Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effects of osmotic stresses on the proteins concentration and pattern of eriocheir sinensis blood. | 1977 | 11919 | |
| [concentration of 3' : 5'-amp in various tissues of the chinese crab eriocheir sinensis milne edwards]. | 1976 | 65964 | |
| osmoregulation of the chinese crab eriocheir sinensis as related to the activity of the (na+, k+) atpase [proceedings]. | 1977 | 71123 | |
| influence of salinity on the lipid composition and the fatty-acid pattern of muscle and hepatopancreas of the chinese crab eriocheir sinensis. | the values of principal lipids and fatty acids from muscle and hepatopancreas of crab e. sinensis living in fresh water or in sea water are determined. lipid content from these two tissues does not significantly change following a variation in the salinity of the external medium. nevertheless a slight decrease in the triglyceride concentration (+/- 20%) of hepatopancreas and a very slight increase in the polyunsaturated fatty-acid amount of phospholipids isolated from muscle and hepatopancreas a ... | 1978 | 80994 |
| [cytochemical observations of hemocytes in decapod brachyuran crustacea (author's transl)]. | hemocytes from two species of crabs, eriocheir sinensis and carcinus maenas, have been submitted to different cytochemical reactions in order to bring out the nature of granules' content, either by specific coloration or be enzymatic digestion. light as well as electron microscopy has been used. the granules appeared to be made essentially of basic proteins, rich in arginin and disulfide bridges but poor in tyrosin. these proteins are linked with nonglycogenic carbohydrates. glycogen deposits of ... | 2002 | 127769 |
| [cytochemical and ultrastructural study of oocyte development of the crab eriocheir sinensis. ii. oogenesis after removal of the eyestalk]. | the effect produced by an eyestalk removal have been studied on eriocheir females at different physiological stages. in juvenile and prepuberal crabs, the operation induces an important rise of the oocyte diameter. only a few variations are observed in puberal females oocytes. cytological changes are found at first at the nucleolar level. the granular area increases and the nucleolar vacuoles volume decreases. then the granules (precursor material to endogenous yolk) disappear in the reticulum c ... | 1976 | 1020948 |
| [cytochemical and ultrastructural study of oocyte development in the crab eriocheir sinensis. i. natural ovogenesis]. | 1976 | 1035078 | |
| invertebrate epithelial na+ channels: amiloride-induced current-noise in crab gill. | epithelial sheets (including cuticle) from posterior gills of the freshwater-adapted euryhaline crab eriocheir sinensis were obtained according to the method of schwarz and graszynski ((1989) comp. biochem. physiol. 92a, 601-604; (1989) verh. dtsch. zool. ges. 82, 211 and (1989) arch. int. physiol. biochim. 97, c45). with external nacl-saline, the outward-directed short-circuit current (isc) could hardly be influenced by external amiloride up to 100 mumol/l but was, on the contrary, strictly dep ... | 1992 | 1316781 |
| acid-base changes on transfer between sea- and freshwater in the chinese crab, eriocheir sinensis. | the time course of changes in hemolymph ph, pco2 and bicarbonate concentration were followed in euryhaline chinese crabs, eriocheir sinensis, after transfer from seawater (sw) to freshwater (fw) and vice versa. in order to correlate these changes with the animal's ionic status, hemolymph osmolarity and na+ and cl- concentrations as well as net exchanges of acid-base equivalents with the external water were also measured. transfer from sw to fw induced a metabolic alkalosis which peaked after 3 d ... | 1992 | 1604063 |
| when are resting water-breathers lacking o2? arterial po2 at the anaerobic threshold in crab. | the minimum arterial o2 partial pressure (pao2) at which, in resting conditions, o2 consumption (mo2) can be maintained and below which anaerobic metabolism is initiated was studied in the crabs eriocheir sinensis and carcinus maenas at 15 degrees c. arterial po2, mo2 (in e. sinensis), blood lactate concentration ([lact]b) and blood copper concentration ([cu]b, an index of the blood o2 carrying capacity) were determined after 24 h exposure to inspired po2 (pio2) ranging from 2.7-2.1 kpa. they we ... | 2013 | 1626143 |
| [properties of lactate dehydrogenase from the muscle of "eriocheir sinensis" m. edw]. | 1970 | 4096963 | |
| [effect of different salts on beta-hydroxyacyl-coa dehydrogenase and beta-acetoacetyl-coa thiolase activities in muscles of eriocheir sinensis and homarus vulgaris]. | 1972 | 4114869 | |
| [on the mechanism of regulation of the intracellular free amino acid concentration, in eriocheir sinensis, during osmotic adaptation]. | 1964 | 4157545 | |
| gill-tissue respiration in the crab eriocheir sinensis. | 2015 | 4451906 | |
| glucose metabolism during osmotic stress in isolated axons of eriocheir sinensis. | 1974 | 4620978 | |
| isosmotic regulation in isolated surviving nerves of eriocheir sinensis milne edwards. | 1969 | 5366189 | |
| changes in the amino-acid concentration in blood and muscle of eriocheir sinensis during hypoosomtic stress. | 1970 | 5452522 | |
| phospholipid composition and metabolism of crustacean gills as related to changes in environmental salinities: relationship between na+-k+-atpase activity and phospholipids. | the phospholipid composition and metabolism are studied in crustacean gills. it is reported that branchiae are rich in pc, pe and dpg and abundant in long chain polyunsaturated fatty acids (20:4 omega 6 and 20:5 omega 3 acids). the pathways of phospholipids synthesis appear similar to those described for vertebrates. it is demonstrated that there exist significant differences in the level of phosphatides between the anterior and posterior gills of eriocheir sinensis. no matter what the salinity, ... | 1984 | 6088168 |
| a morphological study on gills of a crab acclimated to fresh water. | the gills of the fully euryhaline chinese crab eriocheir sinensis were studied by light and electron microscopy. in these phyllobranchiates, the gills consist of a double row of lamellae extending laterally from a central shaft. haemolymph flow pattern inside the gill is described and the existence of a complex secondary vascularization inside the platelets is reported. it is shown that important differences exist between the ultrastructure of the three anterior and the three posterior pairs of ... | 1983 | 6636122 |
| [effect of organ ablation on oogenesis in the crab eriocheir sinensis in the natural state and after removal of y organ]. | 1981 | 7227798 | |
| distribution of presumptive chemosensory afferents with fmrfamide- or substance p-like immunoreactivity in decapod crustaceans. | in five species of decapod crustaceans--cherax destructor (crayfish), carcinus maenas (crab), homarus americanus (clawed lobster), eriocheir sinensis (crab), macrobrachium rosenbergii (shrimp)--immunocytochemical stainings revealed the presence of sensory afferents with fmrfamide-like immunoreactivity in the central nervous system. these afferents were extremely thin, very numerous, and innervated all sensory neuropils except the optic and olfactory lobes. in their target neuropils they gave ris ... | 1997 | 9037486 |
| distribution of centrifugal neurons targeting the soma clusters of the olfactory midbrain among decapod crustaceans. | to determine the distribution of two systems of centrifugal neurons innervating the soma clusters of the olfactory midbrain across decapod crustaceans, brains of the following nine species comprising most infraorders were immunostained with antibodies against dopamine and the neuropeptides substance p and fmrfamide: macrobrachium rosenbergii, homarus americanus, cherax destructor, orconectes limosus, procambarus clarkii, astacus leptodactylus, carcinus maenas, eriocheir sinensis and pagurus bern ... | 1997 | 9106436 |
| cyclic amp stimulation of electrogenic uptake of na+ and cl- across the gill epithelium of the chinese crab eriocheir sinensis | split gill lamellae (epithelium plus cuticle) of hyperregulating chinese crabs acclimated to fresh water were mounted in a modified ussing chamber. active and electrogenic absorption of sodium and chloride were measured as positive amiloride-sensitive and negative cl--dependent short-circuit currents (ina, icl), respectively. both currents were characterized before and after treatment of the tissue with theophylline or dibutyryl cyclic amp. both drugs increased ina and icl. a simple circuit anal ... | 1994 | 9317551 |
| a v-atpase drives active, electrogenic and na+-independent cl- absorption across the gills of eriocheir sinensis | using biochemical and electrophysiological techniques, we have examined the proposal that an h+-atpase is involved in cl- uptake across the gills of the chinese crab eriocheir sinensis. bafilomycin a1 (1 µmol l-1), a specific inhibitor of v-atpases, was used to investigate the importance of this h+-translocating enzyme in cl- transport across the gill. in homogenates of ion-transporting posterior gills, we found the activity of a bafilomycin-sensitive v-atpase to be markedly higher than in ... | 1995 | 9318532 |
| active and electrogenic absorption of na + and cl- across posterior gills of eriocheir sinensis: influence of short-term osmotic variations | split lamellae of posterior gills of chinese crabs (eriocheir sinensis) acclimated to fresh water were mounted in a modified ussing-type chamber, and the transepithelial short-circuit current and conductance were measured. the epithelium shows independent active and electrogenic absorption mechanisms for na+ and cl- that can be measured as positive and negative short-circuit currents, respectively, in the absence of the counter ion. increasing the osmolarity of the haemolymph-side saline by addi ... | 1996 | 9318689 |
| active nacl absorption across split lamellae of posterior gills of the chinese crab eriocheir sinensis: stimulation by eyestalk extract. | split lamellae of the posterior gills of freshwater-adapted chinese crabs (eriocheir sinensis) were mounted in a modified ussing-type chamber, and active and electrogenic absorption of na(+) and cl(-) were measured as positive (i(na)) or negative (i(cl)) short-circuit currents. haemolymph-side addition of eyestalk extract stimulated i(cl) by increasing both the transcellular cl(-) conductance and the electromotive force for cl(-) absorption. the effect was dose-dependent. boiling the eyestalk ex ... | 2000 | 10729285 |
| is the digestive tract an important access route for mercury in the chinese crab eriocheir sinensis (crustacea, decapoda)? | 2000 | 10757667 | |
| high pressure and glycolytic flux in the freshwater chinese crab, eriocheir sinensis. | the hexose part of glycolysis has been studied in the freshwater chinese crab eriocheir sinensis exposed to high pressure (101 ata, i.e. 1000 m depth) at 14 degrees c and in normoxic conditions. glycolytic fluxes (from glucose, ja and from glucose 6 phosphate, jb) have been determined using nadh depletion during the conversion of dihydroxy acetone phosphate into alpha-glycerol phosphate. measurements have been performed at 14 and 19 degrees c. pressure exposure induces an increase of glycolytic ... | 2000 | 11026665 |
| molecular characterization of v-type h(+)-atpase (b-subunit) in gills of euryhaline crabs and its physiological role in osmoregulatory ion uptake. | the vacuolar-type h(+)-atpase (v-atpase) has been implicated in osmoregulatory ion uptake across external epithelia of a growing variety of species adapted to life in fresh water. in the present study, we investigated whether the v-atpase may also function in a euryhaline species that tolerates brackish water (8 salinity) but not fresh water, the shore crab carcinus maenas. cdna coding for the regulatory b-subunit of the v-atpase was amplified and sequenced from c. maenas gills and partially seq ... | 2001 | 11104708 |
| effects of water salinity on acid-base balance in decapod crustaceans. | extracellular acid-base balance in decapod crustaceans is influenced by water salinity, although the nature of this relationship is unclear. in euryhaline crabs, a decrease in salinity results in a metabolic alkalosis in the haemolymph and an increase in salinity results in a metabolic acidosis. alterations in acid-base status by external changes in salinity are thought to be secondary to the adjustments required for ionic and osmotic regulation. in the present study, acid-base adjustments in th ... | 2001 | 11171423 |
| ionic balance in the freshwater-adapted chinese crab, eriocheir sinensis. | ionic regulation by the gills of the freshwater-adapted chinese crab, eriocheir sinensis, was examined. the balance of uptake and loss of nacl in crabs living in freshwater was established. urine production was measured directly by cannulating the nephropores. daily urinary loss of na+ is equivalent to 16% of the haemolymph na+ content and is substantially higher than that based on data from indirect measurements reported in the literature. weight and area of anterior and posterior gills are pro ... | 2001 | 11409624 |
| variation in lipid composition of chinese mitten-handed crab, eriocheir sinensis during ovarian maturation. | this experiment was conducted to investigate the variation in lipid composition during the ovarian maturation of the crab eriocheir sinensis. the chinese mitten-handed crab broodstock was divided into six different maturation periods according to the size and color of ovary. ovary, hepatopancreas, muscle, and hemolymph of broodstock in different maturation periods were analyzed for total lipid and fatty acids using gas chromatography, and lipid classes by thin-layer chromatography. the ovarian l ... | 2001 | 11470448 |
| reproduction response of chinese mitten-handed crab (eriocheir sinensis) fed different sources of dietary lipid. | the effect of feeding three semi-purified diets containing different lipid sources (anchovy oil, soybean oil and pork lard) on fecundity, hatchability and egg fatty acid composition of chinese mitten-handed crab (eriocheir sinensis) broodstock was compared with a fresh clam diet in a 6-month feeding trial. broodstock crabs fed the diet containing pork lard showed poor fecundity and low hatchability. crabs fed the diet containing soybean oil showed improved fecundity; however, no significant impr ... | 2002 | 11867293 |
| the environmental consequences of alien species in the swedish lakes mälaren, hjälmaren, vänern and vättern. | twenty alien species have become established in the lakes mälaren, vänern, vättern and hjälmaren. intentional introductions include fish and the signal crayfish from north america, ornamental plants, and the canada goose. unintentional introductions include the crayfish plague introduced with infected crayfish, the zebra mussel, and chinese mitten crab introduced with ballast water. the introduction of pathogens and parasites, in particular the crayfish plague, to the lakes has had the greatest ... | 2001 | 11878025 |
| dopamine d1 receptors in the gills of chinese crab eriocheir sinensis. | our investigations indicate the existence of binding sites for [3h]sch23390 on crude membrane preparations in the anterior and posterior gills of the chinese crab, eriocheir sinensis acclimated to freshwater (fw) or seawater (sw). maximum specific binding in the posterior gills is always higher than in the anterior gills, independent of saline acclimation. kd values are similar in the two regions, suggesting the same affinity of both types of gills for the ligand, either from fw or from sw crabs ... | 2002 | 11976058 |
| rickettsia-like organism associated with tremor disease and mortality of the chinese mitten crab eriocheir sinensis. | we report the discovery of a rickettsia-like organism (rlo) in cultured freshwater chinese mitten crab eriocheir sinensis. the rlo caused tremor disease and was apparently responsible for a mass mortality (30 to 90%) in 2 provinces in southeast china. moribund crabs were investigated from different districts during outbreaks in 1999 and 2000. with electron microscopy, 3 different pathogens were detected in moribund crabs: a rickettsia-like organism (rlo), virus-like particles (vlp) and a microsp ... | 2002 | 12005237 |
| trace metal uptake by the chinese mitten crab eriocheir sinensis: the role of osmoregulation. | changes in salinity affect the bioavailability and consequent uptake of trace metals by euryhaline invertebrates. in many cases, salinity-related effects on metal uptake can be explained by changes in chemical speciation but salinity may also influence uptake indirectly through its action on osmoregulatory mechanisms. specifically, it can be hypothesised that trace metal uptake may be reduced at salinities approaching the isosmotic point of a species because, at this point, there is reduced acti ... | 2002 | 12054105 |
| effects of atrazine on osmoregulation in the chinese mitten crab, eriocheir sinensis. | this study integrates results from acute contamination with atrazine of isolated perfused gills, and from in vivo chronic contamination of euryhaline chinese mitten crabs, eriocheir sinensis, acclimated to freshwater. atrazine 1 mg/l in contact with the basolateral membrane (in) increases the transepithelial potential difference (tep) from -20.8 +/- 4.9 to -29.7 +/- 3.8 mv in isolated perfused posterior gills (p < 0.01). this effect is only partially explained by a modification of na(+) and cl(- ... | 2002 | 12161172 |
| study on the transmission of tremor disease (td) in the chinese mitten crab, eriocheir sinensis (crustacea: decapoda). | 2002 | 12507490 | |
| [starvation metabolism in parent chinese mitten-handed crab (eriocheir sinensis)]. | the experiment was conducted to study the influence of starvation on the metabolism of parent chinese mitten-handed crab (eriocheir sinensis) with weights of 54.59 (+/- 2.37) g under 20 (+/- 0.5) degree c during oct. to dec., 1998. the results showed oxygen consumption, co2 production and ammonia-n excretion of the parent crab being starved for 30 days were 50.0%, 63.4%, and 59.1% of those of the fed crab, respectively. the course of oxygen consumption reduction could be divided into four phases ... | 2002 | 12625003 |
| [on the chitin digestive bacteria from larval gut of eriocheir sinensis]. | the foregut, mid-gut and hind gut of eriocheir sinensis from the first zoea to megalopa were dissected under asepsis condition. bacteria were separated by plate culture after liquid medium culture. a chitin digestive bacterium was separated from the first zoea foregut. the chitin digestive bacteria weren't founded in the same experiment from the second zoea to megalopa. the chitin digestive bacteria showed roundness, protuberance, glassy humid, margin regular, milk-yellow, aerotolerant anaerobe, ... | 2003 | 12924151 |
| [inhibition by basic dyes of the active transport of mineral material by isolated gills of the crab eriocheir sinensis m. edw..]. | 1953 | 13139730 | |
| [effect of temperature on the steady state of nonprotein amino acid pool of muscles of the crab eriocheir sinensis]. | 1955 | 13239282 | |
| [concentration of the external medium and the steady state of nonprotein amino acid pool in muscles of the crab eriocheir sinensis]. | 1955 | 13239289 | |
| [antibiotic inhibition of the active transport of sodium ions across the isolated gills of the crab eriocheir sinensis]. | 1957 | 13425725 | |
| [presence of an acetal phosphatide in the gills of the crab eriocheir sinensis (m. edw.), in relation to the mechanism of the active transport of mineral ions]. | 1958 | 13560060 | |
| molecular systematics of the asian mitten crabs, genus eriocheir (crustacea: brachyura). | to help resolve phylogenetic relationships among the mitten crabs, complete sequences of the nuclear dna internal transcribed spacer (its) and portions of the mitochondrial genome corresponding to the cytochrome oxidase i (coi), were sequenced for all asian mitten crabs of the genus eriocheir and seven species of the grapsoidea. the resulting phylogeny supports the establishment of a separate genus neoeriocheir, but does not provide justification for the recognition of platyeriocheir. a female m ... | 2003 | 13678686 |
| [active osmotic constituents of muscle of the chinese crab, eriocheir sinensis, adapted to fresh water or to sea water]. | 1962 | 13873024 | |
| [adaptation of the chinese crab (eriocheir sinensis) to sea water, showing in fresh water raised values in the amino acid component of muscle]. | 1962 | 13888134 | |
| [intracellular isosmotic regulation in eriocheir sinensis following ablation of the eyestalks]. | 1962 | 13888135 | |
| [variations of magnesium in the blood serum (in relation to the anadromic and catadromic character of migration) in the decapod crustacean eriocheir sinensis]. | 1960 | 14415233 | |
| study on experimental infections of spiroplasma from the chinese mitten crab in crayfish, mice and embryonated chickens. | tremor disease (td) of the chinese mitten crab eriocheir sinensi has become a serious disease in the chinese freshwater culture industry in recent years. the agent, belonging to spiroplasma, was purified from yolk and allantoic fluids of chicken embryos and was then inoculated into the body of crayfish, the abdominal cavity of icr mice and the allantoid of chicken embryos, respectively. thirty-eight days after the inoculation, the mice and crayfish were dissected and their tissues sampled and ob ... | 2003 | 14643405 |
| net uptake of chloride across the posterior gills of the chinese crab (eriocheir sinensis). | two methods are commonly used for the determination of transbranchial net fluxes of na+ and cl-: direct analysis of changes in ion concentrations in the external medium using flame spectrophotometry or titration (net flux method), and measurement of unidirectional ion fluxes by means of radioactive tracers (tracer method). when we applied both methods in the same preparation, the isolated perfused posterior gill of freshwater-acclimated eriocheir sinensis, to determine net fluxes of cl-, the res ... | 2004 | 14720590 |
| uptake of cadmium through isolated perfused gills of the chinese mitten crab, eriocheir sinensis. | using the perfusion method, we compared cadmium accumulation and influx across the gills of the euryhaline chinese mitten crab eriocheir sinensis, exposed to 4.8 microm cadmium in the incubation medium (out). cadmium influx was not observed across posterior gills while it ranged from 0.15 to 6.82 nmol cd g(-1) gill w.w. h(-1) across anterior ones. for these respiratory gills, a strong increase (40 times) was observed when calcium was removed in both incubation and perfusion media while the lack ... | 2004 | 14720604 |
| the first internal transcribed spacer (its-1) of ribosomal dna as a molecular marker for phylogenetic and population analyses in crustacea. | the objective of the present study is to explore the feasibility of using the first internal transcribed spacer (its-1) of ribosomal dna as a molecular marker for studying the interspecific and intraspecific genetic variations among crustaceans. we designed primers that could amplify its-1 from a majority of taxonomic groups of crustaceans. the gene was found to exhibit a high degree of length polymorphism among different groups, ranging from 182 bp in the barnacle balanus amphitrite to approxim ... | 2001 | 14961351 |
| mass mortality of larval eriocheir sinensis (decapoda: grapsidae) population bred under facility conditions: possible role of zoothamnium sp. (peritrichida: vorticellidae) epiphyte. | 2004 | 15145254 | |
| purification of vitellin from the ovary of chinese mitten-handed crab (eriocheir sinensis) and development of an antivitellin elisa. | vitellin was purified from ovaries of mature female chinese mitten-handed crab (eriocheir sinensis) using gel filtration chromatography. analysis by native page showed the vitellin had a native molecular mass of 520 kda, while denaturing sds-page revealed two subunits of 97 and 74 kda. purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (elisa) was developed. the elisa was sensitive and could effectively detect vitellin in the range of 7.8-500 ... | 2004 | 15253879 |
| a spiroplasma associated with tremor disease in the chinese mitten crab (eriocheir sinensis). | an epidemic of tremor disease has been a serious problem in chinese mitten crabs, eriocheir sinensis, in china in recent years. the disease-causing agent was previously considered to be a rickettsia-like organism. here, analysis of the 16s rrna gene sequence, light and electron microscopy and cultivation in vitro were used to identify the agent. sequence analysis of the 16s rrna gene found it to have 98 % identity with that of spiroplasma mirum. the agent was able to be passed through membrane f ... | 2004 | 15347761 |
| morphology of spiroplasmas in the chinese mitten crab eriocheir sinensis associated with tremor disease. | the structure of spiroplasmas in the chinese mitten crab associated with tremor disease was studied by transmission electron microscopy techniques, including both negative staining and ultrasectioning. a spiral structure, which is a typical form of spiroplasmas, could be detected and the observations showed for the first time the presence of spiroplasmas in the cells of the crab. propagation of spiroplasmas within the cells presented various forms that could be sorted into three morphological ty ... | 2004 | 15380550 |
| mercury burdens in chinese mitten crabs (eriocheir sinensis) in three tributaries of southern san francisco bay, california, usa. | chinese mitten crabs (eriocheir sinensis), endemic to asia, were first reported in the san francisco bay in 1992. they are now established in nearly all san francisco bay tributaries. these crabs accumulate more metals, such as mercury, than crustaceans living in the water column. because their predators include fish, birds, mammals and humans, their mercury burdens have an exceptional potential to impact the ecosystem and public health. we sought to elucidate the potential threat of mitten crab ... | 2005 | 15519723 |
| purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis. | a new reovirus was recently isolated from a freshwater crab, the chinese mitten crab, eriocheir sinensis, in china. the complete viral particles are 55 nm in diameter, icosahedral, non-enveloped and have a mean buoyant density of 1.39 g cm(-3) in cscl gradient. the viral genome is composed of 12 pieces of dsrna with an electrophoretic pattern of 3/4/2/3. this virus infects connective tissue of the gills, gut and hepatopancreas. partial cdna cloning and sequence analysis showed that the rna-depen ... | 2004 | 15575876 |
| cadmium, zinc and the uptake of calcium by two crabs, carcinus maenas and eriocheir sinensis. | the uptake of dissolved cadmium and zinc by crustaceans can usually be explained by the passive process of facilitated diffusion involving a transport protein in the membranes of permeable surfaces. cadmium ions will also enter via uptake routes for calcium, given the similar size of the two free ions. this study has investigated the interaction of cadmium (and comparatively zinc) and calcium uptake in two crabs that show different permeability responses to changes in salinity, with consequently ... | 2005 | 15748747 |
| mitochondrial genome of the chinese mitten crab eriocheir japonica sinenesis (brachyura: thoracotremata: grapsoidea) reveals a novel gene order and two target regions of gene rearrangements. | we determined the complete 16,354 bp mitochondrial dna sequence of the chinese mitten crab eriocheir japonica sinesnesis. it consists of 13 protein coding genes (pcgs), 2 rrnas, and 22 trnas, typical of metazoan mitochondrial genomes. with respect to the ancestral crustacean mt gene order, a pcg, the rrnas, and 12 of the trnas appear to be rearranged. this rearrangement is novel in the arthropods and suggests an accelerated rate of mt genome rearrangement in this brachyuran lineage based on the ... | 2005 | 15780981 |
| hyper-osmoregulatory capacity of the chinese mitten crab (eriocheir sinensis) exposed to cadmium; acclimation during chronic exposure. | the aim of this study was to evaluate the effects of waterborne cadmium on hyper-osmoregulatory capacity of the chinese mitten crab eriocheir sinensis acclimated to freshwater. for this purpose, crabs were submitted to acute (0.5 mg cd l(-1) for 1, 2 or 3 days), chronic (10 or 50 microg cd l(-1) for 30 days) or chronic, immediately followed by acute, exposure. while no effect was observed after 1 or 2 days, hemolymph osmolality, na(+) and cl(-) concentrations were significantly reduced after 3 d ... | 2005 | 15792620 |
| tissue-specific cadmium accumulation and metallothionein-like protein levels during acclimation process in the chinese crab eriocheir sinensis. | aquatic organisms chronically exposed to cadmium can increase their resistance to a subsequent elevated exposure. in order to investigate mechanisms involved in acclimation process in the chinese crab eriocheir sinensis, we compared cd level as well as metallothionein-like protein (mtlp) content in different tissues after direct acute exposure (i.e. 500 microg cd l(-1) for 3 days), and after acute following chronic (i.e. 10 or 50 microg cd l(-1) for 30 days) exposure. cadmium accumulation occurr ... | 2005 | 15792621 |
| a novel spiroplasma pathogen causing systemic infection in the crayfish procambarus clarkii (crustacea: decapod), in china. | a novel disease of crayfish procambarus clarkii appeared in the summer of 2004 in freshwater aquaculture in jiangsu province of china. light and transmission electron microscopy (tem), molecular biological methods and in vitro culture were used to identify the pathogen. the agent was unique in having a helical morphology and rotary motility as observed by phase-contrast light microscopy and was found in haemolymph, muscles, nerves and connective tissues by smear method and tem. ultra-thin sectio ... | 2005 | 16000238 |
| differential protein expression profiles in anterior gills of eriocheir sinensis during acclimation to cadmium. | using a proteomic approach, we characterized different protein expression profiles in anterior gills of the chinese mitten crab, eriocheir sinensis, after cadmium (cd) exposure. two experimental conditions were tested: (i) an acute exposure (i.e. 500 microg cd l(-1) for 3 days) for which physiological, biochemical and ultrastructural damage have been observed previously; (ii) a chronic exposure (i.e. 50 microg cd l(-1) for 30 days) resulting in physiological acclimation, i.e. increased resistanc ... | 2006 | 16249038 |
| the site of vitellogenin synthesis in chinese mitten-handed crab eriocheir sinensis. | the objective of this study was to investigate the synthesis site of vitellogenin in the chinese mitten-handed crab, eriocheir sinensis. using the rt-pcr techniques, the cdna fragments isolated from the ovaries of vitellogenic female crab, we found that its deduced amino acid sequence had a high identity with that from other decapods crustacean vitellogenin. this cdna fragments were used as probes to examine the transcription of mrnas encoding the vg. the mrna expression was observed in vitellog ... | 2006 | 16480909 |
| pharmacokinetics and tissue distribution of enrofloxacin and its metabolite ciprofloxacin in the chinese mitten-handed crab, eriocheir sinensis. | the pharmacokinetics of enrofloxacin and its active metabolite ciprofloxacin were investigated in the chinese mitten-handed crab after a single intramuscular injection of enrofloxacin at 5.0mg/kg body weight. the tissue concentrations of enrofloxacin and ciprofloxacin were determined simultaneously by a high-performance liquid chromatography (hplc) method. the data were analyzed with practical pharmacokinetic program 3p97. the highest average concentrations of enrofloxacin in liver, muscle, gill ... | 2006 | 16962551 |
| ultrastructural study on a novel microsporidian, endoreticulatus eriocheir sp. nov. (microsporidia, encephalitozoonidae), parasite of chinese mitten crab, eriocheir sinensis (crustacea, decapoda). | a microsporidian pathogen, infecting the epithelial cells of the hepatopancreas of chinese mitten crab, eriocheir sinensis, was studied by electron microscopy. the detailed ultrastructure of life cycle of the pathogen including proliferative and sporogonic developmental stages are provided. all stages of the parasite are haplokaryotic and develop in a vacuole bounded by a single membrane in contact with host cell cytoplasm. sporogenesis is synchronous with the same developmental stage in one vac ... | 2007 | 17097104 |
| occurrence and density of vibrio parahaemolyticus in live edible crustaceans from markets in china. | vibrio parahaemolyticus is a marine bacterium causing foodborne disease. occurrence of the bacterium was investigated in six species of edible crustaceans available from markets in mainland china. the bacterium was detected in 22 of 45 whole-body, shell, and feces samples, including mitten crabs, which are supposed to be produced in freshwater ponds. the mean densities ranged from 2.8 log cfu/g in mitten crabs (eriocheir sinensis) to 5.1 cfu/g in giant tiger prawns (penaeus monodon). in hemolymp ... | 2006 | 17133820 |
| effect of waterborne copper on the microstructures of gill and hepatopancreas in eriocheir sinensis and its induction of metallothionein synthesis. | effects of different waterborne copper concentrations (0.00, 0.01, 0.10, 1.00, and 5.00 mg/l) on microstructures of gill and hepatopancreas, and in inducing metallothionein (mt) synthesis were studied in chinese mitten crab eriocheir sinennsis. the results indicated that copper accumulation caused histologic damage to gill and hepatopancreas and induced mt synthesis in hepatopancreas. no significant differences were found in microstructures between 0.01 mg/l copper treatment and the control grou ... | 2007 | 17165106 |
| genetic population structure and contemporary dispersal patterns of a recent european invader, the chinese mitten crab, eriocheir sinensis. | genetic studies of recently established populations are challenging because the assumption of equilibrium underlying many analyses is likely to be violated. using microsatellites, we investigated determinants of genetic structure and migration among invasive european-chinese mitten crab populations, applying a combination of traditional population genetic analyses and nonequilibrium bayesian methods. consistent with their recent history, invasive populations showed much lower levels of genetic d ... | 2007 | 17217341 |
| acute toxicity of the pesticides chlorpyrifos and atrazine to the chinese mitten-handed crab, eriocheir sinensis. | 2006 | 17219314 | |
| metabolic and immune responses in chinese mitten-handed crab (eriocheir sinensis) juveniles exposed to elevated ambient ammonia. | the toxicity of ammonia to eriocheir sinensis juveniles was determined. the 24 h-, 48 h-, 72 h-, 96 h-lc(50) values of total ammonia (tan) were 251.68, 217.61, 156.05, and 119.67 mg l(-1), respectively. following these results, crabs were then exposed for a 2-day period to 20, 40, 60 and 80 mg l(-1) tan and sampled at 3, 6, 24 and 48 h for changes in metabolic parameters (including haemolymph ammonia concentration, glucose, lactate, urea, triacylglycerol, glutamine, and glutamate levels) and imm ... | 2007 | 17317330 |
| effects of long-term exposure to different salinities on the location and activity of na+-k+-atpase in the gills of juvenile mitten crab, eriocheir sinensis. | the euryhalinity of mitten crab, eriocheir sinensis, is based on osmoregulation, and thus on the activity of na(+)-k(+)-atpase. we studied location and activity of this enzyme in gills of juvenile crabs exposed to 5 per thousand, 25 per thousand, and 40 per thousand salinity. the posterior gills showed always a high number of immunopositive cells (ipc), staining with fluorescent antibody against na(+)-k(+)-atpase, covering at 5 per thousand the entire lamellae. at 25 per thousand, they showed fe ... | 2007 | 17321769 |
| a roni-like virus associated with mortalities of the freshwater crab, eriocheir sinensis milne edwards, cultured in china, exhibiting 'sighs disease' and black gill syndrome. | 2007 | 17352794 | |
| [relationships between copepods and eriocheir sinensis larvae in ecological breeding ponds]. | the study on the relationships between two copepod species eurytemor affinis and sinocalanus tenellus and chinese mitten crab eriocheir sinensis larvae in the ecological breeding ponds of e. sinensis larvae in liaohe delta district of panjing city, liaoning province showed that e. affinis and s. tenellus had significant effects on the survival rate of first stage zoae (z1). the higher the density of the two copepod species, the lower the survival rate of z, was (p < 0.01). third stage zoae (z3) ... | 2007 | 17396520 |
| predicting invasion risk using measures of introduction effort and environmental niche models. | the chinese mitten crab (eriocheir sinensis) is native to east asia, is established throughout europe, and is introduced but geographically restricted in north america. we developed and compared two separate environmental niche models using genetic algorithm for rule set prediction (garp) and mitten crab occurrences in asia and europe to predict the species' potential distribution in north america. since mitten crabs must reproduce in water with >15% per hundred salinity, we limited the potentia ... | 2007 | 17494387 |
| [expression and purification of recombinant metallothionein of eriocheir sinensis in e. coli]. | metallothioneins (mt) are potential candidates for medicine development and application. for the purpose of expressing recombinant mt in e. coli, a crab mt cdna cloned into pgem-t was subcloned into pet-gst and then transformed into escherichia coli bl21. the fusion protein was proved to be expressed in both soluble and insoluble form by sds-page and western blot. since metallothionein chelate metal ions, which may effects the physiological process of e. coli, caused the production of recombinan ... | 2007 | 17591271 |
| is hsp70 involved in acclimation to cadmium in the chinese crab, eriocheir sinensis ? | 2007 | 17619794 | |
| effects of water-borne copper on the y-organ and content of 20-hydroxyecdysone in eriocheir sinensis. | the effects of water-borne copper (cu2+) (0.01, 0.10, 1.00, and 5.00 mg/l) on y-organ (yo) microstructure and ultrastructure and on the content of 20-hydroxyecdysone (20-he) in eriocheir sinensis were studied using single gradient factor experiments. results demonstrated that there was no significant difference in yo microstructure and ultrastructure when e. sinensis was exposed to 0.01, 0.10, and 1.00 mg/l water-borne cu2+. however, exposure to 5.00 mg/l cu2+ caused serious damage to the yo mic ... | 2008 | 17674078 |
| phylogenetic analysis of intestinal bacteria in the chinese mitten crab (eriocheir sinensis). | to identify the dominant intestinal bacteria in the chinese mitten crab, and to investigate the differences in the intestinal bacteria between pond-raised and wild crabs. | 2007 | 17714401 |
| predicting the range of chinese mitten crabs in europe. | ecological niche modeling provides a means for predicting the potential future distribution of a nonindigenous species based on environmental characteristics of the species' native range. we applied this method to the chinese mitten crab (eriocheir sinensis), a catadromous crustacean with a long history of invasion in europe. we used genetic algorithm for rule-set prediction to predict the potential european distribution of mitten crab based on its distribution in 42 locations in its native asia ... | 2007 | 17883496 |
| effect of waterborne copper on the microstructures and ultrastructure of the x-organ sinus gland complex in eriocheir sinensis. | the effects of different water-borne copper (cu(2+)) concentrations on the microstructures and ultrastructures of the x-organ sinus gland (xo/sg) in eriocheir sinensis were studied. under a light microscope, no significant differences were found between the treated and control groups. under transmission electron microscopy (tem), the mitochondrial cristae in the neuro-terminal of the xo of the 5.00 mg/l cu(2+) treated group disappeared, or even only a few vacuoles left. no other differences were ... | 2008 | 18058047 |
| a prophenoloxidase from the chinese mitten crab eriocheir sinensis: gene cloning, expression and activity analysis. | prophenoloxidase (propo) is a conserved copper-containing enzyme that plays important roles in immune response of crustaceans and insects. in the present study, the full-length cdna of a prophenoloxidase (designated espropo) was cloned from haemocytes of chinese mitten crab eriocheir sinensis by expressed sequence tag (est) and pcr techniques. the isolated 3549bp full-length cdna of espropo contained a 2040bp open reading frame (orf) encoding a putative propo protein of 679 amino acids, a 5'-unt ... | 2008 | 18160310 |
| molecular cloning, genomic organization and functional analysis of an anti-lipopolysaccharide factor from chinese mitten crab eriocheir sinensis. | anti-lipopolysaccharide factor (alf) represents one kind of basic proteins, which binds and neutralizes lps and exhibits strong antibacterial activity against gram-negative r-type bacteria. the alf gene of chinese mitten crab eriocheir sinensis (milne edwards, 1853) (denoted as esalf) was identified from haemocytes by expressed sequence tag (est) and pcr approaches. the full-length cdna of esalf consisted of 700 nucleotides with a canonical polyadenylation signal-sequence aataaa, a polya tail, a ... | 2008 | 18206230 |
| microsatellite analysis of genetic diversity and population structure of chinese mitten crab (eriocheir sinensis). | chinese mitten crab (eriocheir sinensis) has higher commercial value as food source than any other species of eriocheir in china. to evaluate the germplasm resources and characterize the genetic diversity and population structure of the crabs in different water systems, two stocks and two farming populations were assessed with 25 polymorphic microsallite loci available in public genbank. basic statistics showed that the average observed heterozygosity (ho) amongst populations ranged from 0.5789 ... | 2008 | 18355760 |
| improvement of innate immune responses and defense activity in mitten crab (eriocheir sinensis) by oral administration of beta-glucan. | the beta-glucan (bg), extracted from saccharomyces cerevisiae cell wall, was orally administrated to the mitten crab (eriocheir sinensis) at 0, 1, 5 and 10 g bg/kg diet for 28 days, followed by a challenge with vibrio mimicus by intramuscular injection. growth, phenoloxidase, superoxide dismutase, acid phosphatase and alkaline phosphatase activity were monitored after 14 and 28 days. the results showed an immunomodulatory effect and protection against v. mimicus by dietary supplementation of bg. ... | 2008 | 18568402 |
| phylogenetic analysis of spiroplasmas from three freshwater crustaceans (eriocheir sinensis, procambarus clarkia and penaeus vannamei) in china. | disease epizootics in freshwater culture crustaceans (crab, crayfish and shrimp) gained high attention recently in china, due to intensive developments of freshwater aquacultures. spiroplasma was identified as a lethal pathogen of the above three freshwater crustaceans in previous studies. further characterization of these freshwater crustacean spiroplasma strains were analyzed in the current study. phylogenetic position was investigated by analysis of partial nucleotide sequences of 16s ribosom ... | 2008 | 18621053 |
| effects of osmotic shock on the ultrastructure of cell nuclei in euryhaline and stenohaline crustaceans. | changes in cell ultrastructure in relation to volume regulation following application of osmotic challenges have been but poorly documented up to now. in a previous study, we demonstrated that large and immediate changes in the organization of nuclear chromatin occur in mammalian cells under such conditions. similar chromatin modifications are shown in the present study in the cells of the stenohaline crab maia verrucosa. they are not seen in the cells of the euryhaline species tested, hyperosmo ... | 1991 | 18621193 |
| molecular cloning of cyclin b transcript with an unusually long 3' untranslation region and its expression analysis during oogenesis in the chinese mitten crab, eriocheir sinensis. | the meiotic maturation of oocyte in animals is regulated by maturation promotion factor (mpf), a complex of cdc2 and cyclin b. although the role of mpf during oocyte maturation has been well studied in a wide variety of eukaryotic organisms, little is known for crustacean species. in this study, a full-length cdna of cyclin b was cloned from the chinese mitten crab using degenerate rt-pcr and race methods. the crab cyclin b cdna was 3,794 bp containing an unusually long 3' untranslation region ( ... | 2009 | 18756373 |
| functional annotation and analysis of expressed sequence tags from the hepatopancreas of mitten crab (eriocheir sinensis). | due to its popularity as a traditional food, intensive harvesting of the mitten crab (eriocheir sinensis) is common and has lead to an increase in disease incidence, resulting in catastrophic losses to crab aquaculture. the hepatopancreas of e. sinensis is not only an important digestive organ but also an indispensable immune organ. we constructed a nonnormalized cdna library from the hepatopancreas of e. sinensis and acquired 3,297 high-quality expressed sequence tags representing 1,178 unigene ... | 2009 | 18815839 |
| cdna cloning, characterization and mrna expression of a pacifastin light chain gene from the chinese mitten crab eriocheir sinensis. | the pacifastin family, characterized by several conserved arrays of six cysteine residues, is a newly identified serine protease inhibitor (spi) family discovered uniquely in arthropods and plays important roles in multiple biological processes. in the present study, the full-length cdna of a pacifastin light chain (designated esplc) was cloned from the chinese mitten crab eriocheir sinensis by expressed sequence tags (ests) and pcr techniques. the 1036bp esplc cdna contained an 831bp open readi ... | 2008 | 18817876 |
| acute tolerance and metabolic responses of chinese mitten crab (eriocheir sinensis) juveniles to ambient nitrite. | the lethal concentration of nitrite to the chinese mitten crab eriocheir sinensis was tested by exposing the animals to 17.78, 23.71, 31.62, 42.17, and 56.23 mg nano2 l(-1) at 20 degrees c for 24, 48, 72, and 96 h. the corresponding lc50 value for each time exposure was 43.87 (38.70-51.70), 40.24 (34.88-46.01), 38.87 (33.72-46.01) and 38.87 (33.72-46.01) mg nano2 l(-1) or 29.25 (25.80-34.47), 26.83 (23.25-30.67), 25.91(22.48-30.67), 25.91(22.48-30.67) mg no2-n l(-1), respectively. the physiologi ... | 2009 | 18950735 |
| molecular cloning and characterization of peroxiredoxin 6 from chinese mitten crab eriocheir sinensis. | peroxiredoxin is a superfamily of antioxidative proteins that play important roles in protecting organisms against the toxicity of reactive oxygen species (ros). in this study, the full-length cdna encoding peroxiredoxin 6 (designated esprx6) was cloned from chinese mitten crab eriocheir sinensis by using rapid amplification of cdna ends (race) approaches. the full-length cdna of esprx6 was of 1076 bp, containing a 5' untranslated region (utr) of 69 bp, a 3' utr of 347 bp with a poly (a) tail, a ... | 2009 | 18992822 |
| on the role of cdc2 kinase during meiotic maturation of oocyte in the chinese mitten crab, eriocheir sinensis. | cdc2 kinase is a catalytic subunit of maturation-promoting factor (mpf), a central factor for inducing the meiotic maturation of oocyte. to understand the role of cdc2 kinase on the oocyte maturation in crustacean, a complete cdna sequence of cdc2 kinase was cloned from chinese mitten crab eriocheir sinensis and its spatial-temporal expression profiles were analyzed during oogenesis at rna and protein levels. the crab cdc2 cdna (1364 bp) encodes for a 299 amino acids protein with calculated mole ... | 2009 | 19118638 |
| mitochondrial dna-inferred population structure and demographic history of the mitten crab (eriocheir sensu stricto) found along the coast of mainland china. | the mitten crab, eriocheir sensu stricto, is economically important in east asia, although it is an invasive species in europe and north america. little is known about its population structure and historical demography in its native range, especially along the pacific coast of china. we collected mitochondrial cytochrome oxidase subunit ii and cytochrome b sequences from 154 individuals distributed in the rivers along the chinese coast and 15 individuals from japan. phylogenetic analysis resulte ... | 2008 | 19160479 |
| molecular cloning, mrna expression, and characterization of hsp90 gene from chinese mitten crab eriocheir japonica sinensis. | hsp90 is a highly conserved molecular chaperone important in the maturation of a broad spectrum of proteins. using expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques, an hsp90 gene designated as ejshsp90 was cloned and characterized from the chinese mitten crab eriocheir japonica sinensis. the full-length cdna of ejshsp90 is 2,517 bp and contains an open reading frame of 2157 bp which encodes a 718 amino acid polypeptide (82.8 kda) bearing characteristics of the ... | 2009 | 19166961 |
| prevalence and potential pathogenicity of vibrio parahaemolyticus in chinese mitten crabs (eriocheir sinensis) harvested from the river thames estuary, england. | chinese mitten crabs (eriocheir sinensis) have been described as an alien invasive species in the river thames, united kingdom, and elsewhere in europe. the crabs can cause considerable physical damage to the riverbeds and threaten native ecosystems. trapping has been considered an option, but such attempts to control mitten crab populations in germany in the 1930s failed. in the united kingdom, it has been suggested that commercial exploitation of the species could be employed as a control opti ... | 2009 | 19205465 |