Publications

reclassification of thermomonospora and microtetraspora.	almost complete 16s rrna sequences from seven thermomonospora strains, thermomonospora curvata, thermomonospora formosensis, thermomonospora fusca, thermomonospora mesophila, thermomonospora chromogena, thermomonospora alba and thermomonospora mesouviformis (a synonym of thermomonospora alba) were determined and subjected to phylogenetic analysis together with the sequences from all the representative members of the suborder streptosporangineae. on the basis of phylogenetic, chemotaxonomic and p ...	1998	9731279
taxonomic re-evaluation of 'nocardiopsis' sp. k-252t (= nrrl 15532t): a proposal to transfer this strain to the genus nonomuraea as nonomuraea longicatena sp. nov.	re-evaluation of the taxonomic position of strain k-252t, which produces the compound k-252a, showed that the strain does not belong to the genus nocardiopsis suggested previously. strain k-252t formed aerial mycelia with long spore chains, and hyphal fragmentation was not observed. the cell wall chemotype of the strain was iii/b, the major menaquinone was mk-9 (iii, viii-h4), the phospholipid pattern was piv and the major cellular fatty acids were 10me-c17:0, iso-c16:0 and c16:0. phylogenetic a ...	1999	10555344
semisynthetic derivatives of madurahydroxylactone and their antibacterial activities.	madurahydroxylactone is a secondary metabolite from nonomuria rubra (former actinomadura rubra) with in vitro activity against gram-positive bacteria and belongs to the family of benzo[a]naphthacenequinones. a series of derivatives of madurahydroxylactone were synthesized to investigate the effect on the antibacterial activity. reaction with alcohols and amines gave cyclic acetals or aminals derived from the lactone form, whereas other amino reagents like hydroxylamines and acyl or sulfonyl hydr ...	1999	10656576
kyanomycin, a complex of unusual anthracycline-phospholipid hybrids from nonomuria species.	a blue secondary metabolite complex, named kyanomycin (2), in addition to epsilon-rhodomycinone (1), was detected in the mycelium extract of nonomuria sp. nn 22303 by hplc-diode array and hplc-electrospray mass spectrometry screening. the chemical structures of the novel compounds were determined to be unusual anthracycline-phosphatidylethanolamine hybrids by spectroscopic and chemical degradation experiments.	2000	10757705
proposal to transfer actinomadura carminata to a new subspecies of the genus nonomuraea as nonomuraea roseoviolacea subsp. carminata comb. nov.	an anthracycline-producing actinomycete (strain sf2303) was compared with two other anthracycline producers, actinomadura carminata ifo 15903t and nonomuraea roseoviolacea ifo 14098t, using morphological, physiological, chemotaxonomic and molecular-genetic criteria. the morphological and cultural characteristics of these three strains are similar. the physiological properties of strain sf2303 and n. roseoviolacea ifo 14098t are very similar, but are different from those of a. carminata ifo 15903 ...	2001	11411710
nonomuraea dietzii sp. nov..	the taxon 'sebekia benihana', an actinomycete of industrial significance, has been subjected to a polyphasic taxonomic study. 16s rdna sequence analyses of strain dsm 44320 (= nrrl 11111) revealed 100% similarity to the 16s rdna sequence of nonomuraea roseola dsm 43767t and high values (>98.5%) to some other species of this genus. dna-dna similarity values of less than 31% observed between strain dsm 44320 and n. roseola dsm 43767t, nonomuraea recticatena dsm 43937t and nonomuraea africana dsm 4 ...	2001	11491343
[isolation of actinomycetes from soil using extremely high frequency radiation].	a new method employing extremely high frequencies (ehfs) is proposed for the selective isolation of actinomycetes from soil. the pretreatment of soil suspensions with ehf wavelengths of 5.6 and 7.1 mm led to a nonselective isolation of actinomycetes. at the same time, the irradiation of soil suspensions within wavelength bands of 3.8-5.8 and 8-11.5 mm considerably augmented the total number of isolated actinomycetes and increased the fraction of the isolated rare genera by 2 and 7 times, respect ...	2002	11910800
a novel type of nonsteroidal estrone sulfatase inhibitors.	madurahydroxylactone (mhl) is a secondary metabolite produced by the soil bacterium nonomuria rubra and belongs to the family of benzo[a]naphthacenequinones. we report the initial results and structure-activity relationships of our study into a series of thiosemicarbazone derivatives of madurahydroxylactone as potential nonsteroidal inhibitors of the enzyme estrone sulfatase. the most active compound, the cyclohexylthiosemicarbazone, was shown to be a non-competitive inhibitor with a k(i) of 0.3 ...	2002	11992772
biosynthesis of the cyclitol moiety of pyralomicin 1a in nonomuraea spiralis mi178-34f18.	the biosynthetic pathway leading to the cyclitol moiety of pyralomicin 1a (1) in nonomuraea spiralis mi178-34f18 has been studied using a series of 2h-labeled potential precursors. the results demonstrate that 2-epi-5-epi-valiolone (7), a common precursor for acarbose (4) and validamycin a (5) biosynthesis, is an immediate precursor of pyralomicin 1a. 5-epi-valiolone (8) was also incorporated into 1, albeit less efficiently than 7. other potential intermediates, such as valiolone (9), valienone ...	2002	12195964
three-dimensional structures of thermophilic beta-1,4-xylanases from chaetomium thermophilum and nonomuraea flexuosa. comparison of twelve xylanases in relation to their thermal stability.	the crystal structures of thermophilic xylanases from chaetomium thermophilum and nonomuraea flexuosa were determined at 1.75 and 2.1 a resolution, respectively. both enzymes have the overall fold typical to family 11 xylanases with two highly twisted beta-sheets forming a large cleft. the comparison of 12 crystal structures of family 11 xylanases from both mesophilic and thermophilic organisms showed that the structures of different xylanases are very similar. the sequence identity differences ...	2003	12653995
production of the glycopeptide antibiotic a40926 by nonomuraea sp. atcc 39727: influence of medium composition in batch fermentation.	nonomuraea sp. atcc 39727 is a novel actinomycete species and the producer of a40926, a glycopeptide antibiotic structurally similar to teichoplanin. in the present study, a defined minimal medium was designed for nonomuraea fermentation. the influence of initial phosphate, glucose and ammonium concentrations on antibiotic productivity was investigated in batch fermentation and the effect of glucose limitation was studied in fed-batch fermentation. it was found that low initial concentrations of ...	2003	12687487
[the application of succession analysis in combination with extremely high-frequency irradiation to the selective isolation of actinomycetes from soil].	a new method employing succession analysis and extremely high-frequency (ehf) irradiation is proposed for the selective isolation of actinomycetes from soil. total actinomycetes were efficiently isolated from soil suspensions irradiated in the wavelength band 4.6-5.8 mm on the 14th and 45th days of succession initiated by soil wetting and from soil suspensions irradiated in the wavelength band 8-11.5 mm on the 7th day of succession. the rare actinomycete genera actinomadura, micromonospora, nono ...	2003	12698804
production of demannosyl-a40926 by a nonomuraea sp. atcc 39727 mutant strain.		2003	12760688
the gene cluster for the biosynthesis of the glycopeptide antibiotic a40926 by nonomuraea species.	the glycopeptide a40926 is the precursor of dalbavancin, a second-generation glycopeptide currently under clinical development. the dbv gene cluster, devoted to a40926 biosynthesis, was isolated and characterized from the actinomycete nonomuraea species atcc39727. from sequence analysis, 37 open reading frames (orfs) participate in a40926 biosynthesis, regulation, resistance, and export. of these, 27 orfs find a match in at least one of the previously characterized glycopeptide gene clusters, wh ...	2003	12837387
a gene transfer system for the glycopeptide producer nonomuraea sp. atcc39727.	the filamentous actinomycete nonomuraea sp. atcc39727 produces the industrially important glycopeptide antibiotic a40926. we developed a gene transfer system based on intergeneric conjugation from escherichia coli. analysis of the ex-conjugants revealed that the incoming plasmid pset152 had integrated at two sites in the nonomuraea genome. one of these was characterized and found to be highly related to other phic31 attb sites described in streptomyces spp., including the core tts sequence, wher ...	2003	12900021
nonomuraea terrinata sp. nov., a novel soil actinomycete.	the taxonomic position of a soil isolate, strain e626, was evaluated using the polyphasic approach. the organism was found to have chemical and morphological features consistent with its assignment to the genus nonomuraea, a member of the family streptosporangiaceae. strain e626 consistently formed a distinct phyletic line within the streptosporangiaceae 16s rdna tree using four different algorithms. furthermore, the taxonomic distinctness of the organism is underpinned by a range of phenotypic ...	2003	12906356
production and characterization of monochlorinated and dechlorinated a40926 derivatives.	nonomuraea sp. atcc 39727 is the producer of the a40926 complex of lipoglycopeptide antibiotics which contain chlorine atoms in amino acids 3 and 6 of the peptide backbone. using a classical mutagenesis and selection approach we have isolated a nonomuraea sp. atcc 39727 mutant strain able to direct production towards new a40926 analogues dechloro-a40926 (ddc) lacking two chlorine atoms and the two monochloro-a40926 (mdc1 and mdc2) that are not produced fermenting the wild type strain. dechlorina ...	2003	14632287
characterization of plasma membrane respiratory chain and atpase in the actinomycete nonomuraea sp. atcc 39727.	we have characterized the respiratory system of the aerobic actinomycete nonomuraea sp. atcc 39727. the plasma membrane of the microorganism is shown to contain a protonmotive respiratory chain and h+-atpase. the respiratory chain is made up of a rotenone-sensitive nadh-quinone oxidoreductase, a four subunits aa3-type cytochrome c oxidase and a bc1 complex. the h+-atpase is characterized as an f0f1-type on the basis of its sensitivity to specific inhibitors; the enzyme is also inhibited by mm co ...	2003	14638429
high-yield production of a bacterial xylanase in the filamentous fungus trichoderma reesei requires a carrier polypeptide with an intact domain structure.	a bacterial xylanase gene, nonomuraea flexuosa xyn11a, was expressed in the filamentous fungus trichoderma reesei from the strong cellobiohydrolase 1 promoter as fusions to a variety of carrier polypeptides. by using single-copy isogenic transformants, it was shown that production of this xylanase was clearly increased (up to 820 mg/liter) when it was produced as a fusion protein with a carrier polypeptide having an intact domain structure compared to the production (150 to 300 mg/liter) of fusi ...	2003	14660351
valine influences production and complex composition of glycopeptide antibiotic a40926 in fermentations of nonomuraea sp. atcc 39727.	in actinomycetes the catabolism products of branched chain amino acids provide biosynthetic precursors for the formation of several lipid-containing antibiotics. we have determined in nonomuraea sp. atcc 39727 the effect of valine on production of glycopeptide antibiotic a40926, which is a complex of factors structurally differing in fatty acid moieties. addition of valine to minimal medium increased a40926 production and modified complex composition towards a mono-component. similar results wer ...	2004	15032484
reclassification as a nonomuraea sp. of the strain atcc 39727, producing the glycopeptide antibiotic a40926.		2004	15032489
identification of the entner-doudoroff pathway in an antibiotic-producing actinomycete species.	the metabolic network of the central carbon metabolism represents the backbone of cellular metabolism and provides the precursors and cofactors required for synthesis of secondary metabolites. it is therefore pivotal to map the operating metabolic network in the central carbon metabolism in order to design metabolic engineering strategies towards construction of more efficient producers of specific metabolites. in this context, methods that allow rapid and reliable mapping of the central carbon ...	2004	15101992
glucose metabolism in the antibiotic producing actinomycete nonomuraea sp. atcc 39727.	the actinomycete nonomuraea sp. atcc 39727, producer of the glycopeptide a40926 that is used as precursor for the novel antibiotic dalbavancin, has an unusual carbon metabolism. glucose is primarily metabolized via the entner-doudoroff (ed) pathway, although the energetically more favorable embden-meyerhof-parnas (emp) pathway is present in this organism. moreover, nonomuraea utilizes a ppi-dependent phosphofructokinase, an enzyme that has been connected with anaerobic metabolism in eukaryotes a ...	2004	15472928
thermostable xylanases, xyn10a and xyn11a, from the actinomycete nonomuraea flexuosa: isolation of the genes and characterization of recombinant xyn11a polypeptides produced in trichoderma reesei.	two endoxylanases, nf xyn11a and nf xyn10a, were cloned from a nonomuraea flexuosa (previously actinomadura flexuosa) dsm43186 genomic expression library in escherichia coli. the coding sequences of xyn11a and xyn10a consist of 344 and 492 amino acids, respectively. the catalytic domains belong to family 11 and family 10 of glycoside hydrolases. the c-termini share strong amino acid sequence similarity to carbohydrate-binding module (cbm) families cbm2 and cbm13, respectively. native nf xyn11a, ...	2005	15650852
nonomuraea kuesteri sp. nov.	a gram-positive, aerobic, non-motile actinomycete, strain gw 14-1925(t), that formed branched substrate and aerial mycelium was studied using a polyphasic approach. on the basis of 16s rrna gene sequence similarity studies, strain gw 14-1925(t) was shown to belong to the genus nonomuraea, being most closely related to nonomuraea longicatena (97.9 %), nonomuraea turkmeniaca (98.9 %), nonomuraea helvata (98.6 %), nonomuraea polychroma (98.5 %), nonomuraea salmonaea (98.3 %), nonomuraea roseoviolac ...	2005	15774673
expression in streptomyces lividans of nonomuraea genes cloned in an artificial chromosome.	a bacterial artificial chromosomal library of nonomuraea sp. atcc39727 was constructed using escherichia coli-streptomyces artificial chromosome (esac) and screened for the presence of dbv genes known to be involved in the biosynthesis of the glycopeptide a40926. dbv genes were cloned as two large, partially overlapping, fragments and transferred into the host streptomyces lividans, thus generating strains s. lividanscolon, two colonsnmesac50 and s. lividanscolon, two colonsnmesac57. the heterol ...	2005	15821915
reclassification of nonomuraea flexuosa (meyer 1989) zhang et al. 1998 as thermopolyspora flexuosa gen. nov., comb. nov., nom. rev.	a polyphasic study was undertaken to clarify the taxonomic position of nonomuraea flexuosa dsm 41386(t). the distinct 16s rrna gene sequence phyletic branch formed by this strain was equated with nine related monophyletic clades composed of representatives of the genera classified in the family streptosporangiaceae. the organism produced a pcr product characteristic of this taxon when examined using a set of oligonucleotide primers specific for members of the family streptosporangiaceae. strain ...	2005	16166698
[study on biodiversity of type i & ii polyketide synthesis genes positive microorganisms].	some soil samples were collected from different places in yunnan provinces, china. 876 bacteria or actinomycete strains were isolated using glucose-peptone-yeast extract agar, starch-casein agar and glycerol-asparagine agar with these soil samples, of which about 100 strains belong to genus streptomyces and the others belong to rare actinomycetes or bacteria. with polyketide synthesis gene screening, 75 strains were picked up as type i & ii polyketide synthesis gene positive strains. then 10 str ...	2005	16496684
a derivative of the glycopeptide a40926 produced by inactivation of the beta-hydroxylase gene in nonomuraea sp. atcc39727.	the actinomycete nonomuraea sp. atcc39727 produces the glycopeptide a40926. in the corresponding dbv cluster, orf28 encodes a putative hydroxylase. a gene replacement mutant of orf28 in nonomuraea produces a small amount of an a40926-related metabolite, 16 amu smaller than the parent compound, which was identified as the desoxyderivative of a40926 lacking the beta-hydroxyl group on the tyrosine moiety. this result demonstrates that orf28 is actually involved in the formation of the beta-hydroxyt ...	2006	16499611
structure and expression of the atp operon coding for f1f0-atp synthase from the antibiotic-producing actinomycete nonomuraea sp. atcc 39727.	nonomuraea sp. atcc 39727 is a poorly characterized actinomycete, producer of the glycopeptide antibiotic a40926. in this study, the nucleotide sequence of the atp operon coding for f1f0-atp synthase of nonomuraea sp. atcc 39727 was determined. it consisted of ten open reading frames arranged in the order atpi (encoding the i protein), orfx, atpb (a subunit), atpe (c subunit), atpf (b subunit), atph (delta subunit), atpa (alpha subunit), atpg (gamma subunit), atpd (beta subunit) and atpc (epsilo ...	2006	16545948
understanding and manipulating glycopeptide pathways: the example of the dalbavancin precursor a40926.	glycopeptide antibiotics represent an important class of microbial compounds produced by several genera of actinomycetes. the emergence of resistance to glycopeptides among enterococci and staphylococci has prompted the search for second-generation drugs of this class and semi-synthetic derivatives are currently under clinical trials. dalbavancin is obtained by chemical modification of the natural glycopeptide a40926, produced by a nonomuraea sp. recently, there has been considerable progress in ...	2006	16761167
hydroxylation of oleanolic acid to queretaroic acid by cytochrome p450 from nonomuraea recticatena.	a gene for cytochrome p450 (moxa) from nonomuraea recticatena, coexpressed with camab for pseudomonad redox partners in escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. when we used the p450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the e. coli cell. in an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.	2006	16960364
absolute configuration and antitumor activity of myxochelin a produced by nonomuraea pusilla tp-a0861.	in the screening of antitumor compounds from microbial secondary metabolites, myxochelin a was isolated from a culture broth of nonomuraea pusilla tp-a0861. the absolute configuration was determined to be s by synthesizing both enantiomers from an l- or d-lysine derivative and comparing their specific rotations. both enantiomers of myxochelin a showed remarkable inhibitory effects on the invasion of murine colon 26-l5 carcinoma cells at non-cytotoxic concentrations.	2006	17256468
nonomuraea aegyptia sp. nov., a novel actinomycete isolated from a sand dune.	the taxonomic position of an unknown actinomycete isolated from a sand dune soil sample collected at borg el-arab in egypt was established using a combination of genotypic and phenotypic data. isolate s136(t) had chemotaxonomic and morphological properties consistent with its classification in the genus nonomuraea and formed a distinct phyletic line in the nonomuraea 16s rrna gene tree. it was most closely related to the type strains of nonomuraea helvata, nonomuraea kuesteri and nonomuraea turk ...	2007	17318331
isolation and partial characterization of antimicrobial compounds from a new strain nonomuraea sp. nm94.	an actinomycete strain nm94 was isolated from a saharan soil sample by a dilution agar plating method using chitin-vitamins b medium supplemented with penicillin. the strain presented the morphological and chemical characteristics of the genus nonomuraea. on the basis of 16s rdna analysis and physiological tests, this isolate was found to be quite different from the known species of nonomuraea and might be new. the strain nm94 secreted several antibiotics on yeast extract malt extract glucose me ...	2007	17318487
increased production of xylanase by expression of a truncated version of the xyn11a gene from nonomuraea flexuosa in trichoderma reesei.	we have previously shown that the nonomuraea flexuosa xyn11a polypeptides devoid of the carbohydrate binding module (cbm) have better thermostability than the full-length xylanase and are effective in bleaching of pulp. to produce an enzyme preparation useful for industrial applications requiring high temperature, the region encoding the cbm was deleted from the n. flexuosa xyn11a gene and the truncated gene was expressed in trichoderma reesei. the xylanase sequence was fused to the t. reesei ma ...	2007	17384308
genetic organization of the biosynthetic gene cluster for the indolocarbazole k-252a in nonomuraea longicatena jcm 11136.	indolocarbazole metabolite k-252a is a natural product that was previously reported as a potent protein kinase c inhibitor with in vitro and in vivo potency. from a biosynthetic viewpoint, this compound possesses structurally interesting features such as an unusual furanosyl sugar moiety, which are absent in the well-studied staurosporine and rebeccamycin. a cosmid library from genomic dna of nonomuraea longicatena jcm 11136 was constructed and screened for the presence of genes to be involved i ...	2007	17396254
biotransformations of lipoglycopeptides to obtain novel antibiotics.	the emergence of resistance among gram-positive pathogens towards glycopeptide antibiotics has stimulated the research of second-generation molecules with improved activity and expanded antimicrobial spectrum. in this paper we investigate biotransformations as a way to generate novel teicoplanin- and a40926-like molecules. a range of commercial enzymes, fungi and actinomycetes were tested on a40926 and on its semi-synthetic derivatives (mdl 63,246 and dalbavancin). oxidation of dalbavancin to md ...	2007	17456978
a two-dimensional electrophoresis and mass spectrometry protein analysis of the antibiotic producer nonomuraea sp. atcc 39727 in different growth conditions.	nonomuraea sp. atcc 39727 is an aerobic actinomycete, industrially important as a producer of the glycopeptide a40926, which is used as a precursor of the semi-synthetic antibiotic dalbavancin. previous studies showed that the production of a40926 is depressed by calcium, but promoted by l-glutamine or l-asparagine. in this study, the protein expression changes of nonomuraea sp. atcc 39727 in these two different growth and antibiotic-production conditions have been investigated by two-dimensiona ...	2007	17573934
nonomuraea bangladeshensis sp. nov. and nonomuraea coxensis sp. nov.	two novel bacterial strains were isolated from sandy soil from cox's bazar, bangladesh. strains 5-10-10(t) and 5-38-42(t) were gram-positive, aerobic, non-motile actinomycetes that form branched substrate and aerial mycelium. on the basis of 16s rrna gene sequence similarity studies, the novel strains were shown to belong to the genus nonomuraea, being most closely related to nonomuraea fastidiosa. chemotaxonomic data supported the assignment of the novel strains as members of the genus nonomura ...	2007	17625184
nonomuraea candida sp. nov., a new species from south african soil.	a novel actinomycete, strain hmc10(t), was isolated from a soil sample taken from the banks of the gamka river in the swartberg nature reserve, western cape province, south africa. strain hmc10(t) was identified as a member of the genus nonomuraea by a polyphasic approach. strain hmc10(t) could be differentiated from other members of the genus nonomuraea on the basis of physiology and 16s rrna gene sequence analysis. dna-dna hybridization further differentiated strain hmc10(t) from its nearest p ...	2008	17636376
anthelmintic macrolactams from nonomuraea turkmeniaca ma7364.	two new macrolactams, 6-desmethyl-n-methylfluvirucin a1 (1) and n-methylfluvirucin a1 (2), have been isolated from the acetone extract of nonomuraea turkmeniaca ma7364. these compounds were isolated by bioassay-guided fractionation as part of our search for new anthelmintics. the structures of these compounds were elucidated by comparison of their nmr and ms data to those of previously reported fluvirucins and confirmed by 2d nmr. compound 1 exhibited in vitro activity (ec(90) 15 +/- 5 microg/ml ...	2007	17636954
crystal structure of cytochrome p450 moxa from nonomuraea recticatena (cyp105).	cytochrome p450 moxa (p450moxa) from a rare actinomycete nonomuraea recticatena belongs to the cyp105 family and exhibits remarkably broad substrate specificity. here, we demonstrate that p450moxa acts on several luciferin derivatives, which were originally identified as substrates of the human microsomal p450s. we also describe the crystal structure of p450moxa in substrate-free form. structural comparison with various bacterial and human microsomal p450s reveals that the p450moxa structure is ...	2007	17679139
nonomuraea maheshkhaliensis sp. nov., a novel actinomycete isolated from mangrove rhizosphere mud.	a strain of nonomuraea was isolated from maheshkhali, cox's bazar, an unexplored region of bangladesh. strain 16-5-14(t) is a gram-positive, aerobic, non-motile actinomycete that formed branched substrate and aerial mycelia. on the basis of 16s rrna gene sequence similarity studies, strain 16-5-14(t) was shown to belong to the genus nonomuraea, being most closely related to nonomuraea kuesteri. chemotaxonomic data supported allocation of the strain as a member of the genus nonomuraea. the strain ...	2007	17726296
phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor a40926.	the actinomycete nonomuraea sp. strain atcc 39727 produces the glycopeptide a40926, the precursor of the novel antibiotic dalbavancin. previous studies have shown that phosphate limitation results in enhanced a40926 production. the a40926 biosynthetic gene (dbv) cluster, which consists of 37 genes, encodes two putative regulators, dbv3 and dbv4, as well as the response regulator (dbv6) and the sensor-kinase (dbv22) of a putative two-component system. reverse transcription-pcr (rt-pcr) and real-t ...	2007	17873036
rare actinomycete bacteria from the shallow water sediments of the trondheim fjord, norway: isolation, diversity and biological activity.	actinomycete bacteria produce a wide variety of secondary metabolites with diverse biological activities, some of which have been developed for human medicine. rare actinomycetes are promising sources in search for new drugs, and their potential for producing biologically active molecules is poorly studied. in this work, we have investigated the diversity of actinomycetes in the shallow water sediments of the trondheim fjord (norway). due to the use of different selective isolation methods, an u ...	2007	17922759
anthelmintic macrolactams from nonomuraea turkmeniaca ma7381.	a new macrolactam, fluvirucin b0 (1), and two known macrolactams, sch 38516/fluvirucin b1 (2) and sch 39185/fluvirucin b3 (3), have been isolated from an acetone extract of a strain of nonomuraea turkmeniaca. these compounds were isolated by bioassay-guided fractionation as part of our search for new anthelmintics. the structures of these compounds were elucidated by comparison of their nmr and ms data to those of previously reported fluvirucins, and confirmed by 2d-nmr. 1approximately 3 exhibit ...	2008	18408323
factors influencing cell fatty acid composition and a40926 antibiotic complex production in nonomuraea sp. atcc 39727.	a40926 is a glycopeptide antibiotic complex consisting of several structurally related factors. it is produced by fermentation of nonomuraea sp. atcc 39727 and the complex components differ in the structure of the fatty acid moiety linked to the aminoglucuronic acid unit. in previous work, we observed that the production of single factors in glycopeptide antibiotic complexes could be selectively enhanced by the addition of suitable precursors to the culture medium. in this contribution, we exami ...	2008	18651189
[diversity of actinomycetes in wuling mountain].	to isolate new actinomycetes for discovering compounds of pharmaceutical importance.	2008	18837376
new cyclic tetrapeptides from nonomuraea sp. ta-0426 that inhibit glycine transporter type 1 (glyt1).	in the course of our search for natural antipsychotic agents, we isolated five new cyclic tetrapeptides from the fermentation broth of nonomuraea sp. ta-0426. these compounds turned out to be analogues of wss2220, which had been produced by the same actinomycete and showed strong inhibitory activity against glyt1. four of the present peptides exhibit more potent glyt1 inhibitory activities than wss2220.	2008	19013063
actinobacterial diversity from marine sediments collected in mexico.	seventeen different media known to support the growth and isolation of members of the class actinobacteria were evaluated as selective isolation media for the recovery of this microbial group from marine sediments samples collected in the gulf of california and the gulf of mexico. a general selective isolation procedure was employed for six sediments and nearly 300 actinomycetes were recovered from the selective isolation plates. full 16s rrna gene sequencing revealed that the isolates belonged ...	2009	19023674
activation of dormant bacterial genes by nonomuraea sp. strain atcc 39727 mutant-type rna polymerase.	there is accumulating evidence that the ability of actinomycetes to produce antibiotics and other bioactive secondary metabolites has been underestimated due to the presence of cryptic gene clusters. the activation of dormant genes is therefore one of the most important areas of experimental research for the discovery of drugs in these organisms. the recent observation that several actinomycetes possess two rna polymerase beta-chain genes (rpob) has opened up the possibility, explored in this st ...	2009	19047343
synthesis and evaluation of myxochelin analogues as antimetastatic agents.	myxochelin a (1) is an inhibitor of tumor cell invasion produced by the bacterium belonging to the genus nonomuraea. in order to obtain more potent inhibitors, a series of myxochelin analogues [2 and (s)-3-17] were synthesized through the coupling of lysine or diaminoalkane derivatives and appropriately protected hydroxybenzoate, followed by modification of functional groups and deprotection. these compounds were evaluated for their inhibitory activity against invasion of murine colon 26-l5 carc ...	2009	19282185
ribosomally synthesized thiopeptide antibiotics targeting elongation factor tu.	we identified the thiomuracins, a novel family of thiopeptides produced by a rare-actinomycete bacterium typed as a nonomuraea species, via a screen for inhibition of growth of the bacterial pathogen staphylococcus aureus. thiopeptides are a class of macrocyclic, highly modified peptides that are decorated by thiazoles and defined by a central six-membered heterocyclic ring system. mining the genomes of thiopeptide-producing strains revealed the elusive biosynthetic route for this class of antib ...	2009	19338336
brartemicin, an inhibitor of tumor cell invasion from the actinomycete nonomuraea sp.	brartemicin (1), a new trehalose-derived metabolite, was isolated from the culture broth of the actinomycete of the genus nonomuraea. its structure and absolute configuration were determined by spectroscopic analyses. the new compound inhibited the invasion of murine colon carcinoma 26-l5 cells with an ic(50) value of 0.39 microm in a concentration-dependent manner without showing cytotoxic effects.	2009	19358565
nonomuraea antimicrobica sp. nov., an endophytic actinomycete isolated from a leaf of maytenus austroyunnanensis.	a novel actinomycete strain, designated yim 61105(t), was isolated from a leaf of maytenus austroyunnanensis from the tropical rainforest in xishuangbanna, yunnan province, south-west china. a 16s rrna gene sequence analysis revealed that the organism belonged to the phylogenetic cluster of the genus nonomuraea and was most closely related to nonomuraea candida hmc10(t) (98.2 %), 'nonomuraea aegyptia' s136 (97.9 %), nonomuraea kuesteri gw 14-1925(t) (97.5 %) and nonomuraea turkmeniaca dsm 43926( ...	2009	19625420
nonomuraea rosea sp. nov.	a gram-positively staining, aerobic, non-motile actinomycete, strain gw 12687(t), that formed rose-pigmented colonies and branched substrate and aerial mycelia was studied in detail for its taxonomic position. on the basis of 16s rrna gene sequence similarity studies, strain gw 12687(t) was grouped into the genus nonomuraea, being most closely related to nonomuraea dietziae (97.6 %), nonomuraea africana (97.1 %), and nonomuraea kuesteri (97.1 %). the 16s rrna gene sequence similarity to other sp ...	2010	19666797
[selective isolation and diversity of acidophilic filamentous actinomycetes from acidic soils].	to establish effective methods for selective isolation of acidophilic filamentous actinomycetes from acidic soils, and to investigate their genus and species diversity.	2009	19673405
directed evolution of the actinomycete cytochrome p450moxa (cyp105) for enhanced activity.	actinomycete cytochrome p450 from nonomuraea recticatena nbrc 14525 (p450moxa) catalyzes the hydroxylation of a broad range of substrates, including fatty acids, steroids, and various aromatic compounds. hence, the enzyme is potentially useful in medicinal applications, but the activity is insufficient for practical use. here we applied directed evolution to enhance the activity. a random mutagenesis library was screened using 7-ethoxycoumarin as a substrate to retrieve 17 variants showing >2-fo ...	2009	19734686
protoplast preparation and reversion to the normal filamentous growth in antibiotic-producing uncommon actinomycetes.	protoplast preparation, regeneration and fusion represent essential tools for those poorly studied biotechnologically valuable microorganisms inapplicable with the current molecular biology protocols. the protoplast production and regeneration method developed for planobispora rosea and using the combination of hen egg-white lysozyme (hewl) and streptomyces globisporus mutanolysin was applied to a set of antibiotic-producing filamentous actinomycetes belonging to the streptosporangiaceae, microm ...	2010	20057514
novel mechanism of glycopeptide resistance in the a40926 producer nonomuraea sp. atcc 39727.	in glycopeptide-resistant enterococci and staphylococci, high-level resistance is achieved by replacing the c-terminal d-alanyl-d-alanine of lipid ii with d-alanyl-d-lactate, thus reducing glycopeptide affinity for cell wall targets. reorganization of the cell wall in these organisms is directed by the vanhax gene cluster. similar self-resistance mechanisms have been reported for glycopeptide-producing actinomycetes. we investigated glycopeptide resistance in nonomuraea sp. atcc 39727, the produ ...	2010	20308385
improved production of a40926 by nonomuraea sp. through deletion of a pathway-specific acetyltransferase.	nonomuraea strain atcc 39727 produces the glycopeptide a40926, used for manufacturing dalbavancin, currently in advanced clinical trials. from the gene cluster involved in a40926 biosynthesis, a strain deleted in dbv23 was constructed. this mutant can produce only the glycopeptides lacking the o-linked acetyl residue at position 6 of the mannose moiety, while, under identical fermentation conditions, the wild-type strain produces mostly glycopeptides carrying an acetylated mannose. furthermore, ...	2010	20414653
nonomuraea endophytica sp. nov., an endophytic actinomycete isolated from artemisia annua l.	a gram-stain-positive, aerobic, non-motile actinomycete strain, designated yim 65601(t), was studied by using a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequence comparisons revealed that strain yim 65601(t) should be assigned to the genus nonomuraea, with highest 16s rrna gene sequence similarities to nonomuraea candida hmc10(t) (98.8 %), n. salmonea dsm 43678(t) (98.7 %), n. turkmeniaca dsm 43926(t) (98.5 %), n. roseola dsm 43767(t) (98.4 %), n. dietziae ifo 14309(t) ( ...	2011	20435751
guanosine 5'-diphosphate 3'-diphosphate (ppgpp) as a negative modulator of polynucleotide phosphorylase activity in a 'rare' actinomycete.	with the beginning of the idiophase the highly phosphorylated guanylic nucleotides guanosine 5'-diphosphate 3'-diphosphate (ppgpp) and guanosine 5'-triphosphate 3'-diphosphate (pppgpp), collectively referred to as (p)ppgpp, activate stress survival adaptation programmes and trigger secondary metabolism in actinomycetes. the major target of (p)ppgpp is the rna polymerase, where it binds altering the enzyme activity. in this study analysis of the polynucleotide phosphorylase (pnpase)-encoding gene ...	2010	20545843
the diversity and anti-microbial activity of endophytic actinomycetes isolated from medicinal plants in panxi plateau, china.	traditional chinese medicinal plants are sources of biologically active compounds, providing raw material for pharmaceutical, cosmetic and fragrance industries. the endophytes of medicinal plants participate in biochemical pathways and produce analogous or novel bioactive compounds. panxi plateau in south-west sichuan in china with its unique geographical and climatological characteristics is a habitat of a great variety of medicinal plants. in this study, 560 endophytic actinomycetes were isola ...	2011	20567975
microbisporicin gene cluster reveals unusual features of lantibiotic biosynthesis in actinomycetes.	lantibiotics are ribosomally synthesized, posttranslationally modified peptide antibiotics. the biosynthetic gene cluster for microbisporicin, a potent lantibiotic produced by the actinomycete microbispora corallina containing chlorinated tryptophan and dihydroxyproline residues, was identified by genome scanning and isolated from an m. corallina cosmid library. heterologous expression in nonomuraea sp. atcc 39727 confirmed that all of the genes required for microbisporicin biosynthesis were pre ...	2010	20628010
nonomuraea wenchangensis sp. nov., isolated from mangrove rhizosphere soil.	strain 210417(t), which forms highly branched substrate and aerial mycelia, is a gram-positive, aerobic and non-motile actinomycete isolated from mangrove rhizosphere soil. 16s rrna gene sequence analysis showed that the strain should be classified in the genus nonomuraea, being most closely related to the type strains of nonomuraea coxensis (99.6 %) and nonomuraea bangladeshensis (99.3 %). chemotaxonomic properties [madurose as the major sugar in the cell wall; meso-diaminopimelic acid and n-ac ...	2010	20639224
methods for the genetic manipulation of nonomuraea sp. atcc 39727.	nonomuraea sp. atcc 39727 belongs to the streptosporangiaceae family of filamentous actinomycetes. this microorganism produces the teicoplanin-like glycopeptide a40926, which is the starting material for the synthesis of the second-generation glycopeptide dalbavancin. notwithstanding the strain's pharmaceutical relevance, the lack or poor efficiency of genetic tools to manipulate nonomuraea sp. atcc 39727 has hampered strain and product improvement. here we report the development of gene transfe ...	2010	20717702
nonomuraea rhizophila sp. nov., a novel actinomycete isolated from a rhizosphere soil.	a novel actinomycete, strain yim 67092t, was isolated from the rhizosphere soil of tripterygium wilfordii hook. f. collected from yunnan province, south-west china. the strain formed well differentiated aerial and substrate mycelia and grew in the presence of up to 7% (w/v) nacl. phylogenetic analysis based on the 16s rrna gene showed that strain yim 67092t belongs to the genus nonomuraea, with highest sequence similarity to nonomuraea rosea gw 12687t (99.0%). sequence similarities between strai ...	2010	20889768
nonomuraea maritima sp. nov., isolated from coastal sediment in bohai bay, china.	a novel actinomycete, strain fxj7.203t, was isolated from a coastal sediment sample collected in bohai bay, china. in 16s rrna gene sequence analyses, strain fxj7.203t always formed a unique monophyletic line within the genus nonomuraea, and was most closely related to nonomuraea turkmeniaca (97.9 %), nonomuraea candida (97.8 %), nonomuraea helvata (97.7 %) and nonomuraea rubra (97.5 %). morphological and chemotaxonomic characteristics supported the allocation of the strain to the genus nonomura ...	2010	21186288
synthesis and evaluation of trehalose-based compounds as anti-invasive agents.	brartemicin is a trehalose-based inhibitor of tumor cell invasion produced by the actinomycete of the genus nonomuraea. in order to explore the preliminary structure-activity relationship and obtain more potent inhibitors, a series of brartemicin analogs were synthesized through the mitsunobu coupling of the secondary hydroxyls benzyl protected a,a-d-trehalose with benzoic acid derivatives, followed by modification of functional groups and deprotection. these compounds were evaluated for their i ...	2011	21269828
computational design of an endo-1,4-beta-xylanase ligand binding site.	the field of computational protein design has experienced important recent success. however, the de novo computational design of high-affinity protein-ligand interfaces is still largely an open challenge. using the rosetta program, we attempted the in silico design of a high-affinity protein interface to a small peptide ligand. we chose the thermophilic endo-1,4-ß-xylanase from nonomuraea flexuosa as the protein scaffold on which to perform our designs. over the course of the study, 12 proteins ...	2011	21349882
actinomycete nonomuraea sp. isolated from indonesian soil is a new producer of inulin fructotransferase.	an actinomycete that excretes inulin fructotransferase to the culture supernatant was able to produce di-d-fructofuranose 1,2':2,3' dianhydride (dfa iii) from inulin, with the greatest rate of enzyme activity at 65-¦c and at a ph of 5.5. through chemotaxonomic and 16s rrna gene analysis, this strain was identified as genus nonomuraea in the streptosporangiaceae family. this is the first report of an inulin fructotransferase producer in this family.	2011	21396890
thermostable recombinant xylanases from nonomuraea flexuosa and thermoascus aurantiacus show distinct properties in the hydrolysis of xylans and pretreated wheat straw.	abstract: background: in the hydrolysis of lignocellulosic materials, thermostable enzymes decrease the amount of enzyme needed due to higher specific activity and elongate the hydrolysis time due to improved stability. for cost-efficient use of enzymes in large-scale industrial applications, high-level expression of enzymes in recombinant hosts is usually a prerequisite. the main aim of the present study was to compare the biochemical and hydrolytic properties of two thermostable recombinant gl ...	2011	21592333
comparison of the synergistic action of two thermostable xylanases from gh families 10 and 11 with thermostable cellulases in lignocellulose hydrolysis.	recombinant xylanase preparations from nonomuraea flexuosa (nf xyn, gh11) and thermoascus aurantiacus (ta xyn, gh10) were evaluated for their abilities to hydrolyze hydrothermally pretreated wheat straw. the gh family 10 enzyme ta xyn was clearly more efficient in solubilizing xylan from pretreated wheat straw. improvement of the hydrolysis of hydrothermally pretreated wheat straw by addition of the thermostable xylanase preparations to thermostable cellulases was evaluated. clear synergistic en ...	2011	21767947
nonomuraea jiangxiensis sp. nov., isolated from acidic soil collected in south-east china.	a novel actinomycete, designated strain fxj1.102t, was isolated from acidic soil collected in jiangxi province, south-east china. phylogenetic analysis based on 16s rrna gene sequences indicated that strain fxj1.102t belonged to the genus nonomuraea, and was most closely related to nonomuraea candida hmc 10t (98.8%), nonomuraea turkmeniaca dsm 43926t (98.3%), nonomuraea antimicrobica yim 61105t (97.9%) and 'nonomuraea aegyptia' s136 (97.5%). morphological characteristics of the strain were typic ...	2011	21828009
nonomuraea soli sp. nov., a novel actinomycete isolated from soil.	a straight-chain-spore actinobacterium strain, yim 120770(t), was isolated from soil. phylogenetic analysis on the basis of 16s rrna gene sequence comparison revealed that the isolate represents a distinct cluster within the clade of the genus nonomuraea and was most closely related to nonomuraea rhizophila yim 67092(t) (96.5% similarity). cells of strain yim 120770(t) grew in the presence of 0-3% (w/v) nacl, at 15-37°c and ph 7.0-8.0. the diagnostic amino acid was meso-diaminopimelic acid, cell ...	2011	21890732