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effect of temperature on geminivirus-induced rna silencing in plants.short-interfering rnas (sirnas), the molecular markers of posttranscriptional gene silencing (ptgs), are powerful tools that interfere with gene expression and counter virus infection both in plants and animals. here, we report the effect of temperature on geminivirus-induced gene silencing by quantifying virus-derived sirnas and by evaluating their distribution along the virus genome for isolates of five species of cassava geminiviruses in cassava (manihot esculenta, crantz) and nicotiana benth ...200516040661
characterization of the cassava geminivirus transcription activation protein putative nuclear localization signal.the geminivirus transcription activation protein (trap) localizes to the nucleus and contains a putative nuclear localization signal (nls) ((28)prrrr(32)) on the n-terminus. the role of individual residues of this putative nls on nuclear localization and symptom induction was investigated using trap of east african cassava mosaic cameroon virus (eacmcv). subcellular localization was conducted using the green fluorescent protein (gfp). results showed that the proline residue at position 28 (pro-2 ...200919665038
resistance gene analogs involved in tolerant cassava--geminivirus interaction that shows a recovery phenotype.the current literature describes recovery from virus-induced symptoms as a rna silencing defense, but immunity-related genes, including the structurally specific resistance gene analogs (rgas) that may play a key role in tolerance and recovery is not yet reported. in this study, the transcriptome data of tolerant cassava tme3 (which exhibits a recovery phenotype) and susceptible cassava t200 infected with south african cassava mosaic virus were explored for rgas. putative resistance protein anal ...201526370397
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