Publications

recognition of two new species of intestinal spirochetes: serpulina intermedia sp. nov. and serpulina murdochii sp. nov.	on the basis of dna-dna hybridization data, nine intestinal spirochete strains were grouped into five genospecies. three of these genospecies were previously recognized serpulina species, serpulina hyodysenteriae (type strain, b78), serpulina innocens (type strain, b256), and serpulina pilosicoli (type strain, p43/6/78; previously "anguillina coli"). the other two genospecies were found to be new serpulina species, for which we propose the names serpulina intermedia sp. nov. (with type strain pw ...	1997	9336899
evaluation of day-old specific pathogen-free chicks as an experimental model for pathogenicity testing of intestinal spirochaete species.	specific pathogen-free chicks aged 1 day were challenged per os with strains of five different species of intestinal spirochaete originally isolated from pigs or human beings. a virulent strain of serpulina hyodysenteriae (wa 15) colonized chicks, causing retarded growth rate and histological changes, including caecal atrophy, epithelial and goblet cell hyperplasia, and crypt elongation. a further strain of s. hyodysenteriae (sa3), which was apparently avirulent for pigs, and a strain of serpuli ...	1998	9651813
serpulina alvinipulli sp. nov., a new serpulina species that is enteropathogenic for chickens.	strain c1t is an anaerobic spirochaete that causes intestinal disease in chickens. multilocus enzyme electrophoresis analysis and 16s rrna sequence comparisons have indicated that this spirochaete is a serpulina strain. in these investigations, various phenotypic and genomic properties useful for establishing a taxonomic identity for strain c1t were studied. as determined by electron microscopy, cells of the spirochaete measured 8-11 x 0.22-0.34 mum and had a typical spirochaete ultrastructure. ...	1998	9734021
isolation of serpulina murdochii from the joint fluid of a lame pig.		1999	10028395
identification of porcine intestinal spirochetes by pcr-restriction fragment length polymorphism analysis of ribosomal dna encoding 23s rrna.	the brachyspira (formerly serpulina) species rrl gene encoding 23s ribosomal rna (rrna) was used as a target for amplification of a 517bp dna fragment by polymerase chain reaction (pcr). the primers for pcr amplification had sequences that were conserved among brachyspira 23s rrna gene and were designed from nucleotide sequences of brachyspira hyodysenteriae, serpulina intermedia, brachyspira innocens and brachyspira pilosicoli available from the genbank database. digestion of pcr-generated prod ...	2000	10889409
cloning and dna sequence analysis of an immunogenic glucose-galactose mglb lipoprotein homologue from brachyspira pilosicoli, the agent of colonic spirochetosis.	colonic spirochetosis (cs) is a newly emerging infectious disease of humans and animals caused by the pathogenic spirochete brachyspira (formerly serpulina) pilosicoli. the purpose of this study was to characterize an antigen that was recognized by antibodies present in sera of challenge-exposed pigs. the gene encoding the antigen was identified by screening a plasmid library of human b. pilosicoli strain sp16 (atcc 49776) genomic dna with hyperimmune and convalescent swine sera. the predicted a ...	2000	10899855
identification of brachyspira hyodysenteriae-specific dna fragments using representational difference analysis.	two novel brachyspira hyodysenteriae-specific dna fragments, designated as bh100 and bh400, were identified using representational difference analysis. to isolate the fragments the combined dna of the brachyspira pilosicoli, brachyspira intermedia, brachyspira murdochii and brachyspira innocens reference strains was subtracted from the genome of b. hyodysenteriae strain b204. both fragments were present in a single copy and mapped to different positions on the genome of b. hyodysenteriae b78(t). ...	2002	12044671
the use of multilocus enzyme electrophoresis to characterise intestinal spirochaetes (brachyspira spp.) colonising hens in commercial flocks.	multilocus enzyme electrophoresis (mlee) was used to identify, examine genetic relationships and look at disease associations of a collection of 53 intestinal spirochaete isolates previously recovered from the faeces of adult hens on 14 farms in qld, australia. the mlee results were compared with those previously obtained using species-specific pcr amplifications. the isolates were divided into five brachyspira species groups by mlee: brachyspira murdochii (n=17), b. intermedia (n=15), b. pilosi ...	2005	15795087
assessment of diagnostics and antimicrobial susceptibility testing of brachyspira species using a ring test.	there is no ring test for quality assessment available in europe for diagnostics and antimicrobial susceptibility testing of the fastidious, anaerobic bacteria of the genus brachyspira. therefore, an international ring test for brachyspira spp. was performed once a year during 2002-2004. two sets of coded samples were prepared and distributed on each occasion. one set comprised six swabs dipped in pig faeces spiked with brachyspira spp. intended for diagnostics. the other set comprised two pure ...	2005	15982833
amplification and sequencing of brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in austrian pigs shows frequent solitary presence of brachyspiramurdochii.	brachyspira infections are significant causes of enterocolitis in pigs. in order to differentiate pathogenic species (brachyspira (br.) hyodysenteriae, brachyspira pilosicoli) from less pathogenic or non-pathogenic species (brachyspira intermedia, brachyspira innocens, brachyspira murdochii) in paraffin-embedded tissue samples a polymerase chain reaction (pcr) protocol allowing identification of brachyspira at species level in archival material was developed. this approach was complemented by se ...	2005	16213113
the prevalences of brachyspira spp. and lawsonia intracellularis in swedish piglet producing herds and wild boar population.	the aim of the present study was to survey the prevalences of the enteric pathogens brachyspira hyodysenteriae, brachyspira pilosicoli and lawsonia intracellularis in swedish growing pigs and in the swedish wild boar population and to relate these findings to clinical signs. the study included 105 randomly selected herds, constituting approximately one third of swedish herds with a herd size of >100 sows. the herds were located all over the country. in these herds, growth promoters were not used ...	2005	16283917
reclassification of serpulina intermedia and serpulina murdochii in the genus brachyspira as brachyspira intermedia comb. nov. and brachyspira murdochii comb. nov.	a reassessment was undertaken of published characteristics of the five species of anaerobic intestinal spirochaetes in the genus brachyspira (brachyspira aalborgi, brachyspira alvinipulli, brachyspira hyodysenteriae, brachyspira innocens and brachyspira pilosicoli) and the two species in the genus serpulina (serpulina intermedia and serpulina murdochii). comparisons were made of published descriptions, including phenotypic properties, grouping by multilocus enzyme electrophoresis, dna base compo ...	2006	16627646
distribution of the clpx gene in brachyspira species and reactivity of recombinant brachyspira pilosicoli clpx with sera from mice and humans.	previously, a clpx gene encoding a predicted 67 kda membrane-associated atpase subunit of the clp protease (clpx) was identified in a porcine strain (95/1,000) of the intestinal spirochaete brachyspira pilosicoli. in the current study, the distribution of this large clpx gene was investigated in a collection of strains representing all seven brachyspira spp. using pcr with internal primers, an 878 bp portion of the gene was detected in 29 of 35 strains (83 %) of b. pilosicoli, 6 of 24 strains (2 ...	2007	17577058
prevalence, disease associations and risk factors for colonization with intestinal spirochaetes (brachyspira spp.) in flocks of laying hens in north-eastern italy.	the present study investigated the occurrence of anaerobic intestinal spirochaetes of the genus brachyspira in laying hen flocks in treviso province, north-eastern italy, with respect to prevalence, spirochaete species present, disease associations and risk factors for colonization. a total of 450 faecal samples from 45 sheds on 29 laying hen farms were cultured for intestinal spirochaetes. nineteen sheds on 12 farms contained chickens with symptoms consistent with avian intestinal spirochaetosi ...	2008	18568654
brachyspira murdochii colitis in pigs.	the weakly beta-hemolytic porcine spirochete brachyspira murdochii is considered a normal intestinal commensal. in the present study, however, a field case of b murdochii-associated catarrhal colitis was identified in a pig, as characterized by extensive spirochetal colonization of the surface epithelium. experimentally, 8 weaned pigs were challenged with the b murdochii isolate, reproducing catarrhal colitis in 2 animals. by applying fluorescent in situ hybridization using a species-specific ol ...	2010	20173181
the complete genome sequence of the pathogenic intestinal spirochete brachyspira pilosicoli and comparison with other brachyspira genomes.	the anaerobic spirochete brachyspira pilosicoli colonizes the large intestine of various species of birds and mammals, including humans. it causes "intestinal spirochetosis", a condition characterized by mild colitis, diarrhea and reduced growth. this study aimed to sequence and analyse the bacterial genome to investigate the genetic basis of its specialized ecology and virulence.	2010	20625514
complete genome sequence of brachyspira murdochii type strain (56-150).	brachyspira murdochii stanton et al. 1992 is a non-pathogenic, host-associated spirochete of the family brachyspiraceae. initially isolated from the intestinal content of a healthy swine, the 'group b spirochaetes' were first described as serpulina murdochii. members of the family brachyspiraceae are of great phylogenetic interest because of the extremely isolated location of this family within the phylum 'spirochaetes'. here we describe the features of this organism, together with the complete ...	2010	21304710
identification of a new steroid degrading bacterial strain h5 from the baltic sea and isolation of two estradiol inducible genes.	the presence of steroid hormones in the aquatic environment is potentially threatening the population dynamics of all kinds of sea animals and public health. environmental estrogens in water have been reported to be associated with abnormal sexual development and abnormal feminizing responses in some animals. new approaches for the bioremediation of steroid hormones from the environment are therefore urgently sought. we have previously isolated a steroid degrading bacterial strain (h5) from the ...	2011	21310233
typing of brachyspira spp. from rodents, pigs and chickens on swedish farms.	the aim of the current study was to look for evidence of possible cross-species transmission of brachyspira species between rodents and farm animals. to do this, previously collected and characterised brachyspira isolates from rodents, pigs and chickens on the same farms were analysed by random amplified polymorphic dna (rapd). isolates with similar rapd banding patterns were further typed by pulsed-field gel electrophoresis (pfge). identical isolates of brachyspira pilosicoli, brachyspira inter ...	2011	21497461
antimicrobial susceptibility of brachyspira spp. isolated from commercial laying hens and free-living wild mallards (anas platyrhynchos).	in vitro antimicrobial susceptibility to tylosin, valnemulin, tiamulin, doxycycline, lincomycin and ampicillin was investigated by broth dilution in 48 brachyspira spp. isolates from commercial laying hens (n=30) and free-living wild mallards (anas platyrhynchos) (n=18). presumed pathogens (brachyspira alvinipulli, brachyspira intermedia, brachyspira pilosicoli), commensals (brachyspira murdochii, brachyspira innocens, "brachyspira pulli"), and isolates of undetermined species affiliation were i ...	2011	21812718
species characterization and minimum inhibitory concentration patterns of brachyspira species isolates from swine with clinical disease.	typhlocolitis and dysentery due to brachyspira hyodysenteriae infection represent an economically important disease syndrome in growing pigs. largely disappearing from u.s. swine herds in the late 1990 s and early 2000s, brachyspira-associated disease and bacterial isolation from swine with clinical disease has increased in the last several years, and non-b. hyodysenteriae isolates are commonly identified. antimicrobial resistance has been demonstrated in brachyspira spp. isolates from europe an ...	2011	22362794
occurrence of viable brachyspira spp. on carcasses of spent laying hens from supermarkets.	brachyspira spp. are frequent inhabitants of the chicken's intestine and some have been associated with enteric disease in humans. we studied contamination with brachyspira spp. of carcasses of spent laying hens as a possible source of infections for humans and animals that may eat this meat. eleven batches of hen carcasses, for a total of 110 carcasses, were bought in belgian supermarkets during 2009-2010. carcass rinse samples were examined for the presence of brachyspira. brachyspira spp. wer ...	2012	22986196
identification of weakly haemolytic brachyspira isolates recovered from pigs with diarrhoea in spain and portugal and comparison with results from other countries.	weakly haemolytic anaerobic intestinal spirochaetes of the genus brachyspira are commonly identified based on species-specific gene sequences. apart from the pathogenic brachyspira pilosicoli, the distribution and disease associations of the other weakly haemolytic brachyspira species in pigs have not been comprehensively investigated. in this study weakly haemolytic brachyspira isolates (n=67) from spanish and portuguese pigs with diarrhoea, negative in a routine diagnostic pcr for b. pilosicol ...	2013	23928181
"brachyspira hampsonii" clade i isolated from belgian pigs imported to germany.	this report describes the detection of "brachyspira (b.) hampsonii" clade i in belgian pigs imported to germany. two of seventeen pigs from one herd were reported positive for brachyspira hyodysenteriae by culture in a belgian diagnostic laboratory, but negative for this brachyspira species by specific pcr. in this study, from 22 fecal samples and 2 colon contents of these animals various brachyspira species were cultured and identified by nox-rflp as brachyspira murdochii, brachyspira innocens ...	2014	24332829
matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid identification of brachyspira species isolated from swine, including the newly described "brachyspira hampsonii".	the brachyspira species traditionally associated with swine dysentery and other diarrheal diseases in pigs are brachyspira hyodysenteriae, brachyspira pilosicoli, and, to a lesser extent, brachyspira murdochii. "brachyspira hampsonii" is a recently proposed novel species that causes clinical disease similar to that caused by b. hyodysenteriae. matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) systems are increasingly available in veterinary diagnostic la ...	2014	25012082
antimicrobial susceptibility patterns of brachyspira species isolated from swine herds in the united states.	outbreaks of swine dysentery, caused by brachyspira hyodysenteriae and the recently discovered "brachyspira hampsonii," have reoccurred in north american swine herds since the late 2000s. additionally, multiple brachyspira species have been increasingly isolated by north american diagnostic laboratories. in europe, the reliance on antimicrobial therapy for control of swine dysentery has been followed by reports of antimicrobial resistance over time. the objectives of our study were to determine ...	2016	27252458