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genetic diversity and biological control activity of novel species of closely related pseudomonads isolated from wheat field soils in south australia.rhizobacteria closely related to two recently described species of pseudomonads, pseudomonas brassicacearum and pseudomonas thivervalensis, were isolated from two geographically distinct wheat field soils in south australia. isolation was undertaken by either selective plating or immunotrapping utilizing a polyclonal antibody raised against p. brassicacearum. a subset of 42 isolates were characterized by amplified 16s ribosomal dna restriction analysis (ardra), biolog analysis, and gas chromatog ...200010742249
pseudomonas brassicacearum sp. nov. and pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from brassica napus and arabidopsis thaliana.bacteria isolates phenotypically related to pseudomonas corrugata have frequently been isolated from the rhizosphere of arabidopsis thaliana and brassica napus grown on different soils. 16s rdna (rrs) gene sequencing, dna-dna hybridization, biochemical characterization and siderophore typing showed that these isolates belong to two different species that are distinct from other species of the genus pseudomonas, including p. corrugata. a description of properties of these two new species is given ...200010826782
utilization of mutants to analyze the interaction between arabidopsis thaliana and its naturally root-associated pseudomonas.a model system based on the arabidopsis thaliana (l.) heynh. ws ecotype and its naturally colonizing pseudomonas thivervalensis rhizobacteria was defined. pseudomonas strains colonizing a. thaliana were found to modify the root architecture either in vivo or in vitro. a gnotobiotic system using bacteria labelled with green fluorescent protein revealed that p. thivervalensis exhibited a colonization profile similar to that of other rhizobacterial species. mutants of a. thaliana affected in root h ...200111216839
pseudomonas kilonensis sp. nov., a bacterium isolated from agricultural soil.a total of 131 bacterial isolates related to pseudomonas corrugata were obtained from an agricultural soil from northern germany. based on 16s rrna gene sequences, pcr-based genome fingerprinting and multilocus enzyme electrophoresis, they formed two groups, a (119 strains) and b (12 strains). as members of each group were highly similar, a single strain of each group was subsequently characterized by a polyphasic taxonomic approach. the selected member of group a was identified as a strain of p ...200111491357
siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads.a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ...200212039729
transcriptome analysis of arabidopsis colonized by a plant-growth promoting rhizobacterium reveals a general effect on disease resistance.rna transcript levels of arabidopsis plants, infected by the rhizobacterium pseudomonas thivervalensis (strain mlg45), and axenic control plants were compared using cdna microarrays representing approximately 14 300 genes. the analysis revealed an increase of defence-related transcripts in the shoots of bacterized plants relative to control (axenic) plants. these modifications of transcript levels were confirmed by physiological experiments. plants infected with p. thivervalensis were more resis ...200314535883
characterization of acc deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of brassica napus.one hundred cu-resistant-endophytic bacteria were isolated from cu-tolerant plants grown on cu mine wasteland, of which, eight cu-resistant and 1-aminocyclopropane-1-carboxylate (acc) deaminase-producing endophytic bacteria were obtained based on the acc deaminase activity of the bacteria and characterized with respect to metal resistance, production of acc deaminase, indole-3-acetic acid (iaa) as well as siderophores and mineral phosphate solubilization. ralstonia sp. j1-22-2, pantoea agglomera ...201121315404
effects of root inoculation with bacteria on the growth, cd uptake and bacterial communities associated with rape grown in cd-contaminated soil.two metal-resistant and plant growth-promoting bacteria (burkholderia sp. j62 and pseudomonas thivervalensis y-1-3-9) were evaluated for their impacts on plant growth promotion, cd availability in soil, and cd uptake in rape (brassica napus) grown in different level (0, 50, and 100 mg kg(-1)) of cd-contaminated soils. the impacts of the bacteria on the rape-associated bacterial community structures were also evaluated using denaturing gradient gel electrophoresis (dgge) analysis of bacterial dna ...201323177252
btex biodegradation and its nitrogen removal potential by a newly isolated pseudomonas thivervalensis mah1.benzene, toluene, ethylbenzene, and xylene (btex) are of great environmental concern because of their widespread occurrence in groundwater and soil, posing an increasing threat to human health. the aerobic denitrifying btex-degrading bacterium pseudomonas thivervalensis mah1 was isolated from btex-contaminated sediment under nitrate-reducing conditions. the degradation rates of benzene, toluene, ethylbenzene, and xylene by strain mah1 were 4.71, 6.59, 5.64, and 2.59 mg·l⁻¹day⁻¹, respectively. th ...201526221863
analysis of bacterial communities associated with potting media.potting media are commonly used by growers in different parts of the world for potted plants, raising seedlings and for improving soil characteristics. this study was conducted to characterize bacterial communities occurring in 13 commercial potting media products originating from seven countries.201626844021
pyoverdine and histicorrugatin-mediated iron acquisition in pseudomonas thivervalensis.the genome of pseudomonas thivervalensis lmg 21626(t) has been sequenced and a genomic, genetic and structural analysis of the siderophore mediated iron acquisition was undertaken. pseudomonas thivervalensis produces two structurally new siderophores, pyoverdine pyothi which is typical for p. thivervalensis strains and a closely related strain, and the lipopeptidic siderophore histicorrugatin which is also detected in p. lini. histicorrugatin consists out of an eight amino acid long peptide whic ...201627007713
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