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morphology of the cyst of giardia microti by light and electron microscopy.cysts of giardia microti, isolated from feces and intestinal contents of microtus ochrogaster, were examined by light and electron microscopy. these cysts differed morphologically from cysts of other g. duodenalis morphological types in that these cysts often contained two apparently differentiated trophozoites with mature ventral discs. cysts more closely resembling those reported for g. lamblia and g. muris were in greater abundance in preparations made from intestinal contents and were interp ...19883285001
a comparison of giardia microti and spironucleus muris cysts in the vole: an immunocytochemical, light, and electron microscopic study.we have shown that cysts of the genus spironucleus share many common morphological features with giardia cysts including: 2-4 nuclei, flagellar axonemes, a distinct cyst wall, and they even display the same immunostaining as giardia cysts when labeled with antibodies specific for giardia cyst wall. a direct comparison of spironucleus muris and giardia microti cysts have revealed that cysts of s. muris are significantly smaller than cysts of g. miroti. at the ultrastructural level, the cyst walls ...19883288741
the ultrastructure of the intestinal microvillous border in the common vole (microtus arvalis) naturally infected with giardia microti.the anterior jejunum from common vole naturally infected with giardia microti (kofoid et christiansen, 1915) was examined by tem and compared with the anterior jejunum from control (metronidazole-treated, giardia-free) common voles (pallas, 1778). giardia microti infection resulted in significant diffuse shortening of microvilli and significant greater microvillous diameters. in addition, deformations of the microvilli were observed at the margin of the ventral disc. the microvilli attached to t ...19947883257
the sequence of giardia small subunit rrna shows that voles and muskrats are parasitized by a unique species giardia microti.the small subunit ribosomal rna (eukaryotic 16s rrna) gene from giardia trophozoites, isolated from 8 different prairie voles and 8 different muskrats, was amplified by the polymerase chain reaction. the 16s rdna was sequenced in its entirety for 2 prairie vole and 2 muskrat giardia. in addition, the 5' 500 nucleotides of the 16s rdna from giardia isolates from each of 6 voles and 6 muskrats were amplified and sequenced. the results show that giardia from voles and muskrats are very similar to e ...19989576501
genotyping of giardia lamblia isolates from humans in china and korea using ribosomal dna sequences.genetic characterization of a total of 15 giardia lamblia isolates, 8 from anhui province, china (all from purified cysts) and 7 from seoul, korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal dna (eukaryotic 16s rdna). phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other ...200010958483
identification of zoonotic giardia genotypes in fish.apart from a single record in a shark, there have been no published studies conducted on giardia genotypes in fish. the present study investigated the prevalence of giardia in cultured fingerlings (n=227), wild freshwater (n=227) and wild marine/estuarine species (n=255) of fish in western australia by pcr amplification at the 18s rrna, glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and beta-giardin (bg) loci. results revealed a low prevalence of giardia, 3.8% (27/709), in fish ...201020026124
prevalence and molecular typing of giardia spp. in captive mammals at the zoo of zagreb, croatia.a total of 131 faecal samples from 57 mammalian species housed at the zoo of zagreb, croatia, were tested for the presence of giardia spp. cysts using epifluorescence microscopy. the overall prevalence (29%) was high, yet all animals were asymptomatic at the time of sampling. positive samples were characterized by pcr and sequence analysis of both conserved and variable loci, for the identification of giardia species and g. duodenalis assemblages and genotypes. assemblages a and c were identifie ...201020970259
a large survey of croatian wild mammals for giardia duodenalis reveals a low prevalence and limited zoonotic potential.abstract wild mammals are considered an important source of potentially zoonotic giardia duodenalis parasites, yet surprisingly little information is available on the actual prevalence and the genetic identity of the species they harbor. a large survey was conducted in croatia by collecting 832 fecal samples from red deer (cervus elaphus, n = 374), roe deer (capreolus capreolus, n = 21), wild boars (sus scrofa, n = 144), foxes (vulpes vulpes, n = 66), bears (ursus arctos, n = 19), wolves (canis ...201021142957
molecular characterization of giardia psittaci by multilocus sequence analysis.multilocus sequence analyses targeting small subunit ribosomal dna (ssu rdna), elongation factor 1 alpha (ef1α), glutamate dehydrogenase (gdh), and beta giardin (β-giardin) were performed on giardia psittaci isolates from three budgerigars (melopsittacus undulates) and four barred parakeets (bolborhynchus lineola) kept in individual households or imported from overseas. nucleotide differences and phylogenetic analyses at four loci indicate the distinction of g. psittaci from the other known giar ...201222921500
confirmation of a unique species of giardia, parasitic in the quenda (isoodon obesulus).the 'quenda genotype' of giardia was first identified in quenda (syn. southern brown bandicoots, isoodon obesulus) in western australia in 2004. we aimed to formally describe this genotype as a species of giardia, giardia peramelis. seventy five faecal samples positive for g. peramelis were obtained from quenda within the statistical division of perth, western australia. these samples were used in morphological and molecular characterisation of g. peramelis. pcr amplification and sequencing was ...201628560164
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