Publications

effect of cell immobilization and organic solvents on sulfoxidation and steroid hydroxylation by mortierella isabellina.	the effects of calcium alginate bead immobilization and the presence of organic solvents on two bioconversion reactions carried out by mortierella isabellina atcc 42613 have been investigated. these reactions, the 14 alpha-hydroxylation of progesterone and the sulfoxidation of thioanisole, both proceed in high yield using resting-cell bioconversions, but are not carried out by alginate bead preparations in the absence of an organic co-solvent, the best results being obtained with 5 or 10% aqueou ...	1992	1368869
biotransformation of dehydroabietic acid with resting cell suspensions and calcium alginate-immobilized cells of mortierella isabellina.	mortierella isabellina atcc 38063 is a zygomycete capable of hydroxylating fish-toxic resin acids which occur in certain pulp mill effluents to nontoxic metabolites. addition of dehydroabietic acid (1) (80 mg/liter) to a freshly inoculated culture of m. isabellina in dextrose-yeast extract broth resulted in precursor disappearance in 28 to 30 h. during growth phase, hydroxylation occurred at c-2, whereas hydroxylation at c-15 and c-16 commenced with onset of stationary phase. alternatively, 1 ad ...	1985	3883900
the production and partial characterization of a monoclonal igg antibody specific for moulds belonging to the order mucorales.	a monoclonal antibody (mab) was raised against extracellular polysaccharides from mucor racemosus after intrasplenic immunization of mice. an indirect elisa and a dot-blot assay were developed with this mab. the igg antibody was found to be very specific for all mould species tested belonging to the order of mucorales, except species belonging to the genus mortierella sensu stricto. no cross-reactions were observed with other moulds or yeasts. the immunoreactivity of the polysaccharides of these ...	1993	7690393
chemical and enzyme-catalysed synthesis of quinoline arene hydrates.	arene hydrates of quinoline have been synthesized by enzyme-catalysed benzylic and allylic hydroxylation of dihydroquinolines in growing cultures of the fungus mortierella isabellina and in liver microsomal preparations. a preference for allylic hydroxylation was generally observed in eukaryotic systems. evidence of epoxidation of dihydroarenes by both the fungal and animal enzyme systems was also obtained. the chemical synthesis of these arene hydrates (5-hydroxy-5,6-dihydroquinoline, 6-hydroxy ...	1994	8000866
fungal biotransformation of organophosphines.	1. the biotransformations of triphenyl phosphite and of triphenyl-, tri-n.-butyl-, diethylphenyl-, and ethylmethylphenyl-phosphines by the fungi mortierella isabellina atcc 42613, helminthosporium species nrrl 4671, and aspergillus foetidus atcc 10254, have been examined. 2. the triaryl and tri-n.-butyl substrates underwent oxidation at phosphorus in low yield, a process attributed largely to auto-oxidation under the experimental conditions used. 3. the alkyl aryl phosphines were enzymically oxi ...	1993	8342299
microbial models of mammalian metabolism. fungal metabolism of phenolic and nonphenolic p-cymene-related drugs and prodrugs. i. metabolites of thymoxamine.	this study was undertaken to validate the use of microbial biotransformation systems for drug metabolism studies. thymoxamine 1 was rapidly hydrolyzed to desacetylthymoxamine (dat) 2 by numerous fungi. other known animal metabolites, such as n-desmethyl-desacetylthymoxamine 3 and desacetylthymoxamine-o-sulfate 6, were produced from dat by mucor rouxii and mortierella isabellina. dat-n-oxide 5, a putative animal microsomal metabolite, was also produced by m. isabellina, in addition, a few strains ...	1997	9172947
microbial models of mammalian metabolism. fungal metabolism of phenolic and nonphenolic p-cymene-related drugs and prodrugs. ii. metabolites of nonphenolic derivatives.	a cymene-derived drug, 3-[4'-(o-ethoxyphenyl)piperazin-1'-yl]-ethyloxy-p-cymene+ ++ (b1178), is not significantly metabolized by fungal microorganisms. on the contrary, one of its metabolites in rat, 3-(piperazin-1'-yl)ethoxy-p-cymene (b1071), is quantitatively converted by cunninghamella echinulata nrrl 3655 into two hydroxylated products: the corresponding phenol derivative and a benzylic alcohol derivative. other strains, such as beauveria bassiana atcc 7159 and mortierella isabellina mmp 108 ...	1997	9172948
microbial biotransformations of a synthetic immunomodulating agent, hr325.	the microbial biotransformation of hr325 [2-cyano-3-cyclopropyl-3-hydroxy-n-(4'-trifluoromethyl-3'-methylphenyl)- propenamide], a synthetic immunomodulating agent, has been investigated in order to be compared with animal metabolism and to prepare some metabolites which are difficult to obtain by chemical methods. several fungal strains are able to completely metabolize this drug. mortierella isabellina nrrl 1757 only achieves a benzylic hydroxylation on the aromatic methyl group, affording in h ...	1997	9377097
biotransformation of arachidonic acid and other eicosanoids by the yeast dipodascopsis uninucleata, the oomycete fungi saprolegnia diclina and leptomitus lacteus and the zygomycete fungus mortierella isabellina.		1997	9561142
enantioselective synthesis and pharmacological evaluation of a new type of verapamil analog with hypotensive and calcium antagonist activities.	the syntheses and evaluation for cardiovascular activity in the rat of both enantiomers of a verapamil analog in which the cyano group has been replaced by hydroxyl.	1999	10100315
production of gamma-linolenic acid by mortierella isabellina grown on hexadecanol.	to optimize the production of linolenic acid by mortierella isabellina grown on hexadecanol.	2001	11696098
production of linolenic acid by mortierella isabellina grown on octadecanol.	the production of linolenic acid in mycelial lipids reached 0.31 mg/ml of culture broth when mortierella isabellina was cultivated in a medium consisting of 2% octadecanol, 1% yeast extract, and 25 mmol/l of mg2+ at 23 degrees c for 5 days. cultivation conditions were studied, and the results showed that (i) a suitable concentration of mg2+ in the medium caused an increase in mycelial mass as well as linolenic acid production; (ii) when incubated at 23 degrees c, maximal linolenic acid productiv ...	2002	11815860
biotransformation of phenylurea herbicides by a soil bacterial strain, arthrobacter sp. n2: structure, ecotoxicity and fate of diuron metabolite with soil fungi.	in order to assess the influence of the aromatic substitution on the ability of a soil bacterial strain, arthrobacter sp. n2, to degrade phenylurea herbicides, biotransformation assays were performed in mineral medium with resting cells of this soil bacterial strain on three phenylurea herbicides (diuron, chlorotoluron and isoproturon). each herbicide considered, led to the formation of only one metabolite detected by hplc analysis. after isolation, the metabolites were identified by nmr and ms, ...	2002	11838430
preparation of enantiopure ketones and alcohols containing a quaternary stereocenter through parallel kinetic resolution of beta-keto nitriles.	racemic 1-methyl-2-oxocycloalkanecarbonitriles have been subjected to bioreduction by the fungus mortierella isabellina nrrl 1757 through a parallel kinetic-resolution process. the u and l alcohols thus obtained (up to >99% ee) were easily separated and oxidized to the r and s ketones, respectively. the process can be then repeated so that both enantiomers of the ketone and two epimers of the alcohol can be obtained in their enantiopure forms.	2002	11871913
comparative molecular field analysis (comfa) for sulfoxidation reactions in mortierella isabellina atcc 42613 and helminthosporium sp. nrrl 4671.	previous models for mechanisms of enzymatic sulfoxidation have been somewhat limited by a lack of knowledge of the essential features of substrate-enzyme versus product-enzyme relationships. computerized methods for modeling ligand-protein (substrate-enzyme) interactions can overcome some of these limitations. specifically, comfa (comparative molecular field analysis) provided a useful general approach in which to evaluate substrate-enzyme and product-enzyme relationships. the present investigat ...	2002	12111398
production of gamma-linolenic acid by disrupted mycelia of mortierella isabellina.	to optimize the production of linolenic acid by disrupted mycelia of mortierella isabellina.	2003	12581380
biotransformations of the cardiovascular drugs mexrenone and canrenone.	microbial transformation studies of the cardiovascular drugs mexrenone (1) and canrenone (2) were conducted. thirty-nine biotransformations of mexrenone and 84 biotransformations of canrenone were analyzed. metabolism of the substrate was observed in the majority of these cases. several monohydroxylated derivatives were detected by hplc-ms-uv and subsequently identified. two new mexrenone derivatives, 11alpha- (3) and 12beta-hydroxymexrenone (4), and the known metabolite 6beta-hydroxymexrenone ( ...	2003	12662091
biotransformation of beta-ketosulfides to produce chiral beta-hydroxysulfoxides.	the biotransformations of a series of substituted phenylthio-2-propanone and benzylthio-2-propanone were carried out using helminthosporium sp. nrrl 4671, mortierella isabellina atcc 42613, or rhodococcus erythropolis igts8. several products gave microbial oxidation of sulfide to sulfoxide and reduction of carbonyl to secondary alcohol, producing beta-hydroxysulfoxides in medium to high enantiomeric and diastereomeric purities. fungal biotransformations using helminthosporium sp. and m. isabelli ...	2003	12759809
delimitation of umbelopsis (mucorales, umbelopsidaceae fam. nov.) based on its sequence and rflp data.	in a continuation of studies started by de ruiter et al. (1993), all known species of the mortierella isabellina-group (micromucor/umbelopsis clade of o'donnell et al. 2001) and a few other mucorales and species of mortierella were investigated by rflp (including its1, 5.8s, its2 and the 5' end of the large subunit rdna gene) and its1 sequence analyses. this monophyletic group is unrelated to mortierella and is only distantly related to the core group of the mucoraceae. m. longicollis falls outs ...	2003	12825503
decolorization of synthetic dyes and production of manganese-dependent peroxidase by new fungal isolates.	two yeasts, debaryomyces polymorphus, candida tropicalis, and two filamentous fungi, umbelopsis isabellina, penicillium geastrivorus, could completely decolorize 100 mg reactive black 5 (rb 5) l-1 within 16-48 h. manganese-dependent peroxidase (mnp) activities between 60 and 424 u l-1 were detected in culture supernatants of three of these organisms indicating the color removal by enzymatic biodegradation but with p. geastrivorus there was no ligninolytic enzyme activity in its culture and the d ...	2003	12882171
single cell oil (sco) production by mortierella isabellina grown on high-sugar content media.	mortierella isabellina cultivated in nitrogen-limited media presented remarkable cell growth (up to 35.9 g/l) and high glucose uptake even with high initial sugar concentrations (e.g. 100 g/l) in media. after nitrogen depletion, significant fat quantities were accumulated inside the fungal mycelia (50-55%, wt/wt oil in dry biomass), resulting in a notable single cell oil production of 18.1 g/l of culture medium. total dry biomass and lipid yields presented greatly increased values (0.34 and 0.17 ...	2004	15288271
repression of reserve lipid turnover in cunninghamella echinulata and mortierella isabellina cultivated in multiple-limited media.	to study patterns of reserve lipid biosynthesis and turnover (degradation) in two oleaginous zygomycetes, namely cunninghamella echinulata and mortierella isabellina under various growth conditions. fatty acid composition of the reserve lipid of both strains was also studied in all growth steps.	2004	15357737
[heteologous expression of mortierella isabellina delta6 -fatty acid desaturase gene in soybean].	gamma-linolenic acid (gla, c18:3delta(6, 9, 12) is one of nutritionally important polyunsaturated fatty acids in human and animal diets. vast majority oilseeds crops do not produce gla. delta6-fatty acid desaturase (d6d) is the rate-limiting enzyme, which catalyzes the conversion of linoleic acid (la) and alpha-linolenic acid (ala) to gla and octadecatetraenoic acid (ota). in this study, a delta6-fatty acid desaturase gene isolated from mortierella isabellina was transformed into some soybean cu ...	2004	15481543
metabolism of daidzein by nocardia species nrrl 5646 and mortierella isabellina atcc 38063.	the phytoestrogen daidzein was metabolized by nocardia species nrrl 5646 to give two metabolites obtained by hydroxylation and methylation. these metabolites were spectrally characterized as 7-methoxy-4'-hydroxyisoflavone (isoformononetin) and 7,8-dimethoxy-4'-hydroxyisoflavone. mortierella isabellina atcc 38063 was able to metabolize daidzein to the unusual metabolite daidzein-4'-rhamnopyranoside.	2005	15896369
[expression of mortierella isabellina delta6-fatty acid desaturase gene in gamma-linolenic acid production in transgenic tobacco].	gamma-linolenic acid (gla, c18:3delta6.9.12) is nutritional and important polyunsaturated fatty acid in human and animal diets. gla play an important role in hormone regulation and fatty acid metabolization. furthermore it is also the biological precursor of a group of molecules, including prostaglandins, leukotrienes and thromboxanes. vast majority of oilseed crops do not produce gla, but linoleic acid (la, c18:2delta9.12) as its substrate. gla is only produced by a small number of oilseed plan ...	2003	15966318
fungal bioconversion of 2,4-dichlorophenoxyacetic acid (2,4-d) and 2,4-dichlorophenol (2,4-dcp).	ninety strains of fungi from the collection of our mycology laboratory were tested in galzy and slonimski (gs) synthetic liquid medium for their ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) and its by-product, 2,4-dichlorophenol (2,4-dcp) at 100 mg l(-1), each. evolution of the amounts of each chemical in the culture media was monitored by hplc. after 5 days of cultivation, the best results were obtained with aspergillus penicilloides and mortierella isabellina for 2,4 ...	2005	16201028
regio- and stereo-selective hydroxylation of abietic acid derivatives by mucor circinelloides and mortierella isabellina.	mucor circinelloides and mortierella isabellina hydroxylated dehydroabietic acid (deha). deha was converted regio- and stereo-selectively by whole cells of mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mm from 14.7 mm deha) after 72 h in the cultivation medium containing 3% (v/v) tween 80. with cells of ma. isabellina, under the same conditions, 20.5 mm (6.5 g l(-1)) 2-hydroxydehydroabietic acid (alpha/beta=81/19) was formed from 26.4 mm deha.	2005	16215830
biotransformation of dehydroabietic, abietic, and isopimaric acids by mortierella isabellina immobilized in polyurethane foam.	polyurethane foam supports immobilization of mortierella isabellina atcc 38063, a zygomycete that hydroxylates and thereby detoxifies resin acids found in certain pulp mill effluents. the fungus becomes entrapped as it threads growing mycelia through the open foam matrix. the tenacity of binding and the amount of biomass bound depend on initial spore numbers and nutrient concentration. optimal dehydroabietic acid transformation occurs with early-stationary-phase foam-bound mycelia suspended in b ...	1988	16347596
cloning and molecular characterization of delta12-fatty acid desaturase gene from mortierella isabellina.	to clone delta(12)-fatty acid desaturase gene of mortierella isabellina and to functionally characterize this gene in vitro and in vivo.	2006	16733854
in vitro susceptibility to fungicides by invertebrate-pathogenic and saprobic fungi.	the effect of five fungicides, benomyl (1 mg/l), dodine (50 mg/l), manzate (100 mg/l), cupric sulphate (200 mg/l) and thiabendazole (4 mg/l) was tested under in vitro conditions on development of 15 isolates of fungi pathogenic for insects and other invertebrates (beauveria brongniartii, culicinomyces clavisporus, duddingtonia flagrans, hirsutella thompsonii, two metarhizium anisopliae, nomuraea rileyi, two isaria/paecilomyces spp., and sporothrix insectorum) and 13 isolates of contaminant fungi ...	2007	17574540
[protoplast transformation of mortierella isabellina with hygromycin b resistance plasmid pd4].	a strain mortierella isabellina m6-22-4, which was sensitive to hygromycin b, was selected by treating parental spores with n-methyl-n' -nitro-n-nitrosoguanidine (mnng). protoplasts of the strain mortierella isabellina m6-22-4 were transformed successfully to hygromycin b resistance using the pd4 plasmid, which contains the escherichia coli hph gene under the control of mortierella alpina his h4.1 promoter. the pd4 plasmid was introduced by peg/cacl2 treatment. transformation frequencies of 1.6 ...	2007	17577994
atrazine degradation by aerobic microorganisms isolated from the rhizosphere of sweet flag (acorus calamus l.).	in presented study the capability of microorganisms isolated from the rhizosphere of sweet flag (acorus calamus) to the atrazine degradation was assessed. following isolation of the microorganisms counts of psychrophilic bacteria, mesophilic bacteria and fungi were determined. isolated microorganisms were screened in terms of their ability to decompose a triazine herbicide, atrazine. our results demonstrate that within the rhizosphere of sweet flag there were 3.8x10(7) cfu of psychrophilic bacte ...	2008	17594524
disruption of the fatty acid delta6-desaturase gene in the oil-producing fungus mortierella isabellina by homologous recombination.	the gamma-linolenic acid-producing fungus mortierella isabellina 6-22 is an important industrial strain. to clarify the biosynthetic pathways for polyunsaturated fatty acids in this strain, a disruption vector pd4mi6, including 5' and 3' regions of the fatty acid delta(6)-desaturase open reading frame as homologous recombination elements and the escherichia coli hygromycin b (hmb) phosphotransferase gene (hph) as selectable marker, was successfully constructed. following transformation of pd4mi6 ...	2007	17619102
novel ent-beyeran-19-oic acids from biotransformations of isosteviol metabolites by mortierella isabellina.	biotransformations of ent-16beta-hydroxybeyeran-19-oic acid ( 1) by mortierella isabellina produced hydroxylated metabolites. the isolated metabolites included three new compounds, ent-14beta,16beta-dihydroxybeyeran-19-oic acid ( 3), ent-12beta-hydroxy-16-oxobeyeran-19-oic acid ( 4), and ent-7alpha,12beta-dihydroxy-16-oxobeyeran-19-oic acid ( 5), and one known compound, ent-7alpha,16beta-dihydroxybeyeran-19-oic acid ( 2). the structural elucidation was achieved by detailed analysis of lc-ms chro ...	2007	18080234
[diversity of facultatively anaerobic microscopic mycelial fungi in soils].	the numbers of microscopic fungi isolated from soil samples after anaerobic incubation varied from tens to several hundreds of cfu per one gram of soil; a total of 30 species was found. this group is composed primarily of mitotic fungi of the ascomycete affinity belonging to the orders hypocreales (fusarium solani, f. oxysporum, fusarium sp., clonostachys grammicospora, c. rosea. acremonium sp., gliocladium penicilloides, trichoderma aureoviride, t. harzianum, t. polysporum, t. viride. t. koning ...	2008	18365728
[breeding of arachidonic acid producing mortierella sabellina by ultraviolet mutation].	we obtained a new mutant with higher growth rate and high capability of producing arachidonic acid after protoplast ultraviolet mutation.	2009	19388263
fatty acid composition in lipid fractions lengthwise the mycelium of mortierella isabellina and lipid production by solid state fermentation.	this paper investigates the correlation between mycelial age and fatty acid biosynthesis. the correlation was investigated by analyzing the lipid composition lengthwise the mycelium of the oleaginous fungus mortierella isabellina, a potential producer of gamma-linolenic acid (gla). young mycelia were rich in polar lipids (glycolipids plus sphingolipids and phospholipids), while neutral lipid content increased in aged mycelia. in young mycelia, each polar lipid fraction contained almost 40% (w/w) ...	2009	19574039
isotope fractionations in the biosynthesis of cell components by different fungi: a basis for environmental carbon flux studies.	abstract the isotope fractionation of carbon from substrates possessing different isotope ratios into fatty acids of polar lipids and amino acids was determined for four different fungi (rhizopus arrhizus, mortierella isabellina, fusarium solani, aspergillus niger). carbon isotope ratios of fungi closely followed that of the substrates. palmitic acid (c16:0), derived from phospholipids, did not display a large carbon isotope fractionation against the substrate. stearic acid (c18:0), however, was ...	2003	19719589
semi-solid state fermentation of sweet sorghum for the biotechnological production of single cell oil.	a semi-solid fermentation process for the production of biodiesel from sweet sorghum is introduced. the microorganism used is the oleaginous fungus mortierella isabellina, which is able to transform efficiently sugar to storage lipid. kinetic experiments were performed at various water content percentages. the fungus consumed simultaneously sugars and nitrogen contained in sorghum and after nitrogen depletion the biomass growth was completed and oil accumulation began. water content of 92% prese ...	2010	19781936
[biomass carbohydrates assimilation and lipid accumulation by mortierella isabellina].	biomass carbohydrates assimilation and lipid accumulation by mortierella isabellina m2 strain were investigated. corn fiber hydrolysate was specially studied. the results showed m. isabellina m2 strain achieved growth and lipid accumulation while glucose, xylose, mannose and arabinose were introduced as single carbon source, respectively. when m. isabellina m2 strain was cultivated on corn fiber hydrolysate with 6% sugars concentration, the biomass reached 18.2 g/l, the lipid content of dry myce ...	2010	20432937
agrobacterium rhizogenes-mediated transformation of a high oil-producing filamentous fungus umbelopsis isabellina.	the agrobacterium rhizogenes-mediated transformation procedure was developed by using the hygromycin b phosphotransferase gene (hph) as a selective marker for the oil-producing fungus umbelopsis isabellina. different conditions were combined to increase the transformation efficiency. the highest efficiency was obtained by using a. rhizogenes strain r105 and a vector with zygomycete promoter. southern blot analysis demonstrated that 71% of transformants contained random integrations of t-dna sequ ...	2010	20453313
[mortierella isabellina lipid-soluble extracts improves lipid-induced renal injury in rats].	to investigate the effect of the mortierella isabellina lipid-soluble extracts on improving hyperlipidemia and renal damage in lipid-induced renal injury rats.	2010	20476584
pioneering fungi from the damma glacier forefield in the swiss alps can promote granite weathering.	fungi were isolated from fine granitic sediments, which were collected at 15 sampling points within a 20 m × 40 m area in front of the damma glacier in the central swiss alps. from the 45 fungal isolates grown on nutrient-rich agar media at 4 °c, 24 isolates were selected for partial sequencing and identification based on the small subunit ribosomal dna. sequencing data revealed that the isolated fungi represented three fungal phyla and 15 species. the weathering potential of 10 of the 15 fungal ...	2011	21356005
modeling of single-cell oil production under nitrogen-limited and substrate inhibition conditions.	sweet sorghum extract was used as substrate for lipid accumulation by the oleaginous fungus mortierella isabellina in batch cultures. various initial sugar (13-91 g/l) and nitrogen (100-785 mg/l) concentrations resulting in various c/n (43-53) ratios were tested. oil accumulation ranged between 43% and 51% corresponding to oil production from 2.2 to 9.3 g/l. a detailed mathematical model was developed. this model is able to adequately predict biomass growth, lipid accumulation, and sugar and nit ...	2011	21449022
modeling lipid accumulation in oleaginous fungi in chemostat cultures: i. development and validation of a chemostat model for umbelopsis isabellina.	lipid-accumulating fungi may be able to produce biodiesel precursors from agricultural wastes. as a first step in understanding and evaluating their potential, a mathematical model was developed to describe growth, lipid accumulation and substrate consumption of the oleaginous fungus umbelopsis isabellina (also known as mortierella isabellina) in submerged chemostat cultures. key points of the model are: (1) if the c-source supply rate is limited, maintenance has a higher priority than growth, w ...	2011	21538015
hydroxylation of dhea, androstenediol and epiandrosterone by mortierella isabellina am212. evidence indicating that both constitutive and inducible hydroxylases catalyze 7α- as well as 7β-hydroxylations of 5-ene substrates.	the course of transformation of dhea, androstenediol and epiandrosterone in mortierella isabellina am212 culture was investigated. the mentioned substrates underwent effective hydroxylation; 5-ene substrates--dhea and androstenediol--were transformed into a mixture of 7α- and 7β- allyl alcohols, while epiandrosterone was converted into 7α- (mainly), 11α- and 9α- monohydroxy derivatives. ketoconazole and cycloheximide inhibition studies suggest the presence of constitutive and substrate-induced h ...	2011	21655574
single cell oil production from rice hulls hydrolysate.	rice hull hydrolysate was used as feedstock for microbial lipids production using the oleaginous fungus mortierella isabellina. kinetic experiments were conducted in c/n ratios 35, 44 and 57 and the oil accumulation into fungal biomass was 36%, 51.2% and 64.3%, respectively. a detailed mathematical model was used in order to describe the lipid accumulation process. this model was able to predict reducing sugar and nitrogen consumption, fat-free biomass synthesis and lipid accumulation. neutral l ...	2011	21875786
modeling of oleaginous fungal biofilm developed on semi-solid media.	an oleaginous fungus, mortierella isabellina, able to transform efficiently sugar to storage lipid, was used as a model microorganism which develops a biofilm structure during the semi-solid fermentation process for the production of biodiesel from sweet sorghum. a mathematical model was developed to describe the fungal oil production in m. isabellina biofilm. the model describes diffusion and consumption of sugars and nitrogen of sweet sorghum and single cell oil production in a biofilm, which ...	2011	21880483
biotransformation of ursolic acid by an endophytic fungus from medicinal plant huperzia serrata.	endophytic fungi were used not only for their producing bioactive products but also for their ability to transform natural compounds. an endophytic fungus, isolated from medicinal plant huperzia serrata, was identified as umbelopsis isabellina based on the internal transcribed spacer of ribosomal dna (rdna-its) region. it was used to transform ursolic acid (1), a pentacyclic triterpene. incubation of ursolic acid with u. isabellina afforded three products, 3β-hydroxy-urs-11-en-28,13-lactone (2), ...	2011	21881267
Modeling growth, lipid accumulation and lipid turnover in submerged batch cultures of Umbelopsis isabellina.	The production of lipids by oleaginous yeast and fungi becomes more important because these lipids can be used for biodiesel production. To understand the process of lipid production better, we developed a model for growth, lipid production and lipid turnover in submerged batch fermentation. This model describes three subsequent phases: exponential growth when both a C-source and an N-source are available, carbohydrate and lipid production when the N-source is exhausted and turnover of accumulat ...	2011	21959583
[screening of high-yield pufas mortierella isabellina strain].	the original strain mortierella isabellina as3.3410 was treated by microwave and ultraviolet. mutated strains were screened by acetyl salicylic acid and low temperature (15°c). a high-yield strain named as a35-4 was successfully selected. the biomass of this strain was 17.9 g/l, oil content was 67.8%, oil production was 12.12 g/l, polyunsaturated fatty acids (pufas) content was 20.2%, and production of pufas was 2.46 g/l, which increased 32.6%, 49.8%, 98.69%, 14.0%, and 125.7% compared with the ...	2011	21993290
solid-state fermentation of mortierella isabellina for lipid production from soybean hull.	soybean hull, generated from soybean processing, is a lignocellulosic material with limited industrial applications and little market value. this research is exploring a new application of soybean hull to be converted to fungal lipids for biodiesel production through solid-state fermentation. mortierella isabellina was selected as the oil producer because of its high lipid content at low c/n ratio. several cultivation factors were investigated, including moisture content, inoculums size, fungal ...	2011	22198865
oxygenated compounds from the bioconversion of isostevic acid and their inhibition of tnf-α and cox-2 expressions in lps-stimulated raw 264.7 cells.	fourteen oxygenated compounds were isolated from the preparative-scale biotransformation of isostevic acid (ent-beyeran-19-oic acid). incubation of it with aspergillus niger bcrc 32720 produced eight metabolites, four with bacillus megaterium atcc 14581, and another four with mortierella isabellina atcc 38063. in addition to their structural elucidation by nmr spectroscopy and hrms, structures of four of these were further confirmed by x-ray diffraction studies. real-time reverse transcription p ...	2012	22226038
evaluation of lipid accumulation from lignocellulosic sugars by mortierella isabellina for biodiesel production.	the filamentous fungus mortierella isabellina atcc42613 was used to assess the conversion of different carbon sources (glucose, xylose, mixed glucose/xylose, acid and alkali treated corn stover hydrolysate) in submerged media to lipid. glucose and xylose cultures composed of varying initial sugar concentrations (28.1-91.7gl(-1), and 26.6-90.9gl(-1) respectively) showed a positive correlation to lipid accumulation, with significant quantities occurring at the upper limit of the substrate range (1 ...	2012	22330588
neighboring deschampsia flexuosa and trientalis europaea harbor contrasting root fungal endophytic communities.	fungal endophytic communities and potential host preference of root-inhabiting fungi of boreal forest understory plants are poorly known. the objective of this study was to find out whether two neighboring plant species, deschampsia flexuosa (poaceae) and trientalis europaea (primulaceae), share similar root fungal endophytic communities and whether the communities differ between two sites. the study was carried out by analysis of pure culture isolates and root fungal colonization percentages. a ...	2013	22592854
rapid screening and cultivation of oleaginous microorganisms.	oleaginous microbial strains were cultivated to identify the best oil-producing strain amongst yarrowia lipolytica (cgmcc 2.1398), lipomyces starkeyi (cgmcc 2.1608), rhodosporidium toruloides (cgmcc 2.1389), mortierella isabellina (cgmcc 3.3410), cunninghamella blakeleana (cgmcc 3.970), and mycobacterium qj311. a method for rapid determination of oil content and fatty acid composition was established to identify the optimum oil-producing strains. this method had a relative standard deviation of ...	2012	22611917
feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw.	lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. lignocellulosic biomass is a suitable feedstock for biofuel production due to its abundance and low value. however, very limited study has been performed on lipid production by culturing oleaginous fungi with lignocellulosic materials. thus, identification of filamentous fungal strains capable of utilizing lignocellulosic hydrolysates ...	2012	22824058
effects of external enzymes on the fermentation of soybean hulls to generate lipids by mortierella isabellina.	hydrolytic enzymes were evaluated on the lipid accumulation via an oleaginous fungal species, mortierella isabellina, cultivated on sugars released from soybean hulls. the weight loss of soybean hull, fungal growth, and lipid production were tested under different loads of hydrolytic enzymes. m. isabellina could not directly utilize cellulose and adding cellulase and β-glucosidase significantly increased the cell growth and oil accumulation of m. isabellina on soybean hulls. the highest weight l ...	2012	23054821
co-hydrolysis of lignocellulosic biomass for microbial lipid accumulation.	the herbaceous perennial energy crops miscanthus, giant reed, and switchgrass, along with the annual crop residue corn stover, were evaluated for their bioconversion potential. a co-hydrolysis process, which applied dilute acid pretreatment, directly followed by enzymatic saccharification without detoxification and liquid-solid separation between these two steps was implemented to convert lignocellulose into monomeric sugars (glucose and xylose). a factorial experiment in a randomized block desi ...	2013	23124976
oil production by mortierella isabellina from whey treated with lactase.	whey, a by-product of cheese manufacturing is rich in nutrients such as lactose, proteins, and mineral salts. the fungus mortierella isabellina was used for production of oil containing γ-linoleic acid (gla) during fermentation on deproteinized whey permeate (dp-wp) with and without lactase addition. the maximum oil concentration was 3.65 g/l in dp-whey (16.0% lactose) without enzyme treatment. treatment of dp-wp with lactase resulted in an increase in oil content to 17.13 g/l. palmitic (22.50-2 ...	2013	23201516
lignocellulosic biomass as a carbohydrate source for lipid production by mortierella isabellina.	various carbon sources including monosugars, disaccharides and carboxymethyl-cellulose (cmc) were used for single-cell oil production by the filamentous fungus mortierella isabellina. in addition, the inhibitory effects of lignocellulose-derived compounds (lignin aldehydes, furan aldehydes and weak acid) were investigated. c6 sugars were preferably used for growth compared to c5 sugars. cmc was not an usable substrate, implying the absence of a cellulase system in this fungus. lignin derivatives ...	2013	23201519
integration of sewage sludge digestion with advanced biofuel synthesis.	sewage sludge rich in carbohydrates and other nutrients could be a good feedstock for fuel/chemical production. in this study, fungal and engineered bacterial cultivations were integrated with a modified anaerobic digestion to accumulate fatty acids on sewage sludge. the anaerobic digestion was first adjusted to enable acetogenic bacteria to accumulate acetate. a fungus (mortierella isabellina) and an engineered bacterium (escherichia coli created by optimizing acetate utilization and fatty acid ...	2013	23399500
microbial lipid production from xylose by mortierella isabellina.	culture conditions including nitrogen source and concentration, xylose concentration, and inoculum level were evaluated for the effect on cell growth and lipid production of an oleaginous fungus, mortierella isabellina, grown on xylose. yeast extract and ammonium sulfate were found to be the best amongst the organic and inorganic nitrogen sources tested, respectively. subsequent combination of these two nitrogen sources at a nitrogen ratio of 1:1 further enhanced lipid production. the highest ce ...	2013	23434808
biosynthesis of gamma-linolenic acid and beta-carotene by zygomycetes fungi.	due to increasing demand for natural sources of both polyunsaturated fatty acids (pufas) and beta-carotene, 28 zygomycetes fungal soil isolates were screened for their potential to synthesize these biologically active compounds. although all fungi produced c18 pufas, only nine strains also formed beta-carotene. although actinomucor elegans ccf 3218 was the best producer of gamma-linolenic acid (gla) (251 mg/l), umbelopsis isabellina ccf 2412 was found to be the most valuable fungus because of th ...	2013	23625863
the olive mill wastewater as substrate for single cell oil production by zygomycetes.	the conversion of olive mill wastewater (omw) into high added value lipids containing polyunsaturated fatty acids (pufa), in parallel with a significant phenolic removal by selected strains of zygomycetes, is reported here for the first time. the growth of mortierella isabellina, mortierella ramanniana, cunninghamella echinulata, mucor sp., thamnidium elegans and zygorhynchus moelleri on solidified media was not significantly affected by the presence of omw used in the growth medium up to 50% (v ...	2014	24316440
genome sequence of the mucoromycotina fungus umbelopsis isabellina, an effective producer of lipids.	umbelopsis isabellina is a fungus in the subdivision mucoromycotina, many members of which have been shown to be oleaginous and have become important organisms for producing oil because of their high level of intracellular lipid accumulation from various feedstocks. the genome sequence of u. isabellina nbrc 7884 was determined and annotated, and this information might provide insights into the oleaginous properties of this fungus.	2014	24578268
improved lipid accumulation by morphology engineering of oleaginous fungus mortierella isabellina.	oleaginous fungi capable of accumulating a considerable amount of lipids are promising sources for lipid-based biofuel production. the specific productivities of filamentous fungi in submerged fermentation are often correlated with morphological forms. however, the relationship between morphological development and lipid accumulation is not known. in this study, distinct morphological forms of oleaginous fungus mortierella isabellina including pellets of different sizes, free dispersed mycelia, ...	2014	24668212
oleaginous fungal lipid fermentation on combined acid- and alkali-pretreated corn stover hydrolysate for advanced biofuel production.	a combined hydrolysis process, which first mixed dilute acid- and alkali-pretreated corn stover at a 1:1 (w/w) ratio, directly followed by enzymatic saccharification without ph adjustment, has been developed in this study in order to minimize the need of neutralization, detoxification, and washing during the process of lignocellulosic biofuel production. the oleaginous fungus mortierella isabellina was selected and applied to the combined hydrolysate as well as a synthetic medium to compare fung ...	2014	24768942
lipid profiling and corresponding biodiesel quality of mortierella isabellina using different drying and extraction methods.	four lipid extraction methods (bligh & dyer, hexane & isopropanol, dichloromethane & methanol, and hexane) were evaluated to extract lipid from freeze- and oven-dried fungus mortierella isabellina atcc42613. the highest lipid yield (41.8%) was obtained from bligh & dyer extraction on the oven-dried fungal biomass with a methanol:chloroform:water ratio of 2:1:0.8. other lipid extraction methods on both freeze- and oven-dried samples had lipid yields ranging from 20.7% to 35.9%. non-polar lipid wa ...	2014	25034797
microbial lipid production from corn stover via mortierella isabellina.	microbial lipid is a promising source of oil to produce biofuel if it can be generated from lignocellulosic materials. mortierella isabellina is a filamentous fungal species featuring high content of oil in its cell biomass. in this work, m. isabellina was studied for lipid production from corn stover. the experimental results showed that m. isabellina could grow on different kinds of carbon sources including xylose and acetate, and the lipid content reached to 35 % at c/n ratio of 20. with dilu ...	2014	25082770
acid protease production in fungal root endophytes.	fungal endophytes are ubiquitous in healthy root tissue, but little is known about their ecosystem functions, including their ability to utilize organic nutrient sources such as proteins. root-associated fungi may secrete proteases to access the carbon and mineral nutrients within proteins in the soil or in the cells of their plant host. we compared the protein utilization patterns of multiple isolates of the root endophytes phialocephala fortinii s.l., meliniomyces variabilis and umbelopsis isa ...	2015	25344260
a self-sustaining advanced lignocellulosic biofuel production by integration of anaerobic digestion and aerobic fungal fermentation.	high energy demand hinders the development and application of aerobic microbial biofuel production from lignocellulosic materials. in order to address this issue, this study focused on developing an integrated system including anaerobic digestion and aerobic fungal fermentation to convert corn stover, animal manure and food wastes into microbial lipids for biodiesel production. dairy manure and food waste were first anaerobically digested to produce energy and solid digestate (ad fiber). ad fibe ...	2015	25543542
effects of inhibitory compounds in lignocellulosic hydrolysates on mortierella isabellina growth and carbon utilization.	oleaginous fungus mortierella isabellina showed excellent lipid conversion on non-detoxified lignocellulosic hydrolysate. this study investigated the effects of inhibitory compounds (furfural, hydroxymethylfurfural, and ferulic and coumaric acids) in lignocellulosic hydrolysate on m. isabellina growth and lipid production. m. isabellina can tolerate furfural (∼1 g/l), hydroxymethylfurfural (∼2.5 g/l), ferulic (∼0.5 g/l) and coumaric acid (∼0.5 g/l) with normal growth rates. synergistic effect of ...	2015	25710679
unusual microbial lactonization and hydroxylation of asiatic acid by umbelopsis isabellina.	asiatic acid (1) is a natural triterpenoid isolated from centella asiatica. this paper reports the microbial transformation of asiatic acid by an endophytic fungus umbelopsis isabellina to obtain derivatives potentially useful for further studies. incubation of asiatic acid with u. isabellina afforded two derivatives 2α,3β,7β, 23-tetrahydroxyurs-12-ene-28-oic acid (2) and 2α,3β,7β,23-tetrahydroxyurs-11-ene-28,13-lactone (3). the structures of these compounds were elucidated by spectral data. com ...	2015	26194478
degradation and toxicity reduction of the endocrine disruptors nonylphenol, 4-tert-octylphenol and 4-cumylphenol by the non-ligninolytic fungus umbelopsis isabellina.	nonylphenol (np), 4-tert-octylphenol (4-t-op) and 4-cumylphenol (4-cp) are pollutants that are known as endocrine disruptors mainly due to their estrogen-mimicking activity. these phenolic substances are used in a wide range of industrial and commercial applications. in the present study, biodegradation of tnp, 4-t-op and 4-cp using the non-ligninolytic fungus umbelopsis isabellina was investigated. after 12h of incubation, more than 90% of initially applied tnp, 4-t-op and 4-cp (25mgl(-1)) were ...	2016	26492175
proteomic analysis of mortierella isabellina m6-22 during cold stress.	we aimed to gain a better understanding of cold adaption in mortierella isabellina m6-22 by using proteomics approaches. the temperature range and optimal temperature for m6-22 growth were investigated, and composition changes in fatty acids were analyzed. accompanied with the 2-d gel electrophoresis, maldi-tof/tof-ms analysis was conducted to characterize alterations in protein profiling in m6-22 cultured at 30 °c for 24 h and 15 °c for another 24 h when compared with those cultured at 30 °c fo ...	2016	27262947
single cell oil production by mortierella isabellina from steam exploded corn stover degraded by three-stage enzymatic hydrolysis in the context of on-site enzyme production.	single cell oil (sco), promising as alternative oil source, was produced from steam exploded corn stover (secs) by mortierella isabellina. different bioprocesses from secs to sco were compared and the bioprocess c using the three-stage enzymatic hydrolysis was found to be the most efficient one. the bioprocess c used the lowest enzyme input 20fpiu cellulase/g glucan and the shortest time 222h, but produced 44.94g dry cell biomass and 25.77g lipid from 327.63g dry secs. it had the highest lipid c ...	2016	27343451
exploring eukaryotic formate metabolisms to enhance microbial growth and lipid accumulation.	c1 substrates (such as formate and methanol) are promising feedstock for biochemical/biofuel production. numerous studies have been focusing on engineering heterologous pathways to incorporate c1 substrates into biomass, while the engineered microbial hosts often demonstrate inferior fermentation performance due to substrate toxicity, metabolic burdens from engineered pathways, and poor enzyme activities. alternatively, exploring native c1 pathways in non-model microbes could be a better solutio ...	2017	28149324