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[laboratory and field tests about the transmission of erysimum latent virus by phyllotreata species (chrsomelidae) (author's transl)].erysimum latent virus (elv) proved to be transmissible by phyllotreta atra, p. nemorum, p. nigripes, and p. undulata. from them p. nemorum is a new discovered vector. the acquisition of the virus by the beetles was possible within a feeding time of less than 10 minutes. because virus transmission could be performed within 30 to 60 minutes immediately after virus uptake there exists apparently no latent period in the vector. the retention or persistence of elv in the phyllotreta species amounted ...1977565119
broccoli necrotic yellows virus in cauliflower and in the aphid, brevicoryne brassicae l. 1977841850
the fine structure of distal receptors on the labium of the aphid, brevicoryne brassicae l. (homoptera). implications for current theories of sensory transduction.short peg receptors located at the distal tip of the aphid labium have the structure of mechanoreceptors. each peg is innervated by a single sensory nerve which is anchored eccentrically to a basal cuticular tube and terminates in electron-dense material in the base of the peg. the arrangement and eccentric insertion of the eight pegs in the labial wall on one side of the stylet groove, with the eccentric insertions of their innervating neurones, provide a mirror image of the receptors on the op ...1977884713
comparison of short- and long-feed transmission of the cauliflower mosaic virus cabb-s strain and s delta ii hybrid by two species of aphid: myzus persicae (sulzer) and brevicoryne brassicae (l.).the cauliflower mosaic virus (camv) hybrid s delta ii, partially deleted in orfii, loses its transmissibility by the aphid myzus persicae on 5-min acquisition feed. we have also shown that it is not transmitted after 8-h acquisition feed. the same occurs with brevicoryne brassicae. therefore, the aphid transmission factor (atf) is involved in both means of transmission and in both aphid species. m. persicae can acquire camv cabb-s strain in less than 20 s. m. persicae is a more efficient vector ...20062087600
composition of the ribonucleic acid of alate cabbage aphids brevicoryne brassicae (l.). 19644157672
sensory innervation monitoring movement and position in the mandibular stylets of the aphid, brevicoryne brassicae. 19744837746
the fine structure of the mycetome symbiotes of the aphids brevicoryne brassicae, myzus persicae, and macrosiphum rosae. 19714999588
endocrine aspects of alary polymorphism in brevicoryne brassicae l. 19715317419
cabbage aphid: effect of isolation on form and on endocrine activity.isolated wingless cabbage aphids, brevicoryne brassicae (l.), produced only wingless young. aphids isolated from birth all became wingless, whereas over 50 percent of grouped controls developed wings. isolation caused hyperactivity of the corpus allatum; this hyperactivity may have caused the wingless form.19685634919
feeding behaviour and susceptibility of myzus persicae (sulz) and brevicoryne brassicae (l.) to [14c] phorate in artificial diet.the effect of artificial diet on the growth and reproduction of the aphids myzus persicae (sulz) and brevicoryne brassicae (l) was studied. following the application of [14c] phorate to the diet, the results showed that b. brassicae was more susceptible than m. persicae. in these experiments the dead and live aphids and their honeydew were also analysed. the dead aphids contained more toxic 14c than the live ones with a direct correlation between the amount of insecticide in the diet and the ins ...19806155883
toxicity of [14c] phorate applied systemically to the aphids myzus persicae (sulz) and brevicoryne brassicae (l.) grown on brussels sprout plants.the effect of foliar application of [14c] phorate on the larvae of m. persicae and b. brassicae was studied. regression analysis of the toxic components in the foliage and the probit kill showed that b. brassicae was more susceptible to the insecticide than m. persicae. the level of radioactivity extracted from the leaves, aphids and their honeydew shows that there was no correlation between the applied dose and the mortality. the amounts of honeydew produced by colonies before and after applica ...19806159846
purification and characterisation of a non-plant myrosinase from the cabbage aphid brevicoryne brassicae (l.).plant myrosinases and glucosinolates constitute a defence system in cruciferous plants towards pests and diseases. we have purified for the first time a non-plant myrosinase from the cabbage aphid brevicoryne brassicae (l.) to homogeneity. the protein was n-terminally blocked and protease (trypsin and lys c) degradation gave peptides of which five were sequenced. the protein is a dimer with subunits of mass 54 kda+/-500 da. western blot analysis with an anti-aphid myrosinase antibody showed a st ...200111102829
purification and characterization of myrosinase from the cabbage aphid (brevicoryne brassicae), a brassica herbivore.aphids are among the most serious insect pests of agricultural crops in the world. they often have specific hosts, and the cabbage aphid (brevicoryne brassicae) is a specialist on cruciferae. it has previously been described that certain insects contain the enzyme myrosinase (ec 3.2.3.1), which is considered an important defence enzyme of crucifers. myrosinase was purified to homogeneity from cabbage aphid soluble extracts using anion-exchange and phenyl-sepharose chromatography. the protein has ...200111179970
[behavior response of four leis axyridis varieties to volatiles from tea and toxoptera aurantii].the behavior response of the adults of four leis axyridis varieties to kairomone from taxoptera aurantii was determined by y-shape olfaction instrument. the results showed that when supplied with two-two-combinations of taxoptera aurantii, brevicoryne brassicae and lipaphis erysimi, tea aphids plus aphid-damaged vs. normal shoots, and aphid-damaged vs. normal shoots, leis axyridis had a preference for tea aphids, tea aphids plus aphid-damaged tea shoots, or aphid-damaged tea shoots. there existe ...200011767644
spatial organization of the glucosinolate-myrosinase system in brassica specialist aphids is similar to that of the host plant.secondary metabolites are important in plant defence against pests and diseases. similarly, insects can use plant secondary metabolites in defence and, in some cases, synthesize their own products. the paper describes how two specialist brassica feeders, brevicoryne brassicae (cabbage aphid) and lipaphis erysimi (turnip aphid) can sequester glucosinolates (thioglucosides) from their host plants, yet avoid the generation of toxic degradation products by compartmentalizing myrosinase (thioglucosid ...200211798435
characterization and evolution of a myrosinase from the cabbage aphid brevicoryne brassicae.the aphid myrosinase gene has been elucidated using rapid amplification of cdna ends-pcr. sequencing has shown that aphid myrosinase has significant sequence similarity (35%) to plant myrosinases and other members of glycosyl hydrolase family 1 (ghf1). the residues acting as proton donor and nucleophile, in the hydrolysis of glucosinolates by aphid myrosinase, are identified as glu 167 and glu 374 respectively. the equivalent residues in plant myrosinase are gln 187 and glu 409 and for the cyano ...200211804799
effect of cabbage cultivars with varying levels of resistance to aphids on the performance of the parasitoid, aphidius colemani (hymenoptera: braconidae).the effect of aphid resistance in plant cultivars on parasitism of myzus persicae (sulzer) and brevicoryne brassicae (linnaeus) by aphidius colemani viereck was investigated under laboratory conditions using three cultivars of common cabbage, brassica oleracea var. capitata. significantly greater aphid populations were found on cv. derby day (green-leaved, susceptible to both aphid species), regardless of the presence or absence of parasitoids, compared with cv. minicole (green-leaved, partially ...200212020362
tritrophic interactions between cabbage cultivars with different resistance and fertilizer levels, cruciferous aphids and parasitoids under field conditions.tritrophic interactions involving cabbage brassica oleracea var. capitata cultivars +/- fertilizer, brevicoryne brassicae (linnaeus) and myzus persicae (sulzer), and the parasitoids diaeretiella rapae (m'cintosh) and aphidius sp. were conducted in 1998 and 1999. brevicorne brassicae was the dominant aphid species on all cultivars +/- fertilizer, except for some treatments in late season 1998. ruby ball (red-leaved with antixenosis factors for b. brassicae alates) +/- fertilizer was consistently ...200212020363
characterisation of aphid myrosinase and degradation studies of glucosinolates.myrosinase from brevicoryne brassicae was purified by ammonium sulfate fractionation, dialysis, and chromatography on a deae column. the chromatography yielded a single peak and a 115.6-fold purification. further fplc gel filtration gave a single peak at 120 kda. denaturing sds/page of the protein revealed a single band at 60 kda, indicating that the native b. brassicae myrosinase is a dimer. kinetic parameters towards 8 glucosinolates were calculated. strong differences of v(max) and k(m) were ...200212125058
aphid prey suitability as environmental effect on adalia bipunctata reproduction.secondary plant metabolites (allelochemicals) play a major role in plant-insect interactions. glucosinolates (gls) and their degradation products from brassica species are attractants and feeding stimulants for brassicaceae specialist insects but are generally repellent and toxic for generalist herbivores. the impact of these compounds on crucifer specialist insects are well known but their effect on generalist predators is still not well documented. prey host plant influence on reproduction of ...200212696423
feeding behaviour of the aphids myzus persicae and brevicoryne brassicae, studied with radiophosphorus. 195313051326
host-associated genetic structure of mexican populations of the cabbage aphid brevicoryne brassicae l. (homoptera: aphididae).phytophagous insects can use different host plant species across their geographic distribution. within a locality, however, their feeding can be restricted to one or two plant species. if host species constitute different selective regimes to herbivorous insects, genetic differentiation and host-associated local adaptation may occur. in this study, we describe the genetic structure of the aphid brevicoryne brassicae l. associated to brassica campestris l. and b. oleraceae var. capitata l., two p ...200314512958
effects of allelochemicals from first (brassicaceae) and second (myzus persicae and brevicoryne brassicae) trophic levels on adalia bipunctata.three brassicaceae species, brassica napus (low glucosinolate content), brassica nigra (including sinigrin), and sinapis alba (including sinalbin) were used as host plants for two aphid species: the generalist myzus persicae and the specialist brevicoryne brassicae. each combination of aphid species and prey host plant was used to feed the polyphagous ladybird beetle, adalia bipunctata. experiments with brassicaceae species including different amounts and kinds of glucosinolates (gls) showed inc ...200114768813
olfactory responses to aphid and host plant volatile releases: (e)-beta-farnesene an effective kairomone for the predator adalia bipunctata.the volatiles released from several aphid and host plant species, alone or associated, were studied for their infochemical role in prey location. using a four-arm olfactometer, the attraction of several combinations of three aphid (myzus persicae, acyrthosiphon pisum, and brevicoryne brassicae) and three plant (vicia faba, brassica napus, and sinapis alba) species toward adalia bipunctata larvae and adults was observed. both predatory larvae and adults were attracted only by a. pisum and m. pers ...200415260221
aphid transmission of a potyvirus depends on suitability of the helper component and the n terminus of the coat protein.the present study investigates the specificity of potyviruses for aphid species. two potyviruses differing in their host range were used: zucchini yellow mosaic virus (zymv) mainly infecting cucurbits and turnip mosaic virus (tumv) mainly infecting crucifers. two sets of aphids species were used as vectors, one polyphagous (myzus persicae and aphis gossypii) and the other from crucifers (brevicoryne brassicae and lipaphis erysimi). evidence is provided that the specificity between a vector and a ...200515503223
major signaling pathways modulate arabidopsis glucosinolate accumulation and response to both phloem-feeding and chewing insects.plant responses to enemies are coordinated by several interacting signaling systems. molecular and genetic studies with mutants and exogenous signal application suggest that jasmonate (ja)-, salicylate (sa)-, and ethylene (et)-mediated pathways modulate expression of portions of the defense phenotype in arabidopsis (arabidopsis thaliana), but have not yet linked these observations directly with plant responses to insect attack. we compared the glucosinolate (gs) profiles of rosette leaves of 4-w ...200515923339
crystal structure at 1.1 angstroms resolution of an insect myrosinase from brevicoryne brassicae shows its close relationship to beta-glucosidases.the aphid brevicoryne brassicae is a specialist feeding on brassicaceae plants. the insect has an intricate defence system involving a beta-d-thioglucosidase (myrosinase) that hydrolyses glucosinolates sequestered from the host plant into volatile isothiocyanates. these isothiocyanates act synergistically with the pheromone e-beta-farnesene to form an alarm system when the aphid is predated. in order to investigate the enzymatic characteristics of the aphid myrosinase and its three-dimensional s ...200516291087
arabidopsis myrosinases tgg1 and tgg2 have redundant function in glucosinolate breakdown and insect defense.in arabidopsis and other brassicaceae, the enzyme myrosinase (beta-thioglucoside glucohydrolase, tgg) degrades glucosinolates to produce toxins that deter herbivory. a broadly applicable selection for meiotic recombination between tightly linked t-dna insertions was developed to generate arabidopsis tgg1tgg2 double mutants and study myrosinase function. glucosinolate breakdown in crushed leaves of tgg1 or tgg2 single mutants was comparable to that of wild-type, indicating redundant enzyme functi ...200616640593
predator biodiversity strengthens herbivore suppression.species diversity at lower trophic levels generally improves ecosystem functioning. however, the impact of greater predator diversity on herbivore regulation is uncertain because predator species both compete with and prey on each other. in a large-scale field experiment we examined the relationship between predator species diversity and the suppression of two herbivores, green peach and cabbage aphids, on collard plants. we show that, for both aphid species, the strength of herbivore suppressio ...200616796568
gene expression and glucosinolate accumulation in arabidopsis thaliana in response to generalist and specialist herbivores of different feeding guilds and the role of defense signaling pathways.glucosinolate accumulation and expression of glucosinolate biosynthetic genes were studied in response to four herbivores in arabidopsis thaliana (l.) wild-type (columbia) and mutant lines affected in defense signaling. herbivory on wild-type plants led to increased aliphatic glucosinolate content for three of four herbivores tested, the aphid generalist myzus persicae (sulzer), the aphid specialist brevicoryne brassicae (l.), and the lepidopteran generalist spodoptera exigua hübner. the lepidop ...200617049571
[characteristics of arthropod community in alpine cabbage fields].the study on the community structure of arthropod in the alpine cabbage fields of hubei province showed that the dominant pests were brevicoryne brassicae, mamestra brassicae and plutella xylostella, while the dominant natural enemies were diaeretiella rapae, cotesia plutella, erigonidum gramiaicolum and syrphus corollae. the richness, diversity index, evenness index and dominance index of pest and natural enemy sub-communities all changed with time. the dominance index of pest sub-community was ...200717396528
transcriptional responses of arabidopsis thaliana ecotypes with different glucosinolate profiles after attack by polyphagous myzus persicae and oligophagous brevicoryne brassicae.plants are equipped with a range of defence mechanisms against herbivorous insects. in cruciferous species, jasmonic acid, salicylic acid, and ethylene along with glucosinolates and their hydrolysis products play important roles in plant protection and plant-insect communication. in turn, a number of herbivores have adapted to plants that contain glucosinolates. as a result of adaptation to their host plants, specialized insects may elicit different plant-inducible responses than generalists. ol ...200717545220
the cabbage aphid: a walking mustard oil bomb.the cabbage aphid, brevicoryne brassicae, has developed a chemical defence system that exploits and mimics that of its host plants, involving sequestration of the major plant secondary metabolites (glucosinolates). like its host plants, the aphid produces a myrosinase (beta-thioglucoside glucohydrolase) to catalyse the hydrolysis of glucosinolates, yielding biologically active products. here, we demonstrate that aphid myrosinase expression in head/thoracic muscle starts during embryonic developm ...200717623639
biochemistry and molecular biology of arabidopsis-aphid interactions.to ensure their survival in natural habitats, plants must recognize and respond to a wide variety of insect herbivores. aphids and other hemiptera pose a particular challenge, because they cause relatively little direct tissue damage when inserting their slender stylets intercellularly to feed from the phloem sieve elements. plant responses to this unusual feeding strategy almost certainly include recognition of aphid salivary components and the induction of phloem-specific defenses. due to the ...200717691101
a novel virus isolated from the aphid brevicoryne brassicae with similarity to hymenoptera picorna-like viruses.a novel virus, brevicoryne brassicae virus (brbv), has been identified in the cabbage aphid using a method based on the random amplification of encapsidated rna. the complete sequence of the rna genome of brbv has been determined. the positive-strand genomic rna is 10 161 nt, excluding the 3' poly(a) tail, and contains a single open reading frame (positions 793-9744) encoding a putative polyprotein of 2983 aa. the n-terminal part of the polyprotein shows similarity with the structural proteins o ...200717698671
factors affecting the activity of alatae of the aphids myzus persicae (sulzer) and brevicoryne brassicae (l.). 194918116923
accumulation of glucosinolates by the cabbage aphid brevicoryne brassicae as a defense against two coccinellid species.brassica nigra plants, characterized by high levels of sinigrin, and artificial aphid diets to which sinigrin was selectively added were used to rear the crucifer specialist, brevicoryne brassicae. aphids were provided as a food source to two species of polyphagous ladybird, adalia bipunctata and coccinella septempunctata. first instar a. bipunctata were unable to survive when fed with b. brassicae reared on b. nigra or diets containing 0.2% sinigrin, but when fed with aphids reared on diets con ...200818270780
comparative transcriptome analysis of arabidopsis thaliana infested by diamond back moth (plutella xylostella) larvae reveals signatures of stress response, secondary metabolism, and signalling.plants are exposed to attack from a large variety of herbivores. feeding insects can induce substantial changes of the host plant transcriptome. arabidopsis thaliana has been established as a relevant system for the discovery of genes associated with response to herbivory, including genes for specialized (i.e. secondary) metabolism as well as genes involved in plant-insect defence signalling.200818400103
towards global understanding of plant defence against aphids--timing and dynamics of early arabidopsis defence responses to cabbage aphid (brevicoryne brassicae) attack.insect feeding on plants causes a complex series of coordinated defence responses. little is known, however, about the time-dependent aspect of induced changes. here we present a time series-based investigation of arabidopsis thaliana ler subjected to attack by a specialist pest of brassicaceae species, brevicoryne brassicae. transcriptome and metabolome changes were studied at 6, 12, 24 and 48 h after infestation to monitor the progress of early induced responses. the use of full-genome oligonu ...200818433442
[quality of different aphids as hosts of the parasitoid lysiphlebus testaceipes (cresson) (hymenoptera: braconidae, aphidiinae)].lysiphlebus testaceipes (cresson) has a broad aphid host range; however the quality of these preys may interfere in its biological feature. this study aimed to evaluate the quality of three macrosiphini, brevicoryne brassicae (l.), lipaphis erysimi (kaltenbach) and myzus persicae (sulzer), and three aphidini schizaphis graminum (rondani) rhopalosiphum maidis (fitch) and aphis gossypii glover as hosts to l. testaceipes and to determine the relation possible of host preference, of size and quality ...200818506297
responses of brassica oleracea cultivars to infestation by the aphid brevicoryne brassicae: an ecological and molecular approach.intraspecific variation in resistance or susceptibility to herbivorous insects has been widely studied through bioassays. however, few studies have combined this with a full transcriptomic analysis. here, we take such an approach to study the interaction between the aphid brevicoryne brassicae and four white cabbage (brassica oleracea var. capitata) cultivars. both under glasshouse and field conditions, two of the cultivars clearly supported a faster aphid population development than the other t ...200818721268
pathogenic fungi and parasitoids of aphids present in air captures of migratory alates in the low-latitude plateau of yunnan, china.to survey fungal pathogens and parasitoids of aphids in the low-latitude plateau of yunnan, southwest china, 3,553 migratory alates of brevicoryne brassicae, lipaphis erysimi, and myzus persicae were attracted to a yellow-plus-plant trap from air during a full-year period and individually reared on cabbage leaves for 7-14 d at 18-22 degrees c and l:d 12:12. among the trapped alates, 19.2% were killed by 10 species of aphid-pathogenic fungi after survival of 2.3 d (range, 1-7 d). another 2.8% wer ...200819036206
honeydew feeding in the solitary bee osmia bicornis as affected by aphid species and nectar availability.like honey bees (apis mellifera), non-apis bees could exploit honeydew as a carbohydrate source. in addition to providing carbohydrates, this may expose them to potentially harmful plant products secreted in honeydew. however, knowledge on honeydew feeding by solitary bees is very scarce. here we determine whether the polylectic solitary bee osmia bicornis (=o. rufa) collects honeydew under semi-field conditions, and whether this is affected by aphid species and presence of floral nectar. bees w ...200919699745
modifying the alkylglucosinolate profile in arabidopsis thaliana alters the tritrophic interaction with the herbivore brevicoryne brassicae and parasitoid diaeretiella rapae.arabidopsis thaliana was used as an experimental model plant to investigate a tritrophic interaction between the plant, a specialist aphid herbivore, brevicoryne brassicae, and its natural enemy, the parasitoid diaeretiella rapae. the a. thaliana ecotype col-5 was transformed with a functional 2-oxoglutarate dependent dioxygenase (bnigsl-alk) that converts 3-methylsulfinylpropylglucosinolate and 4-methylsulfinylbutylglucosinolate to 2-propenylglucosinolate and 3-butenylglucosinolate, respectivel ...200919701726
varying responses of insect herbivores to altered plant chemistry under organic and conventional treatments.the hypothesis that plants supplied with organic fertilizers are better defended against insect herbivores than those supplied with synthetic fertilizers was tested over two field seasons. organic and synthetic fertilizer treatments at two nitrogen concentrations were supplied to brassica plants, and their effects on the abundance of herbivore species and plant chemistry were assessed. the organic treatments also differed in fertilizer type: a green manure was used for the low-nitrogen treatment ...201019906673
behavioral evidence for local reduction of aphid-induced resistance.twenty-five aphids of three different species, brevicoryne brassicae l, myzus persicae schulzer, and rhopalosiphum padi l(hemiptera: aphididae) were each allowed to infest leaves of a young plant of their respective host plant species for 4 days, except that the oldest expanded leaf (the 'systemic' leaf) was kept free of aphids. each preinfested plant thus had two types of leaves, local leaves (preinfested with aphids) and one systemic leaf, the oldest true leaf that had been kept free of aphids ...200720345289
insecticidal activity of camptothecin against nilaparvata lugens, brevicoryne brassicae, and chilo suppressalis.greenhouse tests showed that 0.2% camptothecin emulsifiable concentrate (ec) has strong contact toxicity to three agricultural pests, with lc50 and lc90 values of 0.1-0.6 and 0.4-5.0 mg liter(-1), respectively. the descending order of susceptibility was nilaparvata lugens (ståhl) > brevicoryne brassicae (l.) > chilo suppressalis (walker). field tests showed that the corrected mortality of b. brassicae and n. lugens was 94.6 and 69.1%, respectively, which was not significantly different from that ...201020429466
turnip mosaic virus and the quest for durable resistance.summary taxonomy: turnip mosaic virus (tumv) is a member of the genus potyvirus (type species potato virus y) in the family potyviridae. to date, tumv is the only potyvirus known to infect brassicas. there are potyvirus isolates that appear serologically similar to tumv when tested with polyclonal antisera that do not readily infect brassicas (lesemann and vetten, 1985). physical properties: virions are approximately 720 x 15-20 nm flexuous rods (fig. 1) and are composed of 95% coat protein (cp) ...200220569337
temporal consistency in herbivore responses to glucosinolate polymorphism in populations of wild cabbage (brassica oleracea).natural populations of wild cabbage (brassica oleracea) show significant qualitative diversity in heritable aliphatic glucosinolates, a class of secondary metabolites involved in defence against herbivore attack. one candidate mechanism for the maintenance of this diversity is that differential responses among herbivore species result in a net fitness balance across plant chemotypes. such top-down differential selection would be promoted by consistent responses of herbivores to glucosinolates, t ...201020596728
uv-b impact on aphid performance mediated by plant quality and plant changes induced by aphids.plants face various abiotic and biotic environmental factors and therefore need to adjust their phenotypic traits on several levels. uv-b radiation is believed to impact herbivorous insects via host plant changes. plant responses to abiotic challenges (uv-b radiation) and their interaction with two aphid species were explored in a multifactor approach. broccoli plants [brassica oleracea l. convar. botrytis (l.), brassicaceae] were grown in two differently covered greenhouses, transmitting either ...201020636911
differentiation and adaptation in brassica nigra populations: interactions with related herbivores.local adaptation and population differentiation of plants are well documented, but studies on interactions with natural enemies are rare. in particular, evidence for plant adaptation to the local biotic environment, such as herbivores remains poor. we used the black mustard brassica nigra, an annual species of river valley and coastal habitats to (1) analyse population differentiation in plant traits and herbivory in a common garden experiment, (2) examine home versus away differences in a recip ...201120960010
host range limitation caused by incomplete host regulation in an aphid parasitoid.defining host ranges in parasitoid insects is important both from a theoretical and an applied point of view. based on the literature, some species seem able to use a wide range of hosts, while field studies indicate possible local host specialization. in koinobiont endoparasitoid species, such specialization could involve physiological processes. we tested the ability of two strains of the cosmopolitan and polyphagous parasitoid diaeretiella rapae, to develop in three of its recorded aphid host ...201121182844
permanent genetic resources added to molecular ecology resources database 1 august 2010-30 september 2010.this article documents the addition of 229 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: acacia auriculiformis × acacia mangium hybrid, alabama argillacea, anoplopoma fimbria, aplochiton zebra, brevicoryne brassicae, bruguiera gymnorhiza, bucorvus leadbeateri, delphacodes detecta, tumidagena minuta, dictyostelium giganteum, echinogammarus berilloni, epimedium sagittatum, fraxinus excelsior, labeo chrysophekadion, oncorhynch ...201121429127
an ecological genomic approach challenging the paradigm of differential plant responses to specialist versus generalist insect herbivores.a general prediction of the specialist/generalist paradigm indicates that plant responses to insect herbivores may depend on the degree of ecological specialization of the insect attacker. however, results from a single greenhouse experiment evaluating the responses of the model plant arabidopsis thaliana to three specialist (plutella xylostella, pieris rapae, and brevicoryne brassicae) and three generalist (trichoplusia ni, spodoptera exigua, and myzus persicae) insect species did not support t ...201121625984
testing the importance of jasmonate signalling in induction of plant defences upon cabbage aphid (brevicoryne brassicae) attack.phloem-feeding aphids deprive plants of assimilates, but mostly manage to avoid causing the mechanical tissue damage inflicted by chewing insects. nevertheless, jasmonate signalling that is induced by infestation is important in mediating resistance to phloem feeders. aphid attack induces the jasmonic acid signalling pathway, but very little is known about the specific impact jasmonates have on the expression of genes that respond to aphid attack.201121854623
Assessing aphids potato virus Y-transmission efficiency: A new approach.In order to develop an alternative method to optimize the relative efficiency factor (REF) assessment, the efficiency of transmission of Potato virus Y (PVY) by seven aphid species was examined. In vitro micropropagated potato plantlets were used to experiment on phenotypically and genetically homogeneous material. Species-specific acquisition access period (AAP) on a PVY-infected plantlet was assessed for each aphid species using electrical penetration graph (EPG) technique. Aphid probing behav ...201121884729
host quality of different aphid species for rearing diaeretiella rapae (mcintosh) (hymenoptera: braconidae).this study aimed to evaluate the quality of the aphid myzus persicae (sulzer), lipaphis erysimi (kaltenbach) and brevicoryne brassicae (l.) as hosts for the parasitoid diaeretiella rapae (mcintosh). parasitization by d. rapae was higher on m. persicae than on l. erysimi and b. brassicae. the time of development of d. rapae from egg to mummy or egg to adult male or female were shorter on m. persicae than on l. erysimi and b. brassicae. moreover, d. rapae showed no significant differences in the e ...201121952965
differences in the mechanism of inoculation between a semipersistent and a non-persistent aphid-transmitted plant virus.inoculation of the semipersistent cauliflower mosaic virus (camv, genus caulimovirus) is associated to successive brief (5-10 s) intracellular stylet punctures (pd) when aphids probe in epidermal and mesophyll cells. in contrast to non-persistent viruses, there is no evidence of which of the pd sub-phases (ii-1, ii-2 and ii-3) is involved in the inoculation of camv. experiments were conducted using the electrical penetration graph (epg) technique to investigate what particular sub-phases of the ...201122090212
plant neighborhood influences colonization of brassicaceae by specialist and generalist aphids.a plant's own characteristics, but also those of its neighbors, might have an impact on its probability of being colonized by herbivorous insects. a plant might be less colonized and experience associational resistance when it grows near repellent neighbors. in contrast, it might be more colonized and experience associational susceptibility near attractive neighbors. to date, mechanisms that drive associational defense are not really understood. in order to gain insights into the occurrence of a ...201222218942
herbivore-mediated effects of glucosinolates on different natural enemies of a specialist aphid.the cabbage aphid brevicoryne brassicae is a specialist herbivore that sequesters glucosinolates from its host plant as a defense against its predators. it is unknown to what extent parasitoids are affected by this sequestration. we investigated herbivore-mediated effects of glucosinolates on the parasitoid wasp diaeretiella rapae and the predator episyrphus balteatus. we reared b. brassicae on three ecotypes of arabidopsis thaliana that differ in glucosinolate content and on one genetically tra ...201222258357
rhizobacteria modify plant-aphid interactions: a case of induced systemic susceptibility.beneficial microbes, such as plant growth-promoting rhizobacteria and mycorrhizal fungi, may have a plant-mediated effect on insects aboveground. the plant growth-promoting rhizobacterium pseudomonas fluorescens can induce systemic resistance in arabidopsis thaliana against several microbial pathogens and chewing insects. however, the plant-mediated effect of these beneficial microbes on phloem-feeding insects is not well understood. using arabidopsis as a model, we here report that p. fluoresce ...201222348327
n'-nitro-2-hydrocarbylidenehydrazinecarboximidamides: design, synthesis, crystal structure, insecticidal activity, and structure-activity relationships.a novel series of acyclic imine-substituted nitenpyram analogues were designed and synthesized from nitroaminoguanidine, and their structures were confirmed using x-ray diffraction crystallography. preliminary bioassays showed that the target molecules exhibited good activities against aphids in laboratory (myzus persicae sulzer) and field trials (m. persicae sulzer and brevicoryne brassicae linnaeus). comparative molecular field analysis and comparative molecular similarity indices analysis wer ...201222546079
'myrosin cells' are not a prerequisite for aphid feeding on oilseed rape (brassica napus) but affect host plant preferences.the enzyme myrosinase (ec 3.2.3.1.147) is present in specialised myrosin cells and forms part of the glucosinolate-myrosinase system, also known as 'the mustard oil bomb', which has an important role in the defence system of cruciferous plants against insect pests. transgenic brassica napus mineless have been produced by transgenic ablation of myrosin cells. this prompted us to investigate the importance of myrosin cells in plant-aphid interactions. in order to study this, we challenged transgen ...201222672561
genetic engineering of plant volatile terpenoids: effects on a herbivore, a predator and a parasitoid.most insect-resistant transgenic crops employ toxins to control pests. a novel approach is to enhance the effectiveness of natural enemies by genetic engineering of the biosynthesis of volatile organic compounds (vocs). before the commercialisation of such transgenic plants can be pursued, detailed fundamental studies of their effects on herbivores and their natural enemies are necessary. the linalool/nerolidol synthase gene fanes1 was constitutively expressed from strawberry in three arabidopsi ...201322933413
plant acclimation to elevated co₂ affects important plant functional traits, and concomitantly reduces plant colonization rates by an herbivorous insect.plants growing under elevated co₂ concentration may acclimatize to this environmental change by modification of chemical, physiological, and/or morphological traits. as a consequence, not only plant functioning but also plant-insect interactions might be altered, with important consequences particularly for agricultural systems. whereas most studies have focused on the plant acclimation effects of elevated co₂ with regard to crop growth and productivity, acclimation effects on the behavioral res ...201322968910
crosstalk between above- and belowground herbivores is mediated by minute metabolic responses of the host arabidopsis thaliana.plants are frequently under attack by multiple herbivores and can be infested at their shoots as well as their roots. as a consequence, plant metabolites are readily induced, mediated by phytohormones such as salicylic acid and jasmonic acid. thereby, cross-talk between signal transduction pathways may occur if different herbivores attack the plant simultaneously. in turn, modifications in the plant metabolic pattern can affect herbivores infesting local and systemic tissue. here, an integrative ...201223045608
water stress and aphid feeding differentially influence metabolite composition in arabidopsis thaliana (l.).little is known about how drought stress influences plant secondary metabolite accumulation and how this affects plant defense against different aphids. we therefore cultivated arabidopsis thaliana (l.) plants under well-watered, drought, and water-logged conditions. two aphid species were selected for this study: the generalist myzus persicae (sulzer) and the crucifer specialist brevicoryne brassicae (l.). metabolite concentrations in the phloem sap, which influence aphid growth, changed partic ...201223144921
pest control experiments show benefits of complexity at landscape and local scales.farms benefit from pest control services provided by nature, but management of these services requires an understanding of how habitat complexity within and around the farm impacts the relationship between agricultural pests and their enemies. using cage experiments, this study measures the effect of habitat complexity across scales on pest suppression of the cabbage aphid brevicoryne brassicae in broccoli. our results reveal that proportional reduction of pest density increases with complexity ...201223210310
how conserved are the bacterial communities associated with aphids? a detailed assessment of the brevicoryne brassicae (hemiptera: aphididae) using 16s rdna.aphids harbor a community of bacteria that include obligate and facultative endosymbionts belonging to the enterobacteriaceae along with opportunistic, commensal, or pathogenic bacteria. this study represents the first detailed analysis of the identity and diversity of the bacterial community associated with the cabbage aphid, brevicoryne brassicae (l.). 16s rdna sequence analysis revealed that the community of bacteria associated with b. brassicae was diverse, with at least four different bacte ...201223321084
seasonal patterns of cold hardiness and cryoprotectant profiles in brevicoryne brassicae (hemiptera: aphididae).the cabbage aphid brevicoryne brassicae (l.) (hemiptera: aphididae), one of the most important pests of cruciferous crops, overwinters as nymph and adult on winter cabbages in tehran, iran, and forms large populations on these plants during winter. to determine the cold hardiness of this aphid, adults and first-instar nymphs were collected monthly from ornamental cabbages planted in the field from october of 2009 to may of 2010. supercooling points and lt(50) values (the temperature at which 50% ...201223321113
molecular signatures in arabidopsis thaliana in response to insect attack and bacterial infection.under the threat of global climatic change and food shortages, it is essential to take the initiative to obtain a comprehensive understanding of common and specific defence mechanisms existing in plant systems for protection against different types of biotic invaders. we have implemented an integrated approach to analyse the overall transcriptomic reprogramming and systems-level defence responses in the model plant species arabidopsis thaliana (a. thaliana henceforth) during insect brevicoryne b ...201323536844
acclimation to elevated co2 increases constitutive glucosinolate levels of brassica plants and affects the performance of specialized herbivores from contrasting feeding guilds.plants growing under elevated co2 concentration may acclimate by modifying chemical traits. most studies have focused on the effects of environmental change on plant growth and productivity. potential effects on chemical traits involved in resistance, and the consequences of such effects on plant-insect interactions, have been largely neglected. here, we evaluated the performance of two brassica specialist herbivores from contrasting feeding guilds, the leaf-feeding pieris brassicae and the phlo ...201323609163
sublethal impact of paraquat on the life span and parasitic behavior of diaeretiella rapae m'intosh.this study focuses on assessing the impact of sublethal doses of paraquat on the survival, the emergence, the life span and the parasitic behavior of diaeretiella rapae m'intosh (hymenoptera: braconidae). the impact of sublethal doses was measured at room temperature using different densities of the cabbage aphid brevicoryne brassicae. the results reveal that the field dose of paraquat caused 100% mortality in d. rapae. the percentage emergence of d. rapae decreased from 80.5% in the control gro ...201323638892
dna barcoding and elucidation of cryptic aphid species (hemiptera: aphididae) in india.rapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. in this respect, dna barcoding employing a 658 bp fragment of 5′ region of the mitochondrial cytochrome oxidase i (co-i) gene is an effective tool in addressing the above. in the present study, we employed co-i for discriminating 142 individuals representing 32 species of aphids from india. s ...201323680306
scales and drivers of local adaptation in brassica nigra (brassicaceae) populations. 201323720429
development of insecticide resistance in field populations of brevicoryne brassicae (hemiptera: aphididae) in pakistan.cabbage aphid brevicoryne brassicae (l.) (hemiptera: aphididae) is a serious pest of crucifers in pakistan. after incidences of poor control by recommended insecticides, the current study was undertaken to find out the status of insecticide resistance in pakistani b. brassicae. apterous adult aphids were bioassayed from 2006 to 2010 for their response to 12 insecticides using an adult immersion method. no or very low levels of resistance was found to endosulfan; and the organophosphates: chlorpy ...201323786087
plant-mediated interactions between shoot-feeding aphids and root-feeding nematodes depend on nitrate fertilization.the plant metabolite composition is modulated by various abiotic and biotic factors including nutrient availability and herbivory. in turn, induced changes in plant quality can affect herbivore performance and mediate indirect interactions between spatially separated herbivores sharing a host. studies on plant-mediated herbivore interactions have been carried out at single fertilization regimes only, but we hypothesized that nutrient availability modifies these interactions. therefore, we studie ...201323836091
biotic and abiotic factors affecting brevicoryne brassicae (l.) (hemiptera: aphididae) and the associated hyperparasitoid alloxysta fuscicornis hartig (hymenoptera: figitidae) morphologies.this study investigates the influence of biotic and abiotic factors associated with the morphological development of brevicoryne brassicae (linnaeus) and the associated hyperparasitoid alloxysta fuscicornis (hartig). the experiment involved the examination of whether aphid size was influenced by their vertical distribution and density on the host plant, as well as whether variations in hyperparasitoid size and symmetry were correlated with those of their aphid hosts. an aphid multivariate size i ...201223950061
influence of cornicle droplet secretions of the cabbage aphid, brevicoryne brassicae, on parasitism behavior of naïve and experienced diaeretiella rapae.insects have evolved amazing methods of defense to ward off enemies. many aphids release cornicle secretions when attacked by predators and parasitoids. these secretions contain an alarm pheromone that alerts other colony members of danger, thereby providing indirect fitness benefits to the releaser. in addition, contact with cornicle secretions could also threaten an attacker and could provide direct fitness to the releaser. however, cornicle secretions may also be recruited as a kairomonal cue ...201423956144
belowground induction by delia radicum or phytohormones affect aboveground herbivore communities on field-grown broccoli.induced plant defence in response to phytophagous insects is a well described phenomenon. however, so far little is known about the effect of induced plant responses on subsequently colonizing herbivores in the field. broccoli plants were induced in the belowground compartment using (i) infestation by the root-herbivore delia radicum, (ii) root application of jasmonic acid (ja) or (iii) root application of salicylic acid (sa). the abundance of d. radicum and six aboveground herbivores displaying ...201323970888
heterodera schachtii nematodes interfere with aphid-plant relations on brassica oleracea.aboveground and belowground herbivore species modify plant defense responses differently. simultaneous attack can lead to non-additive effects on primary and secondary metabolite composition in roots and shoots. we previously found that aphid (brevicoryne brassicae) population growth on brassica oleracea was reduced on plants that were infested with nematodes (heterodera schachtii) prior (4 weeks) to aphid infestation. here, we examined how infection with root-feeding nematodes affected primary ...201324014097
intra-specific variation in wild brassica oleracea for aphid-induced plant responses and consequences for caterpillar-parasitoid interactions.herbivore-induced plant responses not only influence the initiating attackers, but also other herbivores feeding on the same host plant simultaneously or at a different time. insects belonging to different feeding guilds are known to induce different responses in the host plant. changes in a plant's phenotype not only affect its interactions with herbivores but also with organisms higher in the food chain. previous work has shown that feeding by a phloem-feeding aphid on a cabbage cultivar facil ...201424178834
tri-trophic insecticidal effects of african plants against cabbage pests.botanical insecticides are increasingly attracting research attention as they offer novel modes of action that may provide effective control of pests that have already developed resistance to conventional insecticides. they potentially offer cost-effective pest control to smallholder farmers in developing countries if highly active extracts can be prepared simply from readily available plants. field cage and open field experiments were conducted to evaluate the insecticidal potential of nine com ...201324205287
olfactory responses of the parasitoiddiaeretiella rapae (hymenoptera: aphidiidae) to odor of plants, aphids, and plant-aphid complexes.diaeretiella rapae (m'intosh) (hymenoptera: aphidiidae) is a parasitoid of several aphid species, including the russian wheat aphid (rwa),diuraphis noxia (mordvilko), and the cabbage aphid (ca).brevicoryne brassicae (l.). the response of matedd. rapae females to odors from wheat, cabbage, and plant-host complexes was investigated using a four-choice olfactometer. experienced parasitoids, but not inexperienced females, responded positively to odors of the wheat-rwa complex in a no-choice test. in ...199524234172
testing local host adaptation and phenotypic plasticity in a herbivore when alternative related host plants occur sympatrically.host race formation in phytophagous insects can be an early stage of adaptive speciation. however, the evolution of phenotypic plasticity in host use is another possible outcome. using a reciprocal transplant experiment we tested the hypothesis of local adaptation in the aphid brevicoryne brassicae. aphid genotypes derived from two sympatric host plants, brassica oleracea and b. campestris, were assessed in order to measure the extent of phenotypic plasticity in morphological and life history tr ...201324265743
un-nesting dna russian dolls - the potential for constructing food webs using residual dna in empty aphid mummies.constructing food-web assemblages comprising parasitoid wasps involves large field collections of hosts followed by labour-intensive rearing of the insects to evaluate the rates of parasitism along with morphological or molecular identification of the parasitoid species. this article presents research towards a new molecular method for the practical and accurate construction of aphid-based food webs. we hypothesize that parasitoid and hyperparasitoid dna left inside aphid mummies after emergence ...201424341932
bottom-up and top-down herbivore regulation mediated by glucosinolates in brassica oleracea var. acephala.quantitative differences in plant defence metabolites, such as glucosinolates, may directly affect herbivore preference and performance, and indirectly affect natural enemy pressure. by assessing insect abundance and leaf damage rate, we studied the responses of insect herbivores to six genotypes of brassica oleracea var. acephala, selected from the same cultivar for having high or low foliar content of sinigrin, glucoiberin and glucobrassicin. we also investigated whether the natural parasitism ...201424352843
brassica plant responses to mild herbivore stress elicited by two specialist insects from different feeding guilds.compensation growth and chemical defense are two components of plant defense strategy against herbivores. in this study, compensation growth and the response of primary and secondary metabolites were investigated in brassica rapa plants subjected to infestation by two herbivores from contrasting feeding guilds, the phloem-feeding aphid brevicoryne brassicae and the leaf-feeding caterpillar pieris brassicae. these specialist herbivores were used at two different densities and allowed to feed for ...201424500734
modulation of flavonoid metabolites in arabidopsis thaliana through overexpression of the myb75 transcription factor: role of kaempferol-3,7-dirhamnoside in resistance to the specialist insect herbivore pieris brassicae.anthocyanins and flavonols are secondary metabolites that can function in plant defence against herbivores. in arabidopsis thaliana, anthocyanin and flavonol biosynthesis are regulated by myb transcription factors. overexpression of myb75 (oxmyb75) in arabidopsis results in increasing anthocyanin and flavonol levels which enhances plant resistance to generalist caterpillars. however, how these metabolites affect specialist herbivores has remained unknown. performance of a specialist aphid (brevi ...201424619996
caterpillar-induced plant volatiles remain a reliable signal for foraging wasps during dual attack with a plant pathogen or non-host insect herbivore.plants respond to herbivory with the emission of plant volatiles, which can be used by the herbivores' natural enemies to locate their hosts or prey. in nature, plants are often simultaneously confronted with insect herbivores and phytopathogens, potentially interfering with the attraction of the herbivores' enemies as a result of modifications of the induced volatile blend. here, we investigated parasitoid (cotesia glomerata) attraction to volatiles of plants challenged by different attackers, ...201424697624
constitutive overexpression of the pollen specific gene sks13 in leaves reduces aphid performance on arabidopsis thaliana.plants have developed a variety of mechanisms to counteract aphid attacks. they activate their defences by changing the expression of specific genes. previously we identified an activation tag mutant of arabidopsis thaliana on which myzus persicae population development was reduced. activation tag mutants are gain-of-function in which the expression of a gene is increased by the insertion of the cauliflower mosaic virus 35s enhancer that acts on the natural promoter. by further characterizing th ...201425267093
density-dependent interference of aphids with caterpillar-induced defenses in arabidopsis: involvement of phytohormones and transcription factors.in nature, plants are exposed to attacks by multiple herbivore species at the same time. to cope with these attacks, plants regulate defenses with the production of hormones such as salicylic acid (sa) and jasmonic acid (ja). because herbivore densities are dynamic in time, this may affect plant-mediated interactions between different herbivores attacking at the same time. in arabidopsis thaliana, feeding by brevicoryne brassicae aphids interferes with induced defenses against plutella xylostell ...201525339349
qualitative and quantitative prey requirements of two aphidophagous coccinellids, adalia tetraspilota and hippodamia variegata.the suitability of two prey species, aphis pomi de geer (hemiptera: aphididae) and brevicoryne brassicae (l.), for two generalist aphidophagous coccinellids, adalia tetraspilota (hope) (coleoptera: coccinellidae) and hippodamia variegata (goeze), at various abundance levels was investigated under laboratory conditions. while both a. pomi and b. brassicae were found to be suitable, the predators performed better when feeding upon b. brassicae. the prey densities affected the developmental paramet ...201425373219
transcriptional responses of arabidopsis thaliana to chewing and sucking insect herbivores.we tested the hypothesis that arabidopsis can recognize and respond differentially to insect species at the transcriptional level using a genome wide microarray. transcriptional reprogramming was characterized using co-expression analysis in damaged and undamaged leaves at two times in response to mechanical wounding and four insect species. in all, 2778 (10.6%) of annotated genes on the array were differentially expressed in at least one treatment. responses differed mainly between aphid and ca ...201425452759
parasitoid- and hyperparasitoid-mediated seasonal dynamics of the cabbage aphid (hemiptera: aphididae).the population dynamics of the cabbage aphid, brevicoryne brassicae (l.), its parasitoid, diaeretiella rapae mcintosh, and hyperparasitoids, pachyneuron spp., were quantified under field conditions during 2011-2013, by examining synchronization, parasitoid: aphid ratio, possible effect of density on the finite rate of increase, and spatial coincidence. the rates of parasitism and hyperparasitism were based on rearing field-collected mummies and live parasitized aphids, and density of the aphid w ...201425479198
local and systemic transcriptional responses to crosstalk between above- and belowground herbivores in arabidopsis thaliana.plants are often simultaneously infested by several herbivores at the shoots and roots. recent results revealed that the model plant arabidopsis thaliana shows highly challenge-specific local and systemic responses to individual and simultaneous attacks of shoot-infesting aphids and root-infesting nematodes at the metabolome level. (1) here, we present the corresponding transcriptional changes in plants treated with brevicoryne brassicae aphids and heterodera schachtii nematodes individually and ...201425482783
biology and life table parameters of brevicoryne brassicae (hemiptera: aphididae) on cauliflower cultivars.in this article, the biology and fertility life table parameters of the cabbage aphid, brevicoryne brassicae (l.) (hemiptera: aphididae), were studied on cauliflower leaves, brassica oleracea var. botrytis (brassicales: brassicaceae), of the cultivars smilla, snow mystique, white cloud, buris, galiblanka, snow crown, sg, and tokita. this study was conducted under controlled conditions: 25 ± 2°c, 65 ± 5% relative humidity (rh), and 16:8 (l:d) h photoperiods. statistical analysis showed that there ...201425527591
systematic analysis of phloem-feeding insect-induced transcriptional reprogramming in arabidopsis highlights common features and reveals distinct responses to specialist and generalist insects.phloem-feeding insects (pfis), of which aphids are the largest group, are major agricultural pests causing extensive damage to crop plants. in contrast to chewing insects, the nature of the plant response to pfis remains poorly characterized. scrutiny of the literature concerning transcriptional responses of model and crop plant species to pfis reveals surprisingly little consensus with respect to the transcripts showing altered abundance following infestation. nevertheless, core features of the ...201525540442
the diversion of 2-c-methyl-d-erythritol-2,4-cyclodiphosphate from the 2-c-methyl-d-erythritol 4-phosphate pathway to hemiterpene glycosides mediates stress responses in arabidopsis thaliana.2-c-methyl-d-erythritol-2,4-cyclodiphosphate (mecdp) is an intermediate of the plastid-localized 2-c-methyl-d-erythritol-4-phosphate (mep) pathway which supplies isoprenoid precursors for photosynthetic pigments, redox co-factor side chains, plant volatiles, and phytohormones. the arabidopsis hds-3 mutant, defective in the 1-hydroxy-2-methyl-2-(e)-butenyl-4-diphosphate synthase step of the mep pathway, accumulates its substrate mecdp as well as the free tetraol 2-c-methyl-d-erythritol (me) and g ...201525704332
attraction of egg-killing parasitoids toward induced plant volatiles in a multi-herbivore context.in response to insect herbivory, plants emit volatile organic compounds which may act as indirect plant defenses by attracting natural enemies of the attacking herbivore. in nature, plants are often attacked by multiple herbivores, but the majority of studies which have investigated indirect plant defenses to date have focused on the recruitment of different parasitoid species in a single-herbivore context. here, we report our investigation on the attraction of egg parasitoids of lepidopteran ho ...201525953114
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