Publications

sensitivity threshold of labellar and tarsal hairs of dacus oleae gmel. and ceratitis capitata wied. to nacl stimulation.		1978	751664
the electrophysiological response of labellar and tarsal hairs of dacus oleae gmel. to salt and sugar stimulation.		1978	751666
[research on some phosphorganic insecticide residues in olive oil in relation to techniques of dacus oleae control. i. results of total treatment to the foliage (author's transl)].	the research conducted to verify the presence and the amount of residues of organophosphorus insecticides in olive oil from olive trees sprayed against. dacus oleae has shown that both hydrophilic (dimethoate, formathion, phosphamidon) and lypophilic (fenthion, medathion, prolate) substances tested leave more or less appreciable residues in the oil, either in a short time or in more than one month from the treatment. in the operative conditions reflecting antidacic practice, the amount of residu ...	2008	1037421
how many genes are selected in populations of dacus oleae.	three natural populations of dacus oleae have been sampled for six electrophoretically detected polymorphic genes. the distribution of the allele frequencies in the different populations were analyzed by the method suggested by lewontin and krakauer (1973) (the various in space one) in order to test for selective neutrality. this method, however, which in our case showed that one or more genes are or have been subjected to selection, does not permit estimation of the number of selected loci. an ...	1975	1132685
mitotic and polytene chromosome analysis in dacus oleae (diptera: tephritidae).	the present study constitutes the first attempt to construct a photographic map of the polytene chromosomes of dacus oleae, a pest of the olive tree that causes serious financial damage in all olive oil producing countries. the map was constructed by using the larval fat body cells, the chromosomes of which are representative of the polytene chromosomes of other polytene tissues. in addition, the mitotic chromosomes of brain ganglia were examined, permitting tentative correlations between mitoti ...	1992	1624130
toxicity of bacillus thuringiensis to laboratory populations of the olive fruit fly (dacus oleae).	a survey of bacillus thuringiensis recovered from the environments of olive groves in greece was carried out. of 80 soil samples, 24 were found to contain b. thuringiensis with parasporal crystal inclusions; these were tested for toxicity against the olive fruit fly (dacus oleae). mortality levels of larvae caused by the different isolates varied from 7 to 87%. higher levels of mortality were observed if a mixture of relatively pure crystals and spores was used compared with the mortality result ...	1991	1768100
structure and morphogenesis of the eggshell and micropylar apparatus in the olive fly, dacus oleae (diptera: tephritidae).	the egg of the olive fly, dacus oleae (diptera, tephritidae), is laid inside olives and the larva eventually destroys the fruit. the oocyte is surrounded by several distinct layers which are produced during choriogenesis. the chorion covering the main body of the egg outside of the vitelline membrane includes a "wax" layer, an innermost chorionic layer, an endochorion consisting of inner and outer layers separated by pillars and cavities similar to their counterparts in drosophila melanogaster, ...	1991	1920445
the urea-soluble low molecular weight cuticle proteins from the different developmental stages of dacus oleae.	the cuticle proteins of the insect dacus oleae have been isolated by extraction with a solution of 7 m urea. the affinity properties of cuticle proteins, isolated from the third instar larvae (l3dcps 1-7), to chitin have been studied. purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. the developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis.	1990	2224572
bidirectional swimming in spermatozoa of tephritid flies.	our observations show that spermatozoa of the mediterranean fruit fly ceratitis capitata and of dacus oleae and dacus dorsalis are capable of swimming backwards as well as forwards, and that they can change direction abruptly. the preferred direction is backwards, observed in spermatozoa obtained from the male genitalia. forwards swimming spermatozoa were frequently seen in the spermatheca and close to the eggs. the change in swimming direction appears to be effected solely by a change in the di ...	1989	2804950
dacus oleae microbial symbionts.	dacus oleae (gmelin), which is a major insect pest of the olive tree, is closely associated during all developmental stages with a bacterial symbiont. this review describes the localization and transmission of the symbiont, the attempts made to isolate, cultivate and characterize it, and the host-symbiont interactions.	1988	3079231
an inapparent infection with a probable picornavirus in several stocks of laboratory reared and naturally occurring populations of dacus oleae gmel. pupae in greece.	pupae from several stocks of wild and laboratory reared olive fruit fly, dacus oleae, were fractionated by a series of steps designed to identify occluded and nonoccluded viruses. two different size of particles were isolated, the smaller of which contained a single-stranded rna molecule of about 2.8 x 10(6). this small rna virus was found to inapparently infect a ceratitis capitata continuous cell line.	1987	3657601
free and peptide-bound amino acids in the insect dacus oleae (gmelin) grown under natural and artificial conditions.		1972	4662570
metepa sterilization of the olive fruit fly.		1973	4690297
biochemistry of the insects dacus oleae and ceratitis capitata. changes of cholinesterases and triglyceride hydrolyzing enzymes.		1973	4709972
distribution of gene frequency as a test of the theory of the selective neutrality of polymorphisms.	the variation in gene frequency among populations or between generations within a population is a result of breeding structure and selection. but breeding structure should affect all loci and alleles in the same way. if there is significant heterogeneity between loci in their apparent inbreeding coefficients f=s(p) (2)/p(1-p), this heterogeneity may be taken as evidence for selection. we have given the statistical properties of f and shown how tests of heterogeneity can be made. using data from ...	1973	4711903
the genetics of dacus oleae. 3. amount of variation at two esterase loci in a greek population.		1969	5367376
lipids of the adult olive fruit fly. total lipids and major lipid fractions.		1969	5374166
mating frequency and inseminating capacity of radiation-sterilized and normal males of the olive fruit fly.		1970	5449394
biochemistry of the development of the insect dacus oleae: evolution of fatty acid composition of different lipid classes.		1970	5492194
biochemistry of the development of the insects dacus oleae and ceratitis capitata-evolution of total fatty acids.		1969	5777373
[the fight against the olive fly. possibility of use, from the hygienic-sanitary point of view, of the n-monomethylamide of o,o-dimethyldithiophosphorylacetic acid (rogor) and of o,o-dimethyl-1-hydroxy-2,2,2-trichloroethylphosphate (dipterex)].		1966	5964032
amino-acid synthesis in adult dacus oleae (gmelin) (diptera tephritidae) determined with [u-14c] glucose.	the ability of adult dacus oleae for amino-acid synthesis from [u-14c] glucose was investigated. the relatively high specific activity radiometric measurements indicated that both sexes were able to synthesize the amino acids: alanine, aspartic acid, cystine, glutamic acid, glycine, hydroxyproline, proline and tyrosine; therefore, these amino acids are considered as nutritionally dispensable for d. oleae. on the other hand, the amino acids: arginine, histidine, leucine, isoleucine, lysine, methi ...	1984	6085250
effects of varying the dietary nitrogen to carbohydrate ratio upon the biological performance of adult dacus oleae. (diptera tephritidae).	by varying the dietary proportions of nitrogen to carbohydrate in a chemically defined diet, it was found that the olive fruit adult fly cannot utilize amino nitrogen as an energy source, while the most critical ingredient for prolonged survival was the carbohydrate part of the diet. increase of the nitrogen contents above 2.6%, up to 6.4%, shortened adult life span by 50%. the best reproduction rate was scored under a n/c ratio of 1.6/40, while higher nitrogen contents reduced egg deposition. f ...	1981	6167227
the importance of dietary amino acids on the reproduction and longevity of adult dacus oleae (gmelin) (diptera tephritidae).	when the amino-acid mixture of an effective chemically defined diet was replaced by single amino acids, keeping the total nitrogen at the same level, the egg production of dacus oleae was minimal with all the 19 amino acids tested. male survival was adversely affected by the amino acids : alanine, aspartic acid, glutamic acid, glycine, hydroxyproline, lysine, serine and tyrosine, while female survival was shortened when the amino acids : glycine, hydroxyproline and lysine were added. the creatio ...	1983	6198985
the effect of gamma rays on the gonads of the olive fruit fly, dacus oleae (gmelin).	pupae of the olive fruit fly, dacus oleae (gmelin) 1 to 2 days before adult emergence were irradiated with the suitable sterilizing dose of 80 gy gamma rays. at intervals of 5, 10, 15, 20, 25 and 30 days after adult emergence, anatomical and biometrical studies were performed to determine the extent of recovery of d. oleae gonads during one month of adult life. there were some indications of gonad recovery after two weeks. this recovery was observed as a decrease in the percentage deviation from ...	1983	6600730
isolation and partial characterization of two alcohol dehydrogenase isozymes from the medfly ceratitis capitata.	two alcohol dehydrogenase isozymes, namely adh-1 and adh-2 from ceratitis capitata were purified to homogeneity and further characterized. after ammonium sulphate precipitation from an extract of whole third instar larvae, the two isozymes were separated by ion exchange chromatography on q-sepharose. a combination of affinity chromatography, gel filtration and ion exchange chromatography was then used to purify each isozyme (50 and 57 times with 53 and 58% yields, for adh-1 and -2 respectively). ...	1994	8111424
inclusion complexes of cyclomaltoheptaose (beta-cyclodextrin) and its methylated derivatives with the main components of the pheromone of the olive fruit fly.	the inclusion complexes of cyclomaltoheptaose (beta cd) and heptakis(2,3,6-tri-o-methyl)cyclomaltoheptaose (tm-beta cd) with the four major components of the pheromone of the olive fruit fly (dacus oleae), namely 1,7-dioxaspiro[5.5]undecane, (-)-alpha-pinene, nonanal, and ethyl dodecanoate, and the complex of heptakis(2,6-di-o-methyl)cyclomaltoheptaose (dm-beta cd) with 1,7-dioxaspiro[5.5]undecane were studied. the complexes were characterised in the solid state by differential scanning calorime ...	1993	8472260
enzymatic preparation of (4r,6r)-4-hydroxy-1,7-dioxaspiro[5.5]undecane and its antipode, the minor component of the olive fruit fly pheromone.		1996	8733619
inclusion of the main pheromone component of dacus oleae, 1,7-dioxaspiro[5,5]undecane, in beta-cyclodextrin.	the complex beta-cyclodextrin-1,7-dioxaspiro[5,5]undecane nonahydrate, c42h70o35.c9h16o2.9h2o, belongs to the class of beta-cyclodextrin dimeric-type complexes. the racemic guest molecule is present in a disordered position. both enantiomers are located in two different regions inside the channel formed by the host dimers.	1996	8962447
a nonoccluded reovirus of the olive fly, dacus oleae.	we have isolated paraspherical viral particles, 60 nm in diameter, from adults of the olive fly (dacus oleae) collected in greece. the virus actively replicated in midgut epithelial cells and in advanced infections virions accumulated in microvilli. they were released in the gut lumen and were very abundant in fly faeces. the virions exhibited the salient features of reoviruses, with an external shell and an internal core with a tubular subunit protruding at each vertex of the icosahedron. the v ...	1997	9010312
porphyrins and related compounds as photoactivatable insecticides. i. phototoxic activity of hematoporphyrin toward ceratitis capitata and bactrocera oleae.	the photodynamic sensitizer hematoporphyrin (hp) ix was efficiently accumulated by ceratitis capitata (mediterranean fruit fly) and bactrocera oleae (olive fly) when the insects were fed with a sugar/protein bait containing micromolar amounts of porphyrin. hematoporphyrin appeared to be mainly accumulated in the midgut, malpighian tubes, adipose tissue and cuticle and was gradually cleared from the organism in a 24-48 h time interval. exposure of the hp-fed flies to light mimicking the solar spe ...	1998	9487798
purification of alcohol dehydrogenase from four genotypes of the olive fruit fly bactrocera (dacus) oleae.	this is the first report describing the purification of alcohol dehydrogenase (adh) from four genotypes of the olive fruit fly bactrocera oleae, the most important pest of olives in the mediterranean region. the purified enzyme shows a single band after sds-page analysis, corresponding to subunit mass of 26 kda. the native adh shows a molecular mass of 48 kda, after gel filtration hplc analysis. the purification method incorporated a preliminary ammonium sulphate precipitation step, followed by ...	2003	9548783
biochemical differences between products of the adh locus in olive fruit fly (bactrocera oleae).	purified alcohol dehydrogenases from olive fruit flies of genotypes ss, ii, and si were biochemically compared. the enzymes were found to differ in the specific activity, in the influence of ph and temperature on activity, and in the affinity with different substrate-alcohols. the probable relationships of these findings with the dramatic changes in allele frequencies observed when natural populations are introduced in the laboratory are discussed.	1998	9791721
insecticidal activity of strains of bacillus thuringiensis on larvae and adults of bactrocera oleae gmelin (dipt. tephritidae).	the olive fly, bactrocera oleae, is the key pest on olives in the mediterranean area. the pest can destroy, in some cases, up to 70% of the olive production. its control relies mainly on chemical treatments, sometimes applied by aircraft over vast areas, with their subsequent ecological and toxicological side effects. bacillus thuringiensis is a spore-forming soil bacterium which produces a protein crystal toxic to some insects, including the orders of lepidoptera, diptera, and coleoptera and ot ...	1999	10486224
biochemical and immunocytochemical analysis of vitellogenesis in the olive fruit fly dacus (bactrocera) oleae (diptera: tephritidae).	synthesis and selective accumulation of the major yolk proteins in the developing oocytes of the species dacus oleae (diptera: tephritidae) was studied biochemically and by immunoelectron microscopy. in the hemolymph of adult females, two yolk proteins precursors (or vitellogenins) have been detected. they each exhibit a similar molecular weight and isoelectric point to their respective mature yolk proteins (or vitellins), while electrophoretic analysis of their synthetic profile shows that thei ...	1999	10623421
porphyrins and related compounds as photoactivatable insecticides. 3. laboratory and field studies.	the exposure of populations of ceratitis capitata (fruit fly), bactrocera oleae (olive fly) and stomoxis calcitrans (house fly) to a bait containing mumolar concentrations of porphyrin-type photosensitizers resulted in a significant accumulation of the porphyrin by the insects and a consequent development of photosensitivity upon exposure to visible light. the photoinsecticidal activity appeared to increase with increasing hydrophobicity of the porphyrin molecule: thus, the amphiphilic dicationi ...	2000	10687383
acquisition of a potential marker for insect transformation: isolation of a novel alcohol dehydrogenase gene from bactrocera oleae by functional complementation in yeast.	the alcohol dehydrogenase genes make up one of the best studied gene families in drosophila, both in terms of expression and evolution. moreover, alcohol dehydrogenase genes constitute potential versatile markers in insect transformation experiments. however, due to their rapid evolution, these genes cannot be cloned from other insect genera by dna hybridization or pcr-based strategies. we have therefore explored an alternative strategy: cloning by functional complementation of appropriate yeast ...	2000	10732677
sunlight-activated insecticides: historical background and mechanisms of phototoxic activity.	several photosensitizing agents, which are activated by illumination with sunlight or artificial light sources, have been shown to be accumulated in significant amounts by a variety of insects when they are administered in association with suitable baits. the subsequent exposure of such insects to uv/visible light leads to a significant drop in survival. of the photosensitizers tested so far, xanthenes (e.g. phloxin b) and porphyrins (e.g. haematoporphyrin) appear to be endowed with the highest ...	2000	10899458
correlation of the insecticidal activity of the bacillus thuringiensis a4 strain against bactrocera oleae (diptera) with the 140-kda crystal polypeptide.	the crystals of the soil-isolated bacillus thuringiensis (bt) strain a4 consist of two polypeptides with molecular mass of 140 kda and 32 kda that exhibit insecticidal activity against adult flies of bactrocera oleae (diptera). plasmid curing applied to this strain resulted in the isolation of several subclones exhibiting alterations in their crystal polypeptides as well as two acrystalliferous subclones. the crystals of subclone 1.1 lacked the 32-kda polypeptide and consisted uniquely of a 140- ...	2000	10977893
characterization of two alcohol dehydrogenase (adh) loci from the olive fruit fly, bactrocera (dacus) oleae and implications for adh duplication in dipteran insects.	we report the cloning and structural characterization of two adh loci of the olive fruit fly, bactrocera oleae. each of the two genes, named adh1 and adh2, consists of three exons and two introns for a total length of 1981 and 988 nucleotides, respectively. their deduced amino acid sequences of 257 and 258 residues exhibit a 77% identity and display the characteristics of the insect adh enzymes, which belong to the short-chain dehydrogenases/reductases family. the adh genes of b. oleae are compa ...	2001	11139292
actin cytoskeleton reorganization of the apoptotic nurse cells during the late developmental stages of oogenesis in dacus oleae.	in the present study, we demonstrate the actin cytoskeleton reorganization during nurse cells apoptosis of the olive fruit fly dacus oleae. at the developmental stage 9a of oogenesis, the actin microfilaments are assembled in numerous ring canals and subcortically support all the nurse cells, as is shown by phalloidin-fitc staining. during the following stages, 9b and 10a, this structural pattern remains the same. the developmental stage 10b is characterized by actin microfilament rearrangement ...	2001	11223953
the drosophila alcohol dehydrogenase gene may have evolved independently of the functionally homologous medfly, olive fly, and flesh fly genes.	cdnas for alcohol dehydrogenase (adh) isozymes were cloned and sequenced from two tephritid fruit flies, the medfly ceratitis capitata and the olive fly bactrocera oleae. because of the high sequence divergence compared with the drosophila sequences, the medfly cdnas were cloned using sequence information from the purified proteins, and the olive fly cdnas were cloned by functional complementation in yeast. the medfly peptide sequences are about 83% identical to each other, and the corresponding ...	2001	11230533
non-covalent interactions in the crystallization of the enantiomers of 1,7-dioxaspiro.	the enantiomers of racemic olive fly sex pheromone 1,7-dioxaspiro[5.5]undecane (1) have been isolated by crystallization with enantiospecific cyclodextrin hosts: (s)-(1) crystallizes with heptakis(2,3,6-tri-o-methyl)-beta-cyclodextrin (tmbetacd) and (r)-(1) with hexakis(2,3,6-tri-o-methyl)-alpha-cyclodextrin (tmalphacd). the crystal structure of tmbetacd/(s)-(1) from synchrotron radiation data at 100 k, determined for the first time, proves that tmbetacd crystallizes with only the (s)-enantiomer ...	2001	11373401
a pseudo-likelihood method for estimating effective population size from temporally spaced samples.	a pseudo maximum likelihood method is proposed to estimate effective population size (ne) using temporal changes in allele frequencies at multi-allelic loci. the computation is simplified dramatically by (1) approximating the multi-dimensional joint probabilities of all the data by the product of marginal probabilities (hence the name pseudo-likelihood), (2) exploiting the special properties of transition matrix and (3) using a hidden markov chain algorithm. simulations show that the pseudo-like ...	2001	11865714
classification and class-modeling of "riviera ligure" extra-virgin olive oil using chemical-physical parameters.	the protected designation of origin (pdo) "riviera ligure" for extra-virgin olive oils from liguria specifies three additional geographical mentions corresponding to three different geographical areas. to obtain a complete characterization of this typical italian product, 217 samples of olive oils produced in this north italian region during 1998/99 and 1999/2000 were analyzed. for each sample 31 variables were determined by chemical-physical analyses, and the data were subjected to a multivaria ...	2002	11929311
sex pheromone biosynthesis in the female olive fruit-fly. double labelling from [18o2]-dioxygen into 1,7-dioxaspiro[5.5]undecane.	the demonstration that both oxygen atoms of 1,7-dioxaspiro[5.5]undecane (1), the sex-pheromone of the female olive fly, originate from dioxygen, strongly implicates monooxygenase mediated processes in assembly of (1), and reveals unexpected complexity in the formation of its nine-carbon precursor.	2002	12109126
resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly bactrocera oleae.	a 2.2-kb full length cdna containing an orf encoding a putative acetylcholinesterase (ache) precursor of 673 amino acid residues was obtained by a combined degenerate pcr and race strategy from an organophosphate-susceptible bactrocera oleae strain. a comparison of cdna sequences of individual insects from susceptible and resistant strains, coupled with an enzyme inhibition assay with omethoate, indicated a novel glycine-serine substitution (g488s), at an amino acid residue which is highly conse ...	2002	12144698
effect of acetone feeding on alcohol dehydrogenase activity in the olive fruit fly, bactrocera oleae.	the purpose of this study is to demonstrate a clear connection between the presence of acetone in larval diet and alcohol dehydrogenase (adh) activity in laboratory raised populations of bactrocera oleae. adh activity of b. oleae is depressed in acetone-impregnated diets. at the same time the change of activity is accompanied by a change in the relative proportions of the multiple forms of adh. the bulk of activity in the most cathodally migrating form is lost, and all the activity becomes local ...	2002	12466988
genetic and cytogenetic analysis of the olive fruit fly bactrocera oleae (diptera: tephritidae).	the genetic and cytogenetic characteristics of one of the major agricultural pests, the olive fruit fly bactmcera oleae, are presented here. the mitotic metaphase complement of this insect consists of six pairs of chromosomes including one pair of heteromorphic sex chromosomes, with the male being the heterogametic sex. the analysis of the polytene complements of three larval tissues, the fat body, the salivary glands and the malpighian tubules of this pest has shown (a) a total number of five l ...	2002	12484525
tracing the history of an enzyme polymorphism: the case of alcohol dehydrogenase-2 (adh-2) of the olive fruit fly bactrocera oleae.	in the olive fruit fly bactrocera oleae, previous studies have described a one-locus three-allele electrophoretic polymorphism of the enzyme alcohol dehydrogenase and provided evidence that the polymorphism is under the influence of selection. a recent study has shown that this species carries a two-locus duplication for alcohol dehydrogenase. here, we show that the polymorphism maps at one of the duplicated loci, adh2, and identify the nucleotide and, therefore, the inferred amino acid differen ...	2003	12644551
modes of programmed cell death during ceratitis capitata oogenesis.	in the present study, we demonstrate the existence of two distinct apoptotic patterns in nurse cells during ceratitis capitata oogenesis. one is developmentally regulated and normally occurs during stages 12 and 13, and the other is stage specific and is sporadically observed during stages 7 and 8. the pre-apoptotic manifestation of the first pattern begins at stage 11 and is characterized by the formation of actin bundles. subsequently, at stages 12 and 13, the nurse cell nuclei exhibit condens ...	2003	12747933
the dual function of ovo/shavenbaby in germline and epidermis differentiation is conserved between drosophila melanogaster and the olive fruit fly bactrocera oleae.	the olive fruit fly bactrocera oleae (b. oleae) is a major olive damaging pest in the mediterranean area. as a first molecular analysis of a developmental gene in this insect, we characterised the ovo/shavenbaby (ovo/svb) gene. in drosophila, ovo/svb encodes a family of transcription regulators with two distinct functions: ovo is required for female germline differentiation and svb controls morphogenesis of epidermal cells. here, we report the cloning and characterisation of ovo/svb in b. oleae, ...	2003	12826096
[fly dacus oleae rossi in the province of latina].		1953	13134598
[winter and spring behavior of the olive fly (dacus oleae gmel.) in the province of latina and some standards of the campaign against the insect].		1954	13204669
[some control measures against the olive fly (dacus oleae gmel.)].		1955	13310823
[determination of residual insecticides used against dacus oleae in olive oil. i. parathion].		2015	13521559
[resistance to cold of dacus oleae gml].		1959	13658457
[histochemical and electron microscopy research on the malpighian tubules of dacus oleae gmel. i. the larva].		1963	13934255
insecticide residues in olive oils and table olives from efforts to control the olive fly.		1962	14011919
[insecticide residues and toxicological aspects in the control of the olive fly].		1962	14011920
[residual insecticides in olive oils from experimental spraying of olive trees against dacus oleae in 1958].		1963	14134245
[research on the amount of residues in olive oil of certain insecticides employed for the control of dacus oleae: b) diazinone].		2015	14472373
exploring the evolutionary history of the alcohol dehydrogenase gene (adh) duplication in species of the family tephritidae.	in the olive fruit fly bactrocera oleae and the med fly ceratitis capitata previous studies have shown the existence of two adh genes in each species. this observation, in combination with the former finding that various drosophila species of virilis and repleta group encode two isozymes of adh which are the result of a gene duplication, challenged us to address a scenario dealing with the evolutionary history of the adh gene duplication in tephritidae. in our lab we proceeded to the cloning and ...	2003	14562961
a high mobility group protein from the dipteran insects ceratitis capitata and bactrocera oleae.	nuclei from bactrocera oleae and ceratitis capitata larvae contain a major protein that shares most of the characteristics of vertebrate high mobility group (hmg) proteins. proteins are extracted from nuclei with 0.35 m nacl, are soluble in 5% perchloric acid, are relatively small (molecular weight in the range of 10-16 kda), and have both a high basic and a high acidic amino acid content. the amino acid constitution of these proteins is similar to that of the hmgb protein family of vertebrates. ...	2003	14587666
the mitochondrial genome of the olive fly bactrocera oleae: two haplotypes from distant geographical locations.	the complete sequence of the olive fly (bactrocera oleae) mitochondrial genome has been determined. two independent haplotypes, from flies of distant geographical origin (italy and portugal) were completely sequenced. the molecule is 15815 bp long, and shows the gene content and organization typical of insects, namely thirteen protein coding genes (pcgs) encoding proteins involved in oxidative phosphorylation, two rrnas, twenty-two trnas and a long (949 bp) noncoding region. the genomes of the t ...	2003	14986921
functional constraints of alcohol dehydrogenase (adh) of tephritidae and relationships with other dipteran species.	alcohol dehydrogenase is considered a very important enzyme in insect metabolism because it is involved (in its homodimeric form) in the catalysis of the reversible conversion of various alcohols in larval feeding sites to their corresponding aldehydes and ketones, thus contributing to detoxification and metabolic purposes. using 14 amino acid adh sequences recently determined in our laboratory, we constructed a three-dimensional (3d) model of olive fruit fly bactrocera oleae adh1 and adh2, base ...	2004	15170253
synthesis of haptens and development of an immunoassay for the olive fruit fly pheromone.	an enzyme-linked immunosorbent assay (elisa) for the olive fruit fly pheromone, bactrocera oleae gmelin, was developed. the assay uses polyclonal antibodies, raised in rabbits, against (+/-)-beta-[3-(1,7-dioxaspiro[5.5]undecane)]propionic acid, 2 (hapten i), conjugated to the klh (keyhole limpet hemocyanin) by the carbodiimide method. a second hapten, (+/-)-delta-[3-(1,7-dioxaspiro[5.5]undecane)]butylamine, 3 (hapten ii), after conjugation to a biotin moiety, was used for indirect immobilization ...	2004	15237938
quarantine strategies for olive fruit fly (diptera: tephritidae): low-temperature storage, brine, and host relations.	a dose-response relationship was not observed in olive fruit fly, bactrocera oleae (gmelin), larvae exposed to acetic acid concentrations (0-2.5%) used in commercial brine solutions to cure olives. immersion in a 1% acetic acid brine solution impeded emergence of the immature stages. a 1-wk exposure of olives infested with olive fruit fly larvae to low-temperature storage as a postharvest treatment at 0-1 degree c resulted in 8% survival of the population, and exposures of 2 through 5 wk further ...	2004	15384334
functional constraints of 6-phosphogluconate dehydrogenase (6-pgd) based on sequence and structural information.	the pentose phosphate cycle is considered as a major source of nadph and pentose needed for nucleic acid biosynthesis. 6-phosphogluconate dehydrogenase (6pgd), an enzyme participating in this cycle, catalyzes the oxidative decarboxylation of 6pgd to ribulose 5-phosphate with the subsequent release of co2 and the reduction of nadp. we have determined the amino acid sequence of 6pgd of bactrocera oleae and constructed a three-dimensional model based on the homologous known sheep structure. in a co ...	2004	15553090
spiroacetal biosynthesis: (+/-)-1,7-dioxaspiro[5.5]undecane in bactrocera cacuminata and bactrocera oleae (olive fruit fly).	[reaction: see text] a biosynthetic scheme rationalizing the formation of (+/-)-1,7-dioxaspiro[5.5]undecane (5) in the fruit fly species bactrocera cacuminata and bactrocera oleae (olive fruit fly) is presented. incorporation studies with deuterium-labeled keto aldehyde (10), 1,5-nonanediol (11), and 1,5,9-nonanetriol (12), and our previous finding that both oxygen atoms of 5 originate from dioxygen, are strongly evidentiary. the racemic condition of the natural spiroacetal 5 is accounted for, a ...	2005	15760167
isolation and characterization of the bactrocera oleae genes orthologous to the sex determining sex-lethal and doublesex genes of drosophila melanogaster.	here we report the isolation and characterization of the olive fruit fly bactrocera oleae genes orthologous to the drosophila melanogaster sex-determining genes sex-lethal (sxl) and doublesex (dsx). fragments of the sxl and dsx orthologous were isolated with rt-pcr. genomic and cdna clones were then obtained by screening a genomic library and separate male and female cdna adult libraries using the rt-pcr products as probes in both cases. b. oleae sxl gene (bosxl) expresses the same pattern of tr ...	2005	15777677
'candidatus erwinia dacicola', a coevolved symbiotic bacterium of the olive fly bactrocera oleae (gmelin).	the taxonomic identity of the hereditary prokaryotic symbiont of the olive fly bactrocera oleae (diptera: tephritidae) was investigated. in order to avoid superficial microbial contaminants and loosely associated saprophytic biota, flies were surface-sterilized at the larval stage and reared under aseptic conditions until adult emergence. b. oleae flies originating from different geographical locations and collected at different times of the year were tested. bacterial isolation was undertaken f ...	2005	16014495
population structure and colonization history of the olive fly, bactrocera oleae (diptera, tephritidae).	the olive fly, bactrocera oleae, is the major pest of olives in most commercial olive-growing regions worldwide. the species is abundant in the mediterranean basin and has been introduced recently into california and mexico, creating problems for quarantine protection and international trade. here, we use nuclear microsatellite markers and mitochondrial sequences to examine the history of olive fly range expansion and colonization. sampled populations span the current distribution of the olive f ...	2005	16029474
microsatellite analysis of olive fly populations in the mediterranean indicates a westward expansion of the species.	bactrocera oleae is the major insect pest of the olive fruit. twelve microsatellite loci isolated from the genome of this insect were used in a mediterranean-wide population analysis. these loci were highly polymorphic with a mean number of alleles per locus of 10.42 and a mean effective number of alleles of 2.76. the analysis was performed on a sample of 671 flies collected from nineteen locations around the european part of the mediterranean basin. despite the high level of gene flow across th ...	2005	16247695
cricket paralysis virus, a potential control agent for the olive fruit fly, dacus oleae gmel.	representatives of several families of insect viruses were tested for growth and pathogenicity in the olive fruit fly, dacus oleae gmel. the viruses included nuclear polyhedrosis viruses, an iridovirus, two picornaviruses, and trichoplusia ni small rna virus (a member of the nudaurelia beta family), in addition to two naturally occurring viruses of the olive fruit fly. two viruses, one of the two picornaviruses (cricket paralysis virus [crpv] and the iridovirus (type 21 from heliothis armigera), ...	1987	16347255
follicular atresia during dacus oleae oogenesis.	programmed cell death, constitutes a common fundamental incident that occurs during oogenesis in a variety of different animals. it plays a significant role in the maturation process of the female gamete and also in the removal of abnormal and superfluous cells at certain checkpoints of development. in the present study, we demonstrate the existence of follicular atresia during mid-oogenesis in the olive fruit fly dacus oleae (tephritidae). the number of atretic follicles increases following the ...	2006	16368106
cloning and structural characterization of the 6-phosphogluconate dehydrogenase locus of the medfly ceratitis capitata and the olive fruit fly bactrocera oleae.	the pentose phosphate cycle is considered as a major source of nadph and pentose needed for nucleic acid biosynthesis. 6-phosphogluconate dehydrogenase (6pgd), an enzyme participating in this cycle, catalyzes the oxidative decarboxylation of 6pgd to ribulose 5-phosphate with the subsequent release of co(2) and the reduction of nadp. we have determined the genomic sequences of 6pgd of two species of tephritidae, the medfly ceratitis capitata and olive fruit fly bactrocera oleae, and constructed a ...	2006	16459157
germ line transformation of the olive fly bactrocera oleae using a versatile transgenesis marker.	the olive fruit fly (olive fly) bactrocera oleae (dacus), recently introduced in north america, is the most destructive pest of olives worldwide. the lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its control. we have developed a minos-based transposon vector carrying a self-activating cassette which overexpresses the enhanced green fluorescent protein (egfp). efficient transposase-mediated integration ...	2006	16469073
microflora species and their volatile compounds affecting development of an alcohol dehydrogenase homozygous strain (adh-i) of bactrocera (dacus) oleae (diptera: tephritidae).	microflora species and volatiles emitted from artificial diets were examined from the larvae of three homozygous alcohol dehydrogenase (adh) strains of the olive fruit fly, bactrocera (dacus) oleae (gmelin), reared under identical conditions. differences in volatile composition were detected when adh-i homozygous larvae developed in a diet lacking the preservative p-hydroxybenzoic acid methyl ester (nipagin). larval development of the adh-i strain in the preservative-free diet was reduced by 50% ...	2005	16539118
programmed cell death of follicular epithelium during the late developmental stages of oogenesis in the fruit flies bactrocera oleae and ceratitis capitata (diptera, tephritidae) is mediated by autophagy.	in the present study, we describe the features of programmed cell death of ovarian follicle cells, occurring during the late developmental stages of oogenesis in the olive fruit fly, bactrocera oleae and the medfly, ceratitis capitata. during stage 14, the follicle cells contain autophagic vacuoles, and they do not exhibit caspase activity in all parts of the egg chamber. their nuclei are characterized by condensed chromatin, accompanied with high- but not low-molecular weight dna fragmentation ...	2006	16573736
the dual role of chorion peroxidase in bactrocera oleae chorion assembly.	in the present study, we reveal for the first time that the bactrocera oleae chorion peroxidase (bpxd) participates essentially in b. oleae chorion formation and clearly represents the homologous member of drosophila melanogaster chorion peroxidase (pxd). comparative sequence analysis disclosed that the bpxd cdna semi-central region, which encodes for the putative catalytic domain of the enzyme, exhibits great homology (98%) with its pxd counterpart. thus, it is very likely that bpxd is highly r ...	2006	16741869
geographical distribution and evolutionary history of organophosphate-resistant ace alleles in the olive fly (bactrocera oleae).	acetylcholinesterase (ace) is the molecular target of organophosphate (op) insecticides, and two mutations that confer different levels of op insensitivity have previously been identified in the olive fly, bactrocera oleae. numerous sensitive and two insensitive alleles (including one convergent acquisition) are described from the entire worldwide distribution of the fly. most of the variation is harbored in the native range of the species and in the middle east and consists of numerous low-freq ...	2006	16835025
autophagy is required for the degeneration of the ovarian follicular epithelium in higher diptera.	autophagy is a major pathway for the degradation of long-lived proteins and cytoplasmic organelles and an essential part of programmed cell death, as well. our findings indicate that programmed cell death of the ovarian follicle cells in the higher diptera species bactrocera oleae and ceratitis capitata manifests features of autophagic cell death. the follicle cells during the developmental stage 14 contain autophagic vacuoles and they do not exhibit caspase activity in any area of the egg chamb ...	2007	16921270
partial purification and insecticidal activity of toxic metabolites secreted by a mucor hiemalis strain (smu-21) against adults of bactrocera oleae and ceratitis capitata (diptera: tephritidae).	the secondary metabolites present in the methanol extract of a mucor hiemalis strain (smu-21) mycelia, cultured in liquid medium, were evaluated for toxicity to bactrocera oleae (gmelin) and ceratitis capitata (wiedemann) (diptera: tephritidae) adults. feeding and contact bioassays revealed that the methanol fraction of the crude supernatant was strongly toxic to both species. symptoms of toxicity developed quickly; lethargy occurred 1-2 h posttreatment, and mortality reached 82-97% after 24 h. ...	2006	17066796
organophosphate resistance in olive fruit fly, bactrocera oleae, populations in greece and cyprus.	the olive fruit fly bactrocera oleae (gmelin) (diptera: tephritidae) is the most important pest of olives in countries around the mediterranean basin. its control has been based mostly on bait sprays with organophosphate insecticides (usually dimethoate or fenthion) for about 40 years. in the present study, the resistance status of olive fruit fly populations to dimethoate was examined in greece and cyprus over 2 years. thirty-one populations from various regions of greece, nine from cyprus and ...	2007	17103369
olive fruit fly (diptera: tephritidae) populations in relation to region, trap type, season, and availability of fruit.	olive fruit fly, bactrocera oleae (gmelin), was monitored with adult captures by season and trap type, and was related to fruit volume and nonharvested fruit to elucidate the occurrence of the newly introduced pest in california. the highest numbers of adults captured in champ traps in olive trees, olea europaea, were in october in an inland valley location, and in september in a coastal location. comparisons of trap types showed that the number of olive fruit fly adults captured in pherocon am ...	2006	17195675
genetic drift and estimation of effective population size.	the statistical properties of the standardized variance of gene frequency changes (a quantity equivalent to wright's inbreeding coefficient) in a random mating population are studied, and new formulae for estimating the effective population size are developed. the accuracy of the formulae depends on the ratio of sample size to effective size, the number of generations involved (t), and the number of loci or alleles used. it is shown that the standardized variance approximately follows the chi(2) ...	1981	17249104
the transformer gene in bactrocera oleae: the genetic switch that determines its sex fate.	transformer (tra) is the second gene of a regulatory cascade based on rna splicing that determines sex in drosophila melanogaster. splicing of tra transcripts is regulated by the master gene sex lethal and tra itself regulates splicing of the transcriptional regulator doublesex (dsx). we present the isolation and characterization of botra, the olive fruit fly bactrocera oleae orthologue to the drosophila gene transformer. as in drosophila, botra transcripts are spliced in a sex-specific manner s ...	2007	17298554
probabilities for survival of glassy-winged sharpshooter and olive fruit fly pests in urban yard waste piles.	glassy-winged sharpshooter (homolodisca coagulate) and olive fruit fly (bactrocera oleae) were introduced into unturned, chipped yard waste piles to evaluate their survival with time and depth within the piles. in all three trials, no pests lasted more than 14 d, and in no trial did pests survive more than 4d at the 30 and 100 cm depths. no survivors were found after 14 d in any of the treatments at any depth. neither of the pests survived 100 cm after 2d. a mathematical model for describing pes ...	2008	17419052
the complete nucleotide sequence of the mitochondrial genome of the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae).	the complete mitochondrial genome of the oriental fruit fly bactrocera dorsalis s.s. has been sequenced, and is here described and compared with the homologous sequences of bactrocera oleae and ceratitis capitata. the genome is a circular molecule of 15,915 bp, and encodes the set of 37 genes generally found in animal mitochondrial genomes. the structure and organization of the molecule is typical and similar to the two closely related species b. oleae and c. capitata, although it presents an in ...	2007	17433576
psyttalia ponerophaga (hymenoptera: braconidae) as a potential biological control agent of olive fruit fly bactrocera oleae (diptera: tephritidae) in california.	the olive fruit fly, bactrocera oleae (rossi), is a newly invasive, significant threat to california's olive industry. as part of a classical biological control programme, psyttalia ponerophaga (silvestri) was imported to california from pakistan and evaluated in quarantine. biological parameters that would improve rearing and field-release protocols and permit comparisons to other olive fruit fly biological control agents were measured. potential barriers to the successful establishment of p. p ...	2007	17524155
survival and aging in the wild via residual demography.	information about the age distribution and survival of wild populations is of much interest in ecology and biodemography, but is hard to obtain. established schemes such as capture-recapture often are not feasible. in the proposed residual demography paradigm, individuals are randomly sampled from the wild population at unknown ages and the resulting captive cohort is reared out in the laboratory until death. under some basic assumptions one obtains a demographic convolution equation that involv ...	2007	17727909
evaluation of the impact on entomocoenosis of active agents allowed in organic olive farming against bactrocera oleae (gmelin, 1790).	several attempts for evaluating environmental impact of active agents allowed in organic olive farming against bactrocera oleae have been made, but very few studies were performed contemporaneously on more than one of them. insects were chosen as indicators because they are known to react very quickly to environmental perturbations, mainly at the community level. in fact, the coenosis is the functional unit interacting with biotic and abiotic environmental parameters. seven taxa, known for their ...	2015	17763034
assessment of susceptibility of olive cultivars to the bactrocera oleae (gmelin, 1790) and camarosporium dalmaticum (thüm.) zachos & tzav.-klon. attacks in calabria (southern italy).	within the framework of research concerning the application of techniques alternative to chemical pesticides for control of parasites, the c.r.a. experimental institute for olive growing for many years has been performing a large investigation in order to detect sources of genetic resistance in olive germplasm. in the present study we observed the behavior related to the olive fly (bactrocera oleae) infestation and camarosporium dalmaticum infection of ten olive cultivars farmed under the same a ...	2007	17763035
insecticidal activity of citrus aurantium fruit, leaf, and shoot extracts against adult olive fruit flies (diptera: tephritidae).	solvent extracts of differing polarity from citrus aurantium (l.) (rutaceae) fruit, leaves, and shoots were evaluated for biological activity against adults of the olive fruit fly, bactrocera oleae (gmelin) (diptera: tephritidae). using a petri dish residual exposure bioassay, we found that the petroleum ether extract from fruit alone showed insecticidal activity against the flies. the extract of the three fruit tissues (flavedo [peel], albedo, and flesh) indicated that bioactivity was limited t ...	2007	17849873
significance of chirality in pheromone science.	pheromones play important roles in chemical communication among organisms. various chiral and non-racemic pheromones have been identified since the late 1960s. their enantioselective syntheses could establish the absolute configuration of the naturally occurring pheromones and clarified the relationships between absolute configuration and bioactivity. for example, neither the (r)- nor (s)-enantiomer of sulcatol, the aggregation pheromone of an ambrosia beetle gnathotrichus sulcatus, is behaviora ...	2007	17855097
the gene transformer of anastrepha fruit flies (diptera, tephritidae) and its evolution in insects.	in the tephritids ceratitis capitata and bactrocera oleae, the gene transformer acts as the memory device for sex determination, via an auto-regulatory function; and functional tra protein is produced only in females. this paper investigates the evolution of the gene tra, which was characterised in twelve tephritid species belonging to the less extensively analysed genus anastrepha. our study provided the following major conclusions. firstly, the memory device mechanism used by this gene in sex ...	2007	18043746
alterations of the acetylcholinesterase enzyme in the oriental fruit fly bactrocera dorsalis are correlated with resistance to the organophosphate insecticide fenitrothion.	alterations of the structure and activity of the enzyme acetylcholinesterase (ache) leading to resistance to organophosphate insecticides have been examined in the oriental fruit fly, bactrocera dorsalis (hendel), an economic pest of great economic importance in the asia-pacific region. we used affinity chromatography to purify ache isoenzymes from heads of insects from lines showing the phenotypes of resistance and sensitivity to insecticide treatments. the ache enzyme from a strain selected fo ...	2008	18207076
determination of natural olive fruit fly pheromone in insect samples by enzyme linked immunoassays.	the olive fruit fly pheromone avidin-biotin elisa immunoassay, based on the use of polyclonal g antibodies derived from rabbits (reported previously) and a newer assay, based on the use of polyclonal y antibodies isolated from the eggs of laying hens (reported in this paper), were applied successfully for the analysis of natural pheromone in virgin adult female olive fruit flies. according to the results obtained, the pheromone content in the glands of adult female olive fruit flies increases fr ...	2008	18371673
efficacy of the pyrethroid alpha-cypermethrin against bactrocera oleae populations from greece, and improved diagnostic for an iache mutation.	the most important pest of olive orchards worldwide is the olive fruit fly bactrocera oleae (gmelin). its control in greece has been based on organophosphates (ops), but their intense use has led to the development of resistance. a test previously developed to monitor the trait may not be as robust as originally thought. the pyrethroid alpha-cypermethrin has recently been registered for bait sprays, as an alternative to ops.	2008	18381673