Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| nitrogen-fixing nodules with ensifer adhaerens harboring rhizobium tropici symbiotic plasmids. | ensifer adhaerens is a soil bacterium that attaches to other bacteria and may cause lysis of these other bacteria. based on the sequence of its small-subunit rrna gene, e. adhaerens is related to sinorhizobium spp. e. adhaerens atcc 33499 did not nodulate phaseolus vulgaris (bean) or leucaena leucocephala, but with symbiotic plasmids from rhizobium tropici cfn299 it formed nitrogen-fixing nodules on both hosts. the nodule isolates were identified as e. adhaerens isolates by growth on selective m ... | 2001 | 11425750 |
| sinorhizobium morelense sp. nov., a leucaena leucocephala-associated bacterium that is highly resistant to multiple antibiotics. | sinorhizobium morelense sp. nov. is described to designate a group of bacteria isolated from root nodules of leucaena leucocephala. s. morelense shows 98% 16s rrna gene sequence similarity to some sinorhizobium species and to ensifer adhaerens. this novel species is distinguished from other sinorhizobium species and from e. adhaerens by dna-dna hybridization, 165 rrna gene restriction fragments and sequence and some distinctive phenotypic features. strains of this species are highly resistant to ... | 2002 | 12361275 |
| description of new ensifer strains from nodules and proposal to transfer ensifer adhaerens casida 1982 to sinorhizobium as sinorhizobium adhaerens comb. nov. request for an opinion. | a group of four diverse rhizobial isolates and two soil isolates that are highly related to ensifer adhaerens were characterized by a polyphasic approach. on the basis of dna-dna hybridizations and phenotypic features, these strains cannot be distinguished clearly form ensifer adhaerens, a soil bacterium that was described in 1982, mainly on the basis of phenotypic characteristics. phylogenetically, ensifer and sinorhizobium form a single group in the 16s rdna dendrogram of the alpha-proteobacte ... | 2003 | 12892151 |
| the genus name ensifer casida 1982 takes priority over sinorhizobium chen et al. 1988, and sinorhizobium morelense wang et al. 2002 is a later synonym of ensifer adhaerens casida 1982. is the combination "sinorhizobium adhaerens" (casida 1982) willems et al. 2003 legitimate? request for an opinion. | the synonymy of the genera ensifer and sinorhizobium was recently reported, but it was proposed that the later-named genus, sinorhizobium, take priority in nomenclature. there is no justification in the international code of nomenclature of bacteria (prokaryotes) for this step; ensifer is the correct name of the genus, with ensifer adhaerens as the type species. species previously allocated to sinorhizobium are here proposed as the new combinations ensifer arboris, ensifer fredii, ensifer kostie ... | 2003 | 14657154 |
| ensifer adhaerens predatory activity against other bacteria in soil, as monitored by indirect phage analysis. | an indirect phage analysis procedure was used to detect and follow the activity of the bacterial predator ensifer adhaerens in situ in natural soil. the soil was percolated with an aqueous suspension of washed bacterial host cells so that the e. adhaerens cells naturally present in the soil would multiply in response to the host cells. the natural phage development which ensued against these multiplying e. adhaerens cells in the soil was then monitored by noting plaques which developed when the ... | 1983 | 16346275 |
| study of bacillus subtilis endospores in soil by use of a modified endospore stain. | the schaeffer-fulton endospore stain was modified so that it would stain bacillus subtilis endospores in soil smears. the modified stain differentiated among dormant spores, spores undergoing activation, and spores which had germinated but had not yet shown outgrowth. these differentiations were seen for spores in soil and for pure spore preparations in the laboratory. this stain was used to show reversible b. subtilis spore activation promoted by an ensifer adhaerens-like indigenous bacterium i ... | 1985 | 16346801 |
| catabolism of 1,5-anhydro-d-fructose in sinorhizobium morelense s-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-d-fructose reductase and its application in sugar analysis and rare sugar synthesis. | the bacterium sinorhizobium morelense s-30.7.5 was isolated by a microbial screening using the sugar 1,5-anhydro-d-fructose (af) as the sole carbon source. this strain metabolized af by a novel pathway involving its reduction to 1,5-anhydro-d-mannitol (am) and the further conversion of am to d-mannose by c-1 oxygenation. growth studies showed that the af metabolizing capability is not confined to s. morelense s-30.7.5 but is a more common feature among the rhizobiaceae. the af reducing enzyme wa ... | 2006 | 16461673 |
| occurrence of rhizobia in the gut of the higher termite nasutitermes nigriceps. | wood-eating termites feed on a diet highly deficient in nitrogen. they must complement their diet with the aid of nitrogen-fixing bacteria. nitrogen fixation in the gut has been demonstrated, but information about nitrogen-fixing bacteria in pure culture is scarce. from the higher termite nasutitermes nigriceps the symbiotic bacterial strain m3a was isolated, which thrives in the hindgut contents. the gram-negative strain exhibited similarities to the species of the genus ensifer (including sino ... | 2007 | 16584862 |
| crystal structure of nadp(h)-dependent 1,5-anhydro-d-fructose reductase from sinorhizobium morelense at 2.2 a resolution: construction of a nadh-accepting mutant and its application in rare sugar synthesis. | recombinant 1,5-anhydro-d-fructose reductase (afr) from sinorhizobium morelense s-30.7.5 was crystallized in complex with the cofactor nadp(h) and its structure determined to 2.2 a resolution using selenomethionine sad (refined r(work) and r(free) factors of 18.9 and 25.0%, respectively). as predicted from the sequence and shown by the structure, afr can be assigned to the gfo/idh/moca protein family. afr consists of two domains. the n-terminal domain displays a rossmann fold and contains the co ... | 2006 | 16906761 |
| multilocus sequence analysis of ensifer and related taxa. | multilocus sequence analysis (mlsa) was performed on representatives of ensifer (including species previously assigned to the genus sinorhizobium) and related taxa. neighbour-joining (nj), maximum-parsimony (mp) and maximum-likelihood (ml) phylogenies of dnak, glta, glna, reca, thrc and 16s rrna genes were compared. the data confirm that the potential for discrimination of ensifer species is greater using mlsa of housekeeping genes than 16s rrna genes. in incongruence-length difference tests, th ... | 2007 | 17329774 |
| multilocus sequence analysis of root nodule isolates from lotus arabicus (senegal), lotus creticus, argyrolobium uniflorum and medicago sativa (tunisia) and description of ensifer numidicus sp. nov. and ensifer garamanticus sp. nov. | nine isolates from argyrolobium uniflorum, lotus creticus , medicago sativa (tunisia) and lotus arabicus (senegal) were analysed by multilocus sequence analysis (mlsa) of five housekeeping genes (reca, atpd, glna, glta and thrc), the 16s rrna gene and the nodulation gene noda. analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, a, b and c, ... | 2010 | 19656922 |
| biodegradation of diphenylarsinic acid to arsenic acid by novel soil bacteria isolated from contaminated soil. | microorganisms capable of degrading diphenylarsinic acid (dpaa) were enriched from contaminated soil using the soil-charcoal perfusion method. two novel bacterial strains, l2406 and l2413, that can degrade dpaa in a mineral salt medium supplemented with dpaa as the sole carbon source were isolated. based on comparative morphology, physiology, and comparison of the 16s rrna gene sequences, both were presumed to be species closely related to ensifer adhaerens. as the metabolites, phenylarsonic aci ... | 2010 | 19949836 |
| why two are not enough: degradation of p-toluenesulfonate by a bacterial community from a pristine site in moorea, french polynesia. | in previous work, only one culture (strain ta12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (tsa) as a sole source of carbon and energy for aerobic growth. 'strain ta12' has now been recognized as a community of three bacteria: achromobacter xylosoxidans ta12-a, ensifer adhaerens ta12-b and pseudomonas nitroreducens ta12-c. achromobacter xylosoxidans ta12-a and e. adhaerens ta12-b were identified as the tsa degraders. these two organisms contain sever ... | 2011 | 21204940 |
| [isolation and degradation characteristics of acetochlor-degrading strain a-3]. | the strain a-3 which could use acetochlor as the sole nitrogen source was isolated from soils contaminated with acetochlor and the sludge of pesticide factory by enrichment culture. the strain was identified as ensifer adhaerens according to the results of morphology, physiology,and the phylogenetical analyses of 16s rrna sequence. it was observed that the degradation rate of acetochlor by strain a-3 was 33.6% after 10 days culture at the concentration of 10 mg/l. the composition of the culture ... | 2011 | 21528581 |
| alliinase from ensifer adhaerens and its use for generation of fungicidal activity. | abstract: a bacterium ensifer adhaerens ferm p-19486 with the ability of alliinase production was isolated from a soil sample. the enzyme was purified for characterization of its general properties and evaluation of its application in on-site production of allicin-dependent fungicidal activity. the bacterial alliinase was purified 300-fold from a cell-free extract, giving rise to a homogenous protein band on polyacrylamide gel electrophoresis. the bacterial alliinase (96 kda) consisted of two id ... | 2011 | 21906328 |
| production of phytophthora infestans-resistant potato (solanum tuberosum) utilising ensifer adhaerens ov14. | based on the use of agrobacterium tumefaciens-mediated transformation commodity crop improvement through genetic engineering is the fastest adopted crop technology in the world (james 2010). however, the complexity of the agrobacterium patent landscape remains a challenge for non-patent holders who wish to generate novel varieties for a commercial purpose. the potential of non-agrobacterium strains (transbacter(™)) to modify a plant genome has previously been described. however, they are unlikel ... | 2011 | 21912851 |
| isolation and identification of cellulolytic bacteria from the gut of holotrichia parallela larvae (coleoptera: scarabaeidae). | in this study, 207 strains of aerobic and facultatively anaerobic cellulolytic bacteria were isolated from the gut of holotrichia parallela larvae. these bacterial isolates were assigned to 21 genotypes by amplified ribosomal dna restriction analysis (ardra). a partial 16s rdna sequence analysis and standard biochemical and physiological tests were used for the assignment of the 21 representative isolates. our results show that the cellulolytic bacterial community is dominated by the proteobacte ... | 2012 | 22489111 |
| biological oxidation of arsenite in synthetic groundwater using immobilised bacteria. | biological oxidation of arsenite (as(iii)) in synthetic groundwater was examined by using arsenite oxidising bacteria (aob) isolated from an activated sludge. the phylogenetic analysis indicated that the isolated aob was closely related to ensifer adhaerens. batch experiments showed that for an as(iii) oxidation with the isolated aob the optimum ratio of nitrogen source (nh₄-n) concentration to as(iii) concentration was 0.5 (52 mg/l-110 mg/l) and the isolated aob preferred ph values ranging from ... | 2012 | 22760058 |
| optimization of exopolysaccharide production and diesel oil emulsifying properties in root nodulating bacteria. | bioremediation, a strategy mediated by microorganisms, is a promising way used in the degradation or removal of organic contaminants from soil or aquatic system. exopolysaccharide (eps) which was produced by a variety of gram-negative bacteria has been demonstrated to be a potential bioemulsifier used in the degradation of hydrocarbons. in the present study, attempts were made to optimize the production of eps from our newly isolates by adjusting the culture conditions and medium components. bes ... | 2011 | 22805917 |
| distribution and diversity of acyl homoserine lactone producing bacteria from four different soils. | the distribution and diversity of acyl homoserine lactone (ahl) producing bacteria in black, brown, red, and meadow soils were investigated using culture-dependent method. one out of seventy six and five out of fifty isolates from the black and the brown soil were ahl-biosensor active, respectively. they were affiliated to the genera ensifer and pseudomonas and the family enterobacteriaceae. thin layer chromatography showed that the most of them produced ahls with acyl side chain of 6 or 8 carbo ... | 2013 | 23007525 |
| isolation and characterization of acyl homoserine lactone-producing bacteria during an urban river biofilm formation. | the presence and diversity of acyl homoserine lactone (ahl)-producers in an urban river biofilm were investigated during 60-day biofilm formation. ahl biosensors detected the presence of ahl-producers in 1-60-day river biofilms. screening for ahl-producers resulted in 17 aeromonas spp., 3 pseudomonas spp., 3 ensifer spp., and 1 acinetobacter sp. among these isolates, six of them were closely related to acinetobacter tjernbergiae, aeromonas allosaccharophila, aeromonas aquariorum, aeromonas janda ... | 2012 | 23090571 |
| identification and phylogenetic characterization of cobalamin biosynthetic genes of ensifer adhaerens. | ensifer adhaerens csba was screened as a cobalamin producer. the draft genome sequence revealed that the strain possesses 22 cobalamin biosynthetic genes (cob genes). the cob gene arrangement on the genome of e. adhaerens csba was similar to that of other ensifer species, and most similar to that of pseudomonas denitrificans sc510. the cobn sequence phylogeny was generally congruent with that of the 16s rrna gene, and it is suggeted that e. adhaerens csba might have inherited the cob genes from ... | 2012 | 23257908 |
| biodegradation of the neonicotinoid insecticide thiamethoxam by the nitrogen-fixing and plant-growth-promoting rhizobacterium ensifer adhaerens strain tmx-23. | thiamethoxam (thia), a second generation neonicotinoid insecticide in the thianicotinyl subclass, is used worldwide. environmental studies revealed that microbial degradation is the major mode of removal of this pesticide from soil. however, microbial transformation of thia is poorly understood. in the present study, we isolated a bacterium able to degrade thia from rhizosphere soil. the bacterium was identified as ensifer adhaerens by its morphology and 16s ribosomal dna sequence analysis. high ... | 2012 | 23274958 |
| proposal of ensifer psoraleae sp. nov., ensifer sesbaniae sp. nov., ensifer morelense comb. nov. and ensifer americanum comb. nov. | in a survey of rhizobia associated with the native legumes in yunnan province, china, seven and nine strains isolated from the root nodules of psoralea corylifolia, sesbania cannabina and medicago lupulina were respectively classified into the novel genomic species groups i and ii in the genus ensifer (former sinorhizobium) based on the sequence analyses of the 16s rrna gene. analyses of concatenated housekeeping genes (atpd, reca and glnii) further revealed that they were distinct lineages in t ... | 2013 | 23759600 |
| ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea (cicer arietinum l.). | the taxonomic position of strain mssrfbl1(t), isolated from chickpea rhizosphere soil from kannivadi, india, was determined. strain mssrfbl1(t) formed bluish black colonies, stained gram-negative and was motile, aerobic, capable of fixing dinitrogen, oxidase-negative and catalase-positive. q-10 was the major respiratory quinone. major fatty acids of strain mssrfbl1(t) were c18 : 1ω7c and c19 : 0cycloω8c. minor amounts of c18 : 0, c12 : 0, c14 : 0 3-oh, c18 : 0 3-oh, c16 : 0, c16 : 1ω6c/c16 : 1ω7 ... | 2013 | 23907221 |
| isolation of an indigenous imidacloprid-degrading bacterium and imidacloprid bioremediation under simulated in situ and ex situ conditions. | the bacterial community structure and its complexity of the enrichment culture during the isolation and screening of imidacloprid-degrading strain were studied using denaturating gradient gel electrophoresis analysis. the dominant bacteria in the original tea rhizosphere soil were uncultured bacteria, rhizobium sp., sinorhizobium, ochrobactrum sp., alcaligenes, bacillus sp., bacterium, klebsiella sp., and ensifer adhaerens. the bacterial community structure was altered extensively and its comple ... | 2013 | 23985542 |
| invasion of rhizobial infection thread by non-rhizobia for colonization of vigna radiata root nodules. | legumes develop symbiotic relationships with rhizobium by a complex exchange of signals. despite the high specificity between symbiotic partners, the presence of non-rhizobial bacteria in root nodules has been reported. to investigate how these rhizobacteria enter root nodules, fluorescently tagged pseudomonas fluorescens and klebsiella pneumoniae were co-inoculated with host-nodulating ensifer adhaerens to vigna radiata seedlings and root hair infection was monitored using confocal microscopy a ... | 2013 | 24033808 |
| genome sequence of ensifer adhaerens ov14 provides insights into its ability as a novel vector for the genetic transformation of plant genomes. | recently it has been shown that ensifer adhaerens can be used as a plant transformation technology, transferring genes into several plant genomes when equipped with a ti plasmid. for this study, we have sequenced the genome of ensifer adhaerens ov14 (ov14) and compared it with those of agrobacterium tumefaciens c58 (c58) and sinorhizobium meliloti 1021 (1021); the latter of which has also demonstrated a capacity to genetically transform crop genomes, albeit at significantly reduced frequencies. | 2014 | 24708309 |
| the metabolism of neonicotinoid insecticide thiamethoxam by soil enrichment cultures, and the bacterial diversity and plant growth-promoting properties of the cultured isolates. | a soil enrichment culture (sec) rapidly degraded 96% of 200 mg l(-1) neonicotinoid insecticide thiamethoxam (tmx) in msm broth within 30 d; therefore, its metabolic pathway of tmx, bacterial diversity and plant growth-promoting rhizobacteria (pgpr) activities of the cultured isolates were studied. the sec transformed tmx via the nitro reduction pathway to form nitrso, urea metabolites and via cleavage of the oxadiazine cycle to form a new metabolite, hydroxyl clo-tri. in addition, 16s rrna gene- ... | 2014 | 24762175 |
| arsenite oxidation by a facultative chemolithoautotrophic sinorhizobium sp. kgo-5 isolated from arsenic-contaminated soil. | a chemolithoautotrophic arsenite-oxidizing bacterium, designated strain kgo-5, was isolated from arsenic-contaminated industrial soil. strain kgo-5 was phylogenetically closely related with sinorhizobium meliloti with 16s rrna gene similarity of more than 99%, and oxidized 5 mm arsenite under autotrophic condition within 60 h with a doubling time of 3.0 h. additions of 0.01-0.1% yeast extract enhanced the growth significantly, and the strain still oxidized arsenite efficiently with much lower do ... | 2014 | 25051896 |
| distribution and diversity of rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in northwest china. | a total of 155 nodule isolates that originated from seven sites in northwest china were characterized by pcr-rflp of the 16s rrna gene and sequence analysis of multiple core genes (16s rrna, reca, atpd, and glnii) in order to investigate the diversity and biogeography of glycine soja-nodulating rhizobia. among the isolates, 80 were ensifer fredii, 19 were ensifer morelense, 49 were rhizobium radiobacter, and 7 were putative novel rhizobium species. the phylogenies of e. fredii and e. morelense i ... | 2014 | 25052953 |
| taxonomy of rhizobia and agrobacteria from the rhizobiaceae family in light of genomics. | phylogenomic analyses showed two major superclades within the family rhizobiaceae that corresponded to the rhizobium/agrobacterium and shinella/ensifer groups. within the rhizobium/agrobacterium group, four highly supported clades were evident that could correspond to distinct genera. the shinella/ensifer group encompassed not only the genera shinella and ensifer but also a separate clade containing the type strain of rhizobium giardinii. ensifer adhaerens (casida a(t)) was an outlier within its ... | 2015 | 25660942 |
| profiling antibiotic resistance and electrotransformation potential of ensifer adhaerens ov14; a non-agrobacterium species capable of efficient rates of plant transformation. | ensifer adhaerens ov14 underpins the successful crop transformation protocol termed ensifer-mediated transformation but issues exist in regard to addressing the pleomorphic tendency of the bacterium in suboptimal conditions, identifying the optimal parameters for electrotransformation and defining the strain's antibiotic profile. here, modifications made to growth medium composition addressed the pleomorphic trait of e. adhaerens ov14, delivering uniform e. adhaerens ov14 growth to ensure effici ... | 2015 | 26253576 |
| characterization of the biosorption and biodegradation properties of ensifer adhaerens: a potential agent to remove polychlorinated biphenyls from contaminated water. | ensifer adhaerens is a soil bacterium known for its potential to remove pollutants from the environment. we investigated the contributions of biosorption and biodegradation to the process of polychlorinated biphenyl (pcb) removal from water by living or heat-killed e. adhaerens with different incubation times. we examined the physicochemical properties of e. adhaerens, including its membrane surface moieties, extracellular polymeric substances, and defense-related enzyme activities. in addition, ... | 2016 | 26476319 |
| ensifer-mediated transformation: an efficient non-agrobacterium protocol for the genetic modification of rice. | while agrobacterium-mediated transformation (amt) remains the most widely used technique for gene transfer in plants, interest exists for the use of non-agrobacterium gene delivery systems due to freedom-to-operate issues that remain with amt across several jurisdictions. in addition, the plant pathogenic mode of action of agrobacterium tumefaciens significantly increases the costs to passage engineered cultivars through the regulatory process. ensifer adhaerens (ov14) is a soil-related bacteriu ... | 2015 | 26543735 |
| draft genome sequence of ensifer adhaerens m78, a mineral-weathering bacterium isolated from soil. | ensifer adhaerens m78, a bacterium isolated from soil, can weather potash feldspar and release fe, si, and al from rock under nutrient-poor conditions. here, we report the draft genome sequence of strain m78, which may facilitate a better understanding of the molecular mechanism involved in mineral weathering by the bacterium. | 2016 | 27609930 |
| microbial degradation of polyhydroxyalkanoates with different chemical compositions and their biodegradability. | the study addresses degradation of polyhydroxyalkanoates (pha) with different chemical compositions-the polymer of 3-hydroxybutyric acid [p(3hb)] and copolymers of p(3hb) with 3-hydroxyvalerate [p(3hb/3hv)], 4-hydroxybutyrate [p(3hb/4hb)], and 3-hydroxyhexanoate [p(3hb/3hhx)] (10-12 mol%)-in the agro-transformed field soil of the temperate zone. based on their degradation rates at 21 and 28 °c, polymers can be ranked as follows: p(3hb/4hb) > p(3hb/3hhx) > p(3hb/3hv) > p(3hb). the microbial commu ... | 2017 | 27623963 |
| multi locus sequence analysis and symbiotic characterization of novel ensifer strains nodulating tephrosia spp. in the indian thar desert. | phylogenetically diverse ensifer strains associated with five species of tephrosia growing in alkaline soils of semi-arid regions of the thar desert were characterized using multi locus sequence analysis. based on 16s rrna and four protein-coding housekeeping gene (reca, atpd, glnii and dnak) sequences, the tephrosia-ensifer strains were genetically different from the type strains of ensifer saheli, ensifer kostiensis, ensifer terangae (african origin) and ensifer psoraleae (asiatic origin). one ... | 2016 | 27771162 |
| use of endophytic and rhizosphere bacteria to improve phytoremediation of arsenic-contaminated industrial soils by autochthonous betula celtiberica. | the aim of this study was to investigate the potential of indigenous arsenic-tolerant bacteria to enhance arsenic phytoremediation by the autochthonous pseudometallophyte betula celtiberica the first goal was to perform an initial analysis of the entire rhizosphere and endophytic bacterial communities of the above-named accumulator plant, including the cultivable bacterial species. b. celtiberica's microbiome was dominated by taxa related to flavobacteriales, burkholderiales, and pseudomonadales ... | 2017 | 28188207 |