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s-related protein can be recombined with self-compatibility in interspecific derivatives of lycopersicon.stylar proteins involved in the self-incompatible (si) response of lycopersicon hirsutum have been identified and mapped to the locus that controls si (s locus). l. esculentum, a self-compatible (sc) species of cultivated tomato, does not display these proteins. hybrids between sc l. esculentum and si l. hirsutum are self-sterile despite these individuals bearing pollen containing the s allele of l. esculentum. in progeny derived from backcrossing the hybrids to l. esculentum, there was a strong ...19958595049
identification and ds-tagged isolation of a new gene at the cf-4 locus of tomato involved in disease resistance to cladosporium fulvum race 5.leaf mould disease in tomato is caused by the biotrophic fungus cladosporium fulvum. an ac/ds targeted transposon tagging strategy was used to isolate the gene conferring resistance to race 5 of c. fulvum, a strain expressing the avirulence gene avr4. an infection assay of 2-week-old seedlings yielded five susceptible mutants, of which two had a ds element integrated in the same gene at different positions. this gene, member of a gene family, showed high sequence homology to the c. fulvum resist ...19989670557
development of a set of near isogenic and backcross recombinant inbred lines containing most of the lycopersicon hirsutum genome in a l. esculentum genetic background: a tool for gene mapping and gene discovery.a novel population consisted of a set of 99 near isogenic lines (nils) and backcross recombinant inbred lines (bcrils) derived from a cross between the cultivated tomato lycopersicon esculentum cv. e6206 and l. hirsutum accession la1777 is presented. most of the lines contain a single defined introgression from l. hirsutum in the l. esculentum genetic background and together, the lines provide a coverage of more than the 85% of the l. hirsutum genome. these lines represent a new tool to uncover ...200011081970
ancient origin of pathogen recognition specificity conferred by the tomato disease resistance gene pto.we have investigated the origin of the pto disease resistance (r) gene that was previously identified in the wild tomato species lycopersicon pimpinellifolium and isolated by map-based cloning. pto encodes a serine-threonine protein kinase that specifically recognizes strains of pseudomonas syringae pv. tomato (pst) that express the avirulence gene avrpto. we examined an accession of the distantly related wild species lycopersicon hirsutum var. glabratum that exhibits avrpto-specific resistance ...200111172075
characterization and mapping of resistance to oidium lycopersicum in two lycopersicon hirsutum accessions: evidence for close linkage of two ol-genes on chromosome 6 of tomato.the cultivated tomato is susceptible to powdery mildew (oidium lycopersicum). several accessions of wild species are resistant. in this study we describe (i) the genetics and mapping of resistance to o. lycopersicum in g1.1290, one of the resistant accessions in lycopersicon hirsutum, (ii) fine mapping of ol-1 originated from l. hirsutum g1.1560, another resistant accession of l. hirsutum, and (iii) tests of allelism for resistance in g1.1290 and g1.1560. initially, it is demonstrated that the r ...200011240617
evidence for involvement of phytochrome in tumor development on plants.the regulation of nonpathogenic tumorous growths on tomato plants by red and far-red radiation was studied using leaf discs floated on water and irradiated from beneath. it was found that red light (600-700 nanometers) was required for the induction of tumors on tomato (lycopersicon hirsutum humb. & bonpl. plant introduction la 1625), while both blue (400-500 nanometers) and green (500-600 nanometers) light had little effect on tumor development. detailed studies with red light demonstrated that ...198811537433
sesquiterpene carboxylic acids from a wild tomato species affect larval feeding behavior and survival of helicoverpa zea and spodoptera exigua (lepidoptera: noctuidae).the sesquiterpene carboxylic acids (sca), (+)-(e)-alpha-santalen-12-oic, (-)-(e)-endo-alpha-bergamoten-12-oic, and (+)-(e)-endo-beta-bergamoten-12-oic acid, are produced in glandular trichomes of lycopersicon hirsutum f. typicum humb. & bonpl. accession (la) 1777, which is highly resistant to a range of pests of cultivated tomatoes. l. esculentum mill. exposure of the larvae of two key tomato pests, tomato fruitworm [helicoverpa zea (boddie)] and beet armyworm [spodoptera exigua (hübner)], to th ...200111681691
production and quantification of methyl ketones in wild tomato accessions.production of methyl ketones as naturally occurring insecticides from wild tomato accessions is explored in this study. density of two glandular trichomes (type iv and type vi) on the leaves of six wild tomato accessions of lycopersicon hirsutum f. glabratum (mull); three accessions of l. hirsutum f. typicum (humb & bonpl.); two accessions of l. pennellii corr. (d'arcy); and density of type vi glandular trichomes of the commercial tomato lycopersicon esculentum cv. fabulous are reported. densiti ...200111757742
a molecular linkage map of tomato displaying chromosomal locations of resistance gene analogs based on a lycopersicon esculentum x lycopersicon hirsutum cross.a molecular linkage map of tomato was constructed based on a bc1 population (n = 145) of a cross between lycopersicon esculentum mill. line nc84173 (maternal and recurrent parent) and lycopersicon hirsutum humb. and bonpl. accession pi126445. nc84173 is an advanced breeding line that is resistant to several tomato diseases, not including early blight (eb) and late blight (lb). pi126445 is a self-incompatible accession that is resistant to many tomato diseases, including eb and lb. the map includ ...200211908656
sucrose uptake, invertase localization and gene expression in developing fruit of lycopersicon esculentum and the sucrose-accumulating lycopersicon hirsutum.by using immunolocalization and differential extraction methods we show that only apoplastic invertase, but not vacuolar invertase, was present in the mature, sucrose-accumulating l. hirsutum pericarp. in contrast, in the hexose-accumulating l. esculentum fruit, both the apoplastic and vacuolar invertase activities and protein content increase in the mature fruit. quantitative expression studies of the soluble invertase gene (tiv1) and the apoplastic invertase genes (lins) showed that only tiv1 ...200212010465
identification of qtls for early blight ( alternaria solani) resistance in tomato using backcross populations of a lycopersicon esculentum x l. hirsutum cross.most commercial cultivars of tomato, lycopersicon esculentum mill., are susceptible to early blight (eb), a devastating fungal ( alternaria solani sorauer) disease of tomato in the northern and eastern parts of the u.s. and elsewhere in the world. the disease causes plant defoliation, which reduces yield and fruit quality, and contributes to significant crop loss. sources of resistance have been identified within related wild species of tomato. the purpose of this study was to identify and valid ...200212582599
recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato ( lycopersicon spp.) and pepper ( capsicum spp.) genomes.resistance against both potato virus y (pvy) and tobacco etch virus (tev) was identified in the wild tomato relative lycopersicon hirsutum pi247087. analysis of the segregation ratio in f(2)/f(3) and bc(1) interspecific progenies indicated that a single recessive gene, or two very tightly linked recessive loci, are involved in resistance to both potyviruses. this locus was named pot-1. using amplified fragment length polymorphism markers and a set of l. hirsutum introgression lines, pot-1 was ma ...200212582910
zingiberene and curcumene in wild tomato.composition of ginger oil prepared from fresh ginger rhizomes, zingiber officinale roscoe (zingiberaceae) was determined by gas chromatography (gc) and gc-mass spectrometric techniques. the main sesquiterpene hydrocarbons identified were alpha-zingiberene (27-30%), alpha-curcumene (8-9%), beta-sesquiphellandrene (4.8%). and bisabolene (3.2%). the function of zingiberene and curcumene as insecticides, repellents, and insect feeding deterrents has been previously reported. other plant species havi ...200312856930
complex unilateral hybridization in lycopersicon hirsutum. 196113767338
towards the introducing of resistance to powdery mildew from lycopersicon hirsutum into l. esculentum.genes of resistance to oidium lycopersicum from lycopersicon hirsutum la 1775 were introduced to l. esculentum. breeding procedures were based on a one-way programme up to the f2 generation and then four different methods were adopted to obtain f4 and bc4 populations. screening tests among those hybrid populations were performed in a greenhouse and showed segregation for resistance to powdery mildew due to different genetic backgrounds of the families derived from four breeding methods that chan ...200114564061
mutations in potato virus y genome-linked protein determine virulence toward recessive resistances in capsicum annuum and lycopersicon hirsutum.the recessive resistance genes pot-1 and pvr2 in lycopersicon hirsutum and capsicum annuum, respectively, control potato virus y (pvy) accumulation in the inoculated leaves. infectious cdna molecules from two pvy isolates differing in their virulence toward these resistances were obtained using two different strategies. chimeras constructed with these cdna clones showed that a single nucleotide change corresponding to an amino acid substitution (arg119his) in the central part of the viral protei ...200415000399
mapping, genetic effects, and epistatic interaction of two bacterial canker resistance qtls from lycopersicon hirsutum.two quantitative trait loci (qtl) from lycopersicon hirsutum, rcm 2.0 and rcm 5.1, control resistance to clavibacter michiganensis subsp. michiganensis ( cmm). to precisely map both loci, we applied interval mapping techniques to 1,056 individuals in three populations exhibiting f(2) segregation. based on a 1-lod confidence interval, rcm 2.0 mapped to a 14.9-cm interval on chromosome 2 and accounted for 25.7-34.0% of the phenotypic variation in disease severity. rcm 5.1 mapped to a 4.3-cm interv ...200415067391
qtl analysis of quantitative resistance to phytophthora infestans (late blight) in tomato and comparisons with potato.quantitative trait loci (qtls) for resistance to phytophthora infestans (late blight) were mapped in tomato. reciprocal backcross populations derived from cultivated lycopersicon esculentum x wild lycopersicon hirsutum (bc-e, backcross to l. esculentum; bc-h, backcross to l. hirsutum) were phenotyped in three types of replicated disease assays (detached-leaflet, whole-plant, and field). linkage maps were constructed for each bc population with rflps. resistance qtls were identified on all 12 tom ...200415190365
proteomic analysis of resistance mediated by rcm 2.0 and rcm 5.1, two loci controlling resistance to bacterial canker of tomato.two quantitative trait loci from lycopersicon hirsutum, rcm 2.0 and rcm 5.1, control resistance to clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato. lines containing rcm 2.0 and rcm 5.1 and a susceptible control line were compared at 72 and 144 h postinoculation, using 2-dimensional gel electrophoresis to identify proteins regulated in response to c. michiganensis subsp. michiganensis infection. a total of 47 proteins were subjected to tandem mass sp ...200415384492
genetics of hybrid incompatibility between lycopersicon esculentum and l. hirsutum.we examined the genetics of hybrid incompatibility between two closely related diploid hermaphroditic plant species. using a set of near-isogenic lines (nils) representing 85% of the genome of the wild species lycopersicon hirsutum (solanum habrochaites) in the genetic background of the cultivated tomato l. esculentum (s. lycopersicum), we found that hybrid pollen and seed infertility are each based on 5-11 qtl that individually reduce hybrid fitness by 36-90%. seed infertility qtl act additivel ...200515466436
metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones.medium-length methylketones (c7-c15) are highly effective in protecting plants from numerous pests. we used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato lycopersicon hirsutum f glabratum (accession pi126449). a comparison of gland est databases from accession pi126449 and a second l. hirsutum accession, la1777, whose glands do not contain methylketones, showed that the expression of genes for fatty ac ...200515772286
occurrence of colombian datura virus in brugmansia hybrids, physalis peruviana l. and solanum muricatum ait. in hungary.colombian datura virus (cdv) has been found to infect angel trumpets (brugmansia spp.) frequently and cape gooseberry (physalis peruviana) and pepino (solanum muricatum) sporadically in hungary. a cdv brg/h isolate was characterized. it had flexuous thread-like virions of about 750 x 12 nm in size. host range and symptomathological studies revealed its great similarity to authentic cdv isolates. nicotiana tabacum cultivars and lines resistant to potato virus y (pvyn) either genically or transgen ...200516047739
a major qtl introgressed from wild lycopersicon hirsutum confers chilling tolerance to cultivated tomato (lycopersicon esculentum).many plants of tropical or subtropical origin, such as tomato, suffer damage under chilling temperatures (under 10 degrees c but above 0 degrees c). an earlier study identified several quantitative trait loci (qtls) for shoot turgor maintenance (stm) under root chilling in an interspecific backcross population derived from crossing chilling-susceptible cultivated tomato (lycopersicon esculentum) and chilling-tolerant wild l. hirsutum. the qtl with the greatest phenotypic effect on stm was locate ...200516075210
the cf-4 and cf-9 resistance genes against cladosporium fulvum are conserved in wild tomato species.the cf-4 and cf-9 genes originate from the wild tomato species lycopersicon hirsutum and l. pimpinellifolium and confer resistance to strains of the leaf mold fungus cladosporium fulvum that secrete the avr4 and avr9 elicitor proteins, respectively. homologs of cf-4 and cf-9 (hcr9s) are located in several clusters and evolve mainly through sequence exchange between homologs. to study the evolution of cf genes, we set out to identify functional hcr9s that mediate recognition of avr4 and avr9 (des ...200516167771
genetic transfer of a twospotted spider mite (acari: tetranychidae) repellent in tomato hybrids.lycopersicon hirsutum dunal is very resistant to arthropod herbivory, and research on causes of resistance has often implicated trichomes and their secretions. to better understand relationships among resistance, repellency, and 2,3-dihydrofarnesoic acid, a trichome-borne sesquiterpenoid spider mite repellent, two tomato, lycopersicon esculentum miller, varieties were interbred with a highly resistant, spider mite repellent accession (la1363) of l. hirsutum. backcross and f2 generations were pro ...200516334344
natural products: repellency and toxicity of wild tomato leaf extracts to the two-spotted spider mite, tetranychus urticae koch.the potential of using phytochemicals from leaves of wild tomato for controlling the two-spotted spider mite, tetranychus urticae koch, is explored in this study as a promising alternative to the use of synthetic pesticides. wild tomato accessions of lycopersicon hirsutum plants that are not consumed by humans were planted under greenhouse conditions for mass production of leaves. crude extracts from leaves of three accessions of l. hirsutum, six accessions of l. hirsutum f. glabratum, and one a ...200616393894
temperature and photoperiod influence trichome density and sesquiterpene content of lycopersicon hirsutum f. hirsutum.resistance to colorado potato beetle in a clone of lycopersicon hirsutum f. hirsutum l. is attributed to the presence of the sesquiterpene zingiberene in the type vi leaf trichomes; however, both day/night temperature regimen and photoperiod affect zingiberene content and trichome density. in short days (sd), zingiberene content per trichome is more than 3-fold greater than in long days. in sd, trichome density per unit leaf surface is 2-fold greater at 25/20 degrees c (day/night) than at either ...199216653137
assay of chilling injury in wild and domestic tomatoes based on photosystem activity of the chilled leaves.tomato leaves were detached and stored at 0 c for various periods of time. chloroplasts were isolated from the leaves and their photoreductive activities were determined. comparisons were made between two altitudinal forms of the wild tomato lycopersicon hirsutum humb. and bonpl. (a tropical lowlands form and a highlands form adapted to growth at 3,100 meters), and two cultivars of the domestic tomato l. esculentum mill. in each case the capacity of the isolated chloroplasts to photoreduce ferri ...197916660815
temperature-induced leakage from chilling-sensitive and chilling-resistant plants.leakage rates were determined from leaf cells loaded with rubidium and [(3)h]leucine. there was a differential response between leucine and rubidium leakage depending upon the species used. the rate of leucine leakage shows a small decline below 5 c for two altitudinal variants of lycopersicon hirsutum humb. and bonpl., whereas lycopersicon esculentum l. showed a marked increase below 5 c. rubidium showed a marked increase in leakage rate below 10 c with the altitudinal variants, with only a sli ...198116661859
ripening behavior of wild tomato species.nine wild tomato species were surveyed for variability in ripening characteristics. external signs of ripening, age of fruit at ripening, and ethylene production patterns were compared. ethylene production was monitored using an ethylene-free air stream system and gas chromatography. based on these ripening characteristics, the fruits fell into three general categories: those that change color when they ripen, green-fruited species that abscise prior to ripening, and green-fruited species that r ...198116662121
physical properties of mitochondrial lipids from lycopersicon hirsutum.mitochondrial lipids from lycopersicon hirsutum undergo a broad thermal transition beginning well below 0 degrees c and ending at approximately 25 degrees c. differential thermal analysis of mitochondrial lipids isolated from ecotypes of l. hirsutum that differ in chilling sensitivity indicates that these lipid preparations have physically similar properties. this was confirmed by electron-spin-resonance experiments, although this technique failed to detect the broad transition detected by diffe ...198216662499
sensitivity of altitudinal ecotypes of the wild tomato lycopersicon hirsutum to chilling injury.the transition temperature of the leaf polar lipids and the critical temperature for chill-induced inhibition of photosynthesis was determined for three altitudinal ecotypes of the wild tomato lycopersicon hirsutum. photosynthesis was measured as co(2)-dependent o(2) evolution at 25 degrees c after leaf slices were exposed to chilling temperatures for 2 hours at a moderate photon flux density of 450 micromoles per square meter per second. the transition temperature of the leaf polar lipids was d ...198916667203
sucrose phosphate synthase, sucrose synthase, and invertase activities in developing fruit of lycopersicon esculentum mill. and the sucrose accumulating lycopersicon hirsutum humb. and bonpl.the green-fruited lycopersicon hirsutum humb. and bonpl. accumulated sucrose to concentrations of about 118 micromoles per gram fresh weight during the final stages of development. in comparison, lycopersicon esculentum mill. cultivars contained less than 15 micromoles per gram fresh weight of sucrose at the ripe stage. glucose and fructose levels remained relatively constant throughout development in l. hirsutum at 22 to 50 micromoles per gram fresh weight each. starch content was low even at e ...199116668028
temporally extended gene expression of the adp-glc pyrophosphorylase large subunit (agpl1) leads to increased enzyme activity in developing tomato fruit.tomato plants (solanum lycopersicum) harboring the allele for the agpase large subunit (agpl1) derived from the wild species solanum habrochaites (agpl1 ( h )) are characterized by higher agpase activity and increased starch content in the immature fruit, as well as higher soluble solids in the mature fruit following the breakdown of the transient starch, as compared to fruits from plants harboring the cultivated tomato allele (agpl1 ( e )). comparisons of agpase subunit gene expression and prot ...200616770584
comparative genetics of potential prezygotic and postzygotic isolating barriers in a lycopersicon species cross.i compare the genetic basis of quantitative traits that potentially contribute to pre- and postzygotic isolation between the plant species solanum lycopersicum (formerly lycopersicon esculentum) and solanum habrochaites (formerly lycopersicon hirsutum), using quantitative trait loci (qtl) mapping in a set of near-isogenic lines. putative prezygotic isolating traits include flower size, flower shape, stigma exertion, and inflorescence length, that can influence pollinator preferences and/or selfi ...200717229779
haploid selection for low temperature tolerance of tomato pollen.pollen grains were harvested from an interspecific f(1) hybrid between the cultivated tomato, lycopersicon esculentum mill., and its wild relative lycopersicon hirsutum humb. & bonpl., a low temperature tolerant accession originating from an altitude of 3200 m in the peruvian andes. the two species differ for electrophoretically-detectable loci that mark six (possibly seven) of the 12 tomato chromosomes. isozyme analysis of the bc(1) populations derived from controlled pollinations at normal and ...198217246078
the inheritance of unilateral incompatibility in lycopersicon hirsutum. 196417248219
the construction of a solanum habrochaites lyc4 introgression line population and the identification of qtls for resistance to botrytis cinerea.tomato (solanum lycopersicum) is susceptible to grey mold (botrytis cinerea). partial resistance to this fungus has been identified in accessions of wild relatives of tomato such as solanum habrochaites lyc4. in a previous f(2) mapping study, three qtls conferring resistance to b. cinerea (rbcq1, rbcq2 and rbcq4a) were identified. as it was probable that this study had not identified all qtls involved in resistance we developed an introgression line (il) population (n = 30), each containing a s. ...200717273845
candidate genes and quantitative trait loci affecting fruit ascorbic acid content in three tomato populations.fresh fruit and vegetables are a major source of ascorbic acid (vitamin c), an important antioxidant for the human diet and also for plants. ascorbic acid content in fruit exhibits a quantitative inheritance. quantitative trait loci (qtl) for ascorbic acid content have been mapped in three tomato populations derived from crosses between cultivated tomato varieties (solanum lycopersicum accessions) and three related wild species or subspecies. the first population consists of a set of introgressi ...200717277090
2-tridecanone: a naturally occurring insecticide from the wild tomato lycopersicon hirsutum f.glabratum.a nonalkaloid insecticide was isolated from the wild tomato lycopersicon hirsutum f. glabratum and identified as 2-tridecanone, a compound 72 times more abundant in the wild tomato than in the cultivated tomato l. esculentum. lepidopterous larvae (manduca sexta and heliothis zea) and aphids (aphis gossypii) died when confined on 2-tridecanone-treated filter paper.198017729870
the identification of a gene (cwp1), silenced during solanum evolution, which causes cuticle microfissuring and dehydration when expressed in tomato fruit.one of the most intriguing phenomena of fleshy fruit is the ability to maintain high water content at maturity, even following harvest. this is accomplished by a fruit cuticle that is highly impermeable to water diffusion. in this paper, we report on a novel genotype of tomato, developed via introgression from the wild species solanum habrochaites, which is characterized by microfissuring of the fruit cuticle and dehydration of the mature fruit. the microfissure/dehydration phenotype is inherite ...200717877702
response of tomato and its wild relatives in the genus solanum to cucumber mosaic virus and satellite rna combinations.the differential response of 29 genotypes of tomato and wild tomato relatives (solanum section lycopersicon species) to cucumber mosaic virus strain fny (cmv-fny), alone or in combination with three different satellite rna (satrna) variants, allowed the identification of four disease phenotype patterns, each including plants that developed very severe symptoms (leaf malformations, top stunting and lethal necrosis) and plants that remained asymptomatic. no resistance or tolerance to cmv-fny was o ...200717947544
expression of stress-response proteins upon whitefly-mediated inoculation of tomato yellow leaf curl virus in susceptible and resistant tomato plants.to better understand the nature of resistance of tomato to the whitefly (bemisia tabaci, b biotype)-transmitted tomato yellow leaf curl virus (tylcv), whiteflies and tylcv were considered as particular cases of biotic stresses and virus resistance as a particular case of successful response to these stresses. two inbred tomato lines issued from the same breeding program that used solanum habrochaites as a tylcv resistance source, one susceptible and the other resistant, were used to compare the ...200717977149
mapping and characterization of novel parthenocarpy qtls in tomato.parthenocarpy is the development of the fruit in absence of pollination and/or fertilization. in tomato, parthenocarpy is considered as an attractive trait to solve the problems of fruit setting under unfavorable conditions. we studied the genetics of parthenocarpy in two different lines, il5-1 and ivt-line 1, both carrying solanum habrochaites chromosome segments. parthenocarpy in il5-1 is under the control of two qtls, one on chromosome 4 (pat4.1) and one on chromosome 5 (pat5.1). ivt-line 1 a ...200818231773
novel arthropod repellent, bioud, is an efficacious alternative to deet.for >50 yr, n,n-diethyl-3-methylbenzamide (deet) has been the standard for arthropod repellents and has been an important tool to protect people from disease agents carried by ticks, mosquitoes, and other arthropods. however, some people avoid using deet because of concerns about adverse health effects. in 2007, a new repellent, bioud, with the active ingredient 7.75% 2-undecanone, originally derived from wild tomato (lycopersicon hirsutum dunal f. glabratum c. h. müll) plants, was registered by ...200818826032
two loci from lycopersicon hirsutum la407 confer resistance to strains of clavibacter michiganensis subsp. michiganensis.abstract we used molecular markers to identify quantitative trait loci (qtl) that contribute to resistance to bacterial canker of tomato caused by clavibacter michiganensis subsp. michiganensis. resistance was first identified as a marker-trait association in an inbred backcross (ibc) population derived from crossing lycopersicon hirsutum accession (la407) with l. esculentum. single-marker qtl analysis suggested that at least two loci originating from l. hirsutum la407, rcm 2.0 on chromosome 2 a ...200218943024
the am gene controlling resistance to alfalfa mosaic virus in tomato is located in the cluster of dominant resistance genes on chromosome 6.abstract the dominant gene am from lycopersicon hirsutum f. sp. glabratum pi134417 confers resistance to most strains of alfalfa mosaic virus, including the recently identified necrotic strains. the phenotypic response includes a lack of symptom development following mechanical inoculation of leaves. to study the resistance mechanism controlled by am, biological (back-inoculation to susceptible hosts), serological (double-antibody sandwich, enzyme-linked immunosorbent assay), and molecular (reve ...200418944110
tomato breeding lines resistant and tolerant to tomato yellow leaf curl virus issued from lycopersicon hirsutum.abstract two tomato yellow leaf curl virus (tylcv)-resistant plants from accessions la1777 and la386 of the wild tomato species lycopersicon hirsutum have been crossed. the resulting resistant f1 plants were crossed with the domesticated tomato l. esculentum, and a series of selfing was performed. at each generation, individuals were selected for resistance (no symptoms and undetectable viral dna) and tolerance (no symptoms but with detectable viral dna) following controlled massive and repeated ...199818944868
divergent regulation of terpenoid metabolism in the trichomes of wild and cultivated tomato species.the diversification of chemical production in glandular trichomes is important in the development of resistance against pathogens and pests in two species of tomato. we have used genetic and genomic approaches to uncover some of the biochemical and molecular mechanisms that underlie the divergence in trichome metabolism between the wild species solanum habrochaites la1777 and its cultivated relative, solanum lycopersicum. la1777 produces high amounts of insecticidal sesquiterpene carboxylic acid ...200918997116
complex epistasis for dobzhansky-muller hybrid incompatibility in solanum.we examined the prevalence of interactions between pairs of short chromosomal regions from one species (solanum habrochaites) co-introgressed into a heterospecific genetic background (solanum lycopersicum). of 105 double introgression line (dil) families generated from a complete diallele combination of 15 chromosomal segments, 39 ( approximately 38%) showed evidence for complex epistasis in the form of genotypic and/or allelic marker transmission distortion in dil f(2) populations.200919001290
a novel pathway for sesquiterpene biosynthesis from z,z-farnesyl pyrophosphate in the wild tomato solanum habrochaites.in the wild tomato solanum habrochaites, the sst2 locus on chromosome 8 is responsible for the biosynthesis of several class ii sesquiterpene olefins by glandular trichomes. analysis of a trichome-specific est collection from s. habrochaites revealed two candidate genes for the synthesis of sst2-associated sesquiterpenes. zfps encodes a protein with homology to z-isoprenyl pyrophosphate synthases and sbs (for santalene and bergamotene synthase) encodes a terpene synthase with homology to kaurene ...200919155349
silencing of a single gene in tomato plants resistant to tomato yellow leaf curl virus renders them susceptible to the virus.a reverse-genetics approach was applied to identify genes involved in tomato yellow leaf curl virus (tylcv) resistance, taking advantage of two tomato inbred lines from the same breeding program-one susceptible (s), one resistant (r-that used solanum habrochaites as the source of resistance. cdna libraries from inoculated and non-inoculated r and s plants were compared, postulating that genes preferentially expressed in the r line may be part of the network sustaining resistance to tylcv. furthe ...200919533378
antagonistic epistasis for ecophysiological trait differences between solanum species.epistasis, the nonadditive interaction between loci, is thought to play a role in many fundamental evolutionary processes, including adaptive differentiation and speciation. focusing on species differences in ecophysiological traits, we examined the strength and direction of pairwise epistatic interactions between target chromosomal regions from one species, when co-introgressed into the genetic background of a foreign species. a full diallel cross was performed using 15 near-isogenic lines (nil ...200919659589
identification of solanum habrochaites loci that quantitatively influence tomato fruit ripening-associated ethylene emissions.the phytohormone ethylene is essential for ripening of climacteric fruits such as tomato. while many of the genes responsible for ethylene synthesis and perception have been identified, the regulatory network controlling autocatalytic climacteric ethylene synthesis is not well understood. in order to better understand the regulation of ripening-associated ethylene, we have exploited the genetic variation within solanum sect. lycopersicon. in particular, we have used a near-isogenic population of ...200919680624
multiple biochemical and morphological factors underlie the production of methylketones in tomato trichomes.genetic analysis of interspecific populations derived from crosses between the wild tomato species solanum habrochaites f. sp. glabratum, which synthesizes and accumulates insecticidal methylketones (mk), mostly 2-undecanone and 2-tridecanone, in glandular trichomes, and cultivated tomato (solanum lycopersicum), which does not, demonstrated that several genetic loci contribute to mk metabolism in the wild species. a strong correlation was found between the shape of the glandular trichomes and th ...200919801397
isolation and functional characterization of lycopene beta-cyclase (cyc-b) promoter from solanum habrochaites.carotenoids are a group of c40 isoprenoid molecules that play diverse biological and ecological roles in plants. tomato is an important vegetable in human diet and provides the vitamin a precursor beta-carotene. genes encoding enzymes involved in carotenoid biosynthetic pathway have been cloned. however, regulation of genes involved in carotenoid biosynthetic pathway and accumulation of specific carotenoid in chromoplasts are not well understood. one of the approaches to understand regulation of ...201020380705
an efficient in vitro-inoculation method for tomato yellow leaf curl virus.tomato yellow leaf curl virus (tylcv) is a member of the family geminiviridae, genus begomovirus. to test the infectivity of tylcv in tomato plants, an improved protocol for inoculation of in vitro-cultured tomato plants was developed.201020429892
resistance in tomato and wild relatives to crown and root rot caused by phytophthora capsici.phytophthora capsici causes root, crown, and fruit rot of tomato, a major vegetable crop grown worldwide. the objective of this study was to screen tomato cultivars and wild relatives of tomato for resistance to p. capsici. four p. capsici isolates were individually used to inoculate 6-week-old seedlings (1 g of p. capsici-infested millet seed per 10 g of soilless medium) of 42 tomato cultivars and wild relatives of tomato in a greenhouse. plants were evaluated daily for wilting and death. all p ...201020465418
mineral and trace elements content in 30 accessions of tomato fruits (solanum lycopersicum l.,) and wild relatives (solanum pimpinellifolium l., solanum cheesmaniae l. riley, and solanum habrochaites s. knapp & d.m. spooner).tomato quality and its potential health benefits are directly related to its chemical composition. the characterization of nutritional properties of solanum germplasm is essential to choose suitable donor parents for breeding programs. in this sense, wild species could be very useful for tomato fruit quality genetic improvement. with this objective, in this work, we characterize micronutrients content in eulycopersicon germplasm (20 cultivars of s. lycopersicum l. and 10 accessions of wild relat ...201120526750
enzymatic functions of wild tomato methylketone synthases 1 and 2.the trichomes of the wild tomato species solanum habrochaites subsp. glabratum synthesize and store high levels of methylketones, primarily 2-tridecanone and 2-undecanone, that protect the plants against various herbivorous insects. previously, we identified cdnas encoding two proteins necessary for methylketone biosynthesis, designated methylketone synthase 1 (shmks1) and shmks2. here, we report the isolation of genomic sequences encoding shmks1 and shmks2 as well as the homologous genes from t ...201020605911
tomato-produced 7-epizingiberene and r-curcumene act as repellents to whiteflies.how whiteflies (bemisia tabaci) make the choice for a host plant prior to landing, is not precisely known. here we investigated whether they respond to specific volatiles of tomato. zingiberene and curcumene were purified from solanum habrochaites (pi127826), characterised by nmr and x-ray analysis and identified as 7-epizingiberene and r-curcumene. in contrast, oil from zingiber officinalis contained the stereoisomers zingiberene and s-curcumene, respectively. using a combination of free-choice ...201121074818
comparative functional genomic analysis of solanum glandular trichome types.glandular trichomes play important roles in protecting plants from biotic attack by producing defensive compounds. we investigated the metabolic profiles and transcriptomes to characterize the differences between different glandular trichome types in several domesticated and wild solanum species: solanum lycopersicum (glandular trichome types 1, 6, and 7), solanum habrochaites (types 1, 4, and 6), solanum pennellii (types 4 and 6), solanum arcanum (type 6), and solanum pimpinellifolium (type 6). ...201121098679
polymethylated myricetin in trichomes of the wild tomato species solanum habrochaites and characterization of trichome-specific 3'/5'- and 7/4'-myricetin o-methyltransferases.flavonoids are a class of metabolites found in many plant species. they have been reported to serve several physiological roles, such as in defense against herbivores and pathogens and in protection against harmful ultraviolet radiation. they also serve as precursors of pigment compounds found in flowers, leaves, and seeds. highly methylated, nonglycosylated derivatives of the flavonoid myricetin flavonoid, have been previously reported from a variety of plants, but o-methyltransferases responsi ...201121343428
resistance to tomato yellow leaf curl virus accumulation in the tomato wild relative solanum habrochaites associated with the c4 viral protein.tomato yellow leaf curl disease (tylcd) is a severe threat to tomato crops worldwide and is caused by tomato yellow leaf curl virus (tylcv) and several other begomoviruses (genus begomovirus, family geminiviridae). host plant resistance is the best tylcd control method but limited sources of resistance are available. in this study, two solanum habrochaites tylcd-resistance sources, eelm-388 and eelm-889, were found after a wide germplasm screening and were further characterized. a consistent res ...201121405986
attraction and oviposition of tuta absoluta females in response to tomato leaf volatiles.the tomato leafminer tuta absoluta (lepidoptera: gelechiidae) is a devastating pest of cultivated tomato solanum lycopersicum throughout south and central america and europe. we aimed to characterize the behavioral mechanisms and the chemical cues involved in host selection of t. absoluta females by chemical analysis of tomato leaf volatiles, wind tunnel attraction assays, and oviposition bioassays. tomato leaf odor elicited in mated females upwind orientation flight followed by landing as well ...201121559877
a sensitive bioassay for spider mite (tetranychus urticae) repellency: a double bond makes a difference.choice bioassays were used to determine repellency of homologous n-alkanes (c(8)h(18)-c(21)h(44)) to spider mites. when tested at 400 μg/cm(2), the c(15)-c(19) alkanes were highly repellent; the c(16) n-alkane, n-hexadecane, was most repellent. subsequently the ec(50) values, the concentration at which 50% of the mites were repelled, were determined for the c(15)-c(19) n-alkanes and their analogous 1-n-alkenes (c(15)h(30)-c(19)h(38)). the ec(50) value for 1-heptadecene, the c(17) 1-n-alkene, was ...201121761225
rna-seq discovery, functional characterization, and comparison of sesquiterpene synthases from solanum lycopersicum and solanum habrochaites trichomes.solanum lycopersicum and solanum habrochaites (f. typicum) accession pi127826 emit a variety of sesquiterpenes. to identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing (rna-seq) to obtain the transcriptome of the stem trichomes from these plants. this approach resulted initially in the discovery of six sesquiterpene synthase cdnas from s. lycopersicum and five from s. habrochaites. searches of other databases and the s. l ...201121818683
the fruit cuticles of wild tomato species exhibit architectural and chemical diversity, providing a new model for studying the evolution of cuticle function.the cuticle covers the aerial epidermis of land plants and plays a primary role in water regulation and protection from external stresses. remarkable species diversity in the structure and composition of its components, cutin and wax, have been catalogued, but few functional or genetic correlations have emerged. tomato (solanum lycopersicum) is part of a complex of closely related wild species endemic to the northern andes and the galapagos islands (solanum sect. lycopersicon). although sharing ...201222007785
engineering of bacterial methyl ketone synthesis for biofuels.we have engineered escherichia coli to overproduce saturated and monounsaturated aliphatic methyl ketones in the c(11) to c(15) (diesel) range; this group of methyl ketones includes 2-undecanone and 2-tridecanone, which are of importance to the flavor and fragrance industry and also have favorable cetane numbers (as we report here). we describe specific improvements that resulted in a 700-fold enhancement in methyl ketone titer relative to that of a fatty acid-overproducing e. coli strain, inclu ...201222038610
emergent decarboxylase activity and attenuation of α/β-hydrolase activity during the evolution of methylketone biosynthesis in tomato.specialized methylketone-containing metabolites accumulate in certain plants, in particular wild tomatoes in which they serve as toxic compounds against chewing insects. in solanum habrochaites f. glabratum, methylketone biosynthesis occurs in the plastids of glandular trichomes and begins with intermediates of de novo fatty acid synthesis. these fatty-acyl intermediates are converted via sequential reactions catalyzed by methylketone synthase2 (mks2) and mks1 to produce the n-1 methylketone. we ...201222523203
evolution of tps20-related terpene synthases influences chemical diversity in the glandular trichomes of the wild tomato relative solanum habrochaites.a systematic screen of volatile terpene production in the glandular trichomes of 79 accessions of solanum habrochaites was conducted and revealed the presence of 21 mono- and sesquiterpenes that exhibit a range of qualitative and quantitative variation. hierarchical clustering identified distinct terpene phenotypic modules with shared patterns of terpene accumulation across accessions. several terpene modules could be assigned to previously identified terpene synthase (tps) activities that inclu ...201222563774
characterization of two genes for the biosynthesis of the labdane diterpene z-abienol in tobacco (nicotiana tabacum) glandular trichomes.leaves of tobacco (nicotiana tabacum) are covered with glandular trichomes that produce sucrose esters and diterpenoids in varying quantities, depending on cultivar type. the bicyclic diterpene z-abienol is the major labdanoid present in some oriental tobacco cultivars, where it constitutes a precursor of important flavours and aromas. we describe here the identification and characterization of two genes governing the biosynthesis of z-abienol in n. tabacum. as for other angiosperm labdanoid dit ...201222672125
characterization of a flavonol 3-o-methyltransferase in the trichomes of the wild tomato species solanum habrochaites.the glandular trichomes of the wild tomato species solanum habrochaites accumulate the polymethylated flavonol aglycones, 3,7,3'-o-methyl myricetin, 3,7,3',5'-o-methyl myricetin, and 3,7,3',4',5'-o-methyl myricetin. partially methylated flavonol aglycones and partially methylated flavonol glycones containing a methyl group at the 3 position have been previously reported from a variety of plants. the 3-o-methyltransferase (3-omt) activity has been previously partially purified from plants, but a ...201222711283
synthesis of methyl ketones by metabolically engineered escherichia coli.methyl ketones are a group of highly reduced platform chemicals with widespread applications in the fragrance, flavor and pharmacological industries. current methods for the industrial production of methyl ketones include oxidation of hydrocarbons, but recent advances in the characterization of methyl ketone synthases from wild tomato have sparked interest towards the development of microbial platforms for the industrial production of methyl ketones. a functional methyl ketone biosynthetic pathw ...201222850984
evidence of cryptic introgression in tomato (solanum lycopersicum l.) based on wild tomato species alleles.many highly beneficial traits (e.g. disease or abiotic stress resistance) have been transferred into crops through crosses with their wild relatives. the 13 recognized species of tomato (solanum section lycopersicon) are closely related to each other and wild species genes have been extensively used for improvement of the crop, solanum lycopersicum l. in addition, the lack of geographical barriers has permitted natural hybridization between s. lycopersicum and its closest wild relative solanum p ...201222871151
striking natural diversity in glandular trichome acylsugar composition is shaped by variation at the acyltransferase2 locus in the wild tomato solanum habrochaites.acylsugars are polyesters of short- to medium-length acyl chains on sucrose or glucose backbones that are produced in secretory glandular trichomes of many solanaceous plants, including cultivated tomato (solanum lycopersicum). despite their roles in biotic stress adaptation and their wide taxonomic distribution, there is relatively little information about the diversity of these compounds and the genes responsible for their biosynthesis. in this study, acylsugar diversity was assessed for 80 ac ...201223054567
coevolution and hierarchical interactions of tomato mosaic virus and the resistance gene tm-1.during antagonistic coevolution between viruses and their hosts, viruses have a major advantage by evolving more rapidly. nevertheless, viruses and their hosts coexist and have coevolved, although the processes remain largely unknown. we previously identified tm-1 that confers resistance to tomato mosaic virus (tomv), and revealed that it encodes a protein that binds tomv replication proteins and inhibits rna replication. tm-1 was introgressed from a wild tomato species solanum habrochaites into ...201223093939
differential modulation of photosynthesis, signaling, and transcriptional regulation between tolerant and sensitive tomato genotypes under cold stress.the wild species solanum habrochaites is more cold tolerant than the cultivated tomato (s. lycopersicum). to explore the mechanisms underlying cold tolerance of s. habrochaites, seedlings of s. habrochaites la1777 introgression lines (ils), as well as the two parents, were evaluated under low temperature (4°c). the il la3969 and its donor parent la1777 were found to be more cold tolerant than the recurrent parent s. lycopersicum la4024. the differences in physiology and global gene expression be ...201223226384
evolution of a complex locus for terpene biosynthesis in solanum.functional gene clusters, containing two or more genes encoding different enzymes for the same pathway, are sometimes observed in plant genomes, most often when the genes specify the synthesis of specialized defensive metabolites. here, we show that a cluster of genes in tomato (solanum lycopersicum; solanaceae) contains genes for terpene synthases (tpss) that specify the synthesis of monoterpenes and diterpenes from cis-prenyl diphosphates, substrates that are synthesized by enzymes encoded by ...201323757397
genotypic variability for antioxidant and quality parameters among tomato cultivars, hybrids, cherry tomatoes and wild species.wide germplasm diversity and transferability of antioxidant parameters is the primary requirement for the development of high-antioxidant tomato cultivars. the present study was conducted to screen tomato genotypes including hybrids, varieties, cherry tomatoes, wild species, elite germplasm lines, interspecific hybrids and backcross populations for antioxidant activity and other quality parameters to select high-antioxidant lines with good total soluble solids (tss) for further usage in crop imp ...201424037905
linkage relationships among multiple qtl for horticultural traits and late blight (p. infestans) resistance on chromosome 5 introgressed from wild tomato solanum habrochaites.when the allele of a wild species at a quantitative trait locus (qtl) conferring a desirable trait is introduced into cultivated species, undesirable effects on other traits may occur. these negative phenotypic effects may result from the presence of wild alleles at other closely linked loci that are transferred along with the desired qtl allele (i.e., linkage drag) and/or from pleiotropic effects of the desired allele. previously, a qtl for resistance to phytophthora infestans on chromosome 5 o ...201324122052
pcr-generated molecular markers for the invertase gene and sucrose accumulation in tomato.the green-fruited tomato species, lycopersicon hirsutum, unlike the domesticated red-fruited species, l. esculentum, accumulates sucrose during the final stages of fruit development, concomitant with the loss of soluble acid invertase activity. in order to study the genetic linkage of sucrose accumulation to the invertase gene, part of the invertase gene from l. hirsutum was cloned, sequenced and the sequence compared with the invertase sequence of the red-fruited l. esculentum. several base cha ...199524173076
resistance to powdery mildew (oidium lycopersicum) in lycopersicon hirsutum is controlled by an incompletely-dominant gene ol-1 on chromosome 6.the inheritance of resistance to powdery mildew (oidium lycopersicum) in lycopersicon hirsutum was investigated by disease tests in segregating populations obtained by hybridising tomato (l. esculentum) cv moneymaker with the wild relative l. hirsutum g1.1560. one incompletely dominant gene ol-1 was found to largely control resistance to the disease. to map ol-1, dna pools from seven resistant and ten susceptible f2 plants were analyzed for random amplified polymorphic dna (rapd). with 32 primer ...199424177896
pto3 and pto4: novel genes from lycopersicon hirsutum var. glabratum that confer resistance to pseudomonas syringae pv tomato.accessions of wild lycopersicon germplasm were screened for resistance to pseudomonas syringae pv tomato (p.s. tomato). resistance to both race-0 and race-1 strains of p.s. tomato was identified in l. pimpinellifolium, l. peruvianum and l. hirsutum var. glabratum. resistance to race-0 derived from l. hirsutum var. glabratum (pto3) appeared to be inherited independently of pto1 and pto2. filial and backcross generations derived from interspecific crosses between l. esculentum and l. hirsutum var. ...199424178099
inheritance of zingiberene in lycopersicon.the inheritance of the sesquiterpene zingiberene was analyzed in segregating progeny of interspecific crosses among lycopersicon hirsutum f. hirsutum humb. and bonpl. (hir), l. esculentum mill. cv 'nova' (esc), and l. hirsutum f. glabratum c.h. mull (gla). the presence of zingiberene was inherited as a single dominant gene from hir in f2 and bc progeny of esc x hir, and as a single recessive gene in f2 and bc progeny of gla x hir. the segregation of esc x hir, gla x hir, and esc x gla progeny su ...199324190354
plant regeneration from leaf protoplasts of lycopersicon hirsutum f. hirsutum.a protocol is described for high frequency plant regeneration from isolated leaf protoplasts of several genotypes of the wild tomato species lycopersicon hirsutum f. hirsutum based on modified tomato protoplast culture methods. three to four week old seedlings exposed to a low light pretreatment yielded protoplasts capable of sustained divisions on modified lycopersicon culture medium. plating efficiencies varied from 7.2%-25.9%. colonies were transferred to modified solid greening medium after ...199124213692
chemical imaging of trichome specialized metabolites using contact printing and laser desorption/ionization mass spectrometry.cell transfer by contact printing coupled with carbon-substrate-assisted laser desorption/ionization was used to directly profile and image secondary metabolites in trichomes on leaves of the wild tomato solanum habrochaites. major specialized metabolites, including acyl sugars, alkaloids, flavonoids, and terpenoid acids, were successfully detected in positive ion mode or negative ion mode, and in some cases in both modes. this simple solvent-free and matrix-free sample preparation for mass spec ...201424220760
identification of a short rdna spacer sequence highly specific of a tomato line containing tm-1 gene introgressed from lycopersicon hirsutum.we studied rdna restriction fragment length polymorphism between two tomato lines used for f1 hybrid seed production: line a, containing the tm-1 gene responsible for tobacco mosaic virus tolerance introgressed from the wild species lycopersicon hirsutum, and line b, a tobacco mosaic virus sensitive line. hybridization patterns led to distinct rdna maps with two size classes, 10.4 and 10.7 kb, in line a and a single, 8.9-kb class in line b. size differences were located in the intergenie sequenc ...199024221065
pollen selection for low temperature adaptation in tomato.pollen selection experiments were conducted in tomato to determine the effects of low temperature conditions during pollination on the rate of root elongation of the progeny. pollen was harvested from an f1 interspecific hybrid between a high altitude lycopersicon hirsutum accession and the cultivated tomato l. esculentum. the pollen was applied to stigmas of malesterile l. esculentum plants maintained in growth chambers set at either 12°c/7°c or 24°c/18°c. bc1 seeds from the low and normal temp ...198724240206
2,3-dihydrofarnesoic acid, a unique terpene from trichomes oflycopersicon hirsutum, repels spider mites.lycopersicon hirsutum, a wild relative of the tomato, is highly resistant to arthropod herbivores. both botanic forms ofl. hirsutum, l. hirsutum f.glabratum (c.h. mull.) andl. hirsutum f.typicum (humb. & bonpl.), are resistant to two-spotted spider mites,tetranychus urticae koch. however, leaves and trichome secretions from f.typicum repel mites more so than those from f.glabratum. we have previously demonstrated that trichome secretions from la 1363 and la 1927, accessions of f.typicum, repelle ...199324248790
determination of residues responsible for substrate and product specificity of solanum habrochaites short-chain cis-prenyltransferases.isoprenoids are diverse compounds that have their biosynthetic origin in the initial condensation of isopentenyl diphosphate and dimethylallyl diphosphate to form c10 prenyl diphosphates that can be elongated by the addition of subsequent isopentenyl diphosphate units. these reactions are catalyzed by either cis-prenyltransferases (cpts) or trans-prenyltransferases. the synthesis of volatile terpenes in plants typically proceeds through either geranyl diphosphate (c10) or trans-farnesyl diphosph ...201424254315
influence of life history differences of two tachinid parasitoids ofhelicoverpa zea (boddie) (lepidoptera: noctuidae) on their interactions with glandular trichome/methyl ketone-based insect resistance in tomato.the effects of glandular trichome/methyl ketone (2-tridecanone and 2-undecanone) -based insect resistance in the wild tomato,lycopersicon hirsutum f.glabratum c.h. mull, accession pi 134417, onarchytas marmoratus (townsend) andeucelatoria bryani (sabrosky) (diptera: tachinidae), both parasitoids ofhelicoverpa (=heliothis)zea (boddie) (lepidoptera: noctuidae), were investigated in the laboratory.a. marmoratus deposits larvae (planidia) on the foliage of its host's food plant; planidia attach to p ...199224254953
mortality and inhibition ofhelicoverpa zea egg parasitism rates bytrichogramma in relation to trichome/methyl ketone-mediated insect resistance oflycopersicon hirsutum f. glabratum, accession pi 134417.the glandular trichome/methyl ketone-mediated insect resistance of the wild tomato,lycopersicon hirsutum f. glabratum c.h. mull, accession pi 134417, tomanduca sexta (l.) andleptinotarsa decemlineata (say) was shown to affect adverselytrichogramma pretiosum riley. adultt. pretiosum were killed by direct contact with pi 134417 foliage and by exposure to its volatiles. this effect was greatly reduced or eliminated by removing the exudate of the glandular trichomes from the foliage. 2-tridecanone, ...199124258634
establishment of an in vitro method for evaluating whitefly resistance in tomato.an accurate and simple evaluation method is crucial for identifying whitefly resistance in tomato breeding. we developed an in vitro method for evaluating resistance of tomato leaves and tested this on wild and cultivated tomato varieties. we found that young leaves observed for whitefly oviposition after 8 hours provided appropriate comparative conditions. this method effectively distinguished resistance among tomato cultivars and wild species and also demonstrated significant difference in ovi ...201324273418
low temperature effect on selective fertilization by pollen mixtures of wild and cultivated tomato species.in vitro pollen germination of cultivated tomato, lycopersicon esculentum mill., is inhibited by an ambient temperature of 5°c, more so than pollen from a peruvian ecotype of lycopersicon hirsutum humb. & bonpl. originating from an altitude of 3200 m. the frequency of l. hirsutum gametes contributing to hybrid zygote formation is more than doubled when controlled fertilizations with pollen mixtures of the two species occurs at 12/6°c as compared to crosses with the same mixtures at 24/19°c. the ...198124276484
sesquiterpenoid glycosides from glandular trichomes of the wild tomato relative solanum habrochaites.profiles of terpenoid glycoside metabolites in glandular trichomes of solanum habrochaites la1777 leaves were generated using ultrahigh performance liquid chromatography/time-of-flight mass spectrometry with multiplexing of non-selective collision-induced dissociation (cid). profiling data suggested a diverse group of 52 sesquiterpenoid glycosides, and fragment ions observed in both non-selective cid mass spectra and true tandem mass spectrometry (ms/ms) product ion spectra documented variation ...201424333030
isolation and functional characterization of the shcbf1 gene encoding a crt/dre-binding factor from the wild tomato species solanum habrochaites.plant growth and productivity are greatly affected by low ambient temperature. complex cascades of gene expression in cold stress response are regulated by transcription factors. in this study, a cdna clone, named shcbf1, was isolated from solanum habrochaites seedlings (a wild relative of cultivated tomato). it was classified as one of cbf family members based on multiple sequence alignment. the expression analysis confirmed that shcbf1 was induced by low temperature, high salinity and drought ...201424333684
heterologous expression of methylketone synthase1 and methylketone synthase2 leads to production of methylketones and myristic acid in transgenic plants.some plants produce methylketones as potent defense compounds against various insects. wild tomato (solanum habrochaites), a relative of the cultivated tomato (solanum lycopersicum), synthesizes large amounts of 2-methylketones in its glandular trichomes, but cultivated tomato trichomes contain little or no methylketones. two enzymes, solanum habrochaites methylketone synthase1 (shmks1) and shmks2, are required to convert β-ketoacyl acyl-carrier protein intermediates of the fatty acid biosynthet ...201424390393
[somatic conversion (paramutation) at the sulfurea locus of lycopersicon esculentum mill. : iv. the genotypic determination of the frequency of conversion].1. several lines of lycopersicon esculentum, which are heterozygous for a mutant sulfurea (sulf) allele vary greatly in the percentage of variegated plants among the heterozygotes. this variegation is caused by somatic conversion (paramutation). the different frequency of conversion is due to the presence of different sulf alleles. within the sulfurea (pura) (sulf (pura) ) and sulfurea (variegata) (sulf (vag) ) groups there are different alleles, which - though indistinguishable in homozygous co ...196924435556
comparative structural profiling of trichome specialized metabolites in tomato (solanumlycopersicum) and s. habrochaites: acylsugar profiles revealed by uhplc/ms and nmr.many plants accumulate large quantities of specialized metabolites in secretory glandular trichomes (sgts), which are specialized epidermal cells. in the genus solanum, sgts store a diverse collection of glucose and sucrose esters. profiling of extracts from two accessions (la1777 and la1392) of solanum habrochaites using ultra-high performance liquid chromatography-mass spectrometry (uhplc/ms) revealed wide acylsugar diversity, with up to 11 isomers annotated for each individual elemental formu ...201424772058
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