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characterization of l-asparagine transport systems in stemphylium botryosum.l-asparagine uptake by stemphylium botryosum is mediated by two distinct energy- and temperature-dependent transport systems. one permease is relatively specific for l-asparagine and l-glutamine and is present in nutrient-sufficient mycelium. the specific permease shows an optimum ph at 5.2, saturation kinetics (km = 4.4 x 10(-4) m, vmax = 1.1 mumol/g per min), competitive gradient of l-asparagine, and higher affinity towards the l-isomer of asparagine. amide derivatives of l-asparagine (5-diazo ...19768427
primary interaction between antibody and components of alternaria. i. immunology and chemical characteristics of labeled antigens.components were isolated from alternaria tenuis and its culture filtrate, and were radiolabeled with 123-i. the labeled antigenic components had a high polysaccharide content as determined by staining patterns following electrophoresis in polyacrylamide gels and by inactivation with sodium metaperiodate. a primary binding test was employed to detect and measure serum antibodies to the components from a. tennuis. this procedure was more sensitive in detecting antibodies that bound to antigens tha ...197548526
atmospheric mold spores in tehran, iran.plate method, using modified sabouraud and littman oxgall agra media, was used to study atmospheric mold spores in tehran, iran, for a complete year. twenty-two different genera of molds were identified and counted. of these, yeast, penicillium, cladosporium, rhodotorula, alternaria, stemphylium and aspergillus were the most abundant and were seen all year 'round. other genera were observed seasonally and in small numbers.1978343658
incidence of fungal spores at the homes of allergic patients in an agricultural community. iii. associations with local crops.a predominantly agricultural community in california was surveyed for prevalent fungal spores during a 12-month period. alternaria, macrosporium and stemphylium were recovered during asparagus and strawberry harvesting times year-round. fusarium and botrytis were less frequently associated with the strawberry harvest and were recovered only during the first quarter of the year. epicoccum was recovered in the north end of the salinas valley in low numbers throughout the year and was strongly asso ...1979574745
[actinomycetes antagonistic to fungi and not affecting rhizobium meliloti].the effects of 481 actinomycetes isolated from agricultural soils supporting good growth of alfalfa or clover on two efficient strains of rhizobium meliloti a2 and s14 were studied. strain a2 was inhibited by 28% of the isolates and strain s14 was inhibited by 31% of them. no significant difference was found between the resistance of both actinomycete strains. the effects of the 288 isolates not affecting r. meliloti on six fungi were also studied. the most sensitive fungus was stemphylium sarci ...1978657008
a study on fungal radioresistance and sensitivity.lower doses of gamma and x-rays (5 and 1 krad, respectively) stimulated the spore germination and growth of 7 tested fungi. higher doses (500 and 100 krad, respectively) revealed that epicoccum nigrum was the most radioresistant, while alternaria humicola, stemphylium verruculosum, rhizopus nigricans, and cladosporium herbarum were of moderate resistance. aspergillus niger and penicillium oxalicum were the most radiosensitive. protein and polysaccharide synthesis were inhibited in the mycelium d ...1977857505
ultrastructure and lipid identification during conidium germination of stemphylium sarcinaeforme.multicelled conidia of stemphylium sarcinaeforme germinate in water forming several germ tubes. individual cells within conidia are connected by pores which are plugged in ungerminated conidia and open in germinated ones. during germination, vacuoles enlarge, endoplasmic reticulum profiles increase in number, and mitochondria change from spherical to elongate. the germ tube wall is laid down at the site of emergence from the conidium. shortly after germination, a septum with a central pore forms ...1976943218
possible role of solar radiation on the viability of some air fungi in egypt.the high occurrence of alternaria humicola and the moderate occurrence of stemphylium verruculosum, rhizopus nigricans, and epicoccum nigrum in the air of egypt could be correlated to their apparent resistance to solar radiation. cladosporium herbarum, aspergillus niger, and penicillium oxalicum, although highly occurring, were light-sensitive. the miniumum solar radiation in november, coupled with the highest total fungal catch, dominated by cladosporium herbarum, reveals the importance of sola ...19761037050
new leaf diseases of barley in egypt.leaf diseases of barley were observed also in egypt. from leaves of barley were isolated: helminthosporium teres, h. gramineum, stemphylium vesicarium, alternaria triticina, vlocladium chartarum, acnemonium kiliense, stemphylium spec. accompanied with the pleospora stage. inoculations on both attached and detached leaves showed that all the tested fungi were pathogenic, except acremonium kiliense and ulocladium chartarum.19761037183
primary interaction between antibody and components of alternaria. ii. antibodies in sera from normal, allergic, and immunoglobulin-deficient children.antibodies in sera from normal, allergic, and immunoglobulin-deficient children were studied for binding to radiolabeled components of alternaria tenuis. significant binding levels were found in 103 of 105 sera from normal children. the levels were age-dependent, rising from a low point in the 7- to 12-month age group to adult levels by the age of 8 years. levels of binding to two antigens, a culture filtrate derivative (125i-clf) and a mycelial derivative (125-i-hs), were similar. sera from ast ...19751094055
[microflora of the atmosphere in the city of trujillo (peru). iii. genera isolated during the year 1971].there were 1762 fungi isolated from the atmosphere of trujillo city (peru) during the year 1971. these isolants belonged to 23 genera of fungi. the most frequently isolated were: cladosporium, penicillium, fusarium, stemphylium, alternaria, helminthosporium, aspergillus and tilachlidium. nine genera are reported for first time from the air of the city (tilachlidium, cephalosporium, mucor, aureobasidium, hormiscium, trichoderma, stachybotrys, paecilomyces and fisidium). six genera may be alergeni ...19751152971
a study of the aeromycoflora of cádiz: relationship to anthropogenic activity.we describe a quantitative and qualitative study of the fungal spores found in the air of cádiz during 1989 using a cour-type trap. the results of this study can be extrapolated to other coastal cities of southern europe with a mediterranean climate. the spores identified have been classified into 25 taxonomic categories. the most abundant were cladosporium, chaetomium and ustilago, and the most frequent, in addition to those mentioned, were alternaria, ascophyta and venturia. the great abundanc ...19921342902
mutagenicity of stemphyltoxin iii, a metabolite of alternaria alternata.some common decay organisms of vegetables and ripened fruits are alternaria species. even fruits and vegetables kept under refrigeration can be spoiled by alternaria species because the mold grows at low temperatures. alternaria alternata is commonly found in grain in areas with a high incidence of esophageal cancer. three metabolites, altertoxins i, ii, and iii, have been isolated from a. alternata and have hydroxyperylenequinone structures. although other perylenequinone metabolites such as st ...19912036005
use of the extended phadebas rast panel in the diagnosis of mould allergy in asthmatic children.the clinical significance of mould allergens in phadebas rast panel was investigated in 121 asthmatic children. they were selected from a total population of 1649 patients. the patients were distributed into four groups, based on the combination of positive or negative skin prick tests (spt) together with symptoms suggestive or not of mould sensitivity. mould-specific ige antibodies were investigated using the original rast panel (alternaria, aspergillus, candida, cladosporium, mucor and penicil ...19912039083
stemphyltoxin iii from alternaria alternata.stemphyltoxin iii, (6ar*, 6bs*, 7r*, 8r*)-3,6a, 10-trihydroxy-4,9-dioxo-4,6a,6b,7,8,9-hexahydro-7,8-epoxyperylene, a known metabolite of stemphylium botryosum var. lactucum, has been identified as a mutagenic metabolite of alternaria alternata by spectroscopic studies. the 13c-nmr spectral data, which were not reported previously, are presented.19892746264
[microorganisms growing on the inner surfaces of prefabricated houses and their control].microorganisms which isolated from the plasters of prefabricated houses, constructed by yübetaş company in oran ankara were examined. as a result of isolations, alternaria, penicillium and stemphylium species of fungi were founded on the plasters. the growth of the fungi was tested on agrisan megess and dyo sadosan make plastic wall paints and it was found that these fungi showed a profuse growth in the former make paint, but not in the second one. when mancozeb (dithan-m-45) in 0.2% and copper ...19873441227
[does immediate-type respiratory allergy occur regarding stemphylium? evaluation of 39 challenge tests].does immediate respiratory allergy with stemphylium exist? about 39 provocation tests. we present 39 children with positive cutaneous and/or rast tests for stemphylium mold. provocation tests individualize 19 patients (14 boys and 4 girls, 10 to 16 years old in 17/19) with a respiratory sensitivity: 6/10 by passive anterior rhinomanometry and 13/15 by bronchial tests. clinical symptoms are asthma and spasmodic rhinitis (14/19), predominantly from june to august. we noted a mite allergy in 11 cas ...19863453724
senescence and death of the conidiogenous cell in stemphylium botryosum wallroth. 19734360416
progesterone transformations as a diagnostic feature in the genera alternaria, stemphylium and cladosporium. 19744435656
a fine-structural study of conidium initiation in stemphylium botryosum wallroth. 19724561137
surface structure of the conidium and conidiophore of stemphylium botryosum. 19734572484
cyanide degradion by an enzyme from stemphylium loti. 19725065258
[quincke's edema due to alternaria and stemphylium]. 19715103706
comparative skin tests with two stemphylium species. 19715107225
metabolic products of stemphylium radicinum. iv. minor products. 19715170055
specificity of immunofluorescent staining for study of aspergillus flavus in soil.fluorescein-labeled antiserum prepared with aspergillus flavus strain cs was tested for specificity by staining fungi grown in soil in the vicinity of buried slides. all 14 strains of a. flavus fluoresced as intensely or nearly as intensely as the antigen control. among 21 isolates of species of aspergillus other than a. flavus, 17 reacted with moderate to low fluorescence at intensities readily distinguishable from that of a. flavus. the fluorescence of the remaining four cultures, and particul ...19655325934
pectic and cellulolytic enzymes of stemphylium radicinum. 19695392642
comparative abilities of stemphylium botryosum and helminthosporium turcicum to induce and degrade a phytoalexin from alfalfa. 19695392960
degradation of alfalfa phytoalexin by stemphylium botryosum. 19695392961
[the effect of common leaf spot (pseudopeziza medicaginis (lib.) sacc.) and leaf spot (stemphylium botryosum wallr.; phoma medicaginis malbr. et roum.) on the co2 assimilation by the leaves of alfalfa (medicago sativa l.)]. 19695396112
setlow rb: ergosterol and substitutes for the ultraviolet radiation requirement for conidia formation in stemphylium solani. 19685689477
studies on the carbon, nitrogen, and vitamin nutrition of stemphylium radicinum. 19685689921
stemphone, a biologically active yellow pigment produced by stemphylium sarcinaeforme (cav.) wiltshire. 19685693064
[stability of pigmented fungi stemphylium botryosum wallr and cladosporium cladosporivides (freis) de vries towards gamma-irradiation]. 19685753849
perfect states of stemphylium. 19695814421
a new species of stemphylium. 19655896240
a new species of stemphylium on chrysanthemum cinerariaefolium vis. 19666008763
typification of alternaria, stemphylium, and ulocladium. 19676068270
ergosterol and the ultraviolet requirement for production of conidia in stemphylium solani. 19676068731
subcutaneous phaeohyphomycosis (stemphylium sp and cladosporium sp infections) in a cat. 19846092316
shared allergenic and antigenic determinants in alternaria and stemphylium extracts.alternaria and stemphylium extracts were compared by means of various immunochemical and biologic methods. skin-test responses to alternaria and stemphylium in allergic patients were positively correlated, and rast binding values with the sera of 30 patients utilizing solid-phase alt-i, alternaria (70% ammonium sulfate--precipitable fraction), and stemphylium allergens showed significant correlation, giving evidence for the presence of common allergenic determinants among these three extracts. i ...19826183306
[bronchopulmonary pathology with hypereosinophilia of fungal origin (excluding allergic bronchopulmonary aspergillosis)].five cases of eosinophil lung are reported in which the fungus responsible for the affection was not aspergillus. documented data include reports on 19 similar cases with a clinical picture suggestive of allergic bronchopulmonary aspergillosis but with negative tests for aspergillus. the various fungal species isolated included candida albicans, penicillium, geotrichum candidum, stemphylium lanuginosum, culvularia lunata, and drechsleria hawaïensis. diagnostic criteria are discussed, with partic ...19836878115
allergic bronchopulmonary stemphyliosis.a patient is described in whom a clinical picture resembling allergic bronchopulmonary aspergillosis was found to be caused by hypersensitivity to the fungus stemphylium lanuginosum. bronchopulmonary reactions to antigens other than aspergillus may be more frequent than is currently believed.19807192017
[molds in foods of the czechoslovak socialistic republik (author's transl)].the mycoflora of 13.527 g of food powders which were normal upon sensory evaluation as well of 22.017 pieces of surface sterilized grains and nuts was examined in the czechoslovak socialist republic. 147.027 colonies of 44 genera of molds were isolated and identified from samples. colonies of penicllium sp., aspergillus sp., and cladosporium sp. occured most often, less often or quite infrequently rhizopus sp., mucor sp., absidia sp., alternaria sp., circinella sp., trichoderma sp., botrytis sp. ...19817269803
production of a toxin stemphol by stemphylium species.five of 11 isolates of stemphylium botryosum wallr. from oilseed rape produced the phytotoxin stemphol when cultured on rice, with yields varying from 15.0 to 98.4 mg/kg, and three of them also produced the toxin on czapek dox (cd) liquid medium supplemented with cations (0.12-0.31 mg/l) and potato-dextrose (pd) broth (0.37-1.47 mg/l). in liquid cultures about 95% of stemphol was found in the mycelium, and toxin production was slightly increased when cultures were exposed to near uv light rather ...19948032689
further studies on egyptian soil fungi: succession of sugar and osmophilic fungi in soil amended with five organic substrates.the sugar and osmophilic fungal composition of soils amended with five organic substrates (newspaper, orange peel, bromegrass leaves, wheat straw and wood sawdust) was estimated after 2, 4, 6, 8 and 10 weeks using the dilution plate method on glucose and 50% sucrose czapek's agar media. wheat straw was the best substrate for total counts of both sugar and osmophilic fungi followed by newspaper, bromegrass leaves, wood sawdust and orange peel. wood sawdust supported the highest average counts of ...19969144956
occurrence of itraconazole-tolerant micromycetes in the soil and food products.unexpected pathogens from the environment represent considerable risk for humans with impaired health. we examined the occurrence of itraconazole tolerant micromycetes in soil and in maize products. five concentrations of itraconazole (2.5-12.5 micrograms/ml) selected according to known treatment schedules for human patients were incorporated into sabouraud agar with chloramphenicol and rose bengal and diluted samples were inoculated onto the agar surface. after 7-d growth at 22 degrees c coloni ...199911097027
eric and rep-pcr banding patterns and sequence analysis of the internal transcribed spacer of rdna of stemphylium solani isolates from cotton.the genetic diversity of the stemphylium solani isolates from cotton was assessed by enterobacterial repetitive intergenic consensus (eric) and repetitive extragenic palindromes (rep)-pcr fingerprinting. twenty eight monosporic isolates of s. solani from cotton were used along with five isolates from tomato and one isolate of alternaria macrospora from cotton for comparison. the dendrogram obtained revealed clear differences between the cotton and tomato isolates as well as between the tomato is ...200211927982
eric- and rep-pcr amplify non-repetitive fragments from the genome of drechslera avenae and stemphylium solani.in the present study, the homology relationships among fragments amplified by eric (enterobacterial repetitive intergenic consensus) and rep (repetitive extragenic palindromic) primers in drechslera avenae and stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were investigated by southern hybridization followed by dna sequencing. eric- and rep-pcr were performed for 24 isolates of d. avenae and 33 isolates of s. solani. the banding patterns ob ...200212052550
allergen detection from 11 fungal species before and after germination.allergens dispersed by airborne fungal spores play an important but poorly understood role in the underlying cause and exacerbation of asthma. previous studies suggest that spores of alternaria and aspergillus release greater quantities of allergen after germination than before germination. it is unknown whether this is true of other allergenic fungi.200312589346
a sensitive two-site enzyme-linked immunosorbent assay for measurement of the major alternaria alternata allergen alt a 1.alt a 1 is the major allergen in alternaria alternata, one of the most important fungi associated with allergic diseases. mold allergenic extracts show considerable heterogeneity, and thus accurate standardization of these extracts is essential to guarantee their quality.200312775134
airborne fungal spores at the belgrad forest near the city of istanbul (turkey) in the year 2001 and their relation to allergic diseases.the aim of this study was to investigate the relationship between airborne fungal spores and allergic diseases; for this reason, the airborne fungal spores that were obtained from five different locations of belgrad forest were isolated, determined and studied quantitatively. totally 120 samples were examined by using the petri plate gravitational method, and fungi obtained from these samples were isolated and 600 colonies were counted. by identification of these isolations, 13 genera (mucor, rh ...200312964180
radicinin: a new pigment from stemphylium radicinum. 195313081157
radicinin: a metabolite from stemphylium radicinum. i. chemistry and action. 195513269178
radicinin: a metabolite from stemphylium radicinum. ii. on the mechanism of its biosynthesis. 195513269179
patterns of mold sensitivity in the subtropical gulf coast.we sought to determine the patterns of mold- and nonmold positive skin tests in a subtropical gulf coast climate and to determine whether any particular positive skin tests predict "allergic versus nonallergic" status as shown on the full panel of skin testing.200415054371
allergenic fungi spore records (15 years) and sensitization in patients with respiratory allergy in thessaloniki-greece.the prevalence of respiratory allergy to fungi spores (fs) is not precisely known but is estimated at 20-30% of atopic patients. there are no aerobiological records, necessary for respiratory allergy diagnosis and treatment, in thessaloniki and generally in greece.200415552717
fungal spores in the atmosphere of rzeszów (south-east poland).continuous aerobiological monitoring has been conducted in rzeszów from 2000-2002, using the volumetric method. on each microscope slide 1 horizontal band was analyzed, divided into 24 fields corresponding to hours. for the analysis, 10 easy to identify fungal spores were chosen from ones regarded as allergenic: alternaria, botrytis, cladosporium, drechslera (type), epiccocum, ganoderma, pithomyces, polythrincium, stemphylium, and torula. the results were statistically tested using the chi2 test ...200415627338
atmospheric concentrations of cladosporium spp. and alternaria spp. spores in zagreb (croatia) and effects of some meteorological factors.the aim of the study was to analyse the relationship between meteorological conditions and alternaria and cladosporium spore concentrations in the air of zagreb in august 2002 and august 2003. these months were chosen because they represented climatic extremes. a 7-day vpps 2000 hirst volumetric pollen and spore trap was used for spore sampling. spores marked as 'others' (ascospores, basidiospores, epicoccum, ustilago, pithomyces, helminthosporium, stemphylium, torula, botrytis, didymella) were ...200415627341
natural occurrence of fungi and fungal metabolites in moldy tomatoes.fresh tomatoes, homegrown and from supermarkets, with developing fungal lesions were collected. each lesion was sampled, and the resulting fungal cultures were identified morphologically and extracted for analyzes of secondary metabolites. the tomatoes were incubated at 25 degrees c for a week, extracted, and analyzed for fungal metabolites. extracts from pure cultures were compared with extracts from the moldy tomatoes and fungal metabolite standards in two hplc systems with dad and fld detecti ...200415675796
restriction mapping of the igs region in alternaria spp. reveals variable and conserved domains.accurate identification of alternaria spp. is dependent upon the production of diagnostic morphological characters under defined cultural conditions and the proper assessment of character variation. this process is often compromised by variation in laboratory facilities and technical expertise. to assist taxon identification and phylogenetic studies, restriction site information from the intergenic spacer (igs) region of nuclear rdna was evaluated. restriction maps were constructed from 15 speci ...200515736866
lateral transfer of mating system in stemphylium.the fungal genus stemphylium (ascomycota) contains selfing species that evolved from outcrossing ancestors. to find out how selfing originated, we analyzed the stemphylium mat loci that regulate sexual reproduction in ascomycetes and compared mat structures and phylogeny with a multigene stemphylium species phylogeny. we found that some stemphylium species' mat loci contained a single gene, either mat1-1 or mat1-2, whereas others contained a unique fusion of the mat1-1 and mat1-2 regions. in all ...200516055562
the major allergen of alternaria alternata (alt a 1) is expressed in other members of the pleosporaceae family.there is general consensus regarding the scarce cross-reactivity existing between alternaria alternata and other allergenic moulds such as aspergillus fumigatus, penicillium notatum or cladosporium herbarum. however, a. alternata has been shown to have a very significant level of allergenic cross-reactivity with other fungi belonging to the pleosporaceae family. to date, no biological identity or homologies with other proteins have been described for alt a 1, and it remains unclear whether the m ...200616466440
infection rates of perennial ryegrass seeds (lolium perenne l.) with different fungi.authors made investigations to map occurrence of different fungal pathogens on seeds of varieties from different grass species. this work presents data on three cultivars of perennial ryegrass (lolium perenne l.). when seeds were germinated, eight genuses of fungi were identified belonging to the deuteromycota. alternaria was the most frequently occurred genus. drechslera, bipolaris, cladosporium, stemphylium, and epicoccum spp. has also been found in the seed samples. two genus of fungal pathog ...200516637199
isolation and characterization of stemphylin, a chromone glucoside from stemphylium botryosum.a phytotoxic compound was isolated from a liquid culture medium of stemphylium botryosum, a pathogen of lettuce. the toxin is an amorphous yellow solid with absorbance maxima at 218, 268, and 427.5 nm and exhibits a bathochromic shift in alkaline ph. it has a molecular weight of 370 and an empirical formula of c(17)h(22)o(9). glucose and aromatic pigments are detected after acid hydrolysis. based on its spectral and chemical properties, the proposed structure of the toxin is 3-hydroxy- 2,2-dimet ...197516659141
contribution of a cyanide-insensitive alternate respiratory system to increases in formamide hydro-lyase activity and to growth in stemphylium loti in vitro.stemphylium loti, a pathogen of a cyanogenic plant, possesses a cyanide-insensitive alternate respiratory pathway. in the absence of cytochrome inhibitors, the alternate system had only a minor role in respiration. when s. loti was grown in medium amended with antimycin to block the cytochrome chain, the alternate system accounted for the total oxygen consumption associated with respiration.the contribution of the alternate respiratory system to increases in formamide hydro-lyase (fhl) activity ...197716660201
a novel enolic beta-ketoaldehyde phytotoxin produced by stemphylium botryosum f. sp. lycopersici : partial chemical and biological characterization.a new phytotoxin, stemphyloxin i, c(21)h(32)o(5), was isolated from cultures of the pathogenic fungus stemphylium botryosum f. sp. lycopersici. the toxin is a tricyclic compound possessing a most unusual beta-ketoaldehyde group. injection of stemphyloxin i into a tomato leaflet caused unlimited necrotic spots and a loss of turgor, which at higher toxin concentration wilted the whole compound leaf. visible symptoms could be observed at a toxin concentration as low as 2.7 micromolar. stemphyloxin ...198216662165
the family pleosporaceae: intergeneric relationships and phylogenetic perspectives based on sequence analyses of partial 28s rdna.the pleosporaceae is an important loculoascomycete family. there has been disagreement, however, regarding the taxonomic placement of many genera within this family. this study investigates phylogenetic relationships among the genera cochliobolus, kirschsteiniothelia, leptosphaerulina, macroventuria, pleospora, pyrenophora, and wettsteinina. partial 28s rdna sequences from taxa within these genera were analyzed with maximum parsimony, likelihood and bayesian methods. cochliobolus can be segregat ...200617139850
brown spot control on pear: infection models versus the inoculum pressure in belgium.stemphylium conidia and pleospora ascospores were monitored in pear orchards in the region of haspengouw in belgium during the infectious periods of brown spot disease (end may - end august) in the years 2002, 2004 and 2005. the seasonal and daily dynamics of the captured fungal spores are discussed and a correlation analysis was performed to determine possible correlation with weather parameters. furthermore the fungicide cover obtained by spraying upon climatological infection risk is compared ...200617390829
crystallization and x-ray analysis of stemphyloxin i, a phytotoxin from stemphylium botryosum.certain isolates of the plant-pathogenic fungus stemphylium botryosum produce a phytotoxin, stemphyloxin i. this toxin (c(21)h(34)o(6)) was crystallized and its structure was determined by x-ray crystallography to be a beta-ketoaldehyde trans-decalin. this compound is a highly unusual natural product. iron (fe(3+)) controls production of toxin by this fungus. furthermore, iron reacts with the toxin to yield a colored product which aids in its detection on chromatograms and in its quantitative es ...198317754554
fungitoxicity of lyophilized and spray-dried garlic extracts.among the compounds discussed for anti-microbial and anti-fungal use allicin (allylthiosulfinate, diallyl disulfide-s-monoxide), an active ingredient of garlic, has attracted considerable attention. the objective of this study is to determine the antifungal activity of a local garlic ecotype (voghiera) extracts against different pathogens. primary screening was carried out by the agar plates technique using ethanol garlic extract at four final concentrations against the following organisms: alte ...200717763036
antimicrobial activity and the major components of the essential oil of plectranthus cylindraceus.the traditional uses of the aerial parts of fresh plectranthus cylindraceus by the dhofaris in oman were investigated on the basis of antimicrobial properties and composition of its herb oil.200717897226
analytical bias of cross-reactive polyclonal antibodies for environmental immunoassays of alternaria alternata.alternaria alternata is recognized as an important aeroallergen indoors and outdoors, and exposure to the fungus has been identified as a risk factor for asthma. two recent publications concluded that 95% to 99% of american homes contained detectable amounts of alternaria antigens when analyzed with a polyclonal antibody (pab)-based elisa.200818036643
airborne fungi in child day care centers in edirne city, turkey.the purpose of this study was to determine the concentration, in terms of monthly and seasonal distribution and in relation to meteorological factors, of indoor and outdoor microfungi at selected sites in several child day care centers in the city of edirne, turkey. samples were collected at one month intervals over a period of 12 months between january-december 2004, by exposing petri plates containing peptone dextrose agar with rose-bengal and streptomycin medium to the air for 10-15 min. a to ...200818264791
first occurrence of strobilurin-resistant isolates of stemphylium vesicarium in an italian pear orchard. 200718396802
alternaria measures in inner-city, low-income housing by immunoassay and culture-based analysis.sensitivity to alternaria allergens has been associated with severe asthma and life-threatening exacerbations, and a high prevalence of alternaria sensitivity has been reported among inner-city populations. traditionally, epidemiologic studies have measured indoor alternaria concentrations by cultural analyses; however, the number of viable spores may not be a good proxy for allergen levels. furthermore, other genera share epitopes with alternaria that may contribute to the allergenic effect.200818450123
[contribution to the aeromycological study in the atmosphere of the city of granada (south of spain): seasonal and daily variations.].the seasonal and daily variations in the concentrations of certain fungal spores in the atmosphere of the city of granada (south of spain) have been studied. sampling was carried out in 1994 using a hirst-type volumetric spore trap. the spores analysed correspond to the genera alternaria, cladosporium (cladosporium cladosporioides and cladosporium herbarum), fusarium, stemphylium and ustilago. the seasonal-variation data obtained indicate that most of these spores are abundant throughout the yea ...199918473554
effect of relative humidity and interrupted wetness periods on brown spot severity of pear caused by stemphylium vesicarium.abstract field observations in four pear orchards during 5 years from april to october indicated that days with uninterrupted wetness of variable length represented 83.9% of the total days studied. however, days with surface wetness interruptions and with high relative humidity (rh) (>/=90%) without wetness occurred with a frequency of 7.1 and 6.2%, respectively. accordingly, the effect of interruption of 24-h wetness periods by dry periods of high or low rh on infections caused by stemphylium v ...200218944145
purification and biological characterization of host-specific sv-toxins from stemphylium vesicarium causing brown spot of european pear.abstract culture filtrates of a pathogenic isolate (it37) of stemphylium vesicarium, causing brown spot of european pear, induced veinal necrosis only on pear leaves susceptible to the pathogen. two host-specific toxins, sv-toxins i and ii, were purified from culture filtrates of it37 by successively using amberlite xad-2 resin adsorption, cellulose thin-layer chromatography, and high-performance liquid chromatography under three different sets of conditions. susceptible cultivars showed veinal ...199918944740
fungal sensitivity to and enzymatic degradation of the phytoanticipin alpha-tomatine.abstract alpha-tomatine, synthesized by lycopersicon and some solanum species, is toxic to a broad range of fungi, presumably because it binds to 3beta-hydroxy sterols in fungal membranes. several fungal pathogens of tomato have previously been shown to be tolerant of this glycoalkaloid and to possess enzymes thought to be involved in its detoxification. in the current study, 23 fungal strains were examined for their ability to degrade alpha-tomatine and for their sensitivity to this compound an ...199818944982
the effects of meteorological factors on airborne fungal spore concentration in two areas differing in urbanisation level.although fungal spores are an ever-present component of the atmosphere throughout the year, their concentration oscillates widely. this work aims to establish correlations between fungal spore concentrations in porto and amares and meteorological data. the seasonal distribution of fungal spores was studied continuously (2005-2007) using volumetric spore traps. to determine the effect of meteorological factors (temperature, relative humidity and rainfall) on spore concentration, the spearman rank ...200919048306
bioactive metabolites from the endophytic fungus stemphylium globuliferum isolated from mentha pulegium.the endophytic fungus stemphylium globuliferum was isolated from stem tissues of the moroccan medicinal plant mentha pulegium. extracts of the fungus, which was grown on solid rice medium, exhibited considerable cytotoxicity when tested in vitro against l5178y cells. chemical investigation yielded five new secondary metabolites, alterporriol g (4) and its atropisomer alterporriol h (5), altersolanol k (11), altersolanol l (12), stemphypyrone (13), and the known compounds 6-o-methylalaternin (1), ...200919271717
seasonal changes in airborne fungi and bacteria at a dairy cattle concentrated animal feeding operation in the southwest united states.the objective of this study was to evaluate a dairy located in the arid southwest united states to determine the concentrations and seasonal variation of airborne fungi and bacteria and to determine the percentage of antibiotic resistant staphylococcus aureus. the authors used two-stage ambient air sampling systems to measure the culturable airborne fungal organisms and bacteria on a monthly basis. the authors recovered the most fungal, bacterial, and s. aureus organisms during the spring months ...200919452834
pleospora species with stemphylium anamorphs: a four locus phylogeny resolves new lineages yet does not distinguish among species in the pleospora herbarum clade.stemphylium is a genus of plant pathogens and saprobes in the pleosporaceae (pleosporales, dothideomycetes, ascomycetes). the teleomorphs of stemphylium, where known, are in pleospora, with pleospora herbarum as the type. the goal of this study was to present a rigorous phylogenetic analysis of the relationships among stemphylium isolates with particular emphasis on species delimitation in the p. herbarum clade, on possible new species and on the relationship of clades to cultures from type spec ...200919537206
molecular cloning, functional analysis and localization of a novel gene encoding polygalacturonase-inhibiting protein in chorispora bungeana.polygalacturonase-inhibiting proteins (pgips) are plant defense proteins. to date, no spatial distribution of pgips and interaction between pgips and nitric oxide (no) in plant were described. here, we first reported the full-length cdna sequence of pgip of chorispora bungeana (cbpgip1). notably, immunofluorescence localization showed that the cbpgip was evenly distributed in leaves but it was mainly localized in epidermis and vascular bundle in stems and roots. further studies indicated that cb ...200919885675
quantitative detection of pear-pathogenic stemphylium vesicarium in orchards.abstract isolates of stemphylium vesicarium causing brown spot of pear can be distinguished from nonpathogenic isolates of s. vesicarium from pear or from other hosts on the basis of distinctive amplified fragment length polymorphism fingerprinting profiles. dna fragments specific for isolates pathogenic to pear were identified and a quantitative polymerase chain reaction (pcr) was developed on the sequence from one of these specific dna loci. this taqman pcr has a high sensitivity with a dynami ...200919900004
baseline sensitivity of stemphylium vesicarium, the causal agent of pear brown spot, to boscalid. 200920222565
outdoor allergenic fungal spores: comparison between an urban and a rural area in northern portugal.the frequency and concentration of many airborne fungal spores associated with respiratory allergy symptoms are influenced by geographical and climatic characteristics.201020461966
molecular and morphological description of two new species of stemphylium from china and france.two new species of stemphylium (anamorphic pleospora) are described on the basis of morphological characters and molecular phylogenetic analyses. stemphylium phaseolina and s. variabilis were isolated respectively from diseased leaves of phaseolus vulgaris l. in hebei province, china, and from diseased leaves of allium sativum l. in angres, france. the two species exhibit characteristic stemphylium morphology but are distinct from similar species based on the morphology and development of conidi ...201020524602
a thaumatin-like gene from asparagus officinalis (aoprt-l) exhibits slow activation following tissue maceration or salicylic acid treatment, suggesting convergent defence-related signalling in monocots.summary messenger rna derived from mechanically separated cells of asparagus has proved to be an enriched source of defence-related transcripts. we describe the characterization of a novel pr-5 gene coding for a secreted protein of neutral pi (aoprt-l) that is strongly up-regulated following cell isolation or following accelerated tissue ageing caused by tissue maceration, but which is also responsive to salicylic acid, a defence-related signal not normally associated with wound responses. infec ...200020572983
molecular characterization of embellisia and nimbya species and their relationship to alternaria, ulocladium and stemphylium.dna sequences from rdna and protein-coding regions were determined for six embellisia and two nimbya spp. and were compared to those from alternaria, ulocladium and stemphylium spp. sequences determined included rdna from the nuclear internal transcribed-spacer region (its1/5.8s/its2) and the mitochondrial small-subunit (mt ssu) and a portion of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene. phylogenetic analyses were performed on each dataset separately and then combined for total evi ...200321149017
phylogeny of stemphylium spp. based on its and glyceraldehyde-3-phosphate dehydrogenase gene sequences.the phylogenetic relationships among 44 isolates representing 16 species of stemphylium were inferred from its and glyceraldehyde-3-phosphate dehydrogenase (gpd) sequence data. the results generally agree with morphological species concepts. there was strong support for monophyly of the genus stemphylium. analysis of the gpd fragment in particular was useful for establishing well-supported relationships among the species and isolates of stemphylium. species of stemphylium that appear to have los ...200221156539
sinomyces: a new genus of anamorphic pleosporaceae.sinomyces gen. nov. is described for ulocladium alternariae and two new species from china. these three species differ from ulocladium in producing simple conidiophores with a single, cicatrized apical pore or 1 or 2 short, uniperforate, geniculate sympodial proliferations. phylogenetic analysis of partial dna sequences of the glyceraldehyde-3-phosphate dehydrogenase gene (gpd) gene and a fragment of the alternaria alternata major allergen (alt a 1) gene, using maximum-parsimony (mp), maximum-li ...201021315316
fungal spores from pleosporales in the atmosphere of urban and rural locations in portugal.fungal spores are a significant fraction of the atmospheric bioparticles (bioaerosols) and many species are capable of inducing the production of specific immunoglobulin e (ige), aggravating the clinical symptoms of allergic respiratory diseases in sensitized individuals. the aim of this work was to evaluate the distribution of potentially allergenic pleosporales spores in two locations with different urbanization indexes, characterizing its seasonal pattern. the seasonal distribution of several ...201021491687
identification of allergens homologous to alt a 1 from stemphylium botryosum and ulocladium botrytis.several studies have demonstrated that proteins homologous to the alt a 1 major allergen of alternaria alternata are expressed in other members of the pleosporaceae family. however, since no direct biochemical data have been reported concerning the presence of alt a 1 allergen homologues capable of binding ige in the excretion-secretion products of stemphylium and ulocladium, our objective was to explore their presence in stemphylium botryosum and ulocladium botrytis. s. botryosum and u. botryti ...201121504377
mycological survey of activated sludge in mbrs.the objective of this research was to conduct a survey of fungi in activated sludge plants with membrane bioreactors (mbrs). thirty-six samples of both aerobic and anoxic activated sludge were taken from two plants with mbrs treating domestic wastewater. over a period of 8 months, two samples from each plant were taken per month. the samples were prepared for count and identification of fungi. the obtained data show that 61 species belonging to 30 genera were identified from activated sludge sam ...201121554420
contrasting codon usage patterns and purifying selection at the mating locus in putatively asexual alternaria fungal species.sexual reproduction in heterothallic ascomycete fungi is controlled by a single mating-type locus called mat1 with two alternate alleles or idiomorphs, mat1-1 and mat1-2. these alleles lack sequence similarity and encode different transcriptional regulators. a large number of phytopathogenic fungi including alternaria spp. are considered asexual, yet still carry expressed mat1 genes. the molecular evolution of alternaria mat1 was explored using nucleotide diversity, nonsynonymous vs. synonymous ...201121625561
susceptibility of clover species to fungal infection: the interaction of leaf surface traits and environment.many foliar pathogens require free water to germinate; therefore, disease pressure should favor plants that are able to repel water. for a suite of 18 sympatric clover species (trifolium and medicago, fabaceae), we evaluated leaf traits affecting leaf wetness and susceptibility to infection by the fungal pathogen stemphylium sp., causal agent of stemphylium leaf spot. spore germination increased with time in free water, and the relative susceptibility of host plants to infection was proportional ...200321659180
bivalent transition metal complexes of o-hydroxyacetophenone [n-(3-hydroxy-2-naphthoyl)] hydrazone: spectroscopic, antibacterial, antifungal activity and thermogravimetric studies.schiff base complexes of cu(ii), ni(ii) and zn(ii) with the o-hydroxyacetophenone [n-(3-hydroxy-2-naphthoyl)] hydrazone (h(2)o-hahnh) containing n and o donor sites have been synthesized. both ligand and its metal complexes were characterized by different physicochemical methods, elemental analysis, molar conductivity ((1)h nmr, (13)c nmr, ir, uv-visible, esr, ms spectra) and also thermal analysis (tg and dtg) techniques. the discussion of the outcome data of the prepared complexes indicates tha ...201121782500
isolation and characterization of stemphylium sedicola sbu-16 as a new endophytic taxol-producing fungus from taxus baccata grown in iran.in this study, a total of 25 endophytic fungi were successfully isolated from the inner bark of taxus baccata grown in iran by the aseptic technique. genomic dna was extracted from isolated endophytic fungi and subjected to polymerase chain reaction (pcr) analysis for the presence of the taxus taxadiene synthase (ts) gene, which encodes the enzyme catalyzing the first committed step of taxol biosynthesis. four of 25 isolated endophytic fungi isolates showed pcr positive for the ts gene. subseque ...201122211912
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