Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| allozyme variation among biotypes of the brown planthopper nilaparvata lugens in the philippines. | allozyme variation was studied in three nilaparvata lugens biotypes infesting specific rice varieties and a biotype infesting a weed grass, leersia hexandra. of the 20 enzymes in n. lugens for which activity was noted, 9 were polymorphic. eleven enzyme loci were monomorphic for the same allele in all biotype populations; the rest were polymorphic for two or more alleles. the mean number of alleles per polymorphic locus was 2.3, while the mean number of alleles per locus was 1.5; heterozygosity r ... | 1991 | 1859353 |
| a reovirus in the brown planthopper, nilaparvata lugens. | a new virus, belonging to the reovirus group, was found in an apparently healthy colony of the brown planthopper, nilaparvata lugens, and was referred to as the nilaparvata lugens reovirus (nlrv). the virus was found in the cytoplasm of the insect cells, sometimes associated with tubular structures, which is one of the characteristic features in tissues infected with reoviruses. the virus was purified by carbon tetrachloride clarification, polyethylene glycol precipitation, differential and cscl ... | 1991 | 1919524 |
| ultrastructure of sensilla on the antennal pedicel of the brown planthopper nilaparvata lugens stal (insecta: homoptera). | scanning- and transmission electron-microscope studies of the sensilla of the pedicel of nilaparvata lugens have revealed an elaborate plaque organ and three structurally different types of trichoid hairs. each plaque organ is innervated by 120-150 neurons arranged in groups. the porous sensory cuticle is folded into finger-like projections and is surrounded by protective non-sensory denticles. trichoid sensilla differ in number of neurons, appearance of dendrites and arrangement of pores. type ... | 1983 | 6825165 |
| ultrastructure of sensory receptors on the labium of the rice brown planthopper. | the ultrastructure of the sensory receptors located on the labium of the rice brown planthopper is described; possible functions of individual receptors are suggested on morphological grounds. uniporous chemosensilla which may or may not possess a mechanoreceptive dendrite, domed multiporous chemosensilla, and mechanoreceptive pegs are present on the flattened labial tip. mechanoreceptive pegs are distributed over the labium. the fine structure of a multilobed sensillum located on either side of ... | 1983 | 6850772 |
| sensory receptors associated with the stylets and cibarium of the rice brown planthopper, nilapavarta lugens. | the stylets of nilapavarta lugens consist of two maxillae that interlock to form separate food and salivary ducts partially surrounded by two mandibles. the ultrastructure of the sensory innervation of the stylets is described. each maxilla possesses five neurones which extend to the tip of the stylet. the mandibles also contain five neurones, four of which are paired. the paired neurones comprise a shorter dendrite extending part of the way along the stylet and a longer one extending to the tip ... | 1983 | 6883435 |
| actions of nitromethylenes on an alpha-bungarotoxin-sensitive neuronal nicotinic acetylcholine receptor. | nine nitromethylene analogues were tested for their actions on insect neuronal nicotinic acetylcholine receptors (nachrs). microelectrode recordings were used to study the actions of nitromethylenes on the cell body of an identified cockroach (periplaneta americana) motor neurone, the fast coxal depressor (df) in the metathoracic ganglion. six nitromethylenes showed potent nachr agonist actions; others were without nachr agonist actions. five nitromethylenes competitively displaced bound [125i]- ... | 1995 | 7566494 |
| electrophoretic karyotype of intracellular yeast-like symbiotes in rice planthoppers and anobiid beetles. | chromosomal dna molecules of intracellular yeast-like symbiotes (yls) of three species of rice planthoppers and two species of anobiid beetles have been separated by pulsed-field gel electrophoresis. probable chromosome numbers of nilaparvata lugens, sogatella furcifera, and laodelphax striatellus, were 4, 4, and 5, respectively, and tentative genome sizes were 17.3, 17.6, and 20.1 mbp, respectively, based upon migration of individual chromosome-sized dna relative to the size standards of schizo ... | 1995 | 7722339 |
| phylogenetic position of yeast-like symbiotes of rice planthoppers based on partial 18s rdna sequences. | the rice planthoppers, nilaparvata lugens, sogatella furcifera, and laodelphax striatellus, harbor intracellular yeast-like symbiotes (yls) in the fat body. the yls are obligated endosymbiotes and are transovarially inherited. nucleotide sequences of the 18s rrna genes from the yls were analyzed by direct sequencing. the yls of the three species of planthoppers were monophyletic and their taxonomic positions were in the class pyrenomycetes in the subphylum ascomycotina. | 1995 | 7787846 |
| nonpathogenic nilaparvata lugens reovirus is transmitted to the brown planthopper through rice plant. | nilaparvata lugens reovirus (nlrv) was found in a colony of the brown planthopper, n. lugens, an important plant virus vector. unlike with usual phytopathogenic reoviruses, there are no visible symptoms on a rice plant which has been attacked by nlrv-infected planthoppers. manner of transmission, host range, and multiplication of nlrv in rice plant were studied by enzyme-linked immunosorbent assay or polymerase chain reaction using reverse transcriptase. nlrv was transovumly transmitted to about ... | 1995 | 7871743 |
| nucleotide sequence of nilaparvata lugens reovirus genome segment s8 coding for the major outer capsid protein. | the complete nucleotide sequence of genome segment 8 (s8) of nilaparvata lugens reovirus (nlrv) was determined. it consisted of 1802 nucleotides containing a long open reading frame (562 amino acids), which was expressed in escherichia coli as a fusion protein. the expressed s8 product, a 62k protein, was detected by western blotting using igg directed against intact nlrv particles. this result indicates that s8 encodes the major outer capsid protein of nlrv. the protein exhibited 18.6% amino ac ... | 1994 | 7931170 |
| cloning of the nilaparvata lugens reovirus genome: conserved terminal nucleotide sequences and nucleotide sequence of genome segment s10. | the segmented double-stranded rna genome of nilaparvata lugens reovirus (nlrv) was cloned, and the nucleotide sequence of genome segment s10 and terminal nucleotide sequences of the rest of the segments were determined. genome segment s10 of nlrv consisted of 1430 nucleotides with a single open reading frame extending for 1293 nucleotides from nucleotide 46. it encoded a polypeptide of 431 amino acids with an m(r) of 49.4k, which was a non-structural protein. the plus-strand rna of all genome se ... | 1994 | 8113731 |
| complete nucleotide sequence of the nilaparvata lugens reovirus: a putative member of the genus fijivirus. | the nucleotide sequences of all genome segments of the nilaparvata lugens reovirus (nlrv), which is found in the brown planthopper nilaparvata lugens, have been determined and some genes have been assigned to structural and functional proteins. the genome of nlrv consists of 28 699 nucleotides and contains at least 11 large open reading frames (orfs). the genome of nlrv is the largest among viruses of the family reoviridae reported to date. the deduced amino acid sequence of genome segment s1 co ... | 1996 | 8558122 |
| detection and molecular analysis of plant- and insect-associated bacteria harboring aconitate isomerase involved in biosynthesis of trans-aconitic acid as antifeedant in brown planthoppers. | the activity of aconitate isomerase, which is involved in the biosynthesis of trans-aconitic acid as antifeedant in brown planthoppers, was detected in pseudomonas fluorescens lrb3w1 and pseudomonas putida maff301685 but not in pseudomonas putida maff301684. the enzyme activity was induced in the presence of trans-aconitate, and therefore bacteria showing the enzyme activity were easily detected by their ability to grow on the minimal medium containing trans-aconitate as the sole carbon source ( ... | 1997 | 9216883 |
| alien introgression in rice. | rice (oryza sativa l.) productivity is affected by several biotic and abiotic stresses. the genetic variability for some of these stresses is limited in the cultivated rice germplasm. moreover, changes in insect biotypes and disease races are a continuing threat to increased rice production. there is thus an urgent need to broaden the rice gene pool by introgressing genes for such traits from diverse sources. the wild species of oryza representing aa, bb, cc, bbcc, ccdd, ee, ff, gg and hhjj geno ... | 1997 | 9291958 |
| production and purification of active snowdrop lectin in escherichia coli. | recombinant snowdrop lectin was produced in escherichia coli from a cdna clone encoding mature galanthus nivalis agglutinin. after induction with isopropylthio-beta-d-galactoside, inclusion bodies from e. coli were solubilised and the g. nivalis agglutinin purified by metal-affinity chromatography using a carboxy-terminal hexahistidine tag. the protein was refolded on the metal-affinity column prior to elution. after purification, the recombinant g. nivalis agglutinin agglutinated rabbit erythro ... | 1998 | 9523712 |
| allozyme polymorphism and geographic variation in the small brown planthopper, laodelphax striatellus (homoptera: delphacidae). | the small brown planthopper, laodelphax striatellus, immigrates annually into japan over the east china sea from the asian mainland. it is not known whether this long-distance dispersal has any effect on the genetic structure of japanese l. striatellus populations. the dispersal of l. striatellus is suspected to be relevant to the population dynamics of infection with the parasitic bacterium wolbachia, which causes cytoplasmic incompatibility in l. striatellus. wolbachia infection has spread wit ... | 1997 | 9559095 |
| taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome. | sequence determination of full-length cdna clones of genomic segments 7-10 (s7-s10) of oat sterile dwarf fijivirus (osdv) revealed that the 5' and 3' ends of the plus strands of these segments had the same conserved terminal sequences, 5' aacgaaaaa and uuuuuuuaguc 3'. these sequences are similar, but not identical, to the conserved terminal nucleotide sequences of the genomic segments of rice black streaked dwarf fijivirus (rbsdv) and maize rough dwarf fijivirus (mrdv). the coding strands of s7 ... | 1998 | 9634091 |
| expression of snowdrop lectin (gna) in transgenic rice plants confers resistance to rice brown planthopper. | snowdrop lectin (galanthus nivalis agglutinin; gna) has been shown previously to be toxic towards rice brown planthopper (nilaparvata lugens; bph) when administered in artificial diet. bph feeds by phloem abstraction, and causes 'hopper burn', as well as being an important virus vector. to evaluate the potential of the gna gene to confer resistance towards bph, transgenic rice (oryza sativa l.) plants were produced, containing the gna gene in constructs where its expression was driven by a phloe ... | 1998 | 9753773 |
| rice ragged stunt oryzavirus genome segment s4 could encode an rna dependent rna polymerase and a second protein of unknown function. | the complete nucleotide sequence of genome segment s4 of rice ragged stunt oryzavirus (rrsv, thai-isolate) was determined. the 3823 bp sequence contains two large open reading frames (orfs). orf1, spanning nucleotides 12 to 3776, is capable of encoding a protein of m(r) 141,380 (p4a). the p4a amino acid sequence predicted from the nucleotide sequence contains sequence motifs conserved in rna-dependent rna polymerases (rdrps). when compared for evolutionary relationships with rdrps of other reovi ... | 1998 | 9787664 |
| determining the nucleotide sequence and capsid-coding region of himetobi p virus: a member of a novel group of rna viruses that infect insects. | we determined the complete genome sequence of himetobi p virus (hipv), an insect picorna-like virus, which was isolated from the small brown planthopper, laodelphax striatellus. the genome of hipv consists of 9,275 nucleotides excluding the poly (a) tail, and contains two large open reading frames (orfs), which were separated by a 176-nucleotide noncoding region. the deduced amino acid sequence of the first orf contains core motifs of picornaviral helicase, protease, and rna-dependent rna polyme ... | 1999 | 10550677 |
| taxonomic implications for fijiviruses based on the terminal sequences of fiji disease fijivirus. brief report. | the 5' and 3' terminal sequences of the plus strand of fiji disease fijivirus (fdv) segments 2, 3, 9 and 10 possess the conserved terminal sequences, 5'aaguuuuu.....cagcagauguc 3'. the 5' sequence is identical to that of maize rough dwarf fijivirus (mrdv) and rice black-streaked dwarf fijivirus (rbsdv), whereas the fdv 3' sequence shares the consensus, cagcnnnnguc, with mrdv and rbsdv. the fdv terminal sequences, and the amino acid sequences from fdv segment 9, are more closely related to those ... | 1999 | 10603181 |
| comparison of nucleotide sequences between northern and southern philippine isolates of rice grassy stunt virus indicates occurrence of natural genetic reassortment. | rice grassy stunt virus is a member of the genus tenuivirus, is persistently transmitted by a brown planthopper, and has occurred in rice plants in south, southeast, and east asia [corrected]. we determined the complete nucleotide (nt) sequences of rnas 1 (9760 nt), 2 (4069 nt), 3 (3127 nt), 4 (2909 nt), 5 (2704 nt), and 6 (2590 nt) of a southern philippine isolate from south cotabato and compared them with those of a northern philippine isolate from laguna (toriyama et al., 1997, 1998). the num ... | 2000 | 10612657 |
| cloning, sequence analysis and expression in escherichia coli of the gene encoding a uricase from the yeast-like symbiont of the brown planthopper, nilaparvata lugens. | a urate oxidase (uricase; ec 1.7.3.3) gene of the yeast-like fungal endosymbiont of the brown planthopper, nilaparvata lugens, was cloned, and sequenced together with its flanking regions. the gene comprised a open reading frame of 987 bp, that was split into two parts by a single 96 bp intron. the encoded uricase was 296 amino acids with 62% sequence identity with that of aspergillus flavus. the molecular weight deduced was 32,882, and the predicted isoelectric point was 6.06. the symbiont's ur ... | 2000 | 10696593 |
| ferritin acts as the most abundant binding protein for snowdrop lectin in the midgut of rice brown planthoppers (nilaparvata lugens). | the mannose-specific snowdrop lectin [galanthus nivalis agglutinin (gna)] displays toxicity to the rice brown planthopper nilaparvata lugens. a 26kda gna-binding polypeptide from n. lugens midgut was identified by lectin blotting and affinity chromatography, and characterized by n-terminal sequencing. this polypeptide is the most abundant binding protein for gna in the n. lugens midgut. a cdna (fersub2) encoding this protein was isolated from an n. lugens cdna library. the deduced amino acid seq ... | 2000 | 10727896 |
| differential glycosylation produces heterogeneity in elevated esterases associated with insecticide resistance in the brown planthopper nilaparvata lugens stål. | the major insecticide resistance mechanism in the brown planthopper nilaparvata lugens involves overproduction of esterases. esterases purified from a resistant strain appeared as a ladder of bands on isoelectric focussing (ief) gels from pi 4.7 to 5.0. two-dimensional electrophoresis showed that isozymes ranged in size from 66 to 68 kda with those of lower pi being apparently smaller. all isozymes detected by two-dimensional electrophoresis were glycosylated. n-glycosidase a reduced the number ... | 2000 | 10802235 |
| [phylogenetic relationships and evolution of yeast-like symbionts of the small brown planthoppers based on partial 18s rdna sequences]. | the yeast-like symbionts are isolated and purified from the small brown planthoppers collected from five areas in china. the 18s rdna sequences of them are determined. combined with the sequences of other fungi, we have constructed a comprehensive phylogenetic tree. the results suggested that the taxonomic positions of the yls are in the class pyrenomycetes in the subphylum ascomycotina and the yls have the closest relationship with h. chrysospermus. the yls of different areas in china and japan ... | 2000 | 10887693 |
| photosynthesis and translocation of assimilates in rice plants following phloem feeding by the planthopper nilaparvata lugens (homoptera: delphacidae). | experiments were conducted to measure the effect of feeding by the planthopper nilaparvata lugens (stål) on photosynthesis and the translocation of assimilates in rice plants, oryza sativa l. we used mature japonica rice plants and applied the 13co2 feeding method to evaluate those physiological effects. the photosynthetic rate was suppressed by n. lugens infestation, especially at the lower leaf position, with rates 30% lower than that of control plants at the booting stage. leaf nitrogen conce ... | 2000 | 10985030 |
| molecular characterization of the amplified carboxylesterase gene associated with organophosphorus insecticide resistance in the brown planthopper, nilaparvata lugens. | widespread resistance to organophosphorus insecticides (ops) in nilaparvata lugens is associated with elevation of carboxylesterase activity. a cdna encoding a carboxylesterase, nl-est1, has been isolated from an op-resistant sri lankan strain of n. lugens. the full-length cdna codes for a 547-amino acid protein with high homology to other esterases/lipases. nl-est1 has an n-terminal hydrophobic signal peptide sequence of 24 amino acids which suggests that the mature protein is secreted from cel ... | 2000 | 11122474 |
| comparison of esterase gene amplification, gene expression and esterase activity in insecticide susceptible and resistant strains of the brown planthopper, nilaparvata lugens (stål). | organophosphorus and carbamate insecticide resistance in nilaparvata lugens is based on amplification of a carboxylesterase gene, nl-est1. an identical gene occurs in susceptible insects. quantitative real-time pcr was used to demonstrate that nl-est1 is amplified 3-7-fold in the genome of resistant compared to susceptible planthoppers. expression levels were similar to amplification levels, with 1-15-fold more nl-est1 mrna in individual insects and 5-11-fold more nl-est1 mrna in mass whole body ... | 2000 | 11122475 |
| molecular characterization of bombyx mori cytoplasmic polyhedrosis virus genome segment 4. | the complete nucleotide sequence of the genome segment 4 (s4) of bombyx mori cytoplasmic polyhedrosis virus (bmcpv) was determined. the 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kda. western blot analysis showed that s4 encodes bmcpv protein vp3, which is one of the outer components of the bmcpv virion. sequence analysis of the deduced amino acid sequence of ... | 2001 | 11134312 |
| probing stimulants from the rice plant towards the smaller brown planthopper, laodelphax striatellus (fallen) (homoptera: delphacidae). | when adult males of the smaller brown planthopper, laodelphax striatellus were fed on 2% crude rice (leaf and stem) extract containing 15% sucrose there were characteristic stylet sheaths deposited on parafilm membrane. further bioassays with the butanol-soluble fr. of the extract revealed that it is highly effective for the insects. when the butanol fr. was charged on an ods open column and eluted in sequence with 20, 40 and 100% methanol in water, the ods-40% methanol fr. was shown as the most ... | 2015 | 11204184 |
| characterization of euploid backcross progenies derived from interspecific hybrids between oryza sativa and o. eichingeri by restriction fragment length polymorphism (rflp) analysis and genomic in situ hybridization (gish). | restriction fragment length polymorphism (rflp) analysis and gish (genomic in situ hybridization) were performed on euploid plants derived from crosses between oryza sativa (2n = 24, aa) and two brown planthopper-resistant accessions of o. eichingeri (2n = 24, cc). after screening with 164 rflp markers, 60 of the 67 euploid plants were identified as introgression lines, each carrying 1-6 small o. eichingeri segments integrated on chromosomes 1, 2, 6, or 10. in the somatic chromosome preparations ... | 2001 | 11269361 |
| insect symbiosis: derivation of yeast-like endosymbionts within an entomopathogenic filamentous lineage. | yeast-like endosymbionts (ylss) of insects often are restricted to specific hosts and are essential to the host's survival. for example, in planthoppers (homoptera: delphacidae), endosymbionts function in sterol utilization and nitrogen recycling for the hosts. our study, designed to investigate evolutionary changes in the yls lineage involved in the planthopper association, strongly suggests an origin of the ylss from within the filamentous ascomycetes (euascomycetes), not the true yeasts (sacc ... | 2001 | 11371588 |
| glutathione s-transferases as antioxidant defence agents confer pyrethroid resistance in nilaparvata lugens. | selection of a laboratory colony of the brown planthopper nilaparvata lugens with the pyrethroids permethrin and lambda-cyhalothrin increased its resistance to both insecticides. biochemical analysis and synergistic studies with metabolic inhibitors indicated that elevated glutathione s-transferases (gsts) with a predominant peroxidase activity conferred resistance to both pyrethroids, whereas esterases conferred part of the resistance to permethrin. purified esterases hydrolysed permethrin at a ... | 2001 | 11415437 |
| histological observations on transovarial transmission of a yeast-like symbiote in nilaparvata lugens stal (homoptera, delphacidae). | transovarial transmission of a yeast-like symbiote (yls) in the brown planthopper, nilaparvata lugens stal, was observed with light and electron microscopy. light micrographs showed that there was no yls in testes and spermathecae of the mated females, indicating that sperm is not involved in the transovarial transmission of the symbiote. both light and electron micrographs showed the processes of yls transmission from fat body to the oocyte. in females, the symbiotes in mycetocytes moved out of ... | 2001 | 11469541 |
| spectral characteristics of rice plants infested by brown planthoppers. | spectral characteristics of rice plants at various levels of infestation by the brown planthopper, nilaparvata lugens (stål), (homoptera:delphacidae), in the early grain-filling stage were measured and analyzed using a spectroradiometer. plant damage was classified into six scales, i.e., 0 (ck), 1, 3, 5, 7 and 9, based on the scale of infestation displayed on the surfaces of plant parts. results showed that mean curves of reflectance spectra (350 - 1800 nm) from different scales of insect infest ... | 2001 | 11480774 |
| [spatial structure and pattern of nilaparvata lugens population in large-scale]. | brown planthopper(bph), nilaparvata lugens, is a major pest of rice. its spatial distribution of different generations in guangdong province was studied by using variograms. the results showed that this population distributed in a clump pattern in rice fields during the 1st rice season. 3 generations of this population had different spatial structures, with the clump range of 400 km, 200 km and 205 km respectively in the 1st rice season. the simulation distribution maps of bph, which was interpo ... | 2001 | 11757373 |
| [stress response of nilaparvata lugens at high temperature and activities of its protective enzyme systems]. | the nymphae and different wing-form adults of brown planthopper (bph) were treated intermittently at 34 degrees c, 36 degrees c and 38 degrees c. during the process of heat shock treatment, the variation of the activities of the endogenous enzymes of protective system and the content of lipid peroxides (lpo) in bph were researched. the results showed that the effect of heat shock treatments on cat activity in 4th instar of nymphae was higher than that in 3rd and 5th instar. the cat activity in b ... | 2001 | 11758425 |
| [tolerance of various geographic populations of brown planthopper to adverse environmental stresses]. | the populations of brown planthopper(bph), nilaparvata lugens, were collected from zhejiang, guangxi and yunnan provinces to study their tolerance to various adverse environmental stresses. the results indicated that all of these three populations could adapt to the rice variety ir26 bearing resistant gene 1 (bph1), and guangxi and yunnan populations could successfully infest rice varieties asd7 bearing bph2 and rathu heenati bearing bph3, implying that these two populations had a higher adaptab ... | 2000 | 11767535 |
| [effect of rice variety resistance on population dynamics of nilaparvata lugens and sogatella furcifera]. | the effect of rice varieties with different insect-resistance on dynamics of nilaparvata lugens and sogatella furcifera population was examined, based on the investigation of their inhabiting ratio, egg amount, survival rate of egg and nymph, nymph development, and the amount of their offspring population. four rice varieties, tn1, n22, asd7 and ptb33 were investigated. ptb33 had a significantly lower inhabiting ratio, egg amount, and survival ratio of eggs and nymphs of both parasitic populatio ... | 2000 | 11767559 |
| molecular characterisation of segments 1 to 6 of rice black-streaked dwarf virus from china provides the complete genome. | the nucleotide sequences of segments s1 to s6 of a chinese isolate of rice black-streaked dwarf virus (rbsdv) were determined. this provides the first complete sequence of a plant pathogenic member of the genus fijivirus. the complete ten-segment genome has 29,141 nucleotides, making it the largest reovirus genome so far reported. each of the segments s1-s6 is predicted to encode a single major protein. protein comparisons indicated that s1 encoded an rna dependent rna polymerase, with similarit ... | 2001 | 11811683 |
| purification, molecular cloning and heterologous expression of a glutathione s-transferase involved in insecticide resistance from the rice brown planthopper, nilaparvata lugens. | a novel glutathione s-transferase (gst)-based pyrethroid resistance mechanism was recently identified in nilaparvata lugens [vontas, small and hemingway (2001) biochem. j. 357, 65-72]. to determine the nature of gsts involved in conferring this resistance, the gsts from resistant and susceptible strains of n. lugens were partially purified by anion exchange and affinity chromatography. the majority of peroxidase activity, previously correlated with resistance, was confined to the fraction that b ... | 2002 | 11853540 |
| [detection and analysis of qtl for resistance to the brown planthopper, nilaparvata lugens (stål), in rice (oryza sativa l.), using backcross inbred lines]. | a mapping population of 98 bc1f9 lines (backcross inbred lines: bils), derived from a backcross of nipponbare (japonica)/kasalath (indica)//nipponbare by the single-seed descent methods, was used to detect quantitative trait loci (qtl) for resistance to brown planthopper (bph), nilaparvata lugens (stål). seedbox screening technique (sst) was applied to evaluate the reactions of two parents and 98 bils to bph at the seedling stage, and the entries were graded on ratio of dead seedlings. a total o ... | 2002 | 11985267 |
| electroantennogram responses of nilaparvata lugens (homoptera: delphacidae) to plant volatile compounds. | electroantennogram (eag) recordings were made from the plaque organ receptors of the rice brown plant hopper, nilaparvata lugens (stål). the receptors of the distal plaque organ of both male and female hoppers responded to the air from above chopped rice plants, and those of females gave a larger response. female hoppers, tested with 27 plant volatiles, gave dose-related responses to 16 of the compounds, including a range of green leaf volatiles. the aliphatic aldehydes hexanal and (e)-2-hexenal ... | 2002 | 12020000 |
| localization of ps9 in rice ragged stunt oryzavirus and its role in virus transmission by brown planthopper. | a cdna of rice ragged stunt oryzavirus(rrsv) coding for its protein ps9 was prepared and expressed in e.coli as a fusion protein then purified and cleaved by factor-xa to a 38kd polypeptide. using the igg of the antiserum raised against the expressed fusion protein the gold immuno-labelling experiments provided a direct evidence that the 38kd polypeptide is one of the major proteins forming the virus spikes. virus transmission experiments in brown planthopper fed with ps9 showed the inhibition o ... | 1999 | 12136212 |
| molecular mapping and genetic analysis of a rice brown planthopper (nilaparvata lugens stål) resistance gene. | the brown planthopper (bph), nilaparvata lugens stål, is a serious insect pest of rice (oryza saliva l.). we have determined the chromosomal location of a bph resistance gene in rice using ssr and rflp techniques. a rice line 'b14', derived from the wild rice oryza latifolia, showed high resistance to bph. for tagging the resistance gene in 'b14x', an f2 population and a recombinant inbred (ri) population from a cross between taichung native 1 and 'b14' were developed and evaluated for bph resis ... | 2002 | 12184487 |
| nucleotide sequences of double-stranded rna segments from a hypovirulent strain of the white root rot fungus rosellinia necatrix: possibility of the first member of the reoviridae from fungus. | twelve double-stranded (ds) rna segments were detected from a hypovirulent strain w370 of the white root rot fungus rosellinia necatrix. the estimated molecular weights ranged from 0.41 x 10(6) to 2.95 x 10(6). full length cdna clones for eight segments were obtained. northern blot analysis suggested that each segment was genetically unique. the nucleotide sequences of eight full length dsrna segments were determined. one long open reading frame was found in each segment. conserved sequences at ... | 2002 | 12206302 |
| sequence analysis of genome segments s4 and s8 of mal de río cuarto virus (mrcv): evidence that the virus should be a separate fijivirus species. | this is the first sequence-based characterization of mal de río cuarto virus (mrcv), currently classified as a variant of maize rough dwarf virus (mrdv) and exclusively found in south america. we sequenced and analyzed genome segments s4 and s8. mrcv s4 coded for a putative 131.67 kda protein while mrcv s8 coded for a putative 68.26 kda protein containing an atp/gtp-binding motif. the 5' and 3' ends of mrcv segments, were 5'aaguuuuu3' and 5'cagcunnnguc3', respectively. prediction of secondary st ... | 2002 | 12209310 |
| putative protein digestion in a sap-sucking homopteran plant pest (rice brown plant hopper; nilaparvata lugens: delphacidae)--identification of trypsin-like and cathepsin b-like proteases. | sap-sucking phytophagous insect species of the order hemiptera have been assumed not to carry out digestive proteolysis, but instead to rely on free amino acids in the phloem and xylem saps for their nutritional requirements. extracts prepared from isolated guts of rice brown planthopper (nilaparvata lugens), a homopteran crop pest, were shown to contain protease activity, with hydrolysis of both protein and synthetic peptide substrates being observed. assays with specific inhibitors suggested t ... | 2002 | 12213233 |
| the role of ants, especially the fire ant, solenopsis geminata (hymenoptera: formicidae), in the biological control of tropical upland rice pests. | predatory ants are omnipresent year-round in upland (dryland) rice fields in the philippines. at least 14 species were identified of which the very aggressive solenopsis geminata (fabricius) and also tapinoma sp. nr indicum forel usually predominated. some highly aggressive species, notably pheidolegetonspp. and bothriomyrmex dalyi forel were localized. solenopsis geminata flourished within many fields, not only during the crop season but also throughout dry season fallows where they remained ag ... | 2002 | 12241568 |
| detection of rice grassy stunt tenuivirus nonstructural proteins p2, p5 and p6 from infected rice plants and from viruliferous brown planthoppers. | the genome of rice grassy stunt virus (rgsv) consists of 6 ambisense rna segments, among which rnas 1, 2, 5 and 6 are equivalent to rnas 1, 2, 3 and 4, respectively, of rice stripe virus, the type species of the genus tenuivirus. the rgsv 36-kda nucleocapsid protein (n) is encoded on the complementary strand of rna 5. here, we studied accumulation of three nonstructural proteins, a 23-kda p2 protein encoded on vrna 2 (virus genomic strand), a 22-kda p5 protein encoded on vrna 5, and a 21-kda p6 ... | 2002 | 12491098 |
| synthesis and insecticidal activity of n-oxydihydropyrroles: 4-hydroxy-3-mesityl-5,5-dimethyl derivatives with various substituents at the 1-position. | a new series of n-oxydihydropyrrole derivatives was synthesized and evaluated for insecticidal activity against nilaparvata lugens and myzus persicae. various substituents were introduced to the 1-position of the dihydropyrrole ring, and the derivatives obtained exhibited systemic and/or contact insecticidal activity. the structure-activity relationship revealed that small alkyoxy and alkoxyalkoxy groups were more favorable than alkylcarbonyloxy, alkoxycarbonyloxy, or sulfonyloxy groups as subst ... | 2002 | 12506980 |
| superinfection of laodelphax striatellus with wolbachia from drosophila simulans. | wolbachia are maternally inherited, intracellular alpha-proteobacteria that infect a wide range of arthropods. they manipulate the reproduction of hosts to facilitate their spread into host populations, through ways such as cytoplasmic incompatibility (ci), parthenogenesis, feminization and male killing. the influence of wolbachia infection on host populations has attracted considerable interest in their possible role in speciation and as a potential agent of biological control. in this study, w ... | 2003 | 12522428 |
| in vitro cultivation of wolbachia in insect and mammalian cell lines. | wolbachia infecting the small brown planthopper, laodelphax striatellus, were successfully maintained and cultivated in two insect and one mammalian cell lines. the bacteria with the planthopper ovary were introduced into the flasks with the cultures of the cell lines. the wolbachia proliferated in mosquito (aedes albopictus) and lepidopteran (heliothis zea) cell lines and in the mouse cell line, l929. proliferation of wolbachia was confirmed by electron microscopy and quantitative polymerase ch ... | 2002 | 12534342 |
| synthesis and insecticidal activity of novel dihydropyrrole derivatives with n-sulfanyl, sulfinyl, and sulfonyl moieties. | this paper reports the synthesis and insecticidal activity of a new type of dihydropyrrole derivatives with sulfur moieties such as sulfanyl, sulfinyl, and sulfonyl groups at the 1-position. these derivatives exhibited high insecticidal potency against nilaparvata lugens and nephotettix cincticeps. investigation of the structure-activity relationships revealed that the alkoxycarbonyloxy groups at the 4-position tended to increase the systemic insecticidal activity. | 2003 | 12538013 |
| rapd markers linked to brown planthopper nilaparvatha lugens resistance locus in rice. | brown plant hopper, a major pest in rice causes "hopper burn" in the field. the resistance gene for brown planthopper was mapped by using 20 recombinant inbred lines (ril's) derived from a cross between resistant line oryza. officinalis derivative (ir 54742-2-21-12-17-6) and a susceptible rice cultivar asd 16 using bulked segregant analysis. on an average of 4 loci were amplified and two rapd primers amplified loci that co-segregated with resistance/susceptibility. the segregating rapd loci were ... | 2000 | 12561959 |
| a genome-specific repetitive dna sequence from oryza eichingeri: characterization, localization, and introgression to o. sativa. | in the course of transferring the brown planthopper resistance from a diploid, cc-genome wild rice species, oryza eichingeri (irgc acc. 105159 and 105163), to the cultivated rice variety 02428, we have isolated many alien addition and introgression lines. the o. eichingeri chromatin in some of these lines has previously been identified using genomic in situ hybridization and molecular-marker analysis. here we cloned a tandemly repetitive dna sequence from o. eichingeri irgc acc105163, and detect ... | 2002 | 12582684 |
| rflp-facilitated investigation of the quantitative resistance of rice to brown planthopper ( nilaparvata lugens). | quantitative trait loci (qtls), conferring quantitative resistance to rice brown planthopper (bph), were investigated using 160 f(11) recombinant inbred lines (rils) from the lemont/teqing cross, a complete rflp map, and replicated phenotyping of seedbox inoculation. the paternal indica parent, teqing, was more-resistant to bph than the maternal japonica parent, lemont. the rils showed transgressive segregation for resistance to bph. seven main-effect qtls and many epistatic qtl pairs were ident ... | 2002 | 12582694 |
| sequence and phylogenetic analysis of genome segments s1, s2, s3 and s6 of mal de río cuarto virus, a newly accepted fijivirus species. | mal de río cuarto virus (mrcv) is a newly described species of the genus fijivirus, family reoviridae. the nucleotide sequence of four mrcv genome segments was determined. mrcv s1, s2, s3 and s6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kda, respectively. mrcv s1 encodes a basic protein that contains conserved rna-dependent rna polymerase motifs, and is homologous to rice black streaked dwarf virus (rbsdv), fiji disease virus (fdv) and nilaparvata lugens reovirus (nlrv) pol ... | 2003 | 12606083 |
| [application of latent root regression method in analysis of predation of predatory natural enemy in paddy field]. | a latent root regression model was constructed for analyzing the predation quantity in a coexistence system of four spiders (pirata subpiraticus, oedothorax insecticeps, clubiona japonicola, and bianor hotingchiehi) with brown planthopper (nilaparvata lugens). the predation quantity of the spiders in a multi-species coexistence system could be predicted by y = 6.6600 +/- 6.3399(- 0.0138x1* + 0.0895x2. + 0.0078x3* - 0.1067x4* + 0.6742x5*). the result showed that compared to ls regression, the acc ... | 2002 | 12682962 |
| alien genes introgression and development of monosomic alien addition lines from oryza latifolia desv. to rice, oryza sativa l. | oryza latifolia, a tetraploid wild relative of cultivated rice is an important source of resistance to bacterial blight (bb), the brown planthopper (bph) and the whitebacked planthopper (wbph). interspecific hybrids were obtained between an elite breeding line (ir31917-45-3-2) of oryza sativa (2n=24 aa) and o. latifolia acc. no. 100914 (2n=48 ccdd). the crossability in f1 was 7.58% and it ranged from 0.11 to 0.62 in backcross generations. the f1 hybrid showed 2-6 ii, 0-2 iii, 0-1 iv and 22-32 i; ... | 2003 | 12764581 |
| immunohistochemical and developmental studies to elucidate the mechanism of action of the snowdrop lectin on the rice brown planthopper, nilaparvata lugens (stal). | rice brown planthoppers (nilaparvata lugens) were fed on artificial diet containing snowdrop lectin (galanthus nivalis agglutinin; gna), which has been shown to be toxic towards this insect pest. in addition to decreasing survival, the lectin affected development, reducing the growth rate of nymphs by approximately 50% when present at a concentration of 5.3&mgr;m. immunolocalisation studies showed that lectin binding was concentrated on the luminal surface of the midgut epithelial cells within t ... | 1998 | 12769935 |
| sensitive periods for wing development and precocious metamorphosis after precocene treatment of the brown planthopper, nilaparvata lugens. | the critical periods for juvenile hormone suppression of wing development and metamorphosis were examined in a pure brachypterous line of nilaparvata lugens following topical application of precocene ii (pii) to various stages of the third and fourth nymphal stadia. when pii, in doses ranging from 10 pg to 100 ng, was applied to 12-h-old third or 6-h-old fourth stadium nymphs, long-wing formation (macroptery) was induced. macropter induction ranged from 5 to 50% in females and from 30 to 50% in ... | 2002 | 12770122 |
| resistance to green leafhopper (nephotettix virescens) and brown planthopper (nilaparvata lugens) in transgenic rice expressing snowdrop lectin (galanthus nivalis agglutinin; gna). | transgenic rice plants expressing snowdrop lectin (galanthus nivalis agglutinin; gna) were screened for resistance to green leafhopper (nephotettix virescens; glh), a major homopteran pest of rice. survival was reduced by 29% and 53% (p<0.05) respectively, on plants where gna expression was tissue-specific (phloem and epidermal layer) or constitutive. similar levels of resistance in gna-expressing transgenic rice were previously reported for rice brown planthopper (nilaparvata lugens; bph). gna ... | 2000 | 12770221 |
| enhancement of short wing formation and ovarian growth in the genetically defined macropterous strain of the brown planthopper, nilaparvata lugens. | when jh ii, iii or methoprene was applied in the nymphal stages to two different strains of the brown planthopper which were selected to produce long (macropterous) or short (brachypterous) wing forms, no effect was observed on the molting profile or metamorphosis. brachypterization of a majority of the presumptive macropters was, however, observed by application of these chemicals, although there was no effect on wing form in the presumptive brachypters. the results show that the sensitive peri ... | 1999 | 12770400 |
| detecting rice stripe virus (rsv) in the small brown planthopper (laodelphax striatellus) with high specificity by rt-pcr. | rice stripe disease, caused by rice stripe virus (rsv), may lead to severe or even crippling losses in many rice-cultured countries and regions. as the most important vector of rsv, the small brown planthopper (sbph) (laodelphax striatellus) is largely responsible for the epidemic phase of the disease. therefore, a rapid identification of rsv in the sbph is of a great need for disease forecasting. a reverse transcription polymerase chain reaction (rt-pcr) assay is described to amplify a rsv gene ... | 2003 | 12951219 |
| sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. | the complete nucleotide sequences of 10 genomic segments (s1-s10) from an isolate of rice black-streaked dwarf virus causing rough dwarf disease on maize (rbsdv-hbm) in china were determined, a total of 29,142 base pairs (bp). each segment possessed the genus-specific termini with conserved nucleotide sequences of (+) 5'-aaguuuuu......cagcunnnguc-3' and a perfect or imperfect inverted repeat of seven to eleven nucleotides immediately adjacent to the terminal conserved sequence. while the coding ... | 2003 | 14501194 |
| construction of a genomic library of wild rice and agrobacterium-mediated transformation of large insert dna linked to bph resistance locus. | here we report the first genomic library of wild rice constructed on a plant-transformation-competent binary vector (bibac2) and transformation of the large insert dna into rice via agrobacterium. we selected oryza officinalis for genomic library construction. the library consists of 55,296 clones and stored in one hundred forty-four 384-well plates. random sampling of 140 clones indicated an average insert size of 71 kb at a range of 15-235 kb and 4.8% empty vectors. four wheat chloroplast prob ... | 2003 | 14636998 |
| [chaotic diagnosis of nilaparvata lugens occurrence system]. | it is estimated that there are nearly 20 million hectares of rice crop being infested by nilaparvata lugens, with an annual loss of some half a million tones of grain. in order to study the properties and laws of nilaparvata lugens occurrence system further, the time series data of nilaparvata lugens occurrence from 1986 to 1998 collected in taihu lake district were analyzed by using the methods of autocorrelation function, power spectrum figure, phase trajectory figure, poincare section, and re ... | 2003 | 14655376 |
| two whitebacked planthopper resistance genes in rice share the same loci with those for brown planthopper resistance. | the whitebacked planthopper (wbph), sogatella furcifera, and brown planthopper (bph) nilaparvata lugens stål are important sucking insects of rice (oryza sativa l.) crops throughout the world. rice 'b5', which has derived its resistance genes from the wild rice o. officinalis wall ex watt, is a line that is highly resistant to both wbph and bph. previously, two resistance genes against bph, qbp1, and qbp2 in 'b5' had been mapped onto chromosome 3 and chromosome 4, respectively. in this study, we ... | 2004 | 14666132 |
| selection for imidacloprid resistance in nilaparvata lugens: cross-resistance patterns and possible mechanisms. | a field population of brown planthoppers (nilaparvata lugens stål) was collected and selected for imidacloprid resistance in the laboratory. the resistance increased by 11.35 times in 25 generations and the resistance ratio reached 72.83 compared with a laboratory susceptible strain. the selected resistant strain showed obvious cross-resistance to all the acetylcholine receptor targeting insecticides tested (monosultap 1.44-fold, acetamiprid 1.61-fold, imidacloprid homologues js599 2.46-fold and ... | 2003 | 14667058 |
| nutrient uptake of rice roots in response to infestation of nilaparvata lugens (stål) (homoptera: delphacidae). | this study investigated the uptake of three macronutrients, including nitrogen (n), phosphorus (p), and potassium (k), by rice roots in response to different infestation levels of nilaparvata lugens (stål) (homoptera: delphacidae). hydroponics experiments were conducted on the rice variety 'zhendao 2' (moderately resistant to tryporyza incertulas, lepidoptera: pyralidae) and the variety 'xiushui 63' (susceptible to n. lugens). in 'zhendao 2', n. lugens infestation significantly influenced the up ... | 2003 | 14977118 |
| do rice hybrids have heterosis for insect resistance? a study with nilaparvata lugens (hemiptera: delphacidae) and marasmia patnalis (lepidoptera: pyralidae). | antibiosis-based resistance to two insect pests of rice, nilaparvata lugens (stål) (hemiptera: delphacidae) and marasmia patnalis bradley (lepidoptera: pyralidae), was compared in 11 f1 hybrids and their parental lines. our objective was to determine whether hybrids show heterosis (hybrid vigor) for insect resistance or susceptibility. heterosis is defined as the amount by which a hybrid exceeds its midparent value or its better parent. overall, we did not find evidence of heterosis or heterobel ... | 2003 | 14977136 |
| differential gene expression in response to brown planthopper feeding in rice. | plant responses to herbivores are complex. 108 cdna clones representing genes relating to plant responses to chewing insect-feeding, pathogen infection, wounding and other stresses were collected. northern blot and cdna array analysis were employed to investigate gene expression regulated by piercing-sucking insect, brown planthopper (bph), nilaparvata lugens (homoptera: dephacidae) on both the resistant and susceptible rice genotypes. after bph feeding in rice for 72 h, the expression of most t ... | 2004 | 15002664 |
| isolation and characterization of a novel rice gene encoding a putative insect-inducible protein homologous to wheat wir1. | a full-length cdna, designated bphi008a, was cloned representing a rice (oryza sativa) mrna that accumulates after brown planthopper (bph) nilapar vata lugens stål feeding. the cdna encodes a putative 82 amino acid protein (bphi008a) exhibiting about 37% amino acid sequence identity to wir1 family of proteins that are encoded by pathogen-induced transcripts in wheat. like wir1 proteins, it consists of a hydrophobic n-terminal half and a hydrophilic c-terminal half relatively rich in glycine and ... | 2004 | 15002667 |
| marker-assisted pyramiding of brown planthopper (nilaparvata lugens stål) resistance genes bph1 and bph2 on rice chromosome 12. | brown planthopper (bph) (nilaparvata lugens stål) is a significant insect pest of rice (oryza sativa l.). we constructed a gene-pyramided japonica line, in which two bph resistance genes bph1 and bph2 on the long arm of chromosome 12 independently derived from two indica resistance lines were combined through the recombinant selection. the gene-pyramiding was achieved based on the previously constructed high-resolution linkage maps of the two genes. two co-dominant and four dominant pcr-based ma ... | 2004 | 15032948 |
| proteomic analysis of brown planthopper: application to the study of carbamate toxicity. | toxicity to o-sec-butylphenyl methylcarbamate compound (bpmc) was analyzed in the rice brown planthopper, nilaparvata lugens, using a differential proteomics approach of identifying proteins on two dimensional-polyacrylamide gel electrophoresis (2d-page). proteome analysis from bpmc-treated brown planthopper resulted in the modulation of 22 proteins at the expression level as compared to control samples on coomassie brilliant blue (cbb) stained gels. out of total 22 proteins, 10 proteins showed ... | 2004 | 15110863 |
| [techniques of diseases, insect pests and weeds control and their efficacy in bio-rational rice production]. | studies on the efficacy of bio-rational pesticides and agricultural methods against the chief diseases, insect pests and weeds of rice showed that the efficacy of the mixtures of jingangmycin and bacillus-cereus, and jingangmycin and polyoxin against rice sheath blight were 75.16%-94.27% after sprayed once at the tiller and boot end stages of rice, respectively, and better than that of chemical fungicide triadimefon. the efficacy of kasugamycin and blasticidin was 50.54%-72.67% on rice leaf blas ... | 2004 | 15139200 |
| [qtl analysis for rice stripe disease resistance gene using recombinant inbred lines (rils) derived from crossing of kinmaze and dv85]. | rice stripe disease transmitted by small brown planthopper (laodelphax striatellus fall.) is one of the most serious viral diseases in east asia. the disease is severely epidemic in most rice growing areas where the main cultivars are susceptible or moderately susceptible to rice stripe virus. in this research, a recombinant inbred lines (rils) population of 81 lines derived from a cross of kinmaze (japonica)/dv85(indica) by the single seed descent method was used to detect quantitative trait lo ... | 2004 | 15195569 |
| transgenic rice plants expressing the snowdrop lectin gene (gna) exhibit high-level resistance to the whitebacked planthopper (sogatella furcifera). | transgenic rice plants, expressing snowdrop lectin [galanthus nivalis agglutinin (gna)], obtained by agrobacterium-mediated genetic transformation, were evaluated for resistance against the insect, the whitebacked planthopper (wbph). the transgene gna was driven by the phloem-specific, rice-sucrose synthase promoter rss1, and the bar was driven by the camv 35s promoter. in our previous study, the transgenic status of these lines was confirmed by southern, northern and western blot analyses. both ... | 2004 | 15252708 |
| molecular cloning and immunolocalization of a diuretic hormone receptor in rice brown planthopper (nilaparvata lugens). | rna extracted from guts of rice brown planthopper, nilaparvata lugens, was used to clone cdna predicted to encode a diuretic hormone receptor (dhr). the dhr, a member of the calcitonin/secretin/corticotropin-releasing factor family of g-protein-coupled receptors, contains seven transmembrane domains and a large n-terminal extracellular domain potentially involved in hormone binding. the n-terminal domain was expressed as a recombinant protein, purified and used to raise antibodies. anti-dhr igg ... | 2004 | 15373805 |
| mechanisms of hopperburn: an overview of insect taxonomy, behavior, and physiology. | hopperburn is a non-contagious disease of plants caused by the direct feeding damage of certain leafhoppers and planthoppers. although long studied, especially with empoasca spp. leafhoppers (cicadellidae: typhlocybinae), the mechanisms underlying hopperburn have only recently been elucidated. hopperburn is caused by a dynamic interaction between complex insect feeding stimuli (termed hopperburn initiation) and complex plant responses (termed the hopperburn cascade). herein we review the nature ... | 2005 | 15471532 |
| [mapping of a new resistance gene to bacterial blight in rice line introgressed from oryza officinalis]. | rice line 'b5', which was derived from the wild rice oryza officinalis wall ex watt through introgression, has been proved to be high resistant to brown planthopper, whitebacked planthopper and bacterial blight (xanthomonas oryzae pv. oryzae). in this study, the resistance to bacterial blight of 187 recombinant inbred lines (rils) from a cross between ' b5' and 'minghui63' were evaluated and rflp markers linked to the resistance gene were identified by bulked segregant analysis. analysis of the ... | 2004 | 15473325 |
| high-resolution genetic mapping at the bph15 locus for brown planthopper resistance in rice (oryza sativa l.). | resistance to the brown planthopper (bph), nilaparvata lugens stal, a devastating sucking insect pest of rice, is an important breeding objective in rice improvement programs. bph15, one of the 17 major bph resistance genes so far identified in both cultivated and wild rice, has been identified in an introgression line, b5, and mapped on chromosome 4 flanked by restriction fragment length polymorphism markers c820 and s11182. in order to pave the way for positional cloning of this gene, we have ... | 2004 | 15549231 |
| identification of genes responsive to brown planthopper nilaparvata lugens stal (homoptera: delphacidae) feeding in rice. | the brown planthopper (nilaparvata lugens stal) is a rice (oryza sativa l.)-specific herbivore that ingests assimilates from the phloem, which leads to stunted growth and therefore poor yield of rice. in this study, 27 planthopper-responsive genes in rice plants were isolated by suppression subtractive hybridization (ssh), and confirmed by filter cdna array hybridization and northern blot analysis. among these transcripts, 25 were induced and 2 were suppressed by n. lugens feeding. the responsiv ... | 2005 | 15558307 |
| insect resistance to nilaparvata lugens and cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna+sbti transgenes. | molecular genetic analysis and insect bioassay of transgenic indica rice 'zhuxian b' plants carrying snowdrop lectin gene (gna) and soybean trypsin inhibitor gene (sbti) were investigated in detail. pcr, 'dot' blot and pcr-southern blot analysis showed that both transgenes had been incorporated into the rice genome and transmitted up to r3 progeny in most lines tested. some transgenic lines exhibited mendelian segregation, but the other showed either 1:1 (positive: negative for the transgenes) o ... | 2005 | 15593292 |
| [isolation of rice genes down-regulated by brown planthopper infestation by suppression subtractive hybridization]. | suppression subtractive hybridization (ssh) was used to isolate rice genes down-regulated by bph infestation. the ssh cdna library constructed with the tester cdna from rice seedlings, and driver cdna from rice seedlings fed by bph consists of 200 clones. fifty clones were randomly picked and screened by reverse northern dot blot and northern blot. as a result, two cdna fragments have been isolated: one is lhca, which encodes rice photosystem i antenna protein; the other is a novel rice gene, an ... | 2004 | 15599027 |
| changes of zeatin riboside content in rice plants due to infestation by nilaparvata lugens (homoptera: delphacidae). | the effect of nilaparvata lugens (stål) (homoptera: delphacidae), infestation on the content of zeatin ribosides (zr) in rice plants was investigated with enzyme-linked immunosorbent assay. hydroponics experiments were conducted on 'zhendao 2' rice, in which plants were subjected to n. lugens infestation at three nonhopperburn-causing densities (15, 30, and 60 nymphs per hill) for 2, 4, 6, and 8 d and at one hopperburn-causing density (240 nymphs per hill) for 2, 4, and 6 d, respectively. when r ... | 2004 | 15666745 |
| determination and distribution of a female-specific protein in the brown planthopper, nilaparvata lugens stal (homoptera: delphacidae). | electrophoretic analyses of hemolymph and body or ovary homogenates from reproducing females, males, and 5th instar nymphs of the brown planthopper, nilaparvata lugens stal, revealed a protein band of 175 kda in females. an immunoblot test using antibody against this protein showed a positive reaction with a 175 kda protein from female body or ovary homogenates. it is likely that this protein in hemolymph is vitellogenin (vg). distribution of vg was determined by immunofluorescence and immunogol ... | 2005 | 15695174 |
| [secondary compounds in rice varieties resistant to nilaparvata lugens]. | by the method of high-performance liquid chromatography (hplc), this paper studied the contents of 13 secondary compounds from 130 samples of 26 rice varieties resistant to brown planthopper (bph) nilaparvata lugens biotype ii. a prediction model was established through principal component analysis and multiple regression analysis: y = 3.4593 - 0.02491 x1 + 0.08475 x2 - 0.04227 x8 + 0.1174 x12. the relationships between the bph-resistant level of rice varieties and the area values of chromatogra ... | 2004 | 15707334 |
| time-concentration-mortality modeling of the synergistic interaction of beauveria bassiana and imidacloprid against nilaparvata lugens. | interactions between the entomopathogenic fungus, beauveria bassiana sg8702, and imidacloprid on nilaparvata lugens (homoptera: delphacidae) were studied in laboratory bioassays by spraying suspensions of unformulated conidia (assay 1) and aqueous dilutions of emulsifiable conidia formulation alone (assay 2) or together with imidacloprid at the sub-lethal rates of 0.5, 1.0 and 2.0 microg ml(-1) (assays 3-5). each assay consisted of five conidia concentrations plus an appropriate control and thre ... | 2005 | 15751011 |
| cloning and characterization of a beta3 tubulin cdna from the small brown planthopper, laodelphax striatellus. | 2005 | 15859520 | |
| molecular dynamics of detoxification and toxin-tolerance genes in brown planthopper (nilaparvata lugens stål., homoptera: delphacidae) feeding on resistant rice plants. | to investigate the molecular response of brown planthopper, nilaparvata lugens (bph) to bph-resistant rice plants, we isolated cdna fragments of the genes encoding for carboxylesterase (car), trypsin (try), cytochrome p450 monooxygenase (p450), nadh-quinone oxidoreductase (nqo), acetylcholinesterase (ace), and glutathione s-transferase (gst). expression profiles of the genes were monitored on fourth instar nymphs feeding on rice varieties with different resistance levels. northern blot hybridiza ... | 2005 | 15898115 |
| a nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid in nilaparvata lugens (brown planthopper). | neonicotinoids, such as imidacloprid, are nicotinic acetylcholine receptor (nachr) agonists with potent insecticidal activity. since its introduction in the early 1990s, imidacloprid has become one of the most extensively used insecticides for both crop protection and animal health applications. as with other classes of insecticides, resistance to neonicotinoids is a significant threat and has been identified in several pest species, including the brown planthopper, nilaparvata lugens, a major r ... | 2005 | 15937112 |
| exogenous application of jasmonic acid induces volatile emissions in rice and enhances parasitism of nilaparvata lugens eggs by the parasitoid anagrus nilaparvatae. | jasmonate signaling pathway plays an important role in induced plant defense against herbivores and pathogens, including the emission of volatiles that serve as attractants for natural enemies of herbivores. we studied the volatiles emitted from rice plants that were wounded and treated with jasmonic acid (ja) and their effects on the host-searching behavior of the rice brown planthopper, nilaparvata lugens (stål), and its mymarid egg parasitoid anagrus nilaparvatae pang et wang. female adults o ... | 2005 | 16132208 |
| attraction of the parasitoid anagrus nilaparvatae to rice volatiles induced by the rice brown planthopper nilaparvata lugens. | anagrus nilaparvatae, an egg parasitoid of the rice brown planthopper nilaparvata lugens, was attracted to volatiles released from n. lugens-infested plants, whereas there was no attraction to volatiles from undamaged plants, artificially damaged plants, or volatiles from n. lugens nymphs, female adults, eggs, honeydew, and exuvia. there was no difference in attractiveness between plants infested by n. lugens nymphs or those infested by gravid females. attraction was correlated with time after i ... | 2005 | 16195848 |
| insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach-potato aphid, myzus persicae (hemiptera: aphididae) and the rice brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | the sfi1/gna fusion protein, comprising of snowdrop lectin (galanthus nivalis agglutinin, gna) fused to an insecticidal spider venom neurotoxin (segestria florentina toxin 1, sfi1) was tested for toxicity against the rice brown planthopper nilaparvata lugens (stål) and the peach-potato aphid myzus persicae (sulzer) by incorporation into artificial diets. significant effects on the mortality of n. lugens were observed, with 100% of the insects fed on the sfi1/gna fusion protein diet dead by day 7 ... | 2006 | 16206236 |
| sequencing and characterization of a cdna encoding a ferritin subunit of colorado potato beetle, leptinotarsa decemlineata. | a differentially expressed cdna fragment (p311) from colorado potato beetle (cpb), leptinotarsa decemlineata (say), was identified by restriction fragment differential display-polymerase chain reaction (rfdd-pcr) technique, and showed a strong similarity to ferritin heavy chain subunits of other organisms. based on p311, we constructed specific primers and obtained a 840-bp cdna fragment spanning the open reading frame of cpb ferritin subunit using the rapid amplification of cdna ends (race) tec ... | 2005 | 16235258 |
| high-resolution mapping of a new brown planthopper (bph) resistance gene, bph18(t), and marker-assisted selection for bph resistance in rice (oryza sativa l.). | brown planthopper (bph) is a destructive insect pest of rice in asia. identification and the incorporation of new bph resistance genes into modern rice cultivars are important breeding strategies to control the damage caused by new biotypes of bph. in this study, a major resistance gene, bph18(t), has been identified in an introgression line (ir65482-7-216-1-2) that has inherited the gene from the wild species oryza australiensis. genetic analysis revealed the dominant nature of the bph18(t) gen ... | 2006 | 16240104 |
| identification of a rice gene (bph 1) conferring resistance to brown planthopper (nilaparvata lugens stal) using sts markers. | this study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (bph) biotype 1, using japonica type resistant lines. bulked segregant analyses were conducted using 520 rapd primers to identify rapd fragments linked to the bph resistance gene. eleven rapds were shown to be polymorphic amplicons between resistant and susceptible progeny. one of these primers, ope 18, which amplified a 923 bp band tightly linked to resistance, was converted int ... | 2005 | 16258238 |