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[effect of carbon and nitrogen sources on the cellulolytic activity of trametes trogii].trametes trogii was grown in a liquid synthetic medium containing different carbon and nitrogen sources. enzymatic activities of cellulases (endoglucanase, exoglucanase and beta-glucosidase) were measured in culture supernatants. organic nitrogen sources were the most favourable for growth and cellulase production. increasing nitrogen concentrations also increased cellulase production. among carbon sources, crystalline cellulose, cellobiose and a mixture of carboxymethylcellulose and cellobiose ...19957568864
characterization of extracellular proteases from trametes trogii.the peptidase activities excreted in culture broths of trametes trogii mycelium have been identified by determining the digestion pathway of various peptides. insulin beta-chain (30 residues), procasomorphin (10 residues) and two peptides of five residues (proctolin and thymosin alpha 1 fragment 23-27) were utilized as model substrates. aminopeptidase, carboxypeptidase, endopeptidase and dipeptidyl aminopeptidase activities were revealed and information on their specificity was deduced. prelimin ...19968821431
[effect of culture conditions on the production of cellulases by trametes trogii].production of the components of the cellulase complex by trametes trogii was investigated under different culture conditions. endoglucanase, exoglucanase and beta-glucosidase were produced by trametes trogii under shaken and stationary conditions using crystalline cellulose as the only carbon source. no marked differences were found for endo- and exoglucanase production between both culture conditions. in shaken cultures beta-glucosidase values doubled those obtained in stationary conditions. al ...19979229721
laccase from the white-rot fungus trametes trogii.the white-rot fungus trametes trogii excretes a main laccase showing a molecular mass of 70 kda, acidic isoelectric point and n-terminal sequence homologous to that of several phenol oxidases. the purified enzyme oxidizes a number of phenolic and non-phenolic compounds; recalcitrant molecules may be converted into substrates by introducing, in the correct position, o- or p-orienting ring-activating groups.19989650252
fungal degradation of lipophilic extractives in eucalyptus globulus woodsolid-state fermentation of eucalypt wood with several fungal strains was investigated as a possible biological pretreatment for decreasing the content of compounds responsible for pitch deposition during cl2-free manufacture of paper pulp. first, different pitch deposits were characterized by gas chromatography (gc) and gc-mass spectrometry (ms). the chemical species identified arose from lipophilic wood extractives that survived the pulping and bleaching processes. second, a detailed gc-ms ana ...199910103223
molecular phylogeny of trametes and related genera.mitochondrial small subunit ribosomal dnas were sequenced and phylogenetically analyzed for trametes and related genera of ryvarden's trametes group. parsimony and distance analyses indicated that most of trimitic genera with white rotting habit of the trametes group clustered within a single clade, suggesting that they may have originated from a common ancestor. the phylogenetic position and generic placement of trametes consors was uncertain as yet. trametes trogii grouped with coriolopsis gal ...199910427407
effect of spent cotton stalks on color removal and chemical oxygen demand lowering in olive oil mill wastewater by white rot fungi.wastewater from olive oil mill was decolorized (and its chemical oxygen demand reduced in static cultivation) using the fungi coriolus versicolor, funalia trogii, phanerochaete chrysosporium and pleurotus sajor-caju. the effect of cotton stalk on decolorizing and cod removing capability was demonstrated. p. chrysosporium (in 20% medium with cotton stalk) reduced the cod by 48% and color by 58%, f. trogii (in 30% medium with cotton stalk)) by 51 and 55%, respectively.199911097026
relationship between production of 3-indoleacetic acid and peroxidase-laccase activities depending on the culture periods in funalia trogii (trametes trogii).the relationship between production of 3-indoleacetic acid (iaa) and peroxidase and laccase activity was investigated in white-rot fungus funalia trogii (trametes trogii). f. trogii produced iaa and peroxidase and laccase as both primary metabolite and secondary metabolite; the levels of iaa may be influenced by peroxidase and laccase. a correlation exists between the levels of iaa and peroxidase-laccase activity.200111501398
industrial and agricultural wastes as substrates for laccase production by white-rot fungi.white-rot fungi, coriolus versicolor and funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation.200111501400
decolorization of orange ii dye by white-rot fungi.agitation, temperature, inoculum size, initial ph and ph of buffered medium affected the decolorization of orange ii dye by coriolus versicolor and funalia trogii. the optimum temperature and initial ph value for decolorization were 30 degrees c and 6.5-7.0, respectively; ph 4.5 was the most efficient in buffered cultures. high decolorization extents were reached at all agitation rates. at an inoculum size of more than 1 ml, the extent of decolorization changed only slightly. high extents were o ...200111501402
structural and kinetic characterization of native laccases from pleurotus ostreatus, rigidoporus lignosus, and trametes trogii.a comparative study has been performed on five native laccases purified from the three basidiomycete fungi pleurotus ostreatus, rigidoporus lignosus, and trametes trogii to relate their different catalytic capacities to their structural properties. spectroscopic absorption features and epr spectra at various ph values of the five enzymes are very similar and typical of the blue oxidases. the analysis of the dependence of kinetic parameters on ph suggested that a histidine residue is involved in ...200111565899
decolourisation of the textile dye astrazon red fbl by funalia trogii pellets.the effects of various conditions such as initial ph, dye concentrations, amount of pellet, temperature and agitation on decolourising activity of funalia trogii were investigated. these, except initial ph, were all found to be important for dye decolourising activity of f. trogii. the decolourisation of the dye involved adsorption of the dye compound by fungal pellets at the initial stage, followed by the decolourisation through microbial metabolism. heat-killed pellets were also tested for the ...200211762908
[degradation of anthraquinone blue by trametes trogii].the ability of the white rot fungus trametes trogii bafc 463 (high producer of ligninolytic enzymes, especially laccase and manganese peroxidase) to degrade the dye anthraquinone blue, refractory to bacterial attack, was evaluated. both tropho- and idiophasic t. trogii cultures in synthetic medium (glucose/asparagine) and complex medium (malt extract/glucose) were able to transform up to 88% dye in 4 hours. the activity of laccase, an oxygen-dependent phenoloxidase which was present at high leve ...200111833254
[effect of cocultivating fungal species on the degradation of lignocellulose residues].the degradation potential of phanerochaete sordida, trametes trogii, coprinus truncorum and paecilomyces sp. upon yard wastes was evaluated. the species had been inoculated individually or in pairs formed by p. sordida and paecilomyces sp., t. trogii and paecilomyces sp., and c. truncorum and paecilomyces sp. the highest level of endoxilanase activity was produced by p. sordida growing alone, during day 21 (1.09 u/g of dry material), but in p. sordida and paecilomyces sp. cultures, the detected ...200211942082
effect of synthetic and natural culture media on laccase production by white rot fungi.laccase is among the major enzymes of white rot fungi involved in lignocellulose degradation. the present paper reports its production by two white rot fungi (coriolus versicolor, funalia trogii) under different nutritional conditions. various synthetic culture media and natural culture medium (molasses wastewater) were tested. enzyme production in various synthetic culture media, molasses wastewater (vinasse) culture medium and in the absence or presence of cotton stalk supplements showed that ...200211991068
degradation of environmental pollutants by trametes trogii.the ability of the ligninolytic fungus trametes trogii to degrade in vitro different xenobiotics (pcbs, pahs and dyes) was evaluated. either 200 ppm of a pcb mixture (aroclor 1150) or 160 ppm of an industrial pah mixture (10% v/v of pahs, principal components hexaethylbenzene, naphthalene, 1-methyl naphthalene, acenaphthylene, anthracene, fluorene and phenanthrene), were added to trophophasic and idiophasic cultures growing in a nitrogen limited mineral medium (glucose/asparagine) and in a compl ...200212415898
effect of paraquat on cellular defense enzymes and glutathione level of funalia trogii.the effect of paraquat on the activities of antioxidant defense and detoxifying enzymes of the white-rot fungus funalia trogii was determined. paraquat increased the activities of glutathione reductase (gr), glutathione transferase (gt) and superoxide dismutase at 1 mmol/l, while at 0.1 mmol/l it did not affect the activity of gr and gt. it depressed the catalase activity and the amount of glutathione at all concentrations used. paraquat treatment probably depresses antioxidant defense component ...200212422520
dibenzyl sulfide metabolism by white rot fungi.microbial metabolism of organosulfur compounds is of interest in the petroleum industry for in-field viscosity reduction and desulfurization. here, dibenzyl sulfide (dbs) metabolism in white rot fungi was studied. trametes trogii uamh 8156, trametes hirsuta uamh 8165, phanerochaete chrysosporium atcc 24725, trametes versicolor ifo 30340 (formerly coriolus sp.), and tyromyces palustris ifo 30339 all oxidized dbs to dibenzyl sulfoxide prior to oxidation to dibenzyl sulfone. the cytochrome p-450 in ...200312571066
ribosomal dna sequence analysis shows that the basidiomycete c30 belongs to the genus trametes.the basidiomycete c30 was considered as an isolate of a population of marasmius quercophilus collected on evergreen oak litter from the mediterranean forest. recent phenotypic studies have clearly shown that it differs from newly characterized m. quercophilus isolates. subsequent analysis of laccase genes revealed that c30 sequences are similar to laccase encoding sequences from organisms belonging to the polyporoid clade. comparison of sequences of the c30 its regions, including 5.8s rdna, with ...200312576155
primary structure and transcription analysis of a laccase-encoding gene from the basidiomycete trametes trogii.a cdna coding for laccase was isolated from the white-rot fungus trametes trogii 201. this cdna corresponded to the lcc1 gene, which coded for a precursor protein of 517 amino acids with a 21 amino acid signal peptide. comparison of the deduced sequence with known laccases showed that this enzyme was most closely related to lac1 from basidiomycete pm1 and trametes c30 (98% similarity). the expression of lcc1 was analysed under different growth conditions; transcription of this gene was enhanced ...200313680201
decolorization and bioremediation of molasses wastewater by white-rot fungi in a semi-solid-state condition.molasses wastewater (vinasse; the by-product of distillation of fermented sugar) was decolorized and its chemical oxygen demand (cod) was reduced in static cultivation using the fungi coriolus versicolor, funalia trogii, phanerochaete chrysosporium and pleurotus pulmonarius ('pleurotus sajorcaju'). the effect of cotton stalk on decolorizing and cod removing capability of four fungi was determined. in the entire concentration range tested (10-30%), wastewater was effectively decolorized by c. ver ...200314533485
optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology.a box-wilson central composite design was applied to optimize copper, veratryl alcohol and l-asparagine concentrations for trametes trogii (bafc 212) ligninolytic enzyme production in submerged fermentation. decolorization of different dyes (xylidine, malachite green, and anthraquinone blue) by the ligninolytic fluids from the cultures was compared. the addition of copper stimulated laccase and glyoxal oxidase production, but this response was influenced by the medium n-concentration, with impro ...200314648345
biosorption of hg2+, cd2+, and zn2+ by ca-alginate and immobilized wood-rotting fungus funalia trogii.funalia trogii biomass was immobilized in ca-alginate gel beads. the live and heat inactivated immobilized forms were used for the biosorption of hg2+, cd2+ and zn2+ ions by using plain ca-alginate gel beads as a control system. the effect of ph was investigated and the maximum adsorption of metal ions on the ca-alginate and both live and inactivated immobilized fungal preparations were observed at ph 6.0. the temperature change between 15 and 45 degrees c did not affect the biosorption capacity ...200415177759
astrazon red dye decolorization by growing cells and pellets of funalia trogii.the dye decolorization activity of fungal pellets has been compared with another method based on the decolorization of dye by growing cells. the pellet method was more advantageous than the growing cell method. the growing cells of f. trogii decolorized 21% of the dye in distilled water medium and 16% in stock basal medium in 24 h. on the other hand, funalia trogii pellets rapidly decolorized the astrazon red dye, mono-azo textile dye, in 24 h, without any visual sorption of any dye to the pelle ...200415266597
role of white rot fungus funalia trogii in detoxification of textile dyes.toxic and genotoxic effects of the textile dyes on organisms suggest the need for remediation of dyes before discharging them into the environment. for this reason, the ability of funalia trogii pellets to detoxify textile dyes was investigated and evaluated. although, textile dyes are toxic substances for many microorganisms, the pellets were able to decolorize and detoxify the azo dyes used. astrazon blue and red dyes inhibit growth of f. trogii and s. aureus on solid medium in a concentration ...200515812863
level of heavy metals in some edible and poisonous macrofungi from batman of south east anatolia, turkey.the macrofungi specimens were collected and identified from locations around batman province of turkey. a group of 21 macrofungi were collected from 29 different locations and analysed for the heavy metal contents. the highest pb and cu levels were found in russula rubroalba while the highest cd level was determined for the species agaricus xanthodermus. fe level was maximum for the species funalia trogii; mn for mycena personsii; zn level was for morchella esculenta and co level was for the spe ...200415847333
decolourisation of reactive textile dyes drimarene blue x3lr and remazol brilliant blue r by funalia trogii atcc 200800.decolourisation of reactive dyes drimarene blue x3lr and remazol brilliant blue r by white rot fungi funalia trogii was studied under static conditions. the effect of various conditions such as mycelial age, initial dye and glucose concentrations on decolourisation were also investigated. decolourisation activity of f. trogii was compared with phanerochaete chrysosporium known as test microorganism. it was found that 7-day-old cultures were more effective than 5-day-old cultures of f. trogii for ...200515865144
evaluation of cytotoxic and mutagenic effects of coriolus versicolor and funalia trogii extracts on mammalian cells.this study examined the in vitro cytotoxic activities of standardized aqueous bioactive extracts prepared from coriolus versicolor and funalia trogiiatcc 200800 on hela and fibroblast cell lines using a mtt (3-[4,5-dimetiltiazol-2-]-2-5-difeniltetrazolium bromide) cytotoxicity assay. f. trogii and c. versicolor extracts were cytotoxic to both cell lines. at 10 microl treatment level, f. trogii and c. versicolor extracts inhibited proliferation of hela cancer cells by 71.5% and 45%, respectively, ...200616455591
adsorptive removal of textile dyes from aqueous solutions by dead fungal biomass.dead fungal biomass prepared from phanerochaete chrysosporium and funalia trogii was tested for their efficiency in removal of textile dyes. the effects of contact time, initial dye concentration, amount of dead biomass and agitation rate on dye removal have been determined. removal of all dyes required a very short time (60 min). experimental results show that, p. chrysosporium was more effective than f. trogii . an increase in the amount of dead biomass positively affected of the dye removal. ...200616463316
remazol brilliant blue r decolourization by the laccase from trametes trogii.the decolourization of the recalcitrant dye rbbr by the culture filtrate of trametes trogii and its isolated laccase was investigated. both filtrates from cu-induced cultures as well as purified laccase decolourized the dye rbbr. the purified laccase decolourized the dye down to 97% of 100 mg l(-1) initial concentration of rbbr when only 0.2u ml(-1) of laccase was used in the reaction mixture. the effects of different physicochemical parameters were tested and optimal decolourization rates occur ...200616473395
heterologous expression of lcc1 gene from trametes trogii in pichia pastoris and characterization of the recombinant enzyme.fungal laccases are useful enzymes for industrial applications; they exhibit broad substrate specificity and thus are able to oxidize a variety of xenobiotic compounds including chlorinated phenolics, synthetic dyes, pesticides and polycyclic aromatic hydrocarbons. unfortunately, the biotechnological exploitation of laccases can be hampered by the difficulties concerning the enzyme production by the native hosts.200617038162
detoxification of tunisian landfill leachates by selected fungi.young landfill leachates (lfl) collected from djebel chekir (tunisia) discharge area were found to be highly loaded with organic matter, ammonia, salts, heavy metals, phenols and hydrocarbons. despite the possibility of their biodegradability, they represent a threat to the environment and show some resistance to conventional wastewater treatment processes. for these reasons, this study attempted to develop a biological process for the treatment of lfl using selected strains of trametes trogii, ...200817570582
fungal laccase, cellobiose dehydrogenase, and chemical mediators: combined actions for the decolorization of different classes of textile dyes.dyes belonging to the mono-, di-, tri- and poly-azo as well as anthraquinonic and mono-azo cr-complexed classes, chosen among the most utilized in textile applications, were employed for a comparative enzymatic decolorization study using the extracellular crude culture extracts from the white rot fungus funalia (trametes) trogii grown on different culture media and activators able to trigger different levels of expression of oxidizing enzymes: laccase and cellobiose dehydrogenase. laccase contai ...200818281211
biosorption of phenol and 2-chlorophenol by funalia trogii pellets.the removal of phenol (ph) and 2-chlorophenol (2-cph) from aqueous solution by native and heat inactivated fungus funalia trogii pellets were investigated. the effects of contact time, solid/liquid ratio, optimum ph and temperature on the phenols removal capacity by the pellets were established. the removal efficiency of phenols increased significantly with increasing biomass dose. the optimum ph was detected to be 8.0. the second-order equations are described and evaluated on the basis of a com ...200919186052
optimization of recombinant fungal laccase production with strains of the yeast kluyveromyces lactis from the pyruvate decarboxylase promoter.laccases are multicopper oxidases of wide specificity that catalyze the oxidation of phenolic and related compounds using molecular oxygen as the electron acceptor. here, we report the production of the lcc1 laccase of the fungus trametes trogii in strains of the yeast kluyveromyces lactis, using the pyruvate decarboxylase promoter (klpdc1) as an expression system. we assayed laccase production in various strains, with replicative and integrative transformants and with different cultivation para ...200919527303
modeling the 3-d structure of a recombinant laccase from trametes trogii active at a ph close to neutrality.a cdna encoding a novel laccase from the white-rot fungus trametes trogii was cloned and expressed in pichia pastoris. the recombinant protein (lcc2) exhibited kinetic parameters for both phenolic and non phenolic substrates that were different from the previously described lcc1, the main laccase isoform expressed by t. trogii; in addition, the ph/activity profiles for phenolic substrates of lcc2 were shifted upward by 1-1.5 ph units towards neutrality as compared to lcc1. comparative modeling o ...200919806440
decolourization and detoxification of textile industry wastewater by the laccase-mediator system.decolourization and detoxification of a textile industry effluent by laccase from trametes trogii in the presence and the absence of laccase mediators was investigated. laccase alone was not able to decolourize the effluent efficiently even at the highest enzyme concentration tested: less than 10% decolourization was obtained with 9 u/ml reaction mixture. to enhance effluent decolourization, several potential laccase mediators were tested at concentrations ranging from 0 to 1mm. most potential m ...201019945786
effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi. dye decolorization by selected culture filtrates.the effect of amino acids, complex nitrogen sources and vitamin addition on trametes trogii, trametes villosa and coriolus versicolor var. antarcticus ligninolytic enzyme production, was evaluated. dye decolorization by their culture filtrates was compared. glutamic acid followed by peptone, were the best n sources for laccase and manganese peroxidase production. the three fungi produced two laccase isoenzymes (molecular weights from 38 up to 150 kda); their pattern of production was not affecte ...201020153961
[enhancement of laccase activity by combining white rot fungal strains].the method of combining white rot fungal strains was used to enhance laccase activity, and the interaction mechanism between strains was also studied. the laccase activity of combined fungi of strain 55 (trametes trogii) and strain m-6 (trametes versicolor) were 24.13 and 4.07-fold higher than that of strain 55 and strain m-6, respectively. no inhibitory effect was observed when the two strains were co-cultivated. on plate cultivation, there was hyphal interference in the contact area, where lac ...201020391719
biochemical and molecular characterization of coriolopsis rigida laccases involved in transformation of the solid waste from olive oil production.two laccase isoenzymes were purified and characterized from the basidiomycete coriolopsis rigida during transformation of the water-soluble fraction of "alpeorujo" (wsfa), a solid residue derived from the olive oil production containing high levels of toxic compounds. zymogram assays of laccases secreted by the fungus growing on wsfa and wsfa supplemented with glucose showed two bands with isoelectric points of 3.3 and 3.4. the kinetic studies of the two purified isoenzymes showed similar affini ...201020607234
layer-by-layer self-assembled osmium polymer-mediated laccase oxygen cathodes for biofuel cells: the role of hydrogen peroxide.high potential purified trametes trogii laccase has been studied as a biocatalyst for oxygen cathodes composed of layer-by-layer self-assembled thin films by sequential immersion of mercaptopropane sulfonate-modified au electrode surfaces in solutions containing laccase and osmium-complex bound to poly(allylamine), (pah-os). the polycation backbone carries the os redox relay, and the polyanion is the enzyme adsorbed from a solution of a suitable ph so that the protein carries a net negative char ...201020698679
copper induction of lignin-modifying enzymes in the white-rot fungus trametes trogii.trametes trogii, a white rot basidiomycete involved in wood decay worldwide, produces several ligninolytic enzymes, laccase being the dominant one, with higher titers than those reported for most other white rot fungi studied up to date. the effect of copper on in vitro production of extracellular ligninolytic activities was studied. cuso(4)·5h(2)o concentrations from 1.6 μm to 1.5 mm were tested in a synthetic medium with glucose 20 g/l and asparagine 3 g/l. the addition of copper (up to 1 mm) ...200221156508
comparison of antioxidant capacities of ganoderma lucidum (curtis) p. karst and funalia trogii (berk.) bondartsev & singer by using different in vitro methods.the aim of the study was to investigate antioxidant activities of ganoderma lucidum and funalia trogii. ethanol and water crude extracts from g. lucidum and f. trogii were investigated for their antioxidant capacity in some different assays, namely, the 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging activity, metal chelating activity against ferrous ions, and plasma lipid peroxidation inhibitory. in addition, the amounts of total phenol, ascorbic acid, ß-carotene, and lycopene component ...201121314365
decolorization of synthetic dyes by crude laccase from a newly isolated trametes trogii strain cultivated on solid agro-industrial residue.a new dye-decolorizing white-rot fungus was isolated and identified as trametes trogii based on its its-5.8s rrna gene sequence analysis and morphological characteristics. laccase was the only lignolytic enzyme produced by this strain during solid substrate fermentation (ssf) in soybean cake, a solid agro-industrial residue used for the first time in enzyme production. the extracellular crude enzyme from t. trogii in solid substrate fermentation showed good activity in synthetic dye color remova ...201121315513
a study of anti-cancer effects of funalia trogii in vitro and in vivo.extracts of natural products have been used for many years for health benefits. we report on an in vitro and in vivo study into the anti-tumour efficacy of an aqueous extract of the mycelial form of basidiomycete, funalia trogii. a variety of biological assays were used to show that a 4h exposure of ht29, lncap, pc3, mcf-7 and mda-mb-231 tumour cells to extract (0.5-5.0 mg/ml) resulted in significant cytotoxicity. in a clonogenic assay, ic50 values were found to range from 0.4-0.72 mg/ml; exposi ...201121333711
efficient removal of pollutants from olive washing wastewater in bubble-column bioreactor by trametes versicolor.the white-rot fungi panus tigrinus, funalia trogii and trametes versicolor have been tested in shake flasks for the reduction of olive washing wastewater (oww) pollutants and production of oxidases on oww-based media. p. tigrinus was rejected for its scarce performance. f. trogii showed best production of laccase (27000ug(-1)), while t. versicolor appeared a good pollutant degrader reducing colour, cod and phenols by 60, 72 and 87%, respectively. only t. versicolor grew well in bubble-column bio ...201121524782
differential decolorization of textile dyes in mixtures and the joint effect of laccase and cellobiose dehydrogenase activities present in extracellular extracts from funalia trogii.the largest part of the bio-decolorization investigations have been performed to date on a single dye without exploring the behavior in complex mixtures as the real dyeing baths. therefore, mixtures of dyes belonging to azo and anthraquinonic classes, chosen among the most utilized in textile wool dyeing, were employed for comparative enzymatic decolorization studies using the extracellular extracts from the white rot fungus funalia trogii, to understand how the concomitant presence of more than ...201122112619
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