Publications

methylocella palustris gen. nov., sp. nov., a new methane-oxidizing acidophilic bacterium from peat bogs, representing a novel subtype of serine-pathway methanotrophs.	a new genus, methylocella, and a new species, methylocella palustris, are proposed for three strains of methane-oxidizing bacteria isolated from acidic sphagnum peat bogs. these bacteria are aerobic, gram-negative, colourless, non-motile, straight and curved rods that utilize the serine pathway for carbon assimilation, multiply by normal cell division and contain intracellular poly-beta-hydroxybutyrate granules (one at each pole). these strains use methane and methanol as sole sources of carbon ...	2000	10843033
characterization of methanotrophic bacteria on the basis of intact phospholipid profiles.	the intact phospholipid profiles (ipps) of seven species of methanotrophs from all three physiological groups, type i, ii and x, were determined using liquid chromatography/electrospray ionization/mass spectrometry. in these methanotrophs, two major classes of phospholipids were found, phosphatidylglycerol (pg) and phosphatidylethanolamine (pe) as well as its derivatives phosphatidylmethylethanolamine (pme) and phosphatidyldimethylethanolamine (pdme). specifically, the type i methanotrophs, meth ...	2000	10913867
detection and enumeration of methanotrophs in acidic sphagnum peat by 16s rrna fluorescence in situ hybridization, including the use of newly developed oligonucleotide probes for methylocella palustris.	two 16s rrna-targeted oligonucleotide probes, mcell-1026 and mcell-181, were developed for specific detection of the acidophilic methanotroph methylocella palustris using fluorescence in situ hybridization (fish). the fluorescence signal of probe mcell-181 was enhanced by its combined application with the oligonucleotide helper probe h158. mcell-1026 and mcell-181, as well as 16s rrna oligonucleotide probes with reported group specificity for either type i methanotrophs (probes m-84 and m-705) o ...	2001	11571193
methylocapsa acidiphila gen. nov., sp. nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from sphagnum bog.	a novel genus and species, methylocapsa acidiphila gen. nov., sp. nov., are proposed for a methane-oxidizing bacterium isolated from an acidic sphagnum peat bog. this bacterium, designated strain b2t, represents aerobic, gram-negative, colourless, non-motile, curved coccoids that form conglomerates covered by an extracellular polysaccharide matrix. the cells use methane and methanol as sole sources of carbon and energy and utilize the serine pathway for carbon assimilation. strain b2t is a moder ...	2002	11837310
[methanotrophs of the psychrophilic microbial community of the russian arctic tundra].	in tundra, at a low temperature, there exists a slowly developing methanotrophic community. methane-oxidizing bacteria are associated with plants growing at high humidity, such as sedge and sphagnum; no methonotrophs were found in polytrichous and aulacomnious mosses and lichens, typical of more arid areas. the methanotrophic bacterial community inhabits definite soil horizons, from moss dust to peat formed from it. potential ability of the methanotrophic community to oxidize methane at 5 degree ...	2002	12244726
[methanotrophic bacteria of acid sphagnum bogs].	acid sphagnum bogs cover a considerable part of the territory of russia and are an important natural source of biogenic methane, which is formed in their anaerobic layers. a considerable portion of this methane is consumed in the aerobic part of the bog profile by acidophilic methanotrophic bacteria, which comprise the methane filter of sphagnum bogs and decrease ch4 emission to the atmosphere. for a long time, these bacteria escaped isolation, which became possible only after the elucidation of ...	2002	12526194
methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol.	two strains of gram-negative, aerobic, non-pigmented, non-motile, rod-shaped, methane-oxidizing bacteria were isolated from an acidic forest cambisol near marburg, germany, and were designated as strains bl2(t) and a1. these bacteria were morphologically and phenotypically similar to methylocella palustris k(t). the cells possess a highly specific bipolar appearance. they lack the intracytoplasmic membranes common to all methane-oxidizing bacteria except methylocella, but contain a vesicular mem ...	2003	13130000
methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands.	a novel species, methylocella tundrae, is proposed for three methanotrophic strains (t4t, tch1 and ty1) isolated from acidic sphagnum tundra peatlands. these strains are aerobic, gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. strains t4t, tch1 and ty1 are moderately acidophilic organisms capable of growth between ph 4.2 and 7.5 (optimum 5.5-6.0) and between 5 and 30 degrees c (optimum 15 degre ...	2004	14742473
aerobic methanotrophic bacteria of cold ecosystems.	this review summarizes the recent advances in understanding the ecophysiological role and structure-function features of methanotrophic bacteria living in various cold ecosystems. the occurrence of methanotrophs in a majority of psychrosphere sites was verified by direct measurement of their methane-utilizing activity, by electron microscopy and immunofluorescent observations, and analyses of specific signatures in cellular phospholipids and total dnas extracted from environmental samples. surpr ...	2005	16329925
differential detection of type ii methanotrophic bacteria in acidic peatlands using newly developed 16s rrna-targeted fluorescent oligonucleotide probes.	abstract based on an extensive 16s rrna sequence database for type ii methanotrophic bacteria, a set of 16s rrna-targeted oligonucleotide probes was developed for differential detection of specific phylogenetic groups of these bacteria by fluorescence in situ hybridisation (fish). this set of oligonucleotides included a genus-specific probe for methylocystis (mcyst-1432) and three species-specific probes for methylosinus sporium (msins-647), methylosinus trichosporium (msint-1268) and the recent ...	2003	19719661