Publications

effect of genotype and root colonization in biological control of fusarium wilts in pigeonpea and chickpea by pseudomonas aeruginosa pna1.	pseudomonas aeruginosa pna1, an isolate from chickpea rhizosphere in india, protected pigeonpea and chickpea plants from fusarium wilt disease, which is caused by fusarium oxysporum f.sp. ciceris and fusarium udum. inoculation with strain pna1 significantly reduced the incidence of fusarium wilt in pigeonpea and chickpea on both susceptible and moderately tolerant genotypes. however, strain pna1 protected the plants from fusarium wilt until maturity only in moderately tolerant genotypes of pigeo ...	2003	12718396
molecular mapping of fusarium oxysporum f. sp. ciceris race 3 resistance gene in chickpea.	sequence-tagged microsatellite site (stms) and sequence-tagged site (sts) markers linked closely to fusarium oxysporum f. sp. ciceris race 3 resistance gene in chickpea were identified, and linkage between three wilt resistance genes was elucidated. the resistance to race 3 in chickpea germplasm accession wr-315 was inherited as a single gene, designated foc-3, in 100 f(7) recombinant inbred lines derived from the cross of wr-315 (resistant) x c-104 (susceptible). the foc-3 gene was mapped 0.6 c ...	2004	14689189
the fusarium oxysporum f. sp. ciceris/cicer arietinum pathosystem: a case study of the evolution of plant-pathogenic fungi into races and pathotypes.	the use of resistant cultivars is one of the most practical and cost-efficient strategies for managing plant diseases. however, the efficiency of resistant cultivars in disease management is limited by pathogenic variability in pathogen populations. knowledge of the evolutionary history and potential of the pathogen population may help to optimize the management of disease-resistance genes, irrespective of the breeding strategy used for their development. in this review, we examine the diversity ...	2004	15248157
extracellular xylanases from two pathogenic races of fusarium oxysporum f. sp. ciceris: enzyme production in culture and purification and characterization of a major isoform as an alkaline endo-beta-(1,4)-xylanase of low molecular weight.	fusarium oxysporum f. sp. ciceris, the causal agent of fusarium wilt of chickpea, comprises eight pathogenic races and two pathotypes. races 0 and 5, representative of the least virulent yellowing pathotype and the most virulent wilt pathotype, respectively, produced extracellular xylanases when grown on minimal medium supplemented with either 1% commercial birchwood xylan or 0.3% chickpea cell walls. the pattern of extracellular proteins analysed by denaturing polyacrylamide gel electrophoresis ...	2005	15928976
treatment of chickpea with rhizobium isolates enhances the expression of phenylpropanoid defense-related genes in response to infection by fusarium oxysporum f. sp. ciceris.	differential expression of phenylalanine ammonia-lyase (pal), chalcone synthase (chs) and isoflavone reductase (ifr) genes involved in phenylpropanoids metabolism was investigated using northern blot analyses in chickpea seedlings bacterized with rhizobium isolates (pchdms and pch43) and further challenged with fusarium oxysporum f. sp. ciceris (foc) race 0. gene activation patterns in the moderately resistant accession inrat87/1 were compared with those exhibited by the susceptible accession il ...	2007	17544286
virulence analysis and oligonucleotide fingerprinting to detect diversity among indian isolates of fusarium oxysporum f. sp. ciceris causing chickpea wilt.	virulence analysis of 64 isolates of fusarium oxysporum f. sp. ciceris causing chickpea wilt collected from major chickpea growing states of india on 14 varieties, including 10 international differentials revealed that the isolates from each state were highly variable. based on the reactions on international differentials, more than one race was found to be prevalent in every state. majority of the isolates were not matched with the race specific reactions. therefore, some of the cultivars, name ...	2008	18266072
integrated management of fusarium wilt of chickpea with sowing date, host resistance, and biological control.	abstract a 3-year experiment was conducted in field microplots infested with fusarium oxysporum f. sp. ciceris race 5 at córdoba, spain, in order to assess efficacy of an integrated management strategy for fusarium wilt of chickpea that combined the choice of sowing date, use of partially resistant chickpea genotypes, and seed and soil treatments with biocontrol agents bacillus megaterium rgaf 51, b. subtilis gb03, nonpathogenic f. oxysporum fo 90105, and pseudomonas fluorescens rg 26. advancing ...	2004	18943071
development of a specific polymerase chain reaction-based assay for the identification of fusarium oxysporum f. sp. ciceris and its pathogenic races 0, 1a, 5, and 6.	abstract specific primers and polymerase chain reaction (pcr) assays that identify fusarium oxysporum f. sp. ciceris and each of the f. oxysporum f. sp. ciceris pathogenic races 0, 1a, 5, and 6 were developed. f. oxysporum f. sp. ciceris- and race-specific random amplified polymorphic dna (rapd) markers identified in a previous study were cloned and sequenced, and sequence characterized amplified region (scar) primers for specific pcr were developed. each cloned rapd marker was characterized by ...	2003	18943135
quantitative modeling of the effects of temperature and inoculum density of fusarium oxysporum f. sp. ciceris races 0 and 5 on development of fusarium wilt in chickpea cultivars.	abstract races 0 (foc-0) and 5 (foc-5) of fusarium oxysporum f. sp. ciceris differ in virulence and induce yellowing or wilting syndrome, respectively, in chickpea. we modeled the combined effects of soil temperature and inoculum density of foc-0 and foc-5 on disease developed in chickpea cvs. p-2245 and pv-61 differing in susceptibility to those races, using quantitative nonlinear models. disease development over time in the temperature range of 10 to 30 degrees c and inoculum densities between ...	2007	18943575
interactions between meloidogyne artiellia, the cereal and legume root-knot nematode, and fusarium oxysporum f. sp. ciceris race 5 in chickpea.	abstract in the mediterranean basin, fusarium oxysporum f. sp. ciceris and the root-knot nematode meloidogyne artiellia coinfect chickpea. the influence of root infection (after inoculation with 20 nematode eggs and second-stage juveniles per gram of soil) by two m. artiellia populations, from italy and syria, on the reaction of chickpea lines and cultivars with partial resistance to fusarium wilt (ca 252.10.1.om, ca 255.2.5.0, cps 1, and pv 61) and with complete resistance to f. oxysporum f. sp ...	2003	18943615
stepwise evolution of races in fusarium oxysporum f. sp. ciceris inferred from fingerprinting with repetitive dna sequences.	abstract plant pathogens often exhibit variation in virulence, the ability to cause disease on host plants with specific resistance, evident from the diversity of races observed within pathogen species. the evolution of races in asexual fungal pathogens has been hypothesized to occur in a stepwise fashion, in which mutations to virulence accumulate sequentially in clonal lineages, resulting in races capable of overcoming multiple host plant resistance genes or multiple resistant cultivars. in th ...	2004	18943970
influence of temperature and inoculum density of fusarium oxysporum f. sp. ciceris on suppression of fusarium wilt of chickpea by rhizosphere bacteria.	the effects of temperature and inoculum density of fusarium oxysporum f. sp. ciceris race 5 on suppression of fusarium wilt in chickpea (cicer arietinum) cv. pv 61 by seed and soil treatments with rhizobacteria isolated from the chickpea rhizosphere were studied in a model system. disease development over a range of temperatures (20, 25, and 30 degrees c) and inoculum densities (25 to 1,000 chlamydospores per gram of soil) was described by the gompertz model. the gompertz relative rate of diseas ...	2001	18944039
infection by meloidogyne artiellia does not break down resistance to races 0, 1a, and 2 of fusarium oxysporum f. sp. ciceris in chickpea genotypes.	fusarium oxysporum f. sp. ciceris, and the root-knot nematode meloidogyne artiellia, coinfect chickpea crops in several countries of the mediterranean basin. the influence of root infection by m. artiellia on the reactions of chickpea genotypes with different reaction to infection with f. oxysporum f. sp. ciceris races 0, 1a, and 2 was investigated under controlled environmental conditions. results demonstrated that co-infection of chickpea genotypes resistant to specific fungal races by m. arti ...	2008	18944296
yield loss in chickpeas in relation to development of fusarium wilt epidemics.	abstract development of 108 epidemics of fusarium wilt of chickpea caused by fusarium oxysporum f. sp. ciceris were studied on cvs. p-2245 and pv-61 in field microplots artificially infested with races 0 and 5 of f. oxysporum f. sp. ciceris in 1986 to 1989. disease progression data were fitted to the richards model using nonlinear regression. the shape parameter was influenced primarily by date of sowing and, to a lesser extent, by chick-pea cultivars and races of f. oxysporum f. sp. ciceris. fu ...	2000	18944431
effect of sowing date, host cultivar, and race of fusarium oxysporum f. sp. ciceris on development of fusarium wilt of chickpea.	abstract microplots experiments were carried out at córdoba, southern spain, from 1986 to 1989 to determine the effects of sowing date in the management of fusarium wilt of chickpea as influenced by virulence of the pathogen race and by cultivar susceptibility. a total of 108 epidemics of the disease were described, analyzed, and compared to assess the degree of disease control. the epidemics were characterized by five curve elements: final disease intensity index (dii), standardized area under ...	1998	18944837
interactions of pratylenchus thornei and fusarium oxysporum f. sp. ciceris on chickpea.	abstract fusarium oxysporum f. sp. ciceris and the root-lesion nematode pratylenchus thornei coinfect chickpeas in southern spain. the influence of root infection by p. thornei on the reaction of fusarium wilt-susceptible (cps 1 and pv 61) and wilt-resistant (uc 27) chickpea cultivars to f. oxysporum f. sp. ciceris race 5 was investigated under controlled and field conditions. severity of fusarium wilt was not modified by coinfection of chickpeas by p. thornei and f. oxysporum f. sp. ciceris, in ...	1998	18944890
integration of new caps and dcaps-rga markers into a composite chickpea genetic map and their association with disease resistance.	a composite linkage map was constructed based on two interspecific recombinant inbred line populations derived from crosses between cicer arietinum (ilc72 and iccl81001) and cicer reticulatum (cr5-10 or cr5-9). these mapping populations segregate for resistance to ascochyta blight (caused by ascochyta rabiei), fusarium wilt (caused by fusarium oxysporum f. sp. ciceris) and rust (caused by uromyces ciceris-arietini). the presence of single nucleotide polymorphisms in ten resistance gene analogs ( ...	2009	19034411
changes in the redox status of chickpea roots in response to infection by fusarium oxysporum f. sp. ciceris: apoplastic antioxidant enzyme activities and expression of oxidative stress-related genes.	activity levels of oxidative stress-related enzymes in the root apoplast during the interaction of wr315 (resistant) and jg62 (susceptible) chickpeas (cicer arietinum l.) with the highly virulent race 5 of fusarium oxysporum f. sp. ciceris were compared. because this fungus develops asymptomatic infections in the chickpea root cortex in both susceptible and resistant plants, but only intrudes into the root xylem in the susceptible variety, the interactions were compared at three specific stages ...	2009	19228326
identification of indian pathogenic races of fusarium oxysporum f. sp. ciceris with gene specific, its and random markers.	in this study we demonstrate the synergistic use of gene-specific markers, its-rflp, issr and aflp for distinguishing indian f. oxysporum f. sp. ciceris races. we also report for the first time that f. oxysporum f. sp. ciceris race 3, a wilt pathogen of chickpea in india, is actually f. proliferatum based on phylogenetic analysis with ef-1alpha sequence data. f. oxysporum f. sp. ciceris races 1, 2 and 4 were easily distinguished from "race 3" (f. proliferatum) by pcr amplification with oligonucl ...	2009	19623928
a molecular insight into the early events of chickpea (cicer arietinum) and fusarium oxysporum f. sp. ciceri (race 1) interaction through cdna-aflp analysis.	wilt of chickpea caused by fusarium oxysporum f. sp. ciceris is one of the most severe diseases of chickpea throughout the world. variability of pathotypes of f. oxysporum f. sp. ciceris and breakdown of natural resistance are the main hindrances to developing resistant plants by applying resistant breeding strategies. additionally, lack of information of potential resistant genes limits gene-transfer technology. a thorough understanding of fusarium spp.-chickpea interaction at a cellular and mo ...	2009	19821728
development of chickpea near-isogenic lines for fusarium wilt.	four pairs of near-isogenic lines (nils) of chickpea with resistance/susceptibility to fusarium oxysporum f. sp. ciceris (foc) have been developed in this study. these lines were produced by searching in advanced recombinant inbred lines (rils) that are segregating for foc race 5 based on a phenotypic screening. the sequence tagged microsatellite (stms) marker ta59, closely linked to wilt resistance genes on linkage group 2 (lg2) of the chickpea map, was used to assist the selection of resistant ...	2010	20652529
in planta and soil quantification of fusarium oxysporum f. sp. ciceris and evaluation of fusarium wilt resistance in chickpea with a newly developed quantitative polymerase chain reaction assay.	fusarium wilt of chickpea caused by fusarium oxysporum f. sp. ciceris can be managed by risk assessment and use of resistant cultivars. a reliable method for the detection and quantification of f. oxysporum f. sp. ciceris in soil and chickpea tissues would contribute much to implementation of those disease management strategies. in this study, we developed a real-time quantitative polymerase chain reaction (q-pcr) protocol that allows quantifying f. oxysporum f. sp. ciceris dna down to 1 pg in s ...	2011	21219129
its-rflp fingerprinting and molecular marker for detection of fusarium oxysporum f.sp. ciceris.	genetic diversity of 11 representative isolates of fusarium oxysporum f.sp. ciceris causing chickpea wilt was determined through internal transcribed spacer (its) region of the ribosomal dna-restriction fragment length polymorphism (its-rflp). its1+5.8s+its2 regions of the isolates were amplified with a set of primers its1 and its4 and amplified products were digested with 4 restriction enzymes (alui, mboi, rsai, msei). six different kinds of its-rflp patterns were obtained. the its region of th ...	2010	21253911
a proteomic study of in-root interactions between chickpea pathogens: the root-knot nematode meloidogyne artiellia and the soil-borne fungus fusarium oxysporum f. sp. ciceris race 5.	fusarium wilt caused by the fungus fusarium oxysporum f. sp. ciceris (foc) is the main soil-borne disease limiting chickpea production. management of this disease is achieved mainly by the use of resistant cultivars. however, co-infection of a foc-resistant plant by the fungus and the root-knot nematode meloidogyne artiellia (ma) causes breakdown of the resistance and thus limits its efficacy in the control of fusarium wilt. in this work we aimed to reveal key aspects of chickpea:foc:ma interact ...	2011	21640211