Publications

construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.	a mutant of moloney murine leukemia virus (m-mulv), pmov-psi-, was constructed by deletion of about 350 nucleotides from an infectious proviral dna clone between the putative env mrna 5' splice site and the aug that initiates the coding sequence for pr65gag. although the parent wild-type proviral clone, pmov-psi+, quickly causes a high level of reverse-transcriptase-containing virus particles to be released from transfected nih/3t3 cells, transfection of pmov-psi- into these cells initially resu ...	1983	6678608
complete sequence of the rna 1 and rna 2 of parietaria mottle virus.		2006	16773234
complete nucleotide sequence of a spanish isolate of parietaria mottle virus infecting tomato.	the genome of a spanish isolate of parietaria mottle virus (pmov) obtained from tomato (strain pmov-t) was completely sequenced. protein motifs conserved for rna viruses were identified: the p1 protein contained a metyltransferase domain in its n-terminal half and a triphosphatase/ helicase domain in its c-terminal half, the p2 protein contained a rna polymerase domain; the 3a protein contained a rna-binding domain with α-helix and β-sheet secondary structures. in addition, stem-loop structures ...	2009	19636693
genomic sequences of blackberry chlorotic ringspot virus and strawberry necrotic shock virus and the phylogeny of viruses in subgroup 1 of the genus ilarvirus.	three members of subgroup 1 of the genus ilarvirus: blackberry chlorotic ringspot (bcrv), strawberry necrotic shock (snsv), and tobacco streak viruses (tsv), may infect rubus and fragaria species. all cause symptoms similar to those previously attributed to infection by tsv alone. although similarities exist among the genomic sequences of the three, phylogenetic analysis shows them to be distinct viruses. these viruses and parietaria mottle virus, the other currently accepted member of subgroup ...	2010	20195656
two basic (hydrophilic) regions in the movement protein of parietaria mottle virus have rna binding activity and are required for cell-to-cell transport.	the movement protein (mp) of parietaria mottle virus (pmov) is required for virus cell-to-cell movement. bioinformatics analysis identified two hydrophilic non-contiguous regions (r1 and r2) rich in the basic amino acids lysine and arginine and with the predicted secondary structure of an α-helix. different approaches were used to determine the implication of the r1 and r2 regions in rna binding, plasmodesmata (pd) targeting and cell-to-cell movement. emsa (electrophoretic mobility shift assay) ...	2014	24583367
genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange.	the genetic variability and evolution of parietaria mottle virus (pmov) of the genus ilarvirus was studied by analyzing nucleotide sequences of 2b and cp genes from isolates collected in different countries. phylogenetic analysis showed that pmov isolates clustered in different clades: one (clade i) composed of only italian isolates and three clades (clades ii-iv) including the spanish isolates. the greek isolate grt-1 used in this study was in clade iv for the cp phylogenetic tree whereas it fo ...	2015	26234185
complete genome sequence of a tomato isolate of parietaria mottle virus from italy.	we report here the complete genome sequence of isolate t32 of parietaria mottle virus (pmov) infecting tomato plants in turin, italy, obtained by sanger sequencing. t32 shares 90.48 to 96.69% nucleotide identity with other two pomv isolates, cr8 and pe1, respectively, whose complete genome sequences are available.	2015	26679580