water as an alternative to pbs was used to purify oocysts of three different cryptosporidium spp. the recovery rate of purified cryptosporidium andersoni, c. baileyi and c. suis oocysts was 47.5%, 49.6% and 41.7%, respectively. the viability of the oocysts was 97.9%, 96.7%, and 95.1%, respectively. after 1 h incubation in a 37 degrees c water bath, the excystation rate of the oocysts was 87.9%, 80.0%, and 81.7%, respectively. | 2010 | 20411762 |
| biological and genetic characterization of cryptosporidium spp. and giardia duodenalis isolates from five hydrographical basins in northern portugal. | to understand the situation of water contamination with cryptosporidium spp. and giardia spp. in the northern region of portugal, we have established a long-term program aimed at pinpointing the sources of surface water and environmental contamination, working with the water-supply industry. here, we describe the results obtained with raw water samples collected in rivers of the 5 hydrographical basins. a total of 283 samples were analyzed using the method 1623 epa, usa. genetic characterization ... | 2010 | 20585525 |
| identification of cryptosporidium species and genotypes in scottish raw and drinking waters during a one-year monitoring period. | we analyzed 1,042 cryptosporidium oocyst-positive slides (456 from raw waters and 586 from drinking waters) of which 55.7% contained 1 or 2 oocysts, to determine species/genotypes present in scottish waters. two nested pcr-restriction fragment length polymorphism (rflp) assays targeting different loci (1 and 2) of the hypervariable region of the 18s rrna gene were used for species identification, and 62.4% of samples were amplified with at least one of the pcr assays. more samples (577 slides; 4 ... | 2010 | 20639357 |
| cryptosporidium infection in herds with and without calf diarrhoeal problems. | a case-control study was designed to investigate the role of different cryptosporidium spp. in swedish dairy herds with and without calf diarrhoeal problems. faecal samples were collected from preweaned calves, young stock and cows. cryptosporidium oocysts were detected by sodium chloride flotation and epifluorescence microscopy. molecular diagnostics were used to identify cryptosporidium species. samples containing c. parvum were further analysed to determine subtypes. calf faecal samples were ... | 2010 | 20714750 |
| cryptosporidium andersoni is the predominant species in post-weaned and adult dairy cattle in china. | dairy industry plays an important role in the agricultural economy of china. to estimate the prevalence and public health significance of cryptosporidiosis in post-weaned and adult dairy cattle in china, during four consecutive years (from 2006 to 2009), a total of 1315 fecal samples from 22 dairy cattle farms in ten prefectures in henan province were examined for the presence of cryptosporidium oocysts. the overall prevalence of cryptosporidium was 7.9%, with the highest infection rate (11.3%) ... | 2010 | 20884374 |
| the potential for zoonotic transmission of giardia duodenalis and cryptosporidium spp. from beef and dairy cattle in ontario, canada. | the objective of this study was to compare the occurrence and the genotypes and species of giardia duodenalis and cryptosporidium spp. in beef and dairy cattle from farms in the regional municipality of waterloo, ontario, in an effort to determine the potential for zoonotic transmission from these animals. pooled manure samples were collected from 45 dairy cattle farms and 30 beef cattle farms. the presence of giardia cysts and cryptosporidium oocysts was determined by immunofluorescence microsc ... | 2010 | 20971563 |
| cryptosporidium andersoni in western australian feedlot cattle. | cryptosporidium andersoni has not been previously reported in feedlot beef cattle in western australia. faecal samples were collected from 10 groups of cattle ranging in age from 11 to 36 months in five different feedlots in western australia. the incidence of c. andersoni ranged from 0% to 26%. there were no clinical signs associated with c. andersoni infection, but there was a significant reduction in rate of gain of 0.44 kg in infected animals compared with negative pen mates. cryptosporidium ... | 2010 | 20973292 |
| development of a multilocus sequence tool for typing cryptosporidium muris and cryptosporidium andersoni. | although widely used for the characterization of the transmission of intestinal cryptosporidium spp., genotyping tools are not available for c. muris and c. andersoni, two of the most common gastric cryptosporidium spp. infecting mammals. in this study, we screened the c. muris whole-genome sequencing data for microsatellite and minisatellite sequences. among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by pcr and dna sequencing, 4 were eventually chosen. dna seque ... | 2010 | 20980577 |
| effect of low ph on the morphology and viability of cryptosporidium andersoni sporozoites and histopathology in the stomachs of infected mice. | the genus cryptosporidium includes many common parasites infecting animals and humans, and is a major cause of diarrheal illness worldwide. the biology of gastric cryptosporidium spp., including replication in the stomach, has not been well documented. this study evaluated the viability of cryptosporidium andersoni sporozoites in gastric environments after excystation and examined the endogenous development and histopathological changes in the stomachs of infected mice, using a novel type of c. ... | 2010 | 21055405 |
| molecular characterization of cryptosporidium spp. in pre-weaned dairy calves in the czech republic: absence of c. ryanae and management-associated distribution of c. andersoni, c. bovis and c. parvum subtypes. | a total of 750 faecal samples of dairy calves at up to 2 months of age kept in various housing systems were screened for cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. dna was extracted from cryptosporidium positive samples and from 150 randomly selected microscopically negative samples. nested pcr was performed to amplify the partial ssu rrna gene of cryptosporidium that was subsequently digested by sspi, vspi and mboii restriction enzymes to determine th ... | 2010 | 21168973 |
| occurrence and molecular characterization of cryptosporidium spp. isolated from domestic animals in a rural area surrounding atlantic dry forest fragments in teodoro sampaio municipality, state of são paulo, brazil. | the aim of this study was to assess the occurrence of cryptosporidium in domestic animals in rural properties surrounding rain forest fragments within the municipality of teodoro sampaio, southeastern brazil. conventional sucrose flotation method followed by molecular characterization of the parasites by sequencing pcr products amplified from ssu rrna gene were used. stool samples were collected from domestic animals raised as pets and livestock in all rural properties surrounding three forest f ... | 2010 | 21184703 |
| molecular characterization of cryptosporidium spp. in native breeds of cattle in kaduna state, nigeria. | despite numerous molecular epidemiologic studies of cryptosporidiosis in dairy cattle in industrialized countries, there are very few studies on the diversity and public health significance of cryptosporidium species in native cattle in developing countries. in this study, a polymerase chain reaction (pcr)-restriction fragment length polymorphism (rflp) analysis of the small-subunit (ssu) rrna gene was used to detect and identify cryptosporidium spp. in 194 fecal specimens from 2 to 365 days old ... | 2011 | 21277091 |
| a recombinant dna vaccine encoding c. andersoni oocyst wall protein induces immunity against experimental c. parvum infection. | cryptosporidium andersoni parasited in the abomasum has been demonstrated as a cause of reduction of milk production in dairy cow. in this study, a novel chimeric dna vaccine pvax1-ab was constructed and the efficacy against cryptosporidium parvum was determined. balb/c mice were divided into 3 groups and immunized with dna vaccine expressing the oocyst wall protein, ab protein of c. andersoni, the recombinant plasmid containing the ab gene, respectively. after inoculation of 1 × 10(6) oocysts o ... | 2011 | 21450406 |
| molecular identification and distribution of cryptosporidium and giardia duodenalis in raw urban wastewater in harbin, china. | contamination of the water supply by protozoa often causes outbreaks of cryptosporidiosis and giardiasis. the goals of the present study was to investigate the level of cryptosporidium and giardia duodenalis in wastewater from wastewater treatment plants in harbin, china, and to understand the endemic transmission characteristics of cryptosporidiosis and giardiasis. forty-eight domestic wastewater specimens from the two wastewater treatment plants in harbin city were collected from april 2009 to ... | 2011 | 21461728 |
| occurrence of cryptosporidium andersoni in brazilian cattle. | abstract feces were collected from 68 dairy cattle, 1 to 12 mo of age, on 12 farms in the municipality of campos dos goytacazes, rio de janeiro, brazil, and examined for the presence of cryptosporidium sp. all samples were subjected to molecular analysis by polymerase chain reaction (nested pcr) of the 18s rrna. four positive samples (4.54%) were sequenced and identified as cryptosporidium andersoni. this species represents a risk for brazilian cattle because infection can affect cattle producti ... | 2011 | 21526922 |
| efficacy of ginkgolic acids against cryptosporidium andersoni in cell culture. | cryptosporidium is a worldwide waterborne parasite and the treatment is a severe problem in immunocompromised patients. in this study, we used the in vitro culture system to evaluate the anti-cryptosporidium activity of ginkgolic acids (gas), nitazoxanide (ntz), garlicin (gar), and artemether (art). the growth of cryptosporidium andersoni in hct-8 cells was determined by real-time pcr assay. when exposed to 5.00 μg/ml gas or 10.00 μg/ml ntz for 48 h, the number of c. andersoni in cultures was on ... | 2011 | 21556686 |
| a whole water catchment approach to investigating the origin and distribution of cryptosporidium species. | aims: investigating the distribution and origin of cryptosporidium species in a water catchment affected by destocking and restocking of livestock due to a foot and mouth disease epidemic. methods and results: surface water, livestock and wildlife samples were screened for cryptosporidium and oocysts characterised by sequencing ssu rrna and cowp loci, and fragment analysis of ml1, ml2 and gp60 microsatellite loci. oocyst concentrations in water samples (0 to 20.29 per 10l) were related to rain ... | 2011 | 21649804 |
| real-time pcr assay targeting the actin gene for the detection of cryptosporidium parvum in calf fecal samples. | cryptosporidium parvum infection is very important with respect to public health, owing to foodborne and waterborne outbreaks and gastrointestinal illness in immunocompetent and immunocompromised persons. in cattle, infection with this species manifests either as a subclinical disease or with diarrheal illness, which occurs more often in the presence of other infectious agents than when alone. the aim of this study was to develop a real-time polymerase chain reaction (pcr) assay for the detectio ... | 2011 | 22042503 |
| occurrence of cryptosporidium and giardia on beef farms and water sources within the vicinity of the farms on prince edward island, canada. | the objectives of this study were to determine the prevalence and assemblages of giardia and species of cryptosporidium on beef farms in prince edward island (pei), canada, including the water sources associated with the farms, and to determine risk factors for infection of cattle with these parasites. twenty beef farms were selected based on the presence of surface water<500m from the barn. prevalence was determined by direct immunofluorescence microscopy, while genotyping and species determina ... | 2011 | 22112978 |
| Detection of Cryptosporidium parvum oocysts in calf fecal samples by direct immunofluorescence assay. | The aim of this study was to produce a conjugate containing anti-Cryptosporidium parvum polyclonal antibodies and standardize a Direct Immunofluorescence Assay (DIF) for detecting C. parvum oocysts in fecal samples from calves. In order to obtain anti-C. parvum polyclonal antibodies, two New Zealand rabbits were immunized with a purified solution of C. parvum oocysts and Freund's adjuvant. Purification of the immunoglobulin G (IgG) fraction was performed by means of precipitation in ammonium sul ... | 2011 | 22166379 |
| variability in susceptibility of voles (arvicolinae) to experimental infection with cryptosporidium muris and cryptosporidium andersoni. | the infectivity of cryptosporidium muris and cryptosporidium andersoni in various species of voles was studied using experimental infections. none of the experimental voles inoculated with 1 × 10(5) oocysts of cryptosporidium spp. shed any oocysts during 40 dpi, except brandt's vole (lasiopodomys brandtii), which was susceptible to c. muris infection. experiments confirmed the resistance of voles of the genus microtus sensu stricto to infection with mammalian gastric cryptosporidia, which provid ... | 2012 | 22302477 |
| molecular prevalence of cryptosporidium spp. in dairy calves in southern states of india. | dung samples were collected from dairy calves of south indian states viz., andhra pradesh, karnataka, kerala, tamil nadu and union territory, puducherry and are subjected to nested polymerase chain reaction (pcr) targeting 18s rrna gene for detection of cryptosporidium infection. of the 459 dung samples screened 182 were found positive with a prevalence of 39.65%. highest prevalence of cryptosporidium was observed in puducherry (86.67%) and lowest in kerala (17.65%). genotyping by pcr-restrictio ... | 2012 | 22459107 |
| cryptosporidium species detected in calves and cattle in dagoretti, nairobi, kenya. | a total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in nairobi, kenya. modified ziehl-neelsen staining method and an immunofluorescence assay were used to identify those samples with cryptosporidium oocyst infection. oocysts from positive faecal samples were isolated by sheather's sucrose flotation method and picked from the concentrate using cover slips. genomic dna was extracted from 124 of the faecal samples that were positive for crypto ... | 2012 | 22797974 |
| molecular and phylogenetic approaches for assessing sources of cryptosporidium contamination in water. | the high sequence diversity and heterogeneity observed within species or genotypes of cryptosporidium requires phylogenetic approaches for the identification of novel sequences obtained from the environment. a long-term study on cryptosporidium in the agriculturally-intensive south nation river watershed in ontario, canada was undertaken, in which 60 sequence types were detected. of these sequence types 33 were considered novel with no identical matches in genbank. detailed phylogenetic analysis ... | 2012 | 22841595 |
| multilocus sequence subtyping and genetic structure of cryptosporidium muris and cryptosporidium andersoni. | in this study, nine c. muris and 43 c. andersoni isolates from various animals in china were subtyped by a multilocus sequence typing (mlst) tool. dna sequence analyses showed the presence of 1-2 subtypes of c. muris and 2-6 subtypes of c. andersoni at each of the four loci (ms1, ms2, ms3, and ms16), nine of which represented new subtypes. altogether, two c. muris and 10 c. andersoni mlst subtypes were detected. linkage disequilibrium analysis indicated although the overall population structure ... | 2012 | 22937094 |
| [detection of immunity and antioxidant indexes in dairy cows infected with cryptosporidium]. | to detect the immune status and antioxidant system indexes of cows infected with cryptosporidium. | 2012 | 23072162 |
| spatiotemporal analysis of cryptosporidium species/genotypes and relationships with other zoonotic pathogens in surface water from mixed-use watersheds. | nearly 690 raw surface water samples were collected during a 6-year period from multiple watersheds in the south nation river basin, ontario, canada. cryptosporidium oocysts in water samples were enumerated, sequenced, and genotyped by detailed phylogenetic analysis. the resulting species and genotypes were assigned to broad, known host and human infection risk classes. wildlife/unknown, livestock, avian, and human host classes occurred in 21, 13, 3, and <1% of sampled surface waters, respective ... | 2012 | 23124241 |
| infectivity of cryptosporidium andersoni kawatabi type relative to the small number of oocysts in immunodeficient and immunocompetent neonatal and adult mice. | cryptosporidium andersoni is a protozoan parasite found in many countries that invades the stomachs of primarily adult cattle. unlike the isolates of c. andersoni in cattle from other countries, c. andersoni isolates from japanese cattle can infect mice and were identified as a novel type and later defined as c. andersoni kawatabi type. the biological characteristics of c. andersoni kawatabi type have not yet been well documented. in the present study, we assess the infectivity of this type isol ... | 2013 | 23146684 |
| novel quantitative taqman real-time pcr assays for detection of cryptosporidium at the genus level and genotyping of major human and cattle-infecting species. | development of taqman mgb real-time pcr assays for quantitative typing of major cattle and human-pathogenic cryptosporidium species. | 2013 | 23230846 |
| molecular epidemiology of cryptosporidium in livestock animals and humans in the ismailia province of egypt. | the zoonotic potential of cryptosporidium was studied in one of the most densely populated provinces of egypt regarding livestock and people. in a representative survey, faecal samples from cattle, buffalo and stool samples from diarrhoeic children (<10 years) were investigated. parameters assumed to be related to cryptosporidiosis were recorded for animals and children. animal samples (804) were examined by the copro-antigen rida(®)quick test, followed by pcrs targeting the 18s rdna and gp60 ge ... | 2012 | 23305974 |
| first description of heterogeneity in 18s rrna genes in the haploid genome of cryptosporidium andersoni kawatabi type. | the apicomplexan cryptosporidium andersoni, is a species of gastric cryptosporidium, is frequently detected in older calves and adult cattle. genotyping analyses based on 18s ribosomal rna gene sequences have been performed on a novel c. andersoni genotype, namely the kawatabi type, and the oocysts were classified into two distinct groups genotypically: type a (the sequence in genbank) and type b (with a thymine nucleotide insertion not in type a). this study analyzed 3775 cattle at a slaughterh ... | 2013 | 23369454 |
| pathological features of cryptosporidium andersoni-induced lesions in scid mice. | to assess the infectivity and the istopathological features of cryptosporidium andersoni (c. andersoni) in laboratory animals, scid mice were orally inoculated with oocysts of c. andersoni. starting one week after inoculation, the scid mice began shedding oocysts, and this continued for ten weeks. histopathologically, myriads of c. andersoni were observed on the apical surface of the epithelium in the gastric pit of the glandular stomach. there were few lesions in the gastric epithelium except c ... | 2013 | 23499882 |
| genotyping cryptosporidium andersoni in cattle in shaanxi province, northwestern china. | the present study examined the prevalence and genotypes of cryptosporidium andersoni in cattle in shaanxi province, china. a total of 2071 fecal samples (847 from qinchuan cattle and 1224 from dairy cattle) were examined for the presence of cryptosporidium oocysts, and 70 samples (3.4%) were c. andersoni-positive and those positive samples were identified by pcr amplification of the small subunit ribosomal rna (ssu rrna) and the cryptosporidium oocyst wall protein (cowp) genes. c. andersoni was ... | 2013 | 23560072 |
| occurrence and molecular identification of cryptosporidium species isolated from cattle in poland. | cryptosporidiosis is a disease reported in both humans and animals and is caused by a protozoan parasite cryptosporidium. in most cases calves are infected by different cryptosporidium species adapted to this animal host. most infections are subclinical; however in some cases the watery diarrhea appears. the rapid development of new molecular diagnostic tools has provided an opportunity for better recognition of cryptosporidium species and genotypes isolated from infected hosts. the aim of this ... | 2013 | 23566407 |
| the lesser egyptian gerbil (gerbillus gerbillus) is a suitable host for the long-term propagation of cryptosporidium andersoni. | we describe the course of infection of cryptosporidium andersoni li03, originally isolated from cattle, in outbred gerbillus gerbillus (lesser egyptian gerbil), meriones unguiculatus (mongolian gerbil), and meriones tristrami (tristram's jird). while both meriones spp. partially cleared the infection and shed a low number of oocysts (less than 15,000 oocysts per gram (opg)), chronic infection with a mean infection intensity reaching 200,000 opg was observed in g. gerbillus. these data suggest th ... | 2013 | 23644354 |
| a new genotype of cryptosporidium from giant panda (ailuropoda melanoleuca) in china. | fifty-seven fecal samples were collected from giant pandas (ailuropoda melanoleuca) in the china conservation and research centre for the giant panda (ccrcgp) in sichuan and examined for cryptosporidium oocysts by sheather's sugar flotation technique. an 18-year-old male giant panda was cryptosporidium positive, with oocysts of an average size of 4.60×3.99 μm (n=50). the isolate was genetically analyzed using the partial 18s rrna, 70 kda heat shock protein (hsp70), cryptosporidium oocyst wall pr ... | 2013 | 23810821 |
| a coprological survey of parasites of wild carnivores in ireland. | the increasing movement of people to wilderness areas, shrinking of wildlife habitats and the resulting urbanisation of wildlife has led to growing concerns about the transfer of parasitic diseases, particularly from contaminated faeces. faecal samples from wild carnivores in ireland were examined for the presence of protozoan and nematode parasites. red fox (vulpes vulpes) samples (n = 91) were positive for uncinaria stenocephala (38%), eucoleus aerophilus (26%), toxocara canis (20%), trichuris ... | 2013 | 23900557 |
| prevalence and characterization of cryptosporidium spp. in dairy cattle in nile river delta provinces, egypt. | molecular characterizations of cryptosporidium spp. in dairy cattle in industrialized nations have mostly shown a dominance of cryptosporidium parvum, especially its iia subtypes in pre-weaned calves. few studies, however, have been conducted on the distribution of cryptosporidium species and c. parvum subtypes in various age groups of dairy cattle in developing countries. in this study, we examined the prevalence and molecular characteristics of cryptosporidium in dairy cattle in four nile rive ... | 2013 | 24036320 |
| infectivity of cryptosporidium andersoni and cryptosporidium muris to normal and immunosuppressive cynomolgus monkeys. | cryptosporidium andersoni and cryptosporidium muris infections have been found in the mice and/or cattle. the oocysts of c. andersoni and c. muris have been sporadically detected in human feces, but the infectious capacity and features have been unknown, because of the scarcity of reports involving human infections. to assess the infectivity and the clinical and pathological features of c. andersoni and c. muris in primates, an experimental infectious study was conducted using cynomolgus monkeys ... | 2014 | 24132296 |
| survey on gastrointestinal parasites and detection of cryptosporidium spp. on cattle in west java, indonesia. | to evaluate the presence of gastrointestinal parasites on cattle in indonesia because the prevalence of parasites varies between countries depending on the terrain surrounding livestock farms and investigations in indonesia have never been performed. | 2014 | 24507639 |
| multilocus genotype and subtype analysis of cryptosporidium andersoni derived from a bactrian camel (camelus bactrianus) in china. | fecal specimens from two bactrian camels were collected in the ya'an city zoo of china and were examined for cryptosporidium by centrifugal flotation. one specimen was found to be parasitized by cryptosporidium via microscopy, and the oocysts were measured to have an average size of 7.03 × 5.50 μm (n > 50). the isolate was genotyped by polymerase chain reaction (pcr) amplification and dna sequence analysis of the partial 18s rrna, cowp, and a135 genes, and was confirmed to be cryptosporidium and ... | 2014 | 24676462 |
| cryptosporidium species in sheep and goats from papua new guinea. | species of cryptosporidium are extensively recognised as pathogens of domesticated livestock and poultry, companion animals, wildlife, and are a threat to public health. little is known of the prevalence of cryptosporidium spp. in humans, domesticated animals or wildlife in papua new guinea (png). the aim of the present study was to screen sheep and goats for cryptosporidium using molecular tools. a total of 504 faecal samples were collected from sheep (n=276) and goats (n=228) in village, gover ... | 2014 | 24703974 |
| prevalence, molecular characterization and zoonotic potential of cryptosporidium spp. in goats in henan and chongqing, china. | to estimate the prevalence and public health significance of cryptosporidiosis in goats in china, 1265 fecal samples from seven farms in henan province and chongqing city were examined for cryptosporidium oocysts. the overall infection rate of cryptosporidium spp. was 3.48% (44/1256). significant difference was observed among age groups, with the post weaned kids having the highest infection rate (4.58%; ρ<0.01). cryptosporidium spp. were characterized by pcr-restriction fragment length polymorp ... | 2014 | 24721256 |