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repression of asexual embryogenesis in vitro by some plant growth regulators.a factor that represses asexual embryogenesis has been observed in the rutaceae, with particularly high concentrations in the naturally monoembryonic cultivars. this investigation was an initial step towards identifying the factor. citrus reticulata blanco ponkan mandarin nucellus explants and daucus carota l. 'queen anne's lace' callus were employed to examine effects of known plant growth regulators and to determine possible identity of one or more of them with the repressive factor. the chala ...1977145403
eponym: tangerine tonsils in tangier: high-density lipoprotein deficiency. 1978635625
[obstruction of the small intestine due to orange and tangerine]. 1976935939
[carboxypeptidases from the exocarp of mandarin orange (citrus unshiu marc.). i. purification and properties of two carboxypeptidases (author's transl)]. 19751240909
studies on natural antioxidants in citrus species. i. determination of antioxidative activities of citrus fruits.the antioxidative activities of twenty types of citrus fruits were investigated with a screening method which is based on rat liver microsomal lipid peroxidation induced by dihydronicotinamide adenine dinucleotide phosphate (nadph) and adenosine diphosphate (adp). the activities of the exocarp were greater than those of the sarcocarp and the activities from immature fruits (collected in july-august) were greater than those from mature fruits. the strongest antioxidative activity was found in pon ...19921394715
[a case of occupational asthma caused by arrowhead scale in mandarin orange-worker].a 50-year-old woman with occupational asthma, whose attacks were provoked by inhalation of arrowhead scale (unapsis yanonensis kuwana) attached to the leaves of mandarin oranges is reported. she experienced asthmatic attacks while picking leaves and harvesting mandarin oranges. because arrowhead scale-dust stuck to the leaves was suspected to be the allergen, tests of allergy were performed using an extract of the allergen prepared by unger's method in our laboratory. asthmatic attack was provok ...19921630051
[effect of temperature on the aerobic degradation of vitamin c in citric fruit juices].by means of the method of the 2,4-dinitrophenylhydrazine the total ascorbic acid content in lima, lemon, tangerine and grapefruit juices, fresh and kept at four temperatures and different times, was determined. it was confirmed that in all the cases, the aerobic degradation of ascorbic acid follows a kinetic first order and that the values of the reaction rate are different between species and even between varieties of lemon and tangerine. the values of the equation terms are reported, and examp ...19892490897
[pharmacognostic studies on the peel of citrus reticulata blanco cv. zhangshuensis and citrus reticulata blanco var. kinokuni (tanaka) h. h. hu produced in jiangxi].pharmacognostical studies on the peel of citrus reticulata cv. zhangshuensis and citrus reticulata var. kinokuni have been carried out in comparison with four crude drugs of chenpi. information on the research of resources of these two drugs is provided.19892597317
[an ultrasonographic picture of a growing pseudotumor in the abdominal cavity].the case history of patient is presented who was diagnosed a tumor in abdominal cavity in an ultrasonographic examination. in the following surgery the tumour was extirpated and histology verified a liposarcoma. the control ultrasonographic examination revealed a growing solid formation in the region of resection. for presumed relapse of liposarcoma the patient underwent laparotomy again. in the region of the original tumour there was a conglomerate of adhesions in a size of tangerine. the histo ...19892667770
[hepatic adenoma and oral contraceptives: personal case].a 38-year-old italian patient complained of dyspeptic disturbances and a feeling of postprandial swelling or weight. she had been taking oral contraceptives (ocs) for about 6 years. ultrasound showed a small rotund formation in the liver. surgery began and a tumor about the size of a tangerine was found and removed. after the surgery oc use was discontinued and she returned home totally healed on the 14th day after the surgery. the hypothesis on the association between benign liver tumors a ...19853004281
aflatoxin b1 production in orange (citrus reticulata) juice by isolates of aspergillus flavus link.out of 7 isolates of aspergillus flavus obtained from rotting orange (citrus reticulata) fruits, 3 isolates were found to be toxigenic, producing variable amounts of aflatoxin b1 on a semisynthetic liquid medium. the most toxigenic isolates were further evaluated in plain juice and juice supplemented either with 0.05% yeast extract or 0.5% sucrose or both, at three different incubation periods. the maximum yield of aflatoxin b1 (162.5 micrograms/25 ml) was obtained from the juice supplemented wi ...19873106811
[the chemical constituents of citrus reticulata]. 19853158433
simplified cleanup and gas chromatographic determination of organophosphorus pesticides in crops.a simple and efficient cleanup method for gas chromatographic determination of 23 organophosphorus pesticides in crops including onion is described. the sample was extracted with acetone. the extract was purified with coagulating solution, which contained ammonium chloride and phosphoric acid, and then filtered by suction. the filtrate was diluted with nacl solution and reextracted with benzene. the organic layer was evaporated and injected into a gas chromatograph equipped with a flame photomet ...19873610958
prevalence rates and risk factors for allergic symptoms among inhabitants in rural districts.in order to determine the prevalence of allergic disorders and their association with agricultural factors, a total of 3,717 inhabitants of rural districts in kumamoto prefecture were ashed to fill out a questionnaire concerning their allergic status. the results obtained were as follows: one or more items of allergic symptoms were reported by 44% of the subjects. the prevalence rate of each symptom was 8% for respiratory organs, 5% for delayed type of respiratory disorders, 10% for nasal mucosa ...19873613248
inhibition of carcinogenesis by some minor dietary constituents.previous work has shown that food contains a large number of minor dietary constituents that can inhibit the occurrence of cancer. additional inhibitors from four different natural sources will be the subject of this presentation. 1. citrus fruit oils. orange, tangerine, lemon, and grapefruit oils given p.o. induce increased glutathione (gsh) s-transferase activity in tissues of the mouse. when fed in the diet prior to and during the course of administration of benzo(a)pyrene (bp), the four citr ...19853916195
the enzymatic conversion of cis-(14c)phytofluene, trans-(14c)phytofluene, and trans-zeta-(14c)carotene to poly-cis-acyclic carotenes by a cell-free preparation of tangerine tomato fruit plastids. 19744208687
[epidemiologic survey on dermatitis from a new fungicide used in tangerine orchards in kumamoto prefecture]. 19684237752
the isolation, purification, and characterization of cis-zeta-carotene and the demonstration of its conversion to acyclic, monocyclic and dicyclic carotenes by a soluble enzyme system obtained from the plastids of tangerine tomato fruits. 19744831327
[bacterial growth on roots and near roots of the tangerine tree]. 19685735987
effect of preharvest spray of plant growth regulators on size, composition and storage behaviour of coorg mandarins [citrus reticulata (blanco]. 19665913173
the stereospecific biosynthesis of phytoene and polyunsaturated carotenes.1. the incorporation of [2-(14)c,(5r)-5-(3)h(1)]mevalonic acid and [2-(14)c,5-(3)h(2)]-mevalonic acid into phytoene, phytofluene, zeta-carotene, neurosporene, alpha-, beta-, gamma- and delta-carotene and lycopene by slices of fruit from two tomato mutants (delta and tangerine) and into alpha- and beta-carotene by bean leaves has been studied. 2. in the formation of phytoene, all the pro-r-hydrogen atoms from c-5 of mevalonic acid are retained whereas two pro-s-hydrogen atoms are lost. 3. possibl ...19676049923
an ultrastructural study of the mature chromoplasts of the tangerine tomato (lycopersicon esculentum var. "golden jubilee"). 19676080099
heterotopic bone formation in a lower transrectal abdominal incision.one case of a cicatricial hernia after appendectomi is presented where at operation it was found that the margins of the hernia opening in the abdominal wall consisted of an almost circular, rigid, bony formation with an inner diameter measuring 6 x 4.5 cm through which the hernia, the size of a tangerine, protruded.19806451121
determination of norflurazon residues in mixed crop matrices.a method for determining norflurazon residues in mixed crop matrices is described. the method included methanol soxhlet extraction, hexane wash, addition of 10% saline, methylene chloride extraction, concentration, preparative thin layer chromatography of 10% sample aliquot, and resuspension of sample zone for gas-liquid chromatographic analysis. the lower level of electron capture detection is 0.01 ppm. recoveries for 0.08 ppm spiked samples of cottonseed; soybean seed and foliage; peanut seed ...19817198114
[a preliminary study on bioactivity of orange and tangerine peel extracts against aphis and mites].an assay was made on the bioactivity of the extracts of tangerine peel from cinocitrus tangerina, orange peel from citrus sinensis and mixed tangerine peel from cinocitrus sp. against aphis semia phis heraclei, aphis craccivora, uroleucon gobonis and myzus persicae using residual film or topical method, and against mites tetranychus viennensis and t. trancatus using slide-dip or immersion method. test results show that these extracts have strong bioactivity against aphids and mites. the correcte ...19957576134
trombiculid fauna and seasonal abundance of leptotrombidium scutellare (acari: trombiculidae) in an endemic area of scrub typhus (tsutsugamushi disease) in yamakita town, kanagawa prefecture, japan.using the tullgren funnel method, we surveyed for trombiculid larvae around dwellings and in mandarin orange groves on the slopes of sengenyama and maruyama, yamakita town, kanagawa prefecture, japan, during the period between 8 october 1992 and 8 january 1993. in total, 6,690 trombiculid larvae, consisting of 6,669 leptotrombidium scutellare (nagayo, mitamura, tamiya & tenjin), 13 l. fuji kuwata, berge & philip, two l. himizu (sasa, kumada, hayashi, enomoto, fukuzumi & obata), two neotrombicula ...19947815396
[dermoid cysts of the oral floor. a clinical case report and review of the literature].the authors report two cases of dermoid cysts of the oral floor. the incidence of this pathology in this region is difficult to assess due to the rarity of reported cases. in particular, dermoid cysts appear to derive from the inclusion of epithelial cells on the median line in the fusion point between the first and second branchial arch. other pathogenetic causes are however reported in the literature, namely traumatic epidermal implants during fetal development in utero. dermoid cysts are most ...19948065288
double-barrelled tubular duplication of the ileum.a three year old boy with a 10 cm double-barrelled tubular duplication of the distal ileum is reported. he presented with a small bowel obstruction after a large meal of mandarin oranges. at laparotomy an obstruction of both lumens was found which communicated at both ends with the normal ileal lumen. although cystic duplications of the small intestine are common, tubular duplications are relatively rare, especially an obstruction with impacted undigested foods.19938240869
[32 cases of postoperative osteogenic sarcoma treated by chemotherapy combined with chinese medicinal herbs].32 cases of postoperative osteogenic sarcoma treated by chemotherapy combined with chinese medicinal herbs were compared with 26 similar cases as control group. the drugs used in chemotherapy consisted of two regimens, ddp and high-dose mtx plus vcr. the results showed that the side effects of chemotherapy in control group were consistent with literatures; while the group treated with chinese medicinal herbs suffered less toxic effects, the difference between two groups was statistically signifi ...19938339032
studies on the differentiation inducers of myeloid leukemic cells from citrus species.an attempt was made to isolate differentiation inducers from aurantii nobilis pericarpium and the fruit peel of citrus reticulata blanco (rutaceae). twenty-seven kinds of flavones, including five new flavones, were isolated after repeated chromatography from methanol extracts of these plants and their structures were established, from their physicochemical data, to be highly methoxylated flavones. each compound, except for two flavone glucosides, showed the differentiation inducing activity towa ...19938508474
beta-cryptoxanthin selectively increases in human chylomicrons upon ingestion of tangerine concentrate rich in beta-cryptoxanthin esters.beta-cryptoxanthin is a major source of vitamin a, often second only to beta-carotene, and is present in fruits such as oranges, tangerines, and papayas. here, we studied the uptake of this carotenoid upon ingestion of tangerine juice concentrate, rich in beta-cryptoxanthin esters. increasing amounts of free beta-cryptoxanthin were detected in chylomicrons and serum. peak levels in chylomicrons were reached at t = 6 h, and the concentration returned toward basal levels at t = 9 h. no beta-crypto ...19958554331
isolation of anti-leukemia compounds from citrus reticulata.in vitro effects of medicinal plant extracts from the pericarpium of citrus reticulata (cv jiao gan) (pcrj) on the growth and differentiation of a recently characterized murine myeloid leukemic cell clone wehi 3b (jcs) were investigated. extracts of pcrj not only inhibited the proliferation of jcs cells in a dose dependent manner, but also induced differentiation of jcs cells into macrophages and granulocytes. morphological differentiation of pcrj treated jcs cells was associated with an increas ...19968614280
exposure evaluation is a crucial step for quantitative risk assessment of methomyl.methomyl, methyl n-[[(methylamino)carbonyl]oxy]ethanimidothioate is a carbamate insecticide with anticholinesterase activity. as a broad spectrum insecticide, it is one of the most frequently used pesticides in tangerine orchards in thailand. although methomyl is said to be rapidly eliminated from experimental animals (1) high incidence of acute poisonings was reported among patients occupationally exposed to a powder formulation of methomyl (2, 3). in this passive dosimetry study of tangerine g ...19958645116
pool cue chalk: a source of environmental lead.lead compounds are used as coloring agents for numerous products. two cases of children with elevated blood lead concentrations encountered by the authors suggested that pool cue chalk may serve as a source of environmental lead. the objective of this study was to determine lead content of various brands and colors of pool cue chalk. atomic absorption analyses were conducted of 23 different types of pool cue chalk for lead content. three of 23 types of pool cue chalk contained more than 7000 ppm ...19968657539
major fruit and vegetable contributors to the main serum carotenoids in the spanish diet.to identify the main sources of serum carotenoids (lutein, zeaxanthin, lycopene, beta-cryptoxanthin, alpha-carotene and beta-carotene) among the fruits and vegetables in the spanish diet, to be used in the design of food questionnaires and to provide useful information for epidemiological and comparative studies.19968730612
influence of sample treatment on esr signal of irradiated citrus.esr spectra of the hard seed cover and kernel coating of irradiated orange and tangerine fruits were obtained under different sample drying conditions to analyze the effect of treatment on esr line at g = 2.0033 (line a). the spectra shows almost the same lines that appear in stalks, achenes, seeds and skins of fresh fruit. the peak-to-peak intensity of the line a of the spectra shows a linear variation with dose in the range studied (up to 5 kgy) under controlled sample preparation. q-band esr ...19969022204
limonoids from citrus reticulata and their moult inhibiting activity in mosquito culex quinquefasciatus larvae.three limonoids, namely limonin, nomilin and obacunone, were isolated from the seeds of citrus reticulata [blanco coorg mandarin]. their structures were confirmed by spectroscopic studies. with 4th instar larvae of mosquito culex quinquefasciatus, the ec50 for inhibition of adult emergence was 6.31, 26.61 and 59.57 ppm for obacunone, nomilin and limonin, respectively. the pattern of mortality at around the ec50 levels was indicative of moult inhibiting activity. the efficacy of a limonoid enrich ...19979115692
citrus flavonoids: a review of past biological activity against disease. discovery of new flavonoids from dancy tangerine cold pressed peel oil solids and leaves. 19989781298
patient appraisal of mandarin orange didanosine: implications for adherence and well-being.didanosine (videx, ddi) has been shown to be an effective agent in the treatment of hiv disease. however, patients have had difficulties adhering to didanosine preparations due to taste aversion and/or associated nausea. the efficacy of didanosine may, therefore, be compromised. this is of concern to clinicians who seek to promote patient adherence to medication regimes and to foster the quality of patient well-being. in this study, focus groups were used to gather exploratory data about patient ...19999934668
plasma and hepatic cholesterol and hepatic activities of 3-hydroxy-3-methyl-glutaryl-coa reductase and acyl coa: cholesterol transferase are lower in rats fed citrus peel extract or a mixture of citrus bioflavonoids.the cholesterol-lowering effects of tangerine peel extract and a mixture of two citrus flavonoids were tested. male rats were fed a 1 g/100 g high-cholesterol diet for 42 d with supplements of either tangerine-peel extract or a mixture of naringin and hesperidin (0.5 g/100 g) to study the effects of plasma and hepatic lipids, hepatic enzyme activities, and the excretion of fecal neutral sterols. both the tangerine-peel extract and mixture of two flavonoids significantly lowered the levels (mean ...199910356084
insertional mutagenesis and cloning of the genes required for biosynthesis of the host-specific ak-toxin in the japanese pear pathotype of alternaria alternata.the japanese pear pathotype of alternaria alternata causes black spot of japanese pear by producing a host-specific toxin known as ak-toxin. restriction enzyme-mediated integration (remi) mutagenesis was used to tag genes required for toxin biosynthesis. protoplasts of a wild-type strain were treated with a linearized plasmid along with the restriction enzyme used to linearize the plasmid. of 984 remi transformants recovered, three produced no detectable ak-toxin and lost pathogenicity on pear l ...199910432635
sulphorhodamine b assay for measuring proliferation of a pigmented melanocyte cell line and its application to the evaluation of crude drugs used in the treatment of vitiligo.a rapid 96-well plate assay using sulphorhodamine b (srb) protein stain for cell number has been adopted to screen herbs used in traditional treatments of vitiligo for substances capable of stimulating melanocyte proliferation. its applicability to melan-a cells, a mouse pigmented cell line, has been validated. srb assay produced good linearity up to 11 x 10(4) cells/well and interference by melanin present in the cells accounted for less than 10% of the total optical density readings. the intra ...199910433470
characterization of paprika (capsicum annuum) extract in orange juices by liquid chromatography of carotenoid profiles.the carotenoid pigment profiles of authentic pure orange juices from spain and florida and an industrial paprika (capsicum annuum) extract used for food coloring were obtained using reversed-phase liquid chromatography with a c18 packed column and an acetone/methanol/water eluent system. the procedure involving the carotenoid extraction is described. both retention times and spectral properties using photodiode array detection for characterization of the major carotenoids at 430 and 519 nm are g ...199910552400
influence of epicuticular waxes on the photolysis of pirimicarb in the solid phase.the influence of epicuticular waxes extracted from different fruits on the photodegradation of pirimicarb (i) in the solid phase was studied. waxes were extracted with chcl(3) and chcl(3)/ch(3)oh from nectarines (n), oranges (o(r)), and mandarin oranges (m). all of the waxes affect the qualitative behavior of the photodegradation of i: the formation of photoproducts n-formylpirimicarb (ii) and demethylpirimicarb (iii) was hindered. this influence was found to be independent of the light sources ...199810554311
kuwait's total diet study: dietary intake of organochlorine, carbamate, benzimidazole and phenylurea pesticide residues.the state of kuwait in cooperation with the u.s. food and drug administration (fda) conducted a total diet study (tds) to estimate intakes of pesticide residues by the population. the levels of organochlorine (oc) pesticides, carbamates, benzimidazoles, and phenylureas in the tds core list are reported here. the tds core list was established through a national food consumption survey. all food items (140 for the kuwaiti adult) were prepared as eaten and analyzed for the pesticides mentioned abov ...200810589497
local anaesthetic activity of the essential oil of lavandula angustifolia.in this work we studied the local anaesthetic activity of the essential oil obtained from lavandula angustifolia mill., a medicinal plant traditionally used as an antispasmodic. we compared its activity to the essential oils obtained from two citrus fruits, citrus reticulata blanco and citrus limon (l.) burm. f., which have no medical uses. biological tests were also performed on the major pure components of l. angustifolia mill. essential oil: linalol and linalyl acetate as determined by gc and ...199910630108
preserved foods in relation to risk of nasopharyngeal carcinoma in shanghai, china.a population-based case-control study was conducted in shanghai, china, to investigate the association between dietary factors and risk of nasopharyngeal carcinoma (npc). the study included 935 npc patients aged 15 to 74 years and 1,032 community controls. exposures to salted fish and other protein-containing preserved food were associated with increased risk of npc. individuals who ate salted fish at least once a week had an 80% increase in risk of npc relative to those who ate salted fish less ...200010652427
fluorescence detection of proteins in sodium dodecyl sulfate-polyacrylamide gels using environmentally benign, nonfixative, saline solution.sypro tangerine stain is an environmentally benign alternative to conventional protein stains that does not require solvents such as methanol or acetic acid for effective protein visualization. instead, proteins can be stained in a wide range of buffers, including phosphate-buffered saline or simply 150 mm nacl using an easy, one-step procedure that does not require destaining. stained proteins can be excited by ultraviolet light of about 300 nm or with visible light of about 490 nm. the fluores ...200010726749
[effect of processing and storage time on content of hesperidin in green tangerine peel].the contents of hesperidin in green tangerine peel (citrus reticulata), in its processed products and in commecial samples were determined by hplc. the results indicate that the contents of hesperidin in the processed products from chengdu and huangyan are 14.28% and 10.75% less than shose of the crude drugs respectively. it has been found out that the content of hesperidin in green tangerine peel before and after processing reduces with the passage of storage time.199710743184
lack of correlation between in vitro and in vivo studies on the effects of tangeretin and tangerine juice on midazolam hydroxylation.tangeretin is a flavonoid that stimulates the catalytic activity of cytochrome p450 3a4 (cyp3a4) and is found in high levels in tangerine juice.200010801247
amounts and variation in grapefruit juice of the main components causing grapefruit-drug interaction.a method for the determination of three furocoumarins containing two new chemicals (gf-i-1 and gf-i-4) in commercially available grapefruit juice and grapefruit itself was developed using high-performance liquid chromatography (hplc). these components isolated from grapefruit juice have 5-geranyloxyfurocoumarin dimer structures showing extremely high affinities for a form of cytochrome p450 (cyp3a4). considerable differences were observed on the contents among commercial brands and also batches. ...200010872589
determination of vanillin in orange, grapefruit, tangerine, lemon, and lime juices using gc-olfactometry and gc-ms/ms.the presence of vanillin in orange, tangerine, lemon, lime, and grapefruit juices has been identified and confirmed using high-resolution gc retention index values, mass spectra, and aroma quality. the impact of vanillin on the flavor score for grapefruit juice is discussed and reported to be minimal. vanillin concentrations are determined to be in the low parts-per-million range for the various citrus juices. the calculated concentrations in the orange, tangerine, lemon, lime, and grapefruit ju ...200010898641
latin american food sources of carotenoids.latin america has a wide variety of carotenogenic foods, notable for the diversity and high levels of carotenoids. a part of this natural wealth has been analyzed. carrot, red palm oil and some cultivars of squash and pumpkin are sources of both beta-carotene and alpha-carotene. beta-carotene is the principal carotenoid of the palm fruits burití, tucumã and bocaiuva, other fruits such as loquat, marolo and west indian cherry, and sweet potato. buriti also has high amounts of alpha-carotene and g ...199910971848
adsorption studies on citrus reticulata (fruit peel of orange): removal and recovery of ni(ii) from electroplating wastewater.the ability of fruit peel of orange to remove zn, ni, cu, pb and cr from aqueous solution by adsorption was studied. the adsorption was in the order of ni(ii)>cu(ii)>pb(ii)>zn(ii)>cr(ii). the extent of removal of ni(ii) was found to be dependent on sorbent dose, initial concentration, ph and temperature. the adsorption follows first-order kinetics. the process is endothermic showing monolayer adsorption of ni(ii), with a maximum adsorption of 96% at 50 degrees c for an initial concentration of 5 ...200011040390
antibacterial activity of citrus reticulata peel extracts.citrus peels were successively extracted with hexane, chloroform and acetone using a soxhlet extractor. the hexane and chloroform extracts were fractionated into alcohol-soluble and alcohol-insoluble fractions. these fractions were tested against different gram positive and gram negative bacteria. the etoh-soluble fraction was found to be most effective. fractionation of etoh-soluble fraction on silica gel column yielded three polymethoxylated flavones, namely desmethylnobiletin, nobiletin and t ...200011204182
suppressive effects of citrus fruits on free radical generation and nobiletin, an anti-inflammatory polymethoxyflavonoid.citrus fruit intake is known to be associated with a reduction of cancer incidence. free radicals, including superoxide (o2-) and nitric oxide (no), are involved in some epithelial carcinogenesis processes. in the present study, we screened thirty-one citrus fruits for their suppressive activities toward three lines of free radical generating systems: 1) o2- generation by the xanthine (xa)-xanthine oxidase (xod) system; 2) o2- generation induced by 12-o-tetradecanoylphorbol-13-acetate (tpa) in d ...200011216485
inhibitors of platelet lipoxygenase from ponkan fruit.an activity-guided separation for inhibitors of rat platelet 12-lipoxygenase led to the isolation of two compounds, 4-o-feruloyl-5-o-caffeoylquinic acid (ic50; 5.5 microm) and methyl 4-o-feruloyl-5-o-caffeoylquinate (ic50; 1.9 microm) from the peel of ponkan fruit (citrus reticulata). the complete structure of each phenolic ester was determined by nmr spectroscopy [1h and 13c nmr spectra, 1h-1h correlation spectroscopy (cosy), 1h-detected heteronuclear multiple quantum coherence (hmqc), and hete ...200111314960
mass spectrometry compatibility of two-dimensional gel protein stains.as proteomic technology evolves, protein staining sensitivity is constantly being improved, enabling researchers to better visualize the proteome of their system. the current challenge is to balance the limits of detection of protein visualization with those of the mass spectrometric methods. in this report, mass spectra generated from human serum or rat liver proteins stained with either colloidal coomassie blue, daiichi silver, sypro orange, sypro red, sypro ruby, or sypro tangerine are compar ...200111332759
mycotic aneurysm of the carotid bifurcation in the neck: case report and review of the literature.mycotic aneurysms of the extracranial carotid artery are rare and difficult to diagnose. a search of the world literature published since 1966 reveals at least six cases of mycotic carotid aneurysms due to a salmonella septicemia. we present an exceptional case of mycotic pseudoaneurysm of the bifurcation of the carotid artery due to salmonella septicemia and discuss the pathogenesis as well as various aspects of the diagnosis and surgical management.200111334284
citrus juice composition does not influence radiation sensitivity of salmonella enteritidis.food substrate chemistry is known to influence radiation sensitivity of pathogenic bacteria. the sensitivity of a citrus juice outbreak strain of salmonella enteritidis to gamma radiation was determined in five commercial orange juice formulations. the juices differed in ph (3.87 to 4.13), calcium concentration (2.1 versus 36.9 mm), juice composition (orange versus orange-tangerine blend), and antioxidant power (11,751 to 12,826 microm ferric reducing-antioxidant power units). the dgamma (dose r ...200111403141
citrus pectin: characterization and inhibitory effect on fibroblast growth factor-receptor interaction.this study was undertaken to characterize the pectin from four citrus species and to determine their in vitro inhibitory activities on the binding of fibroblast growth factor (fgf) to the fgf receptor (fgfr). pectin from various parts of lemon, grapefruit, tangerine, and orange were isolated and characterized. tangerine had the highest pectin content among the four citrus species. segment membrane contained as much as or more pectin than flavedo/albedo. anhydrogalacturonic content was highest in ...200111410008
phenols in citrus peel byproducts. concentrations of hydroxycinnamates and polymethoxylated flavones in citrus peel molasses.in addition to the main flavanone glycosides (i.e., hesperidin and naringin) in citrus peel, polymethoxylated flavones and numerous hydroxycinnamates also occur and are major phenolic constituents of the molasses byproduct generated from fruit processing. although a small number of the hydroxycinnamates in citrus occur as amides, most occur as esters and are susceptible to alkaline hydrolysis. this susceptibility to alkaline hydrolysis was used in measuring the concentrations of hydroxycinnamate ...200111453761
analysis of lycopene geometrical isomers in biological microsamples by liquid chromatography with coulometric array detection.methods of analysis for determining low quantities of lycopene cis-trans isomers in biological tissues are needed. development of two liquid chromatography (lc) methods based on the polymeric c30 stationary phase equipped with coulometric electrochemical array detection (ed) is described. separation of 13 lycopene isomers including prolycopene, (a novel tetra-cis-lycopene found in tangerine tomatoes) was accomplished with both isocratic and gradient methods using different proportions of methano ...200111530988
[two complementary dominant genes controlling apomixis in genus citrus and poncirus].apomixis is very common in citrus genus and its related genus. two monoembryonic tangerine varieties (c. reticulata blanco) clementine and wilking were used as seed parents to cross with four polyembryonic orange varieties [c. sinensis (l.) osbeck]. both sexual and apomictic progenies were found in each f1 population with different segregation ratios. in a total of 74 wilking progenies, 23 were monoembryonic and 51 polyembryonic and the ratio of sexual and apomictic progenies was about 1:2 teste ...200111725641
tangerine dreams: cloning of carotenoid isomerase from arabidopsis and tomato. 200211884674
cloning of tangerine from tomato reveals a carotenoid isomerase essential for the production of beta-carotene and xanthophylls in plants.carotenoid biosynthesis in plants has been described at the molecular level for most of the biochemical steps in the pathway. however, the cis-trans isomerization of carotenoids, which is known to occur in vivo, has remained a mystery since its discovery five decades ago. to elucidate the molecular mechanism of carotenoid isomerization, we have taken a genetic map-based approach to clone the tangerine locus from tomato. fruit of tangerine are orange and accumulate prolycopene (7z,9z,7'z,9'z-tetr ...200211884678
contact dermatitis from the essential oil of tangerine in fragrance. 200211918605
histometrics: improvement of the dynamic range of fluorescently stained proteins resolved in electrophoretic gels using hyperspectral imaging.most image-based analyses, using absorbance or fluorescence of the spatial distribution of identifiable structures in complex biological systems, use only a very small number of dimensions of possible spectral data for the generation and interpretation of the image. we here extend the concepts of hyperspectral imaging, being developed in remote sensing, into analytical biotechnology. the massive volume of information contained in hyperspectral spectroscopic images requires multivariate analysis ...200111922594
differential inhibition of human cancer cell proliferation by citrus limonoids.limonoids have been shown to inhibit the growth of estrogen receptor-negative and -positive human breast cancer cells in culture. the primary objective of this study was to test the antiproliferative activity of limonoids (obacunone 17 beta-d-glucopyranoside, nomilinic acid 17 beta-d-glucopyranoside, limonin, nomilin, and a limonoid glucoside mixture), found in high concentrations in mandarin (citrus reticulata blanco), against a series of human cancer cell lines. the human cancer cell lines inc ...200111962254
[inheritance of organelle genomes of the somatic hybrid between cleopatra mandarin (citrus reticulata) and flying dragon (poncirus trifoliata)].cleaved amplified polymorphic sequence (caps) was successfully applied to analyze the organelle composition of three eight-year-old trees of the somatic hybrid between cleopatra mandarin (citrus reticulata) and flying dragon (poncirus trifoliata). five chloroplast and five mitochondrial universal primer pairs were used. all chloroplast primer pairs (rbcl-rbcl, rbcl-psa i, trnh-trnk, trnd-trnt, trnk-trnk) and three (nad 1 exon b-nad 1 exon c, 18s rrna-5s rrna, nad 4 exon 1-nad 4 exon 2) of the fi ...200211985273
a conditionally dispensable chromosome controls host-specific pathogenicity in the fungal plant pathogen alternaria alternata.the filamentous fungus alternaria alternata contains seven pathogenic variants (pathotypes), which produce host-specific toxins and cause diseases on different plants. previously, the gene cluster involved in host-specific ak-toxin biosynthesis of the japanese pear pathotype was isolated, and four genes, named akt genes, were identified. the akt homologs were also found in the strawberry and tangerine pathotypes, which produce af-toxin and act-toxin, respectively. this result is consistent with ...200212019223
determination of hesperidin and synephrine in pericarpium citri reticulatae by capillary electrophoresis with electrochemical detection.a method based on capillary electrophoresis with electrochemical detection (ce-ed) has been developed for the determination of hesperidin (hp) and synephrine (sp) in the chinese traditional herbal drug, pericarpium citri reticulatae, the dried rind of the ripe fruits of citrus reticulata blanco (mandarin orange). the effects of some important factors such as the acidity and concentration of running buffer, separation voltage, and detection potential were investigated to determine the optimum con ...200212043020
[dependence of activities of polysaccharide hydrolases and oxidases from cerrena unicolor on the source of carbon and aromatic acids in culture media].the activities of carboxymethylcellulase and xylanase in the higher basidial fungus cerrena unicolor grown in avicel-containing medium reached 1.95 and 1.50 units per mg protein, respectively, whereas in mannitol-containing medium they ranged from 0.02 to 0.05 units per mg protein. the activity of fungal beta-glucosidase depended on the carbon source in the culture medium and ranged from 2.1 units per mg protein in the presence of mannitol to 17.3 units per mg protein in the presence of avicel. ...200212068574
polyphenol antioxidants in citrus juices: in vitro and in vivo studies relevant to heart disease.it is well known that eating fruits and vegetables lowers the risk of chronic diseases such as heart disease and cancer. the question of what is/are the active ingredient(s) is still unresolved. the initial hypothesis was that the antioxidant vitamins were responsible. however, recently the polyphenols have been investigated since they have been found to have beneficial properties such as being strong antioxidants. we measured the polyphenol content of citrus juices by an oxidation-reduction col ...200212083455
inheritance of organelle dna sequences in a citrus-poncirus intergeneric cross.many land plants deviate from the maternal pattern of organelle inheritance. in this study, heterologous mitochondrial and chloroplast probes were used to investigate the inheritance of organelle genomes in the progeny of an intergeneric cross. the seed parent was lb 1-18 (a hybrid of citrus reticulata blanco cv. clementine x c. paradisi macf. cv. duncan) and the pollen parent was the cross-compatible species poncirus trifoliata (l.) raf. all 26 progeny examined exhibited maternal inheritance of ...200212195032
[studies on chemical constituents of processed green tangerine peel].to study the chemical constituents of processed green tangerine peel(citrus reticulata).200012212096
evaluation of filamentous fungi and inducers for the production of endo-polygalacturonase by solid state fermentation.aspergillus oryzae cct 3940, aspergillus awamori nrrl 3112 and a trichoderma sp.) were compared for their capacity to produce endo-polygalacturonase (endo-pg) in solid state fermentation. maximum pectinolytic activity was reached in 72 h of growth, the best two fungal strains being a. niger t0005007-2 and a. oryzae cct 3940. three types of commercial purified pectin and four of unprocessed pectin (tangerine, orange, tahiti lime and sweet lime rind) were used to assess the effect of pectin on the ...200212240994
diversity of endophytic bacterial populations and their interaction with xylella fastidiosa in citrus plants.citrus variegated chlorosis (cvc) is caused by xylella fastidiosa, a phytopathogenic bacterium that can infect all citrus sinensis cultivars. the endophytic bacterial communities of healthy, resistant, and cvc-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. the endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (dgge). dominant isolates were characterized by fat ...200212324338
frequent intake of tropical fruits that are rich in beta-cryptoxanthin is associated with higher plasma beta-cryptoxanthin concentrations in costa rican adolescents.dietary tocopherols and carotenoids may play a role in preventing cancer and cardiovascular diseases. because these may begin to develop during adolescence, dietary patterns during this period could influence long-term risk. the objective of this study was to examine the intake and plasma concentrations of the major carotenoids and tocopherols in 159 adolescents (mean +/- sd, 15.5 +/- 2.5 y old) living in costa rica. all participants completed a 135-item food-frequency questionnaire and provided ...200212368412
carotenoid isomerase: a tale of light and isomers.carotenoids are terpenoid pigments containing systems of conjugated double bonds, each of which can exist in a cis or trans configuration. the existence of a carotenoid isomerase (crtiso) mediating cis-to-trans isomerization has been suspected ever since the description of the tomato tangerine mutant in the early 1940s, whose fruits accumulate a poly-cis-isomer of lycopene. recently, crtiso has been cloned in synechocystis and in plants, and has been shown to be related to bacterial carotene des ...200212399171
is there meaningful plant resistance to diaprepes abbreviatus (coleoptera: curculionidae) in citrus rootstock germplasm?host plant resistance to the diaprepes root weevil, diaprepes abbreviatus (l.) was assessed for seedlings of 54 poncirus trifoliata (l.) raf. selections and two families of progeny from crosses between citrus and p. trifoliata. weight gain was consistently lower when larvae were reared in pots containing the progeny of citrus reticulata blanco 'sunki' x p. trifoliata 'flying dragon' compared with larvae reared on progeny of 'pearl' (c. reticulata x c. paradisi macf.) x 'flying dragon'. this is t ...200212403435
high-speed characterization and analysis of orange oils with tandem-column stop-flow gc and time-of-flight ms.high-speed gc with time-of-flight (tof) ms detection is used for the characterization and analysis of oils rendered from the peel of five diverse species of orange including bergemot orange, bitter orange, tangerine, mandarin orange, and sweet orange. with a user-defined signal-to-noise threshold of 100, 44 peaks were found and 36 compounds identified in the various oils. some major constituent components show large concentration ranges over the five species. a 14-m-long, 0. 18-mm-i.d. column en ...200212433099
increased growth of young citrus trees under reduced radiation load in a semi-arid climate.this study investigated the effects of radiation heat-load reduction by shading on the growth and development of citrus trees in a warm subtropical region. the experiment was conducted from mid-june until late october when daily maximal air temperature averaged 29.3 degrees c. two-year-old de-fruited murcott tangor (citrus reticulata blancoxcitrus sinensis (l.) osb.) trees were grown under 30% or 60% shade tunnels, or 60% flat shade (providing midday shade only), using highly reflective aluminiz ...200312493865
[studies on the chemical constituents of citrus reticulata].four compounds were isolated from the pericarp of citrus reticulata and three of them were identified as natsudaidai, nobiletin and 3,5,6,7,8,3',4'-heptamethoxyflavone. natsudaidai and 3,5,6,7,8,3',4'-heptamethoxyflavone were obtained from this plant for the first time.199812567525
a case of intestinal obstruction due to phytobezoar--an alternative surgical approach.we report a 5-year-old patient with phytobezoar mimicking acute appendicitis preoperatively. during laparotomy, it was detected that terminal ileum was obstructed by several fragments of rubbery material. bezoar was milked into the large bowel, and phytobezoar including tangerine residues was evacuated via appendix stump because of severe distended cecum, and high risk of the anastomotic leakage and intraperitoneal contamination following enterotomy of the inflamated and ischemic ileum. postoper ...200312661431
limonoids from citrus reticulata.the seeds of citrus reticulata afforded the new limonoid derivative, isolimonexic acid methyl ether, in addition to the previously isolated limonin, deacetylnomilin, obacunone and ichangin. the structure elucidation was achieved primarily through 1d and 2-d-nmr analyses. the marginal antimalarial activity of isolimonexic acid methyl ether is reported.200312710721
effects of carbohydrate starvation on gene expression in citrus root.the roots of alternate-bearing citrus (murcott, a citrus reticulata hybrid) trees undergo extreme fluctuations of carbohydrate abundance and starvation. using this system, we investigated the effect of root carbohydrate (total soluble sugar, sucrose and starch) depletion on carbohydrate-related gene expression. a series of genes, including those coding for starch phosphorylase ( stph-l and stph-h), adp-glucose pyrophosphorylase, small subunit ( agps), r1, plastidic adp/atp transporter ( aatp), p ...200312721844
leaf chlorophyll, net gas exchange and chloroplast ultrastructure in citrus leaves of different nitrogen status.one-year-old 'cleopatra mandarin' (citrus reticulata blanco) seedlings were raised in a greenhouse and fertilized with nitrogen (n) at four application frequencies. nitrogen-deficient leaves (86 mmol n m-2) had less chlorophyll per unit area, but a greater chlorophyll a:b ratio than n-fertilized leaves (> 187 mmol n m-2). leaf dry mass per area (dm area-1) and total chlorophyll concentration increased linearly with increasing leaf n, whereas chlorophyll a:b ratio declined. net assimilation of co ...200312730047
natural enemies of three mealybug species (hemiptera: pseudococcidae) found on citrus and effects of some insecticides on the mealybug parasitoid coccidoxenoides peregrinus (hymenoptera: encyrtidae) in south africa.the population density of mealybug species in some south african citrus orchards has increased to pest status in recent years. the characterization of the natural enemy complex and quantification of their contribution to the control of planococcus citri (risso), pseudococcus longispinus (targioni-tozzetti) and pseudococcus calceolariae (maskell) on citrus limon (l.) and citrus reticulata (blanco) was investigated through intensive sampling. eight primary and four secondary parasitoids, and two p ...200312762866
girdling affects carbohydrate-related gene expression in leaves, bark and roots of alternate-bearing citrus trees.effects of girdling on carbohydrate status and carbohydrate-related gene expression in citrus trees were investigated. alternate-bearing 'murcott' (a citrus reticulata hybrid of unknown origin) trees were girdled during autumn (25 sep. 2001) and examined 10 weeks later. girdling brought about carbohydrate (soluble sugar and starch) accumulation in leaves and shoot bark above the girdle, in trees during their fruitless, 'off' year. trees during their heavy fruit load, 'on' year did not accumulate ...200312763756
a tangerine-scented social odour in a monogamous seabird.social odours, conspecific chemical signals, have been demonstrated in every class of vertebrate except birds. the apparent absence is surprising, as every bird examined has a functional olfactory system and many produce odours. the crested auklet (aethia cristatella), a monogamous seabird, exhibits a distinctive tangerine-like scent closely associated with courtship. using t-maze experiments, we tested whether auklets preferred conspecific odours and whether they distinguished between different ...200312965022
the preparation and evaluation of tangerine peel tincture and tangerine syrup. 195413142943
similarity of host responses elicited by polysaccharides of animal and plant origin and by bacterial endotoxins.ten polysaccharides, isolated from various animal and plant sources, were selected for comparison with 2 bacterial polysaccharides, typical of gram-negative endotoxins. the tissue sources were: mouse (kidney, liver, lung, stomach, sarcoma 37, and carcinoma 241-6); rabbit skin and chick embryo skin; and tangerine and bryonia root. the bacterial endotoxins were those of s. typhosa and serr. marcescens. their relative potency was determined in inducing the following host effects: fever, tolerance t ...195713439117
a survey of genes differentially expressed during long-term heat-induced chilling tolerance in citrus fruit.long-term storage at low, non-freezing, temperature (1.5 degrees c) induces chilling injury in fruit of fortune mandarin (citrus clementina hort. ex tanaka x citrus reticulata, blanco), manifested as pitting and brown depressed areas that may end up with local cell death. pre-conditioning of fruit for 3 days at 37 degrees c prevented chilling injury. the use of suppression subtractive hybridization permitted the isolation of genes differentially expressed in heat-conditioned fruit exposed to chi ...200313680227
heat resistance & destruction rates of saccharomyces cerevisiae in mandarin orange (citrus reticulata blanco) juice. 196214040769
native and introduced host plants of anastrepha fraterculus and ceratitis capitata (diptera: tephritidae) in northwestern argentina.wild or commercially grown, native and exotic fruit were collected in 30 localities in the tucumán province (nw argentina) from january 1990 to december 1995 to determine their status as hosts of anastrepha fraterculus (wiedemann) and/or ceratitis capitata (wiedemann), the only two fruit fly species of economic and quarantine importance in argentina. a total of 84,094 fruit (3,466.1 kg) representing 33 species (7 native and 26 exotic) in 15 plant families were sampled. we determined the followin ...200314503581
effect of extraction method on the concentrations of selected bioactive compounds in mandarin juice.a mandarin-type citrus fruit, ponkan (citrus reticulata), was processed by in-line, chopper pulper, and hand-press extractions to investigate the effect of extraction method on the concentrations of bioactive compounds in processed juice. concentrations of polymethoxylated flavones (tangeretin, nobiletin, and sinensetin) and beta-cryptoxanthin in juice, and inhibitory activities against arachidonate cyclooxygenase and lipoxygenases of the juice extract were analyzed. the juice processed by hand- ...200314640582
effect of the rootstock and interstock grafted in lemon tree (citrus limon (l.) burm.) on the flavonoid content of lemon juice.the grafting of the rootstock with the lemon tree is an agronomical technique used to improve production and/or quality of the fruit. the interstock has been used with different fruit trees to modulate the tree size, fruit production and quality, and the aging of the tree. the lemon trees grafted with interstocks increase their longevity, lemon production and quality; interstocks are also used to decrease the thickness of the trunk at the grafting point. this enlarging of the trunk provokes a de ...200414733516
evidence that dietary supplementation with carotenoids and carotenoid-rich foods modulates the dna damage: repair balance in human lymphocytes.epidemiological evidence has shown that the habitual consumption of diets high in fruits and vegetables is associated with reduced risk of cancers. the challenge is to identify causal mechanisms of effect. the aim of the current study was to determine whether an increase in rate of removal of dna single-strand breaks (ssb) following oxidative challenge could be provoked ex vivo in peripheral blood lymphocytes (pbl). the pbl were isolated from apparently healthy volunteers following dietary inter ...200414748939
brevipalpus californicus, b. obovatus, b. phoenicis, and b. lewisi (acari: tenuipalpidae): a review of their biology, feeding injury and economic importance.the genus brevipalpus includes most of the economically important species of tenuipalpidae. many brevipalpus species reproduce by theletokous parthenogenesis while other species reproduce by male fertilization of female eggs. previous researchers have determined that brevipalpus californicus (banks), b. obovatus donnadieu, and b. phoenicis (geijskes) females were haploid with two chromosomes. the life cycle and developmental times for these three species are reviewed. longevity of each brevipalp ...200314756411
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