Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| nucleoside triphosphate phosphohydrolase associated with cytoplasmic polyhedrosis virus. | nucleoside triphosphate phosphohydrolase ec 3.6.1.15 activity was found to be included in silkworm cytoplasmic polyhedrosis (cp) virus, which synthesizes mrna carrying the 5'-terminal modification. this enzyme releases orthophosphate from the gamma-position in a nucleoside triphosphate, leaving nucleoside diphosphate. the rate of hydrolysis of atp is faster than that of any other ribonucleoside triphosphate. deoxy atp is hydrolyzed rather faster than atp. however, polynucleotides carrying tripho ... | 1977 | 14944 |
| a blocked structure at the 5' terminus of mrna from cytoplasmic polyhedrosis virus. | 1975 | 163011 | |
| the process of formation of the 5' -terminal modified structure in messenger rna of cytoplasmic polyhedrosis virus. | 1976 | 178543 | |
| importance of 5'-terminal blocking structure to stabilize mrna in eukaryotic protein synthesis. | the 7-methylguanylic acid residue confronting the 5'-terminal nucleotide of mrna through two pyrophosphate linkages was completely removed by tobacco pyrophosphatase from mrnas of cytoplasmic polyhedrosis virus, tobacco mosaic virus (viral rna), and globin without any scission in the inner part of the rna chain. protein synthesis ability in a wheat germ cell-free system was lost after this treatment of all three kinds of mrna. the initiation complexes for protein synthesis of these three rnas we ... | 1977 | 197518 |
| inhibitory effect of methylated derivatives of guanylic acid for protein synthesis with reference to the functional structure of the 5'-'cap' in viral messenger rna. | guanylic acid modified variously with methyl groups on base or sugar moieties were synthesized chemically and their inhibitory effects on protein synthesis were tesetd in a wheat germ cell-free system using mrnas from cytoplasmic polyhedrosis virus and tobacco mosaic virus. the confronting dinucleotide m7g5' pppa that corresponds to the most simple 'cap' structure of an eukaryotic mrna is a strong inhibitor of protein synthesis, but non-methylated g5' pppa or g5' ppa is not inhibitory. the stron ... | 1979 | 226144 |
| molecular weights of the rna genome segments of a cytoplasmic polyhedrosis virus determined by a new comparative approach. | 1977 | 319594 | |
| transcription of double-stranded rna by escherichia coli dna-dependent rna polymerase. | double-stranded rna of some virus genomes can be used as template for the dna-dependent rna polymerase purified from escherichia coli. the rna synthesis requires all four nucleoside triphosphates and manganese ions and is dependent on the presence of sigma subunit. the reaction is inhibited by rifampicin, streptolydigin and ethidium bromide, but not by dnase and actinomycin d which does not bind to double-stranded rna. the template activity of double-stranded rna from various viruses is differen ... | 1977 | 320006 |
| electron microscope observations on the development of cytoplasmic polyhedrosis virus in scotogramma trifolii rott. (lepidoptera, noctuidae). | 1977 | 329953 | |
| [in vivo and in vitro studies of a cytoplasmic polyhedrosis virus isolated from euxoa scandens, riley (noctuidae, agrotinae)(author's transl)]. | a virus isolated from the larvaes of euxoa scandens (noctuidae, agrotinae) was studied by histological and histochemical techniques, and by electron microscopic observation of virus suspensions and ultrathin sections of infected tissues. our studies demonstrated that the virus in icosahedral in shape, measures 70 nm and belongs to the cytoplasmic polyhedrosis virus group. only the midgut cells of the larvae were found to be infected and the virus particles were localized in inclusion bodies most ... | 1977 | 336008 |
| reproducible alteration of cytoplasmic polyhedrosis virus double-stranded rna genome patterns on laboratory passage. | 1978 | 339515 | |
| "pretranscriptional capping" in the biosynthesis of cytoplasmic polyhedrosis virus mrna. | the in vitro synthesis of cytoplasmic polyhedrosis virus (cpv) mrna was previously shown to be dependent upon the presence of the methyl donor s-adenosylmethionine (adomet). we now find that the competitive inhibitor of methylation, s-adenosylhomocysteine (adohcy), also stimulates cpv mrna synthesis efficiently, resulting in the synthesis of viral mrnas containing 5'-terminal gpppa and ppa, rather than m(7)gpppam as observed with adomet. in addition to adohcy, other adomet analogues, including s ... | 1978 | 349555 |
| the multicomponent genome of a different cytoplasmic polyhedrosis virus isolated from heliothis armigera. | the genome of a new cytoplasmic polyhedrosis virus (cpv) isolated from heliothis armigera has been shown to consist of 10 double-stranded rna segments, as in other cpvs, but the genome profile on page bears no resemblance to previously described cpvs isolated from h.armigera. | 1978 | 361635 |
| a new host, simulium argyreatum meig., for the cytoplasmic polyhedrosis virus of blackflies in czechoslovakia. | the cytoplasmic polyhedrosis virus (cpv) from the midgut of blackfly larvae is recorded from a population of simulium argyreatum in czechoslovakia. a 7.7% infection of 2766 sampled larvae was manifested by white spots in their body. 85% of the white-spotted larvae were infected with cpv when sampled in june. in august 9% of 1377 larvae had white spots but only 10.6% of them were with cpv. out of 823 collected pupae, only 3 imperfect pupae had the virus in apparent form. other infections producin ... | 1978 | 369963 |
| the blocked and methylated 5' terminal sequence of a specific cellular messenger: the mrna for silk fibroin of bombyx mori. | the messenger rna for silf fibroin, laveled with 32po4 and methyl-3h l-methionine, was purified to near homogeneity from the posterior silk gland of the sildworm bombyx mori, and the sequence of a methylated, rnaase t2-resistant structure was determined. this sequence is similar structurally to 5' terminal blocked and methylated sequences found on the total populations of polyadenylated eucaryotic cellular and certain viral mrnas. the rnaase t2-resistant oligomer from fibroin mrna was cleaved by ... | 1976 | 947545 |
| a cytoplasmic polyhedrosis virus from the larval blackflies cnephia mutata and prosimulium mixtum (diptera:simuliidae). | 1975 | 1090682 | |
| iridovirus and cytoplasmic polyhedrosis virus in the freshwater daphnid simocephalus expinosus. | 1975 | 1090849 | |
| transmission, diagnosis, and control of cytoplasmic polyhedrosis virus in colonies of heliothis virescens. | 1975 | 1097538 | |
| isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques. | procedures are described for the separation and purification of cytoplasmic polyhedrosis viruses obtained from multiply infected heliothis armigera larvae. separation was achieved by differential centrifugation and density gradient zone electrophoresis followed by complexing with nuclear polyhedrosis virus specific antibody. the yield of cytoplasmic polyhedrosis virus was increased by passage in larvae reared on synthetic media. | 1975 | 1098037 |
| antibodies ot double-stranded rna: specificity and serum nucleases. | an antiserum was prepared in rabbits to the synthetic double-stranded ribonucleic acid (ds rna) poly ri:rc. using a liquid-phase radioimmunoassay, the antiserum cross-reacted with a natural ds rna isolated from the cytoplasmic polyhedrosis virus of the silkworm, binding 95% of the rna at a 1 : 20 serum dilution. preliminary tests of the specificity of the antiserum showed that it did not bind single-stranded rna (ss rna) or deoxyribonucleic acid (dna), but also revealed that the serum contained ... | 1975 | 1107429 |
| isolation of cdna clones coding for humoral lectin of silkworm, bombyx mori, larvae. | in the hemolymph of the silkworm, bombyx mori, lectin with hemagglutinating activity against sheep red blood cells increases at larval-larval ecdysis and at spinning stage (suzuki and natori, 1983) and is induced by infection with cytoplasmic polyhedrosis virus. a bombyx lectin polypeptide with molecular weight approx 280k is responsible for hemagglutinating activity, since antiserum raised against this polypeptide inhibited hemagglutinating activity. the site of synthesis of bombyx lectin was d ... | 1992 | 1541849 |
| entomopathogens of phlebotomine sand flies: laboratory experiments and natural infections. | the susceptibility of different geographical strains of phlebotomus papatasi to a cytoplasmic polyhedrosis virus (cpv) was determined experimentally by feeding polyhedra to larvae. of the indian p. papatasi, 15.6% became infected, whereas egyptian p. papatasi were mostly refractory. infection rates were not augmented in colony flies from the jordan valley, 23.8% of which were naturally infected with cpv. the infectivity of serratia marcescens and beauvaria bassiana to p. papatasi were determined ... | 1991 | 1783777 |
| x-ray powder pattern analysis of cytoplasmic polyhedrosis virus inclusion bodies. | cytoplasmic polyhedrosis virus is an insect reovirus which is occluded in crystalline inclusion bodies that form in the mid-gut of certain insects. inclusion bodies of cytoplasmic polyhedrosis virus from bombyx mori were isolated and purified. these crystalline bodies, about 1-3 microns in linear size, were compacted in a capillary tube while immersed in buffer. x-ray diffraction photographs showed powder rings, extending to 8.2 a resolution, which could be indexed with a cell measuring a = b = ... | 1991 | 1984644 |
| cytoplasmic polyhedrosis virus classification by electropherotype; validation by serological analyses and agarose gel electrophoresis. | serological analyses of several different cytoplasmic polyhedrosis viruses (cpvs), including two type 1 cpvs from bombyx mori, type 1 cpv from dendrolimus spectabilis, type 12 cpv from autographa gamma, type 2 cpv from inachis io, type 5 cpv from orgyia pseudotsugata and type 5 cpv from heliothis armigera, demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsrna electropherotypes. the dsrnas of these viruses w ... | 1989 | 2499658 |
| nucleotide sequence of the polyhedrin gene of euxoa scandens cytoplasmic polyhedrosis virus (escpv). | the double-stranded rna genome of euxoa scandens cytoplasmic polyhedrosis virus (escpv) was reversely transcribed to the double-stranded dna and cloned into pibi30. the complete nucleotide sequence of cloned genome segment 10, which encodes the virus polyhedrin polypeptide, was determined. the escpv polyhedrin gene consists of 881 bp and possesses an open reading frame that codes for a polypeptide of 269 amino acids (mw 30.1k), consistent with an apparent mw of 30k determined by sds-page for pur ... | 1989 | 2650462 |
| nucleotide sequence of the polyhedrin gene of bombyx mori cytoplasmic polyhedrosis virus a strain with nuclear localization of polyhedra. | bombyx mori cytoplasmic polyhedrosis virus (bmcpv) strain h produces many hexahedral polyhedra (inclusion bodies) in the cytoplasm of insect midgut epithelial cells. the mutant a strain, however, produces polyhedra in the nucleus. we determined cdna sequences of the polyhedrin genes, the smallest of the 10 genome segments, of these two strains. the polyhedrin genes of the h and a strains were 944 bp long, and encoded polypeptides of 248 amino acids (mr 28,500) and 252 amino acids (mr 29,000), re ... | 1989 | 2661724 |
| electrophoretic separation of the plus and minus strands of rotavirus sa11 double-stranded rnas. | the genome of the rotaviruses consists of eleven segments of completely double-stranded rna (dsrna). to provide a method for separating and identifying the complementary plus and minus strand rnas within these segments, we have characterized their migration patterns under denaturing conditions on agarose-urea gels. virion-derived 3h-labelled dsrnas were resolved by electrophoresis on a polyacrylamide gel and the individual genome segments recovered by electroelution. upon electrophoresis in a lo ... | 1986 | 3016018 |
| molecular cloning and characterization of cytoplasmic polyhedrosis virus polyhedrin and a viable deletion mutant gene. | the double-stranded rna genome of bombyx mori cytoplasmic polyhedrosis virus (cpv) was converted to double-stranded dna and cloned into plasmid pbr322. the complete nucleotide sequence of cloned genome segment 10, which encodes virus polyhedrin polypeptide, was determined. the cpv polyhedrin gene consists of 942 based pairs and possesses a long open reading frame that codes for a polypeptide of 248 amino acids (molecular weight, 28,500), consistent with an apparent molecular weight of 28,000 pre ... | 1988 | 3275433 |
| coupled transcription-translation of silkworm cytoplasmic polyhedrosis virus injected into oocytes of the frog, xenopus laevis. | the cytoplasmic polyhedrosis virus of the silkworm, bombyx mori, contains a ten-segmented, double-stranded rna genome and five species of polypeptides, v1 (mr 146,000), v2 (mr 140,000), v3 (mr 128,000)), v4 (mr 62,000), and v5 (mr 32,000). the virus contains an rna-dependent rna polymerase that transcribes the duplex genome rna to form mrna either in the infected animals or under appropriate conditions in vitro. we co-microinjected the virus, [alpha-32p]gtp and actinomycin d into oocytes of the ... | 1988 | 3283114 |
| the protein subunits of the double-stranded rna dependent rna polymerase and methyltransferase of the cytoplasmic polyhedrosis virus of silkworm, bombyx mori. | the particles of cpv of silkworm contain double-stranded rna polymerase and methyltransferase. it was reported in a previous paper that the genome-enzyme complex could be isolated. the genome-enzyme complex shows high enzyme activity of rna polymerase and methyltransferase in spite of the fact that it consists of only 5 percent of the protein. in order to clarify the protein subunits of the rna polymerase and methyltransferase, two methods were adopted. the sds-polyacrylamide gel electrophoretog ... | 1986 | 3299695 |
| a new method for extracting circular and supercoiled genome segments from cytoplasmic polyhedrosis virus. | the genome of a cytoplasmic polyhedrosis virus (cpv) consisting of 10 segments was extracted by a new two-step extraction method. the results from polyacrylamide gel electrophoresis, phenol and diepoxybutane treatment and electron microscopic examination indicated the extracted genome segments to have circular and supercoiled structures as genome-protein(s) complexes. multiple cycles of the transcription of each segmented genome of cpv may take place on the circular structure of the segment. | 1986 | 3539959 |
| gene assignments of a cytoplasmic polyhedrosis virus (type 2) from nymphalis io. | the 10 double stranded (ds) rnas from cytoplasmic polyhedrosis virus (type 2) from nymphalis io have been separated by polyacrylamide gel electrophoresis and fractionated into separate genes. separate and total dsrna were denatured using methyl mercury, and translated in a rabbit reticulocyte lysate. translation of unfractionated rna generated proteins that comigrated with virion and polyhedral structural proteins and six major non-structural proteins. translation of individual rnas, demonstrate ... | 1985 | 3892233 |
| a cytoplasmic polyhedrosis virus of triphaena pronuba (l.) (lepidoptera, noctuidae). | 1970 | 4099856 | |
| electron microscopic study on rna of cytoplasmic polyhedrosis virus of the silkworm. | 1969 | 4308564 | |
| characterization of the genome of cytoplasmic polyhedrosis virus. | it is possible to oxidize in situ the 3'-terminals of the double-stranded ribonucleic acid (rna) contained in structurally intact cytoplasmic polyhedrosis virus. the number of 3'-terminals subsequently found by reduction with tritiated sodium borohydride was equivalent to the number observed in rna first isolated from the virus and then oxidized and reduced. the viral rna appears to occur in 10 distinct polynucleotide segments present in equimolar amounts. | 1971 | 4329560 |
| enzyme activities associated with cytoplasmic polyhedrosis virus from bombyx mori. ii. comparative studies. | 1973 | 4366479 | |
| 5'-terminal nucleotide sequences of the double-stranded rna of silkworm cytoplasmic polyhedrosis virus. | 1974 | 4369455 | |
| the 5'-terminal nucleotide sequences of the double-stranded rna of human reovirus. | the 5'-terminal nucleotide sequences of human reovirus double-stranded rna were determined after labeling the rna with [(32)p]phosphate by polynucleotide kinase. the 5' terminal were labeled to only a limited extent prior to sequential oxidation, beta-elimination, and phosphomonoesterase treatment, indicating that the terminal phosphates were in a modified, blocked configuration. each genome segment, after removing the blocking group, contained the same two 5'-terminal sequences: gpapup in one c ... | 1974 | 4530278 |
| a cytoplasmic polyhedrosis virus in midgut cells of anopheles stephensi and in the sporogonic stages of plasmodium berghei yoelii. | although it has been known for some time that nuclear and cytoplasmic polyhedrosis viruses may infect the larval stages of mosquitos capable of transmitting mammalian malaria this paper reports for the first time the presence of a cytoplasmic polyhedrosis virus in adult anopheles stephensi. the virus was shown to be present in sporogonic stages of plasmodium berghei yoelii with which the mosquitos were infected. it is possible that other viruses may affect both vector and malaria parasites. furt ... | 1972 | 4557906 |
| [rna polymerase associated with double-stranded rna virus, with special reference to rna polymerase associated with cytoplasmic polyhedrosis virus of the silkworm]. | 1973 | 4567925 | |
| transcription of double-stranded rna in cytoplasmic polyhedrosis virus in vtro. | 1973 | 4574875 | |
| single-stranded rna synthesis in vitro by the rna polymerase associated with cytoplasmic polyhedrosis virus containing double-stranded rna. | 1973 | 4582169 | |
| the synthesis of virus-specific single-stranded rna in larvae of bombyx mori infected with a cytoplasmic polyhedrosis virus. | 1973 | 4589642 | |
| replication of a cytoplasmic polyhedrosis virus in an established cell line of trichoplusia in cells. | 1974 | 4598710 | |
| 5'-terminal structure of messenger rna transcribed by rna polymerase of silkworm cytoplasmic polyhedrosis virus containing double-stranded rna. | 1974 | 4605463 | |
| the structural proteins and rna components of a cytoplasmic polyhedrosis virus from nymphalis io (lepidoptera: nymphalidae). | 1974 | 4612108 | |
| sequence homology studies between the double-stranded rna genomes of cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus strains 1, 2 and 3. | 1974 | 4615076 | |
| biochemical properties of polyhedra and virus particles of the cytoplasmic polyhedrosis virus of bombyx mori. | 1974 | 4619114 | |
| a radioimmunoassay for the cytoplasmic polyhedrosis virus from bombyx mori. | 1974 | 4619115 | |
| comparison of the structure and polypeptide composition of three double-stranded ribonucleic acid-containing viruses (diplornaviruses): cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus. | iodination of reovirus, cytoplasmic polyhedrosis virus (cpv), and wound tumor virus (wtv), and their respective subviral forms, followed by analysis of the labeled polypeptides by using polyacrylamide gel electrophoresis, has been used to compare the protein contents of these three diplornaviruses. this approach, when combined with electron microscopy and buoyant density determinations, appears capable of localizing individual polypeptides in some of the viral and subviral forms. cpv (p = 1.435 ... | 1972 | 4636394 |
| comparison of the 3' termini of discrete segments of the double-stranded ribonucleic acid genomes of cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus. | the 3' terminal nucleosides of the isolated components of double-stranded ribonucleic acids of reovirus, wound tumor virus, and cytoplasmic polyhedrosis virus were determined by labeling with tritiated sodium borohydride. all wound tumor virus and cytoplasmic polyhedrosis virus components appear to contain approximately equal amounts of u(oh) and c(oh) termini. reovirus segments have essentially only c(oh) termini. | 1972 | 4636396 |
| surface topography and shapes of polyhedral inclusion bodies of the cytoplasmic polyhedrosis virus. | 1973 | 4696220 | |
| identity of the 3'-terminal sequences in ten genome segments of silkworm cytoplasmic polyhedrosis virus. | 1973 | 4742779 | |
| [studies on the cytoplasmic polyhedrosis virus of silkworms]. | 1968 | 4881543 | |
| cytoplasmic polyhedrosis virus (cpv) of malacosoma disstria: rna polymerase activity in purified free viroin. | 1972 | 5018698 | |
| the 3'-termini of the genome rna segments of silkworm cytoplasmic polyhedrosis virus. | 1972 | 5022191 | |
| amino acid composition of proteins extracted from virions of cytoplasmic-polyhedrosis virus. | 1971 | 5092822 | |
| synthesis of ribonucleic acid resistant to actinomycin d in silkworm midguts infected with the cytoplasmic-polyhedrosis virus. | 1971 | 5092827 | |
| presence of cytoplasmic polyhedrosis virus in the hemolymph of heliothis virescens larvae and adults. | 1971 | 5106447 | |
| hemagglutination with cytoplasmic-polyhedrosis virus of the silkworm, bombyx mori. | 1969 | 5344296 | |
| nuclear-polyhedrosis and cytoplasmic-polyhedrosis virus infections in louisiana mosquitoes. | 1969 | 5385139 | |
| the site of rna synthesis of a cytoplasmic-polyhedrosis virus (cpv) in malacosoma disstria. | 1970 | 5449739 | |
| the isolation of cytoplasmic polyhedrosis virus from the white-marked tussock moth, orgyia leucostigma (smith). | 1970 | 5484060 | |
| rna in midgut of tussock moth, orgyia leucostigma, infected with cytoplasmic-polyhedrosis virus. | 1970 | 5491276 | |
| occluded and free virions in midgut cells of malacosoma disstria infected with cytoplasmic-polyhedrosis virus (cpv). | 1970 | 5501205 | |
| properties of rna from cytoplasmic-polyhedrosis virus of the white-marked tussock moth, orgyia leucostigma. | 1970 | 5501206 | |
| cytoplasmic polyhedrosis virus: rna synthesized in vivo and in vitro in infected midgut. | 1971 | 5545513 | |
| resistance of the alfalfa caterpillar, colias eurytheme, at high temperatures to a cytoplasmic-polyhedrosis virus and thermal inactivation point of the virus. | 1968 | 5649247 | |
| ultrastructure of a cytoplasmic polyhedrosis virus affecting the monarch butterfly, danaus plexippus. i. development of virus and normal polyhedra in the larva. | 1968 | 5709882 | |
| an histopathological study involving a cytoplasmic-polyhedrosis virus of the salt-marsh caterpillar, estigmene acrea. | 1968 | 5716591 | |
| chromatographic studies on rna synthesis in the midgut of the silkworm, bombyx mori, infected with a cytoplasmic-polyhedrosis virus. | 1968 | 5716594 | |
| constitution of the ribosomal fraction from the forest-tent-caterpillar (malacosoma disstria) midgut infected with a cytoplasmic-polyhedrosis virus. | 1968 | 5716598 | |
| metabolic changes in diseased insects. ii. radioautographic studies on dna and rna synthesis in nuclear-polyhedrosis and cytoplasmic-polyhedrosis virus infections. | 1968 | 5725007 | |
| purification of a cytoplasmic-polyhedrosis virus of the silkworm, bombyx mori. | 1969 | 5777835 | |
| ineffectiveness of a microsporidian and a granulosis virus in activating a cytoplasmic polyhedrosis virus in the alfalfa caterpillar, colias eurytheme. | 1969 | 5777841 | |
| electron-microscopic observations on the maturation of a cytoplasmic-polyhedrosis virus. | 1969 | 5804153 | |
| electron microscopic study of rna of cytoplasmic polyhedrosis virus of the silk worm. | 1969 | 5812159 | |
| resistance to peroral infection by the cytoplasmic-polyhedrosis virus in the silkworm, bombyx mori (linnaeus). | 1965 | 5827559 | |
| free amino acids in the hemolymph and midgut epithelium of the silkworm, bombyx mori (linnaeus), infected with cytoplasmic-polyhedrosis virus. | 1965 | 5890108 | |
| a cytoplasmic-polyhedrosis virus, smithiavirus pectinophorae sp. n. of the pink bollworm, pectinophora gossypiella (saunders). | 1966 | 5905538 | |
| the development of a cytoplasmic polyhedrosis virus in the spruce budworm, choristoneura fumiferana (clemens). | 1966 | 5929158 | |
| studies of two strains of cytoplasmic-polyhedrosis virus. | 1966 | 5939382 | |
| ribonucleic acid extracted from the cytoplasmic-polyhedrosis virus of the silkwom, bombyx mori. | 1967 | 6073676 | |
| the process of virus assembly in insect virus mixed infections. | interference occurred upon infection of the cabbage moth caterpillars (mamestra brassicae l.) with a mixture of nuclear polyhedrosis virus (npv) and cytoplasmic polyhedrosis virus (cpv), resulting in the impairment of virus assembly, and formation of abnormal nucleocapsids. at the same time protein supercapsids were produced normally, but contained no infectious virions. when insects were infected with related viruses, the virions developed as usual, but the protein supercapsids revealed abnorma ... | 1983 | 6139945 |
| electron microscopic studies of bacteriophage phi x174 intact and "eclipsing' particles, and the genome by the staining, and shadowing method. | bacteriophage phi x174 particles were observed with a new method, a combination of staining and shadowing. previously we used this technique to obtain clear visualization of the shape of cytoplasmic-polyhedrosis virus, especially the spikes of this particle. the phi x174 particle was observed to be an icosahedral particle of 25 nm in diameter carrying a spike at each vertex. the spike was a pentagonal frustum with a base of 9 nm and a height of 6 nm. when the phage was treated with calcium on th ... | 1981 | 6168647 |
| a mode of action of rnase ci toward the various rnas. | a mode of action of rnase ci toward the various native rnas was examined. the mixture of trna from thermus thermophilus hb8 were exhaustively cleaved by rnase ci to produce 90% of acid-soluble fraction. among the minor nucleotides s2t were recognized by this enzyme. rnase ci generates a linear viroid molecule by introducing one single nick into viroid circular molecule. double stranded rna from rna virus, such as cytoplasmic polyhedrosis virus (cpv) from silk worm, was cleaved at some specific s ... | 1982 | 6191284 |
| midgut and viral associated proteases of heliothis armigera. | the role played by the gut juice of insects in the infective process of insect viruses was examined. analysis of larval gut extract of heliothis armigera by sds-page revealed protease activity associated with components of molecular weights 48,000 and 94,000. proteases were found to be associated with occlusion bodies and virions of both nuclear polyhedrosis virus (npv) and cytoplasmic polyhedrosis virus (cpv) infecting h. armigera. cpv occlusion bodies were dissolved by gut juice extract at ph ... | 1983 | 6337972 |
| the effects of s-adenosyl methionine (adomet) and its analogues on the control of transcription and translation in vitro of the mrna products of two cytoplasmic polyhedrosis viruses. | s-adenosyl methionine (adomet) and several structurally related compounds were added to in vitro systems for the synthesis of single-stranded rna by cytoplasmic polyhedrosis virus (cpv) types 1 and 2. the effects of these compounds were examined on the level of transcription and methylation of the rna products. of the compounds tested, five increased the polymerase activity in both viruses, the most effective being the d- and l-stereoisomers of s-adenosyl homocysteine (adohcy), and the least eff ... | 1983 | 6359667 |
| [cross transmission of a cytoplasmic polyhedrosis virus in the genus dendrolimus]. | 1983 | 6367220 | |
| chemical synthesis of the 5'-terminal part bearing cap structure of messenger rna of cytoplasmic polyhedrosis virus (cpv): m7g5'pppampg and m7g5'pppampgpu. | the 5'-terminal structures of mrna bearing the so-called 'cap' from cytoplasmic polyhedrosis virus (cpv), m7g5' pppampg and m7g5' pppampgpu, were first chemically synthesized. s,s-di(4-methoxyphenyl) n6-benzoyl-2'-o-methyladenosine 5'-phosphorodithioate ((ars) 2pabmz) was prepared by phosphorylation of the 5'-hydroxyl group of n6-benzoyl-2'-o-methyladenosine with s,s-di(4-methoxyphenyl) phosphorodithioate by tps. by the triester approach using (ars) 2pabmz as starting material, the protected din ... | 1984 | 6369253 |
| characterization of a cytoplasmic polyhedrosis virus infecting manduca sexta. | a cytoplasmic polyhedrosis virus (cpv) infecting manduca sexta was characterized. the genome consisted of ten equimolar rna segments ranging in estimated molecular mass from 0.7 to 3.84 mdaltons and with a total estimated molecular mass of 21.75 mdaltons . the sizes of the individual rna segments provisionally placed this cpv within the type 8 classification of payne and rivers. the polyhedrin protein had an estimated molecular mass of 28 kdaltons . six virion-associated proteins were observed, ... | 1984 | 6373659 |
| comparison of the virus-associated polymerases of cytoplasmic polyhedrosis virus types 1 and 2. | a detailed comparison was made of the virus-associated polymerase activities of cytoplasmic polyhedrosis virus (cpv) types 1 and 2 which had previously been shown to differ in their response to the methyl donor s-adenosyl-l-methionine (adomet). while the type 1 cpv polymerase was approximately twice as active as the type 2 cpv enzyme in the presence of adomet, temperature, ph and divalent cation optima of the two enzymes were similar. both viruses synthesized in vitro single-stranded rna copies ... | 1984 | 6384129 |
| similarity in the structure of cytoplasmic polyhedrosis virus, leafhopper a virus and fiji disease virus particles. | 1982 | 6754656 | |
| some physical characteristics of a cytoplasmic polyhedrosis virus of heliothis armigera. | a number of physical properties of a cytoplasmic polyhedrosis virus of heliothis armigera were determined. these included a buoyant density of 1.44 g/cm3, a diffusion coefficient of 0.73 x 10(-7) cm2/sec, a partial specific volume of 0.688 ml/g, and a sedimentation coefficient of 399s. three different methods were used to determine an average molecular weight of 47.4 x 10(6). | 1980 | 6987192 |
| titration of a cytoplasmic polyhedrosis virus by a tissue microculture assay: some applications. | a simple tissue microculture technique was developed for the titration of a cytoplasmic polyhedrosis virus (cpv) from euxoa scandens. the procedure was similar to the 50% tissue culture infectious dose assay, but a single infected cell, detected by the presence of cytoplasmic polyhedra, was scored rather than the degeneration of cell monolayers. the filtration of cpv suspensions resulted in decreased virus titers under certain conditions. this microculture assay was used to determine the effect ... | 1980 | 6987194 |
| s-adenosylmethionine and its analogs. structural features correlated with synthesis and methylation of mrnas of cytoplasmic polyhedrosis virus. | 1980 | 6991498 | |
| allosteric stimulatory effect of s-adenosylmethionine on the rna polymerase in cytoplasmic polyhedrosis virus. a model for the positive control of eukaryotic transcription. | the stimulatory effect of s-adenosylmethionine (adomet) on cytoplasmic polyhedrosis virus transcription in vitro has been studied in detail. adomet affects the initiation of transcription but not chain elongation. it is required for each reinitiation step during multiple transcription cycles. under limited conditions of synthesis in reaction mixtures depleted of utp and ctp, a greater amount of initiator oligonucleotides was formed in the presence of adomet than in its absence. these small oligo ... | 1981 | 7005216 |
| production of cytoplasmic polyhedrosis virus (cpv) polyhedra in a gamma irradiated lymantria dispar cell line. | lymantria dispar cells wee exposed to different doses of gamma radiation one hour after infection with cytoplasmic polyhedrosis virus (cpv). it was found that irradiated cells can produce infectious polyhedra. modifications in the structure and in the process of maturation of the polyhedra were noted. the number of polyhedra per cell increased significantly after cell irradiation at 10(4) and 10(5) rads but no change was noted after cell treatment at 10(2) rads. on the other hand, morphological ... | 1981 | 7023418 |
| separation of the plus and minus strands of cytoplasmic polyhedrosis virus and human reovirus double-stranded genome rnas by gel electrophoresis. | the complementary strands of most of the genome double-stranded rna segments of insect cytoplasmic polyhedrosis virus (cpv) and human reovirus are separated for the first time by agarose gel electrophoresis in in the presence of 7 m urea. cpv (+) strands and most reovirus (-) strands migrate faster than the corresponding strands of opposite polarity. glyoxal treatment, which modifies guanine residues and prevents g-c basepairing, results in a loss of strand resolution and concomitantly a signifi ... | 1981 | 7029468 |
| a unique class of compound, guanosine-nucleoside tetraphosphate g(5')pppp(5')n, synthesized during the in vitro transcription of cytoplasmic polyhedrosis virus of bombyx mori. structural determination and mechanism of formation. | two structurally different classes of oligonucleotides accumulate in vitro in cytoplasmic polyhedrosis virus (cpv) transcription mixtures in molar excess as compared to the completed rna products. the first class consists of oligonucleotides which correspond to the 5'-terminal sequence of the virus mrnas (referred to as initiator oligonucleotides). the major species of initiator oligonucleotides are (p)ppapg and (p)ppapgpn together with smaller amounts of homologous capped structures (furuichi, ... | 1982 | 7031064 |
| electrophoretic characterization of proteins and rna of cytoplasmic polyhedrosis virus (cpv) from euxoa scandens. | a cytoplasmic polyhedrosis virus (cpv) was isolated from euxoa scandens and propagated in vitro in lymantria dispar cells. the virions and polyhedra were analyzed by polyacrylamide gel electrophoresis. polyhedra produced in vivo and in vitro contained six polypeptides with identical relative mobilities while nonoccluded viral particles produced in vivo were composed of four polypeptides. the rna content of the purified virions extracted from infected midgut was resolved in ten segments with mole ... | 1981 | 7036955 |