Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| recognition of two new species of intestinal spirochetes: serpulina intermedia sp. nov. and serpulina murdochii sp. nov. | on the basis of dna-dna hybridization data, nine intestinal spirochete strains were grouped into five genospecies. three of these genospecies were previously recognized serpulina species, serpulina hyodysenteriae (type strain, b78), serpulina innocens (type strain, b256), and serpulina pilosicoli (type strain, p43/6/78; previously "anguillina coli"). the other two genospecies were found to be new serpulina species, for which we propose the names serpulina intermedia sp. nov. (with type strain pw ... | 1997 | 9336899 |
| evaluation of day-old specific pathogen-free chicks as an experimental model for pathogenicity testing of intestinal spirochaete species. | specific pathogen-free chicks aged 1 day were challenged per os with strains of five different species of intestinal spirochaete originally isolated from pigs or human beings. a virulent strain of serpulina hyodysenteriae (wa 15) colonized chicks, causing retarded growth rate and histological changes, including caecal atrophy, epithelial and goblet cell hyperplasia, and crypt elongation. a further strain of s. hyodysenteriae (sa3), which was apparently avirulent for pigs, and a strain of serpuli ... | 1998 | 9651813 |
| serpulina alvinipulli sp. nov., a new serpulina species that is enteropathogenic for chickens. | strain c1t is an anaerobic spirochaete that causes intestinal disease in chickens. multilocus enzyme electrophoresis analysis and 16s rrna sequence comparisons have indicated that this spirochaete is a serpulina strain. in these investigations, various phenotypic and genomic properties useful for establishing a taxonomic identity for strain c1t were studied. as determined by electron microscopy, cells of the spirochaete measured 8-11 x 0.22-0.34 mum and had a typical spirochaete ultrastructure. ... | 1998 | 9734021 |
| scanning electron microscopy and fluorescent in situ hybridization of experimental brachyspira (serpulina) pilosicoli infection in growing pigs. | two groups of six 8-week-old pigs were challenged with 1x10(9) cfu brachyspira (serpulina) pilosicoli or serpulina intermedia daily for 3 consecutive days to study the pathology of porcine colonic spirochetosis by scanning electron microscopy (sem) and fluorescent in situ hybridization (fish) with oligonucleotide probes targeting ribosomal rna specific for b. pilosicoli and the genus brachyspira/serpulina. six pigs served as noninoculated controls. the animals were euthanatized successively betw ... | 2000 | 10643977 |
| evaluation of a 23s rdna polymerase chain reaction assay for identification of serpulina intermedia, and strain typing using pulsed-field gel electrophoresis. | a polymerase chain reaction assay, amplifying a 1027 base pair portion of the 23s rdna gene, was evaluated for identification of the intestinal spirochaete serpulina intermedia. a total of 34 strains of s. intermedia isolated from pigs and chickens and 195 strains of other related species were tested. the optimised assay correctly identified all the s. intermedia strains, but generated 11 false positive reactions, giving a test sensitivity of 100% and a test specificity of 94.3%. the false posit ... | 2000 | 10665541 |
| detectability and prevalence of brachyspira species in herds rearing health class feeder pigs in finland. | faeces samples were taken three times at two-week intervals, from the farrowing units of four herds of known brachyspira (formerly serpulina) status and one of unknown brachyspira status. brachyspira hyodysenteriae, brachyspira pilosicoli, brachyspira intermedia and brachyspira group iii were isolated from the faecal samples from the weaners in the herds using either a maximum of 50 ppm of olaquindox or no feed additives. the detection rates were relatively consistent. however, b hyodysenteriae ... | 2000 | 10777042 |
| identification of porcine intestinal spirochetes by pcr-restriction fragment length polymorphism analysis of ribosomal dna encoding 23s rrna. | the brachyspira (formerly serpulina) species rrl gene encoding 23s ribosomal rna (rrna) was used as a target for amplification of a 517bp dna fragment by polymerase chain reaction (pcr). the primers for pcr amplification had sequences that were conserved among brachyspira 23s rrna gene and were designed from nucleotide sequences of brachyspira hyodysenteriae, serpulina intermedia, brachyspira innocens and brachyspira pilosicoli available from the genbank database. digestion of pcr-generated prod ... | 2000 | 10889409 |
| cloning and dna sequence analysis of an immunogenic glucose-galactose mglb lipoprotein homologue from brachyspira pilosicoli, the agent of colonic spirochetosis. | colonic spirochetosis (cs) is a newly emerging infectious disease of humans and animals caused by the pathogenic spirochete brachyspira (formerly serpulina) pilosicoli. the purpose of this study was to characterize an antigen that was recognized by antibodies present in sera of challenge-exposed pigs. the gene encoding the antigen was identified by screening a plasmid library of human b. pilosicoli strain sp16 (atcc 49776) genomic dna with hyperimmune and convalescent swine sera. the predicted a ... | 2000 | 10899855 |
| prevalence of intestinal pathogens in danish finishing pig herds. | our aim was to determine the prevalence of the intestinal bacteria: lawsonia intracellularis, brachyspira hyodysenteriae, serpulina intermedia, brachyspira innocens, brachyspira pilosicoli, pathogenic escherichia coli (serogroups o138, o139, o141 and o149) and salmonella enterica in danish finishing pig herds. a total of 79 herds was randomly selected and visited during 1998. from each herd, 20 faecal samples were collected from individual pigs weighing 30-50kg. furthermore, 10 pooled pen sample ... | 2000 | 10960714 |
| risk factors for intestinal pathogens in danish finishing pig herds. | the objective of this investigation was to identify risk factors for infection with the intestinal bacteria: lawsonia intracellularis, brachyspira hyodysenteriae, serpulina intermedia, brachyspira innocens, brachyspira pilosicoli and swine-pathogenic escherichia coli (serogroups o138, o139, o141 and o149) in danish finishing pig herds.a total of 79 herds was randomly selected and visited during 1998. from each herd, 20 faecal samples were collected from individual pigs weighing 30-50 kg. in tota ... | 2001 | 11448502 |
| intestinal spirochete infections of chickens: a review of disease associations, epidemiology and control. | this paper presents an overview of intestinal spirochete infections of chickens. it focuses particularly on studies in australia, where recent surveys of 136 layer and broiler breeder flocks have revealed a high rate of infection (>40%) with intestinal spirochetes. infection was not detected in broiler flocks. approximately 50% of isolates from infected flocks were brachyspira (serpulina) intermedia or b. pilosicoli, with the other isolates being b. innocens, b. murdochii or the proposed species ... | 2001 | 11708751 |
| dietary enzyme and zinc bacitracin reduce colonisation of layer hens by the intestinal spirochaete brachyspira intermedia. | brachyspira intermedia strain hb60 was used to experimentally infect 40 individually caged 22-week-old laying hens. another 10 control birds were sham-inoculated with sterile broth. all chickens received an experimental layer diet based on wheat. the infected birds were randomly divided into four groups of 10, with the diet for each group containing either 50 ppm zinc bacitracin (znb), 100 ppm znb, 256 ppm of dietary enzyme (avizyme), 1302), or no additive. birds were kept for 6 weeks after infe ... | 2002 | 11955785 |
| identification of brachyspira hyodysenteriae-specific dna fragments using representational difference analysis. | two novel brachyspira hyodysenteriae-specific dna fragments, designated as bh100 and bh400, were identified using representational difference analysis. to isolate the fragments the combined dna of the brachyspira pilosicoli, brachyspira intermedia, brachyspira murdochii and brachyspira innocens reference strains was subtracted from the genome of b. hyodysenteriae strain b204. both fragments were present in a single copy and mapped to different positions on the genome of b. hyodysenteriae b78(t). ... | 2002 | 12044671 |
| influence of in-feed zinc bacitracin and tiamulin treatment on experimental avian intestinal spirochaetosis caused by brachyspira intermedia. | thirty individually caged layer hens were inoculated with brachyspira intermedia, and 20 control birds remained unchallenged. birds received a diet containing 100 parts/10(6) zinc bacitracin (znb), and were monitored for 10 weeks. b. intermedia was recovered sporadically from five of the inoculated birds, and there were no significant effects on body weight, faecal water or egg production. znb was presumed to be indirectly inhibiting spirochaete growth, and when removed from the diet, 18 of the ... | 2002 | 12396352 |
| survival of intestinal spirochaete strains from chickens in the presence of disinfectants and in faeces held at different temperatures. | this study aimed to evaluate the efficacy of some commonly used disinfectants in inactivating the pathogenic avian intestinal spirochaetes brachyspira intermedia and brachyspira pilosicoli, and to examine spirochaete survival in chicken caecal faeces held at 4 degrees c, 25 degrees c or 37 degrees c. six disinfectants were evaluated at their recommended working concentrations: alkaline salts, quaternary ammonium, iodine as an iodophor, chlorine from a chlorine-release agent, glutaraldehyde and h ... | 2003 | 14676016 |
| brachyspira hyodysenteriae and other strongly beta-haemolytic and indole-positive spirochaetes isolated from mallards (anas platyrhynchos). | the aims of the current study were to collect intestinal spirochaetes (genus brachyspira) from farmed and wild mallards (anas platyrhynchos) and to identify and classify those isolates that phenotypically resembled brachyspira hyodysenteriae, an enteric pathogen of pigs. the isolation rate of brachyspira spp. was high from both farmed (93 %) and wild mallards (78 %). in wild mallards, it appeared that brachyspira spp. were more likely to be found in migratory birds (multivariate analysis: rr = 1 ... | 2004 | 15017285 |
| a wheat-based diet enhances colonization with the intestinal spirochaete brachyspira intermedia in experimentally infected laying hens. | this study investigated the influence of feeding diets based on cereals with different non-starch polysaccharide content on colonization with the intestinal spirochaete brachyspira intermedia in experimentally infected laying hens. the diets were based on wheat, barley, or barley and sorghum, all with or without supplementation with exogenous dietary enzymes. six groups of 12 individually caged laying hens were fed the respective diets for 2 weeks, and then challenged at 20 weeks of age by crop ... | 2004 | 15370044 |
| a cross-sectional study to investigate the occurrence and distribution of intestinal spirochaetes (brachyspira spp.) in three flocks of laying hens. | a cross-sectional study was conducted on a commercial egg-producing farm with a history of wet litter. a total of 600 fresh caecal faecal samples were obtained from under cages of laying hens in three sheds each containing flocks of approximately 5400 hens. samples were cultured for intestinal spirochaetes, and growth on the primary isolation plate was observed under a phase contrast microscope and subjected to pcrs specific for the intestinal spirochaetes brachyspira intermedia and brachyspira ... | 2004 | 15708815 |
| the wheat variety used in the diet of laying hens influences colonization with the intestinal spirochaete brachyspira intermedia. | this study investigated whether feeding different wheat varieties to laying hens could influence colonization with the intestinal spirochaete brachyspira intermedia. fifty isa-brown laying hens were divided into two groups. one group were fed a laying-hen diet formulated with wheat variety westonia, and one were fed the diet incorporating variety stilleto. each group was divided into 15 hens experimentally infected with b. intermedia and 10 uninfected controls. the 30 infected hens were housed i ... | 2004 | 15763727 |
| assessment of diagnostics and antimicrobial susceptibility testing of brachyspira species using a ring test. | there is no ring test for quality assessment available in europe for diagnostics and antimicrobial susceptibility testing of the fastidious, anaerobic bacteria of the genus brachyspira. therefore, an international ring test for brachyspira spp. was performed once a year during 2002-2004. two sets of coded samples were prepared and distributed on each occasion. one set comprised six swabs dipped in pig faeces spiked with brachyspira spp. intended for diagnostics. the other set comprised two pure ... | 2005 | 15982833 |
| amplification and sequencing of brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in austrian pigs shows frequent solitary presence of brachyspiramurdochii. | brachyspira infections are significant causes of enterocolitis in pigs. in order to differentiate pathogenic species (brachyspira (br.) hyodysenteriae, brachyspira pilosicoli) from less pathogenic or non-pathogenic species (brachyspira intermedia, brachyspira innocens, brachyspira murdochii) in paraffin-embedded tissue samples a polymerase chain reaction (pcr) protocol allowing identification of brachyspira at species level in archival material was developed. this approach was complemented by se ... | 2005 | 16213113 |
| the prevalences of brachyspira spp. and lawsonia intracellularis in swedish piglet producing herds and wild boar population. | the aim of the present study was to survey the prevalences of the enteric pathogens brachyspira hyodysenteriae, brachyspira pilosicoli and lawsonia intracellularis in swedish growing pigs and in the swedish wild boar population and to relate these findings to clinical signs. the study included 105 randomly selected herds, constituting approximately one third of swedish herds with a herd size of >100 sows. the herds were located all over the country. in these herds, growth promoters were not used ... | 2005 | 16283917 |
| antimicrobial susceptibility testing of brachyspira intermedia and brachyspira pilosicoli isolates from australian chickens. | susceptibilities of predominantly australian isolates of the pathogenic intestinal spirochaetes brachyspira intermedia (n = 25) and brachyspira pilosicoli (n = 17) from chickens were tested in agar dilution against four concentrations each of the antimicrobials tiamulin, lincomycin, tylosin, metronidazole, tetracycline and ampicillin. based on available minimum inhibitory concentration (mic) breakpoint values for brachyspira hyodysenteriae or other gram-negative enteric veterinary pathogens, iso ... | 2006 | 16448937 |
| reclassification of serpulina intermedia and serpulina murdochii in the genus brachyspira as brachyspira intermedia comb. nov. and brachyspira murdochii comb. nov. | a reassessment was undertaken of published characteristics of the five species of anaerobic intestinal spirochaetes in the genus brachyspira (brachyspira aalborgi, brachyspira alvinipulli, brachyspira hyodysenteriae, brachyspira innocens and brachyspira pilosicoli) and the two species in the genus serpulina (serpulina intermedia and serpulina murdochii). comparisons were made of published descriptions, including phenotypic properties, grouping by multilocus enzyme electrophoresis, dna base compo ... | 2006 | 16627646 |
| development of a two-step nested duplex pcr assay for the rapid detection of brachyspira pilosicoli and brachyspira intermedia in chicken faeces. | avian intestinal spirochaetosis (ais) is an infection of the caeca and/or colo-rectum of laying and meat breeder hens caused by anaerobic intestinal spirochaetes of the genus brachyspira. ais can result in a variety of symptoms, including delayed and/or reduced egg production, and increased faecal water content. the two most commonly reported brachyspira species involved in ais are brachyspira pilosicoli and brachyspira intermedia, and their detection and identification can be difficult and time ... | 2006 | 16677782 |
| distribution of the clpx gene in brachyspira species and reactivity of recombinant brachyspira pilosicoli clpx with sera from mice and humans. | previously, a clpx gene encoding a predicted 67 kda membrane-associated atpase subunit of the clp protease (clpx) was identified in a porcine strain (95/1,000) of the intestinal spirochaete brachyspira pilosicoli. in the current study, the distribution of this large clpx gene was investigated in a collection of strains representing all seven brachyspira spp. using pcr with internal primers, an 878 bp portion of the gene was detected in 29 of 35 strains (83 %) of b. pilosicoli, 6 of 24 strains (2 ... | 2007 | 17577058 |
| prevalence, disease associations and risk factors for colonization with intestinal spirochaetes (brachyspira spp.) in flocks of laying hens in north-eastern italy. | the present study investigated the occurrence of anaerobic intestinal spirochaetes of the genus brachyspira in laying hen flocks in treviso province, north-eastern italy, with respect to prevalence, spirochaete species present, disease associations and risk factors for colonization. a total of 450 faecal samples from 45 sheds on 29 laying hen farms were cultured for intestinal spirochaetes. nineteen sheds on 12 farms contained chickens with symptoms consistent with avian intestinal spirochaetosi ... | 2008 | 18568654 |
| vaccination with an autogenous bacterin fails to prevent colonization by brachyspira intermedia in experimentally infected laying chickens. | avian intestinal spirochaetosis (ais) is a disease complex affecting adult laying and breeding chickens associated with infection by anaerobic intestinal spirochaetes of the genus brachyspira. options for control of ais are limited, as few effective antimicrobial agents are registered for use in laying chickens. one of the two most commonly encountered pathogenic species in ais is b. intermedia, and the aim of the current study was to determine whether a b. intermedia bacterin vaccine would help ... | 2009 | 18760550 |
| identification of genes associated with prophage-like gene transfer agents in the pathogenic intestinal spirochaetes brachyspira hyodysenteriae, brachyspira pilosicoli and brachyspira intermedia. | vsh-1 is an unusual prophage-like gene transfer agent (gta) that has been described in the intestinal spirochaete brachyspira hyodysenteriae. the gta does not self-propagate, but it assembles into a virus-like particle and transfers random 7.5kb fragments of host dna to other b. hyodysenteriae cells. to date the gta vsh-1 has only been analysed in b. hyodysenteriae strain b204, in which 11 late function genes encoding prophage capsid, tail and lysis elements have been described. the aim of the c ... | 2009 | 18950961 |
| brachyspira intermedia strain diversity and relationships to the other indole-positive brachyspira species. | the aims of this study were to use multilocus sequence typing (mlst) to (i) investigate the population structure, diversity and molecular epidemiology of the weakly haemolytic anaerobic intestinal spirochaete brachyspira intermedia, and (ii) determine the relationship of the species to the other two indole-positive but strongly haemolytic brachyspira species--b. hyodysenteriae and "b. suanatina". seventy-seven b. intermedia isolates from pigs and chickens were analysed, with the nucleotide seque ... | 2010 | 19944544 |
| brachyspira intermedia and brachyspira pilosicoli are commonly found in older laying flocks in pennsylvania. | anaerobic intestinal spirochetes (genus brachyspira) include several species that are recognized as pathogens of poultry. surveys undertaken in europe and australia have shown that layer and breeder flocks are often colonized by the pathogenic species brachyspira intermedia and brachyspira pilosicoli, but similar surveys have not been conducted in the united states. in the current study, dna was extracted from fecal samples (n=50) collected from each of 21 flocks of laying hens >40 wk of age in ... | 2009 | 20095153 |
| faecal excretion of intestinal spirochaetes by urban dogs, and their pathogenicity in a chick model of intestinal spirochaetosis. | this study aimed to obtain information about the types of spirochaetes colonising urban dogs in thailand, and to investigate their pathogenic potential in a day-old chick model of intestinal spirochaetosis. spirochaetes were isolated from the faeces of six of 47 (12.8%) healthy dogs and 11 of 104 (10.6%) dogs with diarrhoea. their biochemical properties and 16s ribosomal dna sequences were analysed. four isolates were identified as brachyspira pilosicoli, three resembled "brachyspira pulli", nin ... | 2011 | 21320714 |
| typing of brachyspira spp. from rodents, pigs and chickens on swedish farms. | the aim of the current study was to look for evidence of possible cross-species transmission of brachyspira species between rodents and farm animals. to do this, previously collected and characterised brachyspira isolates from rodents, pigs and chickens on the same farms were analysed by random amplified polymorphic dna (rapd). isolates with similar rapd banding patterns were further typed by pulsed-field gel electrophoresis (pfge). identical isolates of brachyspira pilosicoli, brachyspira inter ... | 2011 | 21497461 |
| antimicrobial susceptibility pattern of brachyspira intermedia isolates from european layers. | a broth microdilution method was used to determine the antimicrobial susceptibility of 20 brachyspira intermedia isolates obtained from different layer flocks in belgium and the netherlands between 2008 and 2010. the antimicrobial agents used were tylosin, tilmicosin, tiamulin, valnemulin, doxycycline, and lincomycin. the minimal inhibitory concentration (mic) distribution patterns of tylosin, tilmicosin, lincomycin, and doxycycline were bimodal, demonstrating acquired resistance against doxycyc ... | 2011 | 21663534 |
| antimicrobial susceptibility of brachyspira spp. isolated from commercial laying hens and free-living wild mallards (anas platyrhynchos). | in vitro antimicrobial susceptibility to tylosin, valnemulin, tiamulin, doxycycline, lincomycin and ampicillin was investigated by broth dilution in 48 brachyspira spp. isolates from commercial laying hens (n=30) and free-living wild mallards (anas platyrhynchos) (n=18). presumed pathogens (brachyspira alvinipulli, brachyspira intermedia, brachyspira pilosicoli), commensals (brachyspira murdochii, brachyspira innocens, "brachyspira pulli"), and isolates of undetermined species affiliation were i ... | 2011 | 21812718 |
| complete genome sequence of brachyspira intermedia reveals unique genomic features in brachyspira species and phage-mediated horizontal gene transfer. | abstract: | 2011 | 21816042 |
| phenotypic and genetic diversity among intestinal spirochaetes (genus brachyspira) in free-living wild mallards (anas platyrhynchos) sampled in southern sweden. | brachyspira spp. are anaerobic intestinal spirochaetes that colonize vertebrates. some species cause enteric diseases in pigs, chickens and possibly in humans, whereas others display a commensual relationship with their hosts. the aims were to investigate the prevalence among colonized free-living wild mallards (anas platyrhynchos) of three enteropathogenic brachyspira spp., and to describe the biodiversity of brachyspira spp. isolates. isolates from 150 birds were screened by pcr for 3 pathogen ... | 2011 | 22036527 |
| Demonstration of genes encoding virulence and virulence life-style factors in Brachyspira spp. isolates from pigs. | The distribution of many genes encoding virulence and virulence life-style (VL-S) factors in Brachyspira (B.) hyodysenteriae and other Brachyspira species are largely unknown. Their knowledge is essential e.g. for the improvement of diagnostic methods targeting the detection and differentiation of the species. Thus 121 German Brachyspira field isolates from diarrhoeic pigs were characterized down to the species level by restriction fragment length polymorphism analysis of the nox gene and subseq ... | 2011 | 22047713 |
| comparison of atypical brachyspira spp. clinical isolates and classic strains in a mouse model of swine dysentery. | multiple brachyspira spp. can colonize the porcine colon, and the presence of the strongly beta-hemolytic brachyspira hyodysenteriae is typically associated with clinical swine dysentery. recently, several brachyspira spp. have been isolated from the feces of pigs with clinical disease suggestive of swine dysentery, yet these isolates were not identified as b. hyodysenteriae by genotypic or phenotypic methods. this study used a mouse model of swine dysentery to compare the pathogenic potential o ... | 2012 | 22770518 |
| comparative virulence of clinical brachyspira spp. isolates in inoculated pigs. | classical swine dysentery is associated with the presence of the strongly beta-hemolytic brachyspira hyodysenteriae. however, multiple brachyspira spp. can colonize the porcine colon. since 2008, several brachyspira spp. not identified as b. hyodysenteriae by genotypic and/or phenotypic methods have been isolated from the feces of pigs with clinical disease typical of swine dysentery. in the current study, 8 clinical isolates, including 5 strongly beta-hemolytic and 3 weakly beta-hemolytic brach ... | 2012 | 22956484 |
| occurrence of viable brachyspira spp. on carcasses of spent laying hens from supermarkets. | brachyspira spp. are frequent inhabitants of the chicken's intestine and some have been associated with enteric disease in humans. we studied contamination with brachyspira spp. of carcasses of spent laying hens as a possible source of infections for humans and animals that may eat this meat. eleven batches of hen carcasses, for a total of 110 carcasses, were bought in belgian supermarkets during 2009-2010. carcass rinse samples were examined for the presence of brachyspira. brachyspira spp. wer ... | 2012 | 22986196 |
| occurrence of pathogens in wild rodents caught on swedish pig and chicken farms. | a total of 207 wild rodents were caught on nine pig farms, five chicken farms and five non-farm locations in sweden and surveyed for a selection of bacteria, parasites and viruses. lawsonia intracellularia and pathogenic yersinia enterocolitica were only detected in rodents on pig farms (9% and 8% prevalence, respectively) which indicate that these agents are more likely to be transmitted to rodents from pigs or the environment on infected farms. brachyspira hyodysenteriae (1%), brachyspira inte ... | 2013 | 23174339 |
| in vitro sensitivity of poultry brachyspira intermedia isolates to essential oil components and in vivo reduction of brachyspira intermedia in rearing pullets with cinnamaldehyde feed supplementation. | cecal enteritis due to brachyspira infections tends to be chronic in laying hens. limited availability of antimicrobial drugs for use in laying hens emphasizes the need for alternative control measures. a broth microdilution method was used to determine the antimicrobial susceptibility of 20 brachyspira intermedia field isolates from laying hen flocks to components of essential oils (eo). minimal inhibitory concentration (mic) distributions, obtained for 8 eo components, were all monomodal. cinn ... | 2013 | 23571329 |
| fluorescent in situ hybridization for detection of "brachyspira hampsonii" in porcine colonic tissues. | swine dysentery is classically associated with infection by the strongly beta-hemolytic brachyspira hyodysenteriae; however, the proposed novel species "brachyspira hampsonii" has also been isolated from clinical cases of dysentery in the united states and canada. microbial culture is highly sensitive for detecting brachyspira in clinical samples but requires several days for completion and is often followed by molecular testing for speciation. alternatively, in situ hybridization using molecula ... | 2013 | 23572452 |
| identification of weakly haemolytic brachyspira isolates recovered from pigs with diarrhoea in spain and portugal and comparison with results from other countries. | weakly haemolytic anaerobic intestinal spirochaetes of the genus brachyspira are commonly identified based on species-specific gene sequences. apart from the pathogenic brachyspira pilosicoli, the distribution and disease associations of the other weakly haemolytic brachyspira species in pigs have not been comprehensively investigated. in this study weakly haemolytic brachyspira isolates (n=67) from spanish and portuguese pigs with diarrhoea, negative in a routine diagnostic pcr for b. pilosicol ... | 2013 | 23928181 |
| the pathogenic intestinal spirochaete brachyspira pilosicoli forms a diverse recombinant species demonstrating some local clustering of related strains and potential for zoonotic spread. | brachyspira pilosicoli is an anaerobic spirochaete that can colonizes the large intestine of many host species. infection is particularly problematic in pigs and adult poultry, causing colitis and diarrhea, but it is also known to result in clinical problems in human beings. despite the economic importance of the spirochaete as an animal pathogen, and its potential as a zoonotic agent, it has not received extensive study. | 2013 | 23957888 |
| "brachyspira hampsonii" clade i isolated from belgian pigs imported to germany. | this report describes the detection of "brachyspira (b.) hampsonii" clade i in belgian pigs imported to germany. two of seventeen pigs from one herd were reported positive for brachyspira hyodysenteriae by culture in a belgian diagnostic laboratory, but negative for this brachyspira species by specific pcr. in this study, from 22 fecal samples and 2 colon contents of these animals various brachyspira species were cultured and identified by nox-rflp as brachyspira murdochii, brachyspira innocens ... | 2014 | 24332829 |
| matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid identification of brachyspira species isolated from swine, including the newly described "brachyspira hampsonii". | the brachyspira species traditionally associated with swine dysentery and other diarrheal diseases in pigs are brachyspira hyodysenteriae, brachyspira pilosicoli, and, to a lesser extent, brachyspira murdochii. "brachyspira hampsonii" is a recently proposed novel species that causes clinical disease similar to that caused by b. hyodysenteriae. matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) systems are increasingly available in veterinary diagnostic la ... | 2014 | 25012082 |
| intestinal spirochaetes (brachyspira spp.) colonizing flocks of layer and breeder chickens in malaysia. | avian intestinal spirochaetosis causes problems including delayed onset of lay and wet litter in adult chickens, and results from colonization of the caecae/rectum with pathogenic intestinal spirochaetes (genus brachyspira). because avian intestinal spirochaetosis has not previously been studied in south east asia, this investigation was undertaken in malaysia. faecal samples were collected from 25 farms and a questionnaire was administered. brachyspira species were detected by polymerase chain ... | 2014 | 25246135 |
| investigation of the impact of increased dietary insoluble fiber through the feeding of distillers dried grains with solubles (ddgs) on the incidence and severity of brachyspira-associated colitis in pigs. | diet has been implicated as a major factor impacting clinical disease expression of swine dysentery and brachyspira hyodysenteriae colonization. however, the impact of diet on novel pathogenic strongly beta-hemolytic brachyspira spp. including "b. hampsonii" has yet to be investigated. in recent years, distillers dried grains with solubles (ddgs), a source of insoluble dietary fiber, has been increasingly included in diets of swine. a randomized complete block experiment was used to examine the ... | 2014 | 25485776 |
| experimental infection of laying hens with serpulina intermedia causes reduced egg production and increased faecal water content. | serpulina intermedia strain hb60, isolated from an australian hen with diarrhoea, was used to infect 10 individually caged 14-week-old laying hens. another 10 birds were sham inoculated with sterile broth. birds were kept for 16 weeks, and faecal water content, egg production and body weights recorded. strain hb60 was isolated from the faeces of nine of the infected birds at irregular intervals throughout the experiment, and from their caeca at slaughter. infected birds tended to be lighter and ... | 1999 | 26911496 |
| prevalence and disease association of intestinal spirochaetes in chickens in eastern australia. | faecal samples (n = 1786) from chickens in broiler breeder (n = 28), layer (n = 22) or broiler (n = 19) flocks in the eastern states of australia were cultured for intestinal spirochaetes. overall, birds in 42.9% of broiler breeder and 68.2% of layer flocks were colonized with spirochaetes, but no birds in broiler flocks were infected. colonization rates in infected flocks ranged from 10 to 100% of birds sampled. faeces from colonized flocks were on average 14% wetter than those from non-coloniz ... | 1999 | 26911598 |
| [detection of brachyspira pilosicoli and other brachyspira species in argentine poultry farms]. | some species of the genus brachyspira such as brachyspira pilosicoli, brachyspira intermedia and brachyspira alvinipulli are pathogenic species capable of producing disease in laying hens. in our country, the presence of b. pilosicoli and other species of brachyspira has been reported in pigs and dogs but there is no record of their presence in poultry. fecal and cecal content samples from 34 laying hens of 4, 12 and 24 months of age from two farms were analyzed by isolation, biochemical tests a ... | 2016 | 26952690 |