Publications

some characteristics of anaerovibrio lipolytica a rumen lipolytic organism.	strains of anaerovibrio lipolytica isolated from sheep- and cow-rumen contents on a linseed oil -- rumen fluid -- agar medium fermented ribose, glycerol and dl-lactate. fermentation products from glycerol were propionate and succinate, while ribose, fructose and dl-lactate were fermented mainly to acetate, propionate and carbon dioxide. propionate is formed in this organism by the dicarboxylic acid pathway similarly as in propionibacteria. measurements of the rate of lipolysis by pure cultures s ...	1975	1080383
lipid-phase transitions of the strictly anaerobic bacteria veillonella parvula and anaerovibrio lipolytica.	as a basis for physicochemical studies on the membranes of the strictly anaerobic bacteria veillonella parvula, anaerovibrio lipolytica, and megasphaera elsdenii, the fatty acyl and alk-1-enyl moieties on the phosphoglycerides of these organism were characterized. uncommon is the high proportion of a heptadecenoic acyl and alk-1-enyl moiety in these three lactate-fermenting bacteria. in contrast to v. parvula and a. lipolytica, m. elsdenii contains high amounts of branched-chain acyl and alk-1-e ...	1975	1194242
uncommonly encountered, motile, anaerobic gram-negative bacilli associated with infection.	motile, anaerobic gram-negative bacilli belonging to the genera butyrivibrio, succinimonas, succinivibrio, anaerovibrio, wolinella, campylobacter, desulfovibrio, selenomonas, and anaerobiospirillum are being recognized in clinical specimens with increasing frequency. over a 12.5-year period at the va wadsworth medical center, 13 clinical specimens yielded one of these organisms. six isolates were recovered from infected wounds, five from respiratory tract specimens obtained from patients with an ...	1987	3321364
t-2 toxin metabolism by ruminal bacteria and its effect on their growth.	the effect of t-2 toxin on the growth rates of different bacteria was used as a measure of its toxicity. toxin levels of 10 micrograms/ml did not decrease the growth rate of selenomonas ruminantium and anaerovibrio lipolytica, whereas the growth rate of butyrivibrio fibrisolvens was uninhibited at toxin levels as high as 1 mg/ml. there was, however, a noticeable increase in the growth rate of b. fibrisolvens ce46 and ce51 and s. ruminantium in the presence of low concentrations (10 micrograms/ml ...	1987	3579272
antigenic distinctiveness of mobiluncus curtisii and mobiluncus mulieris.	a total of 26 mobiluncus strains (17 m. curtisii and 9 m. mulieris strains) were compared serologically by double immunodiffusion and immunoblotting against antisera prepared against representative isolates of each species. all strains from the same species were strongly reactive with homologous antisera but generally weakly reactive with antisera to the heterologous mobiluncus spp. the antisera did not react with strains of the unrelated genera campylobacter, succinivibrio, wolinella, actinomyc ...	1985	3924951
an electron microscopic study of anaerovibrio lipolytica (strain 5s) and its lipolytic enzyme.		1973	4124712
the presence and function of cytochromes in selenomonas ruminantium, anaerovibrio lipolytica and veillonella alcalescens.		1974	4362619
a study of the lipase produced by anaerovibrio lipolytica, a rumen bacterium.		1971	5104406
the lipases produced by anaerovibrio lipolytica in continuous culture.		1970	5500322
the influence of extracellular hydrogen on the metabolism of bacteroides ruminicola, anaerovibrio lipolytica and selenomonas ruminantium.	strains of three anaerobic rumen bacteria, bacteroides ruminicola, anaerovibrio lipolytica and selenomonas ruminantium, were able to use extracellular h2 to reduce fumarate to succinate. each bacterium possessed membrane-bound hydrogenase and fumarate reductase activity. membrane-bound cytochrome b was reducible by h2 and oxidizable by fumarate in each bacterium. the apparent km values for hydrogen of the hydrogenases were 4 . 5 x 10(-6) m, 1 . 4 x 10(-5) m and 4 . 4 x 10(-5) m for b. ruminicola ...	1980	6785381
effects of lasalocid or monensin on lactate-producing or -using rumen bacteria.	lasalocid or monensin inhibited most of the lactate-producing rumen bacteria (butyrivibrio fibrisolvens, eubacterium cellulosolvens, e. ruminantium, lachnospira multiparus, lactobacillus ruminis, l. vitulinus, ruminococcus albus, r. flavefaciens, streptococcus bovis). minimum inhibitory concentrations ranged from .38 to 3.0 micrograms/ml. among the lactate producers, those that produce succinate as a major end product (bacteroides, selenomonas, succinimonas, succinivibrio) were not inhibited by ...	1981	7275867
the in vitro uptake and metabolism of peptides and amino acids by five species of rumen bacteria.	streptococcus bovis jb1, prevotella ruminicola b(1)4, selenomonas ruminantium z108, fibrobacter succinogenes s85 and anaerovibrio lipolytica 5s were incubated with either 14c-peptides (mol. wt, 200-1000) or 14c-amino acids to compare their rates of uptake and metabolism. in experiment 1, the bacteria were grown and incubated in a complex medium, but no uptake of 14c-labelled substrates occurred. when casein digest was omitted, uptake rates of 14c-peptides were different (p < 0.01) with each spec ...	1995	7698948
gc-ms analysis of diaminopimelic acid stereoisomers and amino acid enantiomers in rumen bacteria.	the amounts and the configuration of the stereoisomers of 2,6-diaminopimelic acid (dap) and the enantiomeric content of other amino acids were determined in five individual species (fibrobacter succinogenes, streptococcus bovis, selenomonas ruminantium, prevotella ruminicola and anaerovibrio lipolytica) of rumen bacteria, and in samples of mixed rumen bacteria isolated from sheep. the separation and quantification of the dap stereoisomers was achieved by gas chromatography (gc) of trifluoroacety ...	1999	10191943
a re-evaluation of the taxonomy of the genus anaerovibrio, with the reclassification of anaerovibrio glycerini as anaerosinus glycerini gen. nov., comb. nov., and anaerovibrio burkinabensis as anaeroarcus burkinensis [corrig.] gen. nov., comb. nov.	chemotaxonomic, electron microscopic and 16s rrna gene sequence analyses of the three described species of the genus anaerovibrio demonstrated only remote similarities to each other. the 16s rrna gene sequence similarities between anaerovibrio lipolytica, anaerovibrio glycerini and anaerovibrio burkinabensis and the derived phylogenetic relationships of the three species studied fell below genus level. all three species clustered within the sporomusa-pectinatus-selenomonas phyletic group. each s ...	1999	10555370
covalent linkage of polyamines to peptidoglycan in anaerovibrio lipolytica.	spermidine and cadaverine were found to be constituents of the cell wall peptidoglycan of anaerovibrio lipolytica, a strictly anaerobic bacterium. the peptidoglycan was degraded with the n-acetylmuramyl-l-alanine amidase and endopeptidase into two peptide fragments, peptide i and peptide ii, at a molar ratio of 4:1. peptides i and ii were identified as l-alanine-d-glutamic acid(alphacadaverine)gammameso-diaminopimelic acid (dap)-d-alanine and l-alanine-d-glutamic acid(alphaspermidine)gammameso-d ...	2000	10648544
diet-dependent shifts in the bacterial population of the rumen revealed with real-time pcr.	a set of pcr primers was designed and validated for specific detection and quantification of prevotella ruminicola, prevotella albensis, prevotella bryantii, fibrobacter succinogenes, selenomonas ruminantium-mitsuokella multiacida, streptococcus bovis, ruminococcus flavefaciens, ruminobacter amylophilus, eubacterium ruminantium, treponema bryantii, succinivibrio dextrinosolvens, and anaerovibrio lipolytica. by using these primers and the real-time pcr technique, the corresponding species in the ...	2001	11375193
covalently linked polyamines in the cell wall peptidoglycan of selenomonas, anaeromusa, dendrosporobacter, acidaminococcus and anaerovibrio belonging to the sporomusa subbranch.		2002	12469301
activity and properties of fumarate reductase in ruminal bacteria.	fumarate-reducing bacteria were sought from the main ruminal bacteria. fibrobacter succinogenes, selenomonas ruminantium subsp. ruminantium, selenomonas ruminantium subsp. lactilytica, and veillonella parvula reduced fumarate by using h(2) as an electron donor. ruminococcus albus, prevotella ruminicola, and anaerovibrio lipolytica consumed fumarate, although they did not oxidize h(2). of these bacteria, v. parvula, two strains of selenomonas, and f. succinogenes had a high capacity to reduce fum ...	2000	12483585
changes in lactate-producing and lactate-utilizing bacteria in relation to ph in the rumen of sheep during stepwise adaptation to a high-concentrate diet.	changes in the numbers and types of lactate-producing and lactate-utilizing bacteria in the rumen of sheep were followed during stepwise adaptation from a low- to a high-concentrate diet. the mean numbers of bacteria increased after each change in diet when increasing amounts of maize grain were substituted for maize stover. a surge in number of amylolytic bacteria always preceded an increase in lactate-utilizing bacteria, and with the final diet containing 71% grain and molasses the two groups ...	1979	16345431
molecular characterization of sheep ruminal enrichments that detoxify pyrrolizidine alkaloids by denaturing gradient gel electrophoresis and cloning.	an enrichment of strictly anaerobic bacteria from ovine rumen fluid, which has previously been named l4m2, is known to detoxify animal hepatotoxins from the pyrrolizidine alkaloid family. these toxins are present in the tansy ragwort plant (senecio jacobaea). these plants have been described in livestock animals' range forages in regions of the world such as the northwest united states and south africa. the bacterial enrichment was characterized by molecular cloning techniques and by the molecul ...	2007	17345134
microbial ecology of the equine hindgut during oligofructose-induced laminitis.	alimentary carbohydrate overload is a significant cause of laminitis in horses and is correlated with drastic shifts in the composition of hindgut microbiota. equine hindgut streptococcal species (ehss), predominantly streptococcus lutetiensis, have been shown to be the most common microorganisms culturable from the equine caecum prior to the onset of laminitis. however, the inherent biases of culture-based methods are estimated to preclude up to 70% of the normal caecal microbiota. the objectiv ...	2008	18580970
biotransformation of 2,4,6-trinitrotoluene by pure culture ruminal bacteria.	twenty-one ruminal bacteria species were tested for their ability to degrade 2,4,6-trinitrotoluene (tnt) within 24 h. butyrivibrio fibrisolvens, fibrobacter succinogenes, lactobacillus vitulinus, selenomonas ruminantium, streptococcus caprinus, and succinivibrio dextrinosolvens were able to completely degrade 100 mg/l tnt, with <5% of the original tnt recovered as diaminonitrotoluene metabolites. eubacterium ruminantium, lactobacillus ruminis, ruminobacter amylophilus, streptococcus bovis, and w ...	2009	18839246
changes in process performance and microbial characteristics of retained sludge during low-temperature operation of an egsb reactor.	a lab-scale expanded granular sludge bed (egsb) reactor was seeded with granular sludge and operated to investigate the influence of temperature decrease on both process performance and the microbial community structure of the granular sludge. synthetic wastewater containing sucrose and volatile fatty acids was used as feed. the egsb reactor was brought online at a starting temperature of 15 degrees c and was reduced stepwise to a final temperature of 5 degrees c. the reactor exhibited sufficien ...	2008	18988102
effect of ph and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture.	milk fat depression in cows fed high-grain diets has been related to an increase in the concentration of trans-10 c(18:1) and trans-10,cis-12 conjugated linoleic acid (cla) in milk. these fatty acids (fa) are produced as a result of the alteration in rumen biohydrogenation of dietary unsaturated fa. because a reduction in ruminal ph is usually observed when high-concentrate diets are fed, the main cause that determines the alteration in the biohydrogenation pathways is not clear. the effect of p ...	2009	19700707
forage type and fish oil cause shifts in rumen bacterial diversity.	despite evidence supporting improved incorporation of beneficial polyunsaturated fatty acids (pufa) into ruminant products, such as meat and milk, following red clover and fish oil (fo) inclusion in the ruminant diet, little is known regarding the concomitant bacterial diversity. we evaluated the effects of feeding grass vs. red clover silage with incremental fo inclusion on known lipolytic, biohydrogenating, cellulolytic and proteolytic rumen bacterial communities of steers. following 14 days o ...	2010	20491929
effect of incremental levels of fish oil supplementation on specific bacterial populations in bovine ruminal fluid.	the objective of this study was to determine the effects of incremental replacement of dietary linoleic acid by >20-carbon polyunsaturated fatty acids (pufa) on changes in population of ruminal micro-organisms associated with fibre digestion and biohydrogenation using real-time pcr of bacterial 16s rrna sequences. four beef steers with ruminal cannulas were randomly assigned to control (ck, 65:35 forage to concentrate), ck with 3% sunflower oil plus 1% fish oil (s3f1), 2.5% sunflower oil plus 1. ...	2011	21198963
the effect of lipid supplements on ruminal bacteria in continuous culture fermenters varies with the fatty acid composition.	a single flow continuous culture fermenter system was used in this study to investigate the influence of dietary lipid supplements varying in their fatty acid content on the dna concentration of selected rumen bacteria. four continuous culture fermenters were used in a 4×4 latin square design with four periods of 10 d each. treatment diets were fed at 45 g/d (dm basis) in three equal portions during the day. the diets were: 1) control (con), 2) control with animal fat source (sat), 3) control wi ...	2011	21538241