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relationship of bacillus subtilis clades associated with strains 168 and w23: a proposal for bacillus subtilis subsp. subtilis subsp. nov. and bacillus subtilis subsp. spizizenii subsp. nov.earlier phylogenetic studies based on the inferred dna sequences of the polc, rpob and gyra genes suggested that strains of the species bacillus subtilis formed two clusters, indicating the presence two closely related taxa; one contained the laboratory strain 168 and the other the laboratory strain w23. significant sexual isolation was found between strain 168 and members of the group containing w23, but no sexual isolation was observed between strain 168 and other members of the 168 group. dna ...199910425781
bacillus axarquiensis sp. nov. and bacillus malacitensis sp. nov., isolated from river-mouth sediments in southern spain.two gram-positive, rod-shaped, endospore-forming bacteria (strains cr-119(t) and cr-95(t)) were isolated from brackish sediments in the mouth of the river velez in malaga, southern spain, and subjected to a polyphasic taxonomic study. phenotypic tests showed that these strains were related to other bacillus species at a similarity level of less than 87.6 %. both strains are halotolerant, aerobic, chemoheterotrophic, motile with peritrichous flagella and biosurfactant producers. their endospores ...200515879268
in situ biosurfactant production by bacillus strains injected into a limestone petroleum reservoir.biosurfactant-mediated oil recovery may be an economic approach for recovery of significant amounts of oil entrapped in reservoirs, but evidence that biosurfactants can be produced in situ at concentrations needed to mobilize oil is lacking. we tested whether two bacillus strains that produce lipopeptide biosurfactants can metabolize and produce their biosurfactants in an oil reservoir. five wells that produce from the same viola limestone formation were used. two wells received an inoculum (a m ...200717172458
phylogeny and molecular taxonomy of the bacillus subtilis species complex and description of bacillus subtilis subsp. inaquosorum subsp. nov.the bacillus subtilis species complex is a tight assemblage of closely related species. for many years, it has been recognized that these species cannot be differentiated on the basis of phenotypic characteristics. recently, it has been shown that phylogenetic analysis of the 16s rrna gene also fails to differentiate species within the complex due to the highly conserved nature of the gene, yet dna-dna hybridization values fall well below 70 % for the same species comparisons. as a complementary ...200919622642
genome sequence of bacillus subtilis subsp. spizizenii gtp20b, isolated from the indian ocean.bacillus subtilis is an aerobic spore-forming gram-positive bacterium that is a model organism and of great industrial significance as the source of diverse novel functional molecules. here we present, to our knowledge, the first genome sequence of bacillus subtilis strain gtp20b isolated from the marine environment. a subset of candidate genes and gene clusters were identified, which are potentially involved in production of diverse functional molecules, like novel ribosomal and nonribosomal an ...201021183663
entianin, a novel subtilin-like lantibiotic from bacillus subtilis subsp. spizizenii dsm 15029t with high antimicrobial activity.lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. in this report, we used the previously reported subtilin autoinduction bioassay in combination with mass spectrometric analyses to identify the novel subtilin-like lantibiotic entianin from bacillus subtilis subsp. spizizenii ...201121239550
the genome sequence of bacillus subtilis subsp. spizizenii w23: insights into speciation within the b. subtilis complex and into the history of b. subtilis genetics.the genome sequence of bacillus subtilis subsp. spizizenii w23 has been determined. the sequence strongly suggests that w23 is a direct descendent of b. subtilis atcc 6633. w23 shares a 3.6 mb core genome with the intensively studied model organism b. subtilis subsp. subtilis 168, and gene order within this core has been strongly conserved. additionally, the w23 genome has 157 accessory (that is, non-core) genome segments that are not found in 168, while the 168 genome has 141 segments not found ...201121527469
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